AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0750210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750210 MtrunA17_Chr5g0396281 98.800 250 3 0 1 250 1 250 0.0 518
Msa0750210 MtrunA17_Chr2g0282301 35.354 198 124 2 29 222 71 268 1.88e-42 146
Msa0750210 MtrunA17_Chr4g0047231 34.483 232 140 6 12 240 68 290 7.27e-42 145
Msa0750210 MtrunA17_Chr4g0040171 40.331 181 105 3 44 222 66 245 9.83e-41 141
Msa0750210 MtrunA17_Chr4g0047221 32.759 232 143 5 12 240 122 343 3.68e-40 142
Msa0750210 MtrunA17_Chr2g0282311 30.973 226 149 3 18 240 65 286 2.62e-39 138
Msa0750210 MtrunA17_Chr4g0075591 32.673 202 111 6 45 243 79 258 2.87e-30 114
Msa0750210 MtrunA17_Chr4g0075611 32.512 203 113 6 45 243 94 276 2.13e-29 112
Msa0750210 MtrunA17_Chr7g0251511 31.293 147 97 3 94 240 128 270 7.76e-28 108
Msa0750210 MtrunA17_Chr1g0166241 34.677 124 75 1 97 220 153 270 1.78e-24 100
Msa0750210 MtrunA17_Chr2g0289701 32.768 177 100 5 65 240 114 272 2.13e-24 99.4
Msa0750210 MtrunA17_Chr8g0384101 33.594 128 84 1 94 220 79 206 8.88e-24 96.7
Msa0750210 MtrunA17_Chr1g0188091 33.333 141 83 2 97 232 175 309 7.50e-23 95.9
Msa0750210 MtrunA17_Chr1g0184881 28.646 192 120 4 29 220 111 285 2.35e-22 94.0
Msa0750210 MtrunA17_Chr8g0339051 31.544 149 95 3 99 241 133 280 1.33e-21 91.3
Msa0750210 MtrunA17_Chr7g0234691 34.109 129 83 2 95 222 169 296 3.82e-21 90.5
Msa0750210 MtrunA17_Chr7g0260551 32.812 128 78 3 98 225 161 280 1.57e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750210 AT4G32480.1 36.548 197 123 2 28 222 45 241 2.67e-46 156
Msa0750210 AT2G20670.1 35.096 208 130 3 44 249 63 267 4.69e-43 148
Msa0750210 AT3G07350.1 35.714 126 81 0 97 222 135 260 6.21e-30 114
Msa0750210 AT3G25240.1 38.760 129 78 1 95 222 113 241 1.26e-28 110
Msa0750210 AT2G39650.2 33.071 127 85 0 94 220 45 171 1.61e-26 103
Msa0750210 AT2G39650.1 33.071 127 85 0 94 220 107 233 2.21e-26 104
Msa0750210 AT4G14620.1 34.677 124 75 1 97 220 143 260 9.50e-26 103
Msa0750210 AT2G38820.2 36.508 126 73 2 95 220 158 276 1.30e-24 100
Msa0750210 AT1G12030.1 33.140 172 92 5 52 222 81 230 4.69e-24 98.2
Msa0750210 AT3G22970.2 33.871 124 76 1 97 220 57 174 3.97e-23 95.1
Msa0750210 AT3G22970.1 33.871 124 76 1 97 220 168 285 1.82e-22 95.1
Msa0750210 AT1G62420.1 32.540 126 82 1 98 223 109 231 7.74e-22 92.0
Msa0750210 AT3G54550.1 27.465 142 93 2 101 238 148 283 1.02e-16 78.2
Msa0750210 AT1G77145.1 29.545 132 81 2 101 220 79 210 1.02e-14 72.0
Msa0750210 AT1G77160.2 29.412 136 83 3 97 220 26 160 1.26e-14 70.9
Msa0750210 AT1G77160.1 29.545 132 81 2 101 220 84 215 1.73e-14 71.6
Msa0750210 AT1G77145.2 35.789 95 57 1 130 220 59 153 7.66e-14 68.6

Find 83 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTGTTAGGAAATTT+TGG 0.191126 5_2:+3612850 Msa0750210:intron
