AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0794860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794860 MtrunA17_Chr5g0396281 98.400 250 4 0 1 250 1 250 0.0 517
Msa0794860 MtrunA17_Chr2g0282301 35.176 199 125 2 28 222 70 268 1.78e-42 147
Msa0794860 MtrunA17_Chr4g0047231 34.483 232 140 6 12 240 68 290 1.68e-41 144
Msa0794860 MtrunA17_Chr4g0040171 40.331 181 105 3 44 222 66 245 7.78e-41 142
Msa0794860 MtrunA17_Chr4g0047221 32.759 232 143 5 12 240 122 343 1.00e-39 141
Msa0794860 MtrunA17_Chr2g0282311 30.973 226 149 3 18 240 65 286 4.60e-39 138
Msa0794860 MtrunA17_Chr4g0075591 32.673 202 111 6 45 243 79 258 5.61e-30 113
Msa0794860 MtrunA17_Chr4g0075611 32.512 203 113 6 45 243 94 276 1.77e-29 112
Msa0794860 MtrunA17_Chr7g0251511 31.293 147 97 3 94 240 128 270 7.68e-28 108
Msa0794860 MtrunA17_Chr1g0166241 34.677 124 75 1 97 220 153 270 1.73e-24 100
Msa0794860 MtrunA17_Chr2g0289701 34.615 156 84 4 65 220 114 251 2.20e-24 99.4
Msa0794860 MtrunA17_Chr8g0384101 33.594 128 84 1 94 220 79 206 7.52e-24 96.7
Msa0794860 MtrunA17_Chr1g0188091 33.333 141 83 2 97 232 175 309 7.28e-23 95.9
Msa0794860 MtrunA17_Chr1g0184881 34.959 123 74 1 98 220 169 285 2.32e-22 94.0
Msa0794860 MtrunA17_Chr8g0339051 31.544 149 95 3 99 241 133 280 1.29e-21 91.3
Msa0794860 MtrunA17_Chr7g0234691 34.109 129 83 2 95 222 169 296 3.46e-21 90.9
Msa0794860 MtrunA17_Chr7g0260551 32.812 128 78 3 98 225 161 280 1.42e-19 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794860 AT4G32480.1 36.548 197 123 2 28 222 45 241 1.76e-46 156
Msa0794860 AT2G20670.1 35.096 208 130 3 44 249 63 267 2.78e-43 148
Msa0794860 AT3G07350.1 35.714 126 81 0 97 222 135 260 6.89e-30 114
Msa0794860 AT3G25240.1 38.760 129 78 1 95 222 113 241 1.37e-28 110
Msa0794860 AT2G39650.2 33.071 127 85 0 94 220 45 171 1.33e-26 103
Msa0794860 AT2G39650.1 33.071 127 85 0 94 220 107 233 1.97e-26 104
Msa0794860 AT4G14620.1 34.677 124 75 1 97 220 143 260 8.93e-26 103
Msa0794860 AT2G38820.2 36.508 126 73 2 95 220 158 276 1.20e-24 100
Msa0794860 AT1G12030.1 32.558 172 93 5 52 222 81 230 2.19e-23 96.3
Msa0794860 AT3G22970.2 33.871 124 76 1 97 220 57 174 3.89e-23 95.1
Msa0794860 AT3G22970.1 33.871 124 76 1 97 220 168 285 1.76e-22 95.1
Msa0794860 AT1G62420.1 32.540 126 82 1 98 223 109 231 7.98e-22 92.0
Msa0794860 AT3G54550.1 27.465 142 93 2 101 238 148 283 8.92e-17 78.2
Msa0794860 AT1G77145.1 29.545 132 81 2 101 220 79 210 8.74e-15 72.4
Msa0794860 AT1G77160.2 29.545 132 81 2 101 220 29 160 1.15e-14 70.9
Msa0794860 AT1G77160.1 29.545 132 81 2 101 220 84 215 1.56e-14 71.6
Msa0794860 AT1G77145.2 35.789 95 57 1 130 220 59 153 7.51e-14 68.6

