Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0285450 | Msa0773530 | 0.809495 | 1.839475e-50 | -8.615850e-47 |
| Msa0518380 | Msa0773530 | 0.800615 | 1.326634e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0773530 | MtrunA17_Chr2g0325821 | 62.745 | 51 | 17 | 1 | 25 | 73 | 187 | 237 | 6.38e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAAAGCCATTCAAATTC+TGG | 0.199920 | 5_2:+48510263 | None:intergenic |
| CATCATTTCAAGTTGGTAAT+TGG | 0.201134 | 5_2:+48508639 | None:intergenic |
| CACAAAGCCATTCAAATTCT+GGG | 0.380519 | 5_2:+48510264 | None:intergenic |
| TCAAGTTCTTGCTTATCATC+TGG | 0.412579 | 5_2:+48508914 | None:intergenic |
| TTGAATGGCTTTGTGATAGT+AGG | 0.420299 | 5_2:-48510256 | Msa0773530:intron |
| TGATTTCGGTTGCATGTTTG+AGG | 0.434933 | 5_2:-48510324 | None:intergenic |
| TTTCAATTCAAAATAAAAGA+TGG | 0.445676 | 5_2:+48510298 | None:intergenic |
| CTGGGAACCCAGAATTTGAA+TGG | 0.470589 | 5_2:-48510271 | Msa0773530:CDS |
| GACGAGGAAATTGAAGCTGA+TGG | 0.566448 | 5_2:-48508664 | Msa0773530:CDS |
| AGCAACGCATCATTTCAAGT+TGG | 0.576254 | 5_2:+48508632 | None:intergenic |
| TGATAAGCAAGAACTTGATG+AGG | 0.625711 | 5_2:-48508909 | Msa0773530:CDS |
| GCAGATGAGGGAAGAAGACG+AGG | 0.653304 | 5_2:-48508680 | Msa0773530:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAACTACTTTTAAAAATTT+AGG | + | chr5_2:48509755-48509774 | None:intergenic | 10.0% |
| !! | AATTTATAAAGTACAATTTA+TGG | - | chr5_2:48509894-48509913 | Msa0773530:intron | 10.0% |
| !!! | TAAACTACTTTTAAAAATTT+AGG | + | chr5_2:48509755-48509774 | None:intergenic | 10.0% |
| !! | AATTTATAAAGTACAATTTA+TGG | - | chr5_2:48509894-48509913 | Msa0773530:intron | 10.0% |
| !! | TTTCAATTCAAAATAAAAGA+TGG | + | chr5_2:48508643-48508662 | None:intergenic | 15.0% |
| !!! | TTTTATACATGCTTAAAAAT+TGG | - | chr5_2:48509170-48509189 | Msa0773530:intron | 15.0% |
| !!! | TTTCTGAAATTATATATGAT+AGG | - | chr5_2:48509413-48509432 | Msa0773530:intron | 15.0% |
| !! | TTTCAATTCAAAATAAAAGA+TGG | + | chr5_2:48508643-48508662 | None:intergenic | 15.0% |
| !!! | TTTTATACATGCTTAAAAAT+TGG | - | chr5_2:48509170-48509189 | Msa0773530:intron | 15.0% |
| !!! | TTTCTGAAATTATATATGAT+AGG | - | chr5_2:48509413-48509432 | Msa0773530:intron | 15.0% |
| !!! | TATTTTGAATTGAAATATGC+TGG | - | chr5_2:48508648-48508667 | Msa0773530:CDS | 20.0% |
| !!! | ATTTTGAATTGAAATATGCT+GGG | - | chr5_2:48508649-48508668 | Msa0773530:CDS | 20.0% |
