AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0789910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0789910 MtrunA17_Chr1g0195811 92.056 214 17 0 1 214 1 214 6.93e-149 412
Msa0789910 MtrunA17_Chr1g0195821 86.449 214 29 0 1 214 1 214 4.93e-139 387
Msa0789910 MtrunA17_Chr3g0109891 45.146 206 112 1 4 208 3 208 3.36e-59 185
Msa0789910 MtrunA17_Chr3g0095781 43.541 209 116 2 4 211 3 210 1.05e-57 181
Msa0789910 MtrunA17_Chr1g0195831 39.234 209 126 1 3 210 2 210 4.84e-56 177
Msa0789910 MtrunA17_Chr5g0442751 43.541 209 113 4 4 209 3 209 2.47e-55 175
Msa0789910 MtrunA17_Chr1g0157211 41.667 216 120 3 4 213 5 220 6.76e-55 174
Msa0789910 MtrunA17_Chr5g0442741 41.429 210 120 3 4 211 3 211 4.44e-54 172
Msa0789910 MtrunA17_Chr3g0095821 44.512 164 90 1 49 211 1 164 1.11e-46 152
Msa0789910 MtrunA17_Chr1g0198101 85.294 68 10 0 147 214 1 68 1.79e-38 127
Msa0789910 MtrunA17_Chr3g0125261 22.594 239 153 5 4 210 1 239 6.11e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0789910 AT1G02920.1 62.679 209 78 0 1 209 1 209 1.58e-88 259
Msa0789910 AT1G02930.2 61.244 209 80 1 1 209 1 208 2.00e-87 257
Msa0789910 AT1G02930.1 61.244 209 80 1 1 209 1 208 2.00e-87 257
Msa0789910 AT2G47730.2 57.561 205 86 1 6 209 54 258 1.66e-85 254
Msa0789910 AT2G47730.1 57.561 205 86 1 6 209 54 258 1.66e-85 254
Msa0789910 AT4G02520.1 58.768 211 84 1 1 208 1 211 2.45e-84 249
Msa0789910 AT2G02930.1 58.491 212 85 1 1 209 1 212 1.31e-82 245
Msa0789910 AT1G02950.2 53.883 206 94 1 1 205 24 229 1.83e-77 233
Msa0789910 AT1G02950.3 54.455 202 91 1 5 205 26 227 7.17e-77 231
Msa0789910 AT1G02950.1 54.455 202 91 1 5 205 26 227 7.17e-77 231
Msa0789910 AT1G02950.5 54.455 202 91 1 5 205 38 239 9.54e-77 231
Msa0789910 AT1G02940.4 46.602 206 109 1 5 209 40 245 9.50e-66 203
Msa0789910 AT1G02940.1 46.602 206 109 1 5 209 40 245 9.50e-66 203
Msa0789910 AT1G02940.3 46.602 206 109 1 5 209 53 258 1.12e-65 204
Msa0789910 AT1G02940.2 46.602 206 109 1 5 209 65 270 1.25e-65 204
Msa0789910 AT3G62760.1 47.343 207 108 1 3 208 2 208 8.55e-63 195
Msa0789910 AT1G49860.1 47.179 195 99 2 21 211 22 216 3.28e-57 182
Msa0789910 AT3G03190.1 44.019 209 116 1 4 211 3 211 1.82e-56 178
Msa0789910 AT5G17220.1 38.756 209 127 1 4 211 3 211 2.22e-51 166
Msa0789910 AT2G30860.1 39.904 208 123 2 4 210 3 209 2.62e-49 160
Msa0789910 AT2G30870.1 37.931 203 125 1 9 210 7 209 1.20e-45 151
Msa0789910 AT1G02950.4 50.000 142 70 1 65 205 60 201 1.51e-43 145
Msa0789910 AT2G30860.2 37.500 152 94 1 4 155 3 153 6.04e-31 112
Msa0789910 AT5G41210.1 25.980 204 145 2 1 198 1 204 1.48e-11 62.4
Msa0789910 AT5G41240.1 29.208 202 131 5 4 196 3 201 3.91e-11 62.4

Find 64 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTCACTTCGATTATTTA+TGG 0.098554 5_3:-549925 None:intergenic
CCCCATTATTGCTATCTTCT+TGG 0.169803 5_3:-549473 None:intergenic
TTGGGGATAAAGAGTTTATT+TGG 0.186179 5_3:+549556 Msa0789910:CDS
TTTCAAGTGTACTGTAAAAT+AGG 0.210728 5_3:+550129 Msa0789910:three_prime_UTR
ATCAAGAATGCTATCCAATT+TGG 0.237155 5_3:-549617 None:intergenic
AGAGTCACCTCCCAAGTATT+TGG 0.266829 5_3:-549665 None:intergenic
CTCCAAGAAGATAGCAATAA+TGG 0.269830 5_3:+549471 Msa0789910:CDS
