AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0762310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0762310 MtrunA17_Chr5g0412591 94.373 622 5 1 1 622 1 592 0.0 1190
Msa0762310 MtrunA17_Chr8g0367891 71.981 621 132 9 1 620 1 580 0.0 838
Msa0762310 MtrunA17_Chr4g0076521 48.467 522 223 7 1 521 1 477 8.50e-162 478
Msa0762310 MtrunA17_Chr5g0401341 46.346 561 251 9 1 552 1 520 1.07e-158 470
Msa0762310 MtrunA17_Chr1g0150191 47.228 523 235 10 1 518 1 487 8.10e-152 452
Msa0762310 MtrunA17_Chr1g0207461 42.989 542 269 7 1 537 1 507 5.69e-146 437
Msa0762310 MtrunA17_Chr2g0290741 40.758 633 284 12 1 610 1 565 4.99e-145 433
Msa0762310 MtrunA17_Chr4g0050021 39.560 546 280 8 5 523 23 545 8.70e-126 387
Msa0762310 MtrunA17_Chr2g0279481 39.964 553 275 9 5 523 23 552 6.27e-125 385
Msa0762310 MtrunA17_Chr2g0303541 37.294 606 321 13 1 590 1 563 6.90e-117 363
Msa0762310 MtrunA17_Chr2g0285511 40.226 532 279 9 1 523 1 502 2.47e-113 353
Msa0762310 MtrunA17_Chr1g0181181 38.241 523 283 7 1 523 1 483 2.01e-110 343
Msa0762310 MtrunA17_Chr8g0382231 35.120 541 277 6 16 536 42 528 2.42e-109 343
Msa0762310 MtrunA17_Chr5g0401451 37.313 536 240 10 23 529 37 505 6.11e-107 337
Msa0762310 MtrunA17_Chr8g0374011 38.031 518 266 9 28 517 22 512 1.14e-104 332
Msa0762310 MtrunA17_Chr2g0320691 35.579 579 327 10 24 591 21 564 1.51e-104 330
Msa0762310 MtrunA17_Chr7g0245201 38.124 501 267 9 22 519 57 517 1.26e-100 320
Msa0762310 MtrunA17_Chr1g0199631 39.228 492 250 11 44 523 8 462 5.21e-100 317
Msa0762310 MtrunA17_Chr4g0010091 36.283 565 293 10 22 581 30 532 1.91e-97 311
Msa0762310 MtrunA17_Chr4g0053841 36.217 497 276 7 23 519 10 465 2.37e-92 297
Msa0762310 MtrunA17_Chr1g0195911 37.288 531 271 14 12 525 18 503 3.82e-92 298
Msa0762310 MtrunA17_Chr7g0268781 36.054 588 313 14 12 584 18 557 1.83e-91 296
Msa0762310 MtrunA17_Chr3g0108331 34.449 508 274 8 21 526 19 469 5.12e-86 280
Msa0762310 MtrunA17_Chr5g0432861 33.861 505 282 8 22 524 21 475 1.12e-76 256
Msa0762310 MtrunA17_Chr1g0191711 30.754 517 276 11 27 535 59 501 1.93e-71 241
Msa0762310 MtrunA17_Chr4g0003891 38.188 309 150 4 211 519 46 313 2.05e-65 222
Msa0762310 MtrunA17_Chr2g0332281 39.159 309 149 4 211 519 24 293 2.06e-62 213
Msa0762310 MtrunA17_Chr4g0023801 29.295 454 250 13 74 519 52 442 2.04e-47 175
Msa0762310 MtrunA17_Chr4g0037661 27.672 524 295 13 27 523 4 470 7.07e-47 174
Msa0762310 MtrunA17_Chr1g0163311 25.591 508 286 12 64 541 36 481 1.26e-41 158
Msa0762310 MtrunA17_Chr1g0206621 37.086 151 94 1 33 183 75 224 1.64e-22 97.1
Msa0762310 MtrunA17_Chr2g0280541 25.060 419 246 10 94 505 1 358 2.39e-21 97.4
Msa0762310 MtrunA17_Chr4g0041491 62.712 59 22 0 1 59 1 59 1.60e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0762310 AT1G67900.5 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT1G67900.1 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT1G67900.3 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT1G67900.6 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT1G67900.2 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT1G67900.4 73.291 629 144 11 1 620 1 614 0.0 844
Msa0762310 AT3G26490.1 49.615 649 235 16 1 628 1 578 0.0 552
Msa0762310 AT3G26490.3 49.615 649 235 16 1 628 1 578 0.0 552
Msa0762310 AT3G26490.4 49.615 649 235 16 1 628 1 578 0.0 552
Msa0762310 AT3G26490.2 53.265 490 175 13 1 469 1 457 2.19e-160 470
Msa0762310 AT2G14820.2 45.149 536 247 8 1 528 1 497 3.82e-151 451
Msa0762310 AT2G14820.1 45.149 536 247 8 1 528 1 497 3.82e-151 451
Msa0762310 AT5G47800.3 43.152 533 257 7 1 530 1 490 8.96e-147 437
Msa0762310 AT5G47800.1 43.152 533 257 7 1 530 1 490 8.96e-147 437
Msa0762310 AT5G47800.4 43.152 533 257 7 1 530 1 490 8.96e-147 437
Msa0762310 AT5G47800.2 43.152 533 257 7 1 530 114 603 6.83e-145 437
Msa0762310 AT5G67440.3 43.619 525 247 8 1 522 1 479 3.00e-142 427
Msa0762310 AT5G67440.1 43.619 525 247 8 1 522 1 479 3.00e-142 427
Msa0762310 AT4G37590.1 40.336 595 263 12 1 582 1 516 2.03e-140 422
Msa0762310 AT4G31820.1 42.722 529 240 10 1 523 1 472 2.41e-132 401
Msa0762310 AT5G67440.2 43.595 484 225 7 41 522 5 442 2.21e-127 387
Msa0762310 AT2G23050.1 41.588 529 228 11 1 523 1 454 7.42e-127 384
Msa0762310 AT5G64330.2 36.498 611 314 9 7 549 33 637 1.40e-120 374
Msa0762310 AT5G64330.1 35.913 646 332 12 7 584 33 664 2.65e-120 375
Msa0762310 AT4G31820.2 42.017 476 214 9 53 523 1 419 1.20e-115 356
Msa0762310 AT5G64330.3 37.133 579 290 9 39 549 6 578 3.60e-115 358
Msa0762310 AT1G30440.1 39.171 531 296 9 1 523 1 512 5.46e-113 354
Msa0762310 AT5G03250.1 37.782 532 296 6 1 523 1 506 6.41e-113 351
Msa0762310 AT5G13600.1 40.189 530 277 10 1 523 1 497 1.05e-109 343
Msa0762310 AT5G67385.1 38.976 508 258 8 15 519 16 474 1.91e-104 330
Msa0762310 AT5G48800.1 39.053 507 249 9 22 523 37 488 3.23e-98 314
Msa0762310 AT5G67385.4 38.025 476 245 7 47 519 1 429 3.03e-95 304
Msa0762310 AT5G67385.5 38.025 476 245 7 47 519 1 429 3.03e-95 304
Msa0762310 AT5G67385.2 38.025 476 245 7 47 519 1 429 3.03e-95 304
Msa0762310 AT5G67385.3 38.025 476 245 7 47 519 1 429 3.03e-95 304
