AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0753640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753640 MtrunA17_Chr6g0467061 60.302 199 33 1 1 153 10 208 1.98e-75 231
Msa0753640 MtrunA17_Chr6g0467061 52.632 190 44 1 10 153 88 277 1.68e-59 190
Msa0753640 MtrunA17_Chr6g0467061 50.838 179 42 2 10 142 157 335 2.43e-46 156
Msa0753640 MtrunA17_Chr2g0316611 55.276 199 43 1 1 153 27 225 1.39e-67 212
Msa0753640 MtrunA17_Chr2g0316611 51.579 190 46 1 10 153 174 363 1.32e-57 186
Msa0753640 MtrunA17_Chr2g0316611 53.191 188 42 2 12 153 107 294 6.55e-57 184
Msa0753640 MtrunA17_Chr2g0316611 67.742 62 20 0 10 71 312 373 7.37e-21 89.7
Msa0753640 MtrunA17_Chr2g0316631 55.276 199 43 1 1 153 27 225 1.42e-67 210
Msa0753640 MtrunA17_Chr2g0316631 51.596 188 45 1 12 153 107 294 1.38e-57 184
Msa0753640 MtrunA17_Chr2g0316631 66.129 62 21 0 10 71 243 304 1.49e-19 85.1
Msa0753640 MtrunA17_Chr5g0398011 66.667 141 24 1 8 148 33 150 1.36e-58 182
Msa0753640 MtrunA17_Chr5g0398011 71.154 52 15 0 102 153 35 86 1.81e-18 79.0
Msa0753640 MtrunA17_Chr8g0377221 51.370 146 47 2 9 153 4 126 6.12e-40 138
Msa0753640 MtrunA17_Chr8g0377221 32.381 210 115 5 10 204 74 271 6.26e-25 99.0
Msa0753640 MtrunA17_Chr8g0377221 64.000 50 18 0 100 149 3 52 2.50e-13 67.4
Msa0753640 MtrunA17_Chr8g0382971 47.682 151 79 0 3 153 18 168 9.80e-39 137
Msa0753640 MtrunA17_Chr8g0382971 36.691 139 88 0 12 150 142 280 1.06e-20 89.0
Msa0753640 MtrunA17_Chr8g0382971 35.664 143 92 0 11 153 72 214 2.47e-20 87.8
Msa0753640 MtrunA17_Chr8g0380791 49.645 141 71 0 3 143 6 146 5.26e-38 140
Msa0753640 MtrunA17_Chr8g0380791 38.298 141 86 1 9 149 58 197 2.63e-18 83.2
Msa0753640 MtrunA17_Chr8g0380791 63.462 52 19 0 102 153 13 64 7.66e-16 76.3
Msa0753640 MtrunA17_Chr8g0380791 35.417 96 62 0 7 102 102 197 6.21e-11 61.6
Msa0753640 MtrunA17_Chr8g0377211 46.667 150 79 1 4 153 14 162 2.53e-36 125
Msa0753640 MtrunA17_Chr5g0398021 51.200 125 38 1 12 136 15 116 2.44e-35 122
Msa0753640 MtrunA17_Chr5g0398021 66.667 60 20 0 94 153 5 64 1.15e-22 90.1
Msa0753640 MtrunA17_Chr8g0385241 45.517 145 74 1 1 140 1 145 3.94e-32 121
Msa0753640 MtrunA17_Chr8g0385241 36.416 173 106 2 10 181 204 373 2.82e-22 94.0
Msa0753640 MtrunA17_Chr8g0385241 68.182 44 14 0 106 149 14 57 6.54e-12 64.3
Msa0753640 MtrunA17_Chr8g0377671 43.624 149 84 0 5 153 15 163 5.23e-32 118
Msa0753640 MtrunA17_Chr8g0377671 33.540 161 84 1 9 146 111 271 1.46e-23 95.9
Msa0753640 MtrunA17_Chr4g0059911 40.397 151 89 1 4 153 3 153 8.71e-32 119
Msa0753640 MtrunA17_Chr4g0059911 35.032 157 79 1 10 143 56 212 1.19e-20 89.0
Msa0753640 MtrunA17_Chr4g0059911 38.136 118 73 0 12 129 104 221 1.66e-18 83.2
Msa0753640 MtrunA17_Chr4g0059911 53.448 58 27 0 95 152 2 59 2.79e-11 62.4
Msa0753640 MtrunA17_Chr4g0059911 36.893 103 64 1 24 125 162 264 7.36e-11 60.8
Msa0753640 MtrunA17_Chr8g0377191 41.830 153 89 0 1 153 3 155 5.74e-31 113
