AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0863810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 128 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATGATTATATGATAAT+TGG 0.136634 5_4:+63851227 Msa0863810:three_prime_UTR
TAGCTACAGCACAATGATTT+TGG 0.138385 5_4:+63850911 Msa0863810:three_prime_UTR
GCTAGGTTGCTTGGGGAAAA+TGG 0.147100 5_4:+63850186 Msa0863810:three_prime_UTR
GAAGCTTCAATAGGTTATTT+TGG 0.219887 5_4:-63849691 None:intergenic
GATAACTAAGAAAGAATTTC+GGG 0.237583 5_4:-63849969 None:intergenic
TGAGCTTCTTAGACAGGTTT+TGG 0.251317 5_4:+63850443 Msa0863810:three_prime_UTR
AGTTACAACTCTCTGATTAA+AGG 0.289233 5_4:+63851017 Msa0863810:three_prime_UTR
TAGAGGTTATGCAGAAAATT+TGG 0.294463 5_4:+63850887 Msa0863810:three_prime_UTR
TGCTCAAAAGTCTGCATATT+GGG 0.296680 5_4:-63851320 None:intergenic
AGGGTTATGCTAGGTTGCTT+GGG 0.297743 5_4:+63850178 Msa0863810:three_prime_UTR
AACTGTTGGGTGTTTGATTA+AGG 0.300973 5_4:+63850386 Msa0863810:three_prime_UTR
TTTAATGATCTTAAGGATAG+AGG 0.308650 5_4:+63850660 Msa0863810:three_prime_UTR
AGATAACTAAGAAAGAATTT+CGG 0.311149 5_4:-63849970 None:intergenic
GAGGGTTATGCTAGGTTGCT+TGG 0.315506 5_4:+63850177 Msa0863810:three_prime_UTR
ACTGTTTAAAACGAGAAAAT+AGG 0.320597 5_4:+63851351 Msa0863810:three_prime_UTR
ACGTGGTTTAGTTCGGGTTA+TGG 0.322164 5_4:-63849899 None:intergenic
CCTTAACTAGCTCGCCTTCC+TGG 0.330031 5_4:-63851046 None:intergenic
TATTGGCTTGTTTGAAAGTT+GGG 0.337316 5_4:+63850292 Msa0863810:three_prime_UTR
TTTCCCTTCGTTTCCTTCAC+TGG 0.337635 5_4:+63850016 Msa0863810:three_prime_UTR
TGTTCCGAAGAATGATTCTT+TGG 0.340309 5_4:+63850155 Msa0863810:three_prime_UTR
TTGGGAGGACTGATTTGGTT+TGG 0.346755 5_4:+63850310 Msa0863810:three_prime_UTR
TTTCCCAACGTGGTTTAGTT+CGG 0.347682 5_4:-63849906 None:intergenic
TTCCCAACGTGGTTTAGTTC+GGG 0.357239 5_4:-63849905 None:intergenic
TTGATCTTTAGGATCTTGTT+TGG 0.358934 5_4:-63849845 None:intergenic
CCAAACACAAAGGAGGAAAA+AGG 0.359317 5_4:-63851478 None:intergenic
ACTTATCAAGAGATTATTCA+TGG 0.363261 5_4:+63850597 Msa0863810:three_prime_UTR
TTGTTTCTGTGGTGAGGTTT+CGG 0.369531 5_4:-63849819 None:intergenic
CATCTCAAACCACAACTTCC+TGG 0.372583 5_4:-63850754 None:intergenic
ACGTATAATGTAATGATATA+TGG 0.376012 5_4:+63850807 Msa0863810:three_prime_UTR
GAAAGTTGGGAGGACTGATT+TGG 0.377688 5_4:+63850305 Msa0863810:three_prime_UTR
TATGAGCATATGATTGAATC+TGG 0.380517 5_4:+63850339 Msa0863810:three_prime_UTR
TTTGATGCTCTTGTTGATGC+TGG 0.396467 5_4:+63850090 Msa0863810:three_prime_UTR
ATAAAATGTCTTTGCAAAGT+TGG 0.397144 5_4:+63851137 Msa0863810:three_prime_UTR
ATATGATTGAATCTGGGGTT+GGG 0.403623 5_4:+63850346 Msa0863810:three_prime_UTR
CACATGATTATATGATAATT+GGG 0.407028 5_4:+63851228 Msa0863810:three_prime_UTR
TCATTCTTCGGAACAAAATC+AGG 0.417363 5_4:-63850147 None:intergenic
TGGTTTGAGATGATTCAAAA+AGG 0.417493 5_4:+63850765 Msa0863810:three_prime_UTR
GTGCTCAAAAGTCTGCATAT+TGG 0.418414 5_4:-63851321 None:intergenic
AATGATATATGGTTATTGTA+AGG 0.426559 5_4:+63850818 Msa0863810:three_prime_UTR
CGTGGCACCGAAGCTTCAAT+AGG 0.432572 5_4:-63849700 None:intergenic
TTCACATTGTGGTTTCAAAC+CGG 0.433912 5_4:+63850044 Msa0863810:three_prime_UTR
CCAGGAAGGCGAGCTAGTTA+AGG 0.434932 5_4:+63851046 Msa0863810:three_prime_UTR
AGATATGCATGATAGGCATT+TGG 0.437930 5_4:+63851190 Msa0863810:three_prime_UTR
AAAATAACCTATTGAAGCTT+CGG 0.439177 5_4:+63849693 Msa0863810:three_prime_UTR
TTTGTTAGTTTGAAAGAGGT+TGG 0.442556 5_4:+63850234 Msa0863810:three_prime_UTR
TAATCATGTGTACTAGCAAT+TGG 0.446374 5_4:-63851215 None:intergenic
TCTGAGTGACATTGTCATCA+TGG 0.446532 5_4:-63849776 None:intergenic
TGCGTATGAGCTTCTTAGAC+AGG 0.446776 5_4:+63850437 Msa0863810:three_prime_UTR