TCAAAACTTGTTCGGGAATT+GGG 0.278093 5_3:+549538 Msa0789910:CDS
ATCAAAACTTGTTCGGGAAT+TGG 0.285433 5_3:+549537 Msa0789910:CDS
GATGGAGATTTAAAGCTCTT+TGG 0.296201 5_3:+549131 Msa0789910:CDS
TGAAGAGAATGAAGCCAAAT+TGG 0.299438 5_3:+549603 Msa0789910:CDS
TCGTGATTCAAACAATTTCT+TGG 0.300130 5_3:-549746 None:intergenic
CGAATCAAAGACATTGGTTA+AGG 0.340841 5_3:+550007 Msa0789910:three_prime_UTR
GAAGAATGGAGAACACAAAA+AGG 0.349354 5_3:+548927 Msa0789910:CDS
GGAGTCATTGCTTGTTAACT+TGG 0.352335 5_3:-549452 None:intergenic
CGCATGGTGGCCGTTGAGTA+GGG 0.361457 5_3:-548847 None:intergenic
AACAATGAGTATGCTGATAA+GGG 0.368137 5_3:+549427 Msa0789910:CDS
TTCTTTATGTTGCAGCCATT+TGG 0.379956 5_3:+549089 Msa0789910:intron
TAACAATGAGTATGCTGATA+AGG 0.387390 5_3:+549426 Msa0789910:CDS
AGTAGCATCAAAACTTGTTC+GGG 0.393847 5_3:+549531 Msa0789910:CDS
AAGTAGCATCAAAACTTGTT+CGG 0.398448 5_3:+549530 Msa0789910:CDS
GAGTTTATTTGGAATTCCTT+TGG 0.399412 5_3:+549567 Msa0789910:CDS
CCGCATGGTGGCCGTTGAGT+AGG 0.403862 5_3:-548848 None:intergenic
TCCAAGAAGATAGCAATAAT+GGG 0.404293 5_3:+549472 Msa0789910:CDS
GTTAACATTAACTTGAAGAA+TGG 0.418899 5_3:+548913 Msa0789910:CDS
AAAATGTAATATTGATTTGT+TGG 0.428291 5_3:+550052 Msa0789910:three_prime_UTR
AGGTGACTCTTTCACCTTGG+TGG 0.445525 5_3:+549678 Msa0789910:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 5_3:-549119 None:intergenic
ACTGTCTTAATTAGGTATCA+TGG 0.449391 5_3:-549830 None:intergenic
GAATCAAAGACATTGGTTAA+GGG 0.449959 5_3:+550008 Msa0789910:three_prime_UTR
GCTTACATTGAGTGATATGA+AGG 0.454820 5_3:-548953 None:intergenic
CACGATCTCATGTTAGTGCT+TGG 0.456189 5_3:+549764 Msa0789910:CDS
AAAATTGAAATGCAGAATCA+AGG 0.457301 5_3:+549386 Msa0789910:intron
ATTGTCTGAGTCCAAATACT+TGG 0.458455 5_3:+549654 Msa0789910:CDS
AAATGTAATATTGATTTGTT+GGG 0.464191 5_3:+550053 Msa0789910:three_prime_UTR
AAAGCTGGAACTTGACCAAA+TGG 0.480424 5_3:-549104 None:intergenic
ACGATCTCATGTTAGTGCTT+GGG 0.482947 5_3:+549765 Msa0789910:CDS
TTGGTTAAGGGATTTGAGTC+CGG 0.493988 5_3:+550020 Msa0789910:three_prime_UTR
GTGATTGATCTGCTAAATCA+TGG 0.497615 5_3:+548801 Msa0789910:exon
ATGCTACTTGATCGAAGTGT+TGG 0.510802 5_3:-549516 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 5_3:+549675 Msa0789910:CDS
TTCATCAAGTAATGAAAAGT+AGG 0.527654 5_3:-549715 None:intergenic
CTACTCAACGGCCACCATGC+GGG 0.529590 5_3:+548849 Msa0789910:CDS
CAAAACTTGTTCGGGAATTG+GGG 0.535983 5_3:+549539 Msa0789910:CDS
GCAAGGACTTTAGACCAAGC+TGG 0.541225 5_3:-549808 None:intergenic
GAATGAAGATTCTTCACTGA+AGG 0.551224 5_3:-549954 None:intergenic
CTTAATTAGGTATCATGGCA+AGG 0.566951 5_3:-549825 None:intergenic
AAATTGAAATGCAGAATCAA+GGG 0.575285 5_3:+549387 Msa0789910:intron
ATATCACTGCAAAACCAGCT+TGG 0.579216 5_3:+549794 Msa0789910:CDS
GCATGGTGGCCGTTGAGTAG+GGG 0.589594 5_3:-548846 None:intergenic
ATAACTCGAATCAAAGACAT+TGG 0.594893 5_3:+550001 Msa0789910:three_prime_UTR
TTGTCTGAGTCCAAATACTT+GGG 0.601763 5_3:+549655 Msa0789910:CDS
ATCTCATGTTAGTGCTTGGG+TGG 0.606175 5_3:+549768 Msa0789910:CDS
CCAAGAAGATAGCAATAATG+GGG 0.612634 5_3:+549473 Msa0789910:CDS
CTTACATTGAGTGATATGAA+GGG 0.613061 5_3:-548952 None:intergenic