Msa0753640 MtrunA17_Chr5g0424301 38.298 141 87 0 7 147 66 206 3.88e-28 111
Msa0753640 MtrunA17_Chr5g0424301 30.994 171 113 2 12 181 94 260 8.28e-18 81.6
Msa0753640 MtrunA17_Chr1g0171491 39.716 141 85 0 7 147 58 198 1.17e-27 110
Msa0753640 MtrunA17_Chr1g0171491 34.722 144 93 1 1 143 74 217 7.76e-18 82.0
Msa0753640 MtrunA17_Chr1g0171491 30.986 142 74 3 11 152 131 248 5.12e-12 64.7
Msa0753640 MtrunA17_Chr3g0115111 39.041 146 88 1 3 147 50 195 5.22e-27 108
Msa0753640 MtrunA17_Chr3g0115111 31.690 142 97 0 12 153 83 224 5.66e-18 82.4
Msa0753640 MtrunA17_Chr3g0115111 30.769 143 93 2 12 154 129 265 3.92e-11 62.4
Msa0753640 MtrunA17_Chr3g0115111 35.294 85 55 0 7 91 147 231 4.73e-11 62.0
Msa0753640 MtrunA17_Chr8g0385201 38.356 146 89 1 3 147 23 168 1.65e-25 103
Msa0753640 MtrunA17_Chr8g0385201 36.691 139 88 0 12 150 56 194 7.05e-20 87.8
Msa0753640 MtrunA17_Chr7g0256301 37.821 156 96 1 7 161 68 223 1.66e-24 101
Msa0753640 MtrunA17_Chr7g0256301 31.818 132 90 0 12 143 96 227 1.48e-14 72.4
Msa0753640 MtrunA17_Chr7g0256301 32.000 125 85 0 9 133 116 240 1.40e-11 63.5
Msa0753640 MtrunA17_Chr3g0131001 41.135 141 83 0 7 147 52 192 4.97e-24 99.8
Msa0753640 MtrunA17_Chr3g0082501 37.589 141 88 0 7 147 61 201 1.05e-23 99.0
Msa0753640 MtrunA17_Chr3g0082501 36.806 144 90 1 9 152 109 251 3.42e-20 88.6
Msa0753640 MtrunA17_Chr3g0082501 34.028 144 94 1 12 155 89 231 1.60e-16 78.2
Msa0753640 MtrunA17_Chr8g0353121 37.589 141 88 0 7 147 50 190 5.22e-23 97.1
Msa0753640 MtrunA17_Chr8g0353121 36.111 144 91 1 12 155 78 220 1.06e-18 84.3
Msa0753640 MtrunA17_Chr8g0353121 36.879 141 88 1 9 149 98 237 4.41e-18 82.4
Msa0753640 MtrunA17_Chr1g0180261 35.762 151 97 0 3 153 44 194 8.53e-23 96.3
Msa0753640 MtrunA17_Chr8g0377201 44.792 96 39 1 79 174 4 85 9.41e-20 80.5
Msa0753640 MtrunA17_Chr8g0377201 63.462 52 18 1 6 57 23 73 6.28e-14 65.1
Msa0753640 MtrunA17_Chr8g0383331 38.525 122 75 0 11 132 28 149 1.57e-18 79.7
Msa0753640 MtrunA17_Chr8g0383551 31.694 183 118 4 6 184 138 317 1.16e-16 77.8
Msa0753640 MtrunA17_Chr8g0383551 49.367 79 34 2 92 166 14 90 4.14e-14 70.5
Msa0753640 MtrunA17_Chr8g0336161 31.847 157 103 1 4 160 237 389 3.58e-15 73.9
Msa0753640 MtrunA17_Chr8g0336161 29.012 162 92 1 5 143 192 353 8.93e-13 67.0
Msa0753640 MtrunA17_Chr7g0233351 31.288 163 85 2 6 143 188 348 9.10e-15 72.8
Msa0753640 MtrunA17_Chr7g0233351 32.090 134 91 0 4 137 232 365 4.13e-13 67.8
Msa0753640 MtrunA17_Chr4g0055371 28.485 165 95 1 6 147 168 332 1.62e-14 72.0
Msa0753640 MtrunA17_Chr4g0055371 32.836 134 90 0 4 137 212 345 4.20e-13 67.8
Msa0753640 MtrunA17_Chr3g0114791 29.814 161 90 2 6 143 181 341 2.18e-14 71.6
Msa0753640 MtrunA17_Chr5g0424111 29.630 162 91 1 5 143 192 353 4.28e-14 70.9
Msa0753640 MtrunA17_Chr5g0424111 32.090 134 91 0 4 137 237 370 6.35e-13 67.4
