AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0754830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTTCACGAAAAGATTA+TGG 0.173971 5_2:-8537848 Msa0754830:five_prime_UTR
GAGAAAACGAAGAAGAAATT+AGG 0.201120 5_2:-8537532 Msa0754830:five_prime_UTR
TCTGATATGGGAAAATAATA+TGG 0.202175 5_2:+8537454 None:intergenic
ATAGTCTATTAATTTATATT+TGG 0.215508 5_2:-8537429 Msa0754830:five_prime_UTR
ATTCACTCAACATGTGAAAA+AGG 0.218510 5_2:-8537696 Msa0754830:five_prime_UTR
GAAGAAGAAGATGAAGAAAA+AGG 0.231286 5_2:+8537939 None:intergenic
AATCAAGAAAAGTCTTTGTT+TGG 0.318002 5_2:+8534730 None:intergenic
ATATGGGAAAATAATGGATT+AGG 0.320937 5_2:+8537471 None:intergenic
AGAGGGAGTGGTGGAATGTT+TGG 0.333140 5_2:+8537987 None:intergenic
AAATAAGATGAGAAAGAGTT+CGG 0.348384 5_2:+8537903 None:intergenic
TTTATGATTCATATTCATAA+TGG 0.350792 5_2:-8534533 Msa0754830:three_prime_UTR
TGCCCTTGAACTTTATCTCT+TGG 0.367137 5_2:+8534756 None:intergenic
TTCTTGATTAATTCTATTGT+AGG 0.368897 5_2:-8534716 Msa0754830:CDS
TTAATGTTGGATAGGAATAA+AGG 0.376668 5_2:+8538071 None:intergenic
ATCCTTCACGAAAAGATTAT+GGG 0.377292 5_2:-8537847 Msa0754830:five_prime_UTR
AATAAAGGAGAAAATGTATC+AGG 0.382572 5_2:+8538086 None:intergenic
AAAATAATATGGGAAAATAA+TGG 0.391252 5_2:+8537465 None:intergenic
ATGACTAATCGGATGGGAAC+AGG 0.401848 5_2:-8537339 Msa0754830:CDS
AGAAAACGAAGAAGAAATTA+GGG 0.406138 5_2:-8537531 Msa0754830:five_prime_UTR
CTGATATGGGAAAATAATAT+GGG 0.407473 5_2:+8537455 None:intergenic
CAGAGTTGTTGATGAAGGTT+AGG 0.413011 5_2:-8535736 Msa0754830:CDS
AATATCTAAAGTGGATTGGC+AGG 0.421646 5_2:-8534364 Msa0754830:three_prime_UTR
ATTCAAAGGAATGGAAATGA+AGG 0.446253 5_2:-8536138 Msa0754830:CDS
TGTAGATTTATTCAAAGGAA+TGG 0.453730 5_2:-8536147 Msa0754830:intron
TGTAACGTAGGATAGAAAAG+AGG 0.454707 5_2:+8537969 None:intergenic
TTTCACTGCTGAAAGTTTGT+CGG 0.466690 5_2:-8537730 Msa0754830:five_prime_UTR
TGGAATGAGGAAATTAATGT+TGG 0.469750 5_2:+8538058 None:intergenic
ATATGTGTAAATATCTAAAG+TGG 0.470225 5_2:-8534373 Msa0754830:three_prime_UTR
ATTCATGCCGCCACACTCAT+AGG 0.484038 5_2:-8534469 Msa0754830:three_prime_UTR
CGACCAAGAGATAAAGTTCA+AGG 0.484816 5_2:-8534759 Msa0754830:CDS
GTCTTTATCTGAGATAAAGT+TGG 0.491471 5_2:-8534801 Msa0754830:CDS
TCGTGAAGGATGCATAGAAA+AGG 0.494569 5_2:+8537859 None:intergenic
TGAGGAAATTAATGTTGGAT+AGG 0.499476 5_2:+8538063 None:intergenic
AATAAGATGAGAAAGAGTTC+GGG 0.501569 5_2:+8537904 None:intergenic
