AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0755350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755350 MtrunA17_Chr5g0400201 99.275 138 1 0 1 138 1 138 2.84e-96 273
Msa0755350 MtrunA17_Chr7g0266711 53.097 113 53 0 23 135 1 113 1.94e-36 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755350 AT1G32410.3 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755350 AT1G32410.4 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755350 AT1G32410.1 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755350 AT1G32410.2 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755350 AT1G32410.5 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755350 AT3G11530.1 49.558 113 57 0 23 135 1 113 7.32e-35 117
Msa0755350 AT3G11530.2 49.558 113 57 0 23 135 1 113 7.32e-35 117
Msa0755350 AT3G11530.3 45.652 92 50 0 44 135 1 92 7.96e-23 86.3

Find 58 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCAAAAGCAAACAATTTA+TGG 0.117700 5_2:+9112593 None:intergenic
AGGAATCGTCTTACAAATTT+TGG 0.200860 5_2:-9113495 Msa0755350:intron
ATGCCGTTGCTGTTCTTTGC+TGG 0.234696 5_2:-9113173 Msa0755350:CDS
TTCAATAAGGTTTGCATCAT+TGG 0.243794 5_2:-9112718 Msa0755350:three_prime_UTR
TATGAAACAGGAGTTCAATA+AGG 0.353730 5_2:-9112731 Msa0755350:exon
CTGTTCTTTGCTGGGTCTGA+TGG 0.357081 5_2:-9113164 Msa0755350:CDS
TGCCGTTGCTGTTCTTTGCT+GGG 0.388184 5_2:-9113172 Msa0755350:CDS
AATTTCGGCAAGTTCTTAAC+CGG 0.397297 5_2:-9112912 Msa0755350:CDS
CATCCACAGGAAACACAATA+TGG 0.410235 5_2:+9112779 None:intergenic
CTCTGTAGAAACACAGCTAT+TGG 0.410517 5_2:-9112508 Msa0755350:three_prime_UTR
CATGTGCTTTGTATAATAAT+TGG 0.413950 5_2:-9113365 Msa0755350:CDS
TGGTGCAGCTGGGTGAATTT+CGG 0.434061 5_2:-9112927 Msa0755350:intron
GTTACAGTATGCTATGAAAC+AGG 0.435514 5_2:-9112743 Msa0755350:exon
GAAGAACAGAGGTGGAAAGG+TGG 0.435810 5_2:+9112542 None:intergenic
CGGTTGGGCAGCCTTTGCAT+TGG 0.450746 5_2:-9112832 Msa0755350:CDS
CAGAGGTGGAAAGGTGGAGA+AGG 0.452632 5_2:+9112548 None:intergenic
AGTGAAGAACAGAGGTGGAA+AGG 0.471092 5_2:+9112539 None:intergenic
CCAGCATGTATTAATATGCT+TGG 0.485609 5_2:+9112861 None:intergenic
AATTGGTGGCCGATGCTTAG+TGG 0.489280 5_2:-9113348 Msa0755350:intron
AGAAATCAATAGCACGCAAA+AGG 0.498561 5_2:+9112677 None:intergenic
TTTATCAATATTTGAACTGA+GGG 0.500578 5_2:+9112622 None:intergenic
ATTGAGCAGCACTCTTGTAA+TGG 0.507513 5_2:+9112482 None:intergenic
TACATGCTGGTGTGATCGGT+TGG 0.512206 5_2:-9112848 Msa0755350:CDS
GTGGAAAGGTGGAGAAGGAG+AGG 0.513270 5_2:+9112553 None:intergenic
GCCTCCGAGAGTTGAGGCTC+CGG 0.515791 5_2:+9112893 None:intergenic
GCAAAGAACAGCAACGGCAT+TGG 0.515874 5_2:+9113176 None:intergenic
CCAACGTAGCGGTCCAACCT+TGG 0.516658 5_2:+9114774 None:intergenic
ATATTTGAACTGAGGGGAGG+AGG 0.528595 5_2:+9112629 None:intergenic
TGGAAGATGGATGACATACC+AGG 0.532972 5_2:-9113578 Msa0755350:exon
AAACAGTAACGTACATATCC+TGG 0.534955 5_2:+9113560 None:intergenic
ACAGAGTATCCTTCCAAGGT+TGG 0.538163 5_2:-9114787 Msa0755350:five_prime_UTR
ATGTTTAGAGTGAAGAACAG+AGG 0.550751 5_2:+9112531 None:intergenic
AGAAGAGAGTTCCAATGCAA+AGG 0.555565 5_2:+9112821 None:intergenic
CCAAGCATATTAATACATGC+TGG 0.560109 5_2:-9112861 Msa0755350:CDS
TACGTTACTGTTTCAACACA+GGG 0.563039 5_2:-9113550 Msa0755350:CDS