Msa0762310 AT5G10250.2 34.357 521 259 8 22 519 46 506 2.20e-91 296
Msa0762310 AT5G66560.1 36.029 544 258 10 28 517 21 528 8.67e-91 296
Msa0762310 AT3G08570.1 37.255 510 276 11 13 519 22 490 1.00e-90 294
Msa0762310 AT3G44820.1 33.786 589 330 15 26 591 23 574 1.22e-90 295
Msa0762310 AT1G03010.2 37.972 503 272 12 24 519 7 476 1.28e-89 291
Msa0762310 AT1G03010.1 37.972 503 272 12 24 519 34 503 2.65e-89 291
Msa0762310 AT1G03010.3 37.597 516 280 13 11 519 22 502 3.33e-89 291
Msa0762310 AT5G66560.3 36.023 533 251 10 39 517 3 499 4.01e-89 291
Msa0762310 AT5G66560.4 38.696 460 234 9 70 517 66 489 9.01e-89 289
Msa0762310 AT5G10250.1 34.442 511 257 9 22 519 46 491 3.88e-88 287
Msa0762310 AT2G23050.2 40.436 413 169 8 113 523 1 338 1.17e-86 276
Msa0762310 AT3G49970.1 36.344 465 216 11 39 502 7 392 5.17e-85 276
Msa0762310 AT3G49970.2 36.344 465 216 11 39 502 7 392 5.17e-85 276
Msa0762310 AT3G08660.1 35.000 520 256 12 8 519 21 466 1.59e-82 271
Msa0762310 AT2G47860.1 34.990 503 277 10 24 519 35 494 2.22e-80 267
Msa0762310 AT2G30520.1 32.604 503 285 9 21 523 25 473 1.81e-78 261
Msa0762310 AT3G50840.1 34.921 504 276 8 28 529 18 471 3.02e-77 257
Msa0762310 AT5G03250.2 35.374 441 250 6 113 544 1 415 7.82e-76 251
Msa0762310 AT2G47860.3 33.460 526 277 11 24 519 35 517 3.08e-75 254
Msa0762310 AT3G08660.3 35.076 459 224 10 69 519 14 406 9.55e-73 244
Msa0762310 AT2G30520.2 32.239 518 289 13 46 563 2 457 2.43e-72 243
Msa0762310 AT2G30520.3 32.239 518 289 13 46 563 2 457 2.43e-72 243
Msa0762310 AT3G50840.2 34.021 485 268 8 47 529 1 435 1.04e-70 239
Msa0762310 AT3G50840.3 34.021 485 268 8 47 529 1 435 1.04e-70 239
Msa0762310 AT5G66560.2 33.131 495 241 10 28 468 21 479 1.82e-64 221
Msa0762310 AT1G52770.1 39.677 310 152 4 211 519 28 303 9.87e-64 218
Msa0762310 AT3G08660.2 31.770 469 242 12 8 470 21 417 2.51e-60 208
Msa0762310 AT3G15570.1 37.705 305 151 4 220 519 38 308 6.57e-59 205
Msa0762310 AT2G47860.4 32.662 447 261 10 24 468 35 443 4.36e-57 200
Msa0762310 AT2G47860.5 33.092 414 232 9 113 519 1 376 5.85e-54 193
Msa0762310 AT2G47860.2 33.092 414 232 9 113 519 1 376 5.85e-54 193
Msa0762310 AT2G47860.6 31.064 470 261 11 24 468 35 466 3.23e-52 187
Msa0762310 AT3G19850.1 25.847 472 268 7 69 517 51 463 3.50e-49 181
Msa0762310 AT3G03510.1 30.000 450 207 12 82 519 8 361 1.76e-41 157
Msa0762310 AT5G48130.1 25.828 453 270 9 70 517 76 467 1.78e-41 160
Msa0762310 AT1G50280.2 24.569 464 276 8 70 513 18 427 5.66e-40 153
Msa0762310 AT1G50280.1 24.569 464 276 8 70 513 51 460 7.06e-40 154
Msa0762310 AT5G17580.2 27.376 526 312 11 8 526 2 464 5.26e-38 149
Msa0762310 AT5G17580.1 27.539 512 301 11 22 526 1 449 1.68e-37 147
Msa0762310 AT3G22104.1 25.773 485 278 13 28 502 6 418 1.45e-32 132
Msa0762310 AT3G22104.4 25.182 413 230 10 98 502 2 343 3.41e-23 103
Msa0762310 AT3G22104.2 25.182 413 230 10 98 502 2 343 3.41e-23 103
Msa0762310 AT3G22104.3 25.182 413 230 10 98 502 2 343 3.41e-23 103

Find 145 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTAGCATAGACCTTTAT+TGG 0.142674 5_2:+20332960 Msa0762310:CDS
GAGGATAATTGGAGTGTTTC+TGG 0.144528 5_2:+20335069 Msa0762310:CDS
CTTCTTTCTATTTCCGTTCT+TGG 0.164934 5_2:-20333080 None:intergenic
CTTTCACTCTTGTTGAGTTC+TGG 0.171118 5_2:-20334796 None:intergenic
CTCAAAATTATTATTATTAT+AGG 0.177140 5_2:-20335449 None:intergenic
TTTCCGTTCTTGGAAATATT+TGG 0.180699 5_2:-20333070 None:intergenic
AATCAGGAAGTTGGACTATT+TGG 0.186197 5_2:-20332511 None:intergenic
CATGATGAATTTGATGAAAA+TGG 0.208213 5_2:+20335147 Msa0762310:CDS
ACTTGAACAACAACTCTTAA+AGG 0.232478 5_2:-20334898 None:intergenic
AAGCAAAGATAGAATTTCTT+TGG 0.270418 5_2:-20331607 None:intergenic
AAGAAAAGTTCAGAATTCTT+AGG 0.276907 5_2:-20331650 None:intergenic
TCCAAGTTTCATAAACTTCA+TGG 0.285144 5_2:-20331898 None:intergenic
AGAGTTGAAGACTTTGATTT+TGG 0.291628 5_2:-20335102 None:intergenic
TTTCAACTTCTTCAGTCATT+TGG 0.299710 5_2:-20332643 None:intergenic
TGATCATATCTTGCAAATTC+TGG 0.304119 5_2:-20333655 None:intergenic
GTATTCAAGGTGAGGATAAT+TGG 0.310231 5_2:+20335058 Msa0762310:CDS
AGCTTGCTTCCAACAGAAAA+AGG 0.312871 5_2:+20333190 Msa0762310:CDS
TGAAGAAGCATTGAGAATAT+TGG 0.313880 5_2:-20333254 None:intergenic
GTAGACAAATACAGCAATAT+TGG 0.314454 5_2:-20335558 None:intergenic
TTCAAAGCATAGGCTTCTAT+TGG 0.325252 5_2:+20333156 Msa0762310:CDS
CCACAAAAGCGTTGCATTTG+TGG 0.325463 5_2:+20332750 Msa0762310:CDS
ATGACTGAAGAAGTTGAAAA+AGG 0.332313 5_2:+20332647 Msa0762310:CDS
CTATCATTGTTCTCCAATAA+AGG 0.335482 5_2:-20332973 None:intergenic
GATATCGGAATAGGAATCTT+TGG 0.335729 5_2:+20335514 Msa0762310:three_prime_UTR
TGAAAATTTATGCATCTAGA+TGG 0.337434 5_2:+20333044 Msa0762310:CDS
TCTACACTTCGGAAGCTATA+AGG 0.341634 5_2:+20331937 Msa0762310:CDS
GCCATGAAGTTTATGAAACT+TGG 0.345527 5_2:+20331897 Msa0762310:exon
TCCGAAGTGTAGAAAGTATC+TGG 0.347377 5_2:-20331927 None:intergenic
TAAAATGTCAAAATCACTTA+AGG 0.350973 5_2:+20331407 Msa0762310:five_prime_UTR
GTTAGGATAGATGACATTAA+TGG 0.357070 5_2:-20331493 None:intergenic
TCCACTCCACCCGGAAAATC+AGG 0.369267 5_2:-20332527 None:intergenic
TGTGACAAACTCACCTTTGT+AGG 0.375606 5_2:-20332836 None:intergenic
