AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0752660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752660 MtrunA17_Chr5g0396771 91.566 83 7 0 5 87 9 91 1.55e-51 156
Msa0752660 MtrunA17_Chr4g0024181 45.833 72 39 0 9 80 2 73 9.40e-19 73.2
Msa0752660 MtrunA17_Chr2g0322831 54.839 62 24 1 17 78 20 77 1.93e-18 72.4
Msa0752660 MtrunA17_Chr2g0288361 40.789 76 42 1 5 80 2 74 1.42e-15 65.1
Msa0752660 MtrunA17_Chr4g0055981 56.410 39 17 0 35 73 113 151 2.76e-11 57.0
Msa0752660 MtrunA17_Chr8g0360111 56.410 39 17 0 35 73 83 121 2.85e-11 57.4
Msa0752660 MtrunA17_Chr4g0070641 55.000 40 18 0 34 73 69 108 5.53e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752660 AT2G30420.1 63.415 82 24 1 6 87 17 92 1.15e-29 102
Msa0752660 AT5G53200.1 70.312 64 19 0 24 87 22 85 1.64e-29 101
Msa0752660 AT2G46410.1 60.811 74 29 0 13 86 16 89 1.89e-28 98.6
Msa0752660 AT2G30424.1 59.740 77 30 1 12 87 12 88 4.68e-27 95.1
Msa0752660 AT2G30432.1 65.152 66 23 0 19 84 19 84 9.89e-27 94.0
Msa0752660 AT4G01060.1 55.844 77 34 0 1 77 1 77 1.13e-25 90.9
Msa0752660 AT4G01060.2 63.492 63 23 0 15 77 12 74 5.11e-25 89.4
Msa0752660 AT1G01380.1 75.556 45 11 0 33 77 32 76 1.61e-20 78.2
Msa0752660 AT4G01060.3 69.565 46 14 0 32 77 30 75 1.37e-19 75.5
Msa0752660 AT5G52600.1 58.974 39 16 0 35 73 70 108 9.59e-12 58.2

Find 24 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCTTCACTTCTTTCTTT+TGG 0.236926 5_2:+6401967 None:intergenic
CGGAGGTTTGACTTGTGTTT+CGG 0.239671 5_2:+6402921 None:intergenic
CAGAAGAAATAGAGAGATTC+TGG 0.240435 5_2:-6402018 Msa0752660:CDS
GGAGGTTTGACTTGTGTTTC+GGG 0.242590 5_2:+6402922 None:intergenic
GGGAGATATTTGCCTTAGTT+TGG 0.291933 5_2:+6402942 None:intergenic
ACCACCAGGTAGATAAAATA+TGG 0.317616 5_2:-6402975 None:intergenic
TTGATAGCAGGTCGCATTCC+GGG 0.323951 5_2:-6402049 Msa0752660:CDS
GGAGATATTTGCCTTAGTTT+GGG 0.373654 5_2:+6402943 None:intergenic
TTTGATAGCAGGTCGCATTC+CGG 0.383477 5_2:-6402050 Msa0752660:CDS
CAATTATATCTCTCTCTCCT+AGG 0.480221 5_2:-6401910 Msa0752660:three_prime_UTR
GGAAGAACGTTCCCAAACTA+AGG 0.483455 5_2:-6402954 Msa0752660:CDS
TGTACGACCTAGTTGGAGAT+AGG 0.497484 5_2:-6402622 Msa0752660:intron
CAGAAGTGAGCAGCAATGAG+TGG 0.497509 5_2:-6402691 Msa0752660:CDS
CGCAGGATGTACGACCTAGT+TGG 0.536525 5_2:-6402629 Msa0752660:CDS
AACAGGTGGAATTTGATAGC+AGG 0.543963 5_2:-6402061 Msa0752660:intron
ATTTCTTCTGCTTTACGACC+CGG 0.559635 5_2:+6402031 None:intergenic
AAGATAAAGCAAATTAAACT+AGG 0.566688 5_2:-6401951 Msa0752660:three_prime_UTR
CATAGAGATGAGCGAGCAAG+AGG 0.568119 5_2:-6402663 Msa0752660:CDS
AAGAGGAGGATCTCATTCGC+AGG 0.581290 5_2:-6402646 Msa0752660:CDS
AGAAGTGAGCAGCAATGAGT+GGG 0.602111 5_2:-6402690 Msa0752660:CDS