CGTAGCGGTCCAACCTTGGA+AGG 0.564278 5_2:+9114778 None:intergenic
ACATGCTGGTGTGATCGGTT+GGG 0.569252 5_2:-9112847 Msa0755350:CDS
TTAACCGGAGCCTCAACTCT+CGG 0.570335 5_2:-9112897 Msa0755350:CDS
TTTAGAGTGAAGAACAGAGG+TGG 0.584551 5_2:+9112534 None:intergenic
TTAATACATGCTGGTGTGAT+CGG 0.586015 5_2:-9112852 Msa0755350:CDS
TATCTTCATCACTCATCCAC+AGG 0.587041 5_2:+9112766 None:intergenic
AACATGAGGTGACTGGAAGA+TGG 0.587821 5_2:-9113591 Msa0755350:exon
GTTTATCAATATTTGAACTG+AGG 0.588641 5_2:+9112621 None:intergenic
TTTATCTTACCACTAAGCAT+CGG 0.589779 5_2:+9113339 None:intergenic
CGCAATGCCTCCGAGAGTTG+AGG 0.595677 5_2:+9112887 None:intergenic
TAGCCATATTGTGTTTCCTG+TGG 0.596306 5_2:-9112782 Msa0755350:CDS
GTGCTTTGTATAATAATTGG+TGG 0.599825 5_2:-9113362 Msa0755350:CDS
GTACGTTACTGTTTCAACAC+AGG 0.600532 5_2:-9113551 Msa0755350:CDS
TCAATATTTGAACTGAGGGG+AGG 0.603101 5_2:+9112626 None:intergenic
GACCCAGCAAAGAACAGCAA+CGG 0.621058 5_2:+9113170 None:intergenic
GAGGACAGAGTATCCTTCCA+AGG 0.630680 5_2:-9114791 Msa0755350:five_prime_UTR
GGAGAAAGTAAGGTGAGGCG+AGG 0.650011 5_2:-9114810 Msa0755350:five_prime_UTR
TCTCTGTAACATGAGGTGAC+TGG 0.685080 5_2:-9113598 Msa0755350:five_prime_UTR
ACCGGAGCCTCAACTCTCGG+AGG 0.710836 5_2:-9112894 Msa0755350:CDS
TTATCAATATTTGAACTGAG+GGG 0.710943 5_2:+9112623 None:intergenic
TGAATGGAGAAAGTAAGGTG+AGG 0.711315 5_2:-9114815 Msa0755350:five_prime_UTR
TGCTTGCTCTCTGTAACATG+AGG 0.711732 5_2:-9113605 Msa0755350:five_prime_UTR
AGATACATCAACCAACGTAG+CGG 0.730043 5_2:+9114763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATATCATTGATTTAT+TGG - chr5_2:9113132-9113151 Msa0755350:intron 15.0%
!!! TTTTGATATCATTGATTTAT+TGG - chr5_2:9113132-9113151 Msa0755350:intron 15.0%
!!! ATTCTTTTGCTTTTCATTTT+AGG - chr5_2:9112738-9112757 Msa0755350:exon 20.0%
!! ATTAAAAGCAATATGATCTT+AGG + chr5_2:9113072-9113091 None:intergenic 20.0%
!!! TAAGATCATATTGCTTTTAA+TGG - chr5_2:9113071-9113090 Msa0755350:intron 20.0%
!! TATGATTTGAATGTGATATA+GGG - chr5_2:9113241-9113260 Msa0755350:intron 20.0%
!! ATAGAGAACTTATGAAAATA+AGG + chr5_2:9113374-9113393 None:intergenic 20.0%
!! TAAAGTTACTTATATAACAC+AGG + chr5_2:9113452-9113471 None:intergenic 20.0%
!! TCTCTTTATGAATAAAATCA+TGG + chr5_2:9113831-9113850 None:intergenic 20.0%
!!! AGTTTAAATGTTTTCTTTGA+TGG - chr5_2:9113999-9114018 Msa0755350:intron 20.0%
!!! TAATAGGTAAGCTTTTATTT+TGG - chr5_2:9114167-9114186 Msa0755350:intron 20.0%
!! TTTATCAATATTTGAACTGA+GGG + chr5_2:9114665-9114684 None:intergenic 20.0%
!!! ATTCTTTTGCTTTTCATTTT+AGG - chr5_2:9112738-9112757 Msa0755350:exon 20.0%
!! ATTAAAAGCAATATGATCTT+AGG + chr5_2:9113072-9113091 None:intergenic 20.0%
!!! TAAGATCATATTGCTTTTAA+TGG - chr5_2:9113071-9113090 Msa0755350:intron 20.0%
!! TATGATTTGAATGTGATATA+GGG - chr5_2:9113241-9113260 Msa0755350:intron 20.0%
!! ATAGAGAACTTATGAAAATA+AGG + chr5_2:9113374-9113393 None:intergenic 20.0%
!! TAAAGTTACTTATATAACAC+AGG + chr5_2:9113452-9113471 None:intergenic 20.0%
!! TCTCTTTATGAATAAAATCA+TGG + chr5_2:9113831-9113850 None:intergenic 20.0%
!!! AGTTTAAATGTTTTCTTTGA+TGG - chr5_2:9113999-9114018 Msa0755350:intron 20.0%
!!! TAATAGGTAAGCTTTTATTT+TGG - chr5_2:9114167-9114186 Msa0755350:intron 20.0%
!! TTTATCAATATTTGAACTGA+GGG + chr5_2:9114665-9114684 None:intergenic 20.0%
