AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0784630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 128 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATGATTATATGATAAT+TGG 0.136634 5_2:+69257674 Msa0784630:three_prime_UTR
TAGCTACAGCACAATGATTT+TGG 0.138385 5_2:+69257358 Msa0784630:three_prime_UTR
GCTAGGTTGCTTGGGGAAAA+TGG 0.147100 5_2:+69256633 Msa0784630:three_prime_UTR
GAAGCTTCAATAGGTTATTT+TGG 0.219887 5_2:-69256138 None:intergenic
GATAACTAAGAAAGAATTTC+GGG 0.237583 5_2:-69256416 None:intergenic
TGAGCTTCTTAGACAGGTTT+TGG 0.251317 5_2:+69256890 Msa0784630:three_prime_UTR
AGTTACAACTCTCTGATTAA+AGG 0.289233 5_2:+69257464 Msa0784630:three_prime_UTR
TAGAGGTTATGCAGAAAATT+TGG 0.294463 5_2:+69257334 Msa0784630:three_prime_UTR
TGCTCAAAAGTCTGCATATT+GGG 0.296680 5_2:-69257767 None:intergenic
AGGGTTATGCTAGGTTGCTT+GGG 0.297743 5_2:+69256625 Msa0784630:three_prime_UTR
AACTGTTGGGTGTTTGATTA+AGG 0.300973 5_2:+69256833 Msa0784630:three_prime_UTR
TTTAATGATCTTAAGGATAG+AGG 0.308650 5_2:+69257107 Msa0784630:three_prime_UTR
AGATAACTAAGAAAGAATTT+CGG 0.311149 5_2:-69256417 None:intergenic
GAGGGTTATGCTAGGTTGCT+TGG 0.315506 5_2:+69256624 Msa0784630:three_prime_UTR
ACTGTTTAAAACGAGAAAAT+AGG 0.320597 5_2:+69257798 Msa0784630:three_prime_UTR
ACGTGGTTTAGTTCGGGTTA+TGG 0.322164 5_2:-69256346 None:intergenic
CCTTAACTAGCTCGCCTTCC+TGG 0.330031 5_2:-69257493 None:intergenic
TATTGGCTTGTTTGAAAGTT+GGG 0.337316 5_2:+69256739 Msa0784630:three_prime_UTR
TTTCCCTTCGTTTCCTTCAC+TGG 0.337635 5_2:+69256463 Msa0784630:three_prime_UTR
TGTTCCGAAGAATGATTCTT+TGG 0.340309 5_2:+69256602 Msa0784630:three_prime_UTR
TTGGGAGGACTGATTTGGTT+TGG 0.346755 5_2:+69256757 Msa0784630:three_prime_UTR
TTTCCCAACGTGGTTTAGTT+CGG 0.347682 5_2:-69256353 None:intergenic
TTCCCAACGTGGTTTAGTTC+GGG 0.357239 5_2:-69256352 None:intergenic
TTGATCTTTAGGATCTTGTT+TGG 0.358934 5_2:-69256292 None:intergenic
CCAAACACAAAGGAGGAAAA+AGG 0.359317 5_2:-69257925 None:intergenic
ACTTATCAAGAGATTATTCA+TGG 0.363261 5_2:+69257044 Msa0784630:three_prime_UTR
TTGTTTCTGTGGTGAGGTTT+CGG 0.369531 5_2:-69256266 None:intergenic
CATCTCAAACCACAACTTCC+TGG 0.372583 5_2:-69257201 None:intergenic
ACGTATAATGTAATGATATA+TGG 0.376012 5_2:+69257254 Msa0784630:three_prime_UTR
GAAAGTTGGGAGGACTGATT+TGG 0.377688 5_2:+69256752 Msa0784630:three_prime_UTR
TATGAGCATATGATTGAATC+TGG 0.380517 5_2:+69256786 Msa0784630:three_prime_UTR
TTTGATGCTCTTGTTGATGC+TGG 0.396467 5_2:+69256537 Msa0784630:three_prime_UTR
ATAAAATGTCTTTGCAAAGT+TGG 0.397144 5_2:+69257584 Msa0784630:three_prime_UTR
ATATGATTGAATCTGGGGTT+GGG 0.403623 5_2:+69256793 Msa0784630:three_prime_UTR
CACATGATTATATGATAATT+GGG 0.407028 5_2:+69257675 Msa0784630:three_prime_UTR
TCATTCTTCGGAACAAAATC+AGG 0.417363 5_2:-69256594 None:intergenic
TGGTTTGAGATGATTCAAAA+AGG 0.417493 5_2:+69257212 Msa0784630:three_prime_UTR
GTGCTCAAAAGTCTGCATAT+TGG 0.418414 5_2:-69257768 None:intergenic
AATGATATATGGTTATTGTA+AGG 0.426559 5_2:+69257265 Msa0784630:three_prime_UTR
CGTGGCACCGAAGCTTCAAT+AGG 0.432572 5_2:-69256147 None:intergenic
TTCACATTGTGGTTTCAAAC+CGG 0.433912 5_2:+69256491 Msa0784630:three_prime_UTR
CCAGGAAGGCGAGCTAGTTA+AGG 0.434932 5_2:+69257493 Msa0784630:three_prime_UTR
AGATATGCATGATAGGCATT+TGG 0.437930 5_2:+69257637 Msa0784630:three_prime_UTR
AAAATAACCTATTGAAGCTT+CGG 0.439177 5_2:+69256140 Msa0784630:three_prime_UTR
TTTGTTAGTTTGAAAGAGGT+TGG 0.442556 5_2:+69256681 Msa0784630:three_prime_UTR
TAATCATGTGTACTAGCAAT+TGG 0.446374 5_2:-69257662 None:intergenic
TCTGAGTGACATTGTCATCA+TGG 0.446532 5_2:-69256223 None:intergenic
TGCGTATGAGCTTCTTAGAC+AGG 0.446776 5_2:+69256884 Msa0784630:three_prime_UTR