| !!! | TATGTTGATTTTTTGTTTCT+CGG | - | chr5_2:48508740-48508759 | Msa0773530:intron | 20.0% |
| !! | ATCAAATTCAAAGAAAGTTA+AGG | + | chr5_2:48509086-48509105 | None:intergenic | 20.0% |
| !! | AATAAATGTCTTGTAATTAC+AGG | - | chr5_2:48509864-48509883 | Msa0773530:intron | 20.0% |
| !! | ATTGCATCATATATTCAATA+AGG | + | chr5_2:48510083-48510102 | None:intergenic | 20.0% |
| !!! | TATTTTGAATTGAAATATGC+TGG | - | chr5_2:48508648-48508667 | Msa0773530:CDS | 20.0% |
| !!! | ATTTTGAATTGAAATATGCT+GGG | - | chr5_2:48508649-48508668 | Msa0773530:CDS | 20.0% |
| !!! | TATGTTGATTTTTTGTTTCT+CGG | - | chr5_2:48508740-48508759 | Msa0773530:intron | 20.0% |
| !! | ATCAAATTCAAAGAAAGTTA+AGG | + | chr5_2:48509086-48509105 | None:intergenic | 20.0% |
| !! | AATAAATGTCTTGTAATTAC+AGG | - | chr5_2:48509864-48509883 | Msa0773530:intron | 20.0% |
| !! | ATTGCATCATATATTCAATA+AGG | + | chr5_2:48510083-48510102 | None:intergenic | 20.0% |
| ! | GTAGTGTTTACATGATATTT+AGG | + | chr5_2:48509352-48509371 | None:intergenic | 25.0% |
| !!! | TTCTAGCATTGATTTATTTC+AGG | + | chr5_2:48509563-48509582 | None:intergenic | 25.0% |
| ! | AAACATGAAATTCTTGAGAT+TGG | - | chr5_2:48509591-48509610 | Msa0773530:intron | 25.0% |
| !! | AGTTCTTTTAACAACTTCAA+TGG | + | chr5_2:48509637-48509656 | None:intergenic | 25.0% |
| ! | ATTGAAGTTGTTAAAAGAAC+TGG | - | chr5_2:48509636-48509655 | Msa0773530:intron | 25.0% |
| !! | CATTTATTGATTAAGCATCT+AGG | + | chr5_2:48509852-48509871 | None:intergenic | 25.0% |
| !!! | ATTTCTAAAATTTGCAGATG+AGG | - | chr5_2:48510245-48510264 | Msa0773530:intron | 25.0% |
| !!! | TTTCTAAAATTTGCAGATGA+GGG | - | chr5_2:48510246-48510265 | Msa0773530:intron | 25.0% |
| ! | GTAGTGTTTACATGATATTT+AGG | + | chr5_2:48509352-48509371 | None:intergenic | 25.0% |
| !!! | TTCTAGCATTGATTTATTTC+AGG | + | chr5_2:48509563-48509582 | None:intergenic | 25.0% |
| ! | AAACATGAAATTCTTGAGAT+TGG | - | chr5_2:48509591-48509610 | Msa0773530:intron | 25.0% |
| !! | AGTTCTTTTAACAACTTCAA+TGG | + | chr5_2:48509637-48509656 | None:intergenic | 25.0% |
| ! | ATTGAAGTTGTTAAAAGAAC+TGG | - | chr5_2:48509636-48509655 | Msa0773530:intron | 25.0% |
| !! | CATTTATTGATTAAGCATCT+AGG | + | chr5_2:48509852-48509871 | None:intergenic | 25.0% |
| !!! | ATTTCTAAAATTTGCAGATG+AGG | - | chr5_2:48510245-48510264 | Msa0773530:intron | 25.0% |
| !!! | TTTCTAAAATTTGCAGATGA+GGG | - | chr5_2:48510246-48510265 | Msa0773530:intron | 25.0% |