!!! AAAATTCGTTTTGAGTTTTC+AGG - chr5_2:9112599-9112618 Msa0755350:three_prime_UTR 25.0%
!!! AGGTGGTAAATTTTGAAAAT+TGG + chr5_2:9112627-9112646 None:intergenic 25.0%
! GAAAGATCAAATCGATAATA+AGG + chr5_2:9112647-9112666 None:intergenic 25.0%
!! CATCGTTTTATCCAATTATT+AGG - chr5_2:9112666-9112685 Msa0755350:three_prime_UTR 25.0%
! GTATGATTTGAATGTGATAT+AGG - chr5_2:9113240-9113259 Msa0755350:intron 25.0%
!! TATTTGCTGACATAATCATT+TGG - chr5_2:9113290-9113309 Msa0755350:intron 25.0%
! GTATTAATTATTGCCCTATT+TGG + chr5_2:9113504-9113523 None:intergenic 25.0%
! ACAATGAAAAACTTTGAACA+TGG - chr5_2:9113522-9113541 Msa0755350:CDS 25.0%
! ATTAAATCAGAAGCAAATAC+TGG + chr5_2:9113612-9113631 None:intergenic 25.0%
! GAAAAAAGTTGACAACTATT+TGG - chr5_2:9113849-9113868 Msa0755350:intron 25.0%
! CATGTGCTTTGTATAATAAT+TGG - chr5_2:9113919-9113938 Msa0755350:intron 25.0%
!! TGTTTTCTGAATCTGATAAT+AGG - chr5_2:9114151-9114170 Msa0755350:intron 25.0%
! TTATCAATATTTGAACTGAG+GGG + chr5_2:9114664-9114683 None:intergenic 25.0%
! GTTTATCAATATTTGAACTG+AGG + chr5_2:9114666-9114685 None:intergenic 25.0%
!!! AAAATTCGTTTTGAGTTTTC+AGG - chr5_2:9112599-9112618 Msa0755350:three_prime_UTR 25.0%
!!! AGGTGGTAAATTTTGAAAAT+TGG + chr5_2:9112627-9112646 None:intergenic 25.0%
! GAAAGATCAAATCGATAATA+AGG + chr5_2:9112647-9112666 None:intergenic 25.0%
!! CATCGTTTTATCCAATTATT+AGG - chr5_2:9112666-9112685 Msa0755350:three_prime_UTR 25.0%
! GTATGATTTGAATGTGATAT+AGG - chr5_2:9113240-9113259 Msa0755350:intron 25.0%
!! TATTTGCTGACATAATCATT+TGG - chr5_2:9113290-9113309 Msa0755350:intron 25.0%
! GTATTAATTATTGCCCTATT+TGG + chr5_2:9113504-9113523 None:intergenic 25.0%
! ACAATGAAAAACTTTGAACA+TGG - chr5_2:9113522-9113541 Msa0755350:CDS 25.0%
! ATTAAATCAGAAGCAAATAC+TGG + chr5_2:9113612-9113631 None:intergenic 25.0%
! GAAAAAAGTTGACAACTATT+TGG - chr5_2:9113849-9113868 Msa0755350:intron 25.0%
! CATGTGCTTTGTATAATAAT+TGG - chr5_2:9113919-9113938 Msa0755350:intron 25.0%
!! TGTTTTCTGAATCTGATAAT+AGG - chr5_2:9114151-9114170 Msa0755350:intron 25.0%
! TTATCAATATTTGAACTGAG+GGG + chr5_2:9114664-9114683 None:intergenic 25.0%
! GTTTATCAATATTTGAACTG+AGG + chr5_2:9114666-9114685 None:intergenic 25.0%
!!! AAGTGAAACGTTTTTTGTGA+GGG + chr5_2:9112569-9112588 None:intergenic 30.0%
AGATCAAATCGATAATAAGG+TGG + chr5_2:9112644-9112663 None:intergenic 30.0%
ACATACAGCAACCTAATAAT+TGG + chr5_2:9112680-9112699 None:intergenic 30.0%
TACAAGATAAACGAGATTGA+AGG + chr5_2:9112802-9112821 None:intergenic 30.0%
GAATTGATTGAATCGTGATT+TGG + chr5_2:9112827-9112846 None:intergenic 30.0%
ATATGATCTTAGGCTCTATT+TGG + chr5_2:9113062-9113081 None:intergenic 30.0%
AACAAGTTGCTTATCCAAAT+AGG - chr5_2:9113487-9113506 Msa0755350:intron 30.0%
ACAAGTTGCTTATCCAAATA+GGG - chr5_2:9113488-9113507 Msa0755350:intron 30.0%
AGATGAAGTCAAAACAAAAG+TGG + chr5_2:9113552-9113571 None:intergenic 30.0%
! AGGAATCGTCTTACAAATTT+TGG - chr5_2:9113789-9113808 Msa0755350:intron 30.0%
!! ATCGTCTTACAAATTTTGGT+CGG - chr5_2:9113793-9113812 Msa0755350:intron 30.0%
GTGCTTTGTATAATAATTGG+TGG - chr5_2:9113922-9113941 Msa0755350:intron 30.0%
TTTATCTTACCACTAAGCAT+CGG + chr5_2:9113948-9113967 None:intergenic 30.0%
! TTTCTTTGATGGCATTGTTA+CGG - chr5_2:9114010-9114029 Msa0755350:intron 30.0%