TGATGAACGTGTTTATATAT+AGG 0.242613 5_2:-3611456 None:intergenic
TTTGACATTCATTTCCCTTT+AGG 0.255256 5_2:+3613826 Msa0750210:three_prime_UTR
GATGAACGTGTTTATATATA+GGG 0.258259 5_2:-3611455 None:intergenic
GCAAACAAGACACAAGTTTC+TGG 0.293899 5_2:-3613464 None:intergenic
TTTCCTTCAACCACTTTGTT+TGG 0.299739 5_2:+3611519 Msa0750210:five_prime_UTR
AGTACCATTTCTGATTGAAT+TGG 0.335912 5_2:+3613398 Msa0750210:CDS
TCAAAATCTACCACTGGAAT+TGG 0.346284 5_2:-3613732 None:intergenic
AACACAAAGGACTTTGTATT+GGG 0.356331 5_2:+3611746 Msa0750210:CDS
AATACAAAGTCCTTTGTGTT+AGG 0.357106 5_2:-3611743 None:intergenic
GAATTTGCTTGTGTTGTTGA+AGG 0.360556 5_2:+3614032 Msa0750210:three_prime_UTR
GAGTCATGTGTCGCGTTTCT+AGG 0.366332 5_2:+3613967 Msa0750210:three_prime_UTR
CCAACCTTTGCACATCTAGA+TGG 0.378351 5_2:+3613038 Msa0750210:CDS
GGCTTCCCAACATAAAATTC+AGG 0.380679 5_2:-3613486 None:intergenic
TAACACAAAGGACTTTGTAT+TGG 0.383816 5_2:+3611745 Msa0750210:CDS
TTGGAGCGACACCATATGAT+TGG 0.387836 5_2:+3612869 Msa0750210:CDS
GAAGGAGAAGAAAATGTATC+TGG 0.388065 5_2:+3613563 Msa0750210:CDS
AATTCCAATTCAATCAGAAA+TGG 0.405930 5_2:-3613402 None:intergenic
TAGAAGCATGAAATTGAAAA+GGG 0.407876 5_2:-3614084 None:intergenic
GTAGAAGCATGAAATTGAAA+AGG 0.412944 5_2:-3614085 None:intergenic
AAAACCACAAATGAATTTCC+AGG 0.430404 5_2:+3613061 Msa0750210:CDS
TTCATGCAAATGAAGTGGTC+AGG 0.435123 5_2:+3613606 Msa0750210:CDS
GTGGTGCAATAATGAAAGTT+AGG 0.436885 5_2:-3612965 None:intergenic
TTGACTGCTACCACTGCATT+AGG 0.442412 5_2:-3613690 None:intergenic
GGAATATATTATAAAGCATA+TGG 0.454576 5_2:+3613794 Msa0750210:three_prime_UTR
TAGATTCACTTGCTTGTGTA+TGG 0.461506 5_2:-3613647 None:intergenic
TGGAGCGACACCATATGATT+GGG 0.464808 5_2:+3612870 Msa0750210:CDS
AGAAATACAAAGAGGGAAGA+AGG 0.466260 5_2:-3611559 None:intergenic
AGCCACTAGTCTTCTCAAAC+AGG 0.471489 5_2:-3612987 None:intergenic
CATAGACAATATCAAAGCTT+CGG 0.471622 5_2:+3612922 Msa0750210:CDS
TAACATAACATAGGACCTAA+AGG 0.473739 5_2:-3613841 None:intergenic
ACCTCCTGGAAATTCATTTG+TGG 0.474315 5_2:-3613065 None:intergenic
AGAAAATGTATCTGGCTCCT+TGG 0.483912 5_2:+3613571 Msa0750210:CDS
TCAATTTCGACCCAATCATA+TGG 0.486618 5_2:-3612880 None:intergenic
AACATAACATAGGACCTAAA+GGG 0.488803 5_2:-3613840 None:intergenic
AAATGTATCTGGCTCCTTGG+AGG 0.492634 5_2:+3613574 Msa0750210:CDS
ATGCCAAACAAAGTGGTTGA+AGG 0.501480 5_2:-3611522 None:intergenic
GCTTGTGTATGGTGATATGT+TGG 0.515521 5_2:-3613636 None:intergenic
TCCCCTTCAAAATCTACCAC+TGG 0.525171 5_2:-3613738 None:intergenic
TGCTTTGTTCAGACAAGCAT+AGG 0.542553 5_2:-3611704 None:intergenic
TATTGTGTTGTTGAAGAGCA+TGG 0.543496 5_2:+3611614 Msa0750210:exon
AAACAAAGTGGTTGAAGGAA+AGG 0.544360 5_2:-3611517 None:intergenic
ACAATCGCGGTTAAGTACTC+TGG 0.547258 5_2:-3613507 None:intergenic