Find 84 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTGTTAGGAAATTT+TGG 0.191126 5_3:-6765948 Msa0794860:intron
TGATGAACGTGTTTATATAT+AGG 0.242613 5_3:+6767251 None:intergenic
TTTGACATTCATTTCCCTTT+AGG 0.255256 5_3:-6764964 Msa0794860:three_prime_UTR
GATGAACGTGTTTATATATA+GGG 0.258259 5_3:+6767252 None:intergenic
TATATATAGGGTTTGTTATT+CGG 0.283720 5_3:+6767264 None:intergenic
GCAAACAAGACACAAGTTTC+TGG 0.293899 5_3:+6765326 None:intergenic
TTTCCTTCAACCACTTTGTT+TGG 0.299739 5_3:-6767188 Msa0794860:five_prime_UTR
AGTACCATTTCTGATTGAAT+TGG 0.335912 5_3:-6765392 Msa0794860:CDS
TCAAAATCTACCACTGGAAT+TGG 0.346284 5_3:+6765058 None:intergenic
AACACAAAGGACTTTGTATT+GGG 0.356331 5_3:-6766957 Msa0794860:CDS
AATACAAAGTCCTTTGTGTT+AGG 0.357106 5_3:+6766960 None:intergenic
GAATTTGCTTGTGTTGTTGA+AGG 0.360556 5_3:-6764758 Msa0794860:three_prime_UTR
GAGTCATGTGTCGCGTTTCT+AGG 0.366332 5_3:-6764823 Msa0794860:three_prime_UTR
TTGGAGCGACATCATATGAT+TGG 0.368180 5_3:-6765929 Msa0794860:CDS
CCAACCTTTGCACATCTAGA+TGG 0.378351 5_3:-6765760 Msa0794860:CDS
GGCTTCCCAACATAAAATTC+AGG 0.380679 5_3:+6765304 None:intergenic
TAACACAAAGGACTTTGTAT+TGG 0.383816 5_3:-6766958 Msa0794860:CDS
GAAGGAGAAGAAAATGTATC+TGG 0.388065 5_3:-6765227 Msa0794860:CDS
ATCAGAGCTGCATGCCCCTC+TGG 0.391295 5_3:-6767026 Msa0794860:CDS
AATTCCAATTCAATCAGAAA+TGG 0.405930 5_3:+6765388 None:intergenic
TAGAAGCATGAAATTGAAAA+GGG 0.407876 5_3:+6764706 Msa0794850:intron
GTAGAAGCATGAAATTGAAA+AGG 0.412944 5_3:+6764705 Msa0794850:intron
AAAACCACAAATGAATTTCC+AGG 0.430404 5_3:-6765737 Msa0794860:CDS
TTCATGCAAATGAAGTGGTC+AGG 0.435123 5_3:-6765184 Msa0794860:CDS
GTGGTGCAATAATGAAAGTT+AGG 0.436885 5_3:+6765833 None:intergenic
TTGACTGCTACCACTGCATT+AGG 0.442412 5_3:+6765100 None:intergenic
GGAATATATTATAAAGCATA+TGG 0.454576 5_3:-6764996 Msa0794860:three_prime_UTR
TGGAGCGACATCATATGATT+GGG 0.457776 5_3:-6765928 Msa0794860:CDS
CATAAACAATATCAAAGCTT+CGG 0.461150 5_3:-6765876 Msa0794860:CDS
TAGATTCACTTGCTTGTGTA+TGG 0.461506 5_3:+6765143 None:intergenic
AGAAAGACAAAGAGGGAAGA+AGG 0.470745 5_3:+6767148 None:intergenic
AGCCACTAGTCTTCTCAAAC+AGG 0.471489 5_3:+6765811 None:intergenic
TAACATAACATAGGACCTAA+AGG 0.473739 5_3:+6764949 None:intergenic
ACCTCCTGGAAATTCATTTG+TGG 0.474315 5_3:+6765733 None:intergenic
AGAAAATGTATCTGGCTCCT+TGG 0.483912 5_3:-6765219 Msa0794860:CDS
AACATAACATAGGACCTAAA+GGG 0.488803 5_3:+6764950 None:intergenic
AAATGTATCTGGCTCCTTGG+AGG 0.492634 5_3:-6765216 Msa0794860:CDS
GTGCCAAACAAAGTGGTTGA+AGG 0.509833 5_3:+6767185 None:intergenic
GCTTGTGTATGGTGATATGT+TGG 0.515521 5_3:+6765154 None:intergenic
TCCCCTTCAAAATCTACCAC+TGG 0.525171 5_3:+6765052 None:intergenic
TGCTTTGTTCAGACAAGCAT+AGG 0.542553 5_3:+6766999 None:intergenic
