AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0773320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773320 MtrunA17_Chr6g0454541 71.528 144 13 2 80 195 166 309 9.73e-44 152
Msa0773320 MtrunA17_Chr6g0454541 88.750 80 9 0 1 80 1 80 2.29e-34 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773320 AT5G15510.2 38.514 148 69 3 65 193 170 314 9.73e-17 78.2
Msa0773320 AT5G15510.4 38.514 148 69 3 65 193 170 314 1.18e-16 77.8
Msa0773320 AT5G15510.3 38.514 148 69 3 65 193 170 314 1.18e-16 77.8
Msa0773320 AT5G15510.1 38.514 148 69 3 65 193 170 314 1.45e-16 77.4
Msa0773320 AT3G01015.1 30.175 285 83 12 12 193 33 304 4.19e-15 73.2

Find 86 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAGTATTTGCAAATTT+TGG 0.123018 5_2:+48195415 Msa0773320:three_prime_UTR
AGAGGTTACTTAGTATAAAA+AGG 0.150829 5_2:+48192693 Msa0773320:CDS
GAACTATCTGAAGATGTTTC+TGG 0.200215 5_2:+48192794 Msa0773320:CDS
TTTAATCAATTGTATCAATT+TGG 0.219478 5_2:+48195445 Msa0773320:three_prime_UTR
AGGAATGGTGTACCTGAAAA+TGG 0.228755 5_2:+48192641 Msa0773320:CDS
TAAGGCATTGGCTGCGCTTT+AGG 0.259331 5_2:-48194857 None:intergenic
AAATTGATACAATTGATTAA+AGG 0.265677 5_2:-48195443 None:intergenic
ATGTGAAACTTTGGTTCTTT+TGG 0.278413 5_2:-48195268 None:intergenic
TTTCGATTAGAAAACTCATT+AGG 0.285358 5_2:-48192416 None:intergenic
TAAAGCACTTGATCTTCTTA+TGG 0.309995 5_2:-48195297 None:intergenic
AGAAATATTCAACTTGTATA+TGG 0.314202 5_2:+48195388 Msa0773320:three_prime_UTR
ATGAATGGTCTGTCAAAATA+AGG 0.316056 5_2:-48194875 None:intergenic
AGTTCATCTTCATCCTTAAC+AGG 0.322831 5_2:-48192776 None:intergenic
GGGTTATAGTTACATCTTCT+TGG 0.327723 5_2:+48192828 Msa0773320:CDS
TCCAAGGGTTTCTTCTTCTC+TGG 0.334067 5_2:-48192377 None:intergenic
AGAGAATGAGAAGAAGAATA+AGG 0.338941 5_2:+48192742 Msa0773320:CDS
TCACATAAGCAAATAAATTC+TGG 0.341016 5_2:-48195363 None:intergenic
TTCGATTAGAAAACTCATTA+GGG 0.343147 5_2:-48192415 None:intergenic
CATGGAAGACCTTGTGCAAT+AGG 0.361306 5_2:-48193585 None:intergenic
CTATGAAGCTTCACATCAAT+AGG 0.379023 5_2:-48194449 None:intergenic
TAGACATAAGCAGATGGATT+GGG 0.395938 5_2:+48196016 Msa0773320:three_prime_UTR
TTGGAATCATCTAGTACTAT+TGG 0.398723 5_2:+48193319 Msa0773320:CDS
AGAACAACAGAAAAGGGAAT+TGG 0.400583 5_2:+48194617 Msa0773320:three_prime_UTR
GGTTATAGTTACATCTTCTT+GGG 0.411476 5_2:+48192829 Msa0773320:CDS
CATTACCTCGTTGGAATGAA+TGG 0.424164 5_2:-48194890 None:intergenic
ATCTGAAGATGTTTCTGGAT+TGG 0.428685 5_2:+48192799 Msa0773320:CDS
TCTTCTTGGGATTGTGGAAG+TGG 0.435767 5_2:+48192842 Msa0773320:CDS
TGAGATGAGATGATATTAGA+AGG 0.438547 5_2:-48196107 None:intergenic
GGTGATGAAACAACCTGTTA+AGG 0.440822 5_2:+48192763 Msa0773320:CDS
TCGCGCGGAATTTGATCATC+AGG 0.446879 5_2:+48194490 Msa0773320:three_prime_UTR
TTGTGTGCAGTGTGTGTAGC+AGG 0.449144 5_2:+48192996 Msa0773320:intron
ACCTCAGTCCTTAGCTTGAA+AGG 0.454283 5_2:-48193389 None:intergenic
AGGAAGAGATTAGGAAATTG+AGG 0.454828 5_2:+48194822 Msa0773320:three_prime_UTR
GGGATTGTGGAAGTGGAAGA+AGG 0.460575 5_2:+48192849 Msa0773320:CDS
TGGCTGAAGAGGAAGAGATT+AGG 0.463010 5_2:+48194813 Msa0773320:three_prime_UTR
GGAATTGGAAATACAACAAA+AGG 0.463028 5_2:+48194632 Msa0773320:three_prime_UTR
AATTCGTCAGAGAAAGTTTG+TGG 0.463296 5_2:+48192511 Msa0773320:CDS
CAATTGTATCAATTTGGTTG+TGG 0.464497 5_2:+48195451 Msa0773320:three_prime_UTR
ATGTTTCTGGATTGGATCAA+AGG 0.473482 5_2:+48192807 Msa0773320:CDS
TTTGATAGAACAACAGAAAA+GGG 0.474688 5_2:+48194611 Msa0773320:three_prime_UTR
AGCAGATGGATTGGGGCAAA+AGG 0.476235 5_2:+48196024 Msa0773320:three_prime_UTR