ACACAATAGAAACTACTACT+AGG 0.551480 5_2:-3613884 None:intergenic
GACAAGCATAGGTCCTCAAG+AGG 0.554518 5_2:-3611693 None:intergenic
CCATCTAGATGTGCAAAGGT+TGG 0.559415 5_2:-3613038 None:intergenic
TGCTGCAAAGAAATCAATGA+AGG 0.567461 5_2:+3613545 Msa0750210:CDS
TTCGTTTAGAAATACAAAGA+GGG 0.570750 5_2:-3611566 None:intergenic
CAGTTCCAGATTGCAAAAGC+AGG 0.571376 5_2:+3613432 Msa0750210:CDS
AGAGCTGCATGCCCCTCTTG+AGG 0.571583 5_2:+3611680 Msa0750210:CDS
GAAATACAAAGAGGGAAGAA+GGG 0.572552 5_2:-3611558 None:intergenic
GCATGAAATTGAAAAGGGGA+AGG 0.575445 5_2:-3614079 None:intergenic
AGGGGAAGGAAGAATGCAAA+GGG 0.579820 5_2:-3614065 None:intergenic
ATTCGTTTAGAAATACAAAG+AGG 0.580767 5_2:-3611567 None:intergenic
AAAGCAAGTAAGTTTCACAA+TGG 0.583102 5_2:+3613918 Msa0750210:three_prime_UTR
GCAGTTTCATGCAAATGAAG+TGG 0.583111 5_2:+3613601 Msa0750210:CDS
ATTACATCATAACATAACAT+AGG 0.585846 5_2:-3613850 None:intergenic
GCTGCTATGCTATGTGAAAG+AGG 0.587932 5_2:+3613007 Msa0750210:CDS
CAAAGAGGGAAGAAGGGCTA+AGG 0.591638 5_2:-3611552 None:intergenic
ATTCCTACGTACTAATGAAG+AGG 0.597235 5_2:-3613776 None:intergenic
TTCAGGAGCTCCTAATGCAG+TGG 0.598514 5_2:+3613680 Msa0750210:CDS
GCAAGTAAGTTTCACAATGG+AGG 0.598818 5_2:+3613921 Msa0750210:three_prime_UTR
TTCTTCAATTCCAATTCCAG+TGG 0.613919 5_2:+3613722 Msa0750210:three_prime_UTR
TTCTAAACGAATAACAAAGA+GGG 0.616469 5_2:+3611578 Msa0750210:five_prime_UTR
TTTCTAAACGAATAACAAAG+AGG 0.618095 5_2:+3611577 Msa0750210:five_prime_UTR
ACAAGCATAGGTCCTCAAGA+GGG 0.619122 5_2:-3611692 None:intergenic
TCGAAATTGAGACAAGAGGT+TGG 0.623564 5_2:+3612893 Msa0750210:CDS
TGGGTCGAAATTGAGACAAG+AGG 0.625191 5_2:+3612889 Msa0750210:CDS
AGAGAAAATGCCAAACAAAG+TGG 0.639651 5_2:-3611529 None:intergenic
TGAACAAAGCAATGACTCTG+AGG 0.640823 5_2:+3611716 Msa0750210:CDS
CACCTGTTTGAGAAGACTAG+TGG 0.640946 5_2:+3612985 Msa0750210:CDS
AGAAGCATGAAATTGAAAAG+GGG 0.641184 5_2:-3614083 None:intergenic
AAGGGGAAGGAAGAATGCAA+AGG 0.644340 5_2:-3614066 None:intergenic
CAAGCATAGGTCCTCAAGAG+GGG 0.646120 5_2:-3611691 None:intergenic
CACTAGTCTTCTCAAACAGG+TGG 0.650169 5_2:-3612984 None:intergenic
ATCTCAGCTAGCACTTCTTG+AGG 0.650841 5_2:+3611770 Msa0750210:CDS
ACACACAAGTCGAACAATCG+CGG 0.653266 5_2:-3613520 None:intergenic
ATGTTGTCAACAGAGAGTAG+AGG 0.653934 5_2:+3614003 Msa0750210:three_prime_UTR
CAATAGAAACTACTACTAGG+TGG 0.667165 5_2:-3613881 None:intergenic
AGACCTCTTCATTAGTACGT+AGG 0.670566 5_2:+3613773 Msa0750210:three_prime_UTR
TCTCTGTTGACAACATCTAG+TGG 0.677328 5_2:-3613996 None:intergenic
CTGAGGAACACCTAACACAA+AGG 0.685583 5_2:+3611733 Msa0750210:CDS
ACCACAAATGAATTTCCAGG+AGG 0.741742 5_2:+3613064 Msa0750210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATTTTATTTTATTT+TGG - chr5_2:3614110-3614129 None:intergenic 0.0%
