AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0755170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755170 MtrunA17_Chr5g0400201 99.275 138 1 0 1 138 1 138 2.84e-96 273
Msa0755170 MtrunA17_Chr7g0266711 53.097 113 53 0 23 135 1 113 1.94e-36 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755170 AT1G32410.3 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755170 AT1G32410.4 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755170 AT1G32410.1 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755170 AT1G32410.2 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755170 AT1G32410.5 67.647 136 43 1 1 135 1 136 5.39e-63 189
Msa0755170 AT3G11530.1 49.558 113 57 0 23 135 1 113 7.32e-35 117
Msa0755170 AT3G11530.2 49.558 113 57 0 23 135 1 113 7.32e-35 117
Msa0755170 AT3G11530.3 45.652 92 50 0 44 135 1 92 7.96e-23 86.3

Find 59 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCAAAAGCAAACAATTTA+TGG 0.117700 5_2:+8956094 None:intergenic
AGGAATCGTCTTACAAATTT+TGG 0.200860 5_2:-8956996 Msa0755170:intron
ATGCCGTTGCTGTTCTTTGC+TGG 0.234696 5_2:-8956674 Msa0755170:CDS
TTCAATAAGGTTTGCATCAT+TGG 0.243794 5_2:-8956219 Msa0755170:three_prime_UTR
TATGAAACAGGAGTTCAATA+AGG 0.353730 5_2:-8956232 Msa0755170:exon
CTGTTCTTTGCTGGGTCTGA+TGG 0.357081 5_2:-8956665 Msa0755170:CDS
TGCCGTTGCTGTTCTTTGCT+GGG 0.388184 5_2:-8956673 Msa0755170:CDS
AATTTCGGCAAGTTCTTAAC+CGG 0.397297 5_2:-8956413 Msa0755170:CDS
CATCCACAGGAAACACAATA+TGG 0.410235 5_2:+8956280 None:intergenic
CTCTGTAGAAACACAGCTAT+TGG 0.410517 5_2:-8956009 Msa0755170:three_prime_UTR
CATGTGCTTTGTATAATAAT+TGG 0.413950 5_2:-8956866 Msa0755170:CDS
CAACGTAGCGGTCCAACCTT+TGG 0.423970 5_2:+8958276 None:intergenic
TGGTGCAGCTGGGTGAATTT+CGG 0.434061 5_2:-8956428 Msa0755170:intron
GTTACAGTATGCTATGAAAC+AGG 0.435514 5_2:-8956244 Msa0755170:exon
GAAGAACAGAGGTGGAAAGG+TGG 0.435810 5_2:+8956043 None:intergenic
CGGTTGGGCAGCCTTTGCAT+TGG 0.450746 5_2:-8956333 Msa0755170:CDS
CAGAGGTGGAAAGGTGGAGA+AGG 0.452632 5_2:+8956049 None:intergenic
AGTGAAGAACAGAGGTGGAA+AGG 0.471092 5_2:+8956040 None:intergenic
CCAGCATGTATTAATATGCT+TGG 0.485609 5_2:+8956362 None:intergenic
AATTGGTGGCCGATGCTTAG+TGG 0.489280 5_2:-8956849 Msa0755170:intron
AGAAATCAATAGCACGCAAA+AGG 0.498561 5_2:+8956178 None:intergenic
TTTATCAATATTTGAACTGA+GGG 0.500578 5_2:+8956123 None:intergenic
ATTGAGCAGCACTCTTGTAA+TGG 0.507513 5_2:+8955983 None:intergenic
TACATGCTGGTGTGATCGGT+TGG 0.512206 5_2:-8956349 Msa0755170:CDS
GTGGAAAGGTGGAGAAGGAG+AGG 0.513270 5_2:+8956054 None:intergenic
GCCTCCGAGAGTTGAGGCTC+CGG 0.515791 5_2:+8956394 None:intergenic
GCAAAGAACAGCAACGGCAT+TGG 0.515874 5_2:+8956677 None:intergenic
CAGAGTATCCTTCCAAAGGT+TGG 0.521179 5_2:-8958288 Msa0755170:five_prime_UTR
ATATTTGAACTGAGGGGAGG+AGG 0.528595 5_2:+8956130 None:intergenic
TGGAAGATGGATGACATACC+AGG 0.532972 5_2:-8957079 Msa0755170:exon
AAACAGTAACGTACATATCC+TGG 0.534955 5_2:+8957061 None:intergenic
ATGTTTAGAGTGAAGAACAG+AGG 0.550751 5_2:+8956032 None:intergenic
AGAAGAGAGTTCCAATGCAA+AGG 0.555565 5_2:+8956322 None:intergenic
CCAAGCATATTAATACATGC+TGG 0.560109 5_2:-8956362 Msa0755170:CDS
TACGTTACTGTTTCAACACA+GGG 0.563039 5_2:-8957051 Msa0755170:CDS
ACATGCTGGTGTGATCGGTT+GGG 0.569252 5_2:-8956348 Msa0755170:CDS