CAAAGAGGGAAGAAGGGCTA+AGG 0.542597 5_3:+6767155 None:intergenic
AAACAAAGTGGTTGAAGGAA+AGG 0.544360 5_3:+6767190 None:intergenic
ACAATCGCGGTTAAGTACTC+TGG 0.547258 5_3:+6765283 None:intergenic
GAAAGACAAAGAGGGAAGAA+GGG 0.549864 5_3:+6767149 None:intergenic
ACACAATAGAAACTACTACT+AGG 0.551480 5_3:+6764906 None:intergenic
CCATCTAGATGTGCAAAGGT+TGG 0.559415 5_3:+6765760 None:intergenic
TGCTGCAAAGAAATCAATGA+AGG 0.567461 5_3:-6765245 Msa0794860:CDS
CAGTTCCAGATTGCAAAAGC+AGG 0.571376 5_3:-6765358 Msa0794860:CDS
GCATGAAATTGAAAAGGGGA+AGG 0.575445 5_3:+6764711 Msa0794850:CDS
AGGGGAAGGAAGAATGCAAA+GGG 0.579820 5_3:+6764725 Msa0794850:CDS
GCAGTTTCATGCAAATGAAG+TGG 0.583111 5_3:-6765189 Msa0794860:CDS
ATTCGTTTAGAAAGACAAAG+AGG 0.584945 5_3:+6767140 None:intergenic
ATTACATCATAACATAACAT+AGG 0.585846 5_3:+6764940 None:intergenic
GCTGCTATGCTATGTGAAAG+AGG 0.587932 5_3:-6765791 Msa0794860:CDS
GACAAGCATAGGTCCTCCAG+AGG 0.591272 5_3:+6767010 None:intergenic
ATTCCTACGTACTAATGAAG+AGG 0.597235 5_3:+6765014 None:intergenic
TTCAGGAGCTCCTAATGCAG+TGG 0.598514 5_3:-6765110 Msa0794860:CDS
GCAAGTAAGTTTCACAATGG+AGG 0.598818 5_3:-6764869 Msa0794860:three_prime_UTR
AAAGCAAGTAAGTTTCACAA+TGG 0.612119 5_3:-6764872 Msa0794860:three_prime_UTR
AGAGCTGCATGCCCCTCTGG+AGG 0.612588 5_3:-6767023 Msa0794860:CDS
GTGTTGTTGAAGAGCATGCA+TGG 0.613418 5_3:-6767089 Msa0794860:exon
TTCTTCAATTCCAATTCCAG+TGG 0.613919 5_3:-6765068 Msa0794860:three_prime_UTR
TTCTAAACGAATAACAAAGA+GGG 0.613986 5_3:-6767129 Msa0794860:five_prime_UTR
TTTCTAAACGAATAACAAAG+AGG 0.615612 5_3:-6767130 Msa0794860:five_prime_UTR
TTCGTTTAGAAAGACAAAGA+GGG 0.620609 5_3:+6767141 None:intergenic
TCGAAATTGAGACAAGAGGT+TGG 0.623564 5_3:-6765905 Msa0794860:CDS
TGGGTCGAAATTGAGACAAG+AGG 0.625191 5_3:-6765909 Msa0794860:CDS
AGAGAAAGTGCCAAACAAAG+TGG 0.639651 5_3:+6767178 None:intergenic
TGAACAAAGCAATGACTCTG+AGG 0.640823 5_3:-6766987 Msa0794860:CDS
CACCTGTTTGAGAAGACTAG+TGG 0.640946 5_3:-6765813 Msa0794860:CDS
AGAAGCATGAAATTGAAAAG+GGG 0.641184 5_3:+6764707 Msa0794850:intron
AAGGGGAAGGAAGAATGCAA+AGG 0.644340 5_3:+6764724 Msa0794850:CDS
CACTAGTCTTCTCAAACAGG+TGG 0.650169 5_3:+6765814 None:intergenic
ATCTCAGCTAGCACTTCTTG+AGG 0.650841 5_3:-6766933 Msa0794860:intron
ACACACAAGTCGAACAATCG+CGG 0.653266 5_3:+6765270 None:intergenic
ATGTTGTCAACAGAGAGTAG+AGG 0.653934 5_3:-6764787 Msa0794860:three_prime_UTR
ACAAGCATAGGTCCTCCAGA+GGG 0.654210 5_3:+6767011 None:intergenic
CAATAGAAACTACTACTAGG+TGG 0.667165 5_3:+6764909 None:intergenic
AGACCTCTTCATTAGTACGT+AGG 0.670566 5_3:-6765017 Msa0794860:three_prime_UTR
TCTCTGTTGACAACATCTAG+TGG 0.677328 5_3:+6764794 Msa0794850:CDS
CTGAGGAACACCTAACACAA+AGG 0.685583 5_3:-6766970 Msa0794860:CDS