!!! TAAAATCATTTTATGTAATT+AGG + chr5_2:3612538-3612557 Msa0750210:intron 10.0%
!! TTTATAATAACTAACTATTT+AGG - chr5_2:3613123-3613142 None:intergenic 10.0%
!!! ACAAAATAATGAATTTTTAT+TGG + chr5_2:3613237-3613256 Msa0750210:intron 10.0%
!!! AGTAATTAATTTTAGATTAA+AGG - chr5_2:3613272-3613291 None:intergenic 10.0%
!! TATTACATTAAGTTCTTTAA+AGG + chr5_2:3612071-3612090 Msa0750210:intron 15.0%
!!! TATTAATTTTTTGACTAAGT+TGG + chr5_2:3612128-3612147 Msa0750210:intron 15.0%
!!! TTTAAAGAATTCATTTTCAT+TGG - chr5_2:3612348-3612367 None:intergenic 15.0%
!!! TTCTTTGATATTTTTATTTG+TGG + chr5_2:3612619-3612638 Msa0750210:intron 15.0%
!! ATTTGATTATGATTATGAAA+TGG + chr5_2:3612779-3612798 Msa0750210:intron 15.0%
!!! TTTTATTTTTCAAGATATAG+TGG - chr5_2:3612813-3612832 None:intergenic 15.0%
!! TAGTTAGTTATTATAAAGTT+TGG + chr5_2:3613126-3613145 Msa0750210:intron 15.0%
!!! TTAATTACTATTTTACACTA+TGG + chr5_2:3613283-3613302 Msa0750210:intron 15.0%
!! TTAAGATTGATGTTATGATA+AGG - chr5_2:3611937-3611956 None:intergenic 20.0%
!! TAAAAGCAATTATAACATGT+GGG - chr5_2:3612506-3612525 None:intergenic 20.0%
!! TTAAAAGCAATTATAACATG+TGG - chr5_2:3612507-3612526 None:intergenic 20.0%
!!! CATTTTATGTAATTAGGTAT+TGG + chr5_2:3612544-3612563 Msa0750210:intron 20.0%
!! ATATCAAAGAATATCCTTAA+AGG - chr5_2:3612576-3612595 None:intergenic 20.0%
!!! TTATTTGTGTTAGGAAATTT+TGG + chr5_2:3612850-3612869 Msa0750210:intron 20.0%
!! AATAACTAACTATTTAGGTA+AGG - chr5_2:3613118-3613137 None:intergenic 20.0%
!! TAAAAATTATAATGCGTCAA+AGG - chr5_2:3613188-3613207 None:intergenic 20.0%
!! GGAATATATTATAAAGCATA+TGG + chr5_2:3613794-3613813 Msa0750210:three_prime_UTR 20.0%
!! ATTACATCATAACATAACAT+AGG - chr5_2:3613853-3613872 None:intergenic 20.0%
! GATGAACGTGTTTATATATA+GGG - chr5_2:3611458-3611477 None:intergenic 25.0%
! TGATGAACGTGTTTATATAT+AGG - chr5_2:3611459-3611478 None:intergenic 25.0%
! TTCGTTTAGAAATACAAAGA+GGG - chr5_2:3611569-3611588 None:intergenic 25.0%
! ATTCGTTTAGAAATACAAAG+AGG - chr5_2:3611570-3611589 None:intergenic 25.0%
! TTTCTAAACGAATAACAAAG+AGG + chr5_2:3611577-3611596 Msa0750210:five_prime_UTR 25.0%
! TTCTAAACGAATAACAAAGA+GGG + chr5_2:3611578-3611597 Msa0750210:five_prime_UTR 25.0%
!!! GGCTCTATTTGATTTTAATT+TGG - chr5_2:3611859-3611878 None:intergenic 25.0%
! AAAATCAAATAGAGCCAATT+AGG + chr5_2:3611863-3611882 Msa0750210:intron 25.0%
! CTAATCAAGCACATAATATT+TGG - chr5_2:3611984-3612003 None:intergenic 25.0%
!! TTATTTGTTTCTCTCTTTGA+AGG + chr5_2:3612007-3612026 Msa0750210:intron 25.0%
! AGAATATGATCATATCAAGT+TGG + chr5_2:3612098-3612117 Msa0750210:intron 25.0%
!! TGGTTTACTATTTTCATACA+TGG + chr5_2:3612304-3612323 Msa0750210:intron 25.0%
!! AAGAATAGATGTTTTCTGTT+TGG + chr5_2:3612415-3612434 Msa0750210:intron 25.0%