ATTCAACGAACTTCTTATGC+AGG 0.447523 5_2:+69257526 Msa0784630:three_prime_UTR
ACATTATACGTGTACTCATT+CGG 0.449189 5_2:-69257242 None:intergenic
AACCCGAACTAAACCACGTT+GGG 0.449791 5_2:+69256350 Msa0784630:three_prime_UTR
CTAGGTTGCTTGGGGAAAAT+GGG 0.452180 5_2:+69256634 Msa0784630:three_prime_UTR
CACTGGCTCACTTCACATTG+TGG 0.454789 5_2:+69256480 Msa0784630:three_prime_UTR
TTATTGGCTTGTTTGAAAGT+TGG 0.455824 5_2:+69256738 Msa0784630:three_prime_UTR
TTCAAAGGTTGTTGTCGGAT+TGG 0.457536 5_2:-69256179 None:intergenic
TATGGTGCAGATTTCGTTGA+GGG 0.462402 5_2:-69256328 None:intergenic
CCCTCCAAAGAATCATTCTT+CGG 0.464196 5_2:-69256606 None:intergenic
AATATTGATGTTGAAACTGT+TGG 0.465621 5_2:+69256819 Msa0784630:three_prime_UTR
GTTGTATACATAACCCTGTC+TGG 0.470369 5_2:-69257137 None:intergenic
TGTTGAAAGATATGCATGAT+AGG 0.470710 5_2:+69257630 Msa0784630:three_prime_UTR
ACAGTTTCAAAGGTTGTTGT+CGG 0.475154 5_2:-69256184 None:intergenic
GAAGAGAGTGCGCAGGAGCT+TGG 0.478717 5_2:-69254516 None:intergenic
TAGATTTAATGTTTAGAGAA+GGG 0.480778 5_2:+69257843 Msa0784630:three_prime_UTR
GGTTTGAGAAATTGAAAGAT+GGG 0.486122 5_2:-69256395 None:intergenic
TGGGGTTGCTTGCTGAGGCC+AGG 0.487470 5_2:+69257183 Msa0784630:three_prime_UTR
ACAACTTGAAGGTTTACAAA+GGG 0.488087 5_2:-69258067 None:intergenic
GCATTATATGAAGATTGATC+CGG 0.493959 5_2:-69256510 None:intergenic
GCGTACATATAACAACTTGA+AGG 0.495793 5_2:-69258078 None:intergenic
CCGAAGAATGATTCTTTGGA+GGG 0.496823 5_2:+69256606 Msa0784630:three_prime_UTR
CTGAGTGACATTGTCATCAT+GGG 0.497507 5_2:-69256222 None:intergenic
TGGGGAAAATGGGATGGTTG+AGG 0.498283 5_2:+69256644 Msa0784630:three_prime_UTR
TCTGATTAAAGGCCTTTGCC+AGG 0.500511 5_2:+69257475 Msa0784630:three_prime_UTR
CATATGATTGAATCTGGGGT+TGG 0.502079 5_2:+69256792 Msa0784630:three_prime_UTR
GGGTTTGAGAAATTGAAAGA+TGG 0.502373 5_2:-69256396 None:intergenic
ATATTGATGTTGAAACTGTT+GGG 0.505327 5_2:+69256820 Msa0784630:three_prime_UTR
TTAGATTTAATGTTTAGAGA+AGG 0.511904 5_2:+69257842 Msa0784630:three_prime_UTR
TATGATTGAATCTGGGGTTG+GGG 0.514223 5_2:+69256794 Msa0784630:three_prime_UTR
TTAATGATCTTAAGGATAGA+GGG 0.516349 5_2:+69257108 Msa0784630:three_prime_UTR
AAGATGGGTATTGTGAAAGA+AGG 0.516570 5_2:-69256380 None:intergenic
TTGTTTGGTTGTTGTTTCTG+TGG 0.518610 5_2:-69256277 None:intergenic
TGTGTTTGTTAGTTTGAAAG+AGG 0.519159 5_2:+69256677 Msa0784630:three_prime_UTR
GCGAAAGAGGAGAGAAATTG+AGG 0.524835 5_2:-69254573 None:intergenic
TCCGAAGAATGATTCTTTGG+AGG 0.528081 5_2:+69256605 Msa0784630:three_prime_UTR
ATGTATACAACGGTTATCAA+AGG 0.530143 5_2:+69257149 Msa0784630:three_prime_UTR
TGGTTGTTGTTTCTGTGGTG+AGG 0.532400 5_2:-69256272 None:intergenic
AAGGAAACGAAGGGAAAGGA+AGG 0.535168 5_2:-69256457 None:intergenic
GCGCAGGAGCTTGGCAATGG+CGG 0.537744 5_2:-69254507 None:intergenic
TGATGTGAGAGCGCATCGAA+TGG 0.539610 5_2:-69254538 None:intergenic
TGTGAATGAAACAGTTTCAA+AGG 0.543255 5_2:-69256194 None:intergenic
TTTCATGAAATGTCGCGAAA+GGG 0.544857 5_2:+69257422 Msa0784630:three_prime_UTR
AATCATCATTTAATAGTACA+TGG 0.545178 5_2:-69257997 None:intergenic
AGTGCGCAGGAGCTTGGCAA+TGG 0.547796 5_2:-69254510 None:intergenic
TTCAACGAACTTCTTATGCA+GGG 0.548937 5_2:+69257527 Msa0784630:three_prime_UTR
AAGAAAATTACCTTGAATTG+CGG 0.551939 5_2:-69254610 None:intergenic
AGATGGGTATTGTGAAAGAA+GGG 0.552728 5_2:-69256379 None:intergenic
ATTCTTTGGAGGGTTATGCT+AGG 0.552937 5_2:+69256616 Msa0784630:three_prime_UTR
ATTAAAGGCCTTTGCCAGGA+AGG 0.553517 5_2:+69257479 Msa0784630:three_prime_UTR
GGTTGCTAAAGATGCTGAGT+TGG 0.557716 5_2:+69257735 Msa0784630:three_prime_UTR
GAAGAGGAAGAAGAATGATG+AGG 0.559419 5_2:+69257073 Msa0784630:three_prime_UTR
TTGCTGAGGCCAGGAAGTTG+TGG 0.561607 5_2:+69257192 Msa0784630:three_prime_UTR
