AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0777620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777620 MtrunA17_Chr5g0432901 91.103 281 1 1 1 257 1 281 0.0 516
Msa0777620 MtrunA17_Chr3g0108401 73.764 263 39 3 24 257 1 262 2.84e-137 387
Msa0777620 MtrunA17_Chr7g0245301 64.460 287 60 5 1 254 1 278 3.35e-120 344
Msa0777620 MtrunA17_Chr8g0334451 65.343 277 65 5 3 255 5 274 1.62e-119 342
Msa0777620 MtrunA17_Chr3g0080071 52.364 275 99 5 10 255 20 291 5.20e-87 260
Msa0777620 MtrunA17_Chr2g0276961 50.189 265 94 7 28 255 47 310 4.53e-69 215
Msa0777620 MtrunA17_Chr4g0018581 44.800 125 59 4 111 233 217 333 1.04e-24 102
Msa0777620 MtrunA17_Chr7g0267531 62.121 66 15 2 89 148 20 81 1.55e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777620 AT3G08620.1 60.764 288 73 5 1 255 1 281 1.31e-115 333
Msa0777620 AT2G38610.2 61.644 292 67 8 1 255 1 284 4.50e-111 322
Msa0777620 AT2G38610.1 61.644 292 67 8 1 255 1 284 4.50e-111 322
Msa0777620 AT3G08620.2 60.833 240 57 3 49 255 1 236 3.13e-97 285
Msa0777620 AT5G56140.1 51.590 283 103 8 5 255 32 312 5.57e-86 259
Msa0777620 AT4G26480.3 50.877 285 100 9 5 255 28 306 1.71e-84 254
Msa0777620 AT4G26480.1 50.877 285 100 9 5 255 28 306 1.71e-84 254
Msa0777620 AT1G09660.1 49.813 267 78 4 33 256 44 297 3.05e-79 241
Msa0777620 AT1G09660.3 49.813 267 78 4 33 256 44 297 3.05e-79 241
Msa0777620 AT4G26480.2 64.417 163 57 1 93 255 60 221 9.71e-74 224
Msa0777620 AT1G09660.2 47.685 216 67 2 33 211 44 250 1.15e-60 192
Msa0777620 AT5G51300.1 44.800 125 59 3 111 233 244 360 4.91e-24 101
Msa0777620 AT5G51300.2 44.800 125 59 3 111 233 244 360 4.91e-24 101
Msa0777620 AT5G51300.3 44.800 125 59 3 111 233 244 360 4.91e-24 101

Find 74 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCAATGTTGTTGATTTA+AGG 0.084192 5_2:-56712687 Msa0777620:CDS
AACTTTGTTGGGAGGCTTCT+TGG 0.164659 5_2:-56713115 Msa0777620:CDS
TTGCTTCTCATGTTTATATT+TGG 0.166777 5_2:+56714656 None:intergenic
ATAGAGAGAGACACACTTTA+AGG 0.180017 5_2:-56714996 Msa0777620:five_prime_UTR
GTGATTCTATGTTTCTTAAC+AGG 0.262166 5_2:-56714852 Msa0777620:five_prime_UTR
GAAAGACTATTGGTCAGTTT+TGG 0.283012 5_2:+56711933 None:intergenic
GAATTAGCCCTTGCTGTTCC+TGG 0.348426 5_2:+56714680 None:intergenic
TACTAATCTACTTACATATA+AGG 0.350855 5_2:-56714961 Msa0777620:five_prime_UTR
TTGATGATATGATGTCAATT+TGG 0.353916 5_2:-56711832 Msa0777620:three_prime_UTR
TTGGGAGGCTTCTTGGCCCA+AGG 0.368655 5_2:-56713108 Msa0777620:CDS
ATTTAAGAGTCTAGTACATA+AGG 0.382326 5_2:+56714268 None:intergenic
CGCTTCAGCGAATTACCCCT+TGG 0.397848 5_2:+56713091 None:intergenic
CGCGTATTTATCAGAGGAAA+AGG 0.402670 5_2:-56713046 Msa0777620:CDS
ATCATTTCATTAGAGGTGAA+TGG 0.424912 5_2:+56711964 None:intergenic
ACACAACATTGCTTTGAAGT+TGG 0.426596 5_2:-56711468 Msa0777620:three_prime_UTR
TTAATGTAGAGAAAGACTAT+TGG 0.433629 5_2:+56711923 None:intergenic
TGGGAGGCTTCTTGGCCCAA+GGG 0.460083 5_2:-56713107 Msa0777620:CDS
TCATTCAAGTGCTCAAATCC+TGG 0.465607 5_2:+56712742 None:intergenic
GACTATGACAGAGTGCAGTT+TGG 0.471960 5_2:-56713803 Msa0777620:CDS
TGTACTAGACTCTTAAATCA+AGG 0.476327 5_2:-56714263 Msa0777620:intron
CTACCAACTCAGTTAAGTAT+TGG 0.476363 5_2:+56714330 None:intergenic