! TCAAATACTACATTTGTATG+TGG - chr5_2:3612765-3612784 None:intergenic 25.0%
!! CTTTTTGCATTATTTGTGTT+AGG + chr5_2:3612841-3612860 Msa0750210:intron 25.0%
! AATTCCAATTCAATCAGAAA+TGG - chr5_2:3613405-3613424 None:intergenic 25.0%
!! TAGAAGCATGAAATTGAAAA+GGG - chr5_2:3614087-3614106 None:intergenic 25.0%
! AGAAAAAGTGTGTGTGTTTA+TGG + chr5_2:3611647-3611666 Msa0750210:CDS 30.0%
AATACAAAGTCCTTTGTGTT+AGG - chr5_2:3611746-3611765 None:intergenic 30.0%
TAACACAAAGGACTTTGTAT+TGG + chr5_2:3611745-3611764 Msa0750210:CDS 30.0%
AACACAAAGGACTTTGTATT+GGG + chr5_2:3611746-3611765 Msa0750210:CDS 30.0%
AGATTGATGTTATGATAAGG+AGG - chr5_2:3611934-3611953 None:intergenic 30.0%
TCATGGAGTTTGAATTAAAC+AGG + chr5_2:3612181-3612200 Msa0750210:intron 30.0%
ATTAAACAGGAAGAAAACCT+TGG + chr5_2:3612194-3612213 Msa0750210:intron 30.0%
AAGAAAACCTTGGTAAAATC+TGG + chr5_2:3612204-3612223 Msa0750210:intron 30.0%
TCACATGAATAACTTACTCA+TGG + chr5_2:3612244-3612263 Msa0750210:intron 30.0%
TCATGGAGTTTGAATTAAAC+AGG + chr5_2:3612181-3612200 Msa0750210:intron 30.0%
ATTAAACAGGAAGAAAACCT+TGG + chr5_2:3612194-3612213 Msa0750210:intron 30.0%
AAGAAAACCTTGGTAAAATC+TGG + chr5_2:3612204-3612223 Msa0750210:intron 30.0%
! TAGTAAACCAGATTTTACCA+AGG - chr5_2:3612294-3612313 None:intergenic 30.0%
ACATGGCAAAAAATAGAACA+AGG + chr5_2:3612321-3612340 Msa0750210:intron 30.0%
! ATTTTTCCCGAAATATCTAG+TGG + chr5_2:3612372-3612391 Msa0750210:intron 30.0%
ATCAAAGCCACTAGATATTT+CGG - chr5_2:3612382-3612401 None:intergenic 30.0%
GAAAACATCTATTCTTGCAA+AGG - chr5_2:3612411-3612430 None:intergenic 30.0%
!! TTTTCTGTTTGGCTTCTTAT+TGG + chr5_2:3612426-3612445 Msa0750210:intron 30.0%
! GGTATTGGAATATTCCTTTA+AGG + chr5_2:3612559-3612578 Msa0750210:intron 30.0%
CATAGACAATATCAAAGCTT+CGG + chr5_2:3612922-3612941 Msa0750210:CDS 30.0%
AAAACCACAAATGAATTTCC+AGG + chr5_2:3613061-3613080 Msa0750210:CDS 30.0%
ACTAACTATTTAGGTAAGGT+AGG - chr5_2:3613114-3613133 None:intergenic 30.0%
CGTTATGTCATTGTAAAATG+TGG - chr5_2:3613157-3613176 None:intergenic 30.0%
!!! CTGATGTTGCTTTTGTTTTT+AGG + chr5_2:3613324-3613343 Msa0750210:intron 30.0%
AGTACCATTTCTGATTGAAT+TGG + chr5_2:3613398-3613417 Msa0750210:CDS 30.0%
! TGTTTGCCTGAATTTTATGT+TGG + chr5_2:3613480-3613499 Msa0750210:CDS 30.0%
! GTTTGCCTGAATTTTATGTT+GGG + chr5_2:3613481-3613500 Msa0750210:CDS 30.0%
!! TAGATTTTGAAGGGGAATTT+TGG + chr5_2:3613745-3613764 Msa0750210:three_prime_UTR 30.0%
TTTGACATTCATTTCCCTTT+AGG + chr5_2:3613826-3613845 Msa0750210:three_prime_UTR 30.0%
AACATAACATAGGACCTAAA+GGG - chr5_2:3613843-3613862 None:intergenic 30.0%
TAACATAACATAGGACCTAA+AGG - chr5_2:3613844-3613863 None:intergenic 30.0%
ACACAATAGAAACTACTACT+AGG - chr5_2:3613887-3613906 None:intergenic 30.0%