TTAACCGGAGCCTCAACTCT+CGG 0.570335 5_2:-8956398 Msa0755170:CDS
GGAGAAAGTAAGGTGAGGTG+AGG 0.578551 5_2:-8958312 Msa0755170:five_prime_UTR
TTTAGAGTGAAGAACAGAGG+TGG 0.584551 5_2:+8956035 None:intergenic
TTAATACATGCTGGTGTGAT+CGG 0.586015 5_2:-8956353 Msa0755170:CDS
TATCTTCATCACTCATCCAC+AGG 0.587041 5_2:+8956267 None:intergenic
AACATGAGGTGACTGGAAGA+TGG 0.587821 5_2:-8957092 Msa0755170:exon
GTTTATCAATATTTGAACTG+AGG 0.588641 5_2:+8956122 None:intergenic
TTTATCTTACCACTAAGCAT+CGG 0.589779 5_2:+8956840 None:intergenic
CGCAATGCCTCCGAGAGTTG+AGG 0.595677 5_2:+8956388 None:intergenic
TAGCCATATTGTGTTTCCTG+TGG 0.596306 5_2:-8956283 Msa0755170:CDS
GTGCTTTGTATAATAATTGG+TGG 0.599825 5_2:-8956863 Msa0755170:CDS
GTACGTTACTGTTTCAACAC+AGG 0.600532 5_2:-8957052 Msa0755170:CDS
CAAAGGTTGGACCGCTACGT+TGG 0.600699 5_2:-8958275 Msa0755170:five_prime_UTR
TCAATATTTGAACTGAGGGG+AGG 0.603101 5_2:+8956127 None:intergenic
GACCCAGCAAAGAACAGCAA+CGG 0.621058 5_2:+8956671 None:intergenic
GTAGCGGTCCAACCTTTGGA+AGG 0.629181 5_2:+8958280 None:intergenic
AGGACAGAGTATCCTTCCAA+AGG 0.640964 5_2:-8958292 Msa0755170:five_prime_UTR
TCTCTGTAACATGAGGTGAC+TGG 0.685080 5_2:-8957099 Msa0755170:five_prime_UTR
TGAATGGAGAAAGTAAGGTG+AGG 0.702390 5_2:-8958317 Msa0755170:five_prime_UTR
ACCGGAGCCTCAACTCTCGG+AGG 0.710836 5_2:-8956395 Msa0755170:CDS
TTATCAATATTTGAACTGAG+GGG 0.710943 5_2:+8956124 None:intergenic
TGCTTGCTCTCTGTAACATG+AGG 0.711732 5_2:-8957106 Msa0755170:five_prime_UTR
AGATACATCAACCAACGTAG+CGG 0.730043 5_2:+8958264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATATCATTGATTTAT+TGG - chr5_2:8956634-8956653 Msa0755170:intron 15.0%
!!! TTTTGATATCATTGATTTAT+TGG - chr5_2:8956634-8956653 Msa0755170:intron 15.0%
!!! ATTCTTTTGCTTTTCATTTT+AGG - chr5_2:8956240-8956259 Msa0755170:exon 20.0%
!! ATTAAAAGCAATATGATCTT+AGG + chr5_2:8956574-8956593 None:intergenic 20.0%
!!! TAAGATCATATTGCTTTTAA+TGG - chr5_2:8956573-8956592 Msa0755170:intron 20.0%
!! TATGATTTGAATGTGATATA+GGG - chr5_2:8956743-8956762 Msa0755170:intron 20.0%
!! ATAGAGAACTTATGAAAATA+AGG + chr5_2:8956876-8956895 None:intergenic 20.0%
!! TAAAGTTACTTATATAACAC+AGG + chr5_2:8956954-8956973 None:intergenic 20.0%
!! TCTCTTTATGAATAAAATCA+TGG + chr5_2:8957333-8957352 None:intergenic 20.0%
!!! AGTTTAAATGTTTTCTTTGA+TGG - chr5_2:8957501-8957520 Msa0755170:intron 20.0%
!! TTTATCAATATTTGAACTGA+GGG + chr5_2:8958167-8958186 None:intergenic 20.0%
!!! ATTCTTTTGCTTTTCATTTT+AGG - chr5_2:8956240-8956259 Msa0755170:exon 20.0%
!! ATTAAAAGCAATATGATCTT+AGG + chr5_2:8956574-8956593 None:intergenic 20.0%
!!! TAAGATCATATTGCTTTTAA+TGG - chr5_2:8956573-8956592 Msa0755170:intron 20.0%
!! TATGATTTGAATGTGATATA+GGG - chr5_2:8956743-8956762 Msa0755170:intron 20.0%
!! ATAGAGAACTTATGAAAATA+AGG + chr5_2:8956876-8956895 None:intergenic 20.0%
!! TAAAGTTACTTATATAACAC+AGG + chr5_2:8956954-8956973 None:intergenic 20.0%
!! TCTCTTTATGAATAAAATCA+TGG + chr5_2:8957333-8957352 None:intergenic 20.0%
!!! AGTTTAAATGTTTTCTTTGA+TGG - chr5_2:8957501-8957520 Msa0755170:intron 20.0%
!! TTTATCAATATTTGAACTGA+GGG + chr5_2:8958167-8958186 None:intergenic 20.0%
!!! AAAATTCGTTTTGAGTTTTC+AGG - chr5_2:8956101-8956120 Msa0755170:three_prime_UTR 25.0%
!!! AGGTGGTAAATTTTGAAAAT+TGG + chr5_2:8956129-8956148 None:intergenic 25.0%
