AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0737500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737500 MtrunA17_Chr5g0435261 97.484 159 3 1 1 158 1 159 3.77e-114 320
Msa0737500 MtrunA17_Chr5g0435251 60.645 155 59 2 1 155 1 153 1.56e-59 181
Msa0737500 MtrunA17_Chr8g0350031 42.038 157 76 5 1 154 1 145 4.70e-35 119
Msa0737500 MtrunA17_Chr8g0350021 40.127 157 79 5 1 154 1 145 1.15e-32 113
Msa0737500 MtrunA17_Chr8g0348941 31.944 144 96 2 1 143 1 143 4.71e-19 79.3
Msa0737500 MtrunA17_Chr1g0147471 43.750 64 32 1 27 90 2 61 3.33e-15 66.2
Msa0737500 MtrunA17_Chr2g0290721 29.365 126 82 3 24 149 20 138 5.62e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737500 AT3G55470.2 45.098 153 83 1 1 153 1 152 6.01e-44 142
Msa0737500 AT3G55470.1 44.805 154 83 2 1 153 1 153 4.16e-42 138
Msa0737500 AT1G63220.1 39.623 159 80 6 1 155 1 147 8.05e-28 101
Msa0737500 AT1G63220.2 35.849 159 72 6 1 155 1 133 5.51e-21 83.6
Msa0737500 AT4G00467.2 32.353 136 87 2 1 134 1 133 7.06e-20 82.0
Msa0737500 AT4G00467.1 32.353 136 87 2 1 134 1 133 1.08e-19 82.0
Msa0737500 AT4G34150.1 34.694 98 57 3 5 101 11 102 1.33e-12 63.9

Find 35 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTATTGTAAAGATTTAT+TGG 0.151708 5_1:+54745475 Msa0737500:CDS
CTCAATAACACTTGTTTCTT+TGG 0.179953 5_1:+54745041 Msa0737500:CDS
TTCTTTCTAGCAAGCATTAA+AGG 0.192764 5_1:+54745887 Msa0737500:three_prime_UTR
TTGGCTCAAGGTGTTCAAAA+TGG 0.301303 5_1:+54745494 Msa0737500:CDS
AGATATGGCAATTGGGATAA+TGG 0.328865 5_1:+54744992 Msa0737500:exon
CAAAGTCATTCTCAAAATAA+TGG 0.344213 5_1:+54745300 Msa0737500:CDS
ACACTTAAAGATATGGCAAT+TGG 0.360757 5_1:+54744984 Msa0737500:exon
TTCTCATACACTTAAAGATA+TGG 0.438648 5_1:+54744977 Msa0737500:exon
CACTTAAAGATATGGCAATT+GGG 0.452310 5_1:+54744985 Msa0737500:exon
GCATAGGAGCCGTGTGGCTA+AGG 0.463549 5_1:+54745204 Msa0737500:CDS
CATAAATGTATGTTGTTACA+TGG 0.468329 5_1:+54745910 Msa0737500:three_prime_UTR
CAATCTCTCCACGATAAGAT+TGG 0.475600 5_1:-54745562 None:intergenic
GTACATCTTGTGAAAGCAAA+AGG 0.483814 5_1:+54745017 Msa0737500:CDS
TTGGTATTGTATCAAAACAT+AGG 0.499452 5_1:-54745173 None:intergenic
GTAGAGGCAGAAATCATGTA+TGG 0.500046 5_1:+54745227 Msa0737500:CDS
AGCCGTGTGGCTAAGGGTAG+AGG 0.500839 5_1:+54745211 Msa0737500:CDS
CAAAGAAACAAGTGTTATTG+AGG 0.514718 5_1:-54745040 None:intergenic
TGTAAAGATTTATTGGCTCA+AGG 0.515900 5_1:+54745482 Msa0737500:CDS
AATTCATTCACAGGTGGAAG+AGG 0.517863 5_1:+54745720 Msa0737500:intron
