AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0779270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779270 MtrunA17_Chr5g0435261 97.484 159 3 1 1 158 1 159 1.83e-114 320
Msa0779270 MtrunA17_Chr5g0435251 60.000 155 60 2 1 155 1 153 4.49e-59 180
Msa0779270 MtrunA17_Chr8g0350031 42.038 157 76 5 1 154 1 145 4.12e-35 119
Msa0779270 MtrunA17_Chr8g0350021 39.490 157 80 5 1 154 1 145 7.11e-32 111
Msa0779270 MtrunA17_Chr8g0348941 31.250 144 97 2 1 143 1 143 8.62e-19 79.0
Msa0779270 MtrunA17_Chr1g0147471 43.750 64 32 1 27 90 2 61 4.97e-16 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779270 AT3G55470.2 44.444 153 84 1 1 153 1 152 1.85e-43 141
Msa0779270 AT3G55470.1 44.156 154 84 2 1 153 1 153 1.24e-41 136
Msa0779270 AT1G63220.1 38.994 159 81 6 1 155 1 147 5.35e-27 99.4
Msa0779270 AT1G63220.2 35.220 159 73 6 1 155 1 133 3.86e-20 81.3
Msa0779270 AT4G00467.2 31.618 136 88 2 1 134 1 133 7.61e-20 82.0
Msa0779270 AT4G00467.1 31.618 136 88 2 1 134 1 133 1.49e-19 81.6
Msa0779270 AT4G34150.1 36.082 97 57 2 5 101 11 102 1.00e-12 63.9

Find 35 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTATTGTAAAGATTTAT+TGG 0.151708 5_2:+60440460 Msa0779270:CDS
TTCTTTCTAGCAAGTATTAA+AGG 0.194707 5_2:+60440875 Msa0779270:three_prime_UTR
CTCAATAGCACTTGTTTCTT+TGG 0.198258 5_2:+60440025 Msa0779270:CDS
GTAGCTTAGCAACTCCATTT+TGG 0.254845 5_2:-60440493 None:intergenic
TTGGCTCAAGGTGTCCAAAA+TGG 0.321323 5_2:+60440479 Msa0779270:CDS
AGATATGGCAATTGGGATAA+TGG 0.338735 5_2:+60439976 Msa0779270:exon
CAAAGTCATTCTCAAAATAA+TGG 0.344213 5_2:+60440285 Msa0779270:CDS
GGCTCCTATGCTCTTGGCCT+TGG 0.379325 5_2:-60440177 None:intergenic
ACACTCAAAGATATGGCAAT+TGG 0.382620 5_2:+60439968 Msa0779270:exon
GTAGAGGAAGAAATCATGTA+TGG 0.444098 5_2:+60440212 Msa0779270:CDS
TTCTCATACACTCAAAGATA+TGG 0.452127 5_2:+60439961 Msa0779270:exon
CACTCAAAGATATGGCAATT+GGG 0.464148 5_2:+60439969 Msa0779270:exon
CATAAATGTATGTTGTTACA+TGG 0.468329 5_2:+60440898 Msa0779270:three_prime_UTR
CAATCTCTCCACGATAAGAT+TGG 0.475600 5_2:-60440547 None:intergenic
CAAAGAAACAAGTGCTATTG+AGG 0.483426 5_2:-60440024 None:intergenic
CTATATTAATATAGGTGGAA+TGG 0.484803 5_2:+60440129 Msa0779270:intron
CCACACGGCTCCTATGCTCT+TGG 0.487013 5_2:-60440183 None:intergenic
CATAGGAGCCGTGTGGCTAA+AGG 0.495986 5_2:+60440190 Msa0779270:CDS
TTGGTATTGTATCAAAACAT+AGG 0.496228 5_2:-60440158 None:intergenic
TGTAAAGATTTATTGGCTCA+AGG 0.515900 5_2:+60440467 Msa0779270:CDS
