Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754550 | XP_003618127.1 | 83.007 | 153 | 14 | 5 | 1 | 143 | 1 | 151 | 2.40e-69 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754550 | G7KFG9 | 83.007 | 153 | 14 | 5 | 1 | 143 | 1 | 151 | 1.15e-69 | 216 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754550 | Msa1367670 | 0.976201 | 2.245083e-141 | -8.615850e-47 |
| Msa0754550 | Msa1407180 | 0.889063 | 3.387755e-73 | -8.615850e-47 |
| Msa0754550 | Msa1432120 | 0.982136 | 2.533943e-154 | -8.615850e-47 |
| Msa0216740 | Msa0754550 | 0.824667 | 7.130486e-54 | -8.615850e-47 |
| Msa0295960 | Msa0754550 | 0.835232 | 1.891273e-56 | -8.615850e-47 |
| Msa0333950 | Msa0754550 | 0.834719 | 2.547320e-56 | -8.615850e-47 |
| Msa0334060 | Msa0754550 | 0.834606 | 2.720115e-56 | -8.615850e-47 |
| Msa0514460 | Msa0754550 | 0.810869 | 9.302575e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754550 | MtrunA17_Chr5g0448731 | 83.007 | 153 | 14 | 5 | 1 | 143 | 1 | 151 | 2.21e-73 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754550 | AT4G35320.1 | 44.444 | 126 | 65 | 2 | 13 | 134 | 15 | 139 | 1.89e-21 | 84.3 |
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAGCAGCATCTCCTAATT+TGG | 0.208008 | 5_2:-8119142 | None:intergenic |
| AGCATCTCCTAATTTGGTTT+TGG | 0.264987 | 5_2:-8119136 | None:intergenic |
| GTGTCAACCAAAACCAAATT+AGG | 0.315228 | 5_2:+8119129 | Msa0754550:CDS |
| TCAAGATCTGTTGCAAAGAT+AGG | 0.315447 | 5_2:-8119336 | None:intergenic |
| CCCTTTCCACTTCTACTTGT+TGG | 0.332543 | 5_2:-8119096 | None:intergenic |
| GTTGGAAACATGTTATTTGC+AGG | 0.354673 | 5_2:+8119406 | Msa0754550:CDS |
| GGTGGACCTGGAGCTGGGCC+TGG | 0.359670 | 5_2:+8119298 | Msa0754550:CDS |
| TGAGAATGACGACTTGGATC+TGG | 0.361698 | 5_2:-8119244 | None:intergenic |
| TGGGCGACTCTATCAGAAAT+TGG | 0.371725 | 5_2:+8119277 | Msa0754550:CDS |
| TTGCTGACTTCCCGTGATAA+GGG | 0.402825 | 5_2:+8119381 | Msa0754550:CDS |
| GTCATTCTCATTCTCATCCA+TGG | 0.415773 | 5_2:+8119257 | Msa0754550:CDS |
| GTTGCTGACTTCCCGTGATA+AGG | 0.430057 | 5_2:+8119380 | Msa0754550:CDS |
| GATGCTGCTGCTGCTGATGC+AGG | 0.432107 | 5_2:+8119153 | Msa0754550:CDS |
| GAAATTGGTGGACCTGGAGC+TGG | 0.438969 | 5_2:+8119292 | Msa0754550:CDS |
| GCCGACATGGTTTGAGGAAG+AGG | 0.443482 | 5_2:-8119480 | None:intergenic |
| TGCGTAGTAATAATCAGAAT+TGG | 0.448686 | 5_2:-8119457 | None:intergenic |
| CAGTGAGAGAGGAAGTTGAT+AGG | 0.455790 | 5_2:-8119194 | None:intergenic |
| GCTGCTGCTGCTGATGCAGG+AGG | 0.460455 | 5_2:+8119156 | Msa0754550:CDS |
| GAGGATCCAGGCCCAGCTCC+AGG | 0.463280 | 5_2:-8119304 | None:intergenic |
| CTATCAGAAATTGGTGGACC+TGG | 0.485539 | 5_2:+8119286 | Msa0754550:CDS |
| AGATCTTGAAATGAATGAGC+AGG | 0.491670 | 5_2:+8119350 | Msa0754550:CDS |
| CCAACAAGTAGAAGTGGAAA+GGG | 0.494860 | 5_2:+8119096 | Msa0754550:CDS |
| CAAAGATAGGCTTCCTTGAG+AGG | 0.500750 | 5_2:-8119323 | None:intergenic |
| ATCACGGGAAGTCAGCAACT+TGG | 0.507000 | 5_2:-8119376 | None:intergenic |