GGCTCCTATGCTCTTGACCT+TGG 0.540922 5_1:-54745192 None:intergenic
ATATTAATATATAGGTGGAA+TGG 0.546882 5_1:+54745144 Msa0737500:intron
TGGTATTGTATCAAAACATA+GGG 0.548025 5_1:-54745172 None:intergenic
TTGAAGAAGACATAGGAGGA+TGG 0.550578 5_1:+54745749 Msa0737500:CDS
CATAGGAGCCGTGTGGCTAA+GGG 0.562336 5_1:+54745205 Msa0737500:CDS
GAATTAATTGAAGAAGACAT+AGG 0.564791 5_1:+54745742 Msa0737500:CDS
ACAGAAAACAAGCATCATAG+AGG 0.582943 5_1:-54745935 None:intergenic
ATGTTCAAAGTAGAAAACAT+TGG 0.586845 5_1:+54745262 Msa0737500:CDS
AATACCAAGGTCAAGAGCAT+AGG 0.590229 5_1:+54745188 Msa0737500:CDS
TGCCTCTACCCTTAGCCACA+CGG 0.590713 5_1:-54745213 None:intergenic
CGAACAACTCTATACTTGAG+AGG 0.601631 5_1:-54745533 None:intergenic
TTAATTGAAGAAGACATAGG+AGG 0.621683 5_1:+54745745 Msa0737500:CDS
TAGAGGCAGAAATCATGTAT+GGG 0.623868 5_1:+54745228 Msa0737500:CDS
CGTGCAGACCAATCTTATCG+TGG 0.690565 5_1:+54745554 Msa0737500:CDS
ACAACTCTATACTTGAGAGG+TGG 0.693150 5_1:-54745530 None:intergenic
TCAAGAGCATAGGAGCCGTG+TGG 0.747555 5_1:+54745198 Msa0737500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAATTAATTACCTT+TGG - chr5_1:54745608-54745627 None:intergenic 10.0%
!! ATATATTAATTAATTACCTT+TGG - chr5_1:54745608-54745627 None:intergenic 10.0%
!!! TTTTTTGAATTTAAATCTTC+AGG + chr5_1:54744938-54744957 Msa0737500:five_prime_UTR 15.0%
!!! TTTTTGAATTTAAATCTTCA+GGG + chr5_1:54744939-54744958 Msa0737500:five_prime_UTR 15.0%
!! TTACTATATTAATATATAGG+TGG + chr5_1:54745139-54745158 Msa0737500:intron 15.0%
!! AATCTATTGTAAAGATTTAT+TGG + chr5_1:54745475-54745494 Msa0737500:CDS 15.0%
!!! TTCACATTTTATTTTTGTTA+AGG + chr5_1:54745627-54745646 Msa0737500:intron 15.0%
!!! TTTTTTGAATTTAAATCTTC+AGG + chr5_1:54744938-54744957 Msa0737500:five_prime_UTR 15.0%
!!! TTTTTGAATTTAAATCTTCA+GGG + chr5_1:54744939-54744958 Msa0737500:five_prime_UTR 15.0%
!! TTACTATATTAATATATAGG+TGG + chr5_1:54745139-54745158 Msa0737500:intron 15.0%
!! AATCTATTGTAAAGATTTAT+TGG + chr5_1:54745475-54745494 Msa0737500:CDS 15.0%
!!! TTCACATTTTATTTTTGTTA+AGG + chr5_1:54745627-54745646 Msa0737500:intron 15.0%
!! TATGATATGATATGATACAA+GGG - chr5_1:54745107-54745126 None:intergenic 20.0%
!! ATATGATATGATATGATACA+AGG - chr5_1:54745108-54745127 None:intergenic 20.0%
!! ATATTAATATATAGGTGGAA+TGG + chr5_1:54745144-54745163 Msa0737500:intron 20.0%
!! AAAAATGAGACATTATTGTT+AGG - chr5_1:54745398-54745417 None:intergenic 20.0%
!! TTTACAATAGATTCTGTTTA+AGG - chr5_1:54745468-54745487 None:intergenic 20.0%