AAAGCAAGTAAGTTTCACAA+TGG + chr5_2:3613918-3613937 Msa0750210:three_prime_UTR 30.0%
! AGAAGCATGAAATTGAAAAG+GGG - chr5_2:3614086-3614105 None:intergenic 30.0%
! GTAGAAGCATGAAATTGAAA+AGG - chr5_2:3614088-3614107 None:intergenic 30.0%
AAACAAAGTGGTTGAAGGAA+AGG - chr5_2:3611520-3611539 None:intergenic 35.0%
TTTCCTTCAACCACTTTGTT+TGG + chr5_2:3611519-3611538 Msa0750210:five_prime_UTR 35.0%
AGAGAAAATGCCAAACAAAG+TGG - chr5_2:3611532-3611551 None:intergenic 35.0%
GAAATACAAAGAGGGAAGAA+GGG - chr5_2:3611561-3611580 None:intergenic 35.0%
AGAAATACAAAGAGGGAAGA+AGG - chr5_2:3611562-3611581 None:intergenic 35.0%
! TATTGTGTTGTTGAAGAGCA+TGG + chr5_2:3611614-3611633 Msa0750210:exon 35.0%
ATAGAGCCAATTAGGTTTCT+TGG + chr5_2:3611871-3611890 Msa0750210:intron 35.0%
! CTCTTTGAAGGATTTGATCT+TGG + chr5_2:3612019-3612038 Msa0750210:intron 35.0%
CCATGAGTAAGTTATTCATG+TGG - chr5_2:3612167-3612186 None:intergenic 35.0%
CCACATGAATAACTTACTCA+TGG + chr5_2:3612164-3612183 Msa0750210:intron 35.0%
AAACCTTGGTAAAATCTGGT+TGG + chr5_2:3612208-3612227 Msa0750210:intron 35.0%
! AAACCAACCAGATTTTACCA+AGG - chr5_2:3612214-3612233 None:intergenic 35.0%
TCAAAGCCACTAGATATTTC+GGG - chr5_2:3612381-3612400 None:intergenic 35.0%
TCAATTTCGACCCAATCATA+TGG - chr5_2:3612883-3612902 None:intergenic 35.0%
GTGGTGCAATAATGAAAGTT+AGG - chr5_2:3612968-3612987 None:intergenic 35.0%
! TTTTCCATCTAGATGTGCAA+AGG - chr5_2:3613045-3613064 None:intergenic 35.0%
ATATTGATATATGCACCTCC+TGG - chr5_2:3613082-3613101 None:intergenic 35.0%
TGCTGCAAAGAAATCAATGA+AGG + chr5_2:3613545-3613564 Msa0750210:CDS 35.0%
GAAGGAGAAGAAAATGTATC+TGG + chr5_2:3613563-3613582 Msa0750210:CDS 35.0%
TAGATTCACTTGCTTGTGTA+TGG - chr5_2:3613650-3613669 None:intergenic 35.0%
! GAATCTAACATGAGCTTTTC+AGG + chr5_2:3613663-3613682 Msa0750210:CDS 35.0%
TTCTTCAATTCCAATTCCAG+TGG + chr5_2:3613722-3613741 Msa0750210:three_prime_UTR 35.0%
TCAAAATCTACCACTGGAAT+TGG - chr5_2:3613735-3613754 None:intergenic 35.0%
! ATTCCAGTGGTAGATTTTGA+AGG + chr5_2:3613735-3613754 Msa0750210:three_prime_UTR 35.0%
! TTCCAGTGGTAGATTTTGAA+GGG + chr5_2:3613736-3613755 Msa0750210:three_prime_UTR 35.0%
ATTCCTACGTACTAATGAAG+AGG - chr5_2:3613779-3613798 None:intergenic 35.0%
CAATAGAAACTACTACTAGG+TGG - chr5_2:3613884-3613903 None:intergenic 35.0%
! GAATTTGCTTGTGTTGTTGA+AGG + chr5_2:3614032-3614051 Msa0750210:three_prime_UTR 35.0%
ATGCCAAACAAAGTGGTTGA+AGG - chr5_2:3611525-3611544 None:intergenic 40.0%
! TGCTTTGTTCAGACAAGCAT+AGG - chr5_2:3611707-3611726 None:intergenic 40.0%
TGAACAAAGCAATGACTCTG+AGG + chr5_2:3611716-3611735 Msa0750210:CDS 40.0%
TGCATGCCAAGAAACCTAAT+TGG - chr5_2:3611880-3611899 None:intergenic 40.0%
! TCTGTTTGGCTTCTTATTGG+TGG + chr5_2:3612429-3612448 Msa0750210:intron 40.0%
TCGAAATTGAGACAAGAGGT+TGG + chr5_2:3612893-3612912 Msa0750210:CDS 40.0%