GCACGGAAGCCCCTACTCAA+CGG 0.627204 5_3:+548837 Msa0789910:CDS
ACAATGAGTATGCTGATAAG+GGG 0.629847 5_3:+549428 Msa0789910:CDS
ATGGCATCAATAAAAGTGCA+CGG 0.642318 5_3:+548820 Msa0789910:CDS
CAGAGTTGCAGTAACCCGCA+TGG 0.653094 5_3:-548863 None:intergenic
CCTACTCAACGGCCACCATG+CGG 0.660998 5_3:+548848 Msa0789910:CDS
TCTGAGTCCAAATACTTGGG+AGG 0.681272 5_3:+549658 Msa0789910:CDS
CAAGAAGATAGCAATAATGG+GGG 0.701769 5_3:+549474 Msa0789910:CDS
TAAATGATGCAAATCCACCA+AGG 0.702241 5_3:-549692 None:intergenic
AGTTGCAGTAACCCGCATGG+TGG 0.707787 5_3:-548860 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGATAATTTAACAT+TGG - chr5_3:549281-549300 None:intergenic 15.0%
!!! ATCAATATTACATTTTTGAA+AGG - chr5_3:550048-550067 None:intergenic 15.0%
!!! AAAATGTAATATTGATTTGT+TGG + chr5_3:550052-550071 Msa0789910:three_prime_UTR 15.0%
!!! AAATGTAATATTGATTTGTT+GGG + chr5_3:550053-550072 Msa0789910:three_prime_UTR 15.0%
!!! ATACATTGATAATTTAACAT+TGG - chr5_3:549281-549300 None:intergenic 15.0%
!!! ATCAATATTACATTTTTGAA+AGG - chr5_3:550048-550067 None:intergenic 15.0%
!!! AAAATGTAATATTGATTTGT+TGG + chr5_3:550052-550071 Msa0789910:three_prime_UTR 15.0%
!!! AAATGTAATATTGATTTGTT+GGG + chr5_3:550053-550072 Msa0789910:three_prime_UTR 15.0%
!!! TTGTTTTTCAATACTGTTTT+TGG + chr5_3:549879-549898 Msa0789910:three_prime_UTR 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG - chr5_3:549996-550015 None:intergenic 20.0%
!!! TTGTTTTTCAATACTGTTTT+TGG + chr5_3:549879-549898 Msa0789910:three_prime_UTR 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG - chr5_3:549996-550015 None:intergenic 20.0%
!! GTTAACATTAACTTGAAGAA+TGG + chr5_3:548913-548932 Msa0789910:CDS 25.0%
!! ATCAATTAGTTTGATCAATG+TGG - chr5_3:549066-549085 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG + chr5_3:549386-549405 Msa0789910:intron 25.0%
! AAATTGAAATGCAGAATCAA+GGG + chr5_3:549387-549406 Msa0789910:intron 25.0%
!! TCATTCTCTTCAACAATTTT+TGG - chr5_3:549595-549614 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG - chr5_3:549718-549737 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG + chr5_3:549836-549855 Msa0789910:exon 25.0%
! ATCCAAAAACTGTCTTAATT+AGG - chr5_3:549841-549860 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG - chr5_3:550042-550061 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG - chr5_3:550120-550139 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG + chr5_3:550129-550148 Msa0789910:three_prime_UTR 25.0%
!! GTTAACATTAACTTGAAGAA+TGG + chr5_3:548913-548932 Msa0789910:CDS 25.0%
!! ATCAATTAGTTTGATCAATG+TGG - chr5_3:549066-549085 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG + chr5_3:549386-549405 Msa0789910:intron 25.0%
! AAATTGAAATGCAGAATCAA+GGG + chr5_3:549387-549406 Msa0789910:intron 25.0%
!! TCATTCTCTTCAACAATTTT+TGG - chr5_3:549595-549614 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG - chr5_3:549718-549737 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG + chr5_3:549836-549855 Msa0789910:exon 25.0%
! ATCCAAAAACTGTCTTAATT+AGG - chr5_3:549841-549860 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG - chr5_3:550042-550061 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG - chr5_3:550120-550139 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG + chr5_3:550129-550148 Msa0789910:three_prime_UTR 25.0%