ATTCAACGAACTTCTTATGC+AGG 0.447523 5_4:+63851079 Msa0863810:three_prime_UTR
ACATTATACGTGTACTCATT+CGG 0.449189 5_4:-63850795 None:intergenic
AACCCGAACTAAACCACGTT+GGG 0.449791 5_4:+63849903 Msa0863810:three_prime_UTR
CTAGGTTGCTTGGGGAAAAT+GGG 0.452180 5_4:+63850187 Msa0863810:three_prime_UTR
CACTGGCTCACTTCACATTG+TGG 0.454789 5_4:+63850033 Msa0863810:three_prime_UTR
TTATTGGCTTGTTTGAAAGT+TGG 0.455824 5_4:+63850291 Msa0863810:three_prime_UTR
TTCAAAGGTTGTTGTCGGAT+TGG 0.457536 5_4:-63849732 None:intergenic
TATGGTGCAGATTTCGTTGA+GGG 0.462402 5_4:-63849881 None:intergenic
CCCTCCAAAGAATCATTCTT+CGG 0.464196 5_4:-63850159 None:intergenic
AATATTGATGTTGAAACTGT+TGG 0.465621 5_4:+63850372 Msa0863810:three_prime_UTR
GTTGTATACATAACCCTGTC+TGG 0.470369 5_4:-63850690 None:intergenic
TGTTGAAAGATATGCATGAT+AGG 0.470710 5_4:+63851183 Msa0863810:three_prime_UTR
ACAGTTTCAAAGGTTGTTGT+CGG 0.475154 5_4:-63849737 None:intergenic
GAAGAGAGTGCGCAGGAGCT+TGG 0.478717 5_4:-63848062 None:intergenic
TAGATTTAATGTTTAGAGAA+GGG 0.480778 5_4:+63851396 Msa0863810:three_prime_UTR
GGTTTGAGAAATTGAAAGAT+GGG 0.486122 5_4:-63849948 None:intergenic
TGGGGTTGCTTGCTGAGGCC+AGG 0.487470 5_4:+63850736 Msa0863810:three_prime_UTR
ACAACTTGAAGGTTTACAAA+GGG 0.488087 5_4:-63851626 None:intergenic
GCATTATATGAAGATTGATC+CGG 0.493959 5_4:-63850063 None:intergenic
GCGTACATATAACAACTTGA+AGG 0.495793 5_4:-63851637 None:intergenic
CCGAAGAATGATTCTTTGGA+GGG 0.496823 5_4:+63850159 Msa0863810:three_prime_UTR
CTGAGTGACATTGTCATCAT+GGG 0.497507 5_4:-63849775 None:intergenic
TGGGGAAAATGGGATGGTTG+AGG 0.498283 5_4:+63850197 Msa0863810:three_prime_UTR
TCTGATTAAAGGCCTTTGCC+AGG 0.500511 5_4:+63851028 Msa0863810:three_prime_UTR
CATATGATTGAATCTGGGGT+TGG 0.502079 5_4:+63850345 Msa0863810:three_prime_UTR
GGGTTTGAGAAATTGAAAGA+TGG 0.502373 5_4:-63849949 None:intergenic
ATATTGATGTTGAAACTGTT+GGG 0.505327 5_4:+63850373 Msa0863810:three_prime_UTR
TTAGATTTAATGTTTAGAGA+AGG 0.511904 5_4:+63851395 Msa0863810:three_prime_UTR
TATGATTGAATCTGGGGTTG+GGG 0.514223 5_4:+63850347 Msa0863810:three_prime_UTR
TTAATGATCTTAAGGATAGA+GGG 0.516349 5_4:+63850661 Msa0863810:three_prime_UTR
AAGATGGGTATTGTGAAAGA+AGG 0.516570 5_4:-63849933 None:intergenic
TTGTTTGGTTGTTGTTTCTG+TGG 0.518610 5_4:-63849830 None:intergenic
TGTGTTTGTTAGTTTGAAAG+AGG 0.519159 5_4:+63850230 Msa0863810:three_prime_UTR
GCGAAAGAGGAGAGAAATTG+AGG 0.524835 5_4:-63848119 None:intergenic
TCCGAAGAATGATTCTTTGG+AGG 0.528081 5_4:+63850158 Msa0863810:three_prime_UTR
ATGTATACAACGGTTATCAA+AGG 0.530143 5_4:+63850702 Msa0863810:three_prime_UTR
TGGTTGTTGTTTCTGTGGTG+AGG 0.532400 5_4:-63849825 None:intergenic
AAGGAAACGAAGGGAAAGGA+AGG 0.535168 5_4:-63850010 None:intergenic
GCGCAGGAGCTTGGCAATGG+CGG 0.537744 5_4:-63848053 None:intergenic
TGATGTGAGAGCGCATCGAA+TGG 0.539610 5_4:-63848084 None:intergenic
TGTGAATGAAACAGTTTCAA+AGG 0.543255 5_4:-63849747 None:intergenic
TTTCATGAAATGTCGCGAAA+GGG 0.544857 5_4:+63850975 Msa0863810:three_prime_UTR
AATCATCATTTAATAGTACA+TGG 0.545178 5_4:-63851550 None:intergenic
AGTGCGCAGGAGCTTGGCAA+TGG 0.547796 5_4:-63848056 None:intergenic
TTCAACGAACTTCTTATGCA+GGG 0.548937 5_4:+63851080 Msa0863810:three_prime_UTR
AGATGGGTATTGTGAAAGAA+GGG 0.552728 5_4:-63849932 None:intergenic
ATTCTTTGGAGGGTTATGCT+AGG 0.552937 5_4:+63850169 Msa0863810:three_prime_UTR
ATTAAAGGCCTTTGCCAGGA+AGG 0.553517 5_4:+63851032 Msa0863810:three_prime_UTR
GGTTGCTAAAGATGCTGAGT+TGG 0.557716 5_4:+63851288 Msa0863810:three_prime_UTR
GAAGAGGAAGAAGAATGATG+AGG 0.559419 5_4:+63850626 Msa0863810:three_prime_UTR
TTGCTGAGGCCAGGAAGTTG+TGG 0.561607 5_4:+63850745 Msa0863810:three_prime_UTR
GTTTCATGAAATGTCGCGAA+AGG 0.562910 5_4:+63850974 Msa0863810:three_prime_UTR