TGTAAATATCTAAAGTGGAT+TGG 0.505146 5_2:-8534368 Msa0754830:three_prime_UTR
GTTGATGAAGGTTAGGAATG+AGG 0.513138 5_2:-8535729 Msa0754830:intron
GATTCATATTCATAATGGCA+TGG 0.515799 5_2:-8534528 Msa0754830:three_prime_UTR
GTGGGAATAGATAACTTGAT+AGG 0.521772 5_2:-8534497 Msa0754830:three_prime_UTR
AGGCTCAGAGTTGTTGATGA+AGG 0.526366 5_2:-8535741 Msa0754830:CDS
GAATGTGTAGATTTATTCAA+AGG 0.527116 5_2:-8536152 Msa0754830:intron
TTTGAGAATGACTAATCGGA+TGG 0.535025 5_2:-8537346 Msa0754830:exon
GTAACGTAGGATAGAAAAGA+GGG 0.535809 5_2:+8537970 None:intergenic
ATTCATATTCATAATGGCAT+GGG 0.540099 5_2:-8534527 Msa0754830:three_prime_UTR
AAAGGTTTGTGTTGTAACGT+AGG 0.548427 5_2:+8537957 None:intergenic
TGGAAATGAAAGAGAAAGTG+AGG 0.550881 5_2:+8538007 None:intergenic
TTAGGGAAGAATAATAATCA+AGG 0.552119 5_2:-8537514 Msa0754830:five_prime_UTR
TTCAAAGGAATGGAAATGAA+GGG 0.554351 5_2:-8536137 Msa0754830:CDS
TAATGGCATGGGATCTCGTG+TGG 0.565661 5_2:-8534516 Msa0754830:three_prime_UTR
GGATAGAAAAGAGGGAGTGG+TGG 0.569012 5_2:+8537978 None:intergenic
GTAGGATAGAAAAGAGGGAG+TGG 0.582693 5_2:+8537975 None:intergenic
GGGTTTATTAGATATGACTC+AGG 0.619150 5_2:-8536117 Msa0754830:intron
TGACTAATCGGATGGGAACA+GGG 0.622507 5_2:-8537338 Msa0754830:intron
TTGAGAATGACTAATCGGAT+GGG 0.630103 5_2:-8537345 Msa0754830:exon
AATGGCATGGGATCTCGTGT+GGG 0.632770 5_2:-8534515 Msa0754830:three_prime_UTR
GACCAAGAGATAAAGTTCAA+GGG 0.637923 5_2:-8534758 Msa0754830:CDS
ATATCTAAAGTGGATTGGCA+GGG 0.647370 5_2:-8534363 Msa0754830:three_prime_UTR
TCAATTCTGACCTATGAGTG+TGG 0.668878 5_2:+8534459 None:intergenic
ATTCTGACCTATGAGTGTGG+CGG 0.701306 5_2:+8534462 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTCTATTAATTTATATT+TGG - chr5_2:8535010-8535029 Msa0754830:intron 10.0%
!!! TACTAATTTTCAAAATTATA+TGG - chr5_2:8535902-8535921 Msa0754830:intron 10.0%
!! TATAAGCTAATAAATTAATT+TGG - chr5_2:8536043-8536062 Msa0754830:intron 10.0%
!!! CATATTTTTTTTAAACAAAA+GGG + chr5_2:8537523-8537542 None:intergenic 10.0%
!!! ACATATTTTTTTTAAACAAA+AGG + chr5_2:8537524-8537543 None:intergenic 10.0%
!! AAAATAATATGGGAAAATAA+TGG + chr5_2:8534977-8534996 None:intergenic 15.0%
!!! ATTTGATGATTTTTTTAGAT+TGG - chr5_2:8535318-8535337 Msa0754830:intron 15.0%
!!! AAAAATATCTCAAATTTTGA+AGG + chr5_2:8535934-8535953 None:intergenic 15.0%
!!! TTCAAAATTTGAGATATTTT+TGG - chr5_2:8535933-8535952 Msa0754830:intron 15.0%