CTTACATTGAGTGATATGAA+GGG - chr5_3:548955-548974 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG - chr5_3:549062-549081 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG - chr5_3:549237-549256 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG + chr5_3:549426-549445 Msa0789910:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG + chr5_3:549427-549446 Msa0789910:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG + chr5_3:549472-549491 Msa0789910:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG + chr5_3:549530-549549 Msa0789910:CDS 30.0%
TTGGGGATAAAGAGTTTATT+TGG + chr5_3:549556-549575 Msa0789910:CDS 30.0%
! GAGTTTATTTGGAATTCCTT+TGG + chr5_3:549567-549586 Msa0789910:CDS 30.0%
!! TCAACAATTTTTGGATCCAA+AGG - chr5_3:549586-549605 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG - chr5_3:549620-549639 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG - chr5_3:549749-549768 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG - chr5_3:549833-549852 None:intergenic 30.0%
ACTCTCACTTCGATTATTTA+TGG - chr5_3:549928-549947 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG - chr5_3:549982-550001 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG + chr5_3:550001-550020 Msa0789910:three_prime_UTR 30.0%
! GAATCAAAGACATTGGTTAA+GGG + chr5_3:550008-550027 Msa0789910:three_prime_UTR 30.0%
CTTACATTGAGTGATATGAA+GGG - chr5_3:548955-548974 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG - chr5_3:549062-549081 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG - chr5_3:549237-549256 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG + chr5_3:549426-549445 Msa0789910:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG + chr5_3:549427-549446 Msa0789910:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG + chr5_3:549472-549491 Msa0789910:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG + chr5_3:549530-549549 Msa0789910:CDS 30.0%
TTGGGGATAAAGAGTTTATT+TGG + chr5_3:549556-549575 Msa0789910:CDS 30.0%
! GAGTTTATTTGGAATTCCTT+TGG + chr5_3:549567-549586 Msa0789910:CDS 30.0%
!! TCAACAATTTTTGGATCCAA+AGG - chr5_3:549586-549605 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG - chr5_3:549620-549639 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG - chr5_3:549749-549768 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG - chr5_3:549833-549852 None:intergenic 30.0%
ACTCTCACTTCGATTATTTA+TGG - chr5_3:549928-549947 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG - chr5_3:549982-550001 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG + chr5_3:550001-550020 Msa0789910:three_prime_UTR 30.0%
! GAATCAAAGACATTGGTTAA+GGG + chr5_3:550008-550027 Msa0789910:three_prime_UTR 30.0%
GTGATTGATCTGCTAAATCA+TGG + chr5_3:548801-548820 Msa0789910:exon 35.0%
! ATGGCATCAATAAAAGTGCA+CGG + chr5_3:548820-548839 Msa0789910:CDS 35.0%
GAAGAATGGAGAACACAAAA+AGG + chr5_3:548927-548946 Msa0789910:CDS 35.0%