AAAATAAATATTCTTCATCT+TGG 0.386333 5_2:+20331745 Msa0762310:five_prime_UTR
TCTTTGCAGGCATAAGCATT+TGG 0.386345 5_2:-20332453 None:intergenic
TGAAAGAAGGCGATCACGTT+CGG 0.388598 5_2:+20333471 Msa0762310:CDS
TTGGTAAATGATGATGGAAT+TGG 0.390964 5_2:+20335171 Msa0762310:CDS
ATTCTGAGCATAATCTTTGC+AGG 0.392741 5_2:-20332466 None:intergenic
TGAAAAGTTGATATCGGAAT+AGG 0.402848 5_2:+20335505 Msa0762310:three_prime_UTR
TGTTTGTAAGGGTAATTTAG+AGG 0.403451 5_2:+20335325 Msa0762310:CDS
GCATTTGTGGTCGGAAGACC+TGG 0.409040 5_2:+20332763 Msa0762310:CDS
TGAGTTGGTGATGACTATAT+TGG 0.420160 5_2:+20333387 Msa0762310:CDS
GTTATAACTATTTGACTGAA+TGG 0.421950 5_2:+20331429 Msa0762310:five_prime_UTR
TTTCTTTGGTGGAGAGAAGA+AGG 0.424038 5_2:-20331593 None:intergenic
TACTAGTAGCTTAAATAGTT+AGG 0.424216 5_2:-20331510 None:intergenic
GGAAGAGGCTTTGTAGTTGT+AGG 0.424519 5_2:-20331544 None:intergenic
GTGATCTCATAATTCAAGTA+AGG 0.425248 5_2:+20332086 Msa0762310:CDS
TGCTTCTTCATCTTCAAAGA+TGG 0.426814 5_2:+20333267 Msa0762310:CDS
CTTGAACAACAACTCTTAAA+GGG 0.429190 5_2:-20334897 None:intergenic
TTGGTGTCAGTGACAAATCA+TGG 0.436046 5_2:+20335533 Msa0762310:three_prime_UTR
TTAACACATGATTTAGAAGA+AGG 0.437116 5_2:+20331861 Msa0762310:five_prime_UTR
TGTCACATAGTCATTCTAGA+AGG 0.445951 5_2:+20332852 Msa0762310:CDS
AGCTCTACTACAAGTATTCA+AGG 0.447165 5_2:+20335045 Msa0762310:CDS
GCATCACCAATGAGATTTGA+AGG 0.447840 5_2:-20333019 None:intergenic
GGCATAAGCATTTGGACAGC+AGG 0.452818 5_2:-20332445 None:intergenic
GTGTCATGAAAAGTTGATAT+CGG 0.454136 5_2:+20335499 Msa0762310:three_prime_UTR
TTGTGTAGGAAGAGCACAAA+TGG 0.459576 5_2:-20335214 None:intergenic
TGAATTTGATGAAAATGGTT+TGG 0.459746 5_2:+20335152 Msa0762310:CDS
CAAGCTAGAGCAGCATCATC+TGG 0.460416 5_2:+20334934 Msa0762310:CDS
ATTTGGTGATGAGATGAATC+AGG 0.463874 5_2:-20332494 None:intergenic
TACCTTGAGATAAATGTCAA+TGG 0.464242 5_2:-20333695 None:intergenic
ACAATGATAGCAATCAAATC+TGG 0.464906 5_2:+20332986 Msa0762310:CDS
AACGATCTCGGAGGGCTAGT+TGG 0.466201 5_2:-20333439 None:intergenic
AAGGTTCCTTCAAATCTCAT+TGG 0.467784 5_2:+20333013 Msa0762310:CDS
TTCAATTCTTGCATCCTCCA+TGG 0.467888 5_2:+20332698 Msa0762310:CDS
TCAAAAGTCTTCAACGATCT+CGG 0.468557 5_2:-20333451 None:intergenic
GCTTGTGGATAGGTACCTTC+AGG 0.469743 5_2:+20333573 Msa0762310:CDS
ATGCTTTGAAGTTATTTCGC+TGG 0.473982 5_2:-20333143 None:intergenic
GAAAGTTTGAGAATCAAATC+AGG 0.487700 5_2:+20332905 Msa0762310:CDS
CAGTGTTCTCAGAGTGTATG+AGG 0.487734 5_2:-20331678 None:intergenic
CAAAGATAGAATTTCTTTGG+TGG 0.488846 5_2:-20331604 None:intergenic
AAGAAGGGTAGGACTTCAAT+TGG 0.492020 5_2:+20333297 Msa0762310:CDS
TGCGTTGTTGTATGTTTCGA+TGG 0.498679 5_2:-20335018 None:intergenic
AGAATCAAATCAGGAAGTAA+AGG 0.499839 5_2:+20332914 Msa0762310:CDS
TGACTGAATGGACATAACTT+AGG 0.499955 5_2:+20331441 Msa0762310:five_prime_UTR
TTCTGCTAAGCAAGCTCAAG+TGG 0.505949 5_2:-20331723 None:intergenic
TTGGAACAGTTCATGTTACA+AGG 0.510924 5_2:+20333406 Msa0762310:CDS
AATGGTTTGGTAAATGATGA+TGG 0.512979 5_2:+20335165 Msa0762310:CDS
ATTCTTGCATCCTCCATGGT+TGG 0.519380 5_2:+20332702 Msa0762310:CDS
CACCCGGAAAATCAGGAAGT+TGG 0.520809 5_2:-20332520 None:intergenic
ATTGAAAGTGGCAAAGCTTG+TGG 0.529767 5_2:+20333558 Msa0762310:CDS
GCGAAATAACTTCAAAGCAT+AGG 0.531559 5_2:+20333146 Msa0762310:CDS
TGGTGTCAGTGACAAATCAT+GGG 0.535315 5_2:+20335534 Msa0762310:three_prime_UTR
CTTGCAGCTACAATGTTGTA+AGG 0.538418 5_2:-20332602 None:intergenic
TGCATCTGCTGGTAATTCCC+AGG 0.538781 5_2:-20332781 None:intergenic
AATAGTTTCAATGCATCTGC+TGG 0.540399 5_2:-20332792 None:intergenic
TTGCATCCTCCATGGTTGGA+AGG 0.541728 5_2:+20332706 Msa0762310:CDS
ACATAGTTCAAAATTGAAAG+TGG 0.541920 5_2:+20333546 Msa0762310:CDS
GTTTGTAAGGGTAATTTAGA+GGG 0.542040 5_2:+20335326 Msa0762310:exon
TTGAATTACAAGAGAGTAGG+AGG 0.548073 5_2:+20333509 Msa0762310:CDS
ACTTTGAATTACAAGAGAGT+AGG 0.548128 5_2:+20333506 Msa0762310:CDS
TCCAGATACTTTCTACACTT+CGG 0.550104 5_2:+20331926 Msa0762310:CDS
AGCCATTGACATTTATCTCA+AGG 0.550508 5_2:+20333693 Msa0762310:CDS
TGATCTCATAATTCAAGTAA+GGG 0.550839 5_2:+20332087 Msa0762310:CDS
AATGGAAGCGTGCATGCATG+CGG 0.551671 5_2:+20334858 Msa0762310:CDS
GTGACAAACTCACCTTTGTA+GGG 0.551753 5_2:-20332835 None:intergenic
TTGTGTGCTAAGTTCTGCTA+TGG 0.551870 5_2:+20332560 Msa0762310:CDS
CAAAGATGGAATTAGCAAGA+AGG 0.552149 5_2:+20333281 Msa0762310:CDS
CAAGAGGAAAGGTAAGGAAG+AGG 0.554998 5_2:-20331559 None:intergenic
AAACTTGATTCACAAGCTTG+AGG 0.555629 5_2:+20332670 Msa0762310:CDS
AAAGATGGAATTAGCAAGAA+GGG 0.557589 5_2:+20333282 Msa0762310:CDS
TTACCAAATATTTCCAAGAA+CGG 0.560310 5_2:+20333067 Msa0762310:CDS
CAGAACTCAACAAGAGTGAA+AGG 0.560948 5_2:+20334797 Msa0762310:CDS
GATCTCGGAGGGCTAGTTGG+TGG 0.563074 5_2:-20333436 None:intergenic
TGAACTATGTGATGCTGAAG+AGG 0.563744 5_2:-20333533 None:intergenic
ATAGTCATTCTAGAAGGATG+AGG 0.566210 5_2:+20332858 Msa0762310:CDS
CATACATTAAAAGATTCTTG+TGG 0.570521 5_2:-20335410 None:intergenic
TGTATAATTTGCAAGAGGAA+AGG 0.570984 5_2:-20331570 None:intergenic
GCATAAGCATTTGGACAGCA+GGG 0.571856 5_2:-20332444 None:intergenic
TCAAATGCTTCCACTCCACC+CGG 0.574131 5_2:-20332536 None:intergenic