!! TCAATTAATGAAATACAAAT+AGG + chr5_2:8536642-8536661 None:intergenic 15.0%
!!! TTTATTATTATTTTGACAAG+AGG - chr5_2:8537792-8537811 Msa0754830:five_prime_UTR 15.0%
!!! TTATTATTATTTTGACAAGA+GGG - chr5_2:8537793-8537812 Msa0754830:five_prime_UTR 15.0%
!! TTTATGATTCATATTCATAA+TGG - chr5_2:8537906-8537925 Msa0754830:five_prime_UTR 15.0%
!! AAAATGTATCAGGAAAAAAA+GGG + chr5_2:8534346-8534365 None:intergenic 20.0%
!!! AATAGACTATTTTCTGATAT+GGG + chr5_2:8535000-8535019 None:intergenic 20.0%
!!! TAATAGACTATTTTCTGATA+TGG + chr5_2:8535001-8535020 None:intergenic 20.0%
!!! TTACTTTATCTATTTTCTGT+TGG - chr5_2:8535288-8535307 Msa0754830:intron 20.0%
!! TAATCACATAACTTATACAA+AGG + chr5_2:8535554-8535573 None:intergenic 20.0%
!! TTGTATAAGTTATGTGATTA+TGG - chr5_2:8535554-8535573 Msa0754830:intron 20.0%
!! CTTAGTATCAATTTAATGTT+AGG - chr5_2:8535637-8535656 Msa0754830:intron 20.0%
!!! ACATTGATATTTTGTTCTTT+TGG - chr5_2:8535962-8535981 Msa0754830:intron 20.0%
!!! CATTGATATTTTGTTCTTTT+GGG - chr5_2:8535963-8535982 Msa0754830:intron 20.0%
!!! ATTATCCAGAATTATTTTCT+AGG - chr5_2:8536094-8536113 Msa0754830:intron 20.0%
!! ATAATCCTAGAAAATAATTC+TGG + chr5_2:8536102-8536121 None:intergenic 20.0%
!!! AAATTTTCTCCAGTAATATA+AGG - chr5_2:8536181-8536200 Msa0754830:intron 20.0%
!! AATCAATAACCTTATATTAC+TGG + chr5_2:8536193-8536212 None:intergenic 20.0%
!! TGTTTGATGTAAAAAATGAA+TGG - chr5_2:8536499-8536518 Msa0754830:intron 20.0%
!! TTGATTGTATGTAAATTTGA+AGG - chr5_2:8536818-8536837 Msa0754830:intron 20.0%
!!! GTTATATTATTAGCTCTTAA+TGG - chr5_2:8537018-8537037 Msa0754830:intron 20.0%
!! TTGAAATATTAATGGTAAAC+AGG + chr5_2:8537097-8537116 None:intergenic 20.0%
!! TGTTTACCATTAATATTTCA+AGG - chr5_2:8537096-8537115 Msa0754830:intron 20.0%
!!! ATTAATATTTCAAGGCTTTT+GGG - chr5_2:8537104-8537123 Msa0754830:intron 20.0%
!!! ACTTGGTTTCTTTTTTAATT+TGG - chr5_2:8537274-8537293 Msa0754830:intron 20.0%
!!! TTTGAGATAAAAAAATGCTT+TGG + chr5_2:8537340-8537359 None:intergenic 20.0%
!!! AAAGTTTTTTGTTTTGCTTA+AGG - chr5_2:8537453-8537472 Msa0754830:five_prime_UTR 20.0%
!!! TTTAGAATAACATAGTACAA+AGG - chr5_2:8537551-8537570 Msa0754830:intron 20.0%
!! TTCTTGATTAATTCTATTGT+AGG - chr5_2:8537723-8537742 Msa0754830:five_prime_UTR 20.0%
!! ATATGTGTAAATATCTAAAG+TGG - chr5_2:8538066-8538085 Msa0754830:five_prime_UTR 20.0%
! GAAAATGTATCAGGAAAAAA+AGG + chr5_2:8534347-8534366 None:intergenic 25.0%