!! CATGTAACAATATATAGTTA+TGG + chr5_1:54745650-54745669 Msa0737500:intron 20.0%
!!! AGTTGATTATTTTTCTTTGT+TGG + chr5_1:54745697-54745716 Msa0737500:intron 20.0%
!!! ACTACTTTTAGTTTACAAAT+GGG - chr5_1:54745817-54745836 None:intergenic 20.0%
!!! AACTACTTTTAGTTTACAAA+TGG - chr5_1:54745818-54745837 None:intergenic 20.0%
!! TATGATATGATATGATACAA+GGG - chr5_1:54745107-54745126 None:intergenic 20.0%
!! ATATGATATGATATGATACA+AGG - chr5_1:54745108-54745127 None:intergenic 20.0%
!! ATATTAATATATAGGTGGAA+TGG + chr5_1:54745144-54745163 Msa0737500:intron 20.0%
!! AAAAATGAGACATTATTGTT+AGG - chr5_1:54745398-54745417 None:intergenic 20.0%
!! TTTACAATAGATTCTGTTTA+AGG - chr5_1:54745468-54745487 None:intergenic 20.0%
!! CATGTAACAATATATAGTTA+TGG + chr5_1:54745650-54745669 Msa0737500:intron 20.0%
!!! AGTTGATTATTTTTCTTTGT+TGG + chr5_1:54745697-54745716 Msa0737500:intron 20.0%
!!! ACTACTTTTAGTTTACAAAT+GGG - chr5_1:54745817-54745836 None:intergenic 20.0%
!!! AACTACTTTTAGTTTACAAA+TGG - chr5_1:54745818-54745837 None:intergenic 20.0%
! TTCTCATACACTTAAAGATA+TGG + chr5_1:54744977-54744996 Msa0737500:exon 25.0%
! GGCAAAATATATGAATGTAA+GGG - chr5_1:54745086-54745105 None:intergenic 25.0%
! TGGTATTGTATCAAAACATA+GGG - chr5_1:54745175-54745194 None:intergenic 25.0%
! TTGGTATTGTATCAAAACAT+AGG - chr5_1:54745176-54745195 None:intergenic 25.0%
!!! TATGTTTTGATACAATACCA+AGG + chr5_1:54745175-54745194 Msa0737500:CDS 25.0%
! ATGTTCAAAGTAGAAAACAT+TGG + chr5_1:54745262-54745281 Msa0737500:CDS 25.0%
! CAAAGTCATTCTCAAAATAA+TGG + chr5_1:54745300-54745319 Msa0737500:CDS 25.0%
!! TATTGTTAGGAGTATATATG+TGG - chr5_1:54745385-54745404 None:intergenic 25.0%
! GAATTAATTGAAGAAGACAT+AGG + chr5_1:54745742-54745761 Msa0737500:CDS 25.0%
! CATAAATGTATGTTGTTACA+TGG + chr5_1:54745910-54745929 Msa0737500:three_prime_UTR 25.0%
! TTCTCATACACTTAAAGATA+TGG + chr5_1:54744977-54744996 Msa0737500:exon 25.0%
! GGCAAAATATATGAATGTAA+GGG - chr5_1:54745086-54745105 None:intergenic 25.0%
! TGGTATTGTATCAAAACATA+GGG - chr5_1:54745175-54745194 None:intergenic 25.0%
! TTGGTATTGTATCAAAACAT+AGG - chr5_1:54745176-54745195 None:intergenic 25.0%
!!! TATGTTTTGATACAATACCA+AGG + chr5_1:54745175-54745194 Msa0737500:CDS 25.0%
! ATGTTCAAAGTAGAAAACAT+TGG + chr5_1:54745262-54745281 Msa0737500:CDS 25.0%
! CAAAGTCATTCTCAAAATAA+TGG + chr5_1:54745300-54745319 Msa0737500:CDS 25.0%
!! TATTGTTAGGAGTATATATG+TGG - chr5_1:54745385-54745404 None:intergenic 25.0%