GTTTGATAGAACAACAGAAA+AGG 0.481566 5_2:+48194610 Msa0773320:three_prime_UTR
TCCCTTACAGTTGGCTGAAG+AGG 0.485828 5_2:+48194802 Msa0773320:intron
GTTACATCTTCTTGGGATTG+TGG 0.485868 5_2:+48192836 Msa0773320:CDS
TATCAATTTGGTTGTGGAGT+AGG 0.495132 5_2:+48195457 Msa0773320:three_prime_UTR
GGAATGGTGTACCTGAAAAT+GGG 0.496291 5_2:+48192642 Msa0773320:CDS
GAAGCTTCATAGTGATTTGA+GGG 0.498697 5_2:+48194460 Msa0773320:three_prime_UTR
GTTCAAATTCTCAATTTCCA+AGG 0.500670 5_2:-48192393 None:intergenic
GTACTATTGGAGGAAGGAGG+TGG 0.500694 5_2:+48193332 Msa0773320:CDS
TGTTTCTGGATTGGATCAAA+GGG 0.501809 5_2:+48192808 Msa0773320:CDS
AGAAGAACAGTTGATGAAGA+AGG 0.503039 5_2:+48193524 Msa0773320:three_prime_UTR
TGAAGCTTCATAGTGATTTG+AGG 0.503817 5_2:+48194459 Msa0773320:three_prime_UTR
TTCAAATTCTCAATTTCCAA+GGG 0.511479 5_2:-48192392 None:intergenic
TTGATCAATCATTACCTCGT+TGG 0.518913 5_2:-48194899 None:intergenic
CTCTGACGAATTCTCTTCTG+CGG 0.520745 5_2:-48192500 None:intergenic
CTAGACATAAGCAGATGGAT+TGG 0.521537 5_2:+48196015 Msa0773320:three_prime_UTR
AAATGGGAAAGTGATGCATT+TGG 0.528141 5_2:+48192658 Msa0773320:CDS
GTCAGAAACCTTTCAAGCTA+AGG 0.529285 5_2:+48193381 Msa0773320:exon
TGGTGAGTTATGTGAAACTT+TGG 0.533377 5_2:-48195277 None:intergenic
TGATCAAAGTCCTAAGAAAA+CGG 0.535593 5_2:+48192553 Msa0773320:CDS
GAAAGAGAAGAATGAGGACG+AGG 0.540743 5_2:+48192718 Msa0773320:CDS
AGAGATTAGGAAATTGAGGA+AGG 0.551486 5_2:+48194826 Msa0773320:three_prime_UTR
CTATTGCACAAGGTCTTCCA+TGG 0.553915 5_2:+48193586 Msa0773320:three_prime_UTR
TTATTCGATGATTTCTGAAG+TGG 0.555085 5_2:-48192440 None:intergenic
ATTTGGTTGTGGAGTAGGTG+TGG 0.558070 5_2:+48195462 Msa0773320:three_prime_UTR
ACCAGAGAAGAAGAAACCCT+TGG 0.560006 5_2:+48192376 Msa0773320:CDS
TATTCGATGATTTCTGAAGT+GGG 0.560596 5_2:-48192439 None:intergenic
TTTGTGCTTTCTTTCACAGG+AGG 0.566913 5_2:-48194425 None:intergenic
GAATCATCTAGTACTATTGG+AGG 0.568710 5_2:+48193322 Msa0773320:CDS
TCAGGTTCATCAATAGTCCA+TGG 0.571822 5_2:-48193603 None:intergenic
AAGGGAGAAAGAGAAGAATG+AGG 0.575728 5_2:+48192712 Msa0773320:CDS
GGTCTGTCAAAATAAGGCAT+TGG 0.582294 5_2:-48194869 None:intergenic
TACTGCTAGACATAAGCAGA+TGG 0.591922 5_2:+48196010 Msa0773320:three_prime_UTR
AGACATAAGCAGATGGATTG+GGG 0.609461 5_2:+48196017 Msa0773320:three_prime_UTR
CATCTAGTACTATTGGAGGA+AGG 0.619078 5_2:+48193326 Msa0773320:CDS
AAATGTGGAGAGAATAGTGA+TGG 0.619254 5_2:+48192584 Msa0773320:CDS
GAAAGTGATGCATTTGGTGA+AGG 0.626466 5_2:+48192664 Msa0773320:CDS
GCAATGGAAGAAACAAGGAA+TGG 0.646394 5_2:+48192626 Msa0773320:intron
GCGAAGCAATGGAAGAAACA+AGG 0.647299 5_2:+48192621 Msa0773320:intron
CTAGTACTATTGGAGGAAGG+AGG 0.649542 5_2:+48193329 Msa0773320:CDS
TTTGAGGGCAATTGATCGCG+CGG 0.653049 5_2:+48194475 Msa0773320:three_prime_UTR
ATGGACTATTGATGAACCTG+AGG 0.663121 5_2:+48193605 Msa0773320:three_prime_UTR
ACCTTTCAAGCTAAGGACTG+AGG 0.672965 5_2:+48193388 Msa0773320:three_prime_UTR
ATGAGAATACCTATTGCACA+AGG 0.690231 5_2:+48193576 Msa0773320:three_prime_UTR
ATAATGCTATTGTAGCAAAG+GGG 0.728973 5_2:+48193492 Msa0773320:intron
ACAGACCATTCATTCCAACG+AGG 0.804126 5_2:+48194885 Msa0773320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATTACATTTATACT+TGG - chr5_2:48193719-48193738 None:intergenic 10.0%
!!! AGAGTTTTTTTTTTTTTTTT+TGG + chr5_2:48195659-48195678 Msa0773320:intron 10.0%
!!! TAATTGATGATACAAATAAT+TGG + chr5_2:48193411-48193430 Msa0773320:intron 15.0%
!! TTAAAAATTCTTTACATTGA+CGG + chr5_2:48193979-48193998 Msa0773320:intron 15.0%