! AATAAAGGAGAAAATGTATC+AGG + chr5_2:8534356-8534375 None:intergenic 25.0%
! TTAATGTTGGATAGGAATAA+AGG + chr5_2:8534371-8534390 None:intergenic 25.0%
! AAATAAGATGAGAAAGAGTT+CGG + chr5_2:8534539-8534558 None:intergenic 25.0%
!!! CATAAACTGATTTTCGAAAA+GGG + chr5_2:8534673-8534692 None:intergenic 25.0%
!! CTTTTCGAAAATCAGTTTAT+GGG - chr5_2:8534672-8534691 Msa0754830:CDS 25.0%
! AGAGATTAACAAAGATTGAT+AGG + chr5_2:8534816-8534835 None:intergenic 25.0%
! AGAAAACGAAGAAGAAATTA+GGG - chr5_2:8534908-8534927 Msa0754830:intron 25.0%
! TTAGGGAAGAATAATAATCA+AGG - chr5_2:8534925-8534944 Msa0754830:intron 25.0%
!!! ATATGGGAAAATAATGGATT+AGG + chr5_2:8534971-8534990 None:intergenic 25.0%
! CTGATATGGGAAAATAATAT+GGG + chr5_2:8534987-8535006 None:intergenic 25.0%
! TCTGATATGGGAAAATAATA+TGG + chr5_2:8534988-8535007 None:intergenic 25.0%
!! ATTCTCAAAAGTGATTTTGA+TGG + chr5_2:8535082-8535101 None:intergenic 25.0%
!!! CAACAATTCTCTTTTAATTC+AGG - chr5_2:8535186-8535205 Msa0754830:intron 25.0%
!! CTTTTGTTGAATATGAGATT+TGG - chr5_2:8535342-8535361 Msa0754830:intron 25.0%
!! ATACAAATACCCATTTTAGA+TGG + chr5_2:8535717-8535736 None:intergenic 25.0%
!!! GGTTATTGATTTTCGTATTT+TGG - chr5_2:8536202-8536221 Msa0754830:intron 25.0%
! GAATGTGTAGATTTATTCAA+AGG - chr5_2:8536287-8536306 Msa0754830:intron 25.0%
! TGTAGATTTATTCAAAGGAA+TGG - chr5_2:8536292-8536311 Msa0754830:intron 25.0%
! CATGAAAATAACATACCTTA+TGG + chr5_2:8536396-8536415 None:intergenic 25.0%
! TGTCTCAATATGTATTAAGT+AGG - chr5_2:8536593-8536612 Msa0754830:intron 25.0%
!! CGTTTTTCTCTAATATTACT+TGG - chr5_2:8536674-8536693 Msa0754830:intron 25.0%
! ATTTGATGCATAAGTCTTTA+AGG + chr5_2:8537068-8537087 None:intergenic 25.0%
! CAAAAGCCTTGAAATATTAA+TGG + chr5_2:8537105-8537124 None:intergenic 25.0%
!! CATTAATATTTCAAGGCTTT+TGG - chr5_2:8537103-8537122 Msa0754830:intron 25.0%
! TTGGGTATAATAGCATTATT+AGG - chr5_2:8537122-8537141 Msa0754830:intron 25.0%
! TAAAAAAGAAACCAAGTTAG+AGG + chr5_2:8537271-8537290 None:intergenic 25.0%
!!! AAATGCTTTGGAATTTAAAG+TGG + chr5_2:8537328-8537347 None:intergenic 25.0%
! TTAAACAAAAGGGAAAACTA+TGG + chr5_2:8537513-8537532 None:intergenic 25.0%
! TCAGATAAAGACTACAAAAT+AGG + chr5_2:8537630-8537649 None:intergenic 25.0%
! AATCAAGAAAAGTCTTTGTT+TGG + chr5_2:8537712-8537731 None:intergenic 25.0%
! ATTCATATTCATAATGGCAT+GGG - chr5_2:8537912-8537931 Msa0754830:five_prime_UTR 25.0%
!! AATACCAATTTTGCAAAAGA+AGG - chr5_2:8538004-8538023 Msa0754830:five_prime_UTR 25.0%