!! AAGATCTGAATGCTTTAGAA+AGG - chr5_2:9114261-9114280 Msa0755350:intron 30.0%
TAATCACTCATCTCAAGATA+TGG + chr5_2:9114301-9114320 None:intergenic 30.0%
!!! TTGTTTTGTGATTTGCTGAA+TGG - chr5_2:9114334-9114353 Msa0755350:intron 30.0%
TATGAAACAGGAGTTCAATA+AGG - chr5_2:9114553-9114572 Msa0755350:intron 30.0%
TTCAATAAGGTTTGCATCAT+TGG - chr5_2:9114566-9114585 Msa0755350:intron 30.0%
ACGCAAAAGCAAACAATTTA+TGG + chr5_2:9114694-9114713 None:intergenic 30.0%
!!! AAGTGAAACGTTTTTTGTGA+GGG + chr5_2:9112569-9112588 None:intergenic 30.0%
AGATCAAATCGATAATAAGG+TGG + chr5_2:9112644-9112663 None:intergenic 30.0%
ACATACAGCAACCTAATAAT+TGG + chr5_2:9112680-9112699 None:intergenic 30.0%
TACAAGATAAACGAGATTGA+AGG + chr5_2:9112802-9112821 None:intergenic 30.0%
GAATTGATTGAATCGTGATT+TGG + chr5_2:9112827-9112846 None:intergenic 30.0%
ATATGATCTTAGGCTCTATT+TGG + chr5_2:9113062-9113081 None:intergenic 30.0%
AACAAGTTGCTTATCCAAAT+AGG - chr5_2:9113487-9113506 Msa0755350:intron 30.0%
ACAAGTTGCTTATCCAAATA+GGG - chr5_2:9113488-9113507 Msa0755350:intron 30.0%
AGATGAAGTCAAAACAAAAG+TGG + chr5_2:9113552-9113571 None:intergenic 30.0%
! AGGAATCGTCTTACAAATTT+TGG - chr5_2:9113789-9113808 Msa0755350:intron 30.0%
!! ATCGTCTTACAAATTTTGGT+CGG - chr5_2:9113793-9113812 Msa0755350:intron 30.0%
GTGCTTTGTATAATAATTGG+TGG - chr5_2:9113922-9113941 Msa0755350:intron 30.0%
TTTATCTTACCACTAAGCAT+CGG + chr5_2:9113948-9113967 None:intergenic 30.0%
! TTTCTTTGATGGCATTGTTA+CGG - chr5_2:9114010-9114029 Msa0755350:intron 30.0%
!! AAGATCTGAATGCTTTAGAA+AGG - chr5_2:9114261-9114280 Msa0755350:intron 30.0%
TAATCACTCATCTCAAGATA+TGG + chr5_2:9114301-9114320 None:intergenic 30.0%
!!! TTGTTTTGTGATTTGCTGAA+TGG - chr5_2:9114334-9114353 Msa0755350:intron 30.0%
TATGAAACAGGAGTTCAATA+AGG - chr5_2:9114553-9114572 Msa0755350:intron 30.0%
TTCAATAAGGTTTGCATCAT+TGG - chr5_2:9114566-9114585 Msa0755350:intron 30.0%
ACGCAAAAGCAAACAATTTA+TGG + chr5_2:9114694-9114713 None:intergenic 30.0%
! TTTTTGTGAGGGTTACTTCT+TGG + chr5_2:9112558-9112577 None:intergenic 35.0%
!!! GAAGTGAAACGTTTTTTGTG+AGG + chr5_2:9112570-9112589 None:intergenic 35.0%
ACAAATAAGCCAATCCAAAC+AGG + chr5_2:9113267-9113286 None:intergenic 35.0%
! AATCATTTGGAACACTGTCT+AGG - chr5_2:9113303-9113322 Msa0755350:intron 35.0%
AAACAGTAACGTACATATCC+TGG + chr5_2:9113727-9113746 None:intergenic 35.0%
TACGTTACTGTTTCAACACA+GGG - chr5_2:9113734-9113753 Msa0755350:intron 35.0%
! AACTTGTGTTTTGTGTTGTC+AGG - chr5_2:9113896-9113915 Msa0755350:intron 35.0%
CATTTAAACTTGCAGTGTAC+TGG + chr5_2:9113989-9114008 None:intergenic 35.0%
! TTCTTTGATGGCATTGTTAC+GGG - chr5_2:9114011-9114030 Msa0755350:intron 35.0%
ATGTAATAACCACCAACCAA+GGG + chr5_2:9114046-9114065 None:intergenic 35.0%
AATGTAATAACCACCAACCA+AGG + chr5_2:9114047-9114066 None:intergenic 35.0%
!! TGGTTGGTGGTTATTACATT+CGG - chr5_2:9114047-9114066 Msa0755350:intron 35.0%
!!! TTTTGTGATTTGCTGAATGG+TGG - chr5_2:9114337-9114356 Msa0755350:intron 35.0%
AATTTCGGCAAGTTCTTAAC+CGG - chr5_2:9114372-9114391 Msa0755350:intron 35.0%
CCAGCATGTATTAATATGCT+TGG + chr5_2:9114426-9114445 None:intergenic 35.0%
CCAAGCATATTAATACATGC+TGG - chr5_2:9114423-9114442 Msa0755350:intron 35.0%