CTAGCTCGCCTTCCTGGCAA+AGG 0.572227 5_4:-63851040 None:intergenic
GGGTTATGCTAGGTTGCTTG+GGG 0.575307 5_4:+63850179 Msa0863810:three_prime_UTR
TATGATGATATGTGTGGTAG+AGG 0.577409 5_4:+63850870 Msa0863810:three_prime_UTR
TGATATGGGGTTGCTTGCTG+AGG 0.578172 5_4:+63850731 Msa0863810:three_prime_UTR
TGCAAAGTTGGAGACACTGA+GGG 0.588567 5_4:+63851149 Msa0863810:three_prime_UTR
AGTGAAGGAAACGAAGGGAA+AGG 0.592056 5_4:-63850014 None:intergenic
AACAACTTGAAGGTTTACAA+AGG 0.592729 5_4:-63851627 None:intergenic
TTATGGTGCAGATTTCGTTG+AGG 0.598996 5_4:-63849882 None:intergenic
AAGAAAATTACCTTGAACTG+CGG 0.604829 5_4:-63848156 None:intergenic
ATGAGCATATGATTGAATCT+GGG 0.609681 5_4:+63850340 Msa0863810:three_prime_UTR
TGGCTTGTTTGAAAGTTGGG+AGG 0.614610 5_4:+63850295 Msa0863810:three_prime_UTR
GGAGTAAAGGAAGAAACAGA+TGG 0.618721 5_4:-63851110 None:intergenic
TCTTGATAAGTATAGATACT+CGG 0.625160 5_4:-63850585 None:intergenic
GTTGCTTGGGGAAAATGGGA+TGG 0.629510 5_4:+63850191 Msa0863810:three_prime_UTR
AATGTGAAGTGAGCCAGTGA+AGG 0.629610 5_4:-63850029 None:intergenic
TCGAATGGAAGAGAGTGCGC+AGG 0.633972 5_4:-63848069 None:intergenic
TGAGCCAGTGAAGGAAACGA+AGG 0.636495 5_4:-63850020 None:intergenic
TCGGATTGGAACAGCGTACG+TGG 0.642357 5_4:-63849718 None:intergenic
TAACCCGAACTAAACCACGT+TGG 0.647364 5_4:+63849902 Msa0863810:three_prime_UTR
ACATTAAAACTCAACACAGA+CGG 0.654839 5_4:-63850264 None:intergenic
GAGCCAGTGAAGGAAACGAA+GGG 0.656330 5_4:-63850019 None:intergenic
GCGAGCTCCTCCAAACACAA+AGG 0.670155 5_4:-63851488 None:intergenic
TGAGCATATGATTGAATCTG+GGG 0.670828 5_4:+63850341 Msa0863810:three_prime_UTR
GGTGAGGTTTCGGAAATACG+AGG 0.677848 5_4:-63849809 None:intergenic
AGCTCCTCCAAACACAAAGG+AGG 0.685602 5_4:-63851485 None:intergenic
AGACAGGGTTATGTATACAA+CGG 0.685868 5_4:+63850692 Msa0863810:three_prime_UTR
TTGCAAAGTTGGAGACACTG+AGG 0.690667 5_4:+63851148 Msa0863810:three_prime_UTR
AAGCTCTATGATGATATGTG+TGG 0.757559 5_4:+63850864 Msa0863810:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTTTTTTTTTT+TGG + chr5_4:63851570-63851589 Msa0863810:three_prime_UTR 0.0%
!!! TTTTTTTTTTTTTTTTTTTT+GGG + chr5_4:63851571-63851590 Msa0863810:three_prime_UTR 0.0%
!! AAATCTTTCAAAAAAAAAAA+TGG - chr5_4:63848827-63848846 None:intergenic 10.0%
!! TAATTCAAGTAATTAAAAAA+TGG - chr5_4:63848855-63848874 None:intergenic 10.0%
!! CTTAATTAAAATAATTAAGT+TGG - chr5_4:63848938-63848957 None:intergenic 10.0%
!!! TTTTCAAATAAAATAAGAAT+TGG - chr5_4:63849629-63849648 None:intergenic 10.0%
!!! TAAATTTTGTAAGATTGTTT+GGG - chr5_4:63848529-63848548 None:intergenic 15.0%
!!! ATAAATTTTGTAAGATTGTT+TGG - chr5_4:63848530-63848549 None:intergenic 15.0%
!!! GATATTGATTATATTTTTTG+AGG + chr5_4:63848763-63848782 Msa0863810:intron 15.0%
!! ATTACAAGTTATAACTTACA+GGG - chr5_4:63848420-63848439 None:intergenic 20.0%
!! AATTACAAGTTATAACTTAC+AGG - chr5_4:63848421-63848440 None:intergenic 20.0%
!! TATTATTCATAAGTTTGTCA+AGG + chr5_4:63848648-63848667 Msa0863810:intron 20.0%
!! CTTTCAAAAAAAAAAATGGA+TGG - chr5_4:63848823-63848842 None:intergenic 20.0%
!! TCAAGTAATTAAAAAATGGA+TGG - chr5_4:63848851-63848870 None:intergenic 20.0%
!! AGATAACTAAGAAAGAATTT+CGG - chr5_4:63849973-63849992 None:intergenic 20.0%
!!! TAGAGTTTTTAATGATCTTA+AGG + chr5_4:63850653-63850672 Msa0863810:three_prime_UTR 20.0%
!! ACGTATAATGTAATGATATA+TGG + chr5_4:63850807-63850826 Msa0863810:three_prime_UTR 20.0%
!! AATGATATATGGTTATTGTA+AGG + chr5_4:63850818-63850837 Msa0863810:three_prime_UTR 20.0%
!! ACACATGATTATATGATAAT+TGG + chr5_4:63851227-63851246 Msa0863810:three_prime_UTR 20.0%
!! CACATGATTATATGATAATT+GGG + chr5_4:63851228-63851247 Msa0863810:three_prime_UTR 20.0%