!! AAAACCTTCTTTTGCAAAAT+TGG + chr5_2:8538011-8538030 None:intergenic 25.0%
!! TGTAAATATCTAAAGTGGAT+TGG - chr5_2:8538071-8538090 Msa0754830:five_prime_UTR 25.0%
TGAGGAAATTAATGTTGGAT+AGG + chr5_2:8534379-8534398 None:intergenic 30.0%
TGGAATGAGGAAATTAATGT+TGG + chr5_2:8534384-8534403 None:intergenic 30.0%
GAGAGTAAAAAAATGGAATG+AGG + chr5_2:8534397-8534416 None:intergenic 30.0%
GATGAGAGAGAGTAAAAAAA+TGG + chr5_2:8534404-8534423 None:intergenic 30.0%
GAAGAAGAAGATGAAGAAAA+AGG + chr5_2:8534503-8534522 None:intergenic 30.0%
AATAAGATGAGAAAGAGTTC+GGG + chr5_2:8534538-8534557 None:intergenic 30.0%
ATCCTTCACGAAAAGATTAT+GGG - chr5_2:8534592-8534611 Msa0754830:three_prime_UTR 30.0%
!! CCATAAACTGATTTTCGAAA+AGG + chr5_2:8534674-8534693 None:intergenic 30.0%
! CCTTTTCGAAAATCAGTTTA+TGG - chr5_2:8534671-8534690 Msa0754830:CDS 30.0%
ATTCACTCAACATGTGAAAA+AGG - chr5_2:8534743-8534762 Msa0754830:CDS 30.0%
TTCACTCAACATGTGAAAAA+GGG - chr5_2:8534744-8534763 Msa0754830:CDS 30.0%
CATGAGACTAAATGTGAAAA+GGG + chr5_2:8534852-8534871 None:intergenic 30.0%
ACATGAGACTAAATGTGAAA+AGG + chr5_2:8534853-8534872 None:intergenic 30.0%
GAGAAAACGAAGAAGAAATT+AGG - chr5_2:8534907-8534926 Msa0754830:intron 30.0%
!! CACTTTTGAGAATGACTAAT+CGG - chr5_2:8535089-8535108 Msa0754830:intron 30.0%
TTGATTTAACTACATATGCG+TGG - chr5_2:8535236-8535255 Msa0754830:intron 30.0%
TGATTTAACTACATATGCGT+GGG - chr5_2:8535237-8535256 Msa0754830:intron 30.0%
TGCACAATAGTTCAATTGAA+GGG + chr5_2:8535674-8535693 None:intergenic 30.0%
TTGCACAATAGTTCAATTGA+AGG + chr5_2:8535675-8535694 None:intergenic 30.0%
AAGTCATATGCCATCTAAAA+TGG - chr5_2:8535704-8535723 Msa0754830:intron 30.0%
! AGTCATATGCCATCTAAAAT+GGG - chr5_2:8535705-8535724 Msa0754830:intron 30.0%
!! TTTTTAGGCTTCTCTACTAT+AGG - chr5_2:8535853-8535872 Msa0754830:intron 30.0%
!!! CTTTTGGGATTCTGATTTTT+TGG - chr5_2:8535978-8535997 Msa0754830:intron 30.0%
TAACAAAATCAGTGTACTTC+AGG + chr5_2:8536260-8536279 None:intergenic 30.0%
ATTCAAAGGAATGGAAATGA+AGG - chr5_2:8536301-8536320 Msa0754830:intron 30.0%
TTCAAAGGAATGGAAATGAA+GGG - chr5_2:8536302-8536321 Msa0754830:intron 30.0%
! TCTGGTAATTTTGTTCCATA+AGG - chr5_2:8536378-8536397 Msa0754830:intron 30.0%
TTTCTCTAATATTACTTGGC+AGG - chr5_2:8536678-8536697 Msa0754830:intron 30.0%
CTCTTAGAACTATACATAGA+CGG + chr5_2:8536767-8536786 None:intergenic 30.0%