Msa0753640 MtrunA17_Chr3g0085921 40.952 105 59 2 103 207 11 112 9.10e-13 62.8
Msa0753640 MtrunA17_Chr1g0210831 30.882 136 92 1 2 137 607 740 2.12e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753640 AT1G21980.1 41.026 156 91 1 7 161 73 228 1.45e-32 124
Msa0753640 AT1G21980.1 34.848 132 86 0 12 143 101 232 3.97e-18 83.2
Msa0753640 AT1G77740.2 39.161 143 87 0 7 149 74 216 1.94e-28 113
Msa0753640 AT1G77740.2 33.099 142 95 0 12 153 102 243 6.16e-19 85.5
Msa0753640 AT1G77740.1 39.161 143 87 0 7 149 74 216 1.94e-28 113
Msa0753640 AT1G77740.1 33.099 142 95 0 12 153 102 243 6.16e-19 85.5
Msa0753640 AT2G41210.1 39.007 141 86 0 7 147 67 207 6.99e-27 108
Msa0753640 AT2G41210.1 35.606 132 85 0 12 143 95 226 8.98e-19 85.1
Msa0753640 AT2G41210.1 32.540 126 79 2 6 128 158 280 5.61e-12 65.1
Msa0753640 AT2G41210.1 31.858 113 77 0 10 122 139 251 9.43e-11 61.6
Msa0753640 AT3G07960.3 39.007 141 86 0 7 147 24 164 3.19e-26 106
Msa0753640 AT3G07960.2 39.007 141 86 0 7 147 24 164 3.33e-26 106
Msa0753640 AT3G07960.4 39.007 141 86 0 7 147 24 164 3.33e-26 106
Msa0753640 AT1G60890.2 39.860 143 84 1 1 143 16 156 4.91e-26 106
Msa0753640 AT1G60890.2 34.028 144 94 1 1 143 36 179 2.10e-18 84.0
Msa0753640 AT1G60890.2 30.000 140 74 2 10 149 92 207 6.59e-13 67.8
Msa0753640 AT1G60890.2 51.923 52 25 0 102 153 23 74 9.63e-11 61.6
Msa0753640 AT3G07960.1 39.007 141 86 0 7 147 24 164 6.31e-26 105
Msa0753640 AT1G60890.1 39.416 137 83 0 7 143 8 144 7.38e-26 105
Msa0753640 AT1G60890.1 34.028 144 94 1 1 143 24 167 2.30e-18 84.0
Msa0753640 AT1G60890.1 30.000 140 74 2 10 149 80 195 6.21e-13 67.8
Msa0753640 AT1G60890.1 51.923 52 25 0 102 153 11 62 9.42e-11 61.6
Msa0753640 AT3G56960.2 37.589 141 88 0 7 147 69 209 1.64e-25 104
Msa0753640 AT3G56960.2 34.848 132 86 0 12 143 97 228 1.55e-18 84.3
Msa0753640 AT3G56960.2 28.495 186 121 4 10 195 141 314 4.77e-11 62.4
Msa0753640 AT3G56960.1 37.589 141 88 0 7 147 69 209 1.64e-25 104
Msa0753640 AT3G56960.1 34.848 132 86 0 12 143 97 228 1.55e-18 84.3
Msa0753640 AT3G56960.1 28.495 186 121 4 10 195 141 314 4.77e-11 62.4
Msa0753640 AT2G26420.2 36.646 161 101 1 3 162 46 206 2.82e-25 103
Msa0753640 AT2G26420.3 36.646 161 101 1 3 162 46 206 2.82e-25 103
Msa0753640 AT2G26420.1 36.646 161 101 1 3 162 46 206 3.05e-25 103
Msa0753640 AT1G10900.2 40.741 135 80 0 9 143 10 144 8.94e-25 102
Msa0753640 AT1G10900.2 33.333 144 95 1 1 143 24 167 8.71e-16 76.3
Msa0753640 AT1G10900.1 40.741 135 80 0 9 143 10 144 8.94e-25 102
Msa0753640 AT1G10900.1 33.333 144 95 1 1 143 24 167 8.71e-16 76.3
Msa0753640 AT1G10900.3 40.741 135 80 0 9 143 10 144 8.94e-25 102
Msa0753640 AT1G10900.3 33.333 144 95 1 1 143 24 167 8.71e-16 76.3
Msa0753640 AT3G09920.3 37.255 153 94 1 3 153 44 196 2.82e-24 100
Msa0753640 AT3G09920.3 31.783 129 65 1 15 143 127 232 1.10e-12 67.4
Msa0753640 AT3G09920.4 37.255 153 94 1 3 153 44 196 2.82e-24 100