GTTTCATGAAATGTCGCGAA+AGG 0.562910 5_2:+69257421 Msa0784630:three_prime_UTR
CTAGCTCGCCTTCCTGGCAA+AGG 0.572227 5_2:-69257487 None:intergenic
GGGTTATGCTAGGTTGCTTG+GGG 0.575307 5_2:+69256626 Msa0784630:three_prime_UTR
TATGATGATATGTGTGGTAG+AGG 0.577409 5_2:+69257317 Msa0784630:three_prime_UTR
TGATATGGGGTTGCTTGCTG+AGG 0.578172 5_2:+69257178 Msa0784630:three_prime_UTR
TGCAAAGTTGGAGACACTGA+GGG 0.588567 5_2:+69257596 Msa0784630:three_prime_UTR
AGTGAAGGAAACGAAGGGAA+AGG 0.592056 5_2:-69256461 None:intergenic
AACAACTTGAAGGTTTACAA+AGG 0.592729 5_2:-69258068 None:intergenic
TTATGGTGCAGATTTCGTTG+AGG 0.598996 5_2:-69256329 None:intergenic
ATGAGCATATGATTGAATCT+GGG 0.609681 5_2:+69256787 Msa0784630:three_prime_UTR
TGGCTTGTTTGAAAGTTGGG+AGG 0.614610 5_2:+69256742 Msa0784630:three_prime_UTR
GGAGTAAAGGAAGAAACAGA+TGG 0.618721 5_2:-69257557 None:intergenic
TCTTGATAAGTATAGATACT+CGG 0.625160 5_2:-69257032 None:intergenic
GTTGCTTGGGGAAAATGGGA+TGG 0.629510 5_2:+69256638 Msa0784630:three_prime_UTR
AATGTGAAGTGAGCCAGTGA+AGG 0.629610 5_2:-69256476 None:intergenic
TCGAATGGAAGAGAGTGCGC+AGG 0.633972 5_2:-69254523 None:intergenic
TGAGCCAGTGAAGGAAACGA+AGG 0.636495 5_2:-69256467 None:intergenic
TCGGATTGGAACAGCGTACG+TGG 0.642357 5_2:-69256165 None:intergenic
TAACCCGAACTAAACCACGT+TGG 0.647364 5_2:+69256349 Msa0784630:three_prime_UTR
ACATTAAAACTCAACACAGA+CGG 0.654839 5_2:-69256711 None:intergenic
GAGCCAGTGAAGGAAACGAA+GGG 0.656330 5_2:-69256466 None:intergenic
GCGAGCTCCTCCAAACACAA+AGG 0.670155 5_2:-69257935 None:intergenic
TGAGCATATGATTGAATCTG+GGG 0.670828 5_2:+69256788 Msa0784630:three_prime_UTR
GGTGAGGTTTCGGAAATACG+AGG 0.677848 5_2:-69256256 None:intergenic
AGCTCCTCCAAACACAAAGG+AGG 0.685602 5_2:-69257932 None:intergenic
AGACAGGGTTATGTATACAA+CGG 0.685868 5_2:+69257139 Msa0784630:three_prime_UTR
TTGCAAAGTTGGAGACACTG+AGG 0.690667 5_2:+69257595 Msa0784630:three_prime_UTR
AAGCTCTATGATGATATGTG+TGG 0.757559 5_2:+69257311 Msa0784630:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTCAAGTAATTAAAAAA+TGG - chr5_2:69255305-69255324 None:intergenic 10.0%
!!! GTTTTAAATTACTTTTTTTT+AGG - chr5_2:69255355-69255374 None:intergenic 10.0%
!! CTTAATTAAAATAATTAAGT+TGG - chr5_2:69255385-69255404 None:intergenic 10.0%
!!! TTTTCAAATAAAATAAGAAT+TGG - chr5_2:69256076-69256095 None:intergenic 10.0%
!!! ATGATGATTTTTTTTTTTTT+TGG + chr5_2:69258011-69258030 Msa0784630:three_prime_UTR 10.0%
!!! TGATGATTTTTTTTTTTTTT+GGG + chr5_2:69258012-69258031 Msa0784630:three_prime_UTR 10.0%
!!! TAAATTTTGTAAGATTGTTT+GGG - chr5_2:69254983-69255002 None:intergenic 15.0%
!!! ATAAATTTTGTAAGATTGTT+TGG - chr5_2:69254984-69255003 None:intergenic 15.0%
!!! GATATTGATTATATTTTTTG+AGG + chr5_2:69255215-69255234 Msa0784630:intron 15.0%
!! GAAAATCTTTCAAAAAAAAA+TGG - chr5_2:69255279-69255298 None:intergenic 15.0%
!!! GATGATTTTTTTTTTTTTTG+GGG + chr5_2:69258013-69258032 Msa0784630:three_prime_UTR 15.0%
!! ATTACAAGTTATAACTTACA+GGG - chr5_2:69254874-69254893 None:intergenic 20.0%
!! AATTACAAGTTATAACTTAC+AGG - chr5_2:69254875-69254894 None:intergenic 20.0%
!! ATCTTTCAAAAAAAAATGGA+TGG - chr5_2:69255275-69255294 None:intergenic 20.0%
!! TCAAGTAATTAAAAAATGGA+TGG - chr5_2:69255301-69255320 None:intergenic 20.0%
!! AGATAACTAAGAAAGAATTT+CGG - chr5_2:69256420-69256439 None:intergenic 20.0%
!!! TAGAGTTTTTAATGATCTTA+AGG + chr5_2:69257100-69257119 Msa0784630:three_prime_UTR 20.0%
!! ACGTATAATGTAATGATATA+TGG + chr5_2:69257254-69257273 Msa0784630:three_prime_UTR 20.0%
!! AATGATATATGGTTATTGTA+AGG + chr5_2:69257265-69257284 Msa0784630:three_prime_UTR 20.0%
!! ACACATGATTATATGATAAT+TGG + chr5_2:69257674-69257693 Msa0784630:three_prime_UTR 20.0%