GCTTACATTGAGTGATATGA+AGG - chr5_3:548956-548975 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG + chr5_3:549089-549108 Msa0789910:intron 35.0%
AAATCTCCATCTTCAAAAGC+TGG - chr5_3:549122-549141 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+TGG + chr5_3:549131-549150 Msa0789910:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG - chr5_3:549225-549244 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG + chr5_3:549428-549447 Msa0789910:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG + chr5_3:549471-549490 Msa0789910:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG + chr5_3:549473-549492 Msa0789910:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG + chr5_3:549474-549493 Msa0789910:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG + chr5_3:549531-549550 Msa0789910:CDS 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG + chr5_3:549537-549556 Msa0789910:CDS 35.0%
! TCAAAACTTGTTCGGGAATT+GGG + chr5_3:549538-549557 Msa0789910:CDS 35.0%
TGAAGAGAATGAAGCCAAAT+TGG + chr5_3:549603-549622 Msa0789910:CDS 35.0%
ATTGTCTGAGTCCAAATACT+TGG + chr5_3:549654-549673 Msa0789910:CDS 35.0%
TTGTCTGAGTCCAAATACTT+GGG + chr5_3:549655-549674 Msa0789910:CDS 35.0%
TAAATGATGCAAATCCACCA+AGG - chr5_3:549695-549714 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG - chr5_3:549828-549847 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG - chr5_3:549957-549976 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG + chr5_3:550007-550026 Msa0789910:three_prime_UTR 35.0%
GTGATTGATCTGCTAAATCA+TGG + chr5_3:548801-548820 Msa0789910:exon 35.0%
! ATGGCATCAATAAAAGTGCA+CGG + chr5_3:548820-548839 Msa0789910:CDS 35.0%
GAAGAATGGAGAACACAAAA+AGG + chr5_3:548927-548946 Msa0789910:CDS 35.0%
GCTTACATTGAGTGATATGA+AGG - chr5_3:548956-548975 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG + chr5_3:549089-549108 Msa0789910:intron 35.0%
AAATCTCCATCTTCAAAAGC+TGG - chr5_3:549122-549141 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+TGG + chr5_3:549131-549150 Msa0789910:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG - chr5_3:549225-549244 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG + chr5_3:549428-549447 Msa0789910:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG + chr5_3:549471-549490 Msa0789910:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG + chr5_3:549473-549492 Msa0789910:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG + chr5_3:549474-549493 Msa0789910:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG + chr5_3:549531-549550 Msa0789910:CDS 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG + chr5_3:549537-549556 Msa0789910:CDS 35.0%
! TCAAAACTTGTTCGGGAATT+GGG + chr5_3:549538-549557 Msa0789910:CDS 35.0%
TGAAGAGAATGAAGCCAAAT+TGG + chr5_3:549603-549622 Msa0789910:CDS 35.0%
ATTGTCTGAGTCCAAATACT+TGG + chr5_3:549654-549673 Msa0789910:CDS 35.0%
TTGTCTGAGTCCAAATACTT+GGG + chr5_3:549655-549674 Msa0789910:CDS 35.0%
TAAATGATGCAAATCCACCA+AGG - chr5_3:549695-549714 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG - chr5_3:549828-549847 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG - chr5_3:549957-549976 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG + chr5_3:550007-550026 Msa0789910:three_prime_UTR 35.0%