Msa0753640 AT3G09920.4 31.783 129 65 1 15 143 127 232 1.10e-12 67.4
Msa0753640 AT3G09920.2 37.255 153 94 1 3 153 44 196 2.82e-24 100
Msa0753640 AT3G09920.2 31.783 129 65 1 15 143 127 232 1.10e-12 67.4
Msa0753640 AT3G09920.1 37.255 153 94 1 3 153 44 196 2.82e-24 100
Msa0753640 AT3G09920.1 31.783 129 65 1 15 143 127 232 1.10e-12 67.4
Msa0753640 AT4G17080.1 30.435 161 89 1 6 143 253 413 7.57e-15 73.6
Msa0753640 AT4G17080.1 31.126 151 104 0 6 156 299 449 5.11e-14 70.9
Msa0753640 AT2G35170.1 29.193 161 91 1 6 143 222 382 2.90e-14 71.6
Msa0753640 AT2G35170.1 32.836 134 90 0 4 137 266 399 3.34e-14 71.6
Msa0753640 AT1G77660.1 33.835 133 88 0 6 138 220 352 1.88e-13 69.3
Msa0753640 AT1G77660.1 32.609 138 93 0 6 143 197 334 3.42e-13 68.6
Msa0753640 AT1G21920.2 33.088 136 90 1 8 143 196 330 1.61e-12 66.2
Msa0753640 AT1G21920.2 30.303 132 92 0 6 137 216 347 5.42e-11 62.0
Msa0753640 AT1G21920.1 33.088 136 90 1 8 143 196 330 1.97e-12 66.2
Msa0753640 AT1G21920.1 30.303 132 92 0 6 137 216 347 5.34e-11 62.0
Msa0753640 AT1G75010.1 28.676 136 95 1 2 137 596 729 5.96e-11 62.0

Find 75 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCATCAGAAGCAAGTTT+TGG 0.286127 5_2:+7284093 Msa0753640:CDS
GAGAAATATATGAGGGTGAT+TGG 0.301706 5_2:+7284034 Msa0753640:CDS
TATGAAGGCGATTGGGTTGA+TGG 0.333358 5_2:+7284248 Msa0753640:CDS
TATGAGGGTGATTGGGTTGA+TGG 0.335707 5_2:+7284042 Msa0753640:CDS
TACGAGGGTGATTGGGTTGA+TGG 0.337164 5_2:+7283766 Msa0753640:CDS
TACGAGAATGATCTTTGTTA+TGG 0.368721 5_2:+7283916 Msa0753640:CDS
GAACAATATACGAGGGTGAT+TGG 0.386941 5_2:+7283758 Msa0753640:CDS
TGGGTTGATGGAAGCAGGAC+AGG 0.432064 5_2:+7284054 Msa0753640:CDS
ACACCTTTATAAAATCCAAT+GGG 0.441320 5_2:+7283944 Msa0753640:CDS
AACAATATACGAGGGTGATT+GGG 0.451603 5_2:+7283759 Msa0753640:CDS
AGAAATATATGAGGGTGATT+GGG 0.454244 5_2:+7284035 Msa0753640:CDS
GAACAATATATGAAGGCGAT+TGG 0.460789 5_2:+7284240 Msa0753640:CDS
CCCTCAAAACTTGTTCCTGA+TGG 0.466807 5_2:-7284302 None:intergenic
GGAAAGTACACATGGTCAAA+TGG 0.467614 5_2:+7284218 Msa0753640:CDS
GGAAAGTTCACATGGTCAAA+TGG 0.472329 5_2:+7283736 Msa0753640:CDS
CCCTCAAAACTTGCTCCTGA+TGG 0.474150 5_2:-7283820 None:intergenic
TGGGTTGATGGAGAAAGGAC+AGG 0.477380 5_2:+7283778 Msa0753640:CDS
ATGTGTACTTTCCCTTTCCA+TGG 0.477629 5_2:-7284002 Msa0753650:CDS
ACACCTTTACAGAATCCAAT+GGG 0.479362 5_2:+7283875 Msa0753640:CDS
AAAGGTGTCGTGACCATGAC+GGG 0.483261 5_2:-7283860 None:intergenic
AAGGATGTCGTGACCATGAC+GGG 0.483261 5_2:-7284135 Msa0753650:CDS
ACCCCAAAACTTGCTTCTGA+TGG 0.484460 5_2:-7284096 Msa0753650:CDS
TGGGTTGATGGAAGAAGGAC+GGG 0.487631 5_2:+7284260 Msa0753640:CDS
TTCTTTAGGGATTCCCGTCA+TGG 0.490423 5_2:+7283847 Msa0753640:CDS
AACAATATATGAAGGCGATT+GGG 0.493162 5_2:+7284241 Msa0753640:CDS
GTGATTGGGTTGATGGAGAA+AGG 0.501414 5_2:+7283773 Msa0753640:CDS
GTCAGAAGGAGAAATATATG+AGG 0.504038 5_2:+7284026 Msa0753640:CDS