!! CACATGATTATATGATAATT+GGG + chr5_2:69257675-69257694 Msa0784630:three_prime_UTR 20.0%
!! ATAATTGGGTTAAGTAAAAA+AGG + chr5_2:69257689-69257708 Msa0784630:three_prime_UTR 20.0%
!! TTAGATTTAATGTTTAGAGA+AGG + chr5_2:69257842-69257861 Msa0784630:three_prime_UTR 20.0%
!! TAGATTTAATGTTTAGAGAA+GGG + chr5_2:69257843-69257862 Msa0784630:three_prime_UTR 20.0%
!! AATCATCATTTAATAGTACA+TGG - chr5_2:69258000-69258019 None:intergenic 20.0%
! AAGAAAATTACCTTGAATTG+CGG - chr5_2:69254613-69254632 None:intergenic 25.0%
!! TCATCTTTTCAATTTTCATC+TGG + chr5_2:69254644-69254663 Msa0784630:intron 25.0%
! AAAAAATGCTCAAAAGAATG+TGG - chr5_2:69254745-69254764 None:intergenic 25.0%
! AAAAAATAAGCCAATCCAAA+CGG - chr5_2:69255054-69255073 None:intergenic 25.0%
!! TTTTCTTAAGACTCGTATTA+AGG + chr5_2:69255422-69255441 Msa0784630:intron 25.0%
! CATGTTTCAAAGTTAGAATT+GGG - chr5_2:69255448-69255467 None:intergenic 25.0%
! ACATGTTTCAAAGTTAGAAT+TGG - chr5_2:69255449-69255468 None:intergenic 25.0%
!!! AAATCTTACTTTTGTTTGAG+AGG - chr5_2:69255576-69255595 None:intergenic 25.0%
!! TACAAGTTTTCTTCTACTAA+GGG - chr5_2:69255993-69256012 None:intergenic 25.0%
!! ATACAAGTTTTCTTCTACTA+AGG - chr5_2:69255994-69256013 None:intergenic 25.0%
! AAAATAACCTATTGAAGCTT+CGG + chr5_2:69256140-69256159 Msa0784630:three_prime_UTR 25.0%
!!! TGAGTAAAGTTTTGATCTTT+AGG - chr5_2:69256306-69256325 None:intergenic 25.0%
! GATAACTAAGAAAGAATTTC+GGG - chr5_2:69256419-69256438 None:intergenic 25.0%
!!! GAGTTTTAATGTGTGTTTAT+TGG + chr5_2:69256722-69256741 Msa0784630:three_prime_UTR 25.0%
! AATATTGATGTTGAAACTGT+TGG + chr5_2:69256819-69256838 Msa0784630:three_prime_UTR 25.0%
! ATATTGATGTTGAAACTGTT+GGG + chr5_2:69256820-69256839 Msa0784630:three_prime_UTR 25.0%
! TCTTGATAAGTATAGATACT+CGG - chr5_2:69257035-69257054 None:intergenic 25.0%
! ACTTATCAAGAGATTATTCA+TGG + chr5_2:69257044-69257063 Msa0784630:three_prime_UTR 25.0%
! TTTAATGATCTTAAGGATAG+AGG + chr5_2:69257107-69257126 Msa0784630:three_prime_UTR 25.0%
! TTAATGATCTTAAGGATAGA+GGG + chr5_2:69257108-69257127 Msa0784630:three_prime_UTR 25.0%
!! TATCAAAGGTTTTTGTGATA+TGG + chr5_2:69257163-69257182 Msa0784630:three_prime_UTR 25.0%
!! ATCAAAGGTTTTTGTGATAT+GGG + chr5_2:69257164-69257183 Msa0784630:three_prime_UTR 25.0%
! ATAAAATGTCTTTGCAAAGT+TGG + chr5_2:69257584-69257603 Msa0784630:three_prime_UTR 25.0%
!! ACTGTTTAAAACGAGAAAAT+AGG + chr5_2:69257798-69257817 Msa0784630:three_prime_UTR 25.0%
!! AAAAAGCAGAATTTGTTCTT+TGG + chr5_2:69257877-69257896 Msa0784630:three_prime_UTR 25.0%
!! GAATGAAATCAGACTGATTT+TGG + chr5_2:69254843-69254862 Msa0784630:intron 30.0%
TTAATCCAAACAGACACTTA+GGG + chr5_2:69255027-69255046 Msa0784630:intron 30.0%
!! TTTTGTCACTTGATGTTCTA+TGG - chr5_2:69255480-69255499 None:intergenic 30.0%
TTTGTTTGAGAGGTAGAAAA+TGG - chr5_2:69255566-69255585 None:intergenic 30.0%
ATAAGCTGTTTATCCAAACA+GGG - chr5_2:69255685-69255704 None:intergenic 30.0%
TATAAGCTGTTTATCCAAAC+AGG - chr5_2:69255686-69255705 None:intergenic 30.0%
TTACCAAGATAAGCTATAAG+CGG - chr5_2:69255726-69255745 None:intergenic 30.0%
AGAAGAAAACTTGTATCACA+TGG + chr5_2:69255998-69256017 Msa0784630:intron 30.0%
! GAAGCTTCAATAGGTTATTT+TGG - chr5_2:69256141-69256160 None:intergenic 30.0%
TGTGAATGAAACAGTTTCAA+AGG - chr5_2:69256197-69256216 None:intergenic 30.0%
!! TTGATCTTTAGGATCTTGTT+TGG - chr5_2:69256295-69256314 None:intergenic 30.0%
GGTTTGAGAAATTGAAAGAT+GGG - chr5_2:69256398-69256417 None:intergenic 30.0%
GCATTATATGAAGATTGATC+CGG - chr5_2:69256513-69256532 None:intergenic 30.0%
! TGTGTTTGTTAGTTTGAAAG+AGG + chr5_2:69256677-69256696 Msa0784630:three_prime_UTR 30.0%
! TTTGTTAGTTTGAAAGAGGT+TGG + chr5_2:69256681-69256700 Msa0784630:three_prime_UTR 30.0%