| !!! | CTCGGTTGTTTTTTTTCATT+TGG | - | chr5_2:48508758-48508777 | Msa0773530:intron | 30.0% |
| ! | TTGTTTGAGTTTGTTTGTGT+TGG | - | chr5_2:48508789-48508808 | Msa0773530:intron | 30.0% |
| ! | TCATGTATGCATGTTTGTTT+TGG | - | chr5_2:48508860-48508879 | Msa0773530:intron | 30.0% |
| TCTCATTGTTACACTTCAAT+TGG | - | chr5_2:48508938-48508957 | Msa0773530:CDS | 30.0% | |
| CTCATTGTTACACTTCAATT+GGG | - | chr5_2:48508939-48508958 | Msa0773530:CDS | 30.0% | |
| TGATTTCTCTCTCTGTAATT+TGG | - | chr5_2:48509002-48509021 | Msa0773530:intron | 30.0% | |
| ATATGATAGGATGAAGATGA+AGG | - | chr5_2:48509426-48509445 | Msa0773530:intron | 30.0% | |
| AAGTACAATTTATGGAGTTG+TGG | - | chr5_2:48509902-48509921 | Msa0773530:intron | 30.0% | |
| !! | CATCATTTCAAGTTGGTAAT+TGG | + | chr5_2:48510302-48510321 | None:intergenic | 30.0% |
| !!! | CTCGGTTGTTTTTTTTCATT+TGG | - | chr5_2:48508758-48508777 | Msa0773530:intron | 30.0% |
| ! | TTGTTTGAGTTTGTTTGTGT+TGG | - | chr5_2:48508789-48508808 | Msa0773530:intron | 30.0% |
| ! | TCATGTATGCATGTTTGTTT+TGG | - | chr5_2:48508860-48508879 | Msa0773530:intron | 30.0% |
| TCTCATTGTTACACTTCAAT+TGG | - | chr5_2:48508938-48508957 | Msa0773530:CDS | 30.0% | |
| CTCATTGTTACACTTCAATT+GGG | - | chr5_2:48508939-48508958 | Msa0773530:CDS | 30.0% | |
| TGATTTCTCTCTCTGTAATT+TGG | - | chr5_2:48509002-48509021 | Msa0773530:intron | 30.0% | |
| ATATGATAGGATGAAGATGA+AGG | - | chr5_2:48509426-48509445 | Msa0773530:intron | 30.0% | |
| AAGTACAATTTATGGAGTTG+TGG | - | chr5_2:48509902-48509921 | Msa0773530:intron | 30.0% | |
| !! | CATCATTTCAAGTTGGTAAT+TGG | + | chr5_2:48510302-48510321 | None:intergenic | 30.0% |
| CACAAAGCCATTCAAATTCT+GGG | + | chr5_2:48508677-48508696 | None:intergenic | 35.0% | |
| TCACAAAGCCATTCAAATTC+TGG | + | chr5_2:48508678-48508697 | None:intergenic | 35.0% | |
| !! | TTGAATGGCTTTGTGATAGT+AGG | - | chr5_2:48508682-48508701 | Msa0773530:intron | 35.0% |
| ! | TTTGAGTTTGTTTGTGTTGG+TGG | - | chr5_2:48508792-48508811 | Msa0773530:intron | 35.0% |
| ! | TTGAGTTTGTTTGTGTTGGT+GGG | - | chr5_2:48508793-48508812 | Msa0773530:intron | 35.0% |
| TTATGATGAACAAACTGCGT+TGG | - | chr5_2:48508894-48508913 | Msa0773530:intron | 35.0% | |
| ! | TGGAAAACTGGGTTGAATAT+AGG | - | chr5_2:48508975-48508994 | Msa0773530:intron | 35.0% |
| CTCTCTCTGTAATTTGGAAA+AGG | - | chr5_2:48509008-48509027 | Msa0773530:intron | 35.0% | |