! GAATTAATTGAAGAAGACAT+AGG + chr5_1:54745742-54745761 Msa0737500:CDS 25.0%
! CATAAATGTATGTTGTTACA+TGG + chr5_1:54745910-54745929 Msa0737500:three_prime_UTR 25.0%
ACACTTAAAGATATGGCAAT+TGG + chr5_1:54744984-54745003 Msa0737500:exon 30.0%
CACTTAAAGATATGGCAATT+GGG + chr5_1:54744985-54745004 Msa0737500:exon 30.0%
! CAAAGAAACAAGTGTTATTG+AGG - chr5_1:54745043-54745062 None:intergenic 30.0%
! CTCAATAACACTTGTTTCTT+TGG + chr5_1:54745041-54745060 Msa0737500:CDS 30.0%
GGGCAAAATATATGAATGTA+AGG - chr5_1:54745087-54745106 None:intergenic 30.0%
!! TGTAAAGATTTATTGGCTCA+AGG + chr5_1:54745482-54745501 Msa0737500:CDS 30.0%
!! TGCTATTACTTTTACTCCAA+AGG + chr5_1:54745589-54745608 Msa0737500:CDS 30.0%
TTAATTGAAGAAGACATAGG+AGG + chr5_1:54745745-54745764 Msa0737500:CDS 30.0%
! TTCTTTCTAGCAAGCATTAA+AGG + chr5_1:54745887-54745906 Msa0737500:three_prime_UTR 30.0%
ACACTTAAAGATATGGCAAT+TGG + chr5_1:54744984-54745003 Msa0737500:exon 30.0%
CACTTAAAGATATGGCAATT+GGG + chr5_1:54744985-54745004 Msa0737500:exon 30.0%
! CAAAGAAACAAGTGTTATTG+AGG - chr5_1:54745043-54745062 None:intergenic 30.0%
! CTCAATAACACTTGTTTCTT+TGG + chr5_1:54745041-54745060 Msa0737500:CDS 30.0%
GGGCAAAATATATGAATGTA+AGG - chr5_1:54745087-54745106 None:intergenic 30.0%
!! TGTAAAGATTTATTGGCTCA+AGG + chr5_1:54745482-54745501 Msa0737500:CDS 30.0%
!! TGCTATTACTTTTACTCCAA+AGG + chr5_1:54745589-54745608 Msa0737500:CDS 30.0%
TTAATTGAAGAAGACATAGG+AGG + chr5_1:54745745-54745764 Msa0737500:CDS 30.0%
! TTCTTTCTAGCAAGCATTAA+AGG + chr5_1:54745887-54745906 Msa0737500:three_prime_UTR 30.0%
AGATATGGCAATTGGGATAA+TGG + chr5_1:54744992-54745011 Msa0737500:exon 35.0%
GTACATCTTGTGAAAGCAAA+AGG + chr5_1:54745017-54745036 Msa0737500:CDS 35.0%
TAGAGGCAGAAATCATGTAT+GGG + chr5_1:54745228-54745247 Msa0737500:CDS 35.0%
! TTTTCTGCTGATGACTTCAT+TGG + chr5_1:54745334-54745353 Msa0737500:CDS 35.0%
! GTTAGGAGTATATATGTGGA+AGG - chr5_1:54745381-54745400 None:intergenic 35.0%
CTTTGTTGGAATTCATTCAC+AGG + chr5_1:54745711-54745730 Msa0737500:intron 35.0%
ACAGAAAACAAGCATCATAG+AGG - chr5_1:54745938-54745957 None:intergenic 35.0%
AGATATGGCAATTGGGATAA+TGG + chr5_1:54744992-54745011 Msa0737500:exon 35.0%
GTACATCTTGTGAAAGCAAA+AGG + chr5_1:54745017-54745036 Msa0737500:CDS 35.0%
TAGAGGCAGAAATCATGTAT+GGG + chr5_1:54745228-54745247 Msa0737500:CDS 35.0%
! TTTTCTGCTGATGACTTCAT+TGG + chr5_1:54745334-54745353 Msa0737500:CDS 35.0%