! GAAAGATCAAATCGATAATA+AGG + chr5_2:8956149-8956168 None:intergenic 25.0%
!! CATCGTTTTATCCAATTATT+AGG - chr5_2:8956168-8956187 Msa0755170:three_prime_UTR 25.0%
! GTATGATTTGAATGTGATAT+AGG - chr5_2:8956742-8956761 Msa0755170:intron 25.0%
!! TATTTGCTGACATAATCATT+TGG - chr5_2:8956792-8956811 Msa0755170:intron 25.0%
! GTATTAATTATTGCCCTATT+TGG + chr5_2:8957006-8957025 None:intergenic 25.0%
! ACAATGTAAAACTTTGAACA+TGG - chr5_2:8957024-8957043 Msa0755170:CDS 25.0%
! ATTAAATCAGAAGCAAATAC+TGG + chr5_2:8957114-8957133 None:intergenic 25.0%
! GAAAAAAGTTGACAACTATT+TGG - chr5_2:8957351-8957370 Msa0755170:intron 25.0%
! CATGTGCTTTGTATAATAAT+TGG - chr5_2:8957421-8957440 Msa0755170:intron 25.0%
!!! TAACAGGTAAGCTTTTATTT+TGG - chr5_2:8957669-8957688 Msa0755170:intron 25.0%
! TTATCAATATTTGAACTGAG+GGG + chr5_2:8958166-8958185 None:intergenic 25.0%
! GTTTATCAATATTTGAACTG+AGG + chr5_2:8958168-8958187 None:intergenic 25.0%
!!! AAAATTCGTTTTGAGTTTTC+AGG - chr5_2:8956101-8956120 Msa0755170:three_prime_UTR 25.0%
!!! AGGTGGTAAATTTTGAAAAT+TGG + chr5_2:8956129-8956148 None:intergenic 25.0%
! GAAAGATCAAATCGATAATA+AGG + chr5_2:8956149-8956168 None:intergenic 25.0%
!! CATCGTTTTATCCAATTATT+AGG - chr5_2:8956168-8956187 Msa0755170:three_prime_UTR 25.0%
! GTATGATTTGAATGTGATAT+AGG - chr5_2:8956742-8956761 Msa0755170:intron 25.0%
!! TATTTGCTGACATAATCATT+TGG - chr5_2:8956792-8956811 Msa0755170:intron 25.0%
! GTATTAATTATTGCCCTATT+TGG + chr5_2:8957006-8957025 None:intergenic 25.0%
! ACAATGTAAAACTTTGAACA+TGG - chr5_2:8957024-8957043 Msa0755170:CDS 25.0%
! ATTAAATCAGAAGCAAATAC+TGG + chr5_2:8957114-8957133 None:intergenic 25.0%
! GAAAAAAGTTGACAACTATT+TGG - chr5_2:8957351-8957370 Msa0755170:intron 25.0%
! CATGTGCTTTGTATAATAAT+TGG - chr5_2:8957421-8957440 Msa0755170:intron 25.0%
!!! TAACAGGTAAGCTTTTATTT+TGG - chr5_2:8957669-8957688 Msa0755170:intron 25.0%
! TTATCAATATTTGAACTGAG+GGG + chr5_2:8958166-8958185 None:intergenic 25.0%
! GTTTATCAATATTTGAACTG+AGG + chr5_2:8958168-8958187 None:intergenic 25.0%
!!! AAGTGAAACGTTTTTTGTGA+GGG + chr5_2:8956071-8956090 None:intergenic 30.0%
AGATCAAATCGATAATAAGG+TGG + chr5_2:8956146-8956165 None:intergenic 30.0%
ACATACAGCAACCTAATAAT+TGG + chr5_2:8956182-8956201 None:intergenic 30.0%
TACAAGATAAACGAGATTGA+AGG + chr5_2:8956304-8956323 None:intergenic 30.0%
GAATTGATTGAATCGTGATT+TGG + chr5_2:8956329-8956348 None:intergenic 30.0%
ATATGATCTTAGGCTCTATT+TGG + chr5_2:8956564-8956583 None:intergenic 30.0%
AACAAGTTGCTTATCCAAAT+AGG - chr5_2:8956989-8957008 Msa0755170:intron 30.0%
ACAAGTTGCTTATCCAAATA+GGG - chr5_2:8956990-8957009 Msa0755170:intron 30.0%
AGATGAAGTCAAAACAAAAG+TGG + chr5_2:8957054-8957073 None:intergenic 30.0%
! AGGAATCGTCTTACAAATTT+TGG - chr5_2:8957291-8957310 Msa0755170:intron 30.0%
!! ATCGTCTTACAAATTTTGGT+CGG - chr5_2:8957295-8957314 Msa0755170:intron 30.0%
! AACTTATGTTTTGTGTTGTC+AGG - chr5_2:8957398-8957417 Msa0755170:intron 30.0%
GTGCTTTGTATAATAATTGG+TGG - chr5_2:8957424-8957443 Msa0755170:intron 30.0%
TTTATCTTACCACTAAGCAT+CGG + chr5_2:8957450-8957469 None:intergenic 30.0%
! TTTCTTTGATGGCATTGTTA+CGG - chr5_2:8957512-8957531 Msa0755170:intron 30.0%
! TGTTTTCTGAATCTGATAAC+AGG - chr5_2:8957653-8957672 Msa0755170:intron 30.0%