!!! TTGTCTCATTATTTATATTT+TGG + chr5_2:48195216-48195235 Msa0773320:intron 15.0%
!!! TTATTTATATTTTGGTTTGA+AGG + chr5_2:48195224-48195243 Msa0773320:intron 15.0%
!! AAATTGATACAATTGATTAA+AGG - chr5_2:48195446-48195465 None:intergenic 15.0%
!! TTTAATCAATTGTATCAATT+TGG + chr5_2:48195445-48195464 Msa0773320:three_prime_UTR 15.0%
!!! TTTTTTTTTCTTCTTTGAAA+CGG - chr5_2:48195961-48195980 None:intergenic 15.0%
!!! TCATTTTTGCAAAATGTTAT+CGG + chr5_2:48193182-48193201 Msa0773320:intron 20.0%
!! ACTATAATACTAATGAAGAT+TGG + chr5_2:48193243-48193262 Msa0773320:intron 20.0%
!! TTAGTGATTCACTATTAATA+AGG + chr5_2:48193266-48193285 Msa0773320:intron 20.0%
!!! AGTTTCTTCAAATTTTTTAG+TGG + chr5_2:48193447-48193466 Msa0773320:intron 20.0%
!!! AAATTTTTTAGTGGCTATAT+TGG + chr5_2:48193456-48193475 Msa0773320:intron 20.0%
!!! GTAATTTTTTAATCAAGCAT+AGG + chr5_2:48193728-48193747 Msa0773320:intron 20.0%
!! AAAACTTTCTGTTTATTTGA+TGG + chr5_2:48193938-48193957 Msa0773320:intron 20.0%
!! CAAATTTATCATAATTCTGT+TGG + chr5_2:48194344-48194363 Msa0773320:intron 20.0%
!!! ATTTTAGATAAAGGAAAACA+TGG - chr5_2:48194764-48194783 None:intergenic 20.0%
!!! TTTATCTAAAATGTTGAAGT+CGG + chr5_2:48194772-48194791 Msa0773320:intron 20.0%
!! CAAAAAGAGACAAAAAATAA+TGG - chr5_2:48195199-48195218 None:intergenic 20.0%
!! AGAAATATTCAACTTGTATA+TGG + chr5_2:48195388-48195407 Msa0773320:three_prime_UTR 20.0%
!!! TTTTTTTTTTTTTTGGCTTC+TGG + chr5_2:48195666-48195685 Msa0773320:intron 20.0%
! TTCAAATTCTCAATTTCCAA+GGG - chr5_2:48192395-48192414 None:intergenic 25.0%
! TTCGATTAGAAAACTCATTA+GGG - chr5_2:48192418-48192437 None:intergenic 25.0%
! TTTCGATTAGAAAACTCATT+AGG - chr5_2:48192419-48192438 None:intergenic 25.0%
! AGAGGTTACTTAGTATAAAA+AGG + chr5_2:48192693-48192712 Msa0773320:CDS 25.0%
! GAGGTTACTTAGTATAAAAA+GGG + chr5_2:48192694-48192713 Msa0773320:CDS 25.0%
! ACATGTGATGTAATTAACAT+TGG + chr5_2:48193153-48193172 Msa0773320:intron 25.0%
! CATGTGATGTAATTAACATT+GGG + chr5_2:48193154-48193173 Msa0773320:intron 25.0%
! TATAATGCTATTGTAGCAAA+GGG + chr5_2:48193491-48193510 Msa0773320:intron 25.0%
! GAAAATTTAAATTGAACCAC+CGG - chr5_2:48193833-48193852 None:intergenic 25.0%
! ATGCTAGGATATTAGATATA+AGG - chr5_2:48194084-48194103 None:intergenic 25.0%
! ATAGCAATTCTCAATTAGAA+CGG + chr5_2:48194118-48194137 Msa0773320:intron 25.0%
! TCATGATGTGTAAAACTAAA+GGG - chr5_2:48194542-48194561 None:intergenic 25.0%
! TTCATGATGTGTAAAACTAA+AGG - chr5_2:48194543-48194562 None:intergenic 25.0%
! TTTGATAGAACAACAGAAAA+GGG + chr5_2:48194611-48194630 Msa0773320:three_prime_UTR 25.0%
!! GACTTCAACATTTTAGATAA+AGG - chr5_2:48194773-48194792 None:intergenic 25.0%
!!! GTATGTTTTTAACAGTTTCT+TGG + chr5_2:48194961-48194980 Msa0773320:intron 25.0%
!!! TTTAAAGTTGATTTGACGAA+AGG + chr5_2:48195084-48195103 Msa0773320:intron 25.0%
!!! GTCATTGATTTGAAATCAAT+TGG - chr5_2:48195121-48195140 None:intergenic 25.0%
! ATACACACTTTAATTGATCT+CGG - chr5_2:48195143-48195162 None:intergenic 25.0%
! TCACATAAGCAAATAAATTC+TGG - chr5_2:48195366-48195385 None:intergenic 25.0%
!! GCAATAGTATTTGCAAATTT+TGG + chr5_2:48195415-48195434 Msa0773320:three_prime_UTR 25.0%
!!! GAAATGAACTCACTTTTTTT+TGG + chr5_2:48196067-48196086 Msa0773320:three_prime_UTR 25.0%
GTTCAAATTCTCAATTTCCA+AGG - chr5_2:48192396-48192415 None:intergenic 30.0%
!! TATTCGATGATTTCTGAAGT+GGG - chr5_2:48192442-48192461 None:intergenic 30.0%
! TTATTCGATGATTTCTGAAG+TGG - chr5_2:48192443-48192462 None:intergenic 30.0%