!! ATAATTGGGTTAAGTAAAAA+AGG + chr5_4:63851242-63851261 Msa0863810:three_prime_UTR 20.0%
!! TTAGATTTAATGTTTAGAGA+AGG + chr5_4:63851395-63851414 Msa0863810:three_prime_UTR 20.0%
!! TAGATTTAATGTTTAGAGAA+GGG + chr5_4:63851396-63851415 Msa0863810:three_prime_UTR 20.0%
!! AATCATCATTTAATAGTACA+TGG - chr5_4:63851553-63851572 None:intergenic 20.0%
!! TCATCTTTTCAATTTTCATC+TGG + chr5_4:63848190-63848209 Msa0863810:intron 25.0%
! AAAAAATGCTCAAAAGAATG+TGG - chr5_4:63848291-63848310 None:intergenic 25.0%
! AAAAAATAAGCCAATCCAAA+CGG - chr5_4:63848600-63848619 None:intergenic 25.0%
!! TTTTCTTAAGACTCGTATTA+AGG + chr5_4:63848975-63848994 Msa0863810:intron 25.0%
! CATGTTTCAAAGTTAGAATT+GGG - chr5_4:63849001-63849020 None:intergenic 25.0%
! ACATGTTTCAAAGTTAGAAT+TGG - chr5_4:63849002-63849021 None:intergenic 25.0%
!!! AAATCTTACTTTTGTTTGAG+AGG - chr5_4:63849129-63849148 None:intergenic 25.0%
!! TACAAGTTTTCTTCTACTAA+GGG - chr5_4:63849546-63849565 None:intergenic 25.0%
!! ATACAAGTTTTCTTCTACTA+AGG - chr5_4:63849547-63849566 None:intergenic 25.0%
! AAAATAACCTATTGAAGCTT+CGG + chr5_4:63849693-63849712 Msa0863810:three_prime_UTR 25.0%
!!! TGAGTAAAGTTTTGATCTTT+AGG - chr5_4:63849859-63849878 None:intergenic 25.0%
! GATAACTAAGAAAGAATTTC+GGG - chr5_4:63849972-63849991 None:intergenic 25.0%
!!! GAGTTTTAATGTGTGTTTAT+TGG + chr5_4:63850275-63850294 Msa0863810:three_prime_UTR 25.0%
! AATATTGATGTTGAAACTGT+TGG + chr5_4:63850372-63850391 Msa0863810:three_prime_UTR 25.0%
! ATATTGATGTTGAAACTGTT+GGG + chr5_4:63850373-63850392 Msa0863810:three_prime_UTR 25.0%
! TCTTGATAAGTATAGATACT+CGG - chr5_4:63850588-63850607 None:intergenic 25.0%
! ACTTATCAAGAGATTATTCA+TGG + chr5_4:63850597-63850616 Msa0863810:three_prime_UTR 25.0%
! TTTAATGATCTTAAGGATAG+AGG + chr5_4:63850660-63850679 Msa0863810:three_prime_UTR 25.0%
! TTAATGATCTTAAGGATAGA+GGG + chr5_4:63850661-63850680 Msa0863810:three_prime_UTR 25.0%
!! TATCAAAGGTTTTTGTGATA+TGG + chr5_4:63850716-63850735 Msa0863810:three_prime_UTR 25.0%
!! ATCAAAGGTTTTTGTGATAT+GGG + chr5_4:63850717-63850736 Msa0863810:three_prime_UTR 25.0%
! ATAAAATGTCTTTGCAAAGT+TGG + chr5_4:63851137-63851156 Msa0863810:three_prime_UTR 25.0%
!! ACTGTTTAAAACGAGAAAAT+AGG + chr5_4:63851351-63851370 Msa0863810:three_prime_UTR 25.0%
!! AAAAAGCAGAATTTGTTCTT+TGG + chr5_4:63851430-63851449 Msa0863810:three_prime_UTR 25.0%
AAGAAAATTACCTTGAACTG+CGG - chr5_4:63848159-63848178 None:intergenic 30.0%
TTAATCCAAACAGACACTTA+GGG + chr5_4:63848573-63848592 Msa0863810:intron 30.0%
!! TTTTGTCACTTGATGTTCTA+TGG - chr5_4:63849033-63849052 None:intergenic 30.0%
TTTGTTTGAGAGGTAGAAAA+TGG - chr5_4:63849119-63849138 None:intergenic 30.0%
ATAAGCTGTTTATCCAAACA+GGG - chr5_4:63849238-63849257 None:intergenic 30.0%
TATAAGCTGTTTATCCAAAC+AGG - chr5_4:63849239-63849258 None:intergenic 30.0%
TTACCAAGATAAGCTATAAG+CGG - chr5_4:63849279-63849298 None:intergenic 30.0%
AGAAGAAAACTTGTATCACA+TGG + chr5_4:63849551-63849570 Msa0863810:intron 30.0%
! GAAGCTTCAATAGGTTATTT+TGG - chr5_4:63849694-63849713 None:intergenic 30.0%
TGTGAATGAAACAGTTTCAA+AGG - chr5_4:63849750-63849769 None:intergenic 30.0%
!! TTGATCTTTAGGATCTTGTT+TGG - chr5_4:63849848-63849867 None:intergenic 30.0%
GGTTTGAGAAATTGAAAGAT+GGG - chr5_4:63849951-63849970 None:intergenic 30.0%
GCATTATATGAAGATTGATC+CGG - chr5_4:63850066-63850085 None:intergenic 30.0%
! TGTGTTTGTTAGTTTGAAAG+AGG + chr5_4:63850230-63850249 Msa0863810:three_prime_UTR 30.0%
! TTTGTTAGTTTGAAAGAGGT+TGG + chr5_4:63850234-63850253 Msa0863810:three_prime_UTR 30.0%
ACATTAAAACTCAACACAGA+CGG - chr5_4:63850267-63850286 None:intergenic 30.0%
!! TTATTGGCTTGTTTGAAAGT+TGG + chr5_4:63850291-63850310 Msa0863810:three_prime_UTR 30.0%