! TTCTAAGAGAATTCAGAACT+AGG - chr5_2:8536778-8536797 Msa0754830:intron 30.0%
ATTATGATCGAAGATTCACA+TGG - chr5_2:8536887-8536906 Msa0754830:intron 30.0%
! TATGAGCAGTTTGAAATGTT+TGG + chr5_2:8536929-8536948 None:intergenic 30.0%
!! TAGTCTTATAACTCTCTTCA+AGG - chr5_2:8537148-8537167 Msa0754830:intron 30.0%
! ACTCATTTTCCATTTACCAT+GGG - chr5_2:8537386-8537405 Msa0754830:five_prime_UTR 30.0%
TTTCTATATCCCATGGTAAA+TGG + chr5_2:8537398-8537417 None:intergenic 30.0%
TACATACACTTAGTAGATTG+TGG - chr5_2:8537575-8537594 Msa0754830:intron 30.0%
! GTCTTTATCTGAGATAAAGT+TGG - chr5_2:8537638-8537657 Msa0754830:intron 30.0%
GATTCATATTCATAATGGCA+TGG - chr5_2:8537911-8537930 Msa0754830:five_prime_UTR 30.0%
AAGAGAAAGTGAGGAAAAAC+AGG + chr5_2:8534426-8534445 None:intergenic 35.0%
TGGAAATGAAAGAGAAAGTG+AGG + chr5_2:8534435-8534454 None:intergenic 35.0%
GTAACGTAGGATAGAAAAGA+GGG + chr5_2:8534472-8534491 None:intergenic 35.0%
TGTAACGTAGGATAGAAAAG+AGG + chr5_2:8534473-8534492 None:intergenic 35.0%
AAAGGTTTGTGTTGTAACGT+AGG + chr5_2:8534485-8534504 None:intergenic 35.0%
CATCCTTCACGAAAAGATTA+TGG - chr5_2:8534591-8534610 Msa0754830:three_prime_UTR 35.0%
! AACCCATAATCTTTTCGTGA+AGG + chr5_2:8534597-8534616 None:intergenic 35.0%
TTTCACTGCTGAAAGTTTGT+CGG - chr5_2:8534709-8534728 Msa0754830:CDS 35.0%
!! GAGTTATTTCGTTTTCGTAC+AGG - chr5_2:8534876-8534895 Msa0754830:intron 35.0%
! TGATTCTGTTTTGTGCATGT+TGG - chr5_2:8535040-8535059 Msa0754830:intron 35.0%
TTTGAGAATGACTAATCGGA+TGG - chr5_2:8535093-8535112 Msa0754830:intron 35.0%
TTGAGAATGACTAATCGGAT+GGG - chr5_2:8535094-8535113 Msa0754830:intron 35.0%
TGGGTATAATCACTACTTGA+AGG - chr5_2:8535256-8535275 Msa0754830:intron 35.0%
AGTTACAATACCAAGCAAGT+GGG - chr5_2:8535607-8535626 Msa0754830:intron 35.0%
CATCTTGAGTTTGAGCAAAT+CGG - chr5_2:8535748-8535767 Msa0754830:intron 35.0%
!!! GCCATTTTCTGCTGTTTTTT+AGG - chr5_2:8535838-8535857 Msa0754830:intron 35.0%
GCCTAAAAAACAGCAGAAAA+TGG + chr5_2:8535842-8535861 None:intergenic 35.0%
GGGTTTATTAGATATGACTC+AGG - chr5_2:8536322-8536341 Msa0754830:intron 35.0%
GAATTGTGCTTCTTCGATTA+TGG - chr5_2:8536476-8536495 Msa0754830:intron 35.0%
! TACATAGACGGACATTTTGT+AGG + chr5_2:8536755-8536774 None:intergenic 35.0%
! ACTCTCTTCAAGGTTTTCAT+TGG - chr5_2:8537158-8537177 Msa0754830:intron 35.0%
AGAAAACGTGCAAGTAAGTT+TGG - chr5_2:8537245-8537264 Msa0754830:intron 35.0%
! GACTCATTTTCCATTTACCA+TGG - chr5_2:8537385-8537404 Msa0754830:five_prime_UTR 35.0%