TGATCAAAGTCCTAAGAAAA+CGG + chr5_2:48192553-48192572 Msa0773320:CDS 30.0%
! TTACACAAAACCGTTTTCTT+AGG - chr5_2:48192566-48192585 None:intergenic 30.0%
!! AAAACGGTTTTGTGTAAATG+TGG + chr5_2:48192569-48192588 Msa0773320:CDS 30.0%
AGAGAATGAGAAGAAGAATA+AGG + chr5_2:48192742-48192761 Msa0773320:CDS 30.0%
GGTTATAGTTACATCTTCTT+GGG + chr5_2:48192829-48192848 Msa0773320:CDS 30.0%
!!! TTTCTGTGTTTTTTAGAGCT+TGG + chr5_2:48193300-48193319 Msa0773320:intron 30.0%
TTGGAATCATCTAGTACTAT+TGG + chr5_2:48193319-48193338 Msa0773320:CDS 30.0%
CTATAATGCTATTGTAGCAA+AGG + chr5_2:48193490-48193509 Msa0773320:intron 30.0%
ATAATGCTATTGTAGCAAAG+GGG + chr5_2:48193492-48193511 Msa0773320:intron 30.0%
AGCATATACACTTCAAACTA+AGG - chr5_2:48193654-48193673 None:intergenic 30.0%
TAACAACTCACAGTCAAATT+TGG + chr5_2:48193677-48193696 Msa0773320:intron 30.0%
TCGTAATTGCATTACATGAT+GGG - chr5_2:48193797-48193816 None:intergenic 30.0%
ATCGTAATTGCATTACATGA+TGG - chr5_2:48193798-48193817 None:intergenic 30.0%
TTACGATTATAGCTTATGTC+CGG + chr5_2:48193811-48193830 Msa0773320:intron 30.0%
! TTTAAATTTTCACGCACTGT+CGG + chr5_2:48193842-48193861 Msa0773320:intron 30.0%
GAATTAAATCTGTGAGTAGT+TGG + chr5_2:48194009-48194028 Msa0773320:intron 30.0%
TCTAATATCCTAGCATAACA+TGG + chr5_2:48194088-48194107 Msa0773320:intron 30.0%
TGTACTAAATTCAGTTTCAG+TGG + chr5_2:48194252-48194271 Msa0773320:intron 30.0%
AATTCAGTTTCAGTGGAATT+AGG + chr5_2:48194259-48194278 Msa0773320:intron 30.0%
CATGATGTGTAAAACTAAAG+GGG - chr5_2:48194541-48194560 None:intergenic 30.0%
TGATGAATGCTTAACTGTTT+AGG + chr5_2:48194572-48194591 Msa0773320:intron 30.0%
GTTTGATAGAACAACAGAAA+AGG + chr5_2:48194610-48194629 Msa0773320:three_prime_UTR 30.0%
GGAATTGGAAATACAACAAA+AGG + chr5_2:48194632-48194651 Msa0773320:three_prime_UTR 30.0%
! TTGATTTGAGATGTGAAAGT+TGG - chr5_2:48194669-48194688 None:intergenic 30.0%
AAACATGGCAACTCAAAATT+AGG - chr5_2:48194749-48194768 None:intergenic 30.0%
ATGAATGGTCTGTCAAAATA+AGG - chr5_2:48194878-48194897 None:intergenic 30.0%
! TGGCATATCATTTTGTGATT+CGG + chr5_2:48194981-48195000 Msa0773320:intron 30.0%
TCATTCGATCTCAAAATCAA+TGG + chr5_2:48195052-48195071 Msa0773320:intron 30.0%
! ATTTTGCACTGTTGTGAATT+CGG + chr5_2:48195171-48195190 Msa0773320:intron 30.0%
!!! TTTGGTTCTTTTGGTATAGT+TGG - chr5_2:48195262-48195281 None:intergenic 30.0%
!!! ATGTGAAACTTTGGTTCTTT+TGG - chr5_2:48195271-48195290 None:intergenic 30.0%
! TAAAGCACTTGATCTTCTTA+TGG - chr5_2:48195300-48195319 None:intergenic 30.0%
CAATTGTATCAATTTGGTTG+TGG + chr5_2:48195451-48195470 Msa0773320:three_prime_UTR 30.0%
!!! GAGTTTTTTGTTGCTGTTTT+TGG + chr5_2:48195802-48195821 Msa0773320:intron 30.0%
TGAGATGAGATGATATTAGA+AGG - chr5_2:48196110-48196129 None:intergenic 30.0%
AATTCGTCAGAGAAAGTTTG+TGG + chr5_2:48192511-48192530 Msa0773320:CDS 35.0%
AAATGTGGAGAGAATAGTGA+TGG + chr5_2:48192584-48192603 Msa0773320:CDS 35.0%
AAATGGGAAAGTGATGCATT+TGG + chr5_2:48192658-48192677 Msa0773320:CDS 35.0%
!! ATTTGGTGAAGGATTTTGAG+AGG + chr5_2:48192675-48192694 Msa0773320:CDS 35.0%
AGTTCATCTTCATCCTTAAC+AGG - chr5_2:48192779-48192798 None:intergenic 35.0%
GAACTATCTGAAGATGTTTC+TGG + chr5_2:48192794-48192813 Msa0773320:CDS 35.0%
!! ATCTGAAGATGTTTCTGGAT+TGG + chr5_2:48192799-48192818 Msa0773320:CDS 35.0%
!! ATGTTTCTGGATTGGATCAA+AGG + chr5_2:48192807-48192826 Msa0773320:CDS 35.0%
!! TGTTTCTGGATTGGATCAAA+GGG + chr5_2:48192808-48192827 Msa0773320:CDS 35.0%
GGGTTATAGTTACATCTTCT+TGG + chr5_2:48192828-48192847 Msa0773320:CDS 35.0%