TTAATACATGCTGGTGTGAT+CGG - chr5_2:9114432-9114451 Msa0755350:intron 35.0%
GTTACAGTATGCTATGAAAC+AGG - chr5_2:9114541-9114560 Msa0755350:intron 35.0%
AGAAATCAATAGCACGCAAA+AGG + chr5_2:9114610-9114629 None:intergenic 35.0%
! ATGTTTAGAGTGAAGAACAG+AGG + chr5_2:9114756-9114775 None:intergenic 35.0%
! TTTTTGTGAGGGTTACTTCT+TGG + chr5_2:9112558-9112577 None:intergenic 35.0%
!!! GAAGTGAAACGTTTTTTGTG+AGG + chr5_2:9112570-9112589 None:intergenic 35.0%
ACAAATAAGCCAATCCAAAC+AGG + chr5_2:9113267-9113286 None:intergenic 35.0%
! AATCATTTGGAACACTGTCT+AGG - chr5_2:9113303-9113322 Msa0755350:intron 35.0%
AAACAGTAACGTACATATCC+TGG + chr5_2:9113727-9113746 None:intergenic 35.0%
TACGTTACTGTTTCAACACA+GGG - chr5_2:9113734-9113753 Msa0755350:intron 35.0%
! AACTTGTGTTTTGTGTTGTC+AGG - chr5_2:9113896-9113915 Msa0755350:intron 35.0%
CATTTAAACTTGCAGTGTAC+TGG + chr5_2:9113989-9114008 None:intergenic 35.0%
! TTCTTTGATGGCATTGTTAC+GGG - chr5_2:9114011-9114030 Msa0755350:intron 35.0%
ATGTAATAACCACCAACCAA+GGG + chr5_2:9114046-9114065 None:intergenic 35.0%
AATGTAATAACCACCAACCA+AGG + chr5_2:9114047-9114066 None:intergenic 35.0%
!! TGGTTGGTGGTTATTACATT+CGG - chr5_2:9114047-9114066 Msa0755350:intron 35.0%
!!! TTTTGTGATTTGCTGAATGG+TGG - chr5_2:9114337-9114356 Msa0755350:intron 35.0%
AATTTCGGCAAGTTCTTAAC+CGG - chr5_2:9114372-9114391 Msa0755350:intron 35.0%
CCAGCATGTATTAATATGCT+TGG + chr5_2:9114426-9114445 None:intergenic 35.0%
CCAAGCATATTAATACATGC+TGG - chr5_2:9114423-9114442 Msa0755350:intron 35.0%
TTAATACATGCTGGTGTGAT+CGG - chr5_2:9114432-9114451 Msa0755350:intron 35.0%
GTTACAGTATGCTATGAAAC+AGG - chr5_2:9114541-9114560 Msa0755350:intron 35.0%
AGAAATCAATAGCACGCAAA+AGG + chr5_2:9114610-9114629 None:intergenic 35.0%
! ATGTTTAGAGTGAAGAACAG+AGG + chr5_2:9114756-9114775 None:intergenic 35.0%
TGAATGGAGAAAGTAAGGTG+AGG - chr5_2:9112469-9112488 Msa0755350:three_prime_UTR 40.0%
AGATACATCAACCAACGTAG+CGG + chr5_2:9112524-9112543 None:intergenic 40.0%
TGATTCACGACGATACAACA+TGG - chr5_2:9113097-9113116 Msa0755350:intron 40.0%
GTTCGTGATCTTAAGCTACT+AGG - chr5_2:9113166-9113185 Msa0755350:CDS 40.0%
! AGCTACTAGGTTTGCTTTCT+TGG - chr5_2:9113179-9113198 Msa0755350:CDS 40.0%
AATGTGATATAGGGCCTGTT+TGG - chr5_2:9113250-9113269 Msa0755350:intron 40.0%
TCGTAAGCACTAAGCTATGA+GGG + chr5_2:9113636-9113655 None:intergenic 40.0%
GTACGTTACTGTTTCAACAC+AGG - chr5_2:9113733-9113752 Msa0755350:intron 40.0%
!! GCTTCTCTTTTTGGCACTTT+TGG - chr5_2:9113759-9113778 Msa0755350:intron 40.0%
!! TTTTTGGCACTTTTGGTCTC+AGG - chr5_2:9113766-9113785 Msa0755350:intron 40.0%
! TCTTTGATGGCATTGTTACG+GGG - chr5_2:9114012-9114031 Msa0755350:intron 40.0%
TGTAATAACCACCAACCAAG+GGG + chr5_2:9114045-9114064 None:intergenic 40.0%
AGAAGAGAGTTCCAATGCAA+AGG + chr5_2:9114466-9114485 None:intergenic 40.0%
TAGCCATATTGTGTTTCCTG+TGG - chr5_2:9114502-9114521 Msa0755350:intron 40.0%
CATCCACAGGAAACACAATA+TGG + chr5_2:9114508-9114527 None:intergenic 40.0%
TATCTTCATCACTCATCCAC+AGG + chr5_2:9114521-9114540 None:intergenic 40.0%
TCAATATTTGAACTGAGGGG+AGG + chr5_2:9114661-9114680 None:intergenic 40.0%
! TTTAGAGTGAAGAACAGAGG+TGG + chr5_2:9114753-9114772 None:intergenic 40.0%
CTCTGTAGAAACACAGCTAT+TGG - chr5_2:9114776-9114795 Msa0755350:five_prime_UTR 40.0%