AATTCATTCACAGGTGGAAG+AGG 0.517863 5_2:+60440705 Msa0779270:intron
AGCCGTGTGGCTAAAGGTAG+AGG 0.518837 5_2:+60440196 Msa0779270:CDS
TTCCTCTACCTTTAGCCACA+CGG 0.519437 5_2:-60440198 None:intergenic
GTACATGTTGTGAAAGCTAA+AGG 0.526255 5_2:+60440001 Msa0779270:CDS
TTGAAGAAGACATAGGAGGA+TGG 0.550578 5_2:+60440734 Msa0779270:CDS
TGGTATTGTATCAAAACATA+GGG 0.550960 5_2:-60440157 None:intergenic
GAATTAATTGAAGAAGACAT+AGG 0.564791 5_2:+60440727 Msa0779270:CDS
AATACCAAGGCCAAGAGCAT+AGG 0.592231 5_2:+60440173 Msa0779270:CDS
TAGAGGAAGAAATCATGTAT+GGG 0.596604 5_2:+60440213 Msa0779270:CDS
CGAACAACTCTATACTTGAG+AGG 0.601631 5_2:-60440518 None:intergenic
TTAATTGAAGAAGACATAGG+AGG 0.621683 5_2:+60440730 Msa0779270:CDS
ATGTTCAAAGTAGAAAACAA+TGG 0.628110 5_2:+60440247 Msa0779270:CDS
CGTGCAGACCAATCTTATCG+TGG 0.690565 5_2:+60440539 Msa0779270:CDS
ACAACTCTATACTTGAGAGG+TGG 0.693150 5_2:-60440515 None:intergenic
CCAAGAGCATAGGAGCCGTG+TGG 0.728413 5_2:+60440183 Msa0779270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAATTAATTACCTT+TGG - chr5_2:60440593-60440612 None:intergenic 10.0%
!! ATATATTAATTAATTACCTT+TGG - chr5_2:60440593-60440612 None:intergenic 10.0%
!!! TTTTTTGAATTTGAATCTTT+AGG + chr5_2:60439922-60439941 Msa0779270:five_prime_UTR 15.0%
!!! TTTTTGAATTTGAATCTTTA+GGG + chr5_2:60439923-60439942 Msa0779270:five_prime_UTR 15.0%
!!! TTTTACTATATTAATATAGG+TGG + chr5_2:60440124-60440143 Msa0779270:intron 15.0%
!! AATCTATTGTAAAGATTTAT+TGG + chr5_2:60440460-60440479 Msa0779270:CDS 15.0%
!!! TTCATATTTCATTTTTGTTA+AGG + chr5_2:60440612-60440631 Msa0779270:intron 15.0%
!!! TTTTTTGAATTTGAATCTTT+AGG + chr5_2:60439922-60439941 Msa0779270:five_prime_UTR 15.0%
!!! TTTTTGAATTTGAATCTTTA+GGG + chr5_2:60439923-60439942 Msa0779270:five_prime_UTR 15.0%
!!! TTTTACTATATTAATATAGG+TGG + chr5_2:60440124-60440143 Msa0779270:intron 15.0%
!! AATCTATTGTAAAGATTTAT+TGG + chr5_2:60440460-60440479 Msa0779270:CDS 15.0%
!!! TTCATATTTCATTTTTGTTA+AGG + chr5_2:60440612-60440631 Msa0779270:intron 15.0%
!! TATGATATGATATGATACAA+GGG - chr5_2:60440091-60440110 None:intergenic 20.0%
!! ATATGATATGATATGATACA+AGG - chr5_2:60440092-60440111 None:intergenic 20.0%
!! TATTGTTAGGAATATATATG+TGG - chr5_2:60440370-60440389 None:intergenic 20.0%
!! AAAAATGAGACATTATTGTT+AGG - chr5_2:60440383-60440402 None:intergenic 20.0%
!! TTTACAATAGATTCTGTTTA+AGG - chr5_2:60440453-60440472 None:intergenic 20.0%
!! CATGTAACAATATATAGTTA+TGG + chr5_2:60440635-60440654 Msa0779270:intron 20.0%