CATGCCTTAATCACAAATGT+CGG - chr5_2:48193115-48193134 None:intergenic 35.0%
ATTTGTGATTAAGGCATGTC+CGG + chr5_2:48193117-48193136 Msa0773320:intron 35.0%
GAATCATCTAGTACTATTGG+AGG + chr5_2:48193322-48193341 Msa0773320:CDS 35.0%
!! AGAAGAACAGTTGATGAAGA+AGG + chr5_2:48193524-48193543 Msa0773320:three_prime_UTR 35.0%
ATGAGAATACCTATTGCACA+AGG + chr5_2:48193576-48193595 Msa0773320:three_prime_UTR 35.0%
! TTAAATTTTCACGCACTGTC+GGG + chr5_2:48193843-48193862 Msa0773320:intron 35.0%
TAACGCAGCAACATCATTAA+GGG - chr5_2:48194185-48194204 None:intergenic 35.0%
TTAACGCAGCAACATCATTA+AGG - chr5_2:48194186-48194205 None:intergenic 35.0%
AGACTCAAGAGGATGTTTAT+TGG - chr5_2:48194212-48194231 None:intergenic 35.0%
AGTACATATCAGCTGACATA+TGG - chr5_2:48194238-48194257 None:intergenic 35.0%
CAGAAGAATGCTTAGACAAA+TGG - chr5_2:48194387-48194406 None:intergenic 35.0%
CTATGAAGCTTCACATCAAT+AGG - chr5_2:48194452-48194471 None:intergenic 35.0%
TGAAGCTTCATAGTGATTTG+AGG + chr5_2:48194459-48194478 Msa0773320:three_prime_UTR 35.0%
GAAGCTTCATAGTGATTTGA+GGG + chr5_2:48194460-48194479 Msa0773320:three_prime_UTR 35.0%
TGAATGCTTAACTGTTTAGG+TGG + chr5_2:48194575-48194594 Msa0773320:intron 35.0%
AGAACAACAGAAAAGGGAAT+TGG + chr5_2:48194617-48194636 Msa0773320:three_prime_UTR 35.0%
!! GATGTGAAAGTTGGTAAACA+TGG - chr5_2:48194660-48194679 None:intergenic 35.0%
GATGTGAAAGTTGGAAAACA+TGG - chr5_2:48194712-48194731 None:intergenic 35.0%
! CTGATTTGAGATGTGAAAGT+TGG - chr5_2:48194721-48194740 None:intergenic 35.0%
GGTATATATTCCCTTACAGT+TGG + chr5_2:48194793-48194812 Msa0773320:intron 35.0%
AGGAAGAGATTAGGAAATTG+AGG + chr5_2:48194822-48194841 Msa0773320:three_prime_UTR 35.0%
AGAGATTAGGAAATTGAGGA+AGG + chr5_2:48194826-48194845 Msa0773320:three_prime_UTR 35.0%
TTGATCAATCATTACCTCGT+TGG - chr5_2:48194902-48194921 None:intergenic 35.0%
TGGTGAGTTATGTGAAACTT+TGG - chr5_2:48195280-48195299 None:intergenic 35.0%
TATCAATTTGGTTGTGGAGT+AGG + chr5_2:48195457-48195476 Msa0773320:three_prime_UTR 35.0%
! TGGATTCTGTCTTTTCTGTT+TGG + chr5_2:48195482-48195501 Msa0773320:intron 35.0%
! TGGTTTGAGCAGATAGTTTT+TGG + chr5_2:48195502-48195521 Msa0773320:intron 35.0%
!! TTGACTGATTTGCTGTGTTA+GGG + chr5_2:48195616-48195635 Msa0773320:intron 35.0%
ATATAAAACACAGCAGCTGA+TGG - chr5_2:48195745-48195764 None:intergenic 35.0%
AACCAAGAGACTGAAAAATG+AGG - chr5_2:48195773-48195792 None:intergenic 35.0%
!! ATTTTGGGTGTGTTTTCTTG+AGG + chr5_2:48195849-48195868 Msa0773320:intron 35.0%
! GGCAAATTTTATTCACGTCA+AGG - chr5_2:48195940-48195959 None:intergenic 35.0%
! TAGACATAAGCAGATGGATT+GGG + chr5_2:48196016-48196035 Msa0773320:three_prime_UTR 35.0%
TAAGTGTGTTTGCGAAGCAA+TGG + chr5_2:48192610-48192629 Msa0773320:intron 40.0%
GCAATGGAAGAAACAAGGAA+TGG + chr5_2:48192626-48192645 Msa0773320:intron 40.0%
AGGAATGGTGTACCTGAAAA+TGG + chr5_2:48192641-48192660 Msa0773320:CDS 40.0%
GGAATGGTGTACCTGAAAAT+GGG + chr5_2:48192642-48192661 Msa0773320:CDS 40.0%
! TGCATCACTTTCCCATTTTC+AGG - chr5_2:48192656-48192675 None:intergenic 40.0%
! GAAAGTGATGCATTTGGTGA+AGG + chr5_2:48192664-48192683 Msa0773320:CDS 40.0%
AAGGGAGAAAGAGAAGAATG+AGG + chr5_2:48192712-48192731 Msa0773320:CDS 40.0%
GGTGATGAAACAACCTGTTA+AGG + chr5_2:48192763-48192782 Msa0773320:CDS 40.0%
GTTACATCTTCTTGGGATTG+TGG + chr5_2:48192836-48192855 Msa0773320:CDS 40.0%
CCTTAATCACAAATGTCGGT+CGG - chr5_2:48193111-48193130 None:intergenic 40.0%
TTACATCACATGTGTCGTGT+CGG - chr5_2:48193146-48193165 None:intergenic 40.0%