CATCGTTTCTTGTAATGCCT+AGG 0.506493 5_2:+7283641 None:intergenic
GGACGAATCATATATCCATC+AGG 0.508082 5_2:+7284287 Msa0753640:CDS
GAGTAGTTCTTTCCATGGCA+AGG 0.509436 5_2:+7284196 Msa0753640:CDS
GATGGAAGAAGGACGGGGAA+AGG 0.509452 5_2:+7284266 Msa0753640:CDS
GTGATTGGGTTGATGGAAGC+AGG 0.510508 5_2:+7284049 Msa0753640:CDS
TTCAAGAGTAGTTCTTTCCA+TGG 0.519621 5_2:+7284191 Msa0753640:CDS
GCGATTGGGTTGATGGAAGA+AGG 0.523501 5_2:+7284255 Msa0753640:CDS
AAATACTTCACTTTAAACCT+AGG 0.523798 5_2:-7283658 None:intergenic
GATGGAGAAAGGACAGGGAA+AGG 0.525808 5_2:+7283784 Msa0753640:CDS
GGGCAGATCATATATCCATC+AGG 0.526445 5_2:+7283805 Msa0753640:CDS
ACTTTCCCTTGCCATGCCTA+CGG 0.535916 5_2:-7283720 None:intergenic
ATGTGTACTTTCCCTTGCCA+TGG 0.537930 5_2:-7284208 None:intergenic
TACAAGGGAAACCGTAGGCA+TGG 0.541675 5_2:+7283709 Msa0753640:CDS
ATGGAGAAAGGACAGGGAAA+GGG 0.542724 5_2:+7283785 Msa0753640:CDS
ATCCCCATTGGATTCTGTAA+AGG 0.549491 5_2:-7283878 None:intergenic
CTCTCTGGGAATTCCCGTCA+TGG 0.550940 5_2:+7284122 Msa0753640:CDS
TCAAATGGAACAATATATGA+AGG 0.553404 5_2:+7284233 Msa0753640:CDS
ACATCCTTACAAAATCTGAC+GGG 0.557167 5_2:+7284150 Msa0753640:CDS
GATGGAAGCAGGACAGGGAA+AGG 0.558142 5_2:+7284060 Msa0753640:CDS
TCCAATGGGGATGTCTACAT+TGG 0.559068 5_2:+7283958 Msa0753640:CDS
GACACCTTTATAAAATCCAA+TGG 0.560778 5_2:+7283943 Msa0753640:CDS
AGTAGTTCTTTCCATGGCAA+GGG 0.568168 5_2:+7284197 Msa0753640:CDS
GGAAAGTACACATGGTCAGA+AGG 0.570552 5_2:+7284012 Msa0753640:CDS
ACCAATGTAGACATCCCCAT+TGG 0.574302 5_2:-7283890 None:intergenic
ACCAATGTAGACATCCCCAT+TGG 0.574302 5_2:-7283959 None:intergenic
GGGAAACCGTAGGCATGGCA+AGG 0.580919 5_2:+7283714 Msa0753640:CDS
TCCAATGGGGATGTCTACAT+TGG 0.587195 5_2:+7283889 Msa0753640:CDS
GACATCCTTACAAAATCTGA+CGG 0.587276 5_2:+7284149 Msa0753640:CDS
CCAAGTGGGAATGTCTACAT+CGG 0.602894 5_2:+7283682 Msa0753640:CDS
TTGGGTTGATGGAAGAAGGA+CGG 0.605111 5_2:+7284259 Msa0753640:CDS
GGGTTGATGGAGAAAGGACA+GGG 0.609767 5_2:+7283779 Msa0753640:CDS
CCGATGTAGACATTCCCACT+TGG 0.616297 5_2:-7283682 None:intergenic
TCTGACGGGGATATCTACAT+TGG 0.616887 5_2:+7284164 Msa0753640:CDS
GACACCTTTACAGAATCCAA+TGG 0.624890 5_2:+7283874 Msa0753640:CDS
GGGTTGATGGAAGAAGGACG+GGG 0.626871 5_2:+7284261 Msa0753640:CDS
TCAGAAGGAGAAATATATGA+GGG 0.627968 5_2:+7284027 Msa0753640:CDS
GGGTTGATGGAAGCAGGACA+GGG 0.629912 5_2:+7284055 Msa0753640:CDS
TAAAGGTGTCGTGACCATGA+CGG 0.631292 5_2:-7283861 None:intergenic
TAAGGATGTCGTGACCATGA+CGG 0.637553 5_2:-7284136 Msa0753650:CDS
GGAAACCGTAGGCATGGCAA+GGG 0.656718 5_2:+7283715 Msa0753640:CDS
CATCCTTACAAAATCTGACG+GGG 0.676948 5_2:+7284151 Msa0753640:CDS
ATGGCAAGGGAAAGTTCACA+TGG 0.680438 5_2:+7283728 Msa0753640:CDS
CACCTTTATAAAATCCAATG+GGG 0.689593 5_2:+7283945 Msa0753640:CDS
ATGGCAAGGGAAAGTACACA+TGG 0.692208 5_2:+7284210 Msa0753640:CDS
GTCAAATGGAACAATATACG+AGG 0.694597 5_2:+7283750 Msa0753640:CDS