! GTTAGGAGTATATATGTGGA+AGG - chr5_1:54745381-54745400 None:intergenic 35.0%
CTTTGTTGGAATTCATTCAC+AGG + chr5_1:54745711-54745730 Msa0737500:intron 35.0%
ACAGAAAACAAGCATCATAG+AGG - chr5_1:54745938-54745957 None:intergenic 35.0%
! AATACCAAGGTCAAGAGCAT+AGG + chr5_1:54745188-54745207 Msa0737500:CDS 40.0%
GTAGAGGCAGAAATCATGTA+TGG + chr5_1:54745227-54745246 Msa0737500:CDS 40.0%
! TTGGCTCAAGGTGTTCAAAA+TGG + chr5_1:54745494-54745513 Msa0737500:CDS 40.0%
ACAACTCTATACTTGAGAGG+TGG - chr5_1:54745533-54745552 None:intergenic 40.0%
CGAACAACTCTATACTTGAG+AGG - chr5_1:54745536-54745555 None:intergenic 40.0%
CAATCTCTCCACGATAAGAT+TGG - chr5_1:54745565-54745584 None:intergenic 40.0%
TGTTGGAATTCATTCACAGG+TGG + chr5_1:54745714-54745733 Msa0737500:intron 40.0%
AATTCATTCACAGGTGGAAG+AGG + chr5_1:54745720-54745739 Msa0737500:intron 40.0%
TTGAAGAAGACATAGGAGGA+TGG + chr5_1:54745749-54745768 Msa0737500:CDS 40.0%
! AATACCAAGGTCAAGAGCAT+AGG + chr5_1:54745188-54745207 Msa0737500:CDS 40.0%
GTAGAGGCAGAAATCATGTA+TGG + chr5_1:54745227-54745246 Msa0737500:CDS 40.0%
! TTGGCTCAAGGTGTTCAAAA+TGG + chr5_1:54745494-54745513 Msa0737500:CDS 40.0%
ACAACTCTATACTTGAGAGG+TGG - chr5_1:54745533-54745552 None:intergenic 40.0%
CGAACAACTCTATACTTGAG+AGG - chr5_1:54745536-54745555 None:intergenic 40.0%
CAATCTCTCCACGATAAGAT+TGG - chr5_1:54745565-54745584 None:intergenic 40.0%
TGTTGGAATTCATTCACAGG+TGG + chr5_1:54745714-54745733 Msa0737500:intron 40.0%
AATTCATTCACAGGTGGAAG+AGG + chr5_1:54745720-54745739 Msa0737500:intron 40.0%
TTGAAGAAGACATAGGAGGA+TGG + chr5_1:54745749-54745768 Msa0737500:CDS 40.0%
!! TGGAAGGCATTAGTCTTACG+TGG - chr5_1:54745365-54745384 None:intergenic 45.0%
!! TGGAAGGCATTAGTCTTACG+TGG - chr5_1:54745365-54745384 None:intergenic 45.0%
!!! TTTTTACTATATTAATATAT+AGG + chr5_1:54745136-54745155 Msa0737500:intron 5.0%
!!! TTTTTACTATATTAATATAT+AGG + chr5_1:54745136-54745155 Msa0737500:intron 5.0%
CGTGCAGACCAATCTTATCG+TGG + chr5_1:54745554-54745573 Msa0737500:CDS 50.0%
CGTGCAGACCAATCTTATCG+TGG + chr5_1:54745554-54745573 Msa0737500:CDS 50.0%
GGCTCCTATGCTCTTGACCT+TGG - chr5_1:54745195-54745214 None:intergenic 55.0%
! TCAAGAGCATAGGAGCCGTG+TGG + chr5_1:54745198-54745217 Msa0737500:CDS 55.0%
CATAGGAGCCGTGTGGCTAA+GGG + chr5_1:54745205-54745224 Msa0737500:CDS 55.0%
TGCCTCTACCCTTAGCCACA+CGG - chr5_1:54745216-54745235 None:intergenic 55.0%
GGCTCCTATGCTCTTGACCT+TGG - chr5_1:54745195-54745214 None:intergenic 55.0%