GTGTTAGTGTTTGTGACATG+CGG + chr5_2:48193218-48193237 Msa0773320:intron 40.0%
CATCTAGTACTATTGGAGGA+AGG + chr5_2:48193326-48193345 Msa0773320:CDS 40.0%
GTCAGAAACCTTTCAAGCTA+AGG + chr5_2:48193381-48193400 Msa0773320:exon 40.0%
!! TGGCTATATTGGTGATGCAT+TGG + chr5_2:48193467-48193486 Msa0773320:intron 40.0%
TCAGGTTCATCAATAGTCCA+TGG - chr5_2:48193606-48193625 None:intergenic 40.0%
! ATGGACTATTGATGAACCTG+AGG + chr5_2:48193605-48193624 Msa0773320:three_prime_UTR 40.0%
CTATTGATGAACCTGAGGTA+AGG + chr5_2:48193610-48193629 Msa0773320:intron 40.0%
! GCTAATGGACAAGCATTAGT+AGG - chr5_2:48193764-48193783 None:intergenic 40.0%
! TTACATGATGGGACAATCAG+AGG - chr5_2:48193786-48193805 None:intergenic 40.0%
! TAAATTTTCACGCACTGTCG+GGG + chr5_2:48193844-48193863 Msa0773320:intron 40.0%
TATCCTAGCATAACATGGCA+AGG + chr5_2:48194093-48194112 Msa0773320:intron 40.0%
TCAATTAGAACGGTACGTTG+TGG + chr5_2:48194128-48194147 Msa0773320:intron 40.0%
ACATATGGTCAAGACTCAAG+AGG - chr5_2:48194223-48194242 None:intergenic 40.0%
!! ACGAGGTATTGTGAGCAAAA+AGG - chr5_2:48194288-48194307 None:intergenic 40.0%
! ACAAACACAGAAAGTACACG+AGG - chr5_2:48194305-48194324 None:intergenic 40.0%
TTTGTGCTTTCTTTCACAGG+AGG - chr5_2:48194428-48194447 None:intergenic 40.0%
!! GGTTTTGTGCTTTCTTTCAC+AGG - chr5_2:48194431-48194450 None:intergenic 40.0%
GGTCTGTCAAAATAAGGCAT+TGG - chr5_2:48194872-48194891 None:intergenic 40.0%
CATTACCTCGTTGGAATGAA+TGG - chr5_2:48194893-48194912 None:intergenic 40.0%
CATGACACAGTTATCCATCT+TGG + chr5_2:48195029-48195048 Msa0773320:intron 40.0%
TTGAGATCGAATGACCAAGA+TGG - chr5_2:48195046-48195065 None:intergenic 40.0%
GTAAATCTGGTGTGCTCTAT+TGG + chr5_2:48195573-48195592 Msa0773320:intron 40.0%
!! GTTGACTGATTTGCTGTGTT+AGG + chr5_2:48195615-48195634 Msa0773320:intron 40.0%
!! TGACTGATTTGCTGTGTTAG+GGG + chr5_2:48195617-48195636 Msa0773320:intron 40.0%
!! TGCCTCATTTTTCAGTCTCT+TGG + chr5_2:48195768-48195787 Msa0773320:intron 40.0%
TCAGTCTCTTGGTTAACTGT+AGG + chr5_2:48195779-48195798 Msa0773320:intron 40.0%
CAGTCTCTTGGTTAACTGTA+GGG + chr5_2:48195780-48195799 Msa0773320:intron 40.0%
GAAAGGGAGAAAACAAACAC+AGG - chr5_2:48195891-48195910 None:intergenic 40.0%
TACTGCTAGACATAAGCAGA+TGG + chr5_2:48196010-48196029 Msa0773320:three_prime_UTR 40.0%
CTAGACATAAGCAGATGGAT+TGG + chr5_2:48196015-48196034 Msa0773320:three_prime_UTR 40.0%
!! AGACATAAGCAGATGGATTG+GGG + chr5_2:48196017-48196036 Msa0773320:three_prime_UTR 40.0%
ACCAGAGAAGAAGAAACCCT+TGG + chr5_2:48192376-48192395 Msa0773320:CDS 45.0%
TCCAAGGGTTTCTTCTTCTC+TGG - chr5_2:48192380-48192399 None:intergenic 45.0%
CTCTGACGAATTCTCTTCTG+CGG - chr5_2:48192503-48192522 None:intergenic 45.0%
GCGAAGCAATGGAAGAAACA+AGG + chr5_2:48192621-48192640 Msa0773320:intron 45.0%
GAAAGAGAAGAATGAGGACG+AGG + chr5_2:48192718-48192737 Msa0773320:CDS 45.0%
TCTTCTTGGGATTGTGGAAG+TGG + chr5_2:48192842-48192861 Msa0773320:CDS 45.0%
! GTGTGTAGCAGGAACTTTTC+TGG + chr5_2:48193007-48193026 Msa0773320:CDS 45.0%
! TGTAGCAGGAACTTTTCTGG+TGG + chr5_2:48193010-48193029 Msa0773320:CDS 45.0%
CCGACCGACATTTGTGATTA+AGG + chr5_2:48193108-48193127 Msa0773320:intron 45.0%
CTAGTACTATTGGAGGAAGG+AGG + chr5_2:48193329-48193348 Msa0773320:CDS 45.0%
ACCTTTCAAGCTAAGGACTG+AGG + chr5_2:48193388-48193407 Msa0773320:three_prime_UTR 45.0%
! ACCTCAGTCCTTAGCTTGAA+AGG - chr5_2:48193392-48193411 None:intergenic 45.0%
CATGGAAGACCTTGTGCAAT+AGG - chr5_2:48193588-48193607 None:intergenic 45.0%