AGAGATGAGCGAGCAAGAGG+AGG 0.612798 5_2:-6402660 Msa0752660:CDS
AGATTAGAAACCACCTTCGG+AGG 0.612968 5_2:+6402904 None:intergenic
AATATTACCTATCTCCAACT+AGG 0.621481 5_2:+6402615 None:intergenic
AACACAAGTCAAACCTCCGA+AGG 0.653483 5_2:-6402917 Msa0752660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGCAATAGAGAAAATAA+TGG + chr5_2:6402035-6402054 None:intergenic 20.0%
!! AAACATTAAATACTAGTATG+AGG + chr5_2:6402142-6402161 None:intergenic 20.0%
!!! TATCTTTTGCAAAATCATAA+TGG + chr5_2:6402434-6402453 None:intergenic 20.0%
!!! TCTAATTAGTTCTAATAACT+TGG + chr5_2:6402552-6402571 None:intergenic 20.0%
!! ATTAGAACTAATTAGAAACA+CGG - chr5_2:6402556-6402575 Msa0752660:intron 20.0%
!!! AAAGTTGGTCAATATTTTAA+AGG + chr5_2:6402681-6402700 None:intergenic 20.0%
!! AAGATAAAGCAAATTAAACT+AGG - chr5_2:6402927-6402946 Msa0752660:CDS 20.0%
!! AAAAGCAATAGAGAAAATAA+TGG + chr5_2:6402035-6402054 None:intergenic 20.0%
!! AAACATTAAATACTAGTATG+AGG + chr5_2:6402142-6402161 None:intergenic 20.0%
!!! TATCTTTTGCAAAATCATAA+TGG + chr5_2:6402434-6402453 None:intergenic 20.0%
!!! TCTAATTAGTTCTAATAACT+TGG + chr5_2:6402552-6402571 None:intergenic 20.0%
!! ATTAGAACTAATTAGAAACA+CGG - chr5_2:6402556-6402575 Msa0752660:intron 20.0%
!!! AAAGTTGGTCAATATTTTAA+AGG + chr5_2:6402681-6402700 None:intergenic 20.0%
!! AAGATAAAGCAAATTAAACT+AGG - chr5_2:6402927-6402946 Msa0752660:CDS 20.0%
!!! TTTTAAGCTTTGACGTTTTA+AGG - chr5_2:6402328-6402347 Msa0752660:intron 25.0%
! TATGATACTAATTGCATGTT+TGG + chr5_2:6402519-6402538 None:intergenic 25.0%
! TTACTAAAGATGTGTTTGAT+AGG - chr5_2:6402613-6402632 Msa0752660:intron 25.0%
!! TTTTTATGCTGTATGATGTT+TGG - chr5_2:6402698-6402717 Msa0752660:CDS 25.0%
!! TTATCTTCACTTCTTTCTTT+TGG + chr5_2:6402914-6402933 None:intergenic 25.0%
!!! TTTTAAGCTTTGACGTTTTA+AGG - chr5_2:6402328-6402347 Msa0752660:intron 25.0%
! TATGATACTAATTGCATGTT+TGG + chr5_2:6402519-6402538 None:intergenic 25.0%
! TTACTAAAGATGTGTTTGAT+AGG - chr5_2:6402613-6402632 Msa0752660:intron 25.0%
!! TTTTTATGCTGTATGATGTT+TGG - chr5_2:6402698-6402717 Msa0752660:CDS 25.0%
!! TTATCTTCACTTCTTTCTTT+TGG + chr5_2:6402914-6402933 None:intergenic 25.0%
AGAGAAAATAATGGCATGTT+GGG + chr5_2:6402026-6402045 None:intergenic 30.0%
TAGAGAAAATAATGGCATGT+TGG + chr5_2:6402027-6402046 None:intergenic 30.0%
TTATACGTGTACCAATTAAG+AGG - chr5_2:6402084-6402103 Msa0752660:intron 30.0%
AATATTACCTATCTCCAACT+AGG + chr5_2:6402266-6402285 None:intergenic 30.0%
! TATATTCCTTTGACGTTTTG+AGG - chr5_2:6402294-6402313 Msa0752660:intron 30.0%
AAAAAACCTCAAAACGTCAA+AGG + chr5_2:6402303-6402322 None:intergenic 30.0%
TACAATTACAAAGAAGCTGA+GGG - chr5_2:6402384-6402403 Msa0752660:intron 30.0%
! TAATTGCATGTTTGGTAACA+TGG + chr5_2:6402511-6402530 None:intergenic 30.0%