!!! AGTTGATTATTTTTCTTTGT+TGG + chr5_2:60440682-60440701 Msa0779270:intron 20.0%
!!! ACTACTTTTAGTTTACAAAT+GGG - chr5_2:60440802-60440821 None:intergenic 20.0%
!!! AACTACTTTTAGTTTACAAA+TGG - chr5_2:60440803-60440822 None:intergenic 20.0%
!! TATGATATGATATGATACAA+GGG - chr5_2:60440091-60440110 None:intergenic 20.0%
!! ATATGATATGATATGATACA+AGG - chr5_2:60440092-60440111 None:intergenic 20.0%
!! TATTGTTAGGAATATATATG+TGG - chr5_2:60440370-60440389 None:intergenic 20.0%
!! AAAAATGAGACATTATTGTT+AGG - chr5_2:60440383-60440402 None:intergenic 20.0%
!! TTTACAATAGATTCTGTTTA+AGG - chr5_2:60440453-60440472 None:intergenic 20.0%
!! CATGTAACAATATATAGTTA+TGG + chr5_2:60440635-60440654 Msa0779270:intron 20.0%
!!! AGTTGATTATTTTTCTTTGT+TGG + chr5_2:60440682-60440701 Msa0779270:intron 20.0%
!!! ACTACTTTTAGTTTACAAAT+GGG - chr5_2:60440802-60440821 None:intergenic 20.0%
!!! AACTACTTTTAGTTTACAAA+TGG - chr5_2:60440803-60440822 None:intergenic 20.0%
! GGCAAAATATATGAATGTAA+GGG - chr5_2:60440070-60440089 None:intergenic 25.0%
! CTATATTAATATAGGTGGAA+TGG + chr5_2:60440129-60440148 Msa0779270:intron 25.0%
! TGGTATTGTATCAAAACATA+GGG - chr5_2:60440160-60440179 None:intergenic 25.0%
! TTGGTATTGTATCAAAACAT+AGG - chr5_2:60440161-60440180 None:intergenic 25.0%
!!! TATGTTTTGATACAATACCA+AGG + chr5_2:60440160-60440179 Msa0779270:CDS 25.0%
! ATGTTCAAAGTAGAAAACAA+TGG + chr5_2:60440247-60440266 Msa0779270:CDS 25.0%
! CAAAGTCATTCTCAAAATAA+TGG + chr5_2:60440285-60440304 Msa0779270:CDS 25.0%
! GAATTAATTGAAGAAGACAT+AGG + chr5_2:60440727-60440746 Msa0779270:CDS 25.0%
!! TTCTTTCTAGCAAGTATTAA+AGG + chr5_2:60440875-60440894 Msa0779270:three_prime_UTR 25.0%
! CATAAATGTATGTTGTTACA+TGG + chr5_2:60440898-60440917 Msa0779270:three_prime_UTR 25.0%
! GGCAAAATATATGAATGTAA+GGG - chr5_2:60440070-60440089 None:intergenic 25.0%
! CTATATTAATATAGGTGGAA+TGG + chr5_2:60440129-60440148 Msa0779270:intron 25.0%
! TGGTATTGTATCAAAACATA+GGG - chr5_2:60440160-60440179 None:intergenic 25.0%
! TTGGTATTGTATCAAAACAT+AGG - chr5_2:60440161-60440180 None:intergenic 25.0%
!!! TATGTTTTGATACAATACCA+AGG + chr5_2:60440160-60440179 Msa0779270:CDS 25.0%
! ATGTTCAAAGTAGAAAACAA+TGG + chr5_2:60440247-60440266 Msa0779270:CDS 25.0%
! CAAAGTCATTCTCAAAATAA+TGG + chr5_2:60440285-60440304 Msa0779270:CDS 25.0%
! GAATTAATTGAAGAAGACAT+AGG + chr5_2:60440727-60440746 Msa0779270:CDS 25.0%
!! TTCTTTCTAGCAAGTATTAA+AGG + chr5_2:60440875-60440894 Msa0779270:three_prime_UTR 25.0%