ATGGAAAGGGAAAGTACACA+TGG 0.695936 5_2:+7284004 Msa0753640:CDS
TCAAATGGAACAATATACGA+GGG 0.706226 5_2:+7283751 Msa0753640:CDS
CACCTTTACAGAATCCAATG+GGG 0.774096 5_2:+7283876 Msa0753640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATACTTCACTTTAAACCT+AGG - chr5_2:7283661-7283680 None:intergenic 25.0%
! ACACCTTTATAAAATCCAAT+GGG + chr5_2:7283944-7283963 Msa0753640:CDS 25.0%
! TCAAATGGAACAATATATGA+AGG + chr5_2:7284233-7284252 Msa0753640:CDS 25.0%
!! AAAGTGAAGTATTTTCCAAG+TGG + chr5_2:7283667-7283686 Msa0753640:CDS 30.0%
!! AAGTGAAGTATTTTCCAAGT+GGG + chr5_2:7283668-7283687 Msa0753640:CDS 30.0%
TCAAATGGAACAATATACGA+GGG + chr5_2:7283751-7283770 Msa0753640:CDS 30.0%
!! TTTTGAGGGTGAATTCTTTA+GGG + chr5_2:7283834-7283853 Msa0753640:CDS 30.0%
TACGAGAATGATCTTTGTTA+TGG + chr5_2:7283916-7283935 Msa0753640:CDS 30.0%
GACACCTTTATAAAATCCAA+TGG + chr5_2:7283943-7283962 Msa0753640:CDS 30.0%
CACCTTTATAAAATCCAATG+GGG + chr5_2:7283945-7283964 Msa0753640:CDS 30.0%
! ATCCCCATTGGATTTTATAA+AGG - chr5_2:7283950-7283969 None:intergenic 30.0%
! TTTGAGAATGATCTTTTCCA+TGG + chr5_2:7283985-7284004 Msa0753640:CDS 30.0%
! AATGATCTTTTCCATGGAAA+GGG + chr5_2:7283991-7284010 Msa0753640:CDS 30.0%
TCAGAAGGAGAAATATATGA+GGG + chr5_2:7284027-7284046 Msa0753640:CDS 30.0%
AGAAATATATGAGGGTGATT+GGG + chr5_2:7284035-7284054 Msa0753640:CDS 30.0%
AACAATATATGAAGGCGATT+GGG + chr5_2:7284241-7284260 Msa0753640:CDS 30.0%
!!! TGTCTACATCGGTTTTTACA+AGG + chr5_2:7283693-7283712 Msa0753640:CDS 35.0%
!!! GTCTACATCGGTTTTTACAA+GGG + chr5_2:7283694-7283713 Msa0753640:CDS 35.0%
GTCAAATGGAACAATATACG+AGG + chr5_2:7283750-7283769 Msa0753640:CDS 35.0%
AACAATATACGAGGGTGATT+GGG + chr5_2:7283759-7283778 Msa0753640:CDS 35.0%
!!! GTTTTGAGGGTGAATTCTTT+AGG + chr5_2:7283833-7283852 Msa0753640:CDS 35.0%
ACACCTTTACAGAATCCAAT+GGG + chr5_2:7283875-7283894 Msa0753640:CDS 35.0%
! GAATGATCTTTTCCATGGAA+AGG + chr5_2:7283990-7284009 Msa0753640:CDS 35.0%
GTCAGAAGGAGAAATATATG+AGG + chr5_2:7284026-7284045 Msa0753640:CDS 35.0%
GAGAAATATATGAGGGTGAT+TGG + chr5_2:7284034-7284053 Msa0753640:CDS 35.0%
! TATCCATCAGAAGCAAGTTT+TGG + chr5_2:7284093-7284112 Msa0753640:CDS 35.0%
! ATCCATCAGAAGCAAGTTTT+GGG + chr5_2:7284094-7284113 Msa0753640:CDS 35.0%
GACATCCTTACAAAATCTGA+CGG + chr5_2:7284149-7284168 Msa0753640:CDS 35.0%
ACATCCTTACAAAATCTGAC+GGG + chr5_2:7284150-7284169 Msa0753640:CDS 35.0%
TTCAAGAGTAGTTCTTTCCA+TGG + chr5_2:7284191-7284210 Msa0753640:CDS 35.0%
GAACAATATATGAAGGCGAT+TGG + chr5_2:7284240-7284259 Msa0753640:CDS 35.0%
! GTTTTTACAAGGGAAACCGT+AGG + chr5_2:7283704-7283723 Msa0753640:CDS 40.0%
GGAAAGTTCACATGGTCAAA+TGG + chr5_2:7283736-7283755 Msa0753640:CDS 40.0%
GAACAATATACGAGGGTGAT+TGG + chr5_2:7283758-7283777 Msa0753640:CDS 40.0%
GACACCTTTACAGAATCCAA+TGG + chr5_2:7283874-7283893 Msa0753640:CDS 40.0%
CACCTTTACAGAATCCAATG+GGG + chr5_2:7283876-7283895 Msa0753640:CDS 40.0%
