AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0778710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0778710 MtrunA17_Chr4g0042631 72.000 50 12 1 1 50 61 108 5.79e-19 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAGAAGCTATTTGGAAAT+TGG 0.198762 5_2:+59526803 Msa0778710:three_prime_UTR
AGTTACTTTGCTAAATAAAT+TGG 0.204645 5_2:+59527073 Msa0778710:three_prime_UTR
CCTTATTTGCAATTATAAAA+AGG 0.205647 5_2:+59527139 Msa0778710:three_prime_UTR
TATTATAATCAGAAGCTATT+TGG 0.227740 5_2:+59526796 Msa0778710:three_prime_UTR
TTGGTAAGATGGTATGTAAA+AGG 0.298662 5_2:+59527092 Msa0778710:three_prime_UTR
ACAGCTGCGGACATTTCTTC+TGG 0.305832 5_2:+59526686 Msa0778710:three_prime_UTR
TCTACAGGCATTGGCGAAAA+TGG 0.313793 5_2:+59527011 Msa0778710:three_prime_UTR
GCTTCGTTTGCAAGAGGGTT+TGG 0.334355 5_2:+59526309 Msa0778710:CDS
AGCTATTTGGAAATTGGTTA+TGG 0.340909 5_2:+59526809 Msa0778710:three_prime_UTR
TTGTTCTTGCCATCAACTTA+CGG 0.354553 5_2:-59526896 None:intergenic
TGTTCTTGCCATCAACTTAC+GGG 0.358089 5_2:-59526895 None:intergenic
AGATTGACAGAGTTGTTAGA+AGG 0.366557 5_2:+59525952 Msa0778710:five_prime_UTR
AAATCTGCATGGAGAGGAAT+TGG 0.385757 5_2:+59526525 Msa0778710:three_prime_UTR
CAGCTACCTCAACCTCATTC+TGG 0.392976 5_2:-59526371 None:intergenic
TGCTAAATAAATTGGTAAGA+TGG 0.398439 5_2:+59527081 Msa0778710:three_prime_UTR
AATAAGGCATCCACTTGAAT+AGG 0.407111 5_2:-59527123 None:intergenic
GTGCAACGCCCGTAAGTTGA+TGG 0.418678 5_2:+59526887 Msa0778710:three_prime_UTR
ACAAAATTGCCTCCATCTAC+AGG 0.450698 5_2:+59526996 Msa0778710:three_prime_UTR
CTAACAACTCTGTCAATCTC+TGG 0.451922 5_2:-59525948 None:intergenic
TGGTAAGATGGTATGTAAAA+GGG 0.453244 5_2:+59527093 Msa0778710:three_prime_UTR
ACCTCAACCTCATTCTGGTT+CGG 0.468186 5_2:-59526366 None:intergenic
TACCGAGTTTCTGTACTCAT+TGG 0.471215 5_2:-59526599 None:intergenic
CACGGGAGCAGCTAACAATA+AGG 0.482265 5_2:+59526935 Msa0778710:three_prime_UTR
GATTGACAGAGTTGTTAGAA+GGG 0.495391 5_2:+59525953 Msa0778710:five_prime_UTR
CTTCCTGCTTCGTTTGCAAG+AGG 0.503520 5_2:+59526303 Msa0778710:CDS
TTTGTCTTCAACACCAACAT+TGG 0.503993 5_2:-59526978 None:intergenic
TTACGGGCGTTGCACAGAGC+TGG 0.507146 5_2:-59526879 None:intergenic
TTGCCTCCATCTACAGGCAT+TGG 0.510059 5_2:+59527002 Msa0778710:three_prime_UTR
AACCCTCTTGCAAACGAAGC+AGG 0.515959 5_2:-59526306 None:intergenic
AGAATGTTGTTGCAGAATCA+AGG 0.516563 5_2:+59526574 Msa0778710:three_prime_UTR
GTCTTCAACACCAACATTGG+AGG 0.518617 5_2:-59526975 None:intergenic
AAGATCAAACTTGGTGTCAC+GGG 0.530779 5_2:+59526918 Msa0778710:three_prime_UTR
GGACATTTCTTCTGGAGTGT+TGG 0.533907 5_2:+59526694 Msa0778710:three_prime_UTR