CTATGATATCCTTCCAACCA+TGG 0.575719 5_2:-20332715 None:intergenic
GAAGGTGTATAATTTGCAAG+AGG 0.578392 5_2:-20331575 None:intergenic
ATCAGGAAGTAAAGGATGGT+GGG 0.580568 5_2:+20332922 Msa0762310:CDS
ATGGAATTAGCAAGAAGGGT+AGG 0.583335 5_2:+20333286 Msa0762310:CDS
AGCAATCAAATCTGGTGGCA+AGG 0.587341 5_2:+20332994 Msa0762310:CDS
CATCTCTTGCAACTTCCTGA+AGG 0.592567 5_2:-20333588 None:intergenic
AAAAGCGTTGCATTTGTGGT+CGG 0.592600 5_2:+20332754 Msa0762310:CDS
GTCTGAGTCTAACTCAGACT+CGG 0.598165 5_2:+20333120 Msa0762310:CDS
AATCAGGAAGTAAAGGATGG+TGG 0.603373 5_2:+20332921 Msa0762310:CDS
TACTACAAGTATTCAAGGTG+AGG 0.607107 5_2:+20335050 Msa0762310:CDS
TCTACACTGAATTATGCAAG+TGG 0.608488 5_2:+20331827 Msa0762310:five_prime_UTR
AAGGGTAGGACTTCAATTGG+AGG 0.609288 5_2:+20333300 Msa0762310:CDS
AGTCTTGAGCCACCCTACAA+AGG 0.609787 5_2:+20332823 Msa0762310:CDS
TAGTCATTCTAGAAGGATGA+GGG 0.613006 5_2:+20332859 Msa0762310:CDS
TCAAATCAGGAAGTAAAGGA+TGG 0.616909 5_2:+20332918 Msa0762310:CDS
TACAGTCTATGATGTTGAGT+TGG 0.618402 5_2:+20333372 Msa0762310:CDS
AATTTGCAAGAGGAAAGGTA+AGG 0.621417 5_2:-20331565 None:intergenic
AAGTCTTCAACGATCTCGGA+GGG 0.622487 5_2:-20333447 None:intergenic
AAGTGGCAAAGCTTGTGGAT+AGG 0.625893 5_2:+20333563 Msa0762310:CDS
ATGATAGCAATCAAATCTGG+TGG 0.634663 5_2:+20332989 Msa0762310:CDS
GCTAGAGCAGCATCATCTGG+TGG 0.637791 5_2:+20334937 Msa0762310:CDS
GATCTCATAATTCAAGTAAG+GGG 0.642036 5_2:+20332088 Msa0762310:CDS
GTCTGAGTTAGACTCAGACT+CGG 0.643618 5_2:-20333116 None:intergenic
TCTAAGAATGAAGCTTGCTG+AGG 0.644686 5_2:+20335122 Msa0762310:CDS
AAAGCATTGCTTACAACACA+TGG 0.648620 5_2:+20334988 Msa0762310:CDS
ATACACTCTGAGAACACTGT+CGG 0.653041 5_2:+20331682 Msa0762310:five_prime_UTR
CATTTGTGGTCGGAAGACCT+GGG 0.654710 5_2:+20332764 Msa0762310:CDS
AAAGTCTTCAACGATCTCGG+AGG 0.669614 5_2:-20333448 None:intergenic
CATAAGCATTTGGACAGCAG+GGG 0.670987 5_2:-20332443 None:intergenic
ACAAACTCACCTTTGTAGGG+TGG 0.674969 5_2:-20332832 None:intergenic
TCATTTGTATAAGAAAGTGA+AGG 0.677583 5_2:-20333349 None:intergenic
AAGTAGATATCTTCTTCACA+AGG 0.678976 5_2:+20332111 Msa0762310:CDS
GGTGTCAGTGACAAATCATG+GGG 0.687341 5_2:+20335535 Msa0762310:three_prime_UTR
TGATATCCTTCCAACCATGG+AGG 0.717676 5_2:-20332712 None:intergenic
TGGTGATGAGATGAATCAGG+AGG 0.725233 5_2:-20332491 None:intergenic
TATTAATAGATCATTCACTG+TGG 0.782652 5_2:-20333326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTATATACTTATGA+AGG - chr5_2:20332379-20332398 None:intergenic 10.0%
!!! AAGTATATAATTTTTTACTT+TGG + chr5_2:20332383-20332402 Msa0762310:intron 10.0%
!!! AAATACAATAAACATTTTAT+AGG - chr5_2:20333730-20333749 None:intergenic 10.0%
!!! TAATTTAAGTTTTTTTTTCA+AGG + chr5_2:20334397-20334416 Msa0762310:intron 10.0%
!! CTCAAAATTATTATTATTAT+AGG - chr5_2:20335452-20335471 None:intergenic 10.0%
!!! CTATAATAATAATAATTTTG+AGG + chr5_2:20335450-20335469 Msa0762310:three_prime_UTR 10.0%
!! AAAATAAATATTCTTCATCT+TGG + chr5_2:20331745-20331764 Msa0762310:five_prime_UTR 15.0%
!!! ATTTTTACCAAGTTATAAAA+TGG - chr5_2:20333765-20333784 None:intergenic 15.0%
!!! TTATGTGTGTATTTTTTTAT+AGG - chr5_2:20333919-20333938 None:intergenic 15.0%
!! AAAACATGTAAAAAAAAAAG+TGG + chr5_2:20333939-20333958 Msa0762310:intron 15.0%
!!! TTGAATAAGACAATATTTTA+AGG - chr5_2:20334038-20334057 None:intergenic 15.0%
!!! AGTAATGTAATTTTAGAGAA+TGG - chr5_2:20331371-20331390 None:intergenic 20.0%
!! TAAAATGTCAAAATCACTTA+AGG + chr5_2:20331407-20331426 Msa0762310:five_prime_UTR 20.0%
!!! TTTTCTTTTTACTATCTGTT+AGG + chr5_2:20331785-20331804 Msa0762310:five_prime_UTR 20.0%
!! CAAAATTAAACAATCCAAAA+AGG - chr5_2:20332013-20332032 None:intergenic 20.0%
!!! ATACTTCACTTTCAAATTTT+AGG + chr5_2:20333975-20333994 Msa0762310:intron 20.0%
!!! TTGTCTTATTCAACATTTAT+TGG + chr5_2:20334045-20334064 Msa0762310:intron 20.0%
!! AAATAGAGTGAATTCATAAA+TGG - chr5_2:20334295-20334314 None:intergenic 20.0%
!! ATATAGGACTAATAAAAACT+AGG + chr5_2:20334495-20334514 Msa0762310:intron 20.0%
!!! CAATTTACTAAGCATTTTTT+AGG - chr5_2:20335240-20335259 None:intergenic 20.0%
!! AAAAAATGCTTAGTAAATTG+TGG + chr5_2:20335240-20335259 Msa0762310:CDS 20.0%
!!! TTAGTTTTTTCATGTTTGTA+AGG + chr5_2:20335313-20335332 Msa0762310:CDS 20.0%
!!! TAGTTTTTTCATGTTTGTAA+GGG + chr5_2:20335314-20335333 Msa0762310:CDS 20.0%
! GTTATAACTATTTGACTGAA+TGG + chr5_2:20331429-20331448 Msa0762310:five_prime_UTR 25.0%
! TACTAGTAGCTTAAATAGTT+AGG - chr5_2:20331513-20331532 None:intergenic 25.0%
! AAGCAAAGATAGAATTTCTT+TGG - chr5_2:20331610-20331629 None:intergenic 25.0%
! AAGAAAAGTTCAGAATTCTT+AGG - chr5_2:20331653-20331672 None:intergenic 25.0%
! TTAACACATGATTTAGAAGA+AGG + chr5_2:20331861-20331880 Msa0762310:five_prime_UTR 25.0%
! TGATCTCATAATTCAAGTAA+GGG + chr5_2:20332087-20332106 Msa0762310:CDS 25.0%
! ACTAATATGACACATTGATA+CGG - chr5_2:20332308-20332327 None:intergenic 25.0%
! TGAAAATTTATGCATCTAGA+TGG + chr5_2:20333044-20333063 Msa0762310:CDS 25.0%
! TTACCAAATATTTCCAAGAA+CGG + chr5_2:20333067-20333086 Msa0762310:CDS 25.0%
! TATTAATAGATCATTCACTG+TGG - chr5_2:20333329-20333348 None:intergenic 25.0%
! TCATTTGTATAAGAAAGTGA+AGG - chr5_2:20333352-20333371 None:intergenic 25.0%