! CATAGCTTTTCTATATCCCA+TGG + chr5_2:8537405-8537424 None:intergenic 35.0%
ACCTATCTTACTCTTTCAGA+TGG + chr5_2:8537479-8537498 None:intergenic 35.0%
ACAAAAGGGAAAACTATGGA+TGG + chr5_2:8537509-8537528 None:intergenic 35.0%
GACCAAGAGATAAAGTTCAA+GGG - chr5_2:8537681-8537700 Msa0754830:five_prime_UTR 35.0%
GTGGGAATAGATAACTTGAT+AGG - chr5_2:8537942-8537961 Msa0754830:five_prime_UTR 35.0%
!! AATATCTAAAGTGGATTGGC+AGG - chr5_2:8538075-8538094 Msa0754830:five_prime_UTR 35.0%
!! ATATCTAAAGTGGATTGGCA+GGG - chr5_2:8538076-8538095 Msa0754830:five_prime_UTR 35.0%
TCGTGAAGGATGCATAGAAA+AGG + chr5_2:8534583-8534602 None:intergenic 40.0%
!! AGGGATCAATTTTGAGTGTG+AGG - chr5_2:8534763-8534782 Msa0754830:CDS 40.0%
! CGTTTTCGTACAGGTTCTTT+CGG - chr5_2:8534885-8534904 Msa0754830:intron 40.0%
GGTGAAGATTCTAGACAAGA+AGG - chr5_2:8535207-8535226 Msa0754830:intron 40.0%
CAGTTACAATACCAAGCAAG+TGG - chr5_2:8535606-8535625 Msa0754830:intron 40.0%
GTTACAATACCAAGCAAGTG+GGG - chr5_2:8535608-8535627 Msa0754830:intron 40.0%
TTACAATACCAAGCAAGTGG+GGG - chr5_2:8535609-8535628 Msa0754830:intron 40.0%
AGTACGTACATCTTGATCTC+TGG - chr5_2:8536360-8536379 Msa0754830:intron 40.0%
! CAGAGTTGTTGATGAAGGTT+AGG - chr5_2:8536703-8536722 Msa0754830:intron 40.0%
! GTTGATGAAGGTTAGGAATG+AGG - chr5_2:8536710-8536729 Msa0754830:intron 40.0%
! GGTTTTCATTGGACAAAGAG+AGG - chr5_2:8537169-8537188 Msa0754830:intron 40.0%
AGTAAGTTTGGCCTCTAACT+TGG - chr5_2:8537257-8537276 Msa0754830:intron 40.0%
GCCATCTGAAAGAGTAAGAT+AGG - chr5_2:8537475-8537494 Msa0754830:five_prime_UTR 40.0%
CGACCAAGAGATAAAGTTCA+AGG - chr5_2:8537680-8537699 Msa0754830:five_prime_UTR 40.0%
TGCCCTTGAACTTTATCTCT+TGG + chr5_2:8537686-8537705 None:intergenic 40.0%
TCAATTCTGACCTATGAGTG+TGG + chr5_2:8537983-8538002 None:intergenic 40.0%
GTAGGATAGAAAAGAGGGAG+TGG + chr5_2:8534467-8534486 None:intergenic 45.0%
ATGACTAATCGGATGGGAAC+AGG - chr5_2:8535100-8535119 Msa0754830:intron 45.0%
TGACTAATCGGATGGGAACA+GGG - chr5_2:8535101-8535120 Msa0754830:intron 45.0%
! GTGAGCGCTCTAAATGTTTG+AGG - chr5_2:8536567-8536586 Msa0754830:intron 45.0%
! AGGCTCAGAGTTGTTGATGA+AGG - chr5_2:8536698-8536717 Msa0754830:intron 45.0%
ATTCTGACCTATGAGTGTGG+CGG + chr5_2:8537980-8537999 None:intergenic 45.0%
! AGAGGGAGTGGTGGAATGTT+TGG + chr5_2:8534455-8534474 None:intergenic 50.0%
GGATAGAAAAGAGGGAGTGG+TGG + chr5_2:8534464-8534483 None:intergenic 50.0%