! CATAAATGTATGTTGTTACA+TGG + chr5_2:60440898-60440917 Msa0779270:three_prime_UTR 25.0%
TTCTCATACACTCAAAGATA+TGG + chr5_2:60439961-60439980 Msa0779270:exon 30.0%
GGGCAAAATATATGAATGTA+AGG - chr5_2:60440071-60440090 None:intergenic 30.0%
TAGAGGAAGAAATCATGTAT+GGG + chr5_2:60440213-60440232 Msa0779270:CDS 30.0%
GTTAGGAATATATATGTGGA+AGG - chr5_2:60440366-60440385 None:intergenic 30.0%
!! TGTAAAGATTTATTGGCTCA+AGG + chr5_2:60440467-60440486 Msa0779270:CDS 30.0%
!! TGCTATTACTTTTACTCCAA+AGG + chr5_2:60440574-60440593 Msa0779270:CDS 30.0%
TTAATTGAAGAAGACATAGG+AGG + chr5_2:60440730-60440749 Msa0779270:CDS 30.0%
TTCTCATACACTCAAAGATA+TGG + chr5_2:60439961-60439980 Msa0779270:exon 30.0%
GGGCAAAATATATGAATGTA+AGG - chr5_2:60440071-60440090 None:intergenic 30.0%
TAGAGGAAGAAATCATGTAT+GGG + chr5_2:60440213-60440232 Msa0779270:CDS 30.0%
GTTAGGAATATATATGTGGA+AGG - chr5_2:60440366-60440385 None:intergenic 30.0%
!! TGTAAAGATTTATTGGCTCA+AGG + chr5_2:60440467-60440486 Msa0779270:CDS 30.0%
!! TGCTATTACTTTTACTCCAA+AGG + chr5_2:60440574-60440593 Msa0779270:CDS 30.0%
TTAATTGAAGAAGACATAGG+AGG + chr5_2:60440730-60440749 Msa0779270:CDS 30.0%
ACACTCAAAGATATGGCAAT+TGG + chr5_2:60439968-60439987 Msa0779270:exon 35.0%
CACTCAAAGATATGGCAATT+GGG + chr5_2:60439969-60439988 Msa0779270:exon 35.0%
AGATATGGCAATTGGGATAA+TGG + chr5_2:60439976-60439995 Msa0779270:exon 35.0%
GTACATGTTGTGAAAGCTAA+AGG + chr5_2:60440001-60440020 Msa0779270:CDS 35.0%
! CAAAGAAACAAGTGCTATTG+AGG - chr5_2:60440027-60440046 None:intergenic 35.0%
! CTCAATAGCACTTGTTTCTT+TGG + chr5_2:60440025-60440044 Msa0779270:CDS 35.0%
GTAGAGGAAGAAATCATGTA+TGG + chr5_2:60440212-60440231 Msa0779270:CDS 35.0%
! TTTTCTGCTGATGACTTCAT+TGG + chr5_2:60440319-60440338 Msa0779270:CDS 35.0%
CTTTGTTGGAATTCATTCAC+AGG + chr5_2:60440696-60440715 Msa0779270:intron 35.0%
! AAAAACAACAAGCATCGTAG+AGG - chr5_2:60440926-60440945 None:intergenic 35.0%
ACACTCAAAGATATGGCAAT+TGG + chr5_2:60439968-60439987 Msa0779270:exon 35.0%
CACTCAAAGATATGGCAATT+GGG + chr5_2:60439969-60439988 Msa0779270:exon 35.0%
AGATATGGCAATTGGGATAA+TGG + chr5_2:60439976-60439995 Msa0779270:exon 35.0%
GTACATGTTGTGAAAGCTAA+AGG + chr5_2:60440001-60440020 Msa0779270:CDS 35.0%
! CAAAGAAACAAGTGCTATTG+AGG - chr5_2:60440027-60440046 None:intergenic 35.0%
! CTCAATAGCACTTGTTTCTT+TGG + chr5_2:60440025-60440044 Msa0779270:CDS 35.0%
GTAGAGGAAGAAATCATGTA+TGG + chr5_2:60440212-60440231 Msa0779270:CDS 35.0%