CAAGCATAGGTCCTCCAGAG+GGG 0.712265 5_3:+6767012 None:intergenic
ACCACAAATGAATTTCCAGG+AGG 0.741742 5_3:-6765734 Msa0794860:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATTTTATTTTATTT+TGG + chr5_3:6767239-6767258 None:intergenic 0.0%
!!! TAAAATCATTTTATGTAATT+AGG - chr5_3:6765690-6765709 Msa0794860:intron 10.0%
!! TTTATAATAACTAACTATTT+AGG + chr5_3:6766244-6766263 None:intergenic 10.0%
!!! ACAAAATAATGAATTTTTAT+TGG - chr5_3:6766366-6766385 Msa0794860:intron 10.0%
!!! AGTAATTAATTTTAGATTAA+AGG + chr5_3:6766401-6766420 None:intergenic 10.0%
!!! TATTAATTTTTTGACTAAGT+TGG - chr5_3:6765357-6765376 Msa0794860:CDS 15.0%
!!! TTTAAAGAATTCATTTTCAT+TGG + chr5_3:6765499-6765518 None:intergenic 15.0%
!!! TTCTTTGATATTTTTATTTG+TGG - chr5_3:6765737-6765756 Msa0794860:CDS 15.0%
!! ATTTGATTATGATTATGAAA+TGG - chr5_3:6765900-6765919 Msa0794860:CDS 15.0%
!!! TTTTATTTTTCAAGATATAG+TGG + chr5_3:6765934-6765953 None:intergenic 15.0%
!! TAGTTAGTTATTATAAAGTT+TGG - chr5_3:6766247-6766266 Msa0794860:intron 15.0%
!!! TTAATTACTATTTTACACTA+TGG - chr5_3:6766412-6766431 Msa0794860:intron 15.0%
!! TTAATCAAGCACATAATATT+TGG + chr5_3:6765215-6765234 None:intergenic 20.0%
!! TTAAAAGCAATTATAACATG+TGG + chr5_3:6765659-6765678 None:intergenic 20.0%
!!! CATTTTATGTAATTAGGTAT+TGG - chr5_3:6765696-6765715 Msa0794860:intron 20.0%
!! ATATCAAAGAATATCCTTAA+AGG + chr5_3:6765728-6765747 None:intergenic 20.0%
!!! TTATTTGTGTTAGGAAATTT+TGG - chr5_3:6765971-6765990 Msa0794860:intron 20.0%
!! AATAACTAACTATTTAGGTA+AGG + chr5_3:6766239-6766258 None:intergenic 20.0%
!! TAAAAATTATAATGCGTCAA+AGG + chr5_3:6766317-6766336 None:intergenic 20.0%
!! GGAATATATTATAAAGCATA+TGG - chr5_3:6766923-6766942 Msa0794860:intron 20.0%
!! ATTACATCATAACATAACAT+AGG + chr5_3:6766982-6767001 None:intergenic 20.0%
! GATGAACGTGTTTATATATA+GGG + chr5_3:6764670-6764689 Msa0794850:intron 25.0%
! TGATGAACGTGTTTATATAT+AGG + chr5_3:6764671-6764690 Msa0794850:intron 25.0%
! TTTCTAAACGAATAACAAAG+AGG - chr5_3:6764789-6764808 Msa0794860:three_prime_UTR 25.0%
! TTCTAAACGAATAACAAAGA+GGG - chr5_3:6764790-6764809 Msa0794860:three_prime_UTR 25.0%
!!! GGCTCTATTTGATTTTAATT+TGG + chr5_3:6765075-6765094 None:intergenic 25.0%
! AAAATCAAATAGAGCCAATT+AGG - chr5_3:6765079-6765098 Msa0794860:exon 25.0%
! TTAAGCTTGATGTTATGATA+AGG + chr5_3:6765153-6765172 None:intergenic 25.0%
!! TGGTTTACTATTTTCAAACA+TGG - chr5_3:6765453-6765472 Msa0794860:intron 25.0%
! ATGGCAAAAAATAGAACAAA+AGG - chr5_3:6765472-6765491 Msa0794860:intron 25.0%
!! AAGAATAGATGTTTTCTGTT+TGG - chr5_3:6765565-6765584 Msa0794860:intron 25.0%
! AATACTACTACATTTCTATG+TGG + chr5_3:6765883-6765902 None:intergenic 25.0%