AATACCAAGCAAGTGGGGGT+TGG - chr5_2:8535613-8535632 Msa0754830:intron 50.0%
GACCTTTGCACGTCATTGAG+TGG - chr5_2:8535816-8535835 Msa0754830:intron 50.0%
TAATGGCATGGGATCTCGTG+TGG - chr5_2:8537923-8537942 Msa0754830:five_prime_UTR 50.0%
AATGGCATGGGATCTCGTGT+GGG - chr5_2:8537924-8537943 Msa0754830:five_prime_UTR 50.0%
ATTCATGCCGCCACACTCAT+AGG - chr5_2:8537970-8537989 Msa0754830:five_prime_UTR 50.0%
AAGACCAACCCCCACTTGCT+TGG + chr5_2:8535620-8535639 None:intergenic 55.0%
GGCCACTCAATGACGTGCAA+AGG + chr5_2:8535821-8535840 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 8534335 8538126 8534335 ID=Msa0754830;Name=Msa0754830
chr5_2 mRNA 8534335 8538126 8534335 ID=Msa0754830-mRNA-1;Parent=Msa0754830;Name=Msa0754830-mRNA-1;_AED=0.39;_eAED=0.39;_QI=652|0.75|0.8|1|0.25|0.4|5|302|95
chr5_2 exon 8534335 8534822 8534335 ID=Msa0754830-mRNA-1:exon:3917;Parent=Msa0754830-mRNA-1
chr5_2 exon 8535730 8535761 8535730 ID=Msa0754830-mRNA-1:exon:3916;Parent=Msa0754830-mRNA-1
chr5_2 exon 8536118 8536164 8536118 ID=Msa0754830-mRNA-1:exon:3915;Parent=Msa0754830-mRNA-1
chr5_2 exon 8537339 8537563 8537339 ID=Msa0754830-mRNA-1:exon:3914;Parent=Msa0754830-mRNA-1
chr5_2 exon 8537677 8538126 8537677 ID=Msa0754830-mRNA-1:exon:3913;Parent=Msa0754830-mRNA-1
chr5_2 five_prime_UTR 8537677 8538126 8537677 ID=Msa0754830-mRNA-1:five_prime_utr;Parent=Msa0754830-mRNA-1
chr5_2 five_prime_UTR 8537362 8537563 8537362 ID=Msa0754830-mRNA-1:five_prime_utr;Parent=Msa0754830-mRNA-1
chr5_2 CDS 8537339 8537361 8537339 ID=Msa0754830-mRNA-1:cds;Parent=Msa0754830-mRNA-1
chr5_2 CDS 8536118 8536164 8536118 ID=Msa0754830-mRNA-1:cds;Parent=Msa0754830-mRNA-1
chr5_2 CDS 8535730 8535761 8535730 ID=Msa0754830-mRNA-1:cds;Parent=Msa0754830-mRNA-1
chr5_2 CDS 8534637 8534822 8534637 ID=Msa0754830-mRNA-1:cds;Parent=Msa0754830-mRNA-1
chr5_2 three_prime_UTR 8534335 8534636 8534335 ID=Msa0754830-mRNA-1:three_prime_utr;Parent=Msa0754830-mRNA-1
Gene Sequence

>Msa0754830

ATGACTAATCGGATGGGAACAGGATTTATTCAAAGGAATGGAAATGAAGGGTTTATTAGATATGACTCAGGCTCAGAGTTGTTGATGAAGGTTAGGAATGAGTCTTTATCTGAGATAAAGTTGGAGCTAAAGAACAAGCAGAGCGACCAAGAGATAAAGTTCAAGGGCAATGCCAAACAAAGACTTTTCTTGATTAATTCTATTGTAGGAGATAAAAAAAAACTTTTAGAGTTGATTCGTATCATATTCATTTTATTTATTATTATTTTGACAAGAGGGTTGAATTAG
Protein sequence

>Msa0754830

MTNRMGTGFIQRNGNEGFIRYDSGSELLMKVRNESLSEIKLELKNKQSDQEIKFKGNAKQRLFLINSIVGDKKKLLELIRIIFILFIIILTRGLN*