Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0834170 | AES94328.1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.26e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0834170 | G7JXH1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.02e-32 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754780 | Msa0834170 | 0.842757 | 2.127984e-58 | -8.615850e-47 |
| Msa0754820 | Msa0834170 | 0.845078 | 5.081919e-59 | -8.615850e-47 |
| Msa0796620 | Msa0834170 | 0.952847 | 9.989519e-111 | -8.615850e-47 |
| Msa0833900 | Msa0834170 | 0.957987 | 7.145791e-116 | -8.615850e-47 |
| Msa0834170 | Msa1388080 | 0.918620 | 1.244217e-86 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0834170 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.16e-35 | 114 |
| Msa0834170 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 9 | 53 | 37 | 81 | 2.87e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0834170 | AT2G35658.3 | 53.571 | 56 | 25 | 1 | 2 | 56 | 33 | 88 | 7.06e-18 | 70.5 |
| Msa0834170 | AT2G35658.1 | 52.830 | 53 | 24 | 1 | 2 | 53 | 33 | 85 | 1.79e-16 | 66.6 |
| Msa0834170 | AT2G35658.2 | 50.909 | 55 | 26 | 1 | 2 | 55 | 33 | 87 | 1.82e-16 | 67.0 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | 5_4:+7190387 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | 5_4:-7190137 | Msa0834170:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | 5_4:-7190545 | Msa0834170:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | 5_4:-7190352 | Msa0834170:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | 5_4:-7190330 | Msa0834170:intron |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | 5_4:-7190024 | Msa0834170:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | 5_4:+7190388 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | 5_4:+7190318 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | 5_4:-7190046 | Msa0834170:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | 5_4:-7190432 | Msa0834170:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | 5_4:-7190197 | Msa0834170:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | 5_4:+7190553 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | 5_4:-7190555 | Msa0834170:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | 5_4:-7190058 | Msa0834170:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | 5_4:+7190317 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | 5_4:+7190453 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | 5_4:+7190051 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | 5_4:-7190220 | Msa0834170:exon |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | 5_4:+7190365 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | 5_4:+7190434 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | 5_4:+7190427 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | 5_4:+7190446 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | 5_4:-7190550 | Msa0834170:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | 5_4:+7190426 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | 5_4:+7190366 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | 5_4:+7190336 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | 5_4:+7190319 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | 5_4:-7190492 | Msa0834170:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | - | chr5_4:7190393-7190412 | Msa0834170:CDS | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | + | chr5_4:7190563-7190582 | None:intergenic | 15.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | - | chr5_4:7190237-7190256 | Msa0834170:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | + | chr5_4:7190273-7190292 | None:intergenic | 20.0% |
| !! | ACTATTTCGTGTTTTTCTTT+TGG | + | chr5_4:7189939-7189958 | None:intergenic | 25.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | - | chr5_4:7190038-7190057 | Msa0834170:three_prime_UTR | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | - | chr5_4:7190310-7190329 | Msa0834170:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | - | chr5_4:7190506-7190525 | Msa0834170:five_prime_UTR | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | - | chr5_4:7190472-7190491 | Msa0834170:CDS | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | - | chr5_4:7190484-7190503 | Msa0834170:exon | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | - | chr5_4:7189985-7190004 | Msa0834170:three_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | + | chr5_4:7190145-7190164 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | + | chr5_4:7190167-7190186 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | + | chr5_4:7190215-7190234 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | + | chr5_4:7190216-7190235 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | - | chr5_4:7190333-7190352 | Msa0834170:CDS | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | + | chr5_4:7190482-7190501 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | - | chr5_4:7189975-7189994 | Msa0834170:three_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | + | chr5_4:7189980-7189999 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | - | chr5_4:7189980-7189999 | Msa0834170:three_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | + | chr5_4:7190106-7190125 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | + | chr5_4:7190107-7190126 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | + | chr5_4:7190146-7190165 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | + | chr5_4:7190168-7190187 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | - | chr5_4:7190178-7190197 | Msa0834170:three_prime_UTR | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | + | chr5_4:7190214-7190233 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | + | chr5_4:7190099-7190118 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | - | chr5_4:7190098-7190117 | Msa0834170:three_prime_UTR | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | - | chr5_4:7190200-7190219 | Msa0834170:three_prime_UTR | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | + | chr5_4:7190080-7190099 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | + | chr5_4:7190197-7190216 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | + | chr5_4:7190087-7190106 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 7189929 | 7190623 | 7189929 | ID=Msa0834170;Name=Msa0834170 |
| chr5_4 | mRNA | 7189929 | 7190623 | 7189929 | ID=Msa0834170-mRNA-1;Parent=Msa0834170;Name=Msa0834170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=128|1|1|1|1|1|2|302|57 |
| chr5_4 | exon | 7189929 | 7190239 | 7189929 | ID=Msa0834170-mRNA-1:exon:3222;Parent=Msa0834170-mRNA-1 |
| chr5_4 | exon | 7190331 | 7190623 | 7190331 | ID=Msa0834170-mRNA-1:exon:3221;Parent=Msa0834170-mRNA-1 |
| chr5_4 | five_prime_UTR | 7190496 | 7190623 | 7190496 | ID=Msa0834170-mRNA-1:five_prime_utr;Parent=Msa0834170-mRNA-1 |
| chr5_4 | CDS | 7190331 | 7190495 | 7190331 | ID=Msa0834170-mRNA-1:cds;Parent=Msa0834170-mRNA-1 |
| chr5_4 | CDS | 7190231 | 7190239 | 7190231 | ID=Msa0834170-mRNA-1:cds;Parent=Msa0834170-mRNA-1 |
| chr5_4 | three_prime_UTR | 7189929 | 7190230 | 7189929 | ID=Msa0834170-mRNA-1:three_prime_utr;Parent=Msa0834170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