ATCCCCATTGGATTCTGTAA+AGG - chr5_2:7283881-7283900 None:intergenic 40.0%
ATGTGTACTTTCCCTTTCCA+TGG - chr5_2:7284005-7284024 Msa0753650:CDS 40.0%
! ATGGAAAGGGAAAGTACACA+TGG + chr5_2:7284004-7284023 Msa0753640:CDS 40.0%
! TCCATCAGAAGCAAGTTTTG+GGG + chr5_2:7284095-7284114 Msa0753640:CDS 40.0%
CATCCTTACAAAATCTGACG+GGG + chr5_2:7284151-7284170 Msa0753640:CDS 40.0%
! TATCCCCGTCAGATTTTGTA+AGG - chr5_2:7284157-7284176 Msa0753650:CDS 40.0%
AGTAGTTCTTTCCATGGCAA+GGG + chr5_2:7284197-7284216 Msa0753640:CDS 40.0%
! GGAAAGTACACATGGTCAAA+TGG + chr5_2:7284218-7284237 Msa0753640:CDS 40.0%
GGACGAATCATATATCCATC+AGG + chr5_2:7284081-7284100 Msa0753640:CDS 40.0%
! TCCATCAGGAACAAGTTTTG+AGG + chr5_2:7284301-7284320 Msa0753640:CDS 40.0%
!! CCATCAGGAACAAGTTTTGA+GGG + chr5_2:7284302-7284321 Msa0753640:CDS 40.0%
CCAAGTGGGAATGTCTACAT+CGG + chr5_2:7283682-7283701 Msa0753640:CDS 45.0%
ATGGCAAGGGAAAGTTCACA+TGG + chr5_2:7283728-7283747 Msa0753640:CDS 45.0%
!! GTGATTGGGTTGATGGAGAA+AGG + chr5_2:7283773-7283792 Msa0753640:CDS 45.0%
ATGGAGAAAGGACAGGGAAA+GGG + chr5_2:7283785-7283804 Msa0753640:CDS 45.0%
GGGCAGATCATATATCCATC+AGG + chr5_2:7283805-7283824 Msa0753640:CDS 45.0%
! TCCATCAGGAGCAAGTTTTG+AGG + chr5_2:7283819-7283838 Msa0753640:CDS 45.0%
!! CCATCAGGAGCAAGTTTTGA+GGG + chr5_2:7283820-7283839 Msa0753640:CDS 45.0%
! TTCTTTAGGGATTCCCGTCA+TGG + chr5_2:7283847-7283866 Msa0753640:CDS 45.0%
!! TAAAGGTGTCGTGACCATGA+CGG - chr5_2:7283864-7283883 None:intergenic 45.0%
TCCAATGGGGATGTCTACAT+TGG + chr5_2:7283889-7283908 Msa0753640:CDS 45.0%
ACCAATGTAGACATCCCCAT+TGG - chr5_2:7283893-7283912 None:intergenic 45.0%
TCCAATGGGGATGTCTACAT+TGG + chr5_2:7283889-7283908 Msa0753640:CDS 45.0%
ACCAATGTAGACATCCCCAT+TGG - chr5_2:7283893-7283912 None:intergenic 45.0%
! GGAAAGTACACATGGTCAGA+AGG + chr5_2:7284012-7284031 Msa0753640:CDS 45.0%
!! TATGAGGGTGATTGGGTTGA+TGG + chr5_2:7284042-7284061 Msa0753640:CDS 45.0%
! ACCCCAAAACTTGCTTCTGA+TGG - chr5_2:7284099-7284118 Msa0753650:CDS 45.0%
!!! AAGTTTTGGGGTGAACTCTC+TGG + chr5_2:7284107-7284126 Msa0753640:CDS 45.0%
!!! AGTTTTGGGGTGAACTCTCT+GGG + chr5_2:7284108-7284127 Msa0753640:CDS 45.0%
TAAGGATGTCGTGACCATGA+CGG - chr5_2:7284139-7284158 Msa0753650:CDS 45.0%
TCTGACGGGGATATCTACAT+TGG + chr5_2:7284164-7284183 Msa0753640:CDS 45.0%
GAGTAGTTCTTTCCATGGCA+AGG + chr5_2:7284196-7284215 Msa0753640:CDS 45.0%
ATGTGTACTTTCCCTTGCCA+TGG - chr5_2:7284211-7284230 None:intergenic 45.0%
! ATGGCAAGGGAAAGTACACA+TGG + chr5_2:7284210-7284229 Msa0753640:CDS 45.0%
!! TATGAAGGCGATTGGGTTGA+TGG + chr5_2:7284248-7284267 Msa0753640:CDS 45.0%
!! TTGGGTTGATGGAAGAAGGA+CGG + chr5_2:7284259-7284278 Msa0753640:CDS 45.0%
! CCCTCAAAACTTGTTCCTGA+TGG - chr5_2:7284305-7284324 None:intergenic 45.0%
CCGATGTAGACATTCCCACT+TGG - chr5_2:7283685-7283704 None:intergenic 50.0%
TACAAGGGAAACCGTAGGCA+TGG + chr5_2:7283709-7283728 Msa0753640:CDS 50.0%