CTATTGCACAAGGTCTTCCA+TGG + chr5_2:48193586-48193605 Msa0773320:three_prime_UTR 45.0%
TGAGAAATCGACCTTACCTC+AGG - chr5_2:48193624-48193643 None:intergenic 45.0%
ATGGGACAATCAGAGGCTAA+TGG - chr5_2:48193779-48193798 None:intergenic 45.0%
CGATTATAGCTTATGTCCGG+TGG + chr5_2:48193814-48193833 Msa0773320:intron 45.0%
TCTCCTTGCCATGTTATGCT+AGG - chr5_2:48194099-48194118 None:intergenic 45.0%
TCCTCTTCAGCCAACTGTAA+GGG - chr5_2:48194806-48194825 None:intergenic 45.0%
TTCCTCTTCAGCCAACTGTA+AGG - chr5_2:48194807-48194826 None:intergenic 45.0%
TGGCTGAAGAGGAAGAGATT+AGG + chr5_2:48194813-48194832 Msa0773320:three_prime_UTR 45.0%
ACAGACCATTCATTCCAACG+AGG + chr5_2:48194885-48194904 Msa0773320:three_prime_UTR 45.0%
ATTTGGTTGTGGAGTAGGTG+TGG + chr5_2:48195462-48195481 Msa0773320:three_prime_UTR 45.0%
!! GACTGATTTGCTGTGTTAGG+GGG + chr5_2:48195618-48195637 Msa0773320:intron 45.0%
!! ACTGATTTGCTGTGTTAGGG+GGG + chr5_2:48195619-48195638 Msa0773320:intron 45.0%
CTGGCTGTTCTTTCTTGGTT+TGG + chr5_2:48195685-48195704 Msa0773320:intron 45.0%
!!! TGCTGTTTTTGGTCAGCTGT+CGG + chr5_2:48195813-48195832 Msa0773320:intron 45.0%
!! GGCCTTGTGACAGTGATTTT+GGG + chr5_2:48195834-48195853 Msa0773320:intron 45.0%
CACCCAAAATCACTGTCACA+AGG - chr5_2:48195839-48195858 None:intergenic 45.0%
! TTCTTGAGGTGTTCCGTGTT+AGG + chr5_2:48195863-48195882 Msa0773320:intron 45.0%
! TTGTTTTCTCCCTTTCTGCG+AGG + chr5_2:48195895-48195914 Msa0773320:intron 45.0%
! TGTTTTCTCCCTTTCTGCGA+GGG + chr5_2:48195896-48195915 Msa0773320:intron 45.0%
GGGATTGTGGAAGTGGAAGA+AGG + chr5_2:48192849-48192868 Msa0773320:CDS 50.0%
TTGTGTGCAGTGTGTGTAGC+AGG + chr5_2:48192996-48193015 Msa0773320:intron 50.0%
GTACTATTGGAGGAAGGAGG+TGG + chr5_2:48193332-48193351 Msa0773320:CDS 50.0%
TTTGAGGGCAATTGATCGCG+CGG + chr5_2:48194475-48194494 Msa0773320:three_prime_UTR 50.0%
TCGCGCGGAATTTGATCATC+AGG + chr5_2:48194490-48194509 Msa0773320:three_prime_UTR 50.0%
TCCCTTACAGTTGGCTGAAG+AGG + chr5_2:48194802-48194821 Msa0773320:intron 50.0%
!! TAAGGCATTGGCTGCGCTTT+AGG - chr5_2:48194860-48194879 None:intergenic 50.0%
! TTGTTTTCTTCCGTGCCTGC+TGG + chr5_2:48195529-48195548 Msa0773320:intron 50.0%
CAGATCAAAACCAGCAGGCA+CGG - chr5_2:48195542-48195561 None:intergenic 50.0%
CGCAACAGATCAAAACCAGC+AGG - chr5_2:48195547-48195566 None:intergenic 50.0%
!! GTTTTGATCTGTTGCGCCTG+TGG + chr5_2:48195551-48195570 Msa0773320:intron 50.0%
TGTTGCGCCTGTGGTAAATC+TGG + chr5_2:48195560-48195579 Msa0773320:intron 50.0%
GAGCACACCAGATTTACCAC+AGG - chr5_2:48195570-48195589 None:intergenic 50.0%
! TGGTGTGCTCTATTGGTGCT+GGG + chr5_2:48195580-48195599 Msa0773320:intron 50.0%
! GGCTTCTGGCTGTTCTTTCT+TGG + chr5_2:48195680-48195699 Msa0773320:intron 50.0%
!! CGGCCTTGTGACAGTGATTT+TGG + chr5_2:48195833-48195852 Msa0773320:intron 50.0%
ACAAACACAGGCGCCTAACA+CGG - chr5_2:48195879-48195898 None:intergenic 50.0%
!! AGCAGATGGATTGGGGCAAA+AGG + chr5_2:48196024-48196043 Msa0773320:three_prime_UTR 50.0%
GTCGGTCGGTACTATAGAGC+TGG - chr5_2:48193097-48193116 None:intergenic 55.0%
ACATGTGTCGTGTCGGACAC+CGG - chr5_2:48193139-48193158 None:intergenic 55.0%
CTGGTGTGCTCTATTGGTGC+TGG + chr5_2:48195579-48195598 Msa0773320:intron 55.0%
!! TGTGCTCTATTGGTGCTGGG+AGG + chr5_2:48195583-48195602 Msa0773320:intron 55.0%
!! ATTGGTGCTGGGAGGTGGTT+TGG + chr5_2:48195591-48195610 Msa0773320:intron 55.0%
!! ATTTGCTGTGTTAGGGGGGC+TGG + chr5_2:48195623-48195642 Msa0773320:intron 55.0%
TTCTCCCTTTCTGCGAGGGT+TGG + chr5_2:48195900-48195919 Msa0773320:intron 55.0%