| GAAATTCTTGAGATTGGATG+TGG | - | chr5_2:48509597-48509616 | Msa0773530:intron | 35.0% | |
| GAAAAAAGAGTTAGAGATGC+TGG | - | chr5_2:48509708-48509727 | Msa0773530:intron | 35.0% | |
| ! | GGTTGTTGATTAATACCTGA+AGG | + | chr5_2:48509734-48509753 | None:intergenic | 35.0% |
| TTAAGCATCTAGGTCATGAT+TGG | + | chr5_2:48509842-48509861 | None:intergenic | 35.0% | |
| TTGTCTTCATGTGCAGAATA+AGG | - | chr5_2:48509965-48509984 | Msa0773530:intron | 35.0% | |
| TCAAGTTCTTGCTTATCATC+TGG | + | chr5_2:48510027-48510046 | None:intergenic | 35.0% | |
| TGATAAGCAAGAACTTGATG+AGG | - | chr5_2:48510029-48510048 | Msa0773530:intron | 35.0% | |
| ! | TGATGAGGTTTTACAAAGAG+AGG | - | chr5_2:48510044-48510063 | Msa0773530:intron | 35.0% |
| CACAAAGCCATTCAAATTCT+GGG | + | chr5_2:48508677-48508696 | None:intergenic | 35.0% | |
| TCACAAAGCCATTCAAATTC+TGG | + | chr5_2:48508678-48508697 | None:intergenic | 35.0% | |
| !! | TTGAATGGCTTTGTGATAGT+AGG | - | chr5_2:48508682-48508701 | Msa0773530:intron | 35.0% |
| ! | TTTGAGTTTGTTTGTGTTGG+TGG | - | chr5_2:48508792-48508811 | Msa0773530:intron | 35.0% |
| ! | TTGAGTTTGTTTGTGTTGGT+GGG | - | chr5_2:48508793-48508812 | Msa0773530:intron | 35.0% |
| TTATGATGAACAAACTGCGT+TGG | - | chr5_2:48508894-48508913 | Msa0773530:intron | 35.0% | |
| ! | TGGAAAACTGGGTTGAATAT+AGG | - | chr5_2:48508975-48508994 | Msa0773530:intron | 35.0% |
| CTCTCTCTGTAATTTGGAAA+AGG | - | chr5_2:48509008-48509027 | Msa0773530:intron | 35.0% | |
| GAAATTCTTGAGATTGGATG+TGG | - | chr5_2:48509597-48509616 | Msa0773530:intron | 35.0% | |
| GAAAAAAGAGTTAGAGATGC+TGG | - | chr5_2:48509708-48509727 | Msa0773530:intron | 35.0% | |
| ! | GGTTGTTGATTAATACCTGA+AGG | + | chr5_2:48509734-48509753 | None:intergenic | 35.0% |
| TTAAGCATCTAGGTCATGAT+TGG | + | chr5_2:48509842-48509861 | None:intergenic | 35.0% | |
| TTGTCTTCATGTGCAGAATA+AGG | - | chr5_2:48509965-48509984 | Msa0773530:intron | 35.0% | |
| TCAAGTTCTTGCTTATCATC+TGG | + | chr5_2:48510027-48510046 | None:intergenic | 35.0% | |
| TGATAAGCAAGAACTTGATG+AGG | - | chr5_2:48510029-48510048 | Msa0773530:intron | 35.0% | |
| ! | TGATGAGGTTTTACAAAGAG+AGG | - | chr5_2:48510044-48510063 | Msa0773530:intron | 35.0% |
| TTCAATTGGGCTTATGTGAC+TGG | - | chr5_2:48508952-48508971 | Msa0773530:CDS | 40.0% | |
| TTATGTGACTGGTGGAAAAC+TGG | - | chr5_2:48508963-48508982 | Msa0773530:intron | 40.0% | |
| TATGTGACTGGTGGAAAACT+GGG | - | chr5_2:48508964-48508983 | Msa0773530:intron | 40.0% | |