TCAATGTGGTTGGAACAAGA+AGG - chr5_3:549052-549071 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG - chr5_3:549107-549126 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG + chr5_3:549113-549132 Msa0789910:CDS 40.0%
! GGAGTCATTGCTTGTTAACT+TGG - chr5_3:549455-549474 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG - chr5_3:549476-549495 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG + chr5_3:549479-549498 Msa0789910:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG - chr5_3:549519-549538 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG + chr5_3:549539-549558 Msa0789910:CDS 40.0%
ACGATCTCATGTTAGTGCTT+GGG + chr5_3:549765-549784 Msa0789910:CDS 40.0%
ATATCACTGCAAAACCAGCT+TGG + chr5_3:549794-549813 Msa0789910:CDS 40.0%
TTGGTTAAGGGATTTGAGTC+CGG + chr5_3:550020-550039 Msa0789910:three_prime_UTR 40.0%
TCAATGTGGTTGGAACAAGA+AGG - chr5_3:549052-549071 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG - chr5_3:549107-549126 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG + chr5_3:549113-549132 Msa0789910:CDS 40.0%
! GGAGTCATTGCTTGTTAACT+TGG - chr5_3:549455-549474 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG - chr5_3:549476-549495 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG + chr5_3:549479-549498 Msa0789910:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG - chr5_3:549519-549538 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG + chr5_3:549539-549558 Msa0789910:CDS 40.0%
ACGATCTCATGTTAGTGCTT+GGG + chr5_3:549765-549784 Msa0789910:CDS 40.0%
ATATCACTGCAAAACCAGCT+TGG + chr5_3:549794-549813 Msa0789910:CDS 40.0%
TTGGTTAAGGGATTTGAGTC+CGG + chr5_3:550020-550039 Msa0789910:three_prime_UTR 40.0%
CTAATATTTGTGCGAGCGCT+TGG + chr5_3:549334-549353 Msa0789910:intron 45.0%
TCTGAGTCCAAATACTTGGG+AGG + chr5_3:549658-549677 Msa0789910:CDS 45.0%
AGAGTCACCTCCCAAGTATT+TGG - chr5_3:549668-549687 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG + chr5_3:549764-549783 Msa0789910:CDS 45.0%
ATCTCATGTTAGTGCTTGGG+TGG + chr5_3:549768-549787 Msa0789910:CDS 45.0%
CTAATATTTGTGCGAGCGCT+TGG + chr5_3:549334-549353 Msa0789910:intron 45.0%
TCTGAGTCCAAATACTTGGG+AGG + chr5_3:549658-549677 Msa0789910:CDS 45.0%
AGAGTCACCTCCCAAGTATT+TGG - chr5_3:549668-549687 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG + chr5_3:549764-549783 Msa0789910:CDS 45.0%
ATCTCATGTTAGTGCTTGGG+TGG + chr5_3:549768-549787 Msa0789910:CDS 45.0%
! AATGGGGGTATGGTCTGAAG+TGG + chr5_3:549489-549508 Msa0789910:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG + chr5_3:549678-549697 Msa0789910:CDS 50.0%
GCAAGGACTTTAGACCAAGC+TGG - chr5_3:549811-549830 None:intergenic 50.0%
! AATGGGGGTATGGTCTGAAG+TGG + chr5_3:549489-549508 Msa0789910:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG + chr5_3:549678-549697 Msa0789910:CDS 50.0%
GCAAGGACTTTAGACCAAGC+TGG - chr5_3:549811-549830 None:intergenic 50.0%
AGTTGCAGTAACCCGCATGG+TGG - chr5_3:548863-548882 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG - chr5_3:548866-548885 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG + chr5_3:549675-549694 Msa0789910:CDS 55.0%