ACCACAAATGAATTTCCAGG+AGG + chr5_2:3613064-3613083 Msa0750210:CDS 40.0%
ACCTCCTGGAAATTCATTTG+TGG - chr5_2:3613068-3613087 None:intergenic 40.0%
GCAAACAAGACACAAGTTTC+TGG - chr5_2:3613467-3613486 None:intergenic 40.0%
GGCTTCCCAACATAAAATTC+AGG - chr5_2:3613489-3613508 None:intergenic 40.0%
! AGAAAATGTATCTGGCTCCT+TGG + chr5_2:3613571-3613590 Msa0750210:CDS 40.0%
!! ATGAAACTGCTTTTCCTCCA+AGG - chr5_2:3613591-3613610 None:intergenic 40.0%
GCAGTTTCATGCAAATGAAG+TGG + chr5_2:3613601-3613620 Msa0750210:CDS 40.0%
TTCATGCAAATGAAGTGGTC+AGG + chr5_2:3613606-3613625 Msa0750210:CDS 40.0%
GCTTGTGTATGGTGATATGT+TGG - chr5_2:3613639-3613658 None:intergenic 40.0%
!! TCCAGTGGTAGATTTTGAAG+GGG + chr5_2:3613737-3613756 Msa0750210:three_prime_UTR 40.0%
AGACCTCTTCATTAGTACGT+AGG + chr5_2:3613773-3613792 Msa0750210:three_prime_UTR 40.0%
GCAAGTAAGTTTCACAATGG+AGG + chr5_2:3613921-3613940 Msa0750210:three_prime_UTR 40.0%
TGGAGGAAAGTCGAAAAACT+CGG + chr5_2:3613938-3613957 Msa0750210:three_prime_UTR 40.0%
TCTCTGTTGACAACATCTAG+TGG - chr5_2:3613999-3614018 None:intergenic 40.0%
ATGTTGTCAACAGAGAGTAG+AGG + chr5_2:3614003-3614022 Msa0750210:three_prime_UTR 40.0%
GCATGAAATTGAAAAGGGGA+AGG - chr5_2:3614082-3614101 None:intergenic 40.0%
ACAAGCATAGGTCCTCAAGA+GGG - chr5_2:3611695-3611714 None:intergenic 45.0%
CTGAGGAACACCTAACACAA+AGG + chr5_2:3611733-3611752 Msa0750210:CDS 45.0%
ATCTCAGCTAGCACTTCTTG+AGG + chr5_2:3611770-3611789 Msa0750210:CDS 45.0%
TTGGAGCGACACCATATGAT+TGG + chr5_2:3612869-3612888 Msa0750210:CDS 45.0%
TGGAGCGACACCATATGATT+GGG + chr5_2:3612870-3612889 Msa0750210:CDS 45.0%
TGGGTCGAAATTGAGACAAG+AGG + chr5_2:3612889-3612908 Msa0750210:CDS 45.0%
!! CACTAGTCTTCTCAAACAGG+TGG - chr5_2:3612987-3613006 None:intergenic 45.0%
CACCTGTTTGAGAAGACTAG+TGG + chr5_2:3612985-3613004 Msa0750210:CDS 45.0%
!! AGCCACTAGTCTTCTCAAAC+AGG - chr5_2:3612990-3613009 None:intergenic 45.0%
GCTGCTATGCTATGTGAAAG+AGG + chr5_2:3613007-3613026 Msa0750210:CDS 45.0%
CCATCTAGATGTGCAAAGGT+TGG - chr5_2:3613041-3613060 None:intergenic 45.0%
CCAACCTTTGCACATCTAGA+TGG + chr5_2:3613038-3613057 Msa0750210:CDS 45.0%
CAGTTCCAGATTGCAAAAGC+AGG + chr5_2:3613432-3613451 Msa0750210:CDS 45.0%
!! CTTCACCTGCTTTTGCAATC+TGG - chr5_2:3613440-3613459 None:intergenic 45.0%
ACAATCGCGGTTAAGTACTC+TGG - chr5_2:3613510-3613529 None:intergenic 45.0%
ACACACAAGTCGAACAATCG+CGG - chr5_2:3613523-3613542 None:intergenic 45.0%
! AAATGTATCTGGCTCCTTGG+AGG + chr5_2:3613574-3613593 Msa0750210:CDS 45.0%
TTGACTGCTACCACTGCATT+AGG - chr5_2:3613693-3613712 None:intergenic 45.0%
TCCCCTTCAAAATCTACCAC+TGG - chr5_2:3613741-3613760 None:intergenic 45.0%
AGGAAAGTCGAAAAACTCGG+TGG + chr5_2:3613941-3613960 Msa0750210:three_prime_UTR 45.0%