TTCCTGCTTCGTTTGCAAGA+GGG 0.541204 5_2:+59526304 Msa0778710:CDS
TCCGAACCAGAATGAGGTTG+AGG 0.555820 5_2:+59526365 Msa0778710:CDS
TCTTTGTGCACCTCCAATGT+TGG 0.556229 5_2:+59526965 Msa0778710:three_prime_UTR
TTGACAGTTGAAGGTAATGC+TGG 0.556688 5_2:+59525924 None:intergenic
GGGTTAGTTGCCTATTCAAG+TGG 0.558614 5_2:+59527113 Msa0778710:three_prime_UTR
GCAAGAACAAAGATCAAACT+TGG 0.571276 5_2:+59526909 Msa0778710:three_prime_UTR
AAAGATCAAACTTGGTGTCA+CGG 0.572250 5_2:+59526917 Msa0778710:three_prime_UTR
TCGCCAATGCCTGTAGATGG+AGG 0.609903 5_2:-59527005 None:intergenic
GTGGTTAGATATACGTGTGT+TGG 0.614493 5_2:+59526749 Msa0778710:three_prime_UTR
CTCCGTGGAGGATTGAAGCA+AGG 0.615258 5_2:-59526553 None:intergenic
ATCCTTGCTTCAATCCTCCA+CGG 0.617427 5_2:+59526551 Msa0778710:three_prime_UTR
ACGTGATCCGAACCAGAATG+AGG 0.623333 5_2:+59526359 Msa0778710:CDS
TGTTTGTGTAGCGTAAGGGA+AGG 0.626170 5_2:+59526280 Msa0778710:intron
TGCCAATGAGTACAGAAACT+CGG 0.640910 5_2:+59526597 Msa0778710:three_prime_UTR
TTCTGCAACAACATTCTCCG+TGG 0.648161 5_2:-59526568 None:intergenic
TGCAACAACATTCTCCGTGG+AGG 0.648705 5_2:-59526565 None:intergenic
CATGTCATACTCCATGTGGA+AGG 0.657614 5_2:-59525994 None:intergenic
TAACTTGAGAACCTTCCACA+TGG 0.662862 5_2:+59525983 Msa0778710:exon
GGTTAGAAATCTGCATGGAG+AGG 0.664054 5_2:+59526519 Msa0778710:three_prime_UTR
AGCACATGTCATACTCCATG+TGG 0.685350 5_2:-59525998 None:intergenic
TGAGTGTTTGTGTAGCGTAA+GGG 0.688220 5_2:+59526276 Msa0778710:intron
TGTCAAGGAGTTAACAGCTG+CGG 0.688752 5_2:+59526673 Msa0778710:three_prime_UTR
ATGGTGGTTAGAAATCTGCA+TGG 0.704267 5_2:+59526514 Msa0778710:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATCATAATATACATCAAA+TGG - chr5_2:59526192-59526211 None:intergenic 20.0%
!!! AACATAAAAGAAAAAGTGTA+AGG - chr5_2:59526729-59526748 None:intergenic 20.0%
!! TATTATAATCAGAAGCTATT+TGG + chr5_2:59526796-59526815 Msa0778710:three_prime_UTR 20.0%
!!! AGTTACTTTGCTAAATAAAT+TGG + chr5_2:59527073-59527092 Msa0778710:three_prime_UTR 20.0%
!!! CCTTTTTATAATTGCAAATA+AGG - chr5_2:59527142-59527161 None:intergenic 20.0%
!!! CCTTATTTGCAATTATAAAA+AGG + chr5_2:59527139-59527158 Msa0778710:three_prime_UTR 20.0%
!! AGATCATAATATACATCAAA+TGG - chr5_2:59526192-59526211 None:intergenic 20.0%
!!! AACATAAAAGAAAAAGTGTA+AGG - chr5_2:59526729-59526748 None:intergenic 20.0%
!! TATTATAATCAGAAGCTATT+TGG + chr5_2:59526796-59526815 Msa0778710:three_prime_UTR 20.0%
!!! AGTTACTTTGCTAAATAAAT+TGG + chr5_2:59527073-59527092 Msa0778710:three_prime_UTR 20.0%
!!! CCTTTTTATAATTGCAAATA+AGG - chr5_2:59527142-59527161 None:intergenic 20.0%