| GTTGAAGTAGCAAACTCAGT+TGG | - | chr5_2:48509240-48509259 | Msa0773530:intron | 40.0% | |
| ATACTCATGTGCAGTGTTCA+AGG | - | chr5_2:48509298-48509317 | Msa0773530:intron | 40.0% | |
| TTCTTGAGATTGGATGTGGA+TGG | - | chr5_2:48509601-48509620 | Msa0773530:intron | 40.0% | |
| TCTTGAGATTGGATGTGGAT+GGG | - | chr5_2:48509602-48509621 | Msa0773530:intron | 40.0% | |
| AGAACTGGTTGCAAATACAC+CGG | - | chr5_2:48509651-48509670 | Msa0773530:intron | 40.0% | |
| TTGTGGCAATTTCTGCACTA+AGG | - | chr5_2:48509919-48509938 | Msa0773530:intron | 40.0% | |
| GAGTCATCTGAATGGCAAAT+AGG | + | chr5_2:48510110-48510129 | None:intergenic | 40.0% | |
| AACTCAGAGAGTCATCTGAA+TGG | + | chr5_2:48510118-48510137 | None:intergenic | 40.0% | |
| ! | AGCAACGCATCATTTCAAGT+TGG | + | chr5_2:48510309-48510328 | None:intergenic | 40.0% |
| TTCAATTGGGCTTATGTGAC+TGG | - | chr5_2:48508952-48508971 | Msa0773530:CDS | 40.0% | |
| TTATGTGACTGGTGGAAAAC+TGG | - | chr5_2:48508963-48508982 | Msa0773530:intron | 40.0% | |
| TATGTGACTGGTGGAAAACT+GGG | - | chr5_2:48508964-48508983 | Msa0773530:intron | 40.0% | |
| GTTGAAGTAGCAAACTCAGT+TGG | - | chr5_2:48509240-48509259 | Msa0773530:intron | 40.0% | |
| ATACTCATGTGCAGTGTTCA+AGG | - | chr5_2:48509298-48509317 | Msa0773530:intron | 40.0% | |
| TTCTTGAGATTGGATGTGGA+TGG | - | chr5_2:48509601-48509620 | Msa0773530:intron | 40.0% | |
| TCTTGAGATTGGATGTGGAT+GGG | - | chr5_2:48509602-48509621 | Msa0773530:intron | 40.0% | |
| AGAACTGGTTGCAAATACAC+CGG | - | chr5_2:48509651-48509670 | Msa0773530:intron | 40.0% | |
| TTGTGGCAATTTCTGCACTA+AGG | - | chr5_2:48509919-48509938 | Msa0773530:intron | 40.0% | |
| GAGTCATCTGAATGGCAAAT+AGG | + | chr5_2:48510110-48510129 | None:intergenic | 40.0% | |
| AACTCAGAGAGTCATCTGAA+TGG | + | chr5_2:48510118-48510137 | None:intergenic | 40.0% | |
| ! | AGCAACGCATCATTTCAAGT+TGG | + | chr5_2:48510309-48510328 | None:intergenic | 40.0% |
| CTGGGAACCCAGAATTTGAA+TGG | - | chr5_2:48508667-48508686 | Msa0773530:CDS | 45.0% | |
| GAACAAACTGCGTTGGATAG+AGG | - | chr5_2:48508901-48508920 | Msa0773530:CDS | 45.0% | |
| CTGCGTTGGATAGAGGAAAT+TGG | - | chr5_2:48508908-48508927 | Msa0773530:CDS | 45.0% | |
| AATTGGGCTTATGTGACTGG+TGG | - | chr5_2:48508955-48508974 | Msa0773530:CDS | 45.0% | |
| !! | TGCATGCTTGCTGTCTCATT+TGG | - | chr5_2:48509203-48509222 | Msa0773530:intron | 45.0% |
| CTTGAGATTGGATGTGGATG+GGG | - | chr5_2:48509603-48509622 | Msa0773530:intron | 45.0% | |