! TTTTCTGCTGATGACTTCAT+TGG + chr5_2:60440319-60440338 Msa0779270:CDS 35.0%
CTTTGTTGGAATTCATTCAC+AGG + chr5_2:60440696-60440715 Msa0779270:intron 35.0%
! AAAAACAACAAGCATCGTAG+AGG - chr5_2:60440926-60440945 None:intergenic 35.0%
! GTAGCTTAGCAACTCCATTT+TGG - chr5_2:60440496-60440515 None:intergenic 40.0%
ACAACTCTATACTTGAGAGG+TGG - chr5_2:60440518-60440537 None:intergenic 40.0%
CGAACAACTCTATACTTGAG+AGG - chr5_2:60440521-60440540 None:intergenic 40.0%
CAATCTCTCCACGATAAGAT+TGG - chr5_2:60440550-60440569 None:intergenic 40.0%
TGTTGGAATTCATTCACAGG+TGG + chr5_2:60440699-60440718 Msa0779270:intron 40.0%
AATTCATTCACAGGTGGAAG+AGG + chr5_2:60440705-60440724 Msa0779270:intron 40.0%
TTGAAGAAGACATAGGAGGA+TGG + chr5_2:60440734-60440753 Msa0779270:CDS 40.0%
! GTAGCTTAGCAACTCCATTT+TGG - chr5_2:60440496-60440515 None:intergenic 40.0%
ACAACTCTATACTTGAGAGG+TGG - chr5_2:60440518-60440537 None:intergenic 40.0%
CGAACAACTCTATACTTGAG+AGG - chr5_2:60440521-60440540 None:intergenic 40.0%
CAATCTCTCCACGATAAGAT+TGG - chr5_2:60440550-60440569 None:intergenic 40.0%
TGTTGGAATTCATTCACAGG+TGG + chr5_2:60440699-60440718 Msa0779270:intron 40.0%
AATTCATTCACAGGTGGAAG+AGG + chr5_2:60440705-60440724 Msa0779270:intron 40.0%
TTGAAGAAGACATAGGAGGA+TGG + chr5_2:60440734-60440753 Msa0779270:CDS 40.0%
! AATACCAAGGCCAAGAGCAT+AGG + chr5_2:60440173-60440192 Msa0779270:CDS 45.0%
TTCCTCTACCTTTAGCCACA+CGG - chr5_2:60440201-60440220 None:intergenic 45.0%
!! TGGAAGGCATTAGTCTTACG+TGG - chr5_2:60440350-60440369 None:intergenic 45.0%
! TTGGCTCAAGGTGTCCAAAA+TGG + chr5_2:60440479-60440498 Msa0779270:CDS 45.0%
! AATACCAAGGCCAAGAGCAT+AGG + chr5_2:60440173-60440192 Msa0779270:CDS 45.0%
TTCCTCTACCTTTAGCCACA+CGG - chr5_2:60440201-60440220 None:intergenic 45.0%
!! TGGAAGGCATTAGTCTTACG+TGG - chr5_2:60440350-60440369 None:intergenic 45.0%
! TTGGCTCAAGGTGTCCAAAA+TGG + chr5_2:60440479-60440498 Msa0779270:CDS 45.0%
!!! TATTTTTACTATATTAATAT+AGG + chr5_2:60440121-60440140 Msa0779270:intron 5.0%
!!! TATTTTTACTATATTAATAT+AGG + chr5_2:60440121-60440140 Msa0779270:intron 5.0%
CGTGCAGACCAATCTTATCG+TGG + chr5_2:60440539-60440558 Msa0779270:CDS 50.0%
CGTGCAGACCAATCTTATCG+TGG + chr5_2:60440539-60440558 Msa0779270:CDS 50.0%
CATAGGAGCCGTGTGGCTAA+AGG + chr5_2:60440190-60440209 Msa0779270:CDS 55.0%
AGCCGTGTGGCTAAAGGTAG+AGG + chr5_2:60440196-60440215 Msa0779270:CDS 55.0%
CATAGGAGCCGTGTGGCTAA+AGG + chr5_2:60440190-60440209 Msa0779270:CDS 55.0%