!!! CCTTATTTGCAATTATAAAA+AGG + chr5_2:59527139-59527158 Msa0778710:three_prime_UTR 20.0%
!! TCCCTTATTTATTCCATATT+TGG + chr5_2:59526133-59526152 Msa0778710:intron 25.0%
! GCCAAATATGGAATAAATAA+GGG - chr5_2:59526137-59526156 None:intergenic 25.0%
! AGCCAAATATGGAATAAATA+AGG - chr5_2:59526138-59526157 None:intergenic 25.0%
! TTATTTATTCCATATTTGGC+TGG + chr5_2:59526137-59526156 Msa0778710:intron 25.0%
!!! ACATTTTTAAAGAGCTTTTG+TGG + chr5_2:59526436-59526455 Msa0778710:three_prime_UTR 25.0%
!!! CATTTTTAAAGAGCTTTTGT+GGG + chr5_2:59526437-59526456 Msa0778710:three_prime_UTR 25.0%
! CAATTGTGATAGAAAAAAGT+GGG - chr5_2:59526655-59526674 None:intergenic 25.0%
! ACAATTGTGATAGAAAAAAG+TGG - chr5_2:59526656-59526675 None:intergenic 25.0%
!! TTTTTCTATCACAATTGTCA+AGG + chr5_2:59526658-59526677 Msa0778710:three_prime_UTR 25.0%
!!! ACACTTTTTCTTTTATGTTG+TGG + chr5_2:59526730-59526749 Msa0778710:three_prime_UTR 25.0%
! TGCTAAATAAATTGGTAAGA+TGG + chr5_2:59527081-59527100 Msa0778710:three_prime_UTR 25.0%
!! TCCCTTATTTATTCCATATT+TGG + chr5_2:59526133-59526152 Msa0778710:intron 25.0%
! GCCAAATATGGAATAAATAA+GGG - chr5_2:59526137-59526156 None:intergenic 25.0%
! AGCCAAATATGGAATAAATA+AGG - chr5_2:59526138-59526157 None:intergenic 25.0%
! TTATTTATTCCATATTTGGC+TGG + chr5_2:59526137-59526156 Msa0778710:intron 25.0%
!!! ACATTTTTAAAGAGCTTTTG+TGG + chr5_2:59526436-59526455 Msa0778710:three_prime_UTR 25.0%
!!! CATTTTTAAAGAGCTTTTGT+GGG + chr5_2:59526437-59526456 Msa0778710:three_prime_UTR 25.0%
! CAATTGTGATAGAAAAAAGT+GGG - chr5_2:59526655-59526674 None:intergenic 25.0%
! ACAATTGTGATAGAAAAAAG+TGG - chr5_2:59526656-59526675 None:intergenic 25.0%
!! TTTTTCTATCACAATTGTCA+AGG + chr5_2:59526658-59526677 Msa0778710:three_prime_UTR 25.0%
!!! ACACTTTTTCTTTTATGTTG+TGG + chr5_2:59526730-59526749 Msa0778710:three_prime_UTR 25.0%
! TGCTAAATAAATTGGTAAGA+TGG + chr5_2:59527081-59527100 Msa0778710:three_prime_UTR 25.0%
TTCCAATAAACTAAACAGCA+TGG - chr5_2:59526074-59526093 None:intergenic 30.0%
TCCATTATAGCAACTAACTA+AGG - chr5_2:59526165-59526184 None:intergenic 30.0%
TAGCTGTTGAAAAAGAAGAA+CGG + chr5_2:59526388-59526407 Msa0778710:CDS 30.0%
!!! AGGTTTATTTTACTGATGGT+TGG + chr5_2:59526413-59526432 Msa0778710:exon 30.0%
! AAATCGACATCTTTTTCTGA+TGG + chr5_2:59526495-59526514 Msa0778710:three_prime_UTR 30.0%
AAAAATGCATCTCAAGTTAG+AGG - chr5_2:59526775-59526794 None:intergenic 30.0%
ATCAGAAGCTATTTGGAAAT+TGG + chr5_2:59526803-59526822 Msa0778710:three_prime_UTR 30.0%
AGCTATTTGGAAATTGGTTA+TGG + chr5_2:59526809-59526828 Msa0778710:three_prime_UTR 30.0%