TTTAAGAGTCTAGTACATAA+GGG 0.481131 5_2:+56714269 None:intergenic
TTCAATAAAAGACTTCGATA+AGG 0.496581 5_2:-56713023 Msa0777620:intron
ACAAATATCTTACAGTTGGA+TGG 0.499395 5_2:+56713344 None:intergenic
AAAAGAGATGTCTGAGAACA+AGG 0.499405 5_2:+56714813 None:intergenic
TTCAGTTCAACTTTGTTGGG+AGG 0.499629 5_2:-56713123 Msa0777620:intron
CTTAAATCAACAACATTGAC+AGG 0.499752 5_2:+56712688 None:intergenic
GTCAATTTGGAGAAAAGAGA+AGG 0.503388 5_2:-56711819 Msa0777620:three_prime_UTR
AAGCGAGTGGAGGCTACTAC+TGG 0.504350 5_2:-56713073 Msa0777620:CDS
AGAAAGAAACTACTCATTGA+GGG 0.511886 5_2:-56715125 None:intergenic
GAGTTGCTGAGGGGAAGGCC+AGG 0.515410 5_2:-56712760 Msa0777620:CDS
TAGAAAGAAACTACTCATTG+AGG 0.515420 5_2:-56715126 None:intergenic
AGAGTATGTAGAAAATTCAA+AGG 0.522319 5_2:+56714776 None:intergenic
ATTGATAAAAGAGAAGGTAG+TGG 0.525553 5_2:+56714894 None:intergenic
AAGGTAGTGGATGAAAAGAA+AGG 0.533639 5_2:+56714907 None:intergenic
TCAATTTGGAGAAAAGAGAA+GGG 0.535602 5_2:-56711818 Msa0777620:three_prime_UTR
ATCAGGGGCTAAATGTGGAT+TGG 0.537326 5_2:-56713438 Msa0777620:intron
AGGAAGAGTTGCTGAGGGGA+AGG 0.539626 5_2:-56712765 Msa0777620:CDS
GTAGCAGAATATCAGAAGCT+TGG 0.544343 5_2:-56714311 Msa0777620:CDS
AAGGGTAGGACTTGCATGAA+AGG 0.548903 5_2:+56714287 None:intergenic
TTAAGGGCTTCTGGAAAGAG+TGG 0.550881 5_2:-56713851 Msa0777620:CDS
TGTGAAGAAGATGTTGCGCT+TGG 0.554183 5_2:-56713383 Msa0777620:CDS
TGTCATAGTCACTAAACCCT+TGG 0.557531 5_2:+56713816 None:intergenic
GAGTATGTAGAAAATTCAAA+GGG 0.563198 5_2:+56714777 None:intergenic
TAGCCAATACTTAACTGAGT+TGG 0.564535 5_2:-56714333 Msa0777620:CDS
GCTTCAGCGAATTACCCCTT+GGG 0.565469 5_2:+56713092 None:intergenic
ATCTAAAGGAAGAGTTGCTG+AGG 0.572490 5_2:-56712771 Msa0777620:intron
AGCCCTTGCTGTTCCTGGTG+AGG 0.578872 5_2:+56714685 None:intergenic
GGGAGGCTTCTTGGCCCAAG+GGG 0.580940 5_2:-56713106 Msa0777620:CDS
ATGTGAGAATTTGAGACTGC+TGG 0.585989 5_2:+56713406 None:intergenic
AAAATAGAAATATAGATAGA+GGG 0.586267 5_2:+56714935 None:intergenic
GTGGATTGATGCAGAACCAA+GGG 0.591733 5_2:-56713832 Msa0777620:CDS
GGGTAATTCGCTGAAGCGAG+TGG 0.598682 5_2:-56713086 Msa0777620:CDS
ATTACATCAGGGGCTAAATG+TGG 0.599851 5_2:-56713443 Msa0777620:intron
TGTTTGATTGATAAAAGAGA+AGG 0.603229 5_2:+56714888 None:intergenic
GATGTCTGAGAACAAGGGTG+TGG 0.604434 5_2:+56714819 None:intergenic
TCTAAAGGAAGAGTTGCTGA+GGG 0.609826 5_2:-56712770 Msa0777620:intron
GAGAGACTACCACTCAGTTG+AGG 0.613051 5_2:+56711988 None:intergenic
AGAGTCTAGTACATAAGGGT+AGG 0.616507 5_2:+56714273 None:intergenic
ACAGTTGGATGGTTATCATG+AGG 0.620762 5_2:+56713355 None:intergenic
TTTCCTCACCAGGAACAGCA+AGG 0.622680 5_2:-56714688 Msa0777620:CDS
TTCCTCACCAGGAACAGCAA+GGG 0.622720 5_2:-56714687 Msa0777620:CDS
AGTGGATTGATGCAGAACCA+AGG 0.632569 5_2:-56713833 Msa0777620:CDS
GCCTCAATCAAAATGTGCAG+TGG 0.633269 5_2:+56712718 None:intergenic
AACAATCAGATTTCCTCACC+AGG 0.634098 5_2:-56714698 Msa0777620:CDS
GGTTGCCGCGTATTTATCAG+AGG 0.641272 5_2:-56713052 Msa0777620:CDS
CAATTTGGAGAAAAGAGAAG+GGG 0.646981 5_2:-56711817 Msa0777620:three_prime_UTR
AGTCACAAGACATTTACAAG+AGG 0.649466 5_2:-56712062 Msa0777620:CDS
TAATCTACTTACATATAAGG+TGG 0.649861 5_2:-56714958 Msa0777620:five_prime_UTR