! GTTCTAATAACTTGGTCTTT+CGG + chr5_2:6402544-6402563 None:intergenic 30.0%
TAATTAGAAACACGGTGAAA+AGG - chr5_2:6402564-6402583 Msa0752660:intron 30.0%
CATCATACAGCATAAAAAGT+TGG + chr5_2:6402696-6402715 None:intergenic 30.0%
! GTCATAAGCTTTTACATGAA+AGG - chr5_2:6402765-6402784 Msa0752660:intron 30.0%
AATTATATCTCTCTCTCCTA+GGG - chr5_2:6402969-6402988 Msa0752660:exon 30.0%
AGAGAAAATAATGGCATGTT+GGG + chr5_2:6402026-6402045 None:intergenic 30.0%
TAGAGAAAATAATGGCATGT+TGG + chr5_2:6402027-6402046 None:intergenic 30.0%
TTATACGTGTACCAATTAAG+AGG - chr5_2:6402084-6402103 Msa0752660:intron 30.0%
AATATTACCTATCTCCAACT+AGG + chr5_2:6402266-6402285 None:intergenic 30.0%
! TATATTCCTTTGACGTTTTG+AGG - chr5_2:6402294-6402313 Msa0752660:intron 30.0%
AAAAAACCTCAAAACGTCAA+AGG + chr5_2:6402303-6402322 None:intergenic 30.0%
TACAATTACAAAGAAGCTGA+GGG - chr5_2:6402384-6402403 Msa0752660:intron 30.0%
! TAATTGCATGTTTGGTAACA+TGG + chr5_2:6402511-6402530 None:intergenic 30.0%
! GTTCTAATAACTTGGTCTTT+CGG + chr5_2:6402544-6402563 None:intergenic 30.0%
TAATTAGAAACACGGTGAAA+AGG - chr5_2:6402564-6402583 Msa0752660:intron 30.0%
CATCATACAGCATAAAAAGT+TGG + chr5_2:6402696-6402715 None:intergenic 30.0%
! GTCATAAGCTTTTACATGAA+AGG - chr5_2:6402765-6402784 Msa0752660:intron 30.0%
GGAGATATTTGCCTTAGTTT+GGG + chr5_2:6401938-6401957 None:intergenic 35.0%
AGAAAATAATGGCATGTTGG+GGG + chr5_2:6402024-6402043 None:intergenic 35.0%
GAGAAAATAATGGCATGTTG+GGG + chr5_2:6402025-6402044 None:intergenic 35.0%
ATGCATCATGTCCTCTTAAT+TGG + chr5_2:6402098-6402117 None:intergenic 35.0%
CTACAATTACAAAGAAGCTG+AGG - chr5_2:6402383-6402402 Msa0752660:intron 35.0%
TTATGCTGTATGATGTTTGG+TGG - chr5_2:6402701-6402720 Msa0752660:intron 35.0%
AAGAAACTACACAATGCAAC+AGG - chr5_2:6402800-6402819 Msa0752660:intron 35.0%
CAGAAGAAATAGAGAGATTC+TGG - chr5_2:6402860-6402879 Msa0752660:intron 35.0%
! TCTTCACTTCTTTCTTTTGG+CGG + chr5_2:6402911-6402930 None:intergenic 35.0%
CAATTATATCTCTCTCTCCT+AGG - chr5_2:6402968-6402987 Msa0752660:exon 35.0%
ATTATATCTCTCTCTCCTAG+GGG - chr5_2:6402970-6402989 Msa0752660:exon 35.0%
GGAGATATTTGCCTTAGTTT+GGG + chr5_2:6401938-6401957 None:intergenic 35.0%
AGAAAATAATGGCATGTTGG+GGG + chr5_2:6402024-6402043 None:intergenic 35.0%
GAGAAAATAATGGCATGTTG+GGG + chr5_2:6402025-6402044 None:intergenic 35.0%
ATGCATCATGTCCTCTTAAT+TGG + chr5_2:6402098-6402117 None:intergenic 35.0%
CTACAATTACAAAGAAGCTG+AGG - chr5_2:6402383-6402402 Msa0752660:intron 35.0%
TTATGCTGTATGATGTTTGG+TGG - chr5_2:6402701-6402720 Msa0752660:intron 35.0%
AAGAAACTACACAATGCAAC+AGG - chr5_2:6402800-6402819 Msa0752660:intron 35.0%
CAGAAGAAATAGAGAGATTC+TGG - chr5_2:6402860-6402879 Msa0752660:intron 35.0%