TTGGTAAGATGGTATGTAAA+AGG + chr5_2:59527092-59527111 Msa0778710:three_prime_UTR 30.0%
TGGTAAGATGGTATGTAAAA+GGG + chr5_2:59527093-59527112 Msa0778710:three_prime_UTR 30.0%
TTCCAATAAACTAAACAGCA+TGG - chr5_2:59526074-59526093 None:intergenic 30.0%
TCCATTATAGCAACTAACTA+AGG - chr5_2:59526165-59526184 None:intergenic 30.0%
TAGCTGTTGAAAAAGAAGAA+CGG + chr5_2:59526388-59526407 Msa0778710:CDS 30.0%
!!! AGGTTTATTTTACTGATGGT+TGG + chr5_2:59526413-59526432 Msa0778710:exon 30.0%
! AAATCGACATCTTTTTCTGA+TGG + chr5_2:59526495-59526514 Msa0778710:three_prime_UTR 30.0%
AAAAATGCATCTCAAGTTAG+AGG - chr5_2:59526775-59526794 None:intergenic 30.0%
ATCAGAAGCTATTTGGAAAT+TGG + chr5_2:59526803-59526822 Msa0778710:three_prime_UTR 30.0%
AGCTATTTGGAAATTGGTTA+TGG + chr5_2:59526809-59526828 Msa0778710:three_prime_UTR 30.0%
TTGGTAAGATGGTATGTAAA+AGG + chr5_2:59527092-59527111 Msa0778710:three_prime_UTR 30.0%
TGGTAAGATGGTATGTAAAA+GGG + chr5_2:59527093-59527112 Msa0778710:three_prime_UTR 30.0%
AGATTGACAGAGTTGTTAGA+AGG + chr5_2:59525952-59525971 Msa0778710:five_prime_UTR 35.0%
GATTGACAGAGTTGTTAGAA+GGG + chr5_2:59525953-59525972 Msa0778710:five_prime_UTR 35.0%
! TGCCATGCTGTTTAGTTTAT+TGG + chr5_2:59526069-59526088 Msa0778710:intron 35.0%
AAGGGATTTATTGACCTTCA+AGG - chr5_2:59526119-59526138 None:intergenic 35.0%
GCCTTAGTTAGTTGCTATAA+TGG + chr5_2:59526161-59526180 Msa0778710:intron 35.0%
TCATCAGTGAGCAAATACTT+TGG - chr5_2:59526259-59526278 None:intergenic 35.0%
AGCTGTTGAAAAAGAAGAAC+GGG + chr5_2:59526389-59526408 Msa0778710:CDS 35.0%
!!! GGGAAGGTTTATTTTACTGA+TGG + chr5_2:59526409-59526428 Msa0778710:CDS 35.0%
! AAAGAGCTTTTGTGGGATTT+TGG + chr5_2:59526444-59526463 Msa0778710:three_prime_UTR 35.0%
! AAGAGCTTTTGTGGGATTTT+GGG + chr5_2:59526445-59526464 Msa0778710:three_prime_UTR 35.0%
AGAATGTTGTTGCAGAATCA+AGG + chr5_2:59526574-59526593 Msa0778710:three_prime_UTR 35.0%
GAAAAAAGTGGGAGGAAAAA+TGG - chr5_2:59526644-59526663 None:intergenic 35.0%
TTGTGATAGAAAAAAGTGGG+AGG - chr5_2:59526652-59526671 None:intergenic 35.0%
! GATTTGTCTTGCGTATTTTC+TGG + chr5_2:59526849-59526868 Msa0778710:three_prime_UTR 35.0%
TTGTTCTTGCCATCAACTTA+CGG - chr5_2:59526899-59526918 None:intergenic 35.0%
GCAAGAACAAAGATCAAACT+TGG + chr5_2:59526909-59526928 Msa0778710:three_prime_UTR 35.0%
!! AAAGATCAAACTTGGTGTCA+CGG + chr5_2:59526917-59526936 Msa0778710:three_prime_UTR 35.0%
TTTGTCTTCAACACCAACAT+TGG - chr5_2:59526981-59527000 None:intergenic 35.0%
! AATAAGGCATCCACTTGAAT+AGG - chr5_2:59527126-59527145 None:intergenic 35.0%
AGATTGACAGAGTTGTTAGA+AGG + chr5_2:59525952-59525971 Msa0778710:five_prime_UTR 35.0%