!! AAGATCTGAATGCTTTAGAA+AGG - chr5_2:8957763-8957782 Msa0755170:intron 30.0%
TAATCACTCATCTCAAGATA+TGG + chr5_2:8957803-8957822 None:intergenic 30.0%
!!! TTGTTTTGTGATTTGCTGAA+TGG - chr5_2:8957836-8957855 Msa0755170:intron 30.0%
TATGAAACAGGAGTTCAATA+AGG - chr5_2:8958055-8958074 Msa0755170:intron 30.0%
TTCAATAAGGTTTGCATCAT+TGG - chr5_2:8958068-8958087 Msa0755170:intron 30.0%
ACGCAAAAGCAAACAATTTA+TGG + chr5_2:8958196-8958215 None:intergenic 30.0%
!!! AAGTGAAACGTTTTTTGTGA+GGG + chr5_2:8956071-8956090 None:intergenic 30.0%
AGATCAAATCGATAATAAGG+TGG + chr5_2:8956146-8956165 None:intergenic 30.0%
ACATACAGCAACCTAATAAT+TGG + chr5_2:8956182-8956201 None:intergenic 30.0%
TACAAGATAAACGAGATTGA+AGG + chr5_2:8956304-8956323 None:intergenic 30.0%
GAATTGATTGAATCGTGATT+TGG + chr5_2:8956329-8956348 None:intergenic 30.0%
ATATGATCTTAGGCTCTATT+TGG + chr5_2:8956564-8956583 None:intergenic 30.0%
AACAAGTTGCTTATCCAAAT+AGG - chr5_2:8956989-8957008 Msa0755170:intron 30.0%
ACAAGTTGCTTATCCAAATA+GGG - chr5_2:8956990-8957009 Msa0755170:intron 30.0%
AGATGAAGTCAAAACAAAAG+TGG + chr5_2:8957054-8957073 None:intergenic 30.0%
! AGGAATCGTCTTACAAATTT+TGG - chr5_2:8957291-8957310 Msa0755170:intron 30.0%
!! ATCGTCTTACAAATTTTGGT+CGG - chr5_2:8957295-8957314 Msa0755170:intron 30.0%
! AACTTATGTTTTGTGTTGTC+AGG - chr5_2:8957398-8957417 Msa0755170:intron 30.0%
GTGCTTTGTATAATAATTGG+TGG - chr5_2:8957424-8957443 Msa0755170:intron 30.0%
TTTATCTTACCACTAAGCAT+CGG + chr5_2:8957450-8957469 None:intergenic 30.0%
! TTTCTTTGATGGCATTGTTA+CGG - chr5_2:8957512-8957531 Msa0755170:intron 30.0%
! TGTTTTCTGAATCTGATAAC+AGG - chr5_2:8957653-8957672 Msa0755170:intron 30.0%
!! AAGATCTGAATGCTTTAGAA+AGG - chr5_2:8957763-8957782 Msa0755170:intron 30.0%
TAATCACTCATCTCAAGATA+TGG + chr5_2:8957803-8957822 None:intergenic 30.0%
!!! TTGTTTTGTGATTTGCTGAA+TGG - chr5_2:8957836-8957855 Msa0755170:intron 30.0%
TATGAAACAGGAGTTCAATA+AGG - chr5_2:8958055-8958074 Msa0755170:intron 30.0%
TTCAATAAGGTTTGCATCAT+TGG - chr5_2:8958068-8958087 Msa0755170:intron 30.0%
ACGCAAAAGCAAACAATTTA+TGG + chr5_2:8958196-8958215 None:intergenic 30.0%
! TTTTTGTGAGGGTTACTTCT+TGG + chr5_2:8956060-8956079 None:intergenic 35.0%
!!! GAAGTGAAACGTTTTTTGTG+AGG + chr5_2:8956072-8956091 None:intergenic 35.0%
ACAAATAAGCCAATCCAAAC+AGG + chr5_2:8956769-8956788 None:intergenic 35.0%
! AATCATTTGGAACACTGTCT+AGG - chr5_2:8956805-8956824 Msa0755170:intron 35.0%
AAACAGTAACGTACATATCC+TGG + chr5_2:8957229-8957248 None:intergenic 35.0%
TACGTTACTGTTTCAACACA+GGG - chr5_2:8957236-8957255 Msa0755170:intron 35.0%
CATTTAAACTTGCAGTGTAC+TGG + chr5_2:8957491-8957510 None:intergenic 35.0%
! TTCTTTGATGGCATTGTTAC+GGG - chr5_2:8957513-8957532 Msa0755170:intron 35.0%
ATGTAATAACCACCAACCAA+GGG + chr5_2:8957548-8957567 None:intergenic 35.0%
AATGTAATAACCACCAACCA+AGG + chr5_2:8957549-8957568 None:intergenic 35.0%
!! TGGTTGGTGGTTATTACATT+CGG - chr5_2:8957549-8957568 Msa0755170:intron 35.0%
! GAAGTAAGATCGTTCAGTTT+TGG - chr5_2:8957701-8957720 Msa0755170:intron 35.0%
!!! TTTTGTGATTTGCTGAATGG+TGG - chr5_2:8957839-8957858 Msa0755170:intron 35.0%
AATTTCGGCAAGTTCTTAAC+CGG - chr5_2:8957874-8957893 Msa0755170:intron 35.0%
CCAGCATGTATTAATATGCT+TGG + chr5_2:8957928-8957947 None:intergenic 35.0%