! CTTATTGCATTATTTGTGTT+AGG - chr5_3:6765962-6765981 Msa0794860:intron 25.0%
! CATAAACAATATCAAAGCTT+CGG - chr5_3:6766043-6766062 Msa0794860:intron 25.0%
! AGTTATGTCATTGTAAAATG+TGG + chr5_3:6766278-6766297 None:intergenic 25.0%
! AATTCCAATTCAATCAGAAA+TGG + chr5_3:6766534-6766553 None:intergenic 25.0%
!! TAGAAGCATGAAATTGAAAA+GGG + chr5_3:6767216-6767235 None:intergenic 25.0%
TTCGTTTAGAAAGACAAAGA+GGG + chr5_3:6764781-6764800 Msa0794850:CDS 30.0%
ATTCGTTTAGAAAGACAAAG+AGG + chr5_3:6764782-6764801 Msa0794850:CDS 30.0%
! AGAAAAAGTGTGTGTGTTTA+TGG - chr5_3:6764863-6764882 Msa0794860:three_prime_UTR 30.0%
AATACAAAGTCCTTTGTGTT+AGG + chr5_3:6764962-6764981 None:intergenic 30.0%
TAACACAAAGGACTTTGTAT+TGG - chr5_3:6764961-6764980 Msa0794860:three_prime_UTR 30.0%
AACACAAAGGACTTTGTATT+GGG - chr5_3:6764962-6764981 Msa0794860:three_prime_UTR 30.0%
! TTATTTGTTTCTCCCTTTGA+AGG - chr5_3:6765238-6765257 Msa0794860:CDS 30.0%
AGAATATGATCACATCAAGT+TGG - chr5_3:6765327-6765346 Msa0794860:CDS 30.0%
TCACATGAATAACTTACTCA+TGG - chr5_3:6765393-6765412 Msa0794860:CDS 30.0%
TCATGGAGTTTGAATTAAAC+AGG - chr5_3:6765410-6765429 Msa0794860:CDS 30.0%
ATTAAACAGGAAGAAAACCT+TGG - chr5_3:6765423-6765442 Msa0794860:CDS 30.0%
AAGAAAACCTTGGTAAAATC+TGG - chr5_3:6765433-6765452 Msa0794860:CDS 30.0%
! TAGTAAACCAGATTTTACCA+AGG + chr5_3:6765443-6765462 None:intergenic 30.0%
! ATTTTTCCCGAAATATCTAG+TGG - chr5_3:6765523-6765542 Msa0794860:intron 30.0%
ATCAAAGCCACTAGATATTT+CGG + chr5_3:6765533-6765552 None:intergenic 30.0%
AGAAAACATCTATTCTTGCA+AGG + chr5_3:6765562-6765581 None:intergenic 30.0%
!! TTCTGTTTGGCTTCTTTTAT+TGG - chr5_3:6765578-6765597 Msa0794860:intron 30.0%
! GGTATTGGAATATTCCTTTA+AGG - chr5_3:6765711-6765730 Msa0794860:intron 30.0%
AAAACCACAAATGAATTTCC+AGG - chr5_3:6766182-6766201 Msa0794860:intron 30.0%
ACTAACTATTTAGGTAAGGT+AGG + chr5_3:6766235-6766254 None:intergenic 30.0%
!!! CTGATGTTGCTTTTGTTTTT+AGG - chr5_3:6766453-6766472 Msa0794860:intron 30.0%
AGTACCATTTCTGATTGAAT+TGG - chr5_3:6766527-6766546 Msa0794860:intron 30.0%
! TGTTTGCCTGAATTTTATGT+TGG - chr5_3:6766609-6766628 Msa0794860:intron 30.0%
! GTTTGCCTGAATTTTATGTT+GGG - chr5_3:6766610-6766629 Msa0794860:intron 30.0%
!! TAGATTTTGAAGGGGAATTT+TGG - chr5_3:6766874-6766893 Msa0794860:intron 30.0%
TTTGACATTCATTTCCCTTT+AGG - chr5_3:6766955-6766974 Msa0794860:CDS 30.0%
AACATAACATAGGACCTAAA+GGG + chr5_3:6766972-6766991 None:intergenic 30.0%
TAACATAACATAGGACCTAA+AGG + chr5_3:6766973-6766992 None:intergenic 30.0%
ACACAATAGAAACTACTACT+AGG + chr5_3:6767016-6767035 None:intergenic 30.0%
AAAGCAAGTAAGTTTCACAA+TGG - chr5_3:6767047-6767066 Msa0794860:CDS 30.0%