AGTTGCAGTAACCCGCATGG+TGG - chr5_3:548863-548882 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG - chr5_3:548866-548885 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG + chr5_3:549675-549694 Msa0789910:CDS 55.0%
GCACGGAAGCCCCTACTCAA+CGG + chr5_3:548837-548856 Msa0789910:CDS 60.0%
GCATGGTGGCCGTTGAGTAG+GGG - chr5_3:548849-548868 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG - chr5_3:548850-548869 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG + chr5_3:548848-548867 Msa0789910:CDS 60.0%
CTACTCAACGGCCACCATGC+GGG + chr5_3:548849-548868 Msa0789910:CDS 60.0%
GCACGGAAGCCCCTACTCAA+CGG + chr5_3:548837-548856 Msa0789910:CDS 60.0%
GCATGGTGGCCGTTGAGTAG+GGG - chr5_3:548849-548868 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG - chr5_3:548850-548869 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG + chr5_3:548848-548867 Msa0789910:CDS 60.0%
CTACTCAACGGCCACCATGC+GGG + chr5_3:548849-548868 Msa0789910:CDS 60.0%
CCGCATGGTGGCCGTTGAGT+AGG - chr5_3:548851-548870 None:intergenic 65.0%
CCGCATGGTGGCCGTTGAGT+AGG - chr5_3:548851-548870 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 548763 550211 548763 ID=Msa0789910;Name=Msa0789910
chr5_3 mRNA 548763 550211 548763 ID=Msa0789910-mRNA-1;Parent=Msa0789910;Name=Msa0789910-mRNA-1;_AED=0.22;_eAED=0.22;_QI=57|1|1|1|1|1|3|365|214
chr5_3 exon 548763 548969 548763 ID=Msa0789910-mRNA-1:exon:114;Parent=Msa0789910-mRNA-1
chr5_3 exon 549104 549152 549104 ID=Msa0789910-mRNA-1:exon:115;Parent=Msa0789910-mRNA-1
chr5_3 exon 549401 550211 549401 ID=Msa0789910-mRNA-1:exon:116;Parent=Msa0789910-mRNA-1
chr5_3 five_prime_UTR 548763 548819 548763 ID=Msa0789910-mRNA-1:five_prime_utr;Parent=Msa0789910-mRNA-1
chr5_3 CDS 548820 548969 548820 ID=Msa0789910-mRNA-1:cds;Parent=Msa0789910-mRNA-1
chr5_3 CDS 549104 549152 549104 ID=Msa0789910-mRNA-1:cds;Parent=Msa0789910-mRNA-1
chr5_3 CDS 549401 549846 549401 ID=Msa0789910-mRNA-1:cds;Parent=Msa0789910-mRNA-1
chr5_3 three_prime_UTR 549847 550211 549847 ID=Msa0789910-mRNA-1:three_prime_utr;Parent=Msa0789910-mRNA-1
Gene Sequence

>Msa0789910

ATGGCATCAATAAAAGTGCACGGAAGCCCCTACTCAACGGCCACCATGCGGGTTACTGCAACTCTGTATGAAAAACAGATTGAGTTTGAGTTTGTTAACATTAACTTGAAGAATGGAGAACACAAAAAGGAACCCTTCATATCACTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTTGAATCAAGGGCGATAACACAATATATTAACAATGAGTATGCTGATAAGGGGACCAAGTTAACAAGCAATGACTCCAAGAAGATAGCAATAATGGGGGTATGGTCTGAAGTGGAGAGCCAACACTTCGATCAAGTAGCATCAAAACTTGTTCGGGAATTGGGGATAAAGAGTTTATTTGGAATTCCTTTGGATCCAAAAATTGTTGAAGAGAATGAAGCCAAATTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAGTCCAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTACTTTTCATTACTTGATGAAGTCACAGACCAAGAAATTGTTTGAATCACGATCTCATGTTAGTGCTTGGGTGGCTGATATCACTGCAAAACCAGCTTGGTCTAAAGTCCTTGCCATGATACCTAATTAA
Protein sequence

>Msa0789910

MASIKVHGSPYSTATMRVTATLYEKQIEFEFVNINLKNGEHKKEPFISLNPFGQVPAFEDGDLKLFESRAITQYINNEYADKGTKLTSNDSKKIAIMGVWSEVESQHFDQVASKLVRELGIKSLFGIPLDPKIVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPTFHYLMKSQTKKLFESRSHVSAWVADITAKPAWSKVLAMIPN*