!! TATTGGCTTGTTTGAAAGTT+GGG + chr5_4:63850292-63850311 Msa0863810:three_prime_UTR 30.0%
TATGAGCATATGATTGAATC+TGG + chr5_4:63850339-63850358 Msa0863810:three_prime_UTR 30.0%
ATGAGCATATGATTGAATCT+GGG + chr5_4:63850340-63850359 Msa0863810:three_prime_UTR 30.0%
!! TTATTCATGGACTTTTGAAG+AGG + chr5_4:63850610-63850629 Msa0863810:three_prime_UTR 30.0%
ATGTATACAACGGTTATCAA+AGG + chr5_4:63850702-63850721 Msa0863810:three_prime_UTR 30.0%
! TCAAAGGTTTTTGTGATATG+GGG + chr5_4:63850718-63850737 Msa0863810:three_prime_UTR 30.0%
! TGGTTTGAGATGATTCAAAA+AGG + chr5_4:63850765-63850784 Msa0863810:three_prime_UTR 30.0%
ACATTATACGTGTACTCATT+CGG - chr5_4:63850798-63850817 None:intergenic 30.0%
TAGAGGTTATGCAGAAAATT+TGG + chr5_4:63850887-63850906 Msa0863810:three_prime_UTR 30.0%
AGTTACAACTCTCTGATTAA+AGG + chr5_4:63851017-63851036 Msa0863810:three_prime_UTR 30.0%
! ACATTTTATGAGAGGAGTAA+AGG - chr5_4:63851126-63851145 None:intergenic 30.0%
! TTGCAAAGACATTTTATGAG+AGG - chr5_4:63851134-63851153 None:intergenic 30.0%
TGTTGAAAGATATGCATGAT+AGG + chr5_4:63851183-63851202 Msa0863810:three_prime_UTR 30.0%
TAATCATGTGTACTAGCAAT+TGG - chr5_4:63851218-63851237 None:intergenic 30.0%
! AAAAAAGGAGATTTTGCACA+AGG + chr5_4:63851257-63851276 Msa0863810:three_prime_UTR 30.0%
ACAACTTGAAGGTTTACAAA+GGG - chr5_4:63851629-63851648 None:intergenic 30.0%
AACAACTTGAAGGTTTACAA+AGG - chr5_4:63851630-63851649 None:intergenic 30.0%
GTGGCATATGTCATATGAAA+CGG - chr5_4:63848272-63848291 None:intergenic 35.0%
GTTAATCCAAACAGACACTT+AGG + chr5_4:63848572-63848591 Msa0863810:intron 35.0%
AAGTGACAAAACTATGCGTT+GGG + chr5_4:63849042-63849061 Msa0863810:intron 35.0%
AGGGTCAAAATCAAAGAACA+GGG - chr5_4:63849219-63849238 None:intergenic 35.0%
!! TCTTTGATTTTGACCCTGTT+TGG + chr5_4:63849222-63849241 Msa0863810:intron 35.0%
CACAAGTAAGTCAATCCAAA+CGG + chr5_4:63849481-63849500 Msa0863810:intron 35.0%
ACAAGTAAGTCAATCCAAAC+GGG + chr5_4:63849482-63849501 Msa0863810:intron 35.0%
AGAACTCTCAAACAGCTTAT+GGG - chr5_4:63849508-63849527 None:intergenic 35.0%
AAGAACTCTCAAACAGCTTA+TGG - chr5_4:63849509-63849528 None:intergenic 35.0%
AAGCTGTTTGAGAGTTCTTA+TGG + chr5_4:63849510-63849529 Msa0863810:intron 35.0%
TGTTTGAGAGTTCTTATGGA+TGG + chr5_4:63849514-63849533 Msa0863810:intron 35.0%
TATCACATGGAAATAGAGCA+AGG + chr5_4:63849564-63849583 Msa0863810:intron 35.0%
ATCACATGGAAATAGAGCAA+GGG + chr5_4:63849565-63849584 Msa0863810:intron 35.0%
ACAGTTTCAAAGGTTGTTGT+CGG - chr5_4:63849740-63849759 None:intergenic 35.0%
!! TTGTTTGGTTGTTGTTTCTG+TGG - chr5_4:63849833-63849852 None:intergenic 35.0%
! AGATGGGTATTGTGAAAGAA+GGG - chr5_4:63849935-63849954 None:intergenic 35.0%
! AAGATGGGTATTGTGAAAGA+AGG - chr5_4:63849936-63849955 None:intergenic 35.0%
GGGTTTGAGAAATTGAAAGA+TGG - chr5_4:63849952-63849971 None:intergenic 35.0%
TTCACATTGTGGTTTCAAAC+CGG + chr5_4:63850044-63850063 Msa0863810:three_prime_UTR 35.0%
TCATTCTTCGGAACAAAATC+AGG - chr5_4:63850150-63850169 None:intergenic 35.0%
TGTTCCGAAGAATGATTCTT+TGG + chr5_4:63850155-63850174 Msa0863810:three_prime_UTR 35.0%
TGAGCATATGATTGAATCTG+GGG + chr5_4:63850341-63850360 Msa0863810:three_prime_UTR 35.0%
! ATATGATTGAATCTGGGGTT+GGG + chr5_4:63850346-63850365 Msa0863810:three_prime_UTR 35.0%
AACTGTTGGGTGTTTGATTA+AGG + chr5_4:63850386-63850405 Msa0863810:three_prime_UTR 35.0%
!! TTTGATTAAGGCGTTTTGTG+CGG + chr5_4:63850398-63850417 Msa0863810:three_prime_UTR 35.0%
! GTTTTTAACGCGCTTATAAC+TGG + chr5_4:63850492-63850511 Msa0863810:three_prime_UTR 35.0%
AGACAGGGTTATGTATACAA+CGG + chr5_4:63850692-63850711 Msa0863810:three_prime_UTR 35.0%
AAGCTCTATGATGATATGTG+TGG + chr5_4:63850864-63850883 Msa0863810:three_prime_UTR 35.0%