CCAAGCATATTAATACATGC+TGG - chr5_2:8957925-8957944 Msa0755170:intron 35.0%
TTAATACATGCTGGTGTGAT+CGG - chr5_2:8957934-8957953 Msa0755170:intron 35.0%
GTTACAGTATGCTATGAAAC+AGG - chr5_2:8958043-8958062 Msa0755170:intron 35.0%
AGAAATCAATAGCACGCAAA+AGG + chr5_2:8958112-8958131 None:intergenic 35.0%
! ATGTTTAGAGTGAAGAACAG+AGG + chr5_2:8958258-8958277 None:intergenic 35.0%
! TTTTTGTGAGGGTTACTTCT+TGG + chr5_2:8956060-8956079 None:intergenic 35.0%
!!! GAAGTGAAACGTTTTTTGTG+AGG + chr5_2:8956072-8956091 None:intergenic 35.0%
ACAAATAAGCCAATCCAAAC+AGG + chr5_2:8956769-8956788 None:intergenic 35.0%
! AATCATTTGGAACACTGTCT+AGG - chr5_2:8956805-8956824 Msa0755170:intron 35.0%
AAACAGTAACGTACATATCC+TGG + chr5_2:8957229-8957248 None:intergenic 35.0%
TACGTTACTGTTTCAACACA+GGG - chr5_2:8957236-8957255 Msa0755170:intron 35.0%
CATTTAAACTTGCAGTGTAC+TGG + chr5_2:8957491-8957510 None:intergenic 35.0%
! TTCTTTGATGGCATTGTTAC+GGG - chr5_2:8957513-8957532 Msa0755170:intron 35.0%
ATGTAATAACCACCAACCAA+GGG + chr5_2:8957548-8957567 None:intergenic 35.0%
AATGTAATAACCACCAACCA+AGG + chr5_2:8957549-8957568 None:intergenic 35.0%
!! TGGTTGGTGGTTATTACATT+CGG - chr5_2:8957549-8957568 Msa0755170:intron 35.0%
! GAAGTAAGATCGTTCAGTTT+TGG - chr5_2:8957701-8957720 Msa0755170:intron 35.0%
!!! TTTTGTGATTTGCTGAATGG+TGG - chr5_2:8957839-8957858 Msa0755170:intron 35.0%
AATTTCGGCAAGTTCTTAAC+CGG - chr5_2:8957874-8957893 Msa0755170:intron 35.0%
CCAGCATGTATTAATATGCT+TGG + chr5_2:8957928-8957947 None:intergenic 35.0%
CCAAGCATATTAATACATGC+TGG - chr5_2:8957925-8957944 Msa0755170:intron 35.0%
TTAATACATGCTGGTGTGAT+CGG - chr5_2:8957934-8957953 Msa0755170:intron 35.0%
GTTACAGTATGCTATGAAAC+AGG - chr5_2:8958043-8958062 Msa0755170:intron 35.0%
AGAAATCAATAGCACGCAAA+AGG + chr5_2:8958112-8958131 None:intergenic 35.0%
! ATGTTTAGAGTGAAGAACAG+AGG + chr5_2:8958258-8958277 None:intergenic 35.0%
TGAATGGAGAAAGTAAGGTG+AGG - chr5_2:8955970-8955989 Msa0755170:three_prime_UTR 40.0%
AGATACATCAACCAACGTAG+CGG + chr5_2:8956026-8956045 None:intergenic 40.0%
TGATTCACGACGATACAACA+TGG - chr5_2:8956599-8956618 Msa0755170:intron 40.0%
GTTCGTGATCTTAAGCTACT+AGG - chr5_2:8956668-8956687 Msa0755170:CDS 40.0%
! AGCTACTAGGTTTGCTTTCT+TGG - chr5_2:8956681-8956700 Msa0755170:CDS 40.0%
AATGTGATATAGGGCCTGTT+TGG - chr5_2:8956752-8956771 Msa0755170:intron 40.0%
TCGTAAGCACTAAGCTATGA+GGG + chr5_2:8957138-8957157 None:intergenic 40.0%
GTACGTTACTGTTTCAACAC+AGG - chr5_2:8957235-8957254 Msa0755170:intron 40.0%
!! GCTTCTCTTTTTGGCACTTT+TGG - chr5_2:8957261-8957280 Msa0755170:intron 40.0%
!! TTTTTGGCACTTTTGGTCTC+AGG - chr5_2:8957268-8957287 Msa0755170:intron 40.0%
! TCTTTGATGGCATTGTTACG+GGG - chr5_2:8957514-8957533 Msa0755170:intron 40.0%
TGTAATAACCACCAACCAAG+GGG + chr5_2:8957547-8957566 None:intergenic 40.0%
AGAAGAGAGTTCCAATGCAA+AGG + chr5_2:8957968-8957987 None:intergenic 40.0%
TAGCCATATTGTGTTTCCTG+TGG - chr5_2:8958004-8958023 Msa0755170:intron 40.0%
CATCCACAGGAAACACAATA+TGG + chr5_2:8958010-8958029 None:intergenic 40.0%
TATCTTCATCACTCATCCAC+AGG + chr5_2:8958023-8958042 None:intergenic 40.0%
TCAATATTTGAACTGAGGGG+AGG + chr5_2:8958163-8958182 None:intergenic 40.0%
! TTTAGAGTGAAGAACAGAGG+TGG + chr5_2:8958255-8958274 None:intergenic 40.0%