! ACATAGTTCAAAATTGAAAG+TGG + chr5_2:20333546-20333565 Msa0762310:CDS 25.0%
! TGGTAAAAATATATCTCAGA+TGG + chr5_2:20333775-20333794 Msa0762310:intron 25.0%
! TTCAACATTTATTGGAAGTT+AGG + chr5_2:20334053-20334072 Msa0762310:intron 25.0%
! AGATAATTTCATAACCCATT+TGG + chr5_2:20334149-20334168 Msa0762310:intron 25.0%
! CTACCATGTTCAATTAATAT+AGG + chr5_2:20334479-20334498 Msa0762310:intron 25.0%
! AGTCCTATATTAATTGAACA+TGG - chr5_2:20334485-20334504 None:intergenic 25.0%
!! AGAAAATTTTCTATAGCATG+AGG + chr5_2:20334525-20334544 Msa0762310:intron 25.0%
! AAAATAACGAGCTTGAAATA+TGG - chr5_2:20334641-20334660 None:intergenic 25.0%
!! TTTCTTAATTTGAAACATGC+AGG + chr5_2:20334768-20334787 Msa0762310:intron 25.0%
! CATGATGAATTTGATGAAAA+TGG + chr5_2:20335147-20335166 Msa0762310:CDS 25.0%
! TGAATTTGATGAAAATGGTT+TGG + chr5_2:20335152-20335171 Msa0762310:CDS 25.0%
! CATACATTAAAAGATTCTTG+TGG - chr5_2:20335413-20335432 None:intergenic 25.0%
!! AATCTTTTAATGTATGTGCA+TGG + chr5_2:20335417-20335436 Msa0762310:three_prime_UTR 25.0%
GTTAGGATAGATGACATTAA+TGG - chr5_2:20331496-20331515 None:intergenic 30.0%
! TGTATAATTTGCAAGAGGAA+AGG - chr5_2:20331573-20331592 None:intergenic 30.0%
CAAAGATAGAATTTCTTTGG+TGG - chr5_2:20331607-20331626 None:intergenic 30.0%
TCCAAGTTTCATAAACTTCA+TGG - chr5_2:20331901-20331920 None:intergenic 30.0%
! GTTCTTTTCTTTGTATATGC+AGG + chr5_2:20332041-20332060 Msa0762310:intron 30.0%
GTGATCTCATAATTCAAGTA+AGG + chr5_2:20332086-20332105 Msa0762310:CDS 30.0%
GATCTCATAATTCAAGTAAG+GGG + chr5_2:20332088-20332107 Msa0762310:CDS 30.0%
AAGTAGATATCTTCTTCACA+AGG + chr5_2:20332111-20332130 Msa0762310:CDS 30.0%
!! TGTCATATTAGTGTTGTTTC+TGG + chr5_2:20332316-20332335 Msa0762310:intron 30.0%
CACAAACACAAATACAAACA+CGG - chr5_2:20332345-20332364 None:intergenic 30.0%
TTTCAACTTCTTCAGTCATT+TGG - chr5_2:20332646-20332665 None:intergenic 30.0%
ATGACTGAAGAAGTTGAAAA+AGG + chr5_2:20332647-20332666 Msa0762310:CDS 30.0%
GAAAGTTTGAGAATCAAATC+AGG + chr5_2:20332905-20332924 Msa0762310:CDS 30.0%
AGAATCAAATCAGGAAGTAA+AGG + chr5_2:20332914-20332933 Msa0762310:CDS 30.0%
AACTTAGCATAGACCTTTAT+TGG + chr5_2:20332960-20332979 Msa0762310:CDS 30.0%
CTATCATTGTTCTCCAATAA+AGG - chr5_2:20332976-20332995 None:intergenic 30.0%
ACAATGATAGCAATCAAATC+TGG + chr5_2:20332986-20333005 Msa0762310:CDS 30.0%
TTTCCGTTCTTGGAAATATT+TGG - chr5_2:20333073-20333092 None:intergenic 30.0%
!! TGAAGAAGCATTGAGAATAT+TGG - chr5_2:20333257-20333276 None:intergenic 30.0%
AAAGATGGAATTAGCAAGAA+GGG + chr5_2:20333282-20333301 Msa0762310:CDS 30.0%
ACTTTGAATTACAAGAGAGT+AGG + chr5_2:20333506-20333525 Msa0762310:CDS 30.0%
TGATCATATCTTGCAAATTC+TGG - chr5_2:20333658-20333677 None:intergenic 30.0%
TACCTTGAGATAAATGTCAA+TGG - chr5_2:20333698-20333717 None:intergenic 30.0%
! AGTCACTCCATTTTATAACT+TGG + chr5_2:20333755-20333774 Msa0762310:intron 30.0%
AGGATATGAAACAGAATTAG+TGG + chr5_2:20333995-20334014 Msa0762310:intron 30.0%
!!! TATTTTAAGGTCAAGAGAGT+TGG - chr5_2:20334025-20334044 None:intergenic 30.0%
CAACTATATCAGTCAAGATA+AGG - chr5_2:20334117-20334136 None:intergenic 30.0%
CATCATTATAGAGTTCATTG+AGG - chr5_2:20334330-20334349 None:intergenic 30.0%
!! ACACTGAATGAGTTCTAAAT+AGG - chr5_2:20334439-20334458 None:intergenic 30.0%
ATTAATTGAACATGGTAGCT+CGG - chr5_2:20334477-20334496 None:intergenic 30.0%
GACTAATAAAAACTAGGCTA+TGG + chr5_2:20334501-20334520 Msa0762310:intron 30.0%
CTTGAACAACAACTCTTAAA+GGG - chr5_2:20334900-20334919 None:intergenic 30.0%
ACTTGAACAACAACTCTTAA+AGG - chr5_2:20334901-20334920 None:intergenic 30.0%
!! AGAGTTGAAGACTTTGATTT+TGG - chr5_2:20335105-20335124 None:intergenic 30.0%
!! AATGGTTTGGTAAATGATGA+TGG + chr5_2:20335165-20335184 Msa0762310:CDS 30.0%
!! TTGGTAAATGATGATGGAAT+TGG + chr5_2:20335171-20335190 Msa0762310:CDS 30.0%
!!! AAGCATTTTTTAGGTTGTGT+AGG - chr5_2:20335231-20335250 None:intergenic 30.0%
TGTTTGTAAGGGTAATTTAG+AGG + chr5_2:20335325-20335344 Msa0762310:CDS 30.0%
GTTTGTAAGGGTAATTTAGA+GGG + chr5_2:20335326-20335345 Msa0762310:exon 30.0%
!! ATTTTGAGGGAAAATCTCTT+TGG + chr5_2:20335464-20335483 Msa0762310:three_prime_UTR 30.0%
!! GTGTCATGAAAAGTTGATAT+CGG + chr5_2:20335499-20335518 Msa0762310:three_prime_UTR 30.0%
!! TGAAAAGTTGATATCGGAAT+AGG + chr5_2:20335505-20335524 Msa0762310:three_prime_UTR 30.0%
GTAGACAAATACAGCAATAT+TGG - chr5_2:20335561-20335580 None:intergenic 30.0%
! AATTCGTATTTTCATGTGTC+TGG + chr5_2:20335611-20335630 Msa0762310:three_prime_UTR 30.0%
TGACTGAATGGACATAACTT+AGG + chr5_2:20331441-20331460 Msa0762310:five_prime_UTR 35.0%
AATTTGCAAGAGGAAAGGTA+AGG - chr5_2:20331568-20331587 None:intergenic 35.0%
! GAAGGTGTATAATTTGCAAG+AGG - chr5_2:20331578-20331597 None:intergenic 35.0%
TCTACACTGAATTATGCAAG+TGG + chr5_2:20331827-20331846 Msa0762310:five_prime_UTR 35.0%
GCCATGAAGTTTATGAAACT+TGG + chr5_2:20331897-20331916 Msa0762310:exon 35.0%
TCCAGATACTTTCTACACTT+CGG + chr5_2:20331926-20331945 Msa0762310:CDS 35.0%
! TAGATGATGCATCTCCTTTT+TGG + chr5_2:20331996-20332015 Msa0762310:intron 35.0%
ACGTATTGTTACATTTGGGA+AGG + chr5_2:20332260-20332279 Msa0762310:intron 35.0%