ACATTAAAACTCAACACAGA+CGG - chr5_2:69256714-69256733 None:intergenic 30.0%
!! TTATTGGCTTGTTTGAAAGT+TGG + chr5_2:69256738-69256757 Msa0784630:three_prime_UTR 30.0%
!! TATTGGCTTGTTTGAAAGTT+GGG + chr5_2:69256739-69256758 Msa0784630:three_prime_UTR 30.0%
TATGAGCATATGATTGAATC+TGG + chr5_2:69256786-69256805 Msa0784630:three_prime_UTR 30.0%
ATGAGCATATGATTGAATCT+GGG + chr5_2:69256787-69256806 Msa0784630:three_prime_UTR 30.0%
!! TTATTCATGGACTTTTGAAG+AGG + chr5_2:69257057-69257076 Msa0784630:three_prime_UTR 30.0%
ATGTATACAACGGTTATCAA+AGG + chr5_2:69257149-69257168 Msa0784630:three_prime_UTR 30.0%
! TCAAAGGTTTTTGTGATATG+GGG + chr5_2:69257165-69257184 Msa0784630:three_prime_UTR 30.0%
! TGGTTTGAGATGATTCAAAA+AGG + chr5_2:69257212-69257231 Msa0784630:three_prime_UTR 30.0%
ACATTATACGTGTACTCATT+CGG - chr5_2:69257245-69257264 None:intergenic 30.0%
TAGAGGTTATGCAGAAAATT+TGG + chr5_2:69257334-69257353 Msa0784630:three_prime_UTR 30.0%
AGTTACAACTCTCTGATTAA+AGG + chr5_2:69257464-69257483 Msa0784630:three_prime_UTR 30.0%
! ACATTTTATGAGAGGAGTAA+AGG - chr5_2:69257573-69257592 None:intergenic 30.0%
! TTGCAAAGACATTTTATGAG+AGG - chr5_2:69257581-69257600 None:intergenic 30.0%
TGTTGAAAGATATGCATGAT+AGG + chr5_2:69257630-69257649 Msa0784630:three_prime_UTR 30.0%
TAATCATGTGTACTAGCAAT+TGG - chr5_2:69257665-69257684 None:intergenic 30.0%
! AAAAAAGGAGATTTTGCACA+AGG + chr5_2:69257704-69257723 Msa0784630:three_prime_UTR 30.0%
ACAACTTGAAGGTTTACAAA+GGG - chr5_2:69258070-69258089 None:intergenic 30.0%
AACAACTTGAAGGTTTACAA+AGG - chr5_2:69258071-69258090 None:intergenic 30.0%
! AAAAATGGATTCAGCGAAAG+AGG - chr5_2:69254589-69254608 None:intergenic 35.0%
GTGGCATATGTCATATGAAA+CGG - chr5_2:69254726-69254745 None:intergenic 35.0%
GTTAATCCAAACAGACACTT+AGG + chr5_2:69255026-69255045 Msa0784630:intron 35.0%
AAAATATGTCCTCGTGAGTT+TGG - chr5_2:69255143-69255162 None:intergenic 35.0%
AAGTGACAAAACTATGCGTT+GGG + chr5_2:69255489-69255508 Msa0784630:intron 35.0%
AGGGTCAAAATCAAAGAACA+GGG - chr5_2:69255666-69255685 None:intergenic 35.0%
!! TCTTTGATTTTGACCCTGTT+TGG + chr5_2:69255669-69255688 Msa0784630:intron 35.0%
CACAAGTAAGTCAATCCAAA+CGG + chr5_2:69255928-69255947 Msa0784630:intron 35.0%
ACAAGTAAGTCAATCCAAAC+GGG + chr5_2:69255929-69255948 Msa0784630:intron 35.0%
AGAACTCTCAAACAGCTTAT+GGG - chr5_2:69255955-69255974 None:intergenic 35.0%
AAGAACTCTCAAACAGCTTA+TGG - chr5_2:69255956-69255975 None:intergenic 35.0%
AAGCTGTTTGAGAGTTCTTA+TGG + chr5_2:69255957-69255976 Msa0784630:intron 35.0%
TGTTTGAGAGTTCTTATGGA+TGG + chr5_2:69255961-69255980 Msa0784630:intron 35.0%
TATCACATGGAAATAGAGCA+AGG + chr5_2:69256011-69256030 Msa0784630:intron 35.0%
ATCACATGGAAATAGAGCAA+GGG + chr5_2:69256012-69256031 Msa0784630:intron 35.0%
ACAGTTTCAAAGGTTGTTGT+CGG - chr5_2:69256187-69256206 None:intergenic 35.0%
!! TTGTTTGGTTGTTGTTTCTG+TGG - chr5_2:69256280-69256299 None:intergenic 35.0%
! AGATGGGTATTGTGAAAGAA+GGG - chr5_2:69256382-69256401 None:intergenic 35.0%
! AAGATGGGTATTGTGAAAGA+AGG - chr5_2:69256383-69256402 None:intergenic 35.0%
GGGTTTGAGAAATTGAAAGA+TGG - chr5_2:69256399-69256418 None:intergenic 35.0%
TTCACATTGTGGTTTCAAAC+CGG + chr5_2:69256491-69256510 Msa0784630:three_prime_UTR 35.0%
TCATTCTTCGGAACAAAATC+AGG - chr5_2:69256597-69256616 None:intergenic 35.0%
TGTTCCGAAGAATGATTCTT+TGG + chr5_2:69256602-69256621 Msa0784630:three_prime_UTR 35.0%
TGAGCATATGATTGAATCTG+GGG + chr5_2:69256788-69256807 Msa0784630:three_prime_UTR 35.0%
! ATATGATTGAATCTGGGGTT+GGG + chr5_2:69256793-69256812 Msa0784630:three_prime_UTR 35.0%
AACTGTTGGGTGTTTGATTA+AGG + chr5_2:69256833-69256852 Msa0784630:three_prime_UTR 35.0%