| GAGAATGACGACTTGGATCT+GGG | 0.517935 | 5_2:-8119243 | None:intergenic |
| AACATGTTATTTGCAGGATG+AGG | 0.535337 | 5_2:+8119412 | Msa0754550:CDS |
| GTTTCCAACTCCCCTTATCA+CGG | 0.535374 | 5_2:-8119392 | None:intergenic |
| TCATTCTCATTCTCATCCAT+GGG | 0.545509 | 5_2:+8119258 | Msa0754550:CDS |
| AAATTGGTGGACCTGGAGCT+GGG | 0.557795 | 5_2:+8119293 | Msa0754550:CDS |
| GTGTAAGCCGACATGGTTTG+AGG | 0.558720 | 5_2:-8119486 | None:intergenic |
| AGGCTTCCTTGAGAGGATCC+AGG | 0.559333 | 5_2:-8119316 | None:intergenic |
| ACCAACAAGTAGAAGTGGAA+AGG | 0.562172 | 5_2:+8119095 | Msa0754550:CDS |
| TTTCCAACTCCCCTTATCAC+GGG | 0.565961 | 5_2:-8119391 | None:intergenic |
| TTCTTACCAACAAGTAGAAG+TGG | 0.570154 | 5_2:+8119090 | Msa0754550:CDS |
| TGTTGTTTCTGCAGTGAGAG+AGG | 0.590813 | 5_2:-8119205 | None:intergenic |
| TGCTGACTTCCCGTGATAAG+GGG | 0.596069 | 5_2:+8119382 | Msa0754550:CDS |
| TCATTGTGTGTAAGCCGACA+TGG | 0.600825 | 5_2:-8119493 | None:intergenic |
| GCGACTCTATCAGAAATTGG+TGG | 0.617542 | 5_2:+8119280 | Msa0754550:CDS |
| TGAGAATGAGAATGACGACT+TGG | 0.638362 | 5_2:-8119250 | None:intergenic |
| ATTTCTGATAGAGTCGCCCA+TGG | 0.647698 | 5_2:-8119274 | None:intergenic |
| ACCTCTTCCTCAAACCATGT+CGG | 0.673431 | 5_2:+8119479 | Msa0754550:CDS |
| CAACAAGTAGAAGTGGAAAG+GGG | 0.687682 | 5_2:+8119097 | Msa0754550:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGCGTAGTAATAATCAGAAT+TGG | - | chr5_2:8119460-8119479 | None:intergenic | 30.0% | |
| TTCTTACCAACAAGTAGAAG+TGG | + | chr5_2:8119090-8119109 | Msa0754550:CDS | 35.0% | |
| !!! | ATTTGGTTTTGGTTGACACA+GGG | - | chr5_2:8119128-8119147 | None:intergenic | 35.0% |
| !!! | AATTTGGTTTTGGTTGACAC+AGG | - | chr5_2:8119129-8119148 | None:intergenic | 35.0% |
| GTGTCAACCAAAACCAAATT+AGG | + | chr5_2:8119129-8119148 | Msa0754550:CDS | 35.0% | |
| !! | AGCATCTCCTAATTTGGTTT+TGG | - | chr5_2:8119139-8119158 | None:intergenic | 35.0% |
| TCATTCTCATTCTCATCCAT+GGG | + | chr5_2:8119258-8119277 | Msa0754550:CDS | 35.0% | |
| TCAAGATCTGTTGCAAAGAT+AGG | - | chr5_2:8119339-8119358 | None:intergenic | 35.0% | |
| AGATCTTGAAATGAATGAGC+AGG | + | chr5_2:8119350-8119369 | Msa0754550:CDS | 35.0% | |
| ! | GTTGGAAACATGTTATTTGC+AGG | + | chr5_2:8119406-8119425 | Msa0754550:CDS | 35.0% |
| ! | AACATGTTATTTGCAGGATG+AGG | + | chr5_2:8119412-8119431 | Msa0754550:CDS | 35.0% |
| ACCAACAAGTAGAAGTGGAA+AGG | + | chr5_2:8119095-8119114 | Msa0754550:CDS | 40.0% | |
| CCAACAAGTAGAAGTGGAAA+GGG | + | chr5_2:8119096-8119115 | Msa0754550:CDS | 40.0% | |
| CAACAAGTAGAAGTGGAAAG+GGG | + | chr5_2:8119097-8119116 | Msa0754550:CDS | 40.0% | |
| TGAGAATGAGAATGACGACT+TGG | - | chr5_2:8119253-8119272 | None:intergenic | 40.0% | |
| GTCATTCTCATTCTCATCCA+TGG | + | chr5_2:8119257-8119276 | Msa0754550:CDS | 40.0% | |
| CCCTTTCCACTTCTACTTGT+TGG | - | chr5_2:8119099-8119118 | None:intergenic | 45.0% | |
| AGCAGCAGCATCTCCTAATT+TGG | - | chr5_2:8119145-8119164 | None:intergenic | 45.0% | |