ATTTGTGTTTGTGTCTGTGT+CGG + chr5_2:20332352-20332371 Msa0762310:intron 35.0%
ATTCTGAGCATAATCTTTGC+AGG - chr5_2:20332469-20332488 None:intergenic 35.0%
!! ATTTGGTGATGAGATGAATC+AGG - chr5_2:20332497-20332516 None:intergenic 35.0%
AATCAGGAAGTTGGACTATT+TGG - chr5_2:20332514-20332533 None:intergenic 35.0%
AAACTTGATTCACAAGCTTG+AGG + chr5_2:20332670-20332689 Msa0762310:CDS 35.0%
AATAGTTTCAATGCATCTGC+TGG - chr5_2:20332795-20332814 None:intergenic 35.0%
TGTCACATAGTCATTCTAGA+AGG + chr5_2:20332852-20332871 Msa0762310:CDS 35.0%
! ATAGTCATTCTAGAAGGATG+AGG + chr5_2:20332858-20332877 Msa0762310:CDS 35.0%
! TAGTCATTCTAGAAGGATGA+GGG + chr5_2:20332859-20332878 Msa0762310:CDS 35.0%
TCAAATCAGGAAGTAAAGGA+TGG + chr5_2:20332918-20332937 Msa0762310:CDS 35.0%
ATGATAGCAATCAAATCTGG+TGG + chr5_2:20332989-20333008 Msa0762310:CDS 35.0%
AAGGTTCCTTCAAATCTCAT+TGG + chr5_2:20333013-20333032 Msa0762310:CDS 35.0%
CTTCTTTCTATTTCCGTTCT+TGG - chr5_2:20333083-20333102 None:intergenic 35.0%
!! ATGCTTTGAAGTTATTTCGC+TGG - chr5_2:20333146-20333165 None:intergenic 35.0%
GCGAAATAACTTCAAAGCAT+AGG + chr5_2:20333146-20333165 Msa0762310:CDS 35.0%
TTCAAAGCATAGGCTTCTAT+TGG + chr5_2:20333156-20333175 Msa0762310:CDS 35.0%
TGCTTCTTCATCTTCAAAGA+TGG + chr5_2:20333267-20333286 Msa0762310:CDS 35.0%
CAAAGATGGAATTAGCAAGA+AGG + chr5_2:20333281-20333300 Msa0762310:CDS 35.0%
TACAGTCTATGATGTTGAGT+TGG + chr5_2:20333372-20333391 Msa0762310:CDS 35.0%
!! TGAGTTGGTGATGACTATAT+TGG + chr5_2:20333387-20333406 Msa0762310:CDS 35.0%
TTGGAACAGTTCATGTTACA+AGG + chr5_2:20333406-20333425 Msa0762310:CDS 35.0%
TCAAAAGTCTTCAACGATCT+CGG - chr5_2:20333454-20333473 None:intergenic 35.0%
!! CGTTGAAGACTTTTGAAAGA+AGG + chr5_2:20333458-20333477 Msa0762310:CDS 35.0%
TTGAATTACAAGAGAGTAGG+AGG + chr5_2:20333509-20333528 Msa0762310:CDS 35.0%
AGCCATTGACATTTATCTCA+AGG + chr5_2:20333693-20333712 Msa0762310:CDS 35.0%
TCTGTCTTAATACTCGTTGA+GGG - chr5_2:20333826-20333845 None:intergenic 35.0%
ATTAGTAGTTCGTTACGAGA+GGG - chr5_2:20333884-20333903 None:intergenic 35.0%
CATTTATTGGAAGTTAGGTG+TGG + chr5_2:20334058-20334077 Msa0762310:intron 35.0%
!!! CTTCACGTTTTTAGCCAAAT+GGG - chr5_2:20334166-20334185 None:intergenic 35.0%
!!! TCTTCACGTTTTTAGCCAAA+TGG - chr5_2:20334167-20334186 None:intergenic 35.0%
! ACATAGTACTGGAACTATGA+TGG - chr5_2:20334220-20334239 None:intergenic 35.0%
CACACTATCAATTCATGAGA+AGG - chr5_2:20334243-20334262 None:intergenic 35.0%
CATTATAGAGTTCATTGAGG+TGG - chr5_2:20334327-20334346 None:intergenic 35.0%
!! CTGAATGAGTTCTAAATAGG+AGG - chr5_2:20334436-20334455 None:intergenic 35.0%
ATACGTGAATATGTGACTAC+TGG + chr5_2:20334702-20334721 Msa0762310:intron 35.0%
!! AAAGCATTGCTTACAACACA+TGG + chr5_2:20334988-20335007 Msa0762310:CDS 35.0%
AGCTCTACTACAAGTATTCA+AGG + chr5_2:20335045-20335064 Msa0762310:CDS 35.0%
TACTACAAGTATTCAAGGTG+AGG + chr5_2:20335050-20335069 Msa0762310:CDS 35.0%
GTATTCAAGGTGAGGATAAT+TGG + chr5_2:20335058-20335077 Msa0762310:CDS 35.0%
! CAAAAAGCCTTGTAACTTGT+AGG + chr5_2:20335382-20335401 Msa0762310:three_prime_UTR 35.0%
! GGATTTTCCTACAAGTTACA+AGG - chr5_2:20335392-20335411 None:intergenic 35.0%
GATATCGGAATAGGAATCTT+TGG + chr5_2:20335514-20335533 Msa0762310:three_prime_UTR 35.0%
! TTTTGTCAAGACACAAGTGT+TGG - chr5_2:20335590-20335609 None:intergenic 35.0%
TTTCTTTGGTGGAGAGAAGA+AGG - chr5_2:20331596-20331615 None:intergenic 40.0%
! ATACACTCTGAGAACACTGT+CGG + chr5_2:20331682-20331701 Msa0762310:five_prime_UTR 40.0%
!! TCCGAAGTGTAGAAAGTATC+TGG - chr5_2:20331930-20331949 None:intergenic 40.0%
TCTACACTTCGGAAGCTATA+AGG + chr5_2:20331937-20331956 Msa0762310:CDS 40.0%
! GTACTGACGCTTCAAATTGA+AGG + chr5_2:20332206-20332225 Msa0762310:intron 40.0%
CCAAATGTAACAATACGTGC+CGG - chr5_2:20332258-20332277 None:intergenic 40.0%
CGGCACGTATTGTTACATTT+GGG + chr5_2:20332256-20332275 Msa0762310:intron 40.0%
TCTTTGCAGGCATAAGCATT+TGG - chr5_2:20332456-20332475 None:intergenic 40.0%
! AGTCCAACTTCCTGATTTTC+CGG + chr5_2:20332517-20332536 Msa0762310:CDS 40.0%
TTGTGTGCTAAGTTCTGCTA+TGG + chr5_2:20332560-20332579 Msa0762310:CDS 40.0%
CTTGCAGCTACAATGTTGTA+AGG - chr5_2:20332605-20332624 None:intergenic 40.0%
TTCAATTCTTGCATCCTCCA+TGG + chr5_2:20332698-20332717 Msa0762310:CDS 40.0%
CTATGATATCCTTCCAACCA+TGG - chr5_2:20332718-20332737 None:intergenic 40.0%
!! AAAAGCGTTGCATTTGTGGT+CGG + chr5_2:20332754-20332773 Msa0762310:CDS 40.0%
GTGACAAACTCACCTTTGTA+GGG - chr5_2:20332838-20332857 None:intergenic 40.0%
TGTGACAAACTCACCTTTGT+AGG - chr5_2:20332839-20332858 None:intergenic 40.0%
AATCAGGAAGTAAAGGATGG+TGG + chr5_2:20332921-20332940 Msa0762310:CDS 40.0%
ATCAGGAAGTAAAGGATGGT+GGG + chr5_2:20332922-20332941 Msa0762310:CDS 40.0%
GCATCACCAATGAGATTTGA+AGG - chr5_2:20333022-20333041 None:intergenic 40.0%
! AGCTTGCTTCCAACAGAAAA+AGG + chr5_2:20333190-20333209 Msa0762310:CDS 40.0%
ATGGAATTAGCAAGAAGGGT+AGG + chr5_2:20333286-20333305 Msa0762310:CDS 40.0%
AAGAAGGGTAGGACTTCAAT+TGG + chr5_2:20333297-20333316 Msa0762310:CDS 40.0%
TGAACTATGTGATGCTGAAG+AGG - chr5_2:20333536-20333555 None:intergenic 40.0%
ATTGAAAGTGGCAAAGCTTG+TGG + chr5_2:20333558-20333577 Msa0762310:CDS 40.0%