!! TTTGATTAAGGCGTTTTGTG+CGG + chr5_2:69256845-69256864 Msa0784630:three_prime_UTR 35.0%
! GTTTTTAACGCGCTTATAAC+TGG + chr5_2:69256939-69256958 Msa0784630:three_prime_UTR 35.0%
AGACAGGGTTATGTATACAA+CGG + chr5_2:69257139-69257158 Msa0784630:three_prime_UTR 35.0%
AAGCTCTATGATGATATGTG+TGG + chr5_2:69257311-69257330 Msa0784630:three_prime_UTR 35.0%
TATGATGATATGTGTGGTAG+AGG + chr5_2:69257317-69257336 Msa0784630:three_prime_UTR 35.0%
! TAGCTACAGCACAATGATTT+TGG + chr5_2:69257358-69257377 Msa0784630:three_prime_UTR 35.0%
! AGCTACAGCACAATGATTTT+GGG + chr5_2:69257359-69257378 Msa0784630:three_prime_UTR 35.0%
TTTCATGAAATGTCGCGAAA+GGG + chr5_2:69257422-69257441 Msa0784630:three_prime_UTR 35.0%
ATTCAACGAACTTCTTATGC+AGG + chr5_2:69257526-69257545 Msa0784630:three_prime_UTR 35.0%
TTCAACGAACTTCTTATGCA+GGG + chr5_2:69257527-69257546 Msa0784630:three_prime_UTR 35.0%
AGATATGCATGATAGGCATT+TGG + chr5_2:69257637-69257656 Msa0784630:three_prime_UTR 35.0%
! ATTTTGCACAAGGTATGGAA+TGG + chr5_2:69257714-69257733 Msa0784630:three_prime_UTR 35.0%
TGCTCAAAAGTCTGCATATT+GGG - chr5_2:69257770-69257789 None:intergenic 35.0%
!! CTCGTTTTAAACAGTCAATC+AGG - chr5_2:69257793-69257812 None:intergenic 35.0%
GCGTACATATAACAACTTGA+AGG - chr5_2:69258081-69258100 None:intergenic 35.0%
!! TCCATTTTTGCCGCAATTCA+AGG + chr5_2:69254600-69254619 Msa0784630:three_prime_UTR 40.0%
ACCTTGAATTGCGGCAAAAA+TGG - chr5_2:69254604-69254623 None:intergenic 40.0%
ATGGGCACAGAGAGAAAAAT+CGG - chr5_2:69254681-69254700 None:intergenic 40.0%
ATGAAGATACCAAACTCACG+AGG + chr5_2:69255131-69255150 Msa0784630:intron 40.0%
CAAGTGACAAAACTATGCGT+TGG + chr5_2:69255488-69255507 Msa0784630:intron 40.0%
GTTTGAGAGGTAGAAAATGG+CGG - chr5_2:69255563-69255582 None:intergenic 40.0%
CAGGGTCAAAATCAAAGAAC+AGG - chr5_2:69255667-69255686 None:intergenic 40.0%
TTCAAAGGTTGTTGTCGGAT+TGG - chr5_2:69256182-69256201 None:intergenic 40.0%
CTGAGTGACATTGTCATCAT+GGG - chr5_2:69256225-69256244 None:intergenic 40.0%
TCTGAGTGACATTGTCATCA+TGG - chr5_2:69256226-69256245 None:intergenic 40.0%
!! TTGTTTCTGTGGTGAGGTTT+CGG - chr5_2:69256269-69256288 None:intergenic 40.0%
TATGGTGCAGATTTCGTTGA+GGG - chr5_2:69256331-69256350 None:intergenic 40.0%
TTATGGTGCAGATTTCGTTG+AGG - chr5_2:69256332-69256351 None:intergenic 40.0%
TTTCCCAACGTGGTTTAGTT+CGG - chr5_2:69256356-69256375 None:intergenic 40.0%
TTTGATGCTCTTGTTGATGC+TGG + chr5_2:69256537-69256556 Msa0784630:three_prime_UTR 40.0%
TCCGAAGAATGATTCTTTGG+AGG + chr5_2:69256605-69256624 Msa0784630:three_prime_UTR 40.0%
CCCTCCAAAGAATCATTCTT+CGG - chr5_2:69256609-69256628 None:intergenic 40.0%
CCGAAGAATGATTCTTTGGA+GGG + chr5_2:69256606-69256625 Msa0784630:three_prime_UTR 40.0%
! ATTCTTTGGAGGGTTATGCT+AGG + chr5_2:69256616-69256635 Msa0784630:three_prime_UTR 40.0%
! CATATGATTGAATCTGGGGT+TGG + chr5_2:69256792-69256811 Msa0784630:three_prime_UTR 40.0%
! TATGATTGAATCTGGGGTTG+GGG + chr5_2:69256794-69256813 Msa0784630:three_prime_UTR 40.0%
! TGAGCTTCTTAGACAGGTTT+TGG + chr5_2:69256890-69256909 Msa0784630:three_prime_UTR 40.0%
!!! TCTTAGACAGGTTTTGGAGA+AGG + chr5_2:69256896-69256915 Msa0784630:three_prime_UTR 40.0%
!!! CTTAGACAGGTTTTGGAGAA+GGG + chr5_2:69256897-69256916 Msa0784630:three_prime_UTR 40.0%
GAAGAGGAAGAAGAATGATG+AGG + chr5_2:69257073-69257092 Msa0784630:three_prime_UTR 40.0%
!!! ATAGAGGGTATTTTCCAGAC+AGG + chr5_2:69257123-69257142 Msa0784630:three_prime_UTR 40.0%
!!! TAGAGGGTATTTTCCAGACA+GGG + chr5_2:69257124-69257143 Msa0784630:three_prime_UTR 40.0%
GTTGTATACATAACCCTGTC+TGG - chr5_2:69257140-69257159 None:intergenic 40.0%
! TAAGGTTCGTGATTTTGCAG+AGG + chr5_2:69257283-69257302 Msa0784630:three_prime_UTR 40.0%