GATTGACAGAGTTGTTAGAA+GGG + chr5_2:59525953-59525972 Msa0778710:five_prime_UTR 35.0%
! TGCCATGCTGTTTAGTTTAT+TGG + chr5_2:59526069-59526088 Msa0778710:intron 35.0%
AAGGGATTTATTGACCTTCA+AGG - chr5_2:59526119-59526138 None:intergenic 35.0%
GCCTTAGTTAGTTGCTATAA+TGG + chr5_2:59526161-59526180 Msa0778710:intron 35.0%
TCATCAGTGAGCAAATACTT+TGG - chr5_2:59526259-59526278 None:intergenic 35.0%
AGCTGTTGAAAAAGAAGAAC+GGG + chr5_2:59526389-59526408 Msa0778710:CDS 35.0%
!!! GGGAAGGTTTATTTTACTGA+TGG + chr5_2:59526409-59526428 Msa0778710:CDS 35.0%
! AAAGAGCTTTTGTGGGATTT+TGG + chr5_2:59526444-59526463 Msa0778710:three_prime_UTR 35.0%
! AAGAGCTTTTGTGGGATTTT+GGG + chr5_2:59526445-59526464 Msa0778710:three_prime_UTR 35.0%
AGAATGTTGTTGCAGAATCA+AGG + chr5_2:59526574-59526593 Msa0778710:three_prime_UTR 35.0%
GAAAAAAGTGGGAGGAAAAA+TGG - chr5_2:59526644-59526663 None:intergenic 35.0%
TTGTGATAGAAAAAAGTGGG+AGG - chr5_2:59526652-59526671 None:intergenic 35.0%
! GATTTGTCTTGCGTATTTTC+TGG + chr5_2:59526849-59526868 Msa0778710:three_prime_UTR 35.0%
TTGTTCTTGCCATCAACTTA+CGG - chr5_2:59526899-59526918 None:intergenic 35.0%
GCAAGAACAAAGATCAAACT+TGG + chr5_2:59526909-59526928 Msa0778710:three_prime_UTR 35.0%
!! AAAGATCAAACTTGGTGTCA+CGG + chr5_2:59526917-59526936 Msa0778710:three_prime_UTR 35.0%
TTTGTCTTCAACACCAACAT+TGG - chr5_2:59526981-59527000 None:intergenic 35.0%
! AATAAGGCATCCACTTGAAT+AGG - chr5_2:59527126-59527145 None:intergenic 35.0%
CTAACAACTCTGTCAATCTC+TGG - chr5_2:59525951-59525970 None:intergenic 40.0%
TAACTTGAGAACCTTCCACA+TGG + chr5_2:59525983-59526002 Msa0778710:exon 40.0%
ATGAGTGTTTGTGTAGCGTA+AGG + chr5_2:59526275-59526294 Msa0778710:intron 40.0%
TGAGTGTTTGTGTAGCGTAA+GGG + chr5_2:59526276-59526295 Msa0778710:intron 40.0%
GTTGAAAAAGAAGAACGGGA+AGG + chr5_2:59526393-59526412 Msa0778710:CDS 40.0%
! TCGACATCTTTTTCTGATGG+TGG + chr5_2:59526498-59526517 Msa0778710:three_prime_UTR 40.0%
ATGGTGGTTAGAAATCTGCA+TGG + chr5_2:59526514-59526533 Msa0778710:three_prime_UTR 40.0%
AAATCTGCATGGAGAGGAAT+TGG + chr5_2:59526525-59526544 Msa0778710:three_prime_UTR 40.0%
TGCCAATGAGTACAGAAACT+CGG + chr5_2:59526597-59526616 Msa0778710:three_prime_UTR 40.0%
TACCGAGTTTCTGTACTCAT+TGG - chr5_2:59526602-59526621 None:intergenic 40.0%
GTGGTTAGATATACGTGTGT+TGG + chr5_2:59526749-59526768 Msa0778710:three_prime_UTR 40.0%
TGTTCTTGCCATCAACTTAC+GGG - chr5_2:59526898-59526917 None:intergenic 40.0%
!! AAGATCAAACTTGGTGTCAC+GGG + chr5_2:59526918-59526937 Msa0778710:three_prime_UTR 40.0%
ACAAAATTGCCTCCATCTAC+AGG + chr5_2:59526996-59527015 Msa0778710:three_prime_UTR 40.0%