CACACCAACCCTCGCAGAAA+GGG - chr5_2:48195907-48195926 None:intergenic 55.0%
ACACACCAACCCTCGCAGAA+AGG - chr5_2:48195908-48195927 None:intergenic 55.0%
!! GCTCTATTGGTGCTGGGAGG+TGG + chr5_2:48195586-48195605 Msa0773320:intron 60.0%
CTGTGTTAGGGGGGCTGGAA+TGG + chr5_2:48195628-48195647 Msa0773320:intron 60.0%
TAGGGGGGCTGGAATGGTAC+AGG + chr5_2:48195634-48195653 Msa0773320:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 48192371 48196131 48192371 ID=Msa0773320;Name=Msa0773320
chr5_2 mRNA 48192371 48196131 48192371 ID=Msa0773320-mRNA-1;Parent=Msa0773320;Name=Msa0773320-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0.88|0.7|1|0.66|0.7|10|793|197
chr5_2 exon 48192371 48192608 48192371 ID=Msa0773320-mRNA-1:exon:14435;Parent=Msa0773320-mRNA-1
chr5_2 exon 48192627 48192870 48192627 ID=Msa0773320-mRNA-1:exon:14436;Parent=Msa0773320-mRNA-1
chr5_2 exon 48193006 48193051 48193006 ID=Msa0773320-mRNA-1:exon:14437;Parent=Msa0773320-mRNA-1
chr5_2 exon 48193316 48193409 48193316 ID=Msa0773320-mRNA-1:exon:14438;Parent=Msa0773320-mRNA-1
chr5_2 exon 48193507 48193626 48193507 ID=Msa0773320-mRNA-1:exon:14439;Parent=Msa0773320-mRNA-1
chr5_2 exon 48194413 48194511 48194413 ID=Msa0773320-mRNA-1:exon:14440;Parent=Msa0773320-mRNA-1
chr5_2 exon 48194594 48194653 48194594 ID=Msa0773320-mRNA-1:exon:14441;Parent=Msa0773320-mRNA-1
chr5_2 exon 48194812 48194906 48194812 ID=Msa0773320-mRNA-1:exon:14442;Parent=Msa0773320-mRNA-1
chr5_2 exon 48195246 48195498 48195246 ID=Msa0773320-mRNA-1:exon:14443;Parent=Msa0773320-mRNA-1
chr5_2 exon 48195994 48196131 48195994 ID=Msa0773320-mRNA-1:exon:14444;Parent=Msa0773320-mRNA-1
chr5_2 CDS 48192371 48192608 48192371 ID=Msa0773320-mRNA-1:cds;Parent=Msa0773320-mRNA-1
chr5_2 CDS 48192627 48192870 48192627 ID=Msa0773320-mRNA-1:cds;Parent=Msa0773320-mRNA-1
chr5_2 CDS 48193006 48193051 48193006 ID=Msa0773320-mRNA-1:cds;Parent=Msa0773320-mRNA-1
chr5_2 CDS 48193316 48193381 48193316 ID=Msa0773320-mRNA-1:cds;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48193382 48193409 48193382 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48193507 48193626 48193507 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48194413 48194511 48194413 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48194594 48194653 48194594 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48194812 48194906 48194812 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48195246 48195498 48195246 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
chr5_2 three_prime_UTR 48195994 48196131 48195994 ID=Msa0773320-mRNA-1:three_prime_utr;Parent=Msa0773320-mRNA-1
Gene Sequence

>Msa0773320

ATGACACCAGAGAAGAAGAAACCCTTGGAAATTGAGAATTTGAACCCTAATGAGTTTTCTAATCGAAACCCACTTCAGAAATCATCGAATAAAGCTGAGAATAATTGTGTTAATGTTTCTGTTGTTTCGCCGCAGAAGAGAATTCGTCAGAGAAAGTTTGTGGTTGCAAAGAAGAAGAAGAATGATCAAAGTCCTAAGAAAACGGTTTTGTGTAAATGTGGAGAGAATAGTGATGGAACAATGGAAGAAACAAGGAATGGTGTACCTGAAAATGGGAAAGTGATGCATTTGGTGAAGGATTTTGAGAGGTTACTTAGTATAAAAAGGGAGAAAGAGAAGAATGAGGACGAGGAAGAGAATGAGAAGAAGAATAAGGTGATGAAACAACCTGTTAAGGATGAAGATGAACTATCTGAAGATGTTTCTGGATTGGATCAAAGGGTTATAGTTACATCTTCTTGGGATTGTGGAAGTGGAAGAAGTGTGTGTAGCAGGAACTTTTCTGGTGGTAGAAGAAGCAGAAGAAATAGCTTGGAATCATCTAGTACTATTGGAGGAAGGAGGTGGAAGAAGAAGCAGAAGCTAAGTCACTAG
Protein sequence

>Msa0773320

MTPEKKKPLEIENLNPNEFSNRNPLQKSSNKAENNCVNVSVVSPQKRIRQRKFVVAKKKKNDQSPKKTVLCKCGENSDGTMEETRNGVPENGKVMHLVKDFERLLSIKREKEKNEDEEENEKKNKVMKQPVKDEDELSEDVSGLDQRVIVTSSWDCGSGRSVCSRNFSGGRRSRRNSLESSSTIGGRRWKKKQKLSH*