!! ATTTTGGGTCTGTGTTTGCA+TGG + chr5_2:69257374-69257393 Msa0784630:three_prime_UTR 40.0%
GTTTCATGAAATGTCGCGAA+AGG + chr5_2:69257421-69257440 Msa0784630:three_prime_UTR 40.0%
GGAGTAAAGGAAGAAACAGA+TGG - chr5_2:69257560-69257579 None:intergenic 40.0%
! AGGAGATTTTGCACAAGGTA+TGG + chr5_2:69257709-69257728 Msa0784630:three_prime_UTR 40.0%
GTGCTCAAAAGTCTGCATAT+TGG - chr5_2:69257771-69257790 None:intergenic 40.0%
CCAAACACAAAGGAGGAAAA+AGG - chr5_2:69257928-69257947 None:intergenic 40.0%
! CCTTTTTCCTCCTTTGTGTT+TGG + chr5_2:69257925-69257944 Msa0784630:three_prime_UTR 40.0%
! TTTTCCTCCTTTGTGTTTGG+AGG + chr5_2:69257928-69257947 Msa0784630:three_prime_UTR 40.0%
GCGAAAGAGGAGAGAAATTG+AGG - chr5_2:69254576-69254595 None:intergenic 45.0%
! AATGCCAACCATGGAAGCAT+GGG - chr5_2:69254699-69254718 None:intergenic 45.0%
TAATGCCAACCATGGAAGCA+TGG - chr5_2:69254700-69254719 None:intergenic 45.0%
AACGGAAGTAATGCCAACCA+TGG - chr5_2:69254708-69254727 None:intergenic 45.0%
TAGAAAATGGCGGTCATGCA+GGG - chr5_2:69255553-69255572 None:intergenic 45.0%
CAGGGTGATGAGAATTAAGC+AGG - chr5_2:69255648-69255667 None:intergenic 45.0%
CTATAAGCGGCTGTGCTATA+AGG - chr5_2:69255713-69255732 None:intergenic 45.0%
CAGCCGCTTATAGCTTATCT+TGG + chr5_2:69255720-69255739 Msa0784630:intron 45.0%
TGGAAATAGAGCAAGGGCAA+TGG + chr5_2:69256018-69256037 Msa0784630:intron 45.0%
!! TGGTTGTTGTTTCTGTGGTG+AGG - chr5_2:69256275-69256294 None:intergenic 45.0%
! ACGTGGTTTAGTTCGGGTTA+TGG - chr5_2:69256349-69256368 None:intergenic 45.0%
TAACCCGAACTAAACCACGT+TGG + chr5_2:69256349-69256368 Msa0784630:three_prime_UTR 45.0%
AACCCGAACTAAACCACGTT+GGG + chr5_2:69256350-69256369 Msa0784630:three_prime_UTR 45.0%
! TTCCCAACGTGGTTTAGTTC+GGG - chr5_2:69256355-69256374 None:intergenic 45.0%
!!! AGAAGGGTATTTTCCCAACG+TGG - chr5_2:69256366-69256385 None:intergenic 45.0%
AAGGAAACGAAGGGAAAGGA+AGG - chr5_2:69256460-69256479 None:intergenic 45.0%
AGTGAAGGAAACGAAGGGAA+AGG - chr5_2:69256464-69256483 None:intergenic 45.0%
TTTCCCTTCGTTTCCTTCAC+TGG + chr5_2:69256463-69256482 Msa0784630:three_prime_UTR 45.0%
AATGTGAAGTGAGCCAGTGA+AGG - chr5_2:69256479-69256498 None:intergenic 45.0%
AGGGTTATGCTAGGTTGCTT+GGG + chr5_2:69256625-69256644 Msa0784630:three_prime_UTR 45.0%
CTAGGTTGCTTGGGGAAAAT+GGG + chr5_2:69256634-69256653 Msa0784630:three_prime_UTR 45.0%
!! TGGCTTGTTTGAAAGTTGGG+AGG + chr5_2:69256742-69256761 Msa0784630:three_prime_UTR 45.0%
GAAAGTTGGGAGGACTGATT+TGG + chr5_2:69256752-69256771 Msa0784630:three_prime_UTR 45.0%
!! TTGGGAGGACTGATTTGGTT+TGG + chr5_2:69256757-69256776 Msa0784630:three_prime_UTR 45.0%
TGCGTATGAGCTTCTTAGAC+AGG + chr5_2:69256884-69256903 Msa0784630:three_prime_UTR 45.0%
CATCTCAAACCACAACTTCC+TGG - chr5_2:69257204-69257223 None:intergenic 45.0%
! TTCGTGATTTTGCAGAGGCA+AGG + chr5_2:69257288-69257307 Msa0784630:three_prime_UTR 45.0%
TCTGATTAAAGGCCTTTGCC+AGG + chr5_2:69257475-69257494 Msa0784630:three_prime_UTR 45.0%
ATTAAAGGCCTTTGCCAGGA+AGG + chr5_2:69257479-69257498 Msa0784630:three_prime_UTR 45.0%
TTGCAAAGTTGGAGACACTG+AGG + chr5_2:69257595-69257614 Msa0784630:three_prime_UTR 45.0%
TGCAAAGTTGGAGACACTGA+GGG + chr5_2:69257596-69257615 Msa0784630:three_prime_UTR 45.0%
GGTTGCTAAAGATGCTGAGT+TGG + chr5_2:69257735-69257754 Msa0784630:three_prime_UTR 45.0%
!!! TTTTAAATTACTTTTTTTTA+GGG - chr5_2:69255354-69255373 None:intergenic 5.0%
! TGATGTGAGAGCGCATCGAA+TGG - chr5_2:69254541-69254560 None:intergenic 50.0%
ACGGTCCCTAAGTGTCTGTT+TGG - chr5_2:69255035-69255054 None:intergenic 50.0%
ACAGACACTTAGGGACCGTT+TGG + chr5_2:69255036-69255055 Msa0784630:intron 50.0%
CACTTAGGGACCGTTTGGAT+TGG + chr5_2:69255041-69255060 Msa0784630:intron 50.0%