CTAACAACTCTGTCAATCTC+TGG - chr5_2:59525951-59525970 None:intergenic 40.0%
TAACTTGAGAACCTTCCACA+TGG + chr5_2:59525983-59526002 Msa0778710:exon 40.0%
ATGAGTGTTTGTGTAGCGTA+AGG + chr5_2:59526275-59526294 Msa0778710:intron 40.0%
TGAGTGTTTGTGTAGCGTAA+GGG + chr5_2:59526276-59526295 Msa0778710:intron 40.0%
GTTGAAAAAGAAGAACGGGA+AGG + chr5_2:59526393-59526412 Msa0778710:CDS 40.0%
! TCGACATCTTTTTCTGATGG+TGG + chr5_2:59526498-59526517 Msa0778710:three_prime_UTR 40.0%
ATGGTGGTTAGAAATCTGCA+TGG + chr5_2:59526514-59526533 Msa0778710:three_prime_UTR 40.0%
AAATCTGCATGGAGAGGAAT+TGG + chr5_2:59526525-59526544 Msa0778710:three_prime_UTR 40.0%
TGCCAATGAGTACAGAAACT+CGG + chr5_2:59526597-59526616 Msa0778710:three_prime_UTR 40.0%
TACCGAGTTTCTGTACTCAT+TGG - chr5_2:59526602-59526621 None:intergenic 40.0%
GTGGTTAGATATACGTGTGT+TGG + chr5_2:59526749-59526768 Msa0778710:three_prime_UTR 40.0%
TGTTCTTGCCATCAACTTAC+GGG - chr5_2:59526898-59526917 None:intergenic 40.0%
!! AAGATCAAACTTGGTGTCAC+GGG + chr5_2:59526918-59526937 Msa0778710:three_prime_UTR 40.0%
ACAAAATTGCCTCCATCTAC+AGG + chr5_2:59526996-59527015 Msa0778710:three_prime_UTR 40.0%
CATGTCATACTCCATGTGGA+AGG - chr5_2:59525997-59526016 None:intergenic 45.0%
AGCACATGTCATACTCCATG+TGG - chr5_2:59526001-59526020 None:intergenic 45.0%
CCTTCAAGGACGTGTTTCAA+TGG - chr5_2:59526105-59526124 None:intergenic 45.0%
CCATTGAAACACGTCCTTGA+AGG + chr5_2:59526102-59526121 Msa0778710:intron 45.0%
ACTAAGGCTCCAGCCAAATA+TGG - chr5_2:59526149-59526168 None:intergenic 45.0%
TGTTTGTGTAGCGTAAGGGA+AGG + chr5_2:59526280-59526299 Msa0778710:intron 45.0%
TTCCTGCTTCGTTTGCAAGA+GGG + chr5_2:59526304-59526323 Msa0778710:CDS 45.0%
! ACCTCAACCTCATTCTGGTT+CGG - chr5_2:59526369-59526388 None:intergenic 45.0%
! AGCTTTTGTGGGATTTTGGG+TGG + chr5_2:59526448-59526467 Msa0778710:three_prime_UTR 45.0%
GGTTAGAAATCTGCATGGAG+AGG + chr5_2:59526519-59526538 Msa0778710:three_prime_UTR 45.0%
ATCCTTGCTTCAATCCTCCA+CGG + chr5_2:59526551-59526570 Msa0778710:three_prime_UTR 45.0%
TTCTGCAACAACATTCTCCG+TGG - chr5_2:59526571-59526590 None:intergenic 45.0%
TGTCAAGGAGTTAACAGCTG+CGG + chr5_2:59526673-59526692 Msa0778710:three_prime_UTR 45.0%
!! GGACATTTCTTCTGGAGTGT+TGG + chr5_2:59526694-59526713 Msa0778710:three_prime_UTR 45.0%
TCTTTGTGCACCTCCAATGT+TGG + chr5_2:59526965-59526984 Msa0778710:three_prime_UTR 45.0%
GTCTTCAACACCAACATTGG+AGG - chr5_2:59526978-59526997 None:intergenic 45.0%
! TTTTCGCCAATGCCTGTAGA+TGG - chr5_2:59527011-59527030 None:intergenic 45.0%
!! TCTACAGGCATTGGCGAAAA+TGG + chr5_2:59527011-59527030 Msa0778710:three_prime_UTR 45.0%