CTCTGTCTTAATACTCGTTG+AGG - chr5_2:20333827-20333846 None:intergenic 40.0%
CTCAACGAGTATTAAGACAG+AGG + chr5_2:20333825-20333844 Msa0762310:intron 40.0%
TTAATAGGAGGGATTAAGCG+CGG - chr5_2:20333853-20333872 None:intergenic 40.0%
TTAGTAGTTCGTTACGAGAG+GGG - chr5_2:20333883-20333902 None:intergenic 40.0%
GATTAGTAGTTCGTTACGAG+AGG - chr5_2:20333885-20333904 None:intergenic 40.0%
!! GTCAGGCTAAAATTTCTGGT+GGG - chr5_2:20334089-20334108 None:intergenic 40.0%
! AGTCAGGCTAAAATTTCTGG+TGG - chr5_2:20334090-20334109 None:intergenic 40.0%
GTCAGTCAGGCTAAAATTTC+TGG - chr5_2:20334093-20334112 None:intergenic 40.0%
TCATGAGAAGGACATAGTAC+TGG - chr5_2:20334231-20334250 None:intergenic 40.0%
GAGTTCATTGAGGTGGAAAT+TGG - chr5_2:20334320-20334339 None:intergenic 40.0%
!! GAGTTCTAAATAGGAGGTTC+TGG - chr5_2:20334430-20334449 None:intergenic 40.0%
! TGAGGCGTCACTATAATGAT+TGG + chr5_2:20334543-20334562 Msa0762310:intron 40.0%
CTTTCACTCTTGTTGAGTTC+TGG - chr5_2:20334799-20334818 None:intergenic 40.0%
CAGAACTCAACAAGAGTGAA+AGG + chr5_2:20334797-20334816 Msa0762310:CDS 40.0%
TGACTGCAAAAAGCTCTCAA+TGG + chr5_2:20334840-20334859 Msa0762310:CDS 40.0%
TGCGTTGTTGTATGTTTCGA+TGG - chr5_2:20335021-20335040 None:intergenic 40.0%
! GAGGATAATTGGAGTGTTTC+TGG + chr5_2:20335069-20335088 Msa0762310:CDS 40.0%
!! TCTAAGAATGAAGCTTGCTG+AGG + chr5_2:20335122-20335141 Msa0762310:CDS 40.0%
! TTGTGTAGGAAGAGCACAAA+TGG - chr5_2:20335217-20335236 None:intergenic 40.0%
!! TTGGTGTCAGTGACAAATCA+TGG + chr5_2:20335533-20335552 Msa0762310:three_prime_UTR 40.0%
! TGGTGTCAGTGACAAATCAT+GGG + chr5_2:20335534-20335553 Msa0762310:three_prime_UTR 40.0%
! GGAAGAGGCTTTGTAGTTGT+AGG - chr5_2:20331547-20331566 None:intergenic 45.0%
CAAGAGGAAAGGTAAGGAAG+AGG - chr5_2:20331562-20331581 None:intergenic 45.0%
CAGTGTTCTCAGAGTGTATG+AGG - chr5_2:20331681-20331700 None:intergenic 45.0%
TTCTGCTAAGCAAGCTCAAG+TGG - chr5_2:20331726-20331745 None:intergenic 45.0%
CCGGCACGTATTGTTACATT+TGG + chr5_2:20332255-20332274 Msa0762310:intron 45.0%
CATAAGCATTTGGACAGCAG+GGG - chr5_2:20332446-20332465 None:intergenic 45.0%
GCATAAGCATTTGGACAGCA+GGG - chr5_2:20332447-20332466 None:intergenic 45.0%
! TGGTGATGAGATGAATCAGG+AGG - chr5_2:20332494-20332513 None:intergenic 45.0%
! GTCCAACTTCCTGATTTTCC+GGG + chr5_2:20332518-20332537 Msa0762310:CDS 45.0%
ATTCTTGCATCCTCCATGGT+TGG + chr5_2:20332702-20332721 Msa0762310:CDS 45.0%
TGATATCCTTCCAACCATGG+AGG - chr5_2:20332715-20332734 None:intergenic 45.0%
! AATGCAACGCTTTTGTGGTC+TGG - chr5_2:20332748-20332767 None:intergenic 45.0%
! CCACAAATGCAACGCTTTTG+TGG - chr5_2:20332753-20332772 None:intergenic 45.0%
!! CCACAAAAGCGTTGCATTTG+TGG + chr5_2:20332750-20332769 Msa0762310:CDS 45.0%
ACAAACTCACCTTTGTAGGG+TGG - chr5_2:20332835-20332854 None:intergenic 45.0%
AGCAATCAAATCTGGTGGCA+AGG + chr5_2:20332994-20333013 Msa0762310:CDS 45.0%
GTCTGAGTTAGACTCAGACT+CGG - chr5_2:20333119-20333138 None:intergenic 45.0%
GTCTGAGTCTAACTCAGACT+CGG + chr5_2:20333120-20333139 Msa0762310:CDS 45.0%
! GAAACAGCGCCTTTTTCTGT+TGG - chr5_2:20333202-20333221 None:intergenic 45.0%
AAGGGTAGGACTTCAATTGG+AGG + chr5_2:20333300-20333319 Msa0762310:CDS 45.0%
AAGTCTTCAACGATCTCGGA+GGG - chr5_2:20333450-20333469 None:intergenic 45.0%
AAAGTCTTCAACGATCTCGG+AGG - chr5_2:20333451-20333470 None:intergenic 45.0%
TGAAAGAAGGCGATCACGTT+CGG + chr5_2:20333471-20333490 Msa0762310:CDS 45.0%
AAGTGGCAAAGCTTGTGGAT+AGG + chr5_2:20333563-20333582 Msa0762310:CDS 45.0%
CATCTCTTGCAACTTCCTGA+AGG - chr5_2:20333591-20333610 None:intergenic 45.0%
TGTAGCTCAGTGATACGAGT+TGG + chr5_2:20333798-20333817 Msa0762310:intron 45.0%
GTAGCTCAGTGATACGAGTT+GGG + chr5_2:20333799-20333818 Msa0762310:intron 45.0%
GGGGGAAATCGTTAATAGGA+GGG - chr5_2:20333864-20333883 None:intergenic 45.0%
AGGGGGAAATCGTTAATAGG+AGG - chr5_2:20333865-20333884 None:intergenic 45.0%
GAGAGGGGGAAATCGTTAAT+AGG - chr5_2:20333868-20333887 None:intergenic 45.0%
TAGTAGTTCGTTACGAGAGG+GGG - chr5_2:20333882-20333901 None:intergenic 45.0%
GTCAAGATAAGGAGTCAGTC+AGG - chr5_2:20334106-20334125 None:intergenic 45.0%
ATTGAACATGGTAGCTCGGT+TGG - chr5_2:20334473-20334492 None:intergenic 45.0%
GTCAGTTCATTGGTAGGTGT+TGG + chr5_2:20334599-20334618 Msa0762310:intron 45.0%
!! GGTGTTGGCTCAAAATTCGA+CGG + chr5_2:20334614-20334633 Msa0762310:intron 45.0%
GGTGTCAGTGACAAATCATG+GGG + chr5_2:20335535-20335554 Msa0762310:three_prime_UTR 45.0%
!!! ATGTTTTTTTATTTTTATTT+TGG + chr5_2:20334354-20334373 Msa0762310:intron 5.0%
!!! TATAATAATAATAATTTTGA+GGG + chr5_2:20335451-20335470 Msa0762310:three_prime_UTR 5.0%
GTCAGACACTTACACGACAC+CGG + chr5_2:20332236-20332255 Msa0762310:intron 50.0%
GGCATAAGCATTTGGACAGC+AGG - chr5_2:20332448-20332467 None:intergenic 50.0%
CACCCGGAAAATCAGGAAGT+TGG - chr5_2:20332523-20332542 None:intergenic 50.0%
! CAACTTCCTGATTTTCCGGG+TGG + chr5_2:20332521-20332540 Msa0762310:CDS 50.0%
TCAAATGCTTCCACTCCACC+CGG - chr5_2:20332539-20332558 None:intergenic 50.0%
TTGCATCCTCCATGGTTGGA+AGG + chr5_2:20332706-20332725 Msa0762310:CDS 50.0%
CATTTGTGGTCGGAAGACCT+GGG + chr5_2:20332764-20332783 Msa0762310:CDS 50.0%