! AGAAGCATGAAATTGAAAAG+GGG + chr5_3:6767215-6767234 None:intergenic 30.0%
! GTAGAAGCATGAAATTGAAA+AGG + chr5_3:6767217-6767236 None:intergenic 30.0%
AAACAAAGTGGTTGAAGGAA+AGG + chr5_3:6764732-6764751 Msa0794850:CDS 35.0%
TTTCCTTCAACCACTTTGTT+TGG - chr5_3:6764731-6764750 Msa0794860:three_prime_UTR 35.0%
ATAGAGCCAATTAGGTTTCT+TGG - chr5_3:6765087-6765106 Msa0794860:exon 35.0%
AGCTTGATGTTATGATAAGG+AGG + chr5_3:6765150-6765169 None:intergenic 35.0%
CCAAGATCAAATCCTTCAAA+GGG + chr5_3:6765253-6765272 None:intergenic 35.0%
TCAAAGCCACTAGATATTTC+GGG + chr5_3:6765532-6765551 None:intergenic 35.0%
!! TGTTTGGCTTCTTTTATTGG+TGG - chr5_3:6765581-6765600 Msa0794860:intron 35.0%
GTGGTGCAATAATGAAAGTT+AGG + chr5_3:6766089-6766108 None:intergenic 35.0%
! TTTTCCATCTAGATGTGCAA+AGG + chr5_3:6766166-6766185 None:intergenic 35.0%
ATATTGATATATGCACCTCC+TGG + chr5_3:6766203-6766222 None:intergenic 35.0%
TGCTGCAAAGAAATCAATGA+AGG - chr5_3:6766674-6766693 Msa0794860:intron 35.0%
GAAGGAGAAGAAAATGTATC+TGG - chr5_3:6766692-6766711 Msa0794860:intron 35.0%
TAGATTCACTTGCTTGTGTA+TGG + chr5_3:6766779-6766798 None:intergenic 35.0%
! GAATCTAACATGAGCTTTTC+AGG - chr5_3:6766792-6766811 Msa0794860:intron 35.0%
TTCTTCAATTCCAATTCCAG+TGG - chr5_3:6766851-6766870 Msa0794860:intron 35.0%
TCAAAATCTACCACTGGAAT+TGG + chr5_3:6766864-6766883 None:intergenic 35.0%
! ATTCCAGTGGTAGATTTTGA+AGG - chr5_3:6766864-6766883 Msa0794860:intron 35.0%
! TTCCAGTGGTAGATTTTGAA+GGG - chr5_3:6766865-6766884 Msa0794860:intron 35.0%
ATTCCTACGTACTAATGAAG+AGG + chr5_3:6766908-6766927 None:intergenic 35.0%
CAATAGAAACTACTACTAGG+TGG + chr5_3:6767013-6767032 None:intergenic 35.0%
! GAATTTGCTTGTGTTGTTGA+AGG - chr5_3:6767161-6767180 Msa0794860:five_prime_UTR 35.0%
!! AGAGAAAGTGCCAAACAAAG+TGG + chr5_3:6764744-6764763 Msa0794850:CDS 40.0%
GAAAGACAAAGAGGGAAGAA+GGG + chr5_3:6764773-6764792 Msa0794850:CDS 40.0%
AGAAAGACAAAGAGGGAAGA+AGG + chr5_3:6764774-6764793 Msa0794850:CDS 40.0%
! TGCTTTGTTCAGACAAGCAT+AGG + chr5_3:6764923-6764942 None:intergenic 40.0%
TGAACAAAGCAATGACTCTG+AGG - chr5_3:6764932-6764951 Msa0794860:three_prime_UTR 40.0%
TGCATGCCAAGAAACCTAAT+TGG + chr5_3:6765096-6765115 None:intergenic 40.0%
! CCCTTTGAAGGATTTGATCT+TGG - chr5_3:6765250-6765269 Msa0794860:CDS 40.0%
GCCAAGATCAAATCCTTCAA+AGG + chr5_3:6765254-6765273 None:intergenic 40.0%
TTGGAGCGACATCATATGAT+TGG - chr5_3:6765990-6766009 Msa0794860:intron 40.0%
TGGAGCGACATCATATGATT+GGG - chr5_3:6765991-6766010 Msa0794860:intron 40.0%
TCGAAATTGAGACAAGAGGT+TGG - chr5_3:6766014-6766033 Msa0794860:intron 40.0%
ACCACAAATGAATTTCCAGG+AGG - chr5_3:6766185-6766204 Msa0794860:intron 40.0%
ACCTCCTGGAAATTCATTTG+TGG + chr5_3:6766189-6766208 None:intergenic 40.0%
