Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754780 | G7JXH1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.02e-32 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754780 | Msa0834170 | 0.842757 | 2.127984e-58 | -8.615850e-47 |
| Msa0754780 | Msa1388080 | 0.922103 | 1.519258e-88 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754780 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.16e-35 | 114 |
| Msa0754780 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 9 | 53 | 37 | 81 | 2.87e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754780 | AT2G35658.3 | 53.571 | 56 | 25 | 1 | 2 | 56 | 33 | 88 | 7.06e-18 | 70.5 |
| Msa0754780 | AT2G35658.1 | 52.830 | 53 | 24 | 1 | 2 | 53 | 33 | 85 | 1.79e-16 | 66.6 |
| Msa0754780 | AT2G35658.2 | 50.909 | 55 | 26 | 1 | 2 | 55 | 33 | 87 | 1.82e-16 | 67.0 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | 5_2:-8485372 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | 5_2:+8485622 | Msa0754780:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | 5_2:+8485214 | Msa0754780:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | 5_2:+8485407 | Msa0754780:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | 5_2:+8485429 | Msa0754780:CDS |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | 5_2:+8485735 | Msa0754780:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | 5_2:-8485371 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | 5_2:-8485441 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | 5_2:+8485713 | Msa0754780:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | 5_2:+8485327 | Msa0754780:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | 5_2:+8485562 | Msa0754780:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | 5_2:-8485206 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | 5_2:+8485204 | Msa0754780:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | 5_2:+8485701 | Msa0754780:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | 5_2:-8485442 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | 5_2:-8485306 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | 5_2:-8485708 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | 5_2:+8485539 | Msa0754780:intron |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | 5_2:-8485394 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | 5_2:-8485325 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | 5_2:-8485332 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | 5_2:-8485313 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | 5_2:+8485209 | Msa0754780:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | 5_2:-8485333 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | 5_2:-8485393 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | 5_2:-8485423 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | 5_2:-8485440 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | 5_2:+8485267 | Msa0754780:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | + | chr5_2:8485622-8485641 | Msa0754780:three_prime_UTR | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | - | chr5_2:8485792-8485811 | None:intergenic | 15.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | + | chr5_2:8485466-8485485 | Msa0754780:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | - | chr5_2:8485502-8485521 | None:intergenic | 20.0% |
| !! | ACTATTTCGTGTTTTTCTTT+TGG | - | chr5_2:8485167-8485186 | None:intergenic | 25.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | + | chr5_2:8485267-8485286 | Msa0754780:exon | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | + | chr5_2:8485539-8485558 | Msa0754780:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | + | chr5_2:8485735-8485754 | Msa0754780:three_prime_UTR | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | + | chr5_2:8485701-8485720 | Msa0754780:three_prime_UTR | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | + | chr5_2:8485713-8485732 | Msa0754780:three_prime_UTR | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | + | chr5_2:8485214-8485233 | Msa0754780:five_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | - | chr5_2:8485374-8485393 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | - | chr5_2:8485396-8485415 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | - | chr5_2:8485444-8485463 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | - | chr5_2:8485445-8485464 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | + | chr5_2:8485562-8485581 | Msa0754780:three_prime_UTR | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | - | chr5_2:8485711-8485730 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | + | chr5_2:8485204-8485223 | Msa0754780:five_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | - | chr5_2:8485209-8485228 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | + | chr5_2:8485209-8485228 | Msa0754780:five_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | - | chr5_2:8485335-8485354 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | - | chr5_2:8485336-8485355 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | - | chr5_2:8485375-8485394 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | - | chr5_2:8485397-8485416 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | + | chr5_2:8485407-8485426 | Msa0754780:CDS | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | - | chr5_2:8485443-8485462 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | - | chr5_2:8485328-8485347 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | + | chr5_2:8485327-8485346 | Msa0754780:CDS | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | + | chr5_2:8485429-8485448 | Msa0754780:CDS | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | - | chr5_2:8485309-8485328 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | - | chr5_2:8485426-8485445 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | - | chr5_2:8485316-8485335 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 8485157 | 8485852 | 8485157 | ID=Msa0754780;Name=Msa0754780 |
| chr5_2 | mRNA | 8485157 | 8485852 | 8485157 | ID=Msa0754780-mRNA-1;Parent=Msa0754780;Name=Msa0754780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=129|1|1|1|1|1|2|302|57 |
| chr5_2 | exon | 8485157 | 8485450 | 8485157 | ID=Msa0754780-mRNA-1:exon:3857;Parent=Msa0754780-mRNA-1 |
| chr5_2 | exon | 8485542 | 8485852 | 8485542 | ID=Msa0754780-mRNA-1:exon:3858;Parent=Msa0754780-mRNA-1 |
| chr5_2 | five_prime_UTR | 8485157 | 8485285 | 8485157 | ID=Msa0754780-mRNA-1:five_prime_utr;Parent=Msa0754780-mRNA-1 |
| chr5_2 | CDS | 8485286 | 8485450 | 8485286 | ID=Msa0754780-mRNA-1:cds;Parent=Msa0754780-mRNA-1 |
| chr5_2 | CDS | 8485542 | 8485550 | 8485542 | ID=Msa0754780-mRNA-1:cds;Parent=Msa0754780-mRNA-1 |
| chr5_2 | three_prime_UTR | 8485551 | 8485852 | 8485551 | ID=Msa0754780-mRNA-1:three_prime_utr;Parent=Msa0754780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