ACTTTCCCTTGCCATGCCTA+CGG - chr5_2:7283723-7283742 None:intergenic 50.0%
!! TACGAGGGTGATTGGGTTGA+TGG + chr5_2:7283766-7283785 Msa0753640:CDS 50.0%
!! TGGGTTGATGGAGAAAGGAC+AGG + chr5_2:7283778-7283797 Msa0753640:CDS 50.0%
!! GGGTTGATGGAGAAAGGACA+GGG + chr5_2:7283779-7283798 Msa0753640:CDS 50.0%
GATGGAGAAAGGACAGGGAA+AGG + chr5_2:7283784-7283803 Msa0753640:CDS 50.0%
! CCCTCAAAACTTGCTCCTGA+TGG - chr5_2:7283823-7283842 None:intergenic 50.0%
!! AAAGGTGTCGTGACCATGAC+GGG - chr5_2:7283863-7283882 None:intergenic 50.0%
!! GTGATTGGGTTGATGGAAGC+AGG + chr5_2:7284049-7284068 Msa0753640:CDS 50.0%
AAGGATGTCGTGACCATGAC+GGG - chr5_2:7284138-7284157 Msa0753650:CDS 50.0%
!! GCGATTGGGTTGATGGAAGA+AGG + chr5_2:7284255-7284274 Msa0753640:CDS 50.0%
!! TGGGTTGATGGAAGAAGGAC+GGG + chr5_2:7284260-7284279 Msa0753640:CDS 50.0%
GGAAACCGTAGGCATGGCAA+GGG + chr5_2:7283715-7283734 Msa0753640:CDS 55.0%
!! TGGGTTGATGGAAGCAGGAC+AGG + chr5_2:7284054-7284073 Msa0753640:CDS 55.0%
!! GGGTTGATGGAAGCAGGACA+GGG + chr5_2:7284055-7284074 Msa0753640:CDS 55.0%
GATGGAAGCAGGACAGGGAA+AGG + chr5_2:7284060-7284079 Msa0753640:CDS 55.0%
! CTCTCTGGGAATTCCCGTCA+TGG + chr5_2:7284122-7284141 Msa0753640:CDS 55.0%
!! GGGTTGATGGAAGAAGGACG+GGG + chr5_2:7284261-7284280 Msa0753640:CDS 55.0%
GATGGAAGAAGGACGGGGAA+AGG + chr5_2:7284266-7284285 Msa0753640:CDS 55.0%
GGGAAACCGTAGGCATGGCA+AGG + chr5_2:7283714-7283733 Msa0753640:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7283655 7284338 7283655 ID=Msa0753640;Name=Msa0753640
chr5_2 mRNA 7283655 7284338 7283655 ID=Msa0753640-mRNA-1;Parent=Msa0753640;Name=Msa0753640-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|227
chr5_2 exon 7283655 7284338 7283655 ID=Msa0753640-mRNA-1:exon:3271;Parent=Msa0753640-mRNA-1
chr5_2 CDS 7283655 7284338 7283655 ID=Msa0753640-mRNA-1:cds;Parent=Msa0753640-mRNA-1
Gene Sequence

>Msa0753640

ATGCCTAGGTTTAAAGTGAAGTATTTTCCAAGTGGGAATGTCTACATCGGTTTTTACAAGGGAAACCGTAGGCATGGCAAGGGAAAGTTCACATGGTCAAATGGAACAATATACGAGGGTGATTGGGTTGATGGAGAAAGGACAGGGAAAGGGCAGATCATATATCCATCAGGAGCAAGTTTTGAGGGTGAATTCTTTAGGGATTCCCGTCATGGTCACGACACCTTTACAGAATCCAATGGGGATGTCTACATTGGTAACTACGAGAATGATCTTTGTTATGGTCACGACACCTTTATAAAATCCAATGGGGATGTCTACATTGGTAACTTTGAGAATGATCTTTTCCATGGAAAGGGAAAGTACACATGGTCAGAAGGAGAAATATATGAGGGTGATTGGGTTGATGGAAGCAGGACAGGGAAAGGACGAATCATATATCCATCAGAAGCAAGTTTTGGGGTGAACTCTCTGGGAATTCCCGTCATGGTCACGACATCCTTACAAAATCTGACGGGGATATCTACATTGGTAACTTCAAGAGTAGTTCTTTCCATGGCAAGGGAAAGTACACATGGTCAAATGGAACAATATATGAAGGCGATTGGGTTGATGGAAGAAGGACGGGGAAAGGACGAATCATATATCCATCAGGAACAAGTTTTGAGGGTGAATTCTCTATGA
Protein sequence

>Msa0753640

MPRFKVKYFPSGNVYIGFYKGNRRHGKGKFTWSNGTIYEGDWVDGERTGKGQIIYPSGASFEGEFFRDSRHGHDTFTESNGDVYIGNYENDLCYGHDTFIKSNGDVYIGNFENDLFHGKGKYTWSEGEIYEGDWVDGSRTGKGRIIYPSEASFGVNSLGIPVMVTTSLQNLTGISTLVTSRVVLSMARESTHGQMEQYMKAIGLMEEGRGKDESYIHQEQVLRVNSL*