AAAGAGATGTCTGAGAACAA+GGG 0.652516 5_2:+56714814 None:intergenic
GAAGAGAGTCCTCAACTGAG+TGG 0.680778 5_2:-56711997 Msa0777620:CDS
TAATTCGCTGAAGCGAGTGG+AGG 0.685966 5_2:-56713083 Msa0777620:CDS
CGAAGAGCTACTCAAACCTG+TGG 0.704906 5_2:-56712641 Msa0777620:intron
CTAAAGGAAGAGTTGCTGAG+GGG 0.733546 5_2:-56712769 Msa0777620:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACACAAAAAAAAAAAAAAA+AGG + chr5_2:56711801-56711820 None:intergenic 10.0%
!! AATAAAAAACCTTTAATATA+TGG + chr5_2:56712318-56712337 None:intergenic 10.0%
!!! ATATTAAAGGTTTTTTATTT+TGG - chr5_2:56712319-56712338 Msa0777620:intron 10.0%
!!! TATTTTACAATTTTTTACAA+TGG + chr5_2:56712947-56712966 None:intergenic 10.0%
!! AAAATAGAAATATAGATAGA+GGG + chr5_2:56711608-56711627 None:intergenic 15.0%
!! AAAAATAGAAATATAGATAG+AGG + chr5_2:56711609-56711628 None:intergenic 15.0%
!!! CATATACATATAACTATTTT+TGG + chr5_2:56712135-56712154 None:intergenic 15.0%
!! ATTCTAAATCCATATATTAA+AGG - chr5_2:56712306-56712325 Msa0777620:intron 15.0%
!!! TTTATTATCAGAGATTTTAA+GGG - chr5_2:56712673-56712692 Msa0777620:CDS 15.0%
!! TAGCAAATTTAATGATTAAA+GGG + chr5_2:56713061-56713080 None:intergenic 15.0%
!! TTAGCAAATTTAATGATTAA+AGG + chr5_2:56713062-56713081 None:intergenic 15.0%
!! TACTAATCTACTTACATATA+AGG - chr5_2:56711579-56711598 Msa0777620:intron 20.0%
!!! TGATGAGTTTAATTAACTTT+TGG - chr5_2:56712158-56712177 Msa0777620:intron 20.0%
!! CTTCTGTCAAATATATATAT+AGG + chr5_2:56712534-56712553 None:intergenic 20.0%
!!! CATATTTAACTACTTTTAGT+TGG - chr5_2:56712606-56712625 Msa0777620:intron 20.0%
!!! CTTTATTATCAGAGATTTTA+AGG - chr5_2:56712672-56712691 Msa0777620:CDS 20.0%
!! ATTAAGTTCAATATTACATC+AGG - chr5_2:56713085-56713104 Msa0777620:CDS 20.0%
!! TTAAGTTCAATATTACATCA+GGG - chr5_2:56713086-56713105 Msa0777620:CDS 20.0%
!! CCTTTAGATTATAACAAAAT+AGG + chr5_2:56713758-56713777 None:intergenic 20.0%
!!! CCTATTTTGTTATAATCTAA+AGG - chr5_2:56713755-56713774 Msa0777620:intron 20.0%
!! ATGAAAAATATAAATACCAC+AGG + chr5_2:56713918-56713937 None:intergenic 20.0%
!!! ATATTTTGATTTTACCAAAC+CGG - chr5_2:56714155-56714174 Msa0777620:intron 20.0%
!! ATATAGTCAAGATATCAATA+CGG - chr5_2:56714346-56714365 Msa0777620:intron 20.0%
!!! TCTTTTTATCATTTCATTAG+AGG + chr5_2:56714586-56714605 None:intergenic 20.0%
! TAATCTACTTACATATAAGG+TGG - chr5_2:56711582-56711601 Msa0777620:intron 25.0%
! TGTTTGATTGATAAAAGAGA+AGG + chr5_2:56711655-56711674 None:intergenic 25.0%
! GAGTATGTAGAAAATTCAAA+GGG + chr5_2:56711766-56711785 None:intergenic 25.0%
! AGAGTATGTAGAAAATTCAA+AGG + chr5_2:56711767-56711786 None:intergenic 25.0%
! TTGCTTCTCATGTTTATATT+TGG + chr5_2:56711887-56711906 None:intergenic 25.0%
! TTTAAGAGTCTAGTACATAA+GGG + chr5_2:56712274-56712293 None:intergenic 25.0%
! ATTTAAGAGTCTAGTACATA+AGG + chr5_2:56712275-56712294 None:intergenic 25.0%
! GATTCAAGTAACATAAAAAG+TGG + chr5_2:56712385-56712404 None:intergenic 25.0%
!!! CTTTTTATGTTACTTGAATC+AGG - chr5_2:56712385-56712404 Msa0777620:intron 25.0%
!! ACTCAGTTTTCTTAATAACT+TGG - chr5_2:56712419-56712438 Msa0777620:intron 25.0%
! TATCTCATATTTGTGTTATG+AGG - chr5_2:56712447-56712466 Msa0777620:intron 25.0%
! TATAAGAACAGACATAAAGT+AGG + chr5_2:56712639-56712658 None:intergenic 25.0%