!! GGGTTAGTTGCCTATTCAAG+TGG + chr5_2:59527113-59527132 Msa0778710:three_prime_UTR 45.0%
CATGTCATACTCCATGTGGA+AGG - chr5_2:59525997-59526016 None:intergenic 45.0%
AGCACATGTCATACTCCATG+TGG - chr5_2:59526001-59526020 None:intergenic 45.0%
CCTTCAAGGACGTGTTTCAA+TGG - chr5_2:59526105-59526124 None:intergenic 45.0%
CCATTGAAACACGTCCTTGA+AGG + chr5_2:59526102-59526121 Msa0778710:intron 45.0%
ACTAAGGCTCCAGCCAAATA+TGG - chr5_2:59526149-59526168 None:intergenic 45.0%
TGTTTGTGTAGCGTAAGGGA+AGG + chr5_2:59526280-59526299 Msa0778710:intron 45.0%
TTCCTGCTTCGTTTGCAAGA+GGG + chr5_2:59526304-59526323 Msa0778710:CDS 45.0%
! ACCTCAACCTCATTCTGGTT+CGG - chr5_2:59526369-59526388 None:intergenic 45.0%
! AGCTTTTGTGGGATTTTGGG+TGG + chr5_2:59526448-59526467 Msa0778710:three_prime_UTR 45.0%
GGTTAGAAATCTGCATGGAG+AGG + chr5_2:59526519-59526538 Msa0778710:three_prime_UTR 45.0%
ATCCTTGCTTCAATCCTCCA+CGG + chr5_2:59526551-59526570 Msa0778710:three_prime_UTR 45.0%
TTCTGCAACAACATTCTCCG+TGG - chr5_2:59526571-59526590 None:intergenic 45.0%
TGTCAAGGAGTTAACAGCTG+CGG + chr5_2:59526673-59526692 Msa0778710:three_prime_UTR 45.0%
!! GGACATTTCTTCTGGAGTGT+TGG + chr5_2:59526694-59526713 Msa0778710:three_prime_UTR 45.0%
TCTTTGTGCACCTCCAATGT+TGG + chr5_2:59526965-59526984 Msa0778710:three_prime_UTR 45.0%
GTCTTCAACACCAACATTGG+AGG - chr5_2:59526978-59526997 None:intergenic 45.0%
! TTTTCGCCAATGCCTGTAGA+TGG - chr5_2:59527011-59527030 None:intergenic 45.0%
!! TCTACAGGCATTGGCGAAAA+TGG + chr5_2:59527011-59527030 Msa0778710:three_prime_UTR 45.0%
!! GGGTTAGTTGCCTATTCAAG+TGG + chr5_2:59527113-59527132 Msa0778710:three_prime_UTR 45.0%
CTTCCTGCTTCGTTTGCAAG+AGG + chr5_2:59526303-59526322 Msa0778710:CDS 50.0%
AACCCTCTTGCAAACGAAGC+AGG - chr5_2:59526309-59526328 None:intergenic 50.0%
GCTTCGTTTGCAAGAGGGTT+TGG + chr5_2:59526309-59526328 Msa0778710:CDS 50.0%
ACGTGATCCGAACCAGAATG+AGG + chr5_2:59526359-59526378 Msa0778710:CDS 50.0%
TCCGAACCAGAATGAGGTTG+AGG + chr5_2:59526365-59526384 Msa0778710:CDS 50.0%
CAGCTACCTCAACCTCATTC+TGG - chr5_2:59526374-59526393 None:intergenic 50.0%
TGCAACAACATTCTCCGTGG+AGG - chr5_2:59526568-59526587 None:intergenic 50.0%
ACAGCTGCGGACATTTCTTC+TGG + chr5_2:59526686-59526705 Msa0778710:three_prime_UTR 50.0%
CACGGGAGCAGCTAACAATA+AGG + chr5_2:59526935-59526954 Msa0778710:three_prime_UTR 50.0%
TTGCCTCCATCTACAGGCAT+TGG + chr5_2:59527002-59527021 Msa0778710:three_prime_UTR 50.0%
CTTCCTGCTTCGTTTGCAAG+AGG + chr5_2:59526303-59526322 Msa0778710:CDS 50.0%
AACCCTCTTGCAAACGAAGC+AGG - chr5_2:59526309-59526328 None:intergenic 50.0%