AGGGGAAGGAAGAATGCAAA+GGG - chr5_2:3614068-3614087 None:intergenic 45.0%
AAGGGGAAGGAAGAATGCAA+AGG - chr5_2:3614069-3614088 None:intergenic 45.0%
!!! TTGATATATATATATATTTT+TGG + chr5_2:3612589-3612608 Msa0750210:intron 5.0%
CAAAGAGGGAAGAAGGGCTA+AGG - chr5_2:3611555-3611574 None:intergenic 50.0%
CAAGCATAGGTCCTCAAGAG+GGG - chr5_2:3611694-3611713 None:intergenic 50.0%
GACAAGCATAGGTCCTCAAG+AGG - chr5_2:3611696-3611715 None:intergenic 50.0%
TTCAGGAGCTCCTAATGCAG+TGG + chr5_2:3613680-3613699 Msa0750210:CDS 50.0%
GAGTCATGTGTCGCGTTTCT+AGG + chr5_2:3613967-3613986 Msa0750210:three_prime_UTR 50.0%
AGAGCTGCATGCCCCTCTTG+AGG + chr5_2:3611680-3611699 Msa0750210:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 3611432 3614155 3611432 ID=Msa0750210;Name=Msa0750210
chr5_2 mRNA 3611432 3614155 3611432 ID=Msa0750210-mRNA-1;Parent=Msa0750210;Name=Msa0750210-mRNA-1;_AED=0.01;_eAED=0.01;_QI=201|1|1|1|1|1|3|439|250
chr5_2 exon 3611432 3611791 3611432 ID=Msa0750210-mRNA-1:exon:1336;Parent=Msa0750210-mRNA-1
chr5_2 exon 3612863 3613085 3612863 ID=Msa0750210-mRNA-1:exon:1337;Parent=Msa0750210-mRNA-1
chr5_2 exon 3613346 3614155 3613346 ID=Msa0750210-mRNA-1:exon:1338;Parent=Msa0750210-mRNA-1
chr5_2 five_prime_UTR 3611432 3611632 3611432 ID=Msa0750210-mRNA-1:five_prime_utr;Parent=Msa0750210-mRNA-1
chr5_2 CDS 3611633 3611791 3611633 ID=Msa0750210-mRNA-1:cds;Parent=Msa0750210-mRNA-1
chr5_2 CDS 3612863 3613085 3612863 ID=Msa0750210-mRNA-1:cds;Parent=Msa0750210-mRNA-1
chr5_2 CDS 3613346 3613716 3613346 ID=Msa0750210-mRNA-1:cds;Parent=Msa0750210-mRNA-1
chr5_2 three_prime_UTR 3613717 3614155 3613717 ID=Msa0750210-mRNA-1:three_prime_utr;Parent=Msa0750210-mRNA-1
Gene Sequence

>Msa0750210

ATGGAAAGCTTTGAAGAAAAAGTGTGTGTGTTTATGGATATTGAATCAGAGCTGCATGCCCCTCTTGAGGACCTATGCTTGTCTGAACAAAGCAATGACTCTGAGGAACACCTAACACAAAGGACTTTGTATTGGGAATCTCAGCTAGCACTTCTTGAGGAAATTTTGGAGCGACACCATATGATTGGGTCGAAATTGAGACAAGAGGTTGGTAGAATCATAGACAATATCAAAGCTTCGGATTATTGCAGCTGCTTCAAGCCTAACTTTCATTATTGCACCACCTGTTTGAGAAGACTAGTGGCTGCTATGCTATGTGAAAGAGGATTCACTACCAACCTTTGCACATCTAGATGGAAAACCACAAATGAATTTCCAGGAGGCTCGCACGAGTACATTGAAGTGATTGCAAGCACAACAACAAGAAAGAAACAAGTACCATTTCTGATTGAATTGGAATTGAAAGAACAGTTCCAGATTGCAAAAGCAGGTGAAGAATACCAGAAACTTGTGTCTTGTTTGCCTGAATTTTATGTTGGGAAGCCAGAGTACTTAACCGCGATTGTTCGACTTGTGTGTGATGCTGCAAAGAAATCAATGAAGGAGAAGAAAATGTATCTGGCTCCTTGGAGGAAAAGCAGTTTCATGCAAATGAAGTGGTCAGGTTTCAATCCAACATATCACCATACACAAGCAAGTGAATCTAACATGAGCTTTTCAGGAGCTCCTAATGCAGTGGTAGCAGTCAACTGA
Protein sequence

>Msa0750210

MESFEEKVCVFMDIESELHAPLEDLCLSEQSNDSEEHLTQRTLYWESQLALLEEILERHHMIGSKLRQEVGRIIDNIKASDYCSCFKPNFHYCTTCLRRLVAAMLCERGFTTNLCTSRWKTTNEFPGGSHEYIEVIASTTTRKKQVPFLIELELKEQFQIAKAGEEYQKLVSCLPEFYVGKPEYLTAIVRLVCDAAKKSMKEKKMYLAPWRKSSFMQMKWSGFNPTYHHTQASESNMSFSGAPNAVVAVN*