! GATTACATTCAAACTATTAC+CGG + chr5_2:56712864-56712883 None:intergenic 25.0%
! AATAGAAGAATACAGATCAA+TGG + chr5_2:56712979-56712998 None:intergenic 25.0%
! TGAATGAAAAAGAAATGGAA+GGG + chr5_2:56713016-56713035 None:intergenic 25.0%
! ATGAATGAAAAAGAAATGGA+AGG + chr5_2:56713017-56713036 None:intergenic 25.0%
! GTGAATGAATGAAAAAGAAA+TGG + chr5_2:56713021-56713040 None:intergenic 25.0%
! TAAGTTCAATATTACATCAG+GGG - chr5_2:56713087-56713106 Msa0777620:CDS 25.0%
! TGTTACAAATATCTTACAGT+TGG + chr5_2:56713203-56713222 None:intergenic 25.0%
!!! TGATGTTATTTTTCTACTCT+CGG + chr5_2:56713312-56713331 None:intergenic 25.0%
!! TTTTTCACGTAAAATTCTCT+CGG - chr5_2:56713347-56713366 Msa0777620:intron 25.0%
!! TATTTTCAGTTCAACTTTGT+TGG - chr5_2:56713413-56713432 Msa0777620:CDS 25.0%
!! ATTTTCAGTTCAACTTTGTT+GGG - chr5_2:56713414-56713433 Msa0777620:CDS 25.0%
! TTCAATAAAAGACTTCGATA+AGG - chr5_2:56713517-56713536 Msa0777620:intron 25.0%
! ATCATAAAACCAAATTCGTA+AGG - chr5_2:56713615-56713634 Msa0777620:intron 25.0%
! AACTAACACAAATCAAAAGA+AGG + chr5_2:56713690-56713709 None:intergenic 25.0%
! CATGAAAATGCAGATTATAA+TGG + chr5_2:56714007-56714026 None:intergenic 25.0%
!!! TTCAAGTTACATTTTAAAGC+CGG + chr5_2:56714177-56714196 None:intergenic 25.0%
! TGTATCAAAACTAAACTCTA+AGG - chr5_2:56714393-56714412 Msa0777620:intron 25.0%
!! AAGGAGAGTTAGAAATATTT+TGG + chr5_2:56714436-56714455 None:intergenic 25.0%
! TTAATGTAGAGAAAGACTAT+TGG + chr5_2:56714620-56714639 None:intergenic 25.0%
!! TTGATGATATGATGTCAATT+TGG - chr5_2:56714708-56714727 Msa0777620:CDS 25.0%
!!! ATATTTTGACATGTTTGTTG+AGG - chr5_2:56714834-56714853 Msa0777620:five_prime_UTR 25.0%
!!! TATTTTGACATGTTTGTTGA+GGG - chr5_2:56714835-56714854 Msa0777620:five_prime_UTR 25.0%
ATTGATAAAAGAGAAGGTAG+TGG + chr5_2:56711649-56711668 None:intergenic 30.0%
GTGATTCTATGTTTCTTAAC+AGG - chr5_2:56711688-56711707 Msa0777620:intron 30.0%
! GTTTCTTAACAGGATTTTTG+AGG - chr5_2:56711698-56711717 Msa0777620:intron 30.0%
! TTTTGTGTTGAATTGATGTC+TGG - chr5_2:56711812-56711831 Msa0777620:three_prime_UTR 30.0%
!! GAAGCAATTTTGATGTTGAT+AGG - chr5_2:56711900-56711919 Msa0777620:three_prime_UTR 30.0%
GGTTAAGTCGAATTTATTGT+CGG - chr5_2:56712051-56712070 Msa0777620:CDS 30.0%
TGTACTAGACTCTTAAATCA+AGG - chr5_2:56712277-56712296 Msa0777620:intron 30.0%
! GTAGCACCTTTTATTCTAAA+AGG + chr5_2:56712909-56712928 None:intergenic 30.0%
GAATGAAAAAGAAATGGAAG+GGG + chr5_2:56713015-56713034 None:intergenic 30.0%
ACAAATATCTTACAGTTGGA+TGG + chr5_2:56713199-56713218 None:intergenic 30.0%
ATAACATCAAGAACTGTACA+CGG - chr5_2:56713323-56713342 Msa0777620:intron 30.0%
TAATATTATCTTCGCACCAA+GGG + chr5_2:56713553-56713572 None:intergenic 30.0%
TGCGAAGATAATATTACGAA+TGG - chr5_2:56713557-56713576 Msa0777620:intron 30.0%
ATAAAACCAAATTCGTAAGG+TGG - chr5_2:56713618-56713637 Msa0777620:intron 30.0%
TGAAAACAGGTTATAAAGAG+TGG + chr5_2:56713649-56713668 None:intergenic 30.0%
ACGAGATTAGATTTGAAAAC+AGG + chr5_2:56713662-56713681 None:intergenic 30.0%
CTTAAATCAACAACATTGAC+AGG + chr5_2:56713855-56713874 None:intergenic 30.0%
!! CTGTCAATGTTGTTGATTTA+AGG - chr5_2:56713853-56713872 Msa0777620:CDS 30.0%
! ATAATCTGCATTTTCATGCA+AGG - chr5_2:56714009-56714028 Msa0777620:intron 30.0%