TATGATGATATGTGTGGTAG+AGG + chr5_4:63850870-63850889 Msa0863810:three_prime_UTR 35.0%
! TAGCTACAGCACAATGATTT+TGG + chr5_4:63850911-63850930 Msa0863810:three_prime_UTR 35.0%
! AGCTACAGCACAATGATTTT+GGG + chr5_4:63850912-63850931 Msa0863810:three_prime_UTR 35.0%
TTTCATGAAATGTCGCGAAA+GGG + chr5_4:63850975-63850994 Msa0863810:three_prime_UTR 35.0%
ATTCAACGAACTTCTTATGC+AGG + chr5_4:63851079-63851098 Msa0863810:three_prime_UTR 35.0%
TTCAACGAACTTCTTATGCA+GGG + chr5_4:63851080-63851099 Msa0863810:three_prime_UTR 35.0%
AGATATGCATGATAGGCATT+TGG + chr5_4:63851190-63851209 Msa0863810:three_prime_UTR 35.0%
! ATTTTGCACAAGGTATGGAA+TGG + chr5_4:63851267-63851286 Msa0863810:three_prime_UTR 35.0%
TGCTCAAAAGTCTGCATATT+GGG - chr5_4:63851323-63851342 None:intergenic 35.0%
!! CTCGTTTTAAACAGTCAATC+AGG - chr5_4:63851346-63851365 None:intergenic 35.0%
GCGTACATATAACAACTTGA+AGG - chr5_4:63851640-63851659 None:intergenic 35.0%
! AAAAACGGATTCAGCGAAAG+AGG - chr5_4:63848135-63848154 None:intergenic 40.0%
ATGGGCACAGAGAGAAAAAT+CGG - chr5_4:63848227-63848246 None:intergenic 40.0%
AAAATATGTCCTCGTGAGCT+TGG - chr5_4:63848689-63848708 None:intergenic 40.0%
CAAGTGACAAAACTATGCGT+TGG + chr5_4:63849041-63849060 Msa0863810:intron 40.0%
GTTTGAGAGGTAGAAAATGG+CGG - chr5_4:63849116-63849135 None:intergenic 40.0%
CAGGGTCAAAATCAAAGAAC+AGG - chr5_4:63849220-63849239 None:intergenic 40.0%
TTCAAAGGTTGTTGTCGGAT+TGG - chr5_4:63849735-63849754 None:intergenic 40.0%
CTGAGTGACATTGTCATCAT+GGG - chr5_4:63849778-63849797 None:intergenic 40.0%
TCTGAGTGACATTGTCATCA+TGG - chr5_4:63849779-63849798 None:intergenic 40.0%
!! TTGTTTCTGTGGTGAGGTTT+CGG - chr5_4:63849822-63849841 None:intergenic 40.0%
TATGGTGCAGATTTCGTTGA+GGG - chr5_4:63849884-63849903 None:intergenic 40.0%
TTATGGTGCAGATTTCGTTG+AGG - chr5_4:63849885-63849904 None:intergenic 40.0%
TTTCCCAACGTGGTTTAGTT+CGG - chr5_4:63849909-63849928 None:intergenic 40.0%
TTTGATGCTCTTGTTGATGC+TGG + chr5_4:63850090-63850109 Msa0863810:three_prime_UTR 40.0%
TCCGAAGAATGATTCTTTGG+AGG + chr5_4:63850158-63850177 Msa0863810:three_prime_UTR 40.0%
CCCTCCAAAGAATCATTCTT+CGG - chr5_4:63850162-63850181 None:intergenic 40.0%
CCGAAGAATGATTCTTTGGA+GGG + chr5_4:63850159-63850178 Msa0863810:three_prime_UTR 40.0%
! ATTCTTTGGAGGGTTATGCT+AGG + chr5_4:63850169-63850188 Msa0863810:three_prime_UTR 40.0%
! CATATGATTGAATCTGGGGT+TGG + chr5_4:63850345-63850364 Msa0863810:three_prime_UTR 40.0%
! TATGATTGAATCTGGGGTTG+GGG + chr5_4:63850347-63850366 Msa0863810:three_prime_UTR 40.0%
! TGAGCTTCTTAGACAGGTTT+TGG + chr5_4:63850443-63850462 Msa0863810:three_prime_UTR 40.0%
!!! TCTTAGACAGGTTTTGGAGA+AGG + chr5_4:63850449-63850468 Msa0863810:three_prime_UTR 40.0%
!!! CTTAGACAGGTTTTGGAGAA+GGG + chr5_4:63850450-63850469 Msa0863810:three_prime_UTR 40.0%
GAAGAGGAAGAAGAATGATG+AGG + chr5_4:63850626-63850645 Msa0863810:three_prime_UTR 40.0%
!!! ATAGAGGGTATTTTCCAGAC+AGG + chr5_4:63850676-63850695 Msa0863810:three_prime_UTR 40.0%
!!! TAGAGGGTATTTTCCAGACA+GGG + chr5_4:63850677-63850696 Msa0863810:three_prime_UTR 40.0%
GTTGTATACATAACCCTGTC+TGG - chr5_4:63850693-63850712 None:intergenic 40.0%
! TAAGGTTCGTGATTTTGCAG+AGG + chr5_4:63850836-63850855 Msa0863810:three_prime_UTR 40.0%
!! ATTTTGGGTCTGTGTTTGCA+TGG + chr5_4:63850927-63850946 Msa0863810:three_prime_UTR 40.0%
GTTTCATGAAATGTCGCGAA+AGG + chr5_4:63850974-63850993 Msa0863810:three_prime_UTR 40.0%
GGAGTAAAGGAAGAAACAGA+TGG - chr5_4:63851113-63851132 None:intergenic 40.0%
! AGGAGATTTTGCACAAGGTA+TGG + chr5_4:63851262-63851281 Msa0863810:three_prime_UTR 40.0%
GTGCTCAAAAGTCTGCATAT+TGG - chr5_4:63851324-63851343 None:intergenic 40.0%