CTCTGTAGAAACACAGCTAT+TGG - chr5_2:8958278-8958297 Msa0755170:five_prime_UTR 40.0%
ATTGAGCAGCACTCTTGTAA+TGG + chr5_2:8958307-8958326 None:intergenic 40.0%
TGAATGGAGAAAGTAAGGTG+AGG - chr5_2:8955970-8955989 Msa0755170:three_prime_UTR 40.0%
AGATACATCAACCAACGTAG+CGG + chr5_2:8956026-8956045 None:intergenic 40.0%
TGATTCACGACGATACAACA+TGG - chr5_2:8956599-8956618 Msa0755170:intron 40.0%
GTTCGTGATCTTAAGCTACT+AGG - chr5_2:8956668-8956687 Msa0755170:CDS 40.0%
! AGCTACTAGGTTTGCTTTCT+TGG - chr5_2:8956681-8956700 Msa0755170:CDS 40.0%
AATGTGATATAGGGCCTGTT+TGG - chr5_2:8956752-8956771 Msa0755170:intron 40.0%
TCGTAAGCACTAAGCTATGA+GGG + chr5_2:8957138-8957157 None:intergenic 40.0%
GTACGTTACTGTTTCAACAC+AGG - chr5_2:8957235-8957254 Msa0755170:intron 40.0%
!! GCTTCTCTTTTTGGCACTTT+TGG - chr5_2:8957261-8957280 Msa0755170:intron 40.0%
!! TTTTTGGCACTTTTGGTCTC+AGG - chr5_2:8957268-8957287 Msa0755170:intron 40.0%
! TCTTTGATGGCATTGTTACG+GGG - chr5_2:8957514-8957533 Msa0755170:intron 40.0%
TGTAATAACCACCAACCAAG+GGG + chr5_2:8957547-8957566 None:intergenic 40.0%
AGAAGAGAGTTCCAATGCAA+AGG + chr5_2:8957968-8957987 None:intergenic 40.0%
TAGCCATATTGTGTTTCCTG+TGG - chr5_2:8958004-8958023 Msa0755170:intron 40.0%
CATCCACAGGAAACACAATA+TGG + chr5_2:8958010-8958029 None:intergenic 40.0%
TATCTTCATCACTCATCCAC+AGG + chr5_2:8958023-8958042 None:intergenic 40.0%
TCAATATTTGAACTGAGGGG+AGG + chr5_2:8958163-8958182 None:intergenic 40.0%
! TTTAGAGTGAAGAACAGAGG+TGG + chr5_2:8958255-8958274 None:intergenic 40.0%
CTCTGTAGAAACACAGCTAT+TGG - chr5_2:8958278-8958297 Msa0755170:five_prime_UTR 40.0%
ATTGAGCAGCACTCTTGTAA+TGG + chr5_2:8958307-8958326 None:intergenic 40.0%
! AGGACAGAGTATCCTTCCAA+AGG - chr5_2:8955995-8956014 Msa0755170:three_prime_UTR 45.0%
! CAGAGTATCCTTCCAAAGGT+TGG - chr5_2:8955999-8956018 Msa0755170:three_prime_UTR 45.0%
CTCACGAGTGATTATGTGAG+TGG + chr5_2:8956359-8956378 None:intergenic 45.0%
! TGCTTTCTTGGTGCATAGAC+AGG - chr5_2:8956693-8956712 Msa0755170:CDS 45.0%
GATATAGGGCCTGTTTGGAT+TGG - chr5_2:8956757-8956776 Msa0755170:intron 45.0%
CTCGTAAGCACTAAGCTATG+AGG + chr5_2:8957139-8957158 None:intergenic 45.0%
TGCTTGCTCTCTGTAACATG+AGG - chr5_2:8957181-8957200 Msa0755170:intron 45.0%
TCTCTGTAACATGAGGTGAC+TGG - chr5_2:8957188-8957207 Msa0755170:intron 45.0%
AACATGAGGTGACTGGAAGA+TGG - chr5_2:8957195-8957214 Msa0755170:intron 45.0%
TGGAAGATGGATGACATACC+AGG - chr5_2:8957208-8957227 Msa0755170:intron 45.0%
! CACAGGGAAGCTTCTCTTTT+TGG - chr5_2:8957252-8957271 Msa0755170:intron 45.0%
ATATTTGAACTGAGGGGAGG+AGG + chr5_2:8958160-8958179 None:intergenic 45.0%
AGTGAAGAACAGAGGTGGAA+AGG + chr5_2:8958250-8958269 None:intergenic 45.0%
! AGGACAGAGTATCCTTCCAA+AGG - chr5_2:8955995-8956014 Msa0755170:three_prime_UTR 45.0%
! CAGAGTATCCTTCCAAAGGT+TGG - chr5_2:8955999-8956018 Msa0755170:three_prime_UTR 45.0%
CTCACGAGTGATTATGTGAG+TGG + chr5_2:8956359-8956378 None:intergenic 45.0%
! TGCTTTCTTGGTGCATAGAC+AGG - chr5_2:8956693-8956712 Msa0755170:CDS 45.0%
GATATAGGGCCTGTTTGGAT+TGG - chr5_2:8956757-8956776 Msa0755170:intron 45.0%
CTCGTAAGCACTAAGCTATG+AGG + chr5_2:8957139-8957158 None:intergenic 45.0%
TGCTTGCTCTCTGTAACATG+AGG - chr5_2:8957181-8957200 Msa0755170:intron 45.0%
TCTCTGTAACATGAGGTGAC+TGG - chr5_2:8957188-8957207 Msa0755170:intron 45.0%