TTACTTTCCGAAGTTAAAAG+AGG - chr5_2:56714051-56714070 Msa0777620:intron 30.0%
!!! GTTTAGTTTTGATACATTGC+CGG + chr5_2:56714389-56714408 None:intergenic 30.0%
!!! GAAATATTTTGGCACAAGAA+TGG + chr5_2:56714425-56714444 None:intergenic 30.0%
ATCATTTCATTAGAGGTGAA+TGG + chr5_2:56714579-56714598 None:intergenic 30.0%
TCAATTTGGAGAAAAGAGAA+GGG - chr5_2:56714722-56714741 Msa0777620:exon 30.0%
ATACTTCAGTGACTTAGAAA+TGG - chr5_2:56714860-56714879 Msa0777620:five_prime_UTR 30.0%
ATACACACACTATCAACAAA+CGG + chr5_2:56715024-56715043 None:intergenic 30.0%
ATAGAGAGAGACACACTTTA+AGG - chr5_2:56711544-56711563 Msa0777620:intron 35.0%
!! AAGGTAGTGGATGAAAAGAA+AGG + chr5_2:56711636-56711655 None:intergenic 35.0%
AAAGAGATGTCTGAGAACAA+GGG + chr5_2:56711729-56711748 None:intergenic 35.0%
AAAAGAGATGTCTGAGAACA+AGG + chr5_2:56711730-56711749 None:intergenic 35.0%
TAGCCAATACTTAACTGAGT+TGG - chr5_2:56712207-56712226 Msa0777620:intron 35.0%
CTACCAACTCAGTTAAGTAT+TGG + chr5_2:56712213-56712232 None:intergenic 35.0%
CTAGACTCTTAAATCAAGGT+TGG - chr5_2:56712281-56712300 Msa0777620:intron 35.0%
! TATGTTACTTGAATCAGGCT+TGG - chr5_2:56712390-56712409 Msa0777620:intron 35.0%
! TCTAAAGAAGGCATAAGACT+AGG + chr5_2:56712773-56712792 None:intergenic 35.0%
CTTGATGTAGTGTCTAAAGA+AGG + chr5_2:56712785-56712804 None:intergenic 35.0%
!! ATTGTTTCTGTTCTATGCAC+CGG - chr5_2:56712842-56712861 Msa0777620:intron 35.0%
!!! GACACGCCTTTTAGAATAAA+AGG - chr5_2:56712900-56712919 Msa0777620:intron 35.0%
! TTTTTCTACTCTCGGACATA+TGG + chr5_2:56713304-56713323 None:intergenic 35.0%
ATTCTTCTACTCTCACAGTT+AGG - chr5_2:56713374-56713393 Msa0777620:CDS 35.0%
! CTTTTCCTCTGATAAATACG+CGG + chr5_2:56713496-56713515 None:intergenic 35.0%
!! ATAAGGTATTTTCTCTCCCT+TGG - chr5_2:56713534-56713553 Msa0777620:intron 35.0%
GTAATATTATCTTCGCACCA+AGG + chr5_2:56713554-56713573 None:intergenic 35.0%
TGGTCTAAATTGATAGACTC+TGG - chr5_2:56713577-56713596 Msa0777620:intron 35.0%
CAAGAAATATTGTTTGCGCA+AGG + chr5_2:56713719-56713738 None:intergenic 35.0%
!!! TCCAAGTCCTCTTTTAACTT+CGG + chr5_2:56714061-56714080 None:intergenic 35.0%
TTCGGCTATTGAATGAAGAT+CGG - chr5_2:56714125-56714144 Msa0777620:intron 35.0%
!!! GTTACATTTTAAAGCCGGTT+TGG + chr5_2:56714172-56714191 None:intergenic 35.0%
!! TCTAAAACAACGAGTTTTCC+AGG + chr5_2:56714289-56714308 None:intergenic 35.0%
GGTTGTAAAATGCTTTACAC+CGG - chr5_2:56714367-56714386 Msa0777620:intron 35.0%
CCTGCACAAAAGAATAACAA+AGG + chr5_2:56714455-56714474 None:intergenic 35.0%
!!! CCTTTGTTATTCTTTTGTGC+AGG - chr5_2:56714452-56714471 Msa0777620:intron 35.0%
AGTCACAAGACATTTACAAG+AGG - chr5_2:56714478-56714497 Msa0777620:intron 35.0%
! GAAAGACTATTGGTCAGTTT+TGG + chr5_2:56714610-56714629 None:intergenic 35.0%
GTCAATTTGGAGAAAAGAGA+AGG - chr5_2:56714721-56714740 Msa0777620:exon 35.0%
CAATTTGGAGAAAAGAGAAG+GGG - chr5_2:56714723-56714742 Msa0777620:exon 35.0%
TACACACACTATCAACAAAC+GGG + chr5_2:56715023-56715042 None:intergenic 35.0%
!! ACACAACATTGCTTTGAAGT+TGG - chr5_2:56715072-56715091 Msa0777620:five_prime_UTR 35.0%
AACAATCAGATTTCCTCACC+AGG - chr5_2:56711842-56711861 Msa0777620:three_prime_UTR 40.0%