GCTTCGTTTGCAAGAGGGTT+TGG + chr5_2:59526309-59526328 Msa0778710:CDS 50.0%
ACGTGATCCGAACCAGAATG+AGG + chr5_2:59526359-59526378 Msa0778710:CDS 50.0%
TCCGAACCAGAATGAGGTTG+AGG + chr5_2:59526365-59526384 Msa0778710:CDS 50.0%
CAGCTACCTCAACCTCATTC+TGG - chr5_2:59526374-59526393 None:intergenic 50.0%
TGCAACAACATTCTCCGTGG+AGG - chr5_2:59526568-59526587 None:intergenic 50.0%
ACAGCTGCGGACATTTCTTC+TGG + chr5_2:59526686-59526705 Msa0778710:three_prime_UTR 50.0%
CACGGGAGCAGCTAACAATA+AGG + chr5_2:59526935-59526954 Msa0778710:three_prime_UTR 50.0%
TTGCCTCCATCTACAGGCAT+TGG + chr5_2:59527002-59527021 Msa0778710:three_prime_UTR 50.0%
!! GTGGGATTTTGGGTGGTGCT+TGG + chr5_2:59526455-59526474 Msa0778710:three_prime_UTR 55.0%
CTCCGTGGAGGATTGAAGCA+AGG - chr5_2:59526556-59526575 None:intergenic 55.0%
! GTGCAACGCCCGTAAGTTGA+TGG + chr5_2:59526887-59526906 Msa0778710:three_prime_UTR 55.0%
TCGCCAATGCCTGTAGATGG+AGG - chr5_2:59527008-59527027 None:intergenic 55.0%
!! GTGGGATTTTGGGTGGTGCT+TGG + chr5_2:59526455-59526474 Msa0778710:three_prime_UTR 55.0%
CTCCGTGGAGGATTGAAGCA+AGG - chr5_2:59526556-59526575 None:intergenic 55.0%
! GTGCAACGCCCGTAAGTTGA+TGG + chr5_2:59526887-59526906 Msa0778710:three_prime_UTR 55.0%
TCGCCAATGCCTGTAGATGG+AGG - chr5_2:59527008-59527027 None:intergenic 55.0%
! TTACGGGCGTTGCACAGAGC+TGG - chr5_2:59526882-59526901 None:intergenic 60.0%
! TTACGGGCGTTGCACAGAGC+TGG - chr5_2:59526882-59526901 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 59525937 59527197 59525937 ID=Msa0778710;Name=Msa0778710
chr5_2 mRNA 59525937 59527197 59525937 ID=Msa0778710-mRNA-1;Parent=Msa0778710;Name=Msa0778710-mRNA-1;_AED=0.26;_eAED=0.26;_QI=65|1|0.5|1|1|0.5|2|769|52
chr5_2 exon 59525937 59526022 59525937 ID=Msa0778710-mRNA-1:exon:17470;Parent=Msa0778710-mRNA-1
chr5_2 exon 59526291 59527197 59526291 ID=Msa0778710-mRNA-1:exon:17471;Parent=Msa0778710-mRNA-1
chr5_2 five_prime_UTR 59525937 59526001 59525937 ID=Msa0778710-mRNA-1:five_prime_utr;Parent=Msa0778710-mRNA-1
chr5_2 CDS 59526002 59526022 59526002 ID=Msa0778710-mRNA-1:cds;Parent=Msa0778710-mRNA-1
chr5_2 CDS 59526291 59526428 59526291 ID=Msa0778710-mRNA-1:cds;Parent=Msa0778710-mRNA-1
chr5_2 three_prime_UTR 59526429 59527197 59526429 ID=Msa0778710-mRNA-1:three_prime_utr;Parent=Msa0778710-mRNA-1
Gene Sequence

>Msa0778710

ATGGAGTATGACATGTGCTCTCGTAAGGGAAGGCTTCCTGCTTCGTTTGCAAGAGGGTTTGGTCTACAAGTTGCTTCGTTTGTGTTGTTACGTGATCCGAACCAGAATGAGGTTGAGGTAGCTGTTGAAAAAGAAGAACGGGAAGGTTTATTTTACTGA
Protein sequence

>Msa0778710

MEYDMCSRKGRLPASFARGFGLQVASFVLLRDPNQNEVEVAVEKEEREGLFY*