AACATGAGGTGACTGGAAGA+TGG - chr5_2:8957195-8957214 Msa0755170:intron 45.0%
TGGAAGATGGATGACATACC+AGG - chr5_2:8957208-8957227 Msa0755170:intron 45.0%
! CACAGGGAAGCTTCTCTTTT+TGG - chr5_2:8957252-8957271 Msa0755170:intron 45.0%
ATATTTGAACTGAGGGGAGG+AGG + chr5_2:8958160-8958179 None:intergenic 45.0%
AGTGAAGAACAGAGGTGGAA+AGG + chr5_2:8958250-8958269 None:intergenic 45.0%
GGAGAAAGTAAGGTGAGGTG+AGG - chr5_2:8955975-8955994 Msa0755170:three_prime_UTR 50.0%
! TTGGCACTTTTGGTCTCAGG+AGG - chr5_2:8957271-8957290 Msa0755170:intron 50.0%
! AATTGGTGGCCGATGCTTAG+TGG - chr5_2:8957438-8957457 Msa0755170:intron 50.0%
TTACGGGGAGAAAATCCCCT+TGG - chr5_2:8957529-8957548 Msa0755170:intron 50.0%
GAGAAAATCCCCTTGGTTGG+TGG - chr5_2:8957536-8957555 Msa0755170:intron 50.0%
GCAAAGAACAGCAACGGCAT+TGG + chr5_2:8957613-8957632 None:intergenic 50.0%
! ATGCCGTTGCTGTTCTTTGC+TGG - chr5_2:8957613-8957632 Msa0755170:intron 50.0%
! TGCCGTTGCTGTTCTTTGCT+GGG - chr5_2:8957614-8957633 Msa0755170:intron 50.0%
GACCCAGCAAAGAACAGCAA+CGG + chr5_2:8957619-8957638 None:intergenic 50.0%
CTGTTCTTTGCTGGGTCTGA+TGG - chr5_2:8957622-8957641 Msa0755170:intron 50.0%
TGGTGCAGCTGGGTGAATTT+CGG - chr5_2:8957859-8957878 Msa0755170:intron 50.0%
TTAACCGGAGCCTCAACTCT+CGG - chr5_2:8957889-8957908 Msa0755170:intron 50.0%
TACATGCTGGTGTGATCGGT+TGG - chr5_2:8957938-8957957 Msa0755170:intron 50.0%
ACATGCTGGTGTGATCGGTT+GGG - chr5_2:8957939-8957958 Msa0755170:intron 50.0%
GAAGAACAGAGGTGGAAAGG+TGG + chr5_2:8958247-8958266 None:intergenic 50.0%
GGAGAAAGTAAGGTGAGGTG+AGG - chr5_2:8955975-8955994 Msa0755170:three_prime_UTR 50.0%
! TTGGCACTTTTGGTCTCAGG+AGG - chr5_2:8957271-8957290 Msa0755170:intron 50.0%
! AATTGGTGGCCGATGCTTAG+TGG - chr5_2:8957438-8957457 Msa0755170:intron 50.0%
TTACGGGGAGAAAATCCCCT+TGG - chr5_2:8957529-8957548 Msa0755170:intron 50.0%
GAGAAAATCCCCTTGGTTGG+TGG - chr5_2:8957536-8957555 Msa0755170:intron 50.0%
GCAAAGAACAGCAACGGCAT+TGG + chr5_2:8957613-8957632 None:intergenic 50.0%
! ATGCCGTTGCTGTTCTTTGC+TGG - chr5_2:8957613-8957632 Msa0755170:intron 50.0%
! TGCCGTTGCTGTTCTTTGCT+GGG - chr5_2:8957614-8957633 Msa0755170:intron 50.0%
GACCCAGCAAAGAACAGCAA+CGG + chr5_2:8957619-8957638 None:intergenic 50.0%
CTGTTCTTTGCTGGGTCTGA+TGG - chr5_2:8957622-8957641 Msa0755170:intron 50.0%
TGGTGCAGCTGGGTGAATTT+CGG - chr5_2:8957859-8957878 Msa0755170:intron 50.0%
TTAACCGGAGCCTCAACTCT+CGG - chr5_2:8957889-8957908 Msa0755170:intron 50.0%
TACATGCTGGTGTGATCGGT+TGG - chr5_2:8957938-8957957 Msa0755170:intron 50.0%
ACATGCTGGTGTGATCGGTT+GGG - chr5_2:8957939-8957958 Msa0755170:intron 50.0%
GAAGAACAGAGGTGGAAAGG+TGG + chr5_2:8958247-8958266 None:intergenic 50.0%
GTAGCGGTCCAACCTTTGGA+AGG + chr5_2:8956010-8956029 None:intergenic 55.0%
CAACGTAGCGGTCCAACCTT+TGG + chr5_2:8956014-8956033 None:intergenic 55.0%
CAAAGGTTGGACCGCTACGT+TGG - chr5_2:8956012-8956031 Msa0755170:three_prime_UTR 55.0%
GGGGAGAAAATCCCCTTGGT+TGG - chr5_2:8957533-8957552 Msa0755170:intron 55.0%
! TTGCTGAATGGTGGTGCAGC+TGG - chr5_2:8957848-8957867 Msa0755170:intron 55.0%
! TGCTGAATGGTGGTGCAGCT+GGG - chr5_2:8957849-8957868 Msa0755170:intron 55.0%
GTGGAAAGGTGGAGAAGGAG+AGG + chr5_2:8958236-8958255 None:intergenic 55.0%
CAGAGGTGGAAAGGTGGAGA+AGG + chr5_2:8958241-8958260 None:intergenic 55.0%