ACCAAGCGATTGAACTCAAA+TGG - chr5_2:56712030-56712049 Msa0777620:CDS 40.0%
! ACCATTTGAGTTCAATCGCT+TGG + chr5_2:56712034-56712053 None:intergenic 40.0%
GTATTCCGTAATTCGTCAGA+AGG + chr5_2:56712078-56712097 None:intergenic 40.0%
! AGAGTCATCTTCTAGAGAAC+TGG - chr5_2:56712099-56712118 Msa0777620:intron 40.0%
GTAGCAGAATATCAGAAGCT+TGG - chr5_2:56712229-56712248 Msa0777620:intron 40.0%
AGAGTCTAGTACATAAGGGT+AGG + chr5_2:56712270-56712289 None:intergenic 40.0%
!! TCAGAGATTTTAAGGGCTTC+TGG - chr5_2:56712680-56712699 Msa0777620:CDS 40.0%
TGTCATAGTCACTAAACCCT+TGG + chr5_2:56712727-56712746 None:intergenic 40.0%
GACACTACATCAAGTTTCAC+CGG - chr5_2:56712791-56712810 Msa0777620:intron 40.0%
ATTACATCAGGGGCTAAATG+TGG - chr5_2:56713097-56713116 Msa0777620:CDS 40.0%
! ATGTGAGAATTTGAGACTGC+TGG + chr5_2:56713137-56713156 None:intergenic 40.0%
ACAGTTGGATGGTTATCATG+AGG + chr5_2:56713188-56713207 None:intergenic 40.0%
TTCAGTTCAACTTTGTTGGG+AGG - chr5_2:56713417-56713436 Msa0777620:CDS 40.0%
CGCGTATTTATCAGAGGAAA+AGG - chr5_2:56713494-56713513 Msa0777620:intron 40.0%
ATCTAAAGGAAGAGTTGCTG+AGG - chr5_2:56713769-56713788 Msa0777620:intron 40.0%
TCTAAAGGAAGAGTTGCTGA+GGG - chr5_2:56713770-56713789 Msa0777620:intron 40.0%
TCATTCAAGTGCTCAAATCC+TGG + chr5_2:56713801-56713820 None:intergenic 40.0%
!!! ACCACTGCACATTTTGATTG+AGG - chr5_2:56713821-56713840 Msa0777620:CDS 40.0%
TCCGAAGTTAAAAGAGGACT+TGG - chr5_2:56714057-56714076 Msa0777620:intron 40.0%
ATCACACAGTCAAGTGAGTT+CGG - chr5_2:56714107-56714126 Msa0777620:intron 40.0%
GAGAATCCAATTCCTCTAAG+TGG - chr5_2:56714260-56714279 Msa0777620:intron 40.0%
!! TGACTTAGAAATGGTGCCAT+TGG - chr5_2:56714869-56714888 Msa0777620:five_prime_UTR 40.0%
ATGGAATTGGATGATGCCAT+TGG - chr5_2:56714972-56714991 Msa0777620:five_prime_UTR 40.0%
!! TTCCACTTTGGTGCCATTAA+TGG + chr5_2:56715000-56715019 None:intergenic 40.0%
TCGGACCTTCTGACGAATTA+CGG - chr5_2:56712070-56712089 Msa0777620:intron 45.0%
AAGGGTAGGACTTGCATGAA+AGG + chr5_2:56712256-56712275 None:intergenic 45.0%
! TTAAGGGCTTCTGGAAAGAG+TGG - chr5_2:56712689-56712708 Msa0777620:CDS 45.0%
!! AGTGGATTGATGCAGAACCA+AGG - chr5_2:56712707-56712726 Msa0777620:CDS 45.0%
! GTGGATTGATGCAGAACCAA+GGG - chr5_2:56712708-56712727 Msa0777620:CDS 45.0%
GACTATGACAGAGTGCAGTT+TGG - chr5_2:56712737-56712756 Msa0777620:CDS 45.0%
CTACATCAAGTTTCACCGGA+TGG - chr5_2:56712795-56712814 Msa0777620:intron 45.0%
ATCAGGGGCTAAATGTGGAT+TGG - chr5_2:56713102-56713121 Msa0777620:CDS 45.0%
TGTGAAGAAGATGTTGCGCT+TGG - chr5_2:56713157-56713176 Msa0777620:intron 45.0%
AACTTTGTTGGGAGGCTTCT+TGG - chr5_2:56713425-56713444 Msa0777620:CDS 45.0%
CTAAAGGAAGAGTTGCTGAG+GGG - chr5_2:56713771-56713790 Msa0777620:intron 45.0%
GCCTCAATCAAAATGTGCAG+TGG + chr5_2:56713825-56713844 None:intergenic 45.0%
AAGAGGACTTGGATTTCCTG+CGG - chr5_2:56714068-56714087 Msa0777620:intron 45.0%
ACTTGGATTTCCTGCGGTAA+GGG - chr5_2:56714074-56714093 Msa0777620:intron 45.0%
AGGCTTCCACTTAGAGGAAT+TGG + chr5_2:56714269-56714288 None:intergenic 45.0%
AATTCCTCTAAGTGGAAGCC+TGG - chr5_2:56714268-56714287 Msa0777620:CDS 45.0%
! TTTTCCAGGCTTCCACTTAG+AGG + chr5_2:56714275-56714294 None:intergenic 45.0%
!! GATGCCATTGGTGCCATTAA+TGG - chr5_2:56714984-56715003 Msa0777620:five_prime_UTR 45.0%