ATTGAGCAGCACTCTTGTAA+TGG + chr5_2:9114805-9114824 None:intergenic 40.0%
TGAATGGAGAAAGTAAGGTG+AGG - chr5_2:9112469-9112488 Msa0755350:three_prime_UTR 40.0%
AGATACATCAACCAACGTAG+CGG + chr5_2:9112524-9112543 None:intergenic 40.0%
TGATTCACGACGATACAACA+TGG - chr5_2:9113097-9113116 Msa0755350:intron 40.0%
GTTCGTGATCTTAAGCTACT+AGG - chr5_2:9113166-9113185 Msa0755350:CDS 40.0%
! AGCTACTAGGTTTGCTTTCT+TGG - chr5_2:9113179-9113198 Msa0755350:CDS 40.0%
AATGTGATATAGGGCCTGTT+TGG - chr5_2:9113250-9113269 Msa0755350:intron 40.0%
TCGTAAGCACTAAGCTATGA+GGG + chr5_2:9113636-9113655 None:intergenic 40.0%
GTACGTTACTGTTTCAACAC+AGG - chr5_2:9113733-9113752 Msa0755350:intron 40.0%
!! GCTTCTCTTTTTGGCACTTT+TGG - chr5_2:9113759-9113778 Msa0755350:intron 40.0%
!! TTTTTGGCACTTTTGGTCTC+AGG - chr5_2:9113766-9113785 Msa0755350:intron 40.0%
! TCTTTGATGGCATTGTTACG+GGG - chr5_2:9114012-9114031 Msa0755350:intron 40.0%
TGTAATAACCACCAACCAAG+GGG + chr5_2:9114045-9114064 None:intergenic 40.0%
AGAAGAGAGTTCCAATGCAA+AGG + chr5_2:9114466-9114485 None:intergenic 40.0%
TAGCCATATTGTGTTTCCTG+TGG - chr5_2:9114502-9114521 Msa0755350:intron 40.0%
CATCCACAGGAAACACAATA+TGG + chr5_2:9114508-9114527 None:intergenic 40.0%
TATCTTCATCACTCATCCAC+AGG + chr5_2:9114521-9114540 None:intergenic 40.0%
TCAATATTTGAACTGAGGGG+AGG + chr5_2:9114661-9114680 None:intergenic 40.0%
! TTTAGAGTGAAGAACAGAGG+TGG + chr5_2:9114753-9114772 None:intergenic 40.0%
CTCTGTAGAAACACAGCTAT+TGG - chr5_2:9114776-9114795 Msa0755350:five_prime_UTR 40.0%
ATTGAGCAGCACTCTTGTAA+TGG + chr5_2:9114805-9114824 None:intergenic 40.0%
! ACAGAGTATCCTTCCAAGGT+TGG - chr5_2:9112497-9112516 Msa0755350:three_prime_UTR 45.0%
CTCACGAGTGATTATGTGAG+TGG + chr5_2:9112857-9112876 None:intergenic 45.0%
! TGCTTTCTTGGTGCATAGAC+AGG - chr5_2:9113191-9113210 Msa0755350:CDS 45.0%
GATATAGGGCCTGTTTGGAT+TGG - chr5_2:9113255-9113274 Msa0755350:intron 45.0%
CTCGTAAGCACTAAGCTATG+AGG + chr5_2:9113637-9113656 None:intergenic 45.0%
TGCTTGCTCTCTGTAACATG+AGG - chr5_2:9113679-9113698 Msa0755350:intron 45.0%
TCTCTGTAACATGAGGTGAC+TGG - chr5_2:9113686-9113705 Msa0755350:intron 45.0%
AACATGAGGTGACTGGAAGA+TGG - chr5_2:9113693-9113712 Msa0755350:intron 45.0%
TGGAAGATGGATGACATACC+AGG - chr5_2:9113706-9113725 Msa0755350:intron 45.0%
! CACAGGGAAGCTTCTCTTTT+TGG - chr5_2:9113750-9113769 Msa0755350:intron 45.0%
ATATTTGAACTGAGGGGAGG+AGG + chr5_2:9114658-9114677 None:intergenic 45.0%
AGTGAAGAACAGAGGTGGAA+AGG + chr5_2:9114748-9114767 None:intergenic 45.0%
! ACAGAGTATCCTTCCAAGGT+TGG - chr5_2:9112497-9112516 Msa0755350:three_prime_UTR 45.0%
CTCACGAGTGATTATGTGAG+TGG + chr5_2:9112857-9112876 None:intergenic 45.0%
! TGCTTTCTTGGTGCATAGAC+AGG - chr5_2:9113191-9113210 Msa0755350:CDS 45.0%
GATATAGGGCCTGTTTGGAT+TGG - chr5_2:9113255-9113274 Msa0755350:intron 45.0%
CTCGTAAGCACTAAGCTATG+AGG + chr5_2:9113637-9113656 None:intergenic 45.0%
TGCTTGCTCTCTGTAACATG+AGG - chr5_2:9113679-9113698 Msa0755350:intron 45.0%
TCTCTGTAACATGAGGTGAC+TGG - chr5_2:9113686-9113705 Msa0755350:intron 45.0%
AACATGAGGTGACTGGAAGA+TGG - chr5_2:9113693-9113712 Msa0755350:intron 45.0%
TGGAAGATGGATGACATACC+AGG - chr5_2:9113706-9113725 Msa0755350:intron 45.0%
! CACAGGGAAGCTTCTCTTTT+TGG - chr5_2:9113750-9113769 Msa0755350:intron 45.0%