! TCTTCACTTCTTTCTTTTGG+CGG + chr5_2:6402911-6402930 None:intergenic 35.0%
GGGAGATATTTGCCTTAGTT+TGG + chr5_2:6401939-6401958 None:intergenic 40.0%
GGTAGATTAGAAACCACCTT+CGG + chr5_2:6401980-6401999 None:intergenic 40.0%
AAACTACACAATGCAACAGG+TGG - chr5_2:6402803-6402822 Msa0752660:intron 40.0%
! AACAGGTGGAATTTGATAGC+AGG - chr5_2:6402817-6402836 Msa0752660:intron 40.0%
ATTTCTTCTGCTTTACGACC+CGG + chr5_2:6402850-6402869 None:intergenic 40.0%
GGGAGATATTTGCCTTAGTT+TGG + chr5_2:6401939-6401958 None:intergenic 40.0%
GGTAGATTAGAAACCACCTT+CGG + chr5_2:6401980-6401999 None:intergenic 40.0%
AAACTACACAATGCAACAGG+TGG - chr5_2:6402803-6402822 Msa0752660:intron 40.0%
! AACAGGTGGAATTTGATAGC+AGG - chr5_2:6402817-6402836 Msa0752660:intron 40.0%
ATTTCTTCTGCTTTACGACC+CGG + chr5_2:6402850-6402869 None:intergenic 40.0%
GGAAGAACGTTCCCAAACTA+AGG - chr5_2:6401924-6401943 Msa0752660:three_prime_UTR 45.0%
!! GGAGGTTTGACTTGTGTTTC+GGG + chr5_2:6401959-6401978 None:intergenic 45.0%
!! CGGAGGTTTGACTTGTGTTT+CGG + chr5_2:6401960-6401979 None:intergenic 45.0%
AACACAAGTCAAACCTCCGA+AGG - chr5_2:6401961-6401980 Msa0752660:exon 45.0%
AGATTAGAAACCACCTTCGG+AGG + chr5_2:6401977-6401996 None:intergenic 45.0%
AATAATGGCATGTTGGGGGT+GGG + chr5_2:6402020-6402039 None:intergenic 45.0%
AAATAATGGCATGTTGGGGG+TGG + chr5_2:6402021-6402040 None:intergenic 45.0%
AGAAGTGAGCAGCAATGAGT+GGG - chr5_2:6402188-6402207 Msa0752660:intron 45.0%
TGTACGACCTAGTTGGAGAT+AGG - chr5_2:6402256-6402275 Msa0752660:intron 45.0%
! TTTGATAGCAGGTCGCATTC+CGG - chr5_2:6402828-6402847 Msa0752660:intron 45.0%
GGAAGAACGTTCCCAAACTA+AGG - chr5_2:6401924-6401943 Msa0752660:three_prime_UTR 45.0%
!! GGAGGTTTGACTTGTGTTTC+GGG + chr5_2:6401959-6401978 None:intergenic 45.0%
!! CGGAGGTTTGACTTGTGTTT+CGG + chr5_2:6401960-6401979 None:intergenic 45.0%
AACACAAGTCAAACCTCCGA+AGG - chr5_2:6401961-6401980 Msa0752660:exon 45.0%
AGATTAGAAACCACCTTCGG+AGG + chr5_2:6401977-6401996 None:intergenic 45.0%
AATAATGGCATGTTGGGGGT+GGG + chr5_2:6402020-6402039 None:intergenic 45.0%
AAATAATGGCATGTTGGGGG+TGG + chr5_2:6402021-6402040 None:intergenic 45.0%
AGAAGTGAGCAGCAATGAGT+GGG - chr5_2:6402188-6402207 Msa0752660:intron 45.0%
TGTACGACCTAGTTGGAGAT+AGG - chr5_2:6402256-6402275 Msa0752660:intron 45.0%
! TTTGATAGCAGGTCGCATTC+CGG - chr5_2:6402828-6402847 Msa0752660:intron 45.0%
ACAAGTCAAACCTCCGAAGG+TGG - chr5_2:6401964-6401983 Msa0752660:exon 50.0%
CAGAAGTGAGCAGCAATGAG+TGG - chr5_2:6402187-6402206 Msa0752660:intron 50.0%
CATAGAGATGAGCGAGCAAG+AGG - chr5_2:6402215-6402234 Msa0752660:intron 50.0%
AAGAGGAGGATCTCATTCGC+AGG - chr5_2:6402232-6402251 Msa0752660:intron 50.0%
! TTGATAGCAGGTCGCATTCC+GGG - chr5_2:6402829-6402848 Msa0752660:intron 50.0%