GTAGCGGTCCAACCTTTGGA+AGG + chr5_2:8956010-8956029 None:intergenic 55.0%
CAACGTAGCGGTCCAACCTT+TGG + chr5_2:8956014-8956033 None:intergenic 55.0%
CAAAGGTTGGACCGCTACGT+TGG - chr5_2:8956012-8956031 Msa0755170:three_prime_UTR 55.0%
GGGGAGAAAATCCCCTTGGT+TGG - chr5_2:8957533-8957552 Msa0755170:intron 55.0%
! TTGCTGAATGGTGGTGCAGC+TGG - chr5_2:8957848-8957867 Msa0755170:intron 55.0%
! TGCTGAATGGTGGTGCAGCT+GGG - chr5_2:8957849-8957868 Msa0755170:intron 55.0%
GTGGAAAGGTGGAGAAGGAG+AGG + chr5_2:8958236-8958255 None:intergenic 55.0%
CAGAGGTGGAAAGGTGGAGA+AGG + chr5_2:8958241-8958260 None:intergenic 55.0%
CGCAATGCCTCCGAGAGTTG+AGG + chr5_2:8957902-8957921 None:intergenic 60.0%
CGGTTGGGCAGCCTTTGCAT+TGG - chr5_2:8957954-8957973 Msa0755170:intron 60.0%
CGCAATGCCTCCGAGAGTTG+AGG + chr5_2:8957902-8957921 None:intergenic 60.0%
CGGTTGGGCAGCCTTTGCAT+TGG - chr5_2:8957954-8957973 Msa0755170:intron 60.0%
ACCGGAGCCTCAACTCTCGG+AGG - chr5_2:8957892-8957911 Msa0755170:intron 65.0%
GCCTCCGAGAGTTGAGGCTC+CGG + chr5_2:8957896-8957915 None:intergenic 65.0%
ACCGGAGCCTCAACTCTCGG+AGG - chr5_2:8957892-8957911 Msa0755170:intron 65.0%
GCCTCCGAGAGTTGAGGCTC+CGG + chr5_2:8957896-8957915 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 8955970 8958339 8955970 ID=Msa0755170;Name=Msa0755170
chr5_2 mRNA 8955970 8958339 8955970 ID=Msa0755170-mRNA-1;Parent=Msa0755170;Name=Msa0755170-mRNA-1;_AED=0.02;_eAED=0.02;_QI=197|1|1|1|0.75|0.6|5|280|138
chr5_2 exon 8955970 8956442 8955970 ID=Msa0755170-mRNA-1:exon:4065;Parent=Msa0755170-mRNA-1
chr5_2 exon 8956635 8956719 8956635 ID=Msa0755170-mRNA-1:exon:4064;Parent=Msa0755170-mRNA-1
chr5_2 exon 8956850 8956889 8956850 ID=Msa0755170-mRNA-1:exon:4063;Parent=Msa0755170-mRNA-1
chr5_2 exon 8956997 8957139 8956997 ID=Msa0755170-mRNA-1:exon:4062;Parent=Msa0755170-mRNA-1
chr5_2 exon 8958187 8958339 8958187 ID=Msa0755170-mRNA-1:exon:4061;Parent=Msa0755170-mRNA-1
chr5_2 five_prime_UTR 8958187 8958339 8958187 ID=Msa0755170-mRNA-1:five_prime_utr;Parent=Msa0755170-mRNA-1
chr5_2 five_prime_UTR 8957096 8957139 8957096 ID=Msa0755170-mRNA-1:five_prime_utr;Parent=Msa0755170-mRNA-1
chr5_2 CDS 8956997 8957095 8956997 ID=Msa0755170-mRNA-1:cds;Parent=Msa0755170-mRNA-1
chr5_2 CDS 8956850 8956889 8956850 ID=Msa0755170-mRNA-1:cds;Parent=Msa0755170-mRNA-1
chr5_2 CDS 8956635 8956719 8956635 ID=Msa0755170-mRNA-1:cds;Parent=Msa0755170-mRNA-1
chr5_2 CDS 8956250 8956442 8956250 ID=Msa0755170-mRNA-1:cds;Parent=Msa0755170-mRNA-1
chr5_2 three_prime_UTR 8955970 8956249 8955970 ID=Msa0755170-mRNA-1:three_prime_utr;Parent=Msa0755170-mRNA-1
Gene Sequence

>Msa0755170

ATGGATGACATACCAGGATATGTACGTTACTGTTTCAACACAGGGAAGCTTCTCTTTTTGGCACTTTTGGTCTCAGGAGGAATCGTCTTACAAATTTTGGCATGTGCTTTGTATAATAATTGGTGGCCGATGCTTAGTGCTATAACGTATGTGCTTCTTCCAATGCCGTTGCTGTTCTTTGCTGGGTCTGATGGTTCTTCTATGTTTTCTGAATCTGATAACAGCTGGGTGAATTTCGGCAAGTTCTTAACCGGAGCCTCAACTCTCGGAGGCATTGCGATTCCAAGCATATTAATACATGCTGGTGTGATCGGTTGGGCAGCCTTTGCATTGGAACTCTCTTCTTTCTTTGTGTTTATATTAGCCATATTGTGTTTCCTGTGGATGAGTGATGAAGATAGTTACAGTATGCTATGA
Protein sequence

>Msa0755170

MDDIPGYVRYCFNTGKLLFLALLVSGGIVLQILACALYNNWWPMLSAITYVLLPMPLLFFAGSDGSSMFSESDNSWVNFGKFLTGASTLGGIAIPSILIHAGVIGWAAFALELSSFFVFILAILCFLWMSDEDSYSML*