TGCCATTAATGGCACCAAAG+TGG - chr5_2:56714995-56715014 Msa0777620:five_prime_UTR 45.0%
! TCAACAAACGGGTTCCACTT+TGG + chr5_2:56715012-56715031 None:intergenic 45.0%
GATGTCTGAGAACAAGGGTG+TGG + chr5_2:56711724-56711743 None:intergenic 50.0%
TTTCCTCACCAGGAACAGCA+AGG - chr5_2:56711852-56711871 Msa0777620:three_prime_UTR 50.0%
TTCCTCACCAGGAACAGCAA+GGG - chr5_2:56711853-56711872 Msa0777620:three_prime_UTR 50.0%
!! GAATTAGCCCTTGCTGTTCC+TGG + chr5_2:56711863-56711882 None:intergenic 50.0%
ATGCGACAAGCTGTTCCATC+CGG + chr5_2:56712813-56712832 None:intergenic 50.0%
GCTTCAGCGAATTACCCCTT+GGG + chr5_2:56713451-56713470 None:intergenic 50.0%
TAATTCGCTGAAGCGAGTGG+AGG - chr5_2:56713457-56713476 Msa0777620:intron 50.0%
GGTTGCCGCGTATTTATCAG+AGG - chr5_2:56713488-56713507 Msa0777620:intron 50.0%
GCGCTTCCACCTTACGAATT+TGG + chr5_2:56713627-56713646 None:intergenic 50.0%
CGAAGAGCTACTCAAACCTG+TGG - chr5_2:56713899-56713918 Msa0777620:intron 50.0%
GACTTGGATTTCCTGCGGTA+AGG - chr5_2:56714073-56714092 Msa0777620:intron 50.0%
GAAGAGAGTCCTCAACTGAG+TGG - chr5_2:56714543-56714562 Msa0777620:intron 50.0%
GAGAGACTACCACTCAGTTG+AGG + chr5_2:56714555-56714574 None:intergenic 50.0%
GATCTCTGCTATGGCACCAA+TGG + chr5_2:56714888-56714907 None:intergenic 50.0%
AATGGCCAGGATCTCTGCTA+TGG + chr5_2:56714897-56714916 None:intergenic 50.0%
ATCCTGGCCATTGATGTGTG+TGG - chr5_2:56714905-56714924 Msa0777620:five_prime_UTR 50.0%
TCCTGGCCATTGATGTGTGT+GGG - chr5_2:56714906-56714925 Msa0777620:five_prime_UTR 50.0%
GTGTGTGATGTGTGTGTGTG+TGG - chr5_2:56714944-56714963 Msa0777620:five_prime_UTR 50.0%
TGTGATGTGTGTGTGTGTGG+TGG - chr5_2:56714947-56714966 Msa0777620:five_prime_UTR 50.0%
! GTGTGTGGTGGAGATGGAAT+TGG - chr5_2:56714959-56714978 Msa0777620:five_prime_UTR 50.0%
!! GGTGCCATTAATGGCACCAA+TGG + chr5_2:56714993-56715012 None:intergenic 50.0%
CGCTTCAGCGAATTACCCCT+TGG + chr5_2:56713452-56713471 None:intergenic 55.0%
GGGTAATTCGCTGAAGCGAG+TGG - chr5_2:56713454-56713473 Msa0777620:intron 55.0%
AAGCGAGTGGAGGCTACTAC+TGG - chr5_2:56713467-56713486 Msa0777620:intron 55.0%
AGGAAGAGTTGCTGAGGGGA+AGG - chr5_2:56713775-56713794 Msa0777620:intron 55.0%
TGCACACTTTCCCTTACCGC+AGG + chr5_2:56714087-56714106 None:intergenic 55.0%
!! TGGTGCCATAGCAGAGATCC+TGG - chr5_2:56714889-56714908 Msa0777620:five_prime_UTR 55.0%
ACCCACACACATCAATGGCC+AGG + chr5_2:56714910-56714929 None:intergenic 55.0%
ATCACACACACATCCCCACG+CGG + chr5_2:56714933-56714952 None:intergenic 55.0%
! GTGTGTGTGTGTGGTGGAGA+TGG - chr5_2:56714953-56714972 Msa0777620:five_prime_UTR 55.0%
!! AGCCCTTGCTGTTCCTGGTG+AGG + chr5_2:56711858-56711877 None:intergenic 60.0%
TTGGGAGGCTTCTTGGCCCA+AGG - chr5_2:56713432-56713451 Msa0777620:CDS 60.0%
TGGGAGGCTTCTTGGCCCAA+GGG - chr5_2:56713433-56713452 Msa0777620:CDS 60.0%
CGCGGACCCACACACATCAA+TGG + chr5_2:56714915-56714934 None:intergenic 60.0%
TTGATGTGTGTGGGTCCGCG+TGG - chr5_2:56714915-56714934 Msa0777620:five_prime_UTR 60.0%
TGATGTGTGTGGGTCCGCGT+GGG - chr5_2:56714916-56714935 Msa0777620:five_prime_UTR 60.0%
GGGAGGCTTCTTGGCCCAAG+GGG - chr5_2:56713434-56713453 Msa0777620:CDS 65.0%
GAGTTGCTGAGGGGAAGGCC+AGG - chr5_2:56713780-56713799 Msa0777620:intron 65.0%
