Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388080 | AES94328.1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.26e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388080 | G7JXH1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.02e-32 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754780 | Msa1388080 | 0.922103 | 1.519258e-88 | -8.615850e-47 |
| Msa0796620 | Msa1388080 | 0.828976 | 6.663753e-55 | -8.615850e-47 |
| Msa0833900 | Msa1388080 | 0.854946 | 8.771578e-62 | -8.615850e-47 |
| Msa0834170 | Msa1388080 | 0.918620 | 1.244217e-86 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388080 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.16e-35 | 114 |
| Msa1388080 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 9 | 53 | 37 | 81 | 2.87e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388080 | AT2G35658.3 | 53.571 | 56 | 25 | 1 | 2 | 56 | 33 | 88 | 7.06e-18 | 70.5 |
| Msa1388080 | AT2G35658.1 | 52.830 | 53 | 24 | 1 | 2 | 53 | 33 | 85 | 1.79e-16 | 66.6 |
| Msa1388080 | AT2G35658.2 | 50.909 | 55 | 26 | 1 | 2 | 55 | 33 | 87 | 1.82e-16 | 67.0 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | tig0021032:+96008 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | tig0021032:-95758 | Msa1388080:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | tig0021032:-96166 | Msa1388080:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | tig0021032:-95973 | Msa1388080:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | tig0021032:-95951 | Msa1388080:intron |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | tig0021032:-95645 | Msa1388080:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | tig0021032:+96009 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | tig0021032:+95939 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | tig0021032:-95667 | Msa1388080:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | tig0021032:-96053 | Msa1388080:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | tig0021032:-95818 | Msa1388080:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | tig0021032:+96174 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | tig0021032:-96176 | Msa1388080:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | tig0021032:-95679 | Msa1388080:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | tig0021032:+95938 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | tig0021032:+96074 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | tig0021032:+95672 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | tig0021032:-95841 | Msa1388080:exon |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | tig0021032:+95986 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | tig0021032:+96055 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | tig0021032:+96048 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | tig0021032:+96067 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | tig0021032:-96171 | Msa1388080:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | tig0021032:+96047 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | tig0021032:+95987 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | tig0021032:+95957 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | tig0021032:+95940 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | tig0021032:-96113 | Msa1388080:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | - | tig0021032:96014-96033 | Msa1388080:CDS | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | + | tig0021032:96184-96203 | None:intergenic | 15.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | - | tig0021032:95858-95877 | Msa1388080:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | + | tig0021032:95894-95913 | None:intergenic | 20.0% |
| !! | ACTATTTCGTGTTTTTCTTT+TGG | + | tig0021032:95560-95579 | None:intergenic | 25.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | - | tig0021032:95659-95678 | Msa1388080:three_prime_UTR | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | - | tig0021032:95931-95950 | Msa1388080:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | - | tig0021032:96127-96146 | Msa1388080:five_prime_UTR | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | - | tig0021032:96093-96112 | Msa1388080:CDS | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | - | tig0021032:96105-96124 | Msa1388080:exon | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | - | tig0021032:95606-95625 | Msa1388080:three_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | + | tig0021032:95766-95785 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | + | tig0021032:95788-95807 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | + | tig0021032:95836-95855 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | + | tig0021032:95837-95856 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | - | tig0021032:95954-95973 | Msa1388080:CDS | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | + | tig0021032:96103-96122 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | - | tig0021032:95596-95615 | Msa1388080:three_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | + | tig0021032:95601-95620 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | - | tig0021032:95601-95620 | Msa1388080:three_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | + | tig0021032:95727-95746 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | + | tig0021032:95728-95747 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | + | tig0021032:95767-95786 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | + | tig0021032:95789-95808 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | - | tig0021032:95799-95818 | Msa1388080:three_prime_UTR | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | + | tig0021032:95835-95854 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | + | tig0021032:95720-95739 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | - | tig0021032:95719-95738 | Msa1388080:three_prime_UTR | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | - | tig0021032:95821-95840 | Msa1388080:three_prime_UTR | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | + | tig0021032:95701-95720 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | + | tig0021032:95818-95837 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | + | tig0021032:95708-95727 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021032 | gene | 95550 | 96244 | 95550 | ID=Msa1388080;Name=Msa1388080 |
| tig0021032 | mRNA | 95550 | 96244 | 95550 | ID=Msa1388080-mRNA-1;Parent=Msa1388080;Name=Msa1388080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=128|1|1|1|1|1|2|302|57 |
| tig0021032 | exon | 95550 | 95860 | 95550 | ID=Msa1388080-mRNA-1:exon:10944;Parent=Msa1388080-mRNA-1 |
| tig0021032 | exon | 95952 | 96244 | 95952 | ID=Msa1388080-mRNA-1:exon:10943;Parent=Msa1388080-mRNA-1 |
| tig0021032 | five_prime_UTR | 96117 | 96244 | 96117 | ID=Msa1388080-mRNA-1:five_prime_utr;Parent=Msa1388080-mRNA-1 |
| tig0021032 | CDS | 95952 | 96116 | 95952 | ID=Msa1388080-mRNA-1:cds;Parent=Msa1388080-mRNA-1 |
| tig0021032 | CDS | 95852 | 95860 | 95852 | ID=Msa1388080-mRNA-1:cds;Parent=Msa1388080-mRNA-1 |
| tig0021032 | three_prime_UTR | 95550 | 95851 | 95550 | ID=Msa1388080-mRNA-1:three_prime_utr;Parent=Msa1388080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