TGCATCTGCTGGTAATTCCC+AGG - chr5_2:20332784-20332803 None:intergenic 50.0%
AGTCTTGAGCCACCCTACAA+AGG + chr5_2:20332823-20332842 Msa0762310:CDS 50.0%
GCTTGTGGATAGGTACCTTC+AGG + chr5_2:20333573-20333592 Msa0762310:CDS 50.0%
CATTCGCGCAGTCAGTTCAT+TGG + chr5_2:20334589-20334608 Msa0762310:intron 50.0%
! AATGGAAGCGTGCATGCATG+CGG + chr5_2:20334858-20334877 Msa0762310:CDS 50.0%
CAAGCTAGAGCAGCATCATC+TGG + chr5_2:20334934-20334953 Msa0762310:CDS 50.0%
! TCCTGATTTTCCGGGTGGAG+TGG + chr5_2:20332526-20332545 Msa0762310:CDS 55.0%
TCCACTCCACCCGGAAAATC+AGG - chr5_2:20332530-20332549 None:intergenic 55.0%
GCATTTGTGGTCGGAAGACC+TGG + chr5_2:20332763-20332782 Msa0762310:CDS 55.0%
! AACGATCTCGGAGGGCTAGT+TGG - chr5_2:20333442-20333461 None:intergenic 55.0%
! ACATGGTAGCTCGGTTGGTG+AGG - chr5_2:20334468-20334487 None:intergenic 55.0%
CGCGCAGTCAGTTCATTGGT+AGG + chr5_2:20334593-20334612 Msa0762310:intron 55.0%
GCTAGAGCAGCATCATCTGG+TGG + chr5_2:20334937-20334956 Msa0762310:CDS 55.0%
!! GATCTCGGAGGGCTAGTTGG+TGG - chr5_2:20333439-20333458 None:intergenic 60.0%
GAACTGACTGCGCGAATGCG+AGG - chr5_2:20334587-20334606 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 20331342 20335655 20331342 ID=Msa0762310;Name=Msa0762310
chr5_2 mRNA 20331342 20335655 20331342 ID=Msa0762310-mRNA-1;Parent=Msa0762310;Name=Msa0762310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=558|1|1|1|1|1|4|311|652
chr5_2 exon 20331342 20331958 20331342 ID=Msa0762310-mRNA-1:exon:8576;Parent=Msa0762310-mRNA-1
chr5_2 exon 20332063 20332132 20332063 ID=Msa0762310-mRNA-1:exon:8577;Parent=Msa0762310-mRNA-1
chr5_2 exon 20332440 20333714 20332440 ID=Msa0762310-mRNA-1:exon:8578;Parent=Msa0762310-mRNA-1
chr5_2 exon 20334790 20335655 20334790 ID=Msa0762310-mRNA-1:exon:8579;Parent=Msa0762310-mRNA-1
chr5_2 five_prime_UTR 20331342 20331899 20331342 ID=Msa0762310-mRNA-1:five_prime_utr;Parent=Msa0762310-mRNA-1
chr5_2 CDS 20331900 20331958 20331900 ID=Msa0762310-mRNA-1:cds;Parent=Msa0762310-mRNA-1
chr5_2 CDS 20332063 20332132 20332063 ID=Msa0762310-mRNA-1:cds;Parent=Msa0762310-mRNA-1
chr5_2 CDS 20332440 20333714 20332440 ID=Msa0762310-mRNA-1:cds;Parent=Msa0762310-mRNA-1
chr5_2 CDS 20334790 20335344 20334790 ID=Msa0762310-mRNA-1:cds;Parent=Msa0762310-mRNA-1
chr5_2 three_prime_UTR 20335345 20335655 20335345 ID=Msa0762310-mRNA-1:three_prime_utr;Parent=Msa0762310-mRNA-1
Gene Sequence

>Msa0762310

ATGAAGTTTATGAAACTTGGATCTCGTCCAGATACTTTCTACACTTCGGAAGCTATAAGGACAATCTCTTCTGAAGTTTCTAGTGATCTCATAATTCAAGTAAGGGGAAGTAGATATCTTCTTCACAAGTTTCCCCTGCTGTCCAAATGCTTATGCCTGCAAAGATTATGCTCAGAATCACCTCCTGATTCATCTCATCACCAAATAGTCCAACTTCCTGATTTTCCGGGTGGAGTGGAAGCATTTGAGTTGTGTGCTAAGTTCTGCTATGGAATCACAATTACTTTAAGTCCTTACAACATTGTAGCTGCAAGATGTGCTGCTGAATATCTCCAAATGACTGAAGAAGTTGAAAAAGGAAACTTGATTCACAAGCTTGAGGTTTTTTTCAATTCTTGCATCCTCCATGGTTGGAAGGATATCATAGTAAGTCTCCAGACCACAAAAGCGTTGCATTTGTGGTCGGAAGACCTGGGAATTACCAGCAGATGCATTGAAACTATTGCGTCAAAAGTCTTGAGCCACCCTACAAAGGTGAGTTTGTCACATAGTCATTCTAGAAGGATGAGGGATGACATATCTTGCAATGATACAGAAAGTTTGAGAATCAAATCAGGAAGTAAAGGATGGTGGGCTGAAGATTTAGCAGAACTTAGCATAGACCTTTATTGGAGAACAATGATAGCAATCAAATCTGGTGGCAAGGTTCCTTCAAATCTCATTGGTGATGCATTGAAAATTTATGCATCTAGATGGTTACCAAATATTTCCAAGAACGGAAATAGAAAGAAGAATGTTTCGCTAGCCGAGTCTGAGTCTAACTCAGACTCGGCCAGCGAAATAACTTCAAAGCATAGGCTTCTATTGGAGTCAATAGTAAGCTTGCTTCCAACAGAAAAAGGCGCTGTTTCTTGTAGTTTTCTTTTGAAACTATTGAAAGCATCCAATATTCTCAATGCTTCTTCATCTTCAAAGATGGAATTAGCAAGAAGGGTAGGACTTCAATTGGAGGAAGCCACAGTGAATGATCTATTAATACCTTCACTTTCTTATACAAATGATACAGTCTATGATGTTGAGTTGGTGATGACTATATTGGAACAGTTCATGTTACAAGGACAAAGTCCACCAACTAGCCCTCCGAGATCGTTGAAGACTTTTGAAAGAAGGCGATCACGTTCGGCTGAAAATATTAACTTTGAATTACAAGAGAGTAGGAGGTCCTCTTCAGCATCACATAGTTCAAAATTGAAAGTGGCAAAGCTTGTGGATAGGTACCTTCAGGAAGTTGCAAGAGATGTGAATTTTTCATTGTCAAAATTCATTGCTCTTGCTGATATTATACCAGAATTTGCAAGATATGATCATGATGATCTCTATAGAGCCATTGACATTTATCTCAAGGCTCATCCAGAACTCAACAAGAGTGAAAGGAAAAGACTATGTAGAATTCTTGACTGCAAAAAGCTCTCAATGGAAGCGTGCATGCATGCGGCGCAAAATGAGCTACTCCCTTTAAGAGTTGTTGTTCAAGTTCTGTTCTTTGAGCAAGCTAGAGCAGCATCATCTGGTGGCAAAGTAACAGAATTACAAAGCAACATCAAAGCATTGCTTACAACACATGGTATTGATCCATCGAAACATACAACAACGCAATTAAGCTCTACTACAAGTATTCAAGGTGAGGATAATTGGAGTGTTTCTGGTTTCAAGTCACCAAAATCAAAGTCTTCAACTCTAAGAATGAAGCTTGCTGAGGATCATGATGAATTTGATGAAAATGGTTTGGTAAATGATGATGGAATTGGAAGAAACTCTAAATTTAAGTCCATTTGTGCTCTTCCTACACAACCTAAAAAATGCTTAGTAAATTGTGGTCTACTAATAGAACTGCTACTGAAAAAAATTGAATATGAATTAAGTTATGTTAGTTTTTTCATGTTTGTAAGGGTAATTTAG
Protein sequence

>Msa0762310

MKFMKLGSRPDTFYTSEAIRTISSEVSSDLIIQVRGSRYLLHKFPLLSKCLCLQRLCSESPPDSSHHQIVQLPDFPGGVEAFELCAKFCYGITITLSPYNIVAARCAAEYLQMTEEVEKGNLIHKLEVFFNSCILHGWKDIIVSLQTTKALHLWSEDLGITSRCIETIASKVLSHPTKVSLSHSHSRRMRDDISCNDTESLRIKSGSKGWWAEDLAELSIDLYWRTMIAIKSGGKVPSNLIGDALKIYASRWLPNISKNGNRKKNVSLAESESNSDSASEITSKHRLLLESIVSLLPTEKGAVSCSFLLKLLKASNILNASSSSKMELARRVGLQLEEATVNDLLIPSLSYTNDTVYDVELVMTILEQFMLQGQSPPTSPPRSLKTFERRRSRSAENINFELQESRRSSSASHSSKLKVAKLVDRYLQEVARDVNFSLSKFIALADIIPEFARYDHDDLYRAIDIYLKAHPELNKSERKRLCRILDCKKLSMEACMHAAQNELLPLRVVVQVLFFEQARAASSGGKVTELQSNIKALLTTHGIDPSKHTTTQLSSTTSIQGEDNWSVSGFKSPKSKSSTLRMKLAEDHDEFDENGLVNDDGIGRNSKFKSICALPTQPKKCLVNCGLLIELLLKKIEYELSYVSFFMFVRVI*