! TCAAGAGCATAGGAGCCGTG+TGG + chr5_1:54745198-54745217 Msa0737500:CDS 55.0%
CATAGGAGCCGTGTGGCTAA+GGG + chr5_1:54745205-54745224 Msa0737500:CDS 55.0%
TGCCTCTACCCTTAGCCACA+CGG - chr5_1:54745216-54745235 None:intergenic 55.0%
GCATAGGAGCCGTGTGGCTA+AGG + chr5_1:54745204-54745223 Msa0737500:CDS 60.0%
AGCCGTGTGGCTAAGGGTAG+AGG + chr5_1:54745211-54745230 Msa0737500:CDS 60.0%
GCATAGGAGCCGTGTGGCTA+AGG + chr5_1:54745204-54745223 Msa0737500:CDS 60.0%
AGCCGTGTGGCTAAGGGTAG+AGG + chr5_1:54745211-54745230 Msa0737500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 54744910 54746001 54744910 ID=Msa0737500;Name=Msa0737500
chr5_1 mRNA 54744910 54746001 54744910 ID=Msa0737500-mRNA-1;Parent=Msa0737500;Name=Msa0737500-mRNA-1;_AED=0.05;_eAED=0.05;_QI=86|1|1|1|1|1|4|203|158
chr5_1 exon 54744910 54745062 54744910 ID=Msa0737500-mRNA-1:exon:15353;Parent=Msa0737500-mRNA-1
chr5_1 exon 54745158 54745365 54745158 ID=Msa0737500-mRNA-1:exon:15354;Parent=Msa0737500-mRNA-1
chr5_1 exon 54745475 54745610 54745475 ID=Msa0737500-mRNA-1:exon:15355;Parent=Msa0737500-mRNA-1
chr5_1 exon 54745733 54746001 54745733 ID=Msa0737500-mRNA-1:exon:15356;Parent=Msa0737500-mRNA-1
chr5_1 five_prime_UTR 54744910 54744995 54744910 ID=Msa0737500-mRNA-1:five_prime_utr;Parent=Msa0737500-mRNA-1
chr5_1 CDS 54744996 54745062 54744996 ID=Msa0737500-mRNA-1:cds;Parent=Msa0737500-mRNA-1
chr5_1 CDS 54745158 54745365 54745158 ID=Msa0737500-mRNA-1:cds;Parent=Msa0737500-mRNA-1
chr5_1 CDS 54745475 54745610 54745475 ID=Msa0737500-mRNA-1:cds;Parent=Msa0737500-mRNA-1
chr5_1 CDS 54745733 54745798 54745733 ID=Msa0737500-mRNA-1:cds;Parent=Msa0737500-mRNA-1
chr5_1 three_prime_UTR 54745799 54746001 54745799 ID=Msa0737500-mRNA-1:three_prime_utr;Parent=Msa0737500-mRNA-1
Gene Sequence

>Msa0737500

ATGGCAATTGGGATAATGGAAGTACATCTTGTGAAAGCAAAAGGCCTCAATAACACTTGTTTCTTTGGTGGAATGGAAGATCCCTATGTTTTGATACAATACCAAGGTCAAGAGCATAGGAGCCGTGTGGCTAAGGGTAGAGGCAGAAATCATGTATGGGATGAGATATTTATGTTCAAAGTAGAAAACATTGGATCAAATGATAAAAACAAAGTCATTCTCAAAATAATGGACAAAGATAGTTTTTCTGCTGATGACTTCATTGGACAAGCCACAATCTATTGTAAAGATTTATTGGCTCAAGGTGTTCAAAATGGAGTTGCTAAGCTACCACCTCTCAAGTATAGAGTTGTTCGTGCAGACCAATCTTATCGTGGAGAGATTGAAGTTGCTATTACTTTTACTCCAAAGGTGGAAGAGGAATTAATTGAAGAAGACATAGGAGGATGGAAAGAAAGTAGTTACTATATATCTTAA

Protein sequence

>Msa0737500

MAIGIMEVHLVKAKGLNNTCFFGGMEDPYVLIQYQGQEHRSRVAKGRGRNHVWDEIFMFKVENIGSNDKNKVILKIMDKDSFSADDFIGQATIYCKDLLAQGVQNGVAKLPPLKYRVVRADQSYRGEIEVAITFTPKVEEELIEEDIGGWKESSYYIS*