ATATTTGAACTGAGGGGAGG+AGG + chr5_2:9114658-9114677 None:intergenic 45.0%
AGTGAAGAACAGAGGTGGAA+AGG + chr5_2:9114748-9114767 None:intergenic 45.0%
! GAGGACAGAGTATCCTTCCA+AGG - chr5_2:9112493-9112512 Msa0755350:three_prime_UTR 50.0%
! TTGGCACTTTTGGTCTCAGG+AGG - chr5_2:9113769-9113788 Msa0755350:intron 50.0%
! AATTGGTGGCCGATGCTTAG+TGG - chr5_2:9113936-9113955 Msa0755350:intron 50.0%
TTACGGGGAGAAAATCCCCT+TGG - chr5_2:9114027-9114046 Msa0755350:intron 50.0%
GAGAAAATCCCCTTGGTTGG+TGG - chr5_2:9114034-9114053 Msa0755350:intron 50.0%
GCAAAGAACAGCAACGGCAT+TGG + chr5_2:9114111-9114130 None:intergenic 50.0%
! ATGCCGTTGCTGTTCTTTGC+TGG - chr5_2:9114111-9114130 Msa0755350:intron 50.0%
! TGCCGTTGCTGTTCTTTGCT+GGG - chr5_2:9114112-9114131 Msa0755350:intron 50.0%
GACCCAGCAAAGAACAGCAA+CGG + chr5_2:9114117-9114136 None:intergenic 50.0%
CTGTTCTTTGCTGGGTCTGA+TGG - chr5_2:9114120-9114139 Msa0755350:intron 50.0%
TGGTGCAGCTGGGTGAATTT+CGG - chr5_2:9114357-9114376 Msa0755350:intron 50.0%
TTAACCGGAGCCTCAACTCT+CGG - chr5_2:9114387-9114406 Msa0755350:intron 50.0%
TACATGCTGGTGTGATCGGT+TGG - chr5_2:9114436-9114455 Msa0755350:intron 50.0%
ACATGCTGGTGTGATCGGTT+GGG - chr5_2:9114437-9114456 Msa0755350:intron 50.0%
GAAGAACAGAGGTGGAAAGG+TGG + chr5_2:9114745-9114764 None:intergenic 50.0%
! GAGGACAGAGTATCCTTCCA+AGG - chr5_2:9112493-9112512 Msa0755350:three_prime_UTR 50.0%
! TTGGCACTTTTGGTCTCAGG+AGG - chr5_2:9113769-9113788 Msa0755350:intron 50.0%
! AATTGGTGGCCGATGCTTAG+TGG - chr5_2:9113936-9113955 Msa0755350:intron 50.0%
TTACGGGGAGAAAATCCCCT+TGG - chr5_2:9114027-9114046 Msa0755350:intron 50.0%
GAGAAAATCCCCTTGGTTGG+TGG - chr5_2:9114034-9114053 Msa0755350:intron 50.0%
GCAAAGAACAGCAACGGCAT+TGG + chr5_2:9114111-9114130 None:intergenic 50.0%
! ATGCCGTTGCTGTTCTTTGC+TGG - chr5_2:9114111-9114130 Msa0755350:intron 50.0%
! TGCCGTTGCTGTTCTTTGCT+GGG - chr5_2:9114112-9114131 Msa0755350:intron 50.0%
GACCCAGCAAAGAACAGCAA+CGG + chr5_2:9114117-9114136 None:intergenic 50.0%
CTGTTCTTTGCTGGGTCTGA+TGG - chr5_2:9114120-9114139 Msa0755350:intron 50.0%
TGGTGCAGCTGGGTGAATTT+CGG - chr5_2:9114357-9114376 Msa0755350:intron 50.0%
TTAACCGGAGCCTCAACTCT+CGG - chr5_2:9114387-9114406 Msa0755350:intron 50.0%
TACATGCTGGTGTGATCGGT+TGG - chr5_2:9114436-9114455 Msa0755350:intron 50.0%
ACATGCTGGTGTGATCGGTT+GGG - chr5_2:9114437-9114456 Msa0755350:intron 50.0%
GAAGAACAGAGGTGGAAAGG+TGG + chr5_2:9114745-9114764 None:intergenic 50.0%
GGAGAAAGTAAGGTGAGGCG+AGG - chr5_2:9112474-9112493 Msa0755350:three_prime_UTR 55.0%
GGGGAGAAAATCCCCTTGGT+TGG - chr5_2:9114031-9114050 Msa0755350:intron 55.0%
! TTGCTGAATGGTGGTGCAGC+TGG - chr5_2:9114346-9114365 Msa0755350:intron 55.0%
! TGCTGAATGGTGGTGCAGCT+GGG - chr5_2:9114347-9114366 Msa0755350:intron 55.0%
GTGGAAAGGTGGAGAAGGAG+AGG + chr5_2:9114734-9114753 None:intergenic 55.0%
CAGAGGTGGAAAGGTGGAGA+AGG + chr5_2:9114739-9114758 None:intergenic 55.0%
GGAGAAAGTAAGGTGAGGCG+AGG - chr5_2:9112474-9112493 Msa0755350:three_prime_UTR 55.0%
GGGGAGAAAATCCCCTTGGT+TGG - chr5_2:9114031-9114050 Msa0755350:intron 55.0%
! TTGCTGAATGGTGGTGCAGC+TGG - chr5_2:9114346-9114365 Msa0755350:intron 55.0%
! TGCTGAATGGTGGTGCAGCT+GGG - chr5_2:9114347-9114366 Msa0755350:intron 55.0%