GCAAACAAGACACAAGTTTC+TGG + chr5_3:6766596-6766615 None:intergenic 40.0%
GGCTTCCCAACATAAAATTC+AGG + chr5_3:6766618-6766637 None:intergenic 40.0%
! AGAAAATGTATCTGGCTCCT+TGG - chr5_3:6766700-6766719 Msa0794860:intron 40.0%
!! ATGAAACTGCTTTTCCTCCA+AGG + chr5_3:6766720-6766739 None:intergenic 40.0%
GCAGTTTCATGCAAATGAAG+TGG - chr5_3:6766730-6766749 Msa0794860:intron 40.0%
TTCATGCAAATGAAGTGGTC+AGG - chr5_3:6766735-6766754 Msa0794860:intron 40.0%
GCTTGTGTATGGTGATATGT+TGG + chr5_3:6766768-6766787 None:intergenic 40.0%
!! TCCAGTGGTAGATTTTGAAG+GGG - chr5_3:6766866-6766885 Msa0794860:intron 40.0%
AGACCTCTTCATTAGTACGT+AGG - chr5_3:6766902-6766921 Msa0794860:intron 40.0%
GCAAGTAAGTTTCACAATGG+AGG - chr5_3:6767050-6767069 Msa0794860:CDS 40.0%
TGGAGGAAAGTCGAAAAACT+CGG - chr5_3:6767067-6767086 Msa0794860:CDS 40.0%
TCTCTGTTGACAACATCTAG+TGG + chr5_3:6767128-6767147 None:intergenic 40.0%
ATGTTGTCAACAGAGAGTAG+AGG - chr5_3:6767132-6767151 Msa0794860:five_prime_UTR 40.0%
GCATGAAATTGAAAAGGGGA+AGG + chr5_3:6767211-6767230 None:intergenic 40.0%
GTGCCAAACAAAGTGGTTGA+AGG + chr5_3:6764737-6764756 Msa0794850:CDS 45.0%
! GTGTTGTTGAAGAGCATGCA+TGG - chr5_3:6764830-6764849 Msa0794860:three_prime_UTR 45.0%
CTGAGGAACACCTAACACAA+AGG - chr5_3:6764949-6764968 Msa0794860:three_prime_UTR 45.0%
ATCTCAGCTAGCACTTCTTG+AGG - chr5_3:6764986-6765005 Msa0794860:three_prime_UTR 45.0%
TGGGTCGAAATTGAGACAAG+AGG - chr5_3:6766010-6766029 Msa0794860:intron 45.0%
!! CACTAGTCTTCTCAAACAGG+TGG + chr5_3:6766108-6766127 None:intergenic 45.0%
CACCTGTTTGAGAAGACTAG+TGG - chr5_3:6766106-6766125 Msa0794860:intron 45.0%
!! AGCCACTAGTCTTCTCAAAC+AGG + chr5_3:6766111-6766130 None:intergenic 45.0%
GCTGCTATGCTATGTGAAAG+AGG - chr5_3:6766128-6766147 Msa0794860:intron 45.0%
CCATCTAGATGTGCAAAGGT+TGG + chr5_3:6766162-6766181 None:intergenic 45.0%
CCAACCTTTGCACATCTAGA+TGG - chr5_3:6766159-6766178 Msa0794860:intron 45.0%
CAGTTCCAGATTGCAAAAGC+AGG - chr5_3:6766561-6766580 Msa0794860:intron 45.0%
!! CTTCACCTGCTTTTGCAATC+TGG + chr5_3:6766569-6766588 None:intergenic 45.0%
ACAATCGCGGTTAAGTACTC+TGG + chr5_3:6766639-6766658 None:intergenic 45.0%
ACACACAAGTCGAACAATCG+CGG + chr5_3:6766652-6766671 None:intergenic 45.0%
! AAATGTATCTGGCTCCTTGG+AGG - chr5_3:6766703-6766722 Msa0794860:intron 45.0%
TTGACTGCTACCACTGCATT+AGG + chr5_3:6766822-6766841 None:intergenic 45.0%
TCCCCTTCAAAATCTACCAC+TGG + chr5_3:6766870-6766889 None:intergenic 45.0%
AGGAAAGTCGAAAAACTCGG+TGG - chr5_3:6767070-6767089 Msa0794860:CDS 45.0%
AGGGGAAGGAAGAATGCAAA+GGG + chr5_3:6767197-6767216 None:intergenic 45.0%
AAGGGGAAGGAAGAATGCAA+AGG + chr5_3:6767198-6767217 None:intergenic 45.0%
CAAAGAGGGAAGAAGGGCTA+AGG + chr5_3:6764767-6764786 Msa0794850:CDS 50.0%