AGCCGTGTGGCTAAAGGTAG+AGG + chr5_2:60440196-60440215 Msa0779270:CDS 55.0%
GGCTCCTATGCTCTTGGCCT+TGG - chr5_2:60440180-60440199 None:intergenic 60.0%
CCACACGGCTCCTATGCTCT+TGG - chr5_2:60440186-60440205 None:intergenic 60.0%
! CCAAGAGCATAGGAGCCGTG+TGG + chr5_2:60440183-60440202 Msa0779270:CDS 60.0%
GGCTCCTATGCTCTTGGCCT+TGG - chr5_2:60440180-60440199 None:intergenic 60.0%
CCACACGGCTCCTATGCTCT+TGG - chr5_2:60440186-60440205 None:intergenic 60.0%
! CCAAGAGCATAGGAGCCGTG+TGG + chr5_2:60440183-60440202 Msa0779270:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 60439894 60440992 60439894 ID=Msa0779270;Name=Msa0779270
chr5_2 mRNA 60439894 60440992 60439894 ID=Msa0779270-mRNA-1;Parent=Msa0779270;Name=Msa0779270-mRNA-1;_AED=0.30;_eAED=0.30;_QI=86|1|1|1|1|1|4|209|158
chr5_2 exon 60439894 60440046 60439894 ID=Msa0779270-mRNA-1:exon:17726;Parent=Msa0779270-mRNA-1
chr5_2 exon 60440143 60440350 60440143 ID=Msa0779270-mRNA-1:exon:17727;Parent=Msa0779270-mRNA-1
chr5_2 exon 60440460 60440595 60440460 ID=Msa0779270-mRNA-1:exon:17728;Parent=Msa0779270-mRNA-1
chr5_2 exon 60440718 60440992 60440718 ID=Msa0779270-mRNA-1:exon:17729;Parent=Msa0779270-mRNA-1
chr5_2 five_prime_UTR 60439894 60439979 60439894 ID=Msa0779270-mRNA-1:five_prime_utr;Parent=Msa0779270-mRNA-1
chr5_2 CDS 60439980 60440046 60439980 ID=Msa0779270-mRNA-1:cds;Parent=Msa0779270-mRNA-1
chr5_2 CDS 60440143 60440350 60440143 ID=Msa0779270-mRNA-1:cds;Parent=Msa0779270-mRNA-1
chr5_2 CDS 60440460 60440595 60440460 ID=Msa0779270-mRNA-1:cds;Parent=Msa0779270-mRNA-1
chr5_2 CDS 60440718 60440783 60440718 ID=Msa0779270-mRNA-1:cds;Parent=Msa0779270-mRNA-1
chr5_2 three_prime_UTR 60440784 60440992 60440784 ID=Msa0779270-mRNA-1:three_prime_utr;Parent=Msa0779270-mRNA-1
Gene Sequence

>Msa0779270

ATGGCAATTGGGATAATGGAAGTACATGTTGTGAAAGCTAAAGGCCTCAATAGCACTTGTTTCTTTGGTGGAATGGAAGATCCCTATGTTTTGATACAATACCAAGGCCAAGAGCATAGGAGCCGTGTGGCTAAAGGTAGAGGAAGAAATCATGTATGGGATGAGATATTTATGTTCAAAGTAGAAAACAATGGATCAAATGATAAAAACAAAGTCATTCTCAAAATAATGGACAAAGATAGCTTTTCTGCTGATGACTTCATTGGACAAGCCACAATCTATTGTAAAGATTTATTGGCTCAAGGTGTCCAAAATGGAGTTGCTAAGCTACCACCTCTCAAGTATAGAGTTGTTCGTGCAGACCAATCTTATCGTGGAGAGATTGAAGTTGCTATTACTTTTACTCCAAAGGTGGAAGAGGAATTAATTGAAGAAGACATAGGAGGATGGAAAGAAAGTAGTTACTATATATCTTAA

Protein sequence

>Msa0779270

MAIGIMEVHVVKAKGLNSTCFFGGMEDPYVLIQYQGQEHRSRVAKGRGRNHVWDEIFMFKVENNGSNDKNKVILKIMDKDSFSADDFIGQATIYCKDLLAQGVQNGVAKLPPLKYRVVRADQSYRGEIEVAITFTPKVEEELIEEDIGGWKESSYYIS*