GTGGAAAGGTGGAGAAGGAG+AGG + chr5_2:9114734-9114753 None:intergenic 55.0%
CAGAGGTGGAAAGGTGGAGA+AGG + chr5_2:9114739-9114758 None:intergenic 55.0%
CGTAGCGGTCCAACCTTGGA+AGG + chr5_2:9112509-9112528 None:intergenic 60.0%
CCAACGTAGCGGTCCAACCT+TGG + chr5_2:9112513-9112532 None:intergenic 60.0%
CCAAGGTTGGACCGCTACGT+TGG - chr5_2:9112510-9112529 Msa0755350:three_prime_UTR 60.0%
CGCAATGCCTCCGAGAGTTG+AGG + chr5_2:9114400-9114419 None:intergenic 60.0%
CGGTTGGGCAGCCTTTGCAT+TGG - chr5_2:9114452-9114471 Msa0755350:intron 60.0%
CGTAGCGGTCCAACCTTGGA+AGG + chr5_2:9112509-9112528 None:intergenic 60.0%
CCAACGTAGCGGTCCAACCT+TGG + chr5_2:9112513-9112532 None:intergenic 60.0%
CCAAGGTTGGACCGCTACGT+TGG - chr5_2:9112510-9112529 Msa0755350:three_prime_UTR 60.0%
CGCAATGCCTCCGAGAGTTG+AGG + chr5_2:9114400-9114419 None:intergenic 60.0%
CGGTTGGGCAGCCTTTGCAT+TGG - chr5_2:9114452-9114471 Msa0755350:intron 60.0%
ACCGGAGCCTCAACTCTCGG+AGG - chr5_2:9114390-9114409 Msa0755350:intron 65.0%
GCCTCCGAGAGTTGAGGCTC+CGG + chr5_2:9114394-9114413 None:intergenic 65.0%
ACCGGAGCCTCAACTCTCGG+AGG - chr5_2:9114390-9114409 Msa0755350:intron 65.0%
GCCTCCGAGAGTTGAGGCTC+CGG + chr5_2:9114394-9114413 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 9112469 9114837 9112469 ID=Msa0755350;Name=Msa0755350
chr5_2 mRNA 9112469 9114837 9112469 ID=Msa0755350-mRNA-1;Parent=Msa0755350;Name=Msa0755350-mRNA-1;_AED=0.02;_eAED=0.02;_QI=196|1|1|1|0.75|0.6|5|280|138
chr5_2 exon 9112469 9112941 9112469 ID=Msa0755350-mRNA-1:exon:4258;Parent=Msa0755350-mRNA-1
chr5_2 exon 9113134 9113218 9113134 ID=Msa0755350-mRNA-1:exon:4257;Parent=Msa0755350-mRNA-1
chr5_2 exon 9113349 9113388 9113349 ID=Msa0755350-mRNA-1:exon:4256;Parent=Msa0755350-mRNA-1
chr5_2 exon 9113496 9113638 9113496 ID=Msa0755350-mRNA-1:exon:4255;Parent=Msa0755350-mRNA-1
chr5_2 exon 9114686 9114837 9114686 ID=Msa0755350-mRNA-1:exon:4254;Parent=Msa0755350-mRNA-1
chr5_2 five_prime_UTR 9114686 9114837 9114686 ID=Msa0755350-mRNA-1:five_prime_utr;Parent=Msa0755350-mRNA-1
chr5_2 five_prime_UTR 9113595 9113638 9113595 ID=Msa0755350-mRNA-1:five_prime_utr;Parent=Msa0755350-mRNA-1
chr5_2 CDS 9113496 9113594 9113496 ID=Msa0755350-mRNA-1:cds;Parent=Msa0755350-mRNA-1
chr5_2 CDS 9113349 9113388 9113349 ID=Msa0755350-mRNA-1:cds;Parent=Msa0755350-mRNA-1
chr5_2 CDS 9113134 9113218 9113134 ID=Msa0755350-mRNA-1:cds;Parent=Msa0755350-mRNA-1
chr5_2 CDS 9112749 9112941 9112749 ID=Msa0755350-mRNA-1:cds;Parent=Msa0755350-mRNA-1
chr5_2 three_prime_UTR 9112469 9112748 9112469 ID=Msa0755350-mRNA-1:three_prime_utr;Parent=Msa0755350-mRNA-1
Gene Sequence

>Msa0755350

ATGGATGACATACCAGGATATGTACGTTACTGTTTCAACACAGGGAAGCTTCTCTTTTTGGCACTTTTGGTCTCAGGAGGAATCGTCTTACAAATTTTGGCATGTGCTTTGTATAATAATTGGTGGCCGATGCTTAGTGCTATAACGTATGTGCTTCTTCCAATGCCGTTGCTGTTCTTTGCTGGGTCTGATGGTTCTTCTATGTTTTCTGAATCTGATAATAGCTGGGTGAATTTCGGCAAGTTCTTAACCGGAGCCTCAACTCTCGGAGGCATTGCGATTCCAAGCATATTAATACATGCTGGTGTGATCGGTTGGGCAGCCTTTGCATTGGAACTCTCTTCTTTCTTTGTGTTTATATTAGCCATATTGTGTTTCCTGTGGATGAGTGATGAAGATAGTTACAGTATGCTATGA
Protein sequence

>Msa0755350

MDDIPGYVRYCFNTGKLLFLALLVSGGIVLQILACALYNNWWPMLSAITYVLLPMPLLFFAGSDGSSMFSESDNSWVNFGKFLTGASTLGGIAIPSILIHAGVIGWAAFALELSSFFVFILAILCFLWMSDEDSYSML*