ACAAGCATAGGTCCTCCAGA+GGG + chr5_3:6764911-6764930 None:intergenic 50.0%
TTCAGGAGCTCCTAATGCAG+TGG - chr5_3:6766809-6766828 Msa0794860:intron 50.0%
GAGTCATGTGTCGCGTTTCT+AGG - chr5_3:6767096-6767115 Msa0794860:five_prime_UTR 50.0%
CAAGCATAGGTCCTCCAGAG+GGG + chr5_3:6764910-6764929 None:intergenic 55.0%
GACAAGCATAGGTCCTCCAG+AGG + chr5_3:6764912-6764931 None:intergenic 55.0%
ATCAGAGCTGCATGCCCCTC+TGG - chr5_3:6764893-6764912 Msa0794860:three_prime_UTR 60.0%
AGAGCTGCATGCCCCTCTGG+AGG - chr5_3:6764896-6764915 Msa0794860:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 6764657 6767284 6764657 ID=Msa0794860;Name=Msa0794860
chr5_3 mRNA 6764657 6767284 6764657 ID=Msa0794860-mRNA-1;Parent=Msa0794860;Name=Msa0794860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=192|1|1|1|1|1|3|439|250
chr5_3 exon 6764657 6765466 6764657 ID=Msa0794860-mRNA-1:exon:2757;Parent=Msa0794860-mRNA-1
chr5_3 exon 6765735 6765957 6765735 ID=Msa0794860-mRNA-1:exon:2756;Parent=Msa0794860-mRNA-1
chr5_3 exon 6766934 6767284 6766934 ID=Msa0794860-mRNA-1:exon:2755;Parent=Msa0794860-mRNA-1
chr5_3 five_prime_UTR 6767093 6767284 6767093 ID=Msa0794860-mRNA-1:five_prime_utr;Parent=Msa0794860-mRNA-1
chr5_3 CDS 6766934 6767092 6766934 ID=Msa0794860-mRNA-1:cds;Parent=Msa0794860-mRNA-1
chr5_3 CDS 6765735 6765957 6765735 ID=Msa0794860-mRNA-1:cds;Parent=Msa0794860-mRNA-1
chr5_3 CDS 6765096 6765466 6765096 ID=Msa0794860-mRNA-1:cds;Parent=Msa0794860-mRNA-1
chr5_3 three_prime_UTR 6764657 6765095 6764657 ID=Msa0794860-mRNA-1:three_prime_utr;Parent=Msa0794860-mRNA-1
Gene Sequence

>Msa0794860

ATGGAAAGCTTTGAAGAAAAAGTGTGTGTGTTTATGGATATTGAATCAGAGCTGCATGCCCCTCTGGAGGACCTATGCTTGTCTGAACAAAGCAATGACTCTGAGGAACACCTAACACAAAGGACTTTGTATTGGGAATCTCAGCTAGCACTTCTTGAGGAAATTTTGGAGCGACATCATATGATTGGGTCGAAATTGAGACAAGAGGTTGGTAGAATCATAAACAATATCAAAGCTTCGGATTATTGCAGCTGCTTCAAGCCTAACTTTCATTATTGCACCACCTGTTTGAGAAGACTAGTGGCTGCTATGCTATGTGAAAGAGGATTCACTACCAACCTTTGCACATCTAGATGGAAAACCACAAATGAATTTCCAGGAGGCTCGCACGAGTACATTGAAGTGATTGCAAGCACAACAACAAGAAAGAAACAAGTACCATTTCTGATTGAATTGGAATTGAAAGAACAGTTCCAGATTGCAAAAGCAGGTGAAGAATACCAGAAACTTGTGTCTTGTTTGCCTGAATTTTATGTTGGGAAGCCAGAGTACTTAACCGCGATTGTTCGACTTGTGTGTGATGCTGCAAAGAAATCAATGAAGGAGAAGAAAATGTATCTGGCTCCTTGGAGGAAAAGCAGTTTCATGCAAATGAAGTGGTCAGGTTTCAATCCAACATATCACCATACACAAGCAAGTGAATCTAACATGAGCTTTTCAGGAGCTCCTAATGCAGTGGTAGCAGTCAACTGA
Protein sequence

>Msa0794860

MESFEEKVCVFMDIESELHAPLEDLCLSEQSNDSEEHLTQRTLYWESQLALLEEILERHHMIGSKLRQEVGRIINNIKASDYCSCFKPNFHYCTTCLRRLVAAMLCERGFTTNLCTSRWKTTNEFPGGSHEYIEVIASTTTRKKQVPFLIELELKEQFQIAKAGEEYQKLVSCLPEFYVGKPEYLTAIVRLVCDAAKKSMKEKKMYLAPWRKSSFMQMKWSGFNPTYHHTQASESNMSFSGAPNAVVAVN*