TGGCGGAGATGAATCACGAA+CGG - chr5_2:69255528-69255547 None:intergenic 50.0%
GTAGAAAATGGCGGTCATGC+AGG - chr5_2:69255554-69255573 None:intergenic 50.0%
AAACAGCTTATGGGCCCGTT+TGG - chr5_2:69255946-69255965 None:intergenic 50.0%
GGTGAGGTTTCGGAAATACG+AGG - chr5_2:69256259-69256278 None:intergenic 50.0%
GAGCCAGTGAAGGAAACGAA+GGG - chr5_2:69256469-69256488 None:intergenic 50.0%
TGAGCCAGTGAAGGAAACGA+AGG - chr5_2:69256470-69256489 None:intergenic 50.0%
!! CACTGGCTCACTTCACATTG+TGG + chr5_2:69256480-69256499 Msa0784630:three_prime_UTR 50.0%
GAGGGTTATGCTAGGTTGCT+TGG + chr5_2:69256624-69256643 Msa0784630:three_prime_UTR 50.0%
GGGTTATGCTAGGTTGCTTG+GGG + chr5_2:69256626-69256645 Msa0784630:three_prime_UTR 50.0%
GCTAGGTTGCTTGGGGAAAA+TGG + chr5_2:69256633-69256652 Msa0784630:three_prime_UTR 50.0%
GTTGCTTGGGGAAAATGGGA+TGG + chr5_2:69256638-69256657 Msa0784630:three_prime_UTR 50.0%
TGGGGAAAATGGGATGGTTG+AGG + chr5_2:69256644-69256663 Msa0784630:three_prime_UTR 50.0%
! TGATATGGGGTTGCTTGCTG+AGG + chr5_2:69257178-69257197 Msa0784630:three_prime_UTR 50.0%
AGCTCCTCCAAACACAAAGG+AGG - chr5_2:69257935-69257954 None:intergenic 50.0%
! TCGAATGGAAGAGAGTGCGC+AGG - chr5_2:69254526-69254545 None:intergenic 55.0%
TCTCTGTGCCCATGCTTCCA+TGG + chr5_2:69254688-69254707 Msa0784630:intron 55.0%
TGTGCCCATGCTTCCATGGT+TGG + chr5_2:69254692-69254711 Msa0784630:intron 55.0%
GGAGATGAATCACGAACGGG+CGG - chr5_2:69255524-69255543 None:intergenic 55.0%
GGCGGAGATGAATCACGAAC+GGG - chr5_2:69255527-69255546 None:intergenic 55.0%
! CGTGGCACCGAAGCTTCAAT+AGG - chr5_2:69256150-69256169 None:intergenic 55.0%
! TCGGATTGGAACAGCGTACG+TGG - chr5_2:69256168-69256187 None:intergenic 55.0%
TTGCTGAGGCCAGGAAGTTG+TGG + chr5_2:69257192-69257211 Msa0784630:three_prime_UTR 55.0%
CCTTAACTAGCTCGCCTTCC+TGG - chr5_2:69257496-69257515 None:intergenic 55.0%
CCAGGAAGGCGAGCTAGTTA+AGG + chr5_2:69257493-69257512 Msa0784630:three_prime_UTR 55.0%
GCGAGCTCCTCCAAACACAA+AGG - chr5_2:69257938-69257957 None:intergenic 55.0%
AGTGCGCAGGAGCTTGGCAA+TGG - chr5_2:69254513-69254532 None:intergenic 60.0%
! GAAGAGAGTGCGCAGGAGCT+TGG - chr5_2:69254519-69254538 None:intergenic 60.0%
GAATCACGAACGGGCGGAGA+GGG - chr5_2:69255518-69255537 None:intergenic 60.0%
TGAATCACGAACGGGCGGAG+AGG - chr5_2:69255519-69255538 None:intergenic 60.0%
AATGGCGGTCATGCAGGGAG+TGG - chr5_2:69255548-69255567 None:intergenic 60.0%
CTAGCTCGCCTTCCTGGCAA+AGG - chr5_2:69257490-69257509 None:intergenic 60.0%
GCGCAGGAGCTTGGCAATGG+CGG - chr5_2:69254510-69254529 None:intergenic 65.0%
! TGGGGTTGCTTGCTGAGGCC+AGG + chr5_2:69257183-69257202 Msa0784630:three_prime_UTR 65.0%
GGCGGTCATGCAGGGAGTGG+CGG - chr5_2:69255545-69255564 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 69254497 69258121 69254497 ID=Msa0784630;Name=Msa0784630
chr5_2 mRNA 69254497 69258121 69254497 ID=Msa0784630-mRNA-1;Parent=Msa0784630;Name=Msa0784630-mRNA-1;_AED=0.02;_eAED=0.02;_QI=48|0|1|1|0|0|2|2009|17
chr5_2 exon 69254497 69254621 69254497 ID=Msa0784630-mRNA-1:exon:20822;Parent=Msa0784630-mRNA-1
chr5_2 exon 69256136 69258121 69256136 ID=Msa0784630-mRNA-1:exon:20823;Parent=Msa0784630-mRNA-1
chr5_2 five_prime_UTR 69254497 69254544 69254497 ID=Msa0784630-mRNA-1:five_prime_utr;Parent=Msa0784630-mRNA-1
chr5_2 CDS 69254545 69254598 69254545 ID=Msa0784630-mRNA-1:cds;Parent=Msa0784630-mRNA-1
chr5_2 three_prime_UTR 69254599 69254621 69254599 ID=Msa0784630-mRNA-1:three_prime_utr;Parent=Msa0784630-mRNA-1
chr5_2 three_prime_UTR 69256136 69258121 69256136 ID=Msa0784630-mRNA-1:three_prime_utr;Parent=Msa0784630-mRNA-1
Gene Sequence

>Msa0784630

ATGCGCTCTCACATCATCTCTATCAGTTCCTCAATTTCTCTCCTCTTTCGCTGA
Protein sequence

>Msa0784630

MRSHIISISSSISLLFR*