CCAAACACAAAGGAGGAAAA+AGG - chr5_4:63851481-63851500 None:intergenic 40.0%
! CCTTTTTCCTCCTTTGTGTT+TGG + chr5_4:63851478-63851497 Msa0863810:three_prime_UTR 40.0%
! TTTTCCTCCTTTGTGTTTGG+AGG + chr5_4:63851481-63851500 Msa0863810:three_prime_UTR 40.0%
GCGAAAGAGGAGAGAAATTG+AGG - chr5_4:63848122-63848141 None:intergenic 45.0%
ACCTTGAACTGCGGCAAAAA+CGG - chr5_4:63848150-63848169 None:intergenic 45.0%
! AATGCCAACCATGGAAGCAT+GGG - chr5_4:63848245-63848264 None:intergenic 45.0%
TAATGCCAACCATGGAAGCA+TGG - chr5_4:63848246-63848265 None:intergenic 45.0%
AACGGAAGTAATGCCAACCA+TGG - chr5_4:63848254-63848273 None:intergenic 45.0%
ATGAAGATACCAAGCTCACG+AGG + chr5_4:63848677-63848696 Msa0863810:intron 45.0%
TAGAAAATGGCGGTCATGCA+GGG - chr5_4:63849106-63849125 None:intergenic 45.0%
CAGGGTGATGAGAATTAAGC+AGG - chr5_4:63849201-63849220 None:intergenic 45.0%
CTATAAGCGGCTGTGCTATA+AGG - chr5_4:63849266-63849285 None:intergenic 45.0%
CAGCCGCTTATAGCTTATCT+TGG + chr5_4:63849273-63849292 Msa0863810:intron 45.0%
TGGAAATAGAGCAAGGGCAA+TGG + chr5_4:63849571-63849590 Msa0863810:intron 45.0%
!! TGGTTGTTGTTTCTGTGGTG+AGG - chr5_4:63849828-63849847 None:intergenic 45.0%
! ACGTGGTTTAGTTCGGGTTA+TGG - chr5_4:63849902-63849921 None:intergenic 45.0%
TAACCCGAACTAAACCACGT+TGG + chr5_4:63849902-63849921 Msa0863810:three_prime_UTR 45.0%
AACCCGAACTAAACCACGTT+GGG + chr5_4:63849903-63849922 Msa0863810:three_prime_UTR 45.0%
! TTCCCAACGTGGTTTAGTTC+GGG - chr5_4:63849908-63849927 None:intergenic 45.0%
!!! AGAAGGGTATTTTCCCAACG+TGG - chr5_4:63849919-63849938 None:intergenic 45.0%
AAGGAAACGAAGGGAAAGGA+AGG - chr5_4:63850013-63850032 None:intergenic 45.0%
AGTGAAGGAAACGAAGGGAA+AGG - chr5_4:63850017-63850036 None:intergenic 45.0%
TTTCCCTTCGTTTCCTTCAC+TGG + chr5_4:63850016-63850035 Msa0863810:three_prime_UTR 45.0%
AATGTGAAGTGAGCCAGTGA+AGG - chr5_4:63850032-63850051 None:intergenic 45.0%
AGGGTTATGCTAGGTTGCTT+GGG + chr5_4:63850178-63850197 Msa0863810:three_prime_UTR 45.0%
CTAGGTTGCTTGGGGAAAAT+GGG + chr5_4:63850187-63850206 Msa0863810:three_prime_UTR 45.0%
!! TGGCTTGTTTGAAAGTTGGG+AGG + chr5_4:63850295-63850314 Msa0863810:three_prime_UTR 45.0%
GAAAGTTGGGAGGACTGATT+TGG + chr5_4:63850305-63850324 Msa0863810:three_prime_UTR 45.0%
!! TTGGGAGGACTGATTTGGTT+TGG + chr5_4:63850310-63850329 Msa0863810:three_prime_UTR 45.0%
TGCGTATGAGCTTCTTAGAC+AGG + chr5_4:63850437-63850456 Msa0863810:three_prime_UTR 45.0%
CATCTCAAACCACAACTTCC+TGG - chr5_4:63850757-63850776 None:intergenic 45.0%
! TTCGTGATTTTGCAGAGGCA+AGG + chr5_4:63850841-63850860 Msa0863810:three_prime_UTR 45.0%
TCTGATTAAAGGCCTTTGCC+AGG + chr5_4:63851028-63851047 Msa0863810:three_prime_UTR 45.0%
ATTAAAGGCCTTTGCCAGGA+AGG + chr5_4:63851032-63851051 Msa0863810:three_prime_UTR 45.0%
TTGCAAAGTTGGAGACACTG+AGG + chr5_4:63851148-63851167 Msa0863810:three_prime_UTR 45.0%
TGCAAAGTTGGAGACACTGA+GGG + chr5_4:63851149-63851168 Msa0863810:three_prime_UTR 45.0%
GGTTGCTAAAGATGCTGAGT+TGG + chr5_4:63851288-63851307 Msa0863810:three_prime_UTR 45.0%
!!! TAAATTACTTTTTTTTTTTA+GGG - chr5_4:63848904-63848923 None:intergenic 5.0%
!!! TTAAATTACTTTTTTTTTTT+AGG - chr5_4:63848905-63848924 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTTTTG+GGG + chr5_4:63851572-63851591 Msa0863810:three_prime_UTR 5.0%
! TGATGTGAGAGCGCATCGAA+TGG - chr5_4:63848087-63848106 None:intergenic 50.0%
!! TCCGTTTTTGCCGCAGTTCA+AGG + chr5_4:63848146-63848165 Msa0863810:three_prime_UTR 50.0%
ACGGTCCCTAAGTGTCTGTT+TGG - chr5_4:63848581-63848600 None:intergenic 50.0%
ACAGACACTTAGGGACCGTT+TGG + chr5_4:63848582-63848601 Msa0863810:intron 50.0%
CACTTAGGGACCGTTTGGAT+TGG + chr5_4:63848587-63848606 Msa0863810:intron 50.0%