GATGTGTGTGGGTCCGCGTG+GGG - chr5_2:56714917-56714936 Msa0777620:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 56711425 56715137 56711425 ID=Msa0777620;Name=Msa0777620
chr5_2 mRNA 56711425 56715137 56711425 ID=Msa0777620-mRNA-1;Parent=Msa0777620;Name=Msa0777620-mRNA-1;_AED=0.19;_eAED=0.19;_QI=402|0.85|0.87|1|0.57|0.62|8|336|257
chr5_2 exon 56711425 56711527 56711425 ID=Msa0777620-mRNA-1:exon:16806;Parent=Msa0777620-mRNA-1
chr5_2 exon 56711706 56712088 56711706 ID=Msa0777620-mRNA-1:exon:16805;Parent=Msa0777620-mRNA-1
chr5_2 exon 56712642 56712785 56712642 ID=Msa0777620-mRNA-1:exon:16804;Parent=Msa0777620-mRNA-1
chr5_2 exon 56713024 56713140 56713024 ID=Msa0777620-mRNA-1:exon:16803;Parent=Msa0777620-mRNA-1
chr5_2 exon 56713357 56713455 56713357 ID=Msa0777620-mRNA-1:exon:16802;Parent=Msa0777620-mRNA-1
chr5_2 exon 56713799 56713878 56713799 ID=Msa0777620-mRNA-1:exon:16801;Parent=Msa0777620-mRNA-1
chr5_2 exon 56714264 56714352 56714264 ID=Msa0777620-mRNA-1:exon:16800;Parent=Msa0777620-mRNA-1
chr5_2 exon 56714641 56715137 56714641 ID=Msa0777620-mRNA-1:exon:16799;Parent=Msa0777620-mRNA-1
chr5_2 five_prime_UTR 56714736 56715137 56714736 ID=Msa0777620-mRNA-1:five_prime_utr;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56714641 56714735 56714641 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56714264 56714352 56714264 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56713799 56713878 56713799 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56713357 56713455 56713357 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56713024 56713140 56713024 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56712642 56712785 56712642 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 CDS 56711939 56712088 56711939 ID=Msa0777620-mRNA-1:cds;Parent=Msa0777620-mRNA-1
chr5_2 three_prime_UTR 56711706 56711938 56711706 ID=Msa0777620-mRNA-1:three_prime_utr;Parent=Msa0777620-mRNA-1
chr5_2 three_prime_UTR 56711425 56711527 56711425 ID=Msa0777620-mRNA-1:three_prime_utr;Parent=Msa0777620-mRNA-1
Gene Sequence

>Msa0777620

ATGTCTGGTTTGTACAACAATCAGATTTCCTCACCAGGAACAGCAAGGGCTAATTCACCAAATATAAACATGAGAAGCAATTTTGATGTTGATAGCCAATACTTAACTGAGTTGGTAGCAGAATATCAGAAGCTTGGACCTTTCATGCAAGTCCTACCCTTATGTACTAGACTCTTAAATCAAGAGATTTTAAGGGCTTCTGGAAAGAGTGGATTGATGCAGAACCAAGGGTTTAGTGACTATGACAGAGTGCAGTTTGGCAGTGGGCTAAATGTGGATTGGCAAAGAGCACCAGCAGTCTCAAATTCTCACATTGTGAAGAAGATGTTGCGCTTGGATATTCCTCATGATAACCATCCAACTTTCAACTTTGTTGGGAGGCTTCTTGGCCCAAGGGGTAATTCGCTGAAGCGAGTGGAGGCTACTACTGGTTGCCGCGTATTTATCAGAGGAAAAGGTTCAATAAAAGACTTCGATAAGGAAGAGTTGCTGAGGGGAAGGCCAGGATTTGAGCACTTGAATGAACCACTGCACATTTTGATTGAGGCTGAGCTACCTGTCAATGTTGTTGATTTAAGGTTGAGACAAGCACAAGAAATCATCGAAGAGCTACTCAAACCTGTGGATGAGTCACAAGACATTTACAAGAGGCAACAACTTAGAGAACTTGCAATGCTGAATTCAAGTTTTAGAGAAGAGAGTCCTCAACTGAGTGGTAGTCTCTCTCCATTCACCTCTAATGAAATGATAAAAAGAGCCAAAACTGACCAATAG
Protein sequence

>Msa0777620

MSGLYNNQISSPGTARANSPNINMRSNFDVDSQYLTELVAEYQKLGPFMQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSGLNVDWQRAPAVSNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRLRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQLSGSLSPFTSNEMIKRAKTDQ*