| GACGAGGAAATTGAAGCTGA+TGG | - | chr5_2:48510274-48510293 | Msa0773530:CDS | 45.0% | |
| CTGGGAACCCAGAATTTGAA+TGG | - | chr5_2:48508667-48508686 | Msa0773530:CDS | 45.0% | |
| GAACAAACTGCGTTGGATAG+AGG | - | chr5_2:48508901-48508920 | Msa0773530:CDS | 45.0% | |
| CTGCGTTGGATAGAGGAAAT+TGG | - | chr5_2:48508908-48508927 | Msa0773530:CDS | 45.0% | |
| AATTGGGCTTATGTGACTGG+TGG | - | chr5_2:48508955-48508974 | Msa0773530:CDS | 45.0% | |
| !! | TGCATGCTTGCTGTCTCATT+TGG | - | chr5_2:48509203-48509222 | Msa0773530:intron | 45.0% |
| CTTGAGATTGGATGTGGATG+GGG | - | chr5_2:48509603-48509622 | Msa0773530:intron | 45.0% | |
| GACGAGGAAATTGAAGCTGA+TGG | - | chr5_2:48510274-48510293 | Msa0773530:CDS | 45.0% | |
| CTCCTCTGACAGAGTGATAC+CGG | + | chr5_2:48509673-48509692 | None:intergenic | 50.0% | |
| !! | AGTTAGAGATGCTGGCCTTC+AGG | - | chr5_2:48509716-48509735 | Msa0773530:intron | 50.0% |
| CTCCTCTGACAGAGTGATAC+CGG | + | chr5_2:48509673-48509692 | None:intergenic | 50.0% | |
| !! | AGTTAGAGATGCTGGCCTTC+AGG | - | chr5_2:48509716-48509735 | Msa0773530:intron | 50.0% |
| CACCGGTATCACTCTGTCAG+AGG | - | chr5_2:48509668-48509687 | Msa0773530:intron | 55.0% | |
| GCAGATGAGGGAAGAAGACG+AGG | - | chr5_2:48510258-48510277 | Msa0773530:CDS | 55.0% | |
| CACCGGTATCACTCTGTCAG+AGG | - | chr5_2:48509668-48509687 | Msa0773530:intron | 55.0% | |
| GCAGATGAGGGAAGAAGACG+AGG | - | chr5_2:48510258-48510277 | Msa0773530:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 48508627 | 48510333 | 48508627 | ID=Msa0773530;Name=Msa0773530 |
| chr5_2 | mRNA | 48508627 | 48510333 | 48508627 | ID=Msa0773530-mRNA-1;Parent=Msa0773530;Name=Msa0773530-mRNA-1;_AED=0.84;_eAED=0.85;_QI=0|0|0|0.33|1|1|3|0|77 |
| chr5_2 | exon | 48510257 | 48510333 | 48510257 | ID=Msa0773530-mRNA-1:exon:14540;Parent=Msa0773530-mRNA-1 |
| chr5_2 | exon | 48508895 | 48508979 | 48508895 | ID=Msa0773530-mRNA-1:exon:14539;Parent=Msa0773530-mRNA-1 |
| chr5_2 | exon | 48508627 | 48508698 | 48508627 | ID=Msa0773530-mRNA-1:exon:14538;Parent=Msa0773530-mRNA-1 |
| chr5_2 | CDS | 48510257 | 48510333 | 48510257 | ID=Msa0773530-mRNA-1:cds;Parent=Msa0773530-mRNA-1 |
| chr5_2 | CDS | 48508895 | 48508979 | 48508895 | ID=Msa0773530-mRNA-1:cds;Parent=Msa0773530-mRNA-1 |
| chr5_2 | CDS | 48508627 | 48508698 | 48508627 | ID=Msa0773530-mRNA-1:cds;Parent=Msa0773530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