TGGCGGAGATGAATCACGAA+CGG - chr5_4:63849081-63849100 None:intergenic 50.0%
GTAGAAAATGGCGGTCATGC+AGG - chr5_4:63849107-63849126 None:intergenic 50.0%
AAACAGCTTATGGGCCCGTT+TGG - chr5_4:63849499-63849518 None:intergenic 50.0%
GGTGAGGTTTCGGAAATACG+AGG - chr5_4:63849812-63849831 None:intergenic 50.0%
GAGCCAGTGAAGGAAACGAA+GGG - chr5_4:63850022-63850041 None:intergenic 50.0%
TGAGCCAGTGAAGGAAACGA+AGG - chr5_4:63850023-63850042 None:intergenic 50.0%
!! CACTGGCTCACTTCACATTG+TGG + chr5_4:63850033-63850052 Msa0863810:three_prime_UTR 50.0%
GAGGGTTATGCTAGGTTGCT+TGG + chr5_4:63850177-63850196 Msa0863810:three_prime_UTR 50.0%
GGGTTATGCTAGGTTGCTTG+GGG + chr5_4:63850179-63850198 Msa0863810:three_prime_UTR 50.0%
GCTAGGTTGCTTGGGGAAAA+TGG + chr5_4:63850186-63850205 Msa0863810:three_prime_UTR 50.0%
GTTGCTTGGGGAAAATGGGA+TGG + chr5_4:63850191-63850210 Msa0863810:three_prime_UTR 50.0%
TGGGGAAAATGGGATGGTTG+AGG + chr5_4:63850197-63850216 Msa0863810:three_prime_UTR 50.0%
! TGATATGGGGTTGCTTGCTG+AGG + chr5_4:63850731-63850750 Msa0863810:three_prime_UTR 50.0%
AGCTCCTCCAAACACAAAGG+AGG - chr5_4:63851488-63851507 None:intergenic 50.0%
! TCGAATGGAAGAGAGTGCGC+AGG - chr5_4:63848072-63848091 None:intergenic 55.0%
TCTCTGTGCCCATGCTTCCA+TGG + chr5_4:63848234-63848253 Msa0863810:intron 55.0%
TGTGCCCATGCTTCCATGGT+TGG + chr5_4:63848238-63848257 Msa0863810:intron 55.0%
GGAGATGAATCACGAACGGG+CGG - chr5_4:63849077-63849096 None:intergenic 55.0%
GGCGGAGATGAATCACGAAC+GGG - chr5_4:63849080-63849099 None:intergenic 55.0%
! CGTGGCACCGAAGCTTCAAT+AGG - chr5_4:63849703-63849722 None:intergenic 55.0%
! TCGGATTGGAACAGCGTACG+TGG - chr5_4:63849721-63849740 None:intergenic 55.0%
TTGCTGAGGCCAGGAAGTTG+TGG + chr5_4:63850745-63850764 Msa0863810:three_prime_UTR 55.0%
CCTTAACTAGCTCGCCTTCC+TGG - chr5_4:63851049-63851068 None:intergenic 55.0%
CCAGGAAGGCGAGCTAGTTA+AGG + chr5_4:63851046-63851065 Msa0863810:three_prime_UTR 55.0%
GCGAGCTCCTCCAAACACAA+AGG - chr5_4:63851491-63851510 None:intergenic 55.0%
AGTGCGCAGGAGCTTGGCAA+TGG - chr5_4:63848059-63848078 None:intergenic 60.0%
! GAAGAGAGTGCGCAGGAGCT+TGG - chr5_4:63848065-63848084 None:intergenic 60.0%
GAATCACGAACGGGCGGAGA+GGG - chr5_4:63849071-63849090 None:intergenic 60.0%
TGAATCACGAACGGGCGGAG+AGG - chr5_4:63849072-63849091 None:intergenic 60.0%
AATGGCGGTCATGCAGGGAG+TGG - chr5_4:63849101-63849120 None:intergenic 60.0%
CTAGCTCGCCTTCCTGGCAA+AGG - chr5_4:63851043-63851062 None:intergenic 60.0%
GCGCAGGAGCTTGGCAATGG+CGG - chr5_4:63848056-63848075 None:intergenic 65.0%
! TGGGGTTGCTTGCTGAGGCC+AGG + chr5_4:63850736-63850755 Msa0863810:three_prime_UTR 65.0%
GGCGGTCATGCAGGGAGTGG+CGG - chr5_4:63849098-63849117 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 63848043 63851680 63848043 ID=Msa0863810;Name=Msa0863810
chr5_4 mRNA 63848043 63851680 63848043 ID=Msa0863810-mRNA-1;Parent=Msa0863810;Name=Msa0863810-mRNA-1;_AED=0.02;_eAED=0.02;_QI=48|0|1|1|0|0|2|2015|17
chr5_4 exon 63848043 63848167 63848043 ID=Msa0863810-mRNA-1:exon:20059;Parent=Msa0863810-mRNA-1
chr5_4 exon 63849689 63851680 63849689 ID=Msa0863810-mRNA-1:exon:20060;Parent=Msa0863810-mRNA-1
chr5_4 five_prime_UTR 63848043 63848090 63848043 ID=Msa0863810-mRNA-1:five_prime_utr;Parent=Msa0863810-mRNA-1
chr5_4 CDS 63848091 63848144 63848091 ID=Msa0863810-mRNA-1:cds;Parent=Msa0863810-mRNA-1
chr5_4 three_prime_UTR 63848145 63848167 63848145 ID=Msa0863810-mRNA-1:three_prime_utr;Parent=Msa0863810-mRNA-1
chr5_4 three_prime_UTR 63849689 63851680 63849689 ID=Msa0863810-mRNA-1:three_prime_utr;Parent=Msa0863810-mRNA-1
Gene Sequence

>Msa0863810

ATGCGCTCTCACATCATCTCTATCAGTTCCTCAATTTCTCTCCTCTTTCGCTGA
Protein sequence

>Msa0863810

MRSHIISISSSISLLFR*