ACAAGTCAAACCTCCGAAGG+TGG - chr5_2:6401964-6401983 Msa0752660:exon 50.0%
CAGAAGTGAGCAGCAATGAG+TGG - chr5_2:6402187-6402206 Msa0752660:intron 50.0%
CATAGAGATGAGCGAGCAAG+AGG - chr5_2:6402215-6402234 Msa0752660:intron 50.0%
AAGAGGAGGATCTCATTCGC+AGG - chr5_2:6402232-6402251 Msa0752660:intron 50.0%
! TTGATAGCAGGTCGCATTCC+GGG - chr5_2:6402829-6402848 Msa0752660:intron 50.0%
TGGGTGTGTGTGTGTGTTGG+GGG + chr5_2:6402001-6402020 None:intergenic 55.0%
GTGGGTGTGTGTGTGTGTTG+GGG + chr5_2:6402002-6402021 None:intergenic 55.0%
GGTGGGTGTGTGTGTGTGTT+GGG + chr5_2:6402003-6402022 None:intergenic 55.0%
AGAGATGAGCGAGCAAGAGG+AGG - chr5_2:6402218-6402237 Msa0752660:intron 55.0%
CGCAGGATGTACGACCTAGT+TGG - chr5_2:6402249-6402268 Msa0752660:intron 55.0%
TGGGTGTGTGTGTGTGTTGG+GGG + chr5_2:6402001-6402020 None:intergenic 55.0%
GTGGGTGTGTGTGTGTGTTG+GGG + chr5_2:6402002-6402021 None:intergenic 55.0%
GGTGGGTGTGTGTGTGTGTT+GGG + chr5_2:6402003-6402022 None:intergenic 55.0%
AGAGATGAGCGAGCAAGAGG+AGG - chr5_2:6402218-6402237 Msa0752660:intron 55.0%
CGCAGGATGTACGACCTAGT+TGG - chr5_2:6402249-6402268 Msa0752660:intron 55.0%
GGGTGGGTGTGTGTGTGTGT+TGG + chr5_2:6402004-6402023 None:intergenic 60.0%
GGGTGGGTGTGTGTGTGTGT+TGG + chr5_2:6402004-6402023 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 6401910 6402990 6401910 ID=Msa0752660;Name=Msa0752660
chr5_2 mRNA 6401910 6402990 6401910 ID=Msa0752660-mRNA-1;Parent=Msa0752660;Name=Msa0752660-mRNA-1;_AED=0.01;_eAED=0.01;_QI=12|0.5|0.66|1|0.5|0.33|3|63|87
chr5_2 exon 6401910 6402078 6401910 ID=Msa0752660-mRNA-1:exon:2724;Parent=Msa0752660-mRNA-1
chr5_2 exon 6402623 6402719 6402623 ID=Msa0752660-mRNA-1:exon:2723;Parent=Msa0752660-mRNA-1
chr5_2 exon 6402918 6402990 6402918 ID=Msa0752660-mRNA-1:exon:2722;Parent=Msa0752660-mRNA-1
chr5_2 five_prime_UTR 6402979 6402990 6402979 ID=Msa0752660-mRNA-1:five_prime_utr;Parent=Msa0752660-mRNA-1
chr5_2 CDS 6402918 6402978 6402918 ID=Msa0752660-mRNA-1:cds;Parent=Msa0752660-mRNA-1
chr5_2 CDS 6402623 6402719 6402623 ID=Msa0752660-mRNA-1:cds;Parent=Msa0752660-mRNA-1
chr5_2 CDS 6401973 6402078 6401973 ID=Msa0752660-mRNA-1:cds;Parent=Msa0752660-mRNA-1
chr5_2 three_prime_UTR 6401910 6401972 6401910 ID=Msa0752660-mRNA-1:three_prime_utr;Parent=Msa0752660-mRNA-1
Gene Sequence

>Msa0752660

ATGGAAGAACGTTCCCAAACTAAGGCAAATATCTCCCGAAACACAAGTCAAACCTCCGAAGCTGATGCAGAAGTGAGCAGCAATGAGTGGGAGTTCATAGAGATGAGCGAGCAAGAGGAGGATCTCATTCGCAGGATGTACGACCTAGTTGGAGATAGGTGGAATTTGATAGCAGGTCGCATTCCGGGTCGTAAAGCAGAAGAAATAGAGAGATTCTGGATTATGAAACACGCTGATGCTTTTTCCGCCAAAAGAAAGAAGTGA

Protein sequence

>Msa0752660

MEERSQTKANISRNTSQTSEADAEVSSNEWEFIEMSEQEEDLIRRMYDLVGDRWNLIAGRIPGRKAEEIERFWIMKHADAFSAKRKK*