| ! | CAGTGAGAGAGGAAGTTGAT+AGG | - | chr5_2:8119197-8119216 | None:intergenic | 45.0% |
| !! | TGTTGTTTCTGCAGTGAGAG+AGG | - | chr5_2:8119208-8119227 | None:intergenic | 45.0% |
| ! | GAGAATGACGACTTGGATCT+GGG | - | chr5_2:8119246-8119265 | None:intergenic | 45.0% |
| ! | TGAGAATGACGACTTGGATC+TGG | - | chr5_2:8119247-8119266 | None:intergenic | 45.0% |
| ATTTCTGATAGAGTCGCCCA+TGG | - | chr5_2:8119277-8119296 | None:intergenic | 45.0% | |
| TGGGCGACTCTATCAGAAAT+TGG | + | chr5_2:8119277-8119296 | Msa0754550:CDS | 45.0% | |
| GCGACTCTATCAGAAATTGG+TGG | + | chr5_2:8119280-8119299 | Msa0754550:CDS | 45.0% | |
| ! | CTATCAGAAATTGGTGGACC+TGG | + | chr5_2:8119286-8119305 | Msa0754550:CDS | 45.0% |
| CAAAGATAGGCTTCCTTGAG+AGG | - | chr5_2:8119326-8119345 | None:intergenic | 45.0% | |
| !! | TTGCTGACTTCCCGTGATAA+GGG | + | chr5_2:8119381-8119400 | Msa0754550:CDS | 45.0% |
| TTTCCAACTCCCCTTATCAC+GGG | - | chr5_2:8119394-8119413 | None:intergenic | 45.0% | |
| GTTTCCAACTCCCCTTATCA+CGG | - | chr5_2:8119395-8119414 | None:intergenic | 45.0% | |
| ACCTCTTCCTCAAACCATGT+CGG | + | chr5_2:8119479-8119498 | Msa0754550:CDS | 45.0% | |
| TCATTGTGTGTAAGCCGACA+TGG | - | chr5_2:8119496-8119515 | None:intergenic | 45.0% | |
| ! | AAATTGGTGGACCTGGAGCT+GGG | + | chr5_2:8119293-8119312 | Msa0754550:CDS | 50.0% |
| ATCACGGGAAGTCAGCAACT+TGG | - | chr5_2:8119379-8119398 | None:intergenic | 50.0% | |
| !! | GTTGCTGACTTCCCGTGATA+AGG | + | chr5_2:8119380-8119399 | Msa0754550:CDS | 50.0% |
| !! | TGCTGACTTCCCGTGATAAG+GGG | + | chr5_2:8119382-8119401 | Msa0754550:CDS | 50.0% |
| GTGTAAGCCGACATGGTTTG+AGG | - | chr5_2:8119489-8119508 | None:intergenic | 50.0% | |
| ! | GAAATTGGTGGACCTGGAGC+TGG | + | chr5_2:8119292-8119311 | Msa0754550:CDS | 55.0% |
| AGGCTTCCTTGAGAGGATCC+AGG | - | chr5_2:8119319-8119338 | None:intergenic | 55.0% | |
| CTTCCCGTGATAAGGGGAGT+TGG | + | chr5_2:8119388-8119407 | Msa0754550:CDS | 55.0% | |
| GCCGACATGGTTTGAGGAAG+AGG | - | chr5_2:8119483-8119502 | None:intergenic | 55.0% | |
| GATGCTGCTGCTGCTGATGC+AGG | + | chr5_2:8119153-8119172 | Msa0754550:CDS | 60.0% | |
| GCTGCTGCTGCTGATGCAGG+AGG | + | chr5_2:8119156-8119175 | Msa0754550:CDS | 65.0% | |
| GCTGGGCCTGGATCCTCTCA+AGG | + | chr5_2:8119310-8119329 | Msa0754550:CDS | 65.0% | |
| GAGGATCCAGGCCCAGCTCC+AGG | - | chr5_2:8119307-8119326 | None:intergenic | 70.0% | |
| GGTGGACCTGGAGCTGGGCC+TGG | + | chr5_2:8119298-8119317 | Msa0754550:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 8119084 | 8119515 | 8119084 | ID=Msa0754550;Name=Msa0754550 |
| chr5_2 | mRNA | 8119084 | 8119515 | 8119084 | ID=Msa0754550-mRNA-1;Parent=Msa0754550;Name=Msa0754550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|143 |
| chr5_2 | exon | 8119084 | 8119515 | 8119084 | ID=Msa0754550-mRNA-1:exon:3724;Parent=Msa0754550-mRNA-1 |
| chr5_2 | CDS | 8119084 | 8119515 | 8119084 | ID=Msa0754550-mRNA-1:cds;Parent=Msa0754550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
