AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0129140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129140 MtrunA17_Chr1g0195811 92.523 214 16 0 1 214 1 214 1.31e-149 414
Msa0129140 MtrunA17_Chr1g0195821 86.916 214 28 0 1 214 1 214 1.22e-139 389
Msa0129140 MtrunA17_Chr3g0109891 45.631 206 111 1 4 208 3 208 9.31e-60 186
Msa0129140 MtrunA17_Chr3g0095781 44.019 209 115 2 4 211 3 210 2.86e-58 182
Msa0129140 MtrunA17_Chr1g0195831 39.713 209 125 1 3 210 2 210 1.23e-56 179
Msa0129140 MtrunA17_Chr5g0442751 44.019 209 112 4 4 209 3 209 6.77e-56 177
Msa0129140 MtrunA17_Chr1g0157211 42.130 216 119 3 4 213 5 220 1.94e-55 176
Msa0129140 MtrunA17_Chr5g0442741 41.905 210 119 3 4 211 3 211 1.02e-54 174
Msa0129140 MtrunA17_Chr3g0095821 45.122 164 89 1 49 211 1 164 3.08e-47 153
Msa0129140 MtrunA17_Chr1g0198101 86.765 68 9 0 147 214 1 68 4.98e-39 129
Msa0129140 MtrunA17_Chr3g0125261 23.013 239 152 5 4 210 1 239 2.29e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129140 AT1G02920.1 63.158 209 77 0 1 209 1 209 5.24e-89 261
Msa0129140 AT1G02930.2 61.722 209 79 1 1 209 1 208 6.62e-88 258
Msa0129140 AT1G02930.1 61.722 209 79 1 1 209 1 208 6.62e-88 258
Msa0129140 AT2G47730.2 58.049 205 85 1 6 209 54 258 3.97e-86 256
Msa0129140 AT2G47730.1 58.049 205 85 1 6 209 54 258 3.97e-86 256
Msa0129140 AT4G02520.1 59.242 211 83 1 1 208 1 211 6.52e-85 251
Msa0129140 AT2G02930.1 58.962 212 84 1 1 209 1 212 3.81e-83 246
Msa0129140 AT1G02950.2 54.369 206 93 1 1 205 24 229 5.69e-78 234
Msa0129140 AT1G02950.3 54.950 202 90 1 5 205 26 227 2.71e-77 232
Msa0129140 AT1G02950.1 54.950 202 90 1 5 205 26 227 2.71e-77 232
Msa0129140 AT1G02950.5 54.950 202 90 1 5 205 38 239 2.97e-77 233
Msa0129140 AT1G02940.4 47.087 206 108 1 5 209 40 245 2.66e-66 205
Msa0129140 AT1G02940.1 47.087 206 108 1 5 209 40 245 2.66e-66 205
Msa0129140 AT1G02940.2 47.087 206 108 1 5 209 65 270 3.67e-66 205
Msa0129140 AT1G02940.3 47.087 206 108 1 5 209 53 258 4.51e-66 205
Msa0129140 AT3G62760.1 47.826 207 107 1 3 208 2 208 2.91e-63 196
Msa0129140 AT1G49860.1 47.692 195 98 2 21 211 22 216 7.93e-58 183
Msa0129140 AT3G03190.1 44.498 209 115 1 4 211 3 211 5.87e-57 180
Msa0129140 AT5G17220.1 39.234 209 126 1 4 211 3 211 6.72e-52 167
Msa0129140 AT2G30860.1 40.385 208 122 2 4 210 3 209 5.92e-50 162
Msa0129140 AT2G30870.1 38.424 203 124 1 9 210 7 209 2.84e-46 152
Msa0129140 AT1G02950.4 50.704 142 69 1 65 205 60 201 4.66e-44 147
Msa0129140 AT2G30860.2 37.500 152 94 1 4 155 3 153 6.04e-31 112
Msa0129140 AT5G41210.1 25.980 204 145 2 1 198 1 204 1.48e-11 62.4
Msa0129140 AT5G41240.1 29.208 202 131 5 4 196 3 201 3.88e-11 62.4

Find 64 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTCACTTCGACTATTTA+TGG 0.131208 1_3:+58953807 None:intergenic
CCCCATTATTGCTATCTTCT+TGG 0.169803 1_3:+58954259 None:intergenic
TTGGGGATAAAGAGTTTATT+TGG 0.186179 1_3:-58954176 Msa0129140:CDS
TTTCAAGTGTACTGTAAAAT+AGG 0.210728 1_3:-58953597 Msa0129140:three_prime_UTR
ATCAAGAATGCTATCCAATT+TGG 0.237155 1_3:+58954115 None:intergenic
AGAGTCACCTCCCAAGTATT+TGG 0.266829 1_3:+58954067 None:intergenic
CTCCAAGAAGATAGCAATAA+TGG 0.269830 1_3:-58954261 Msa0129140:CDS
TCAAAACTTGTTCGGGAATT+GGG 0.278093 1_3:-58954194 Msa0129140:CDS
ATCAAAACTTGTTCGGGAAT+TGG 0.285433 1_3:-58954195 Msa0129140:CDS
GATGGAGATTTAAAGCTCTT+TGG 0.296201 1_3:-58954626 Msa0129140:intron
TGAAGAGAATGAAGCCAAAT+TGG 0.299438 1_3:-58954129 Msa0129140:CDS
TCGTGATTCAAACAATTTCT+TGG 0.300130 1_3:+58953986 None:intergenic
CGAATCAAAGACATTGGTTA+AGG 0.340841 1_3:-58953725 Msa0129140:three_prime_UTR
GAAGAATGGAGAACACAAAA+AGG 0.349354 1_3:-58954852 Msa0129140:CDS
GGAGTCATTGCTTGTTAACT+TGG 0.352335 1_3:+58954280 None:intergenic
CGCATGGTGGCCGTTGAGTA+GGG 0.361457 1_3:+58954932 None:intergenic
AACAATGAGTATGCTGATAA+GGG 0.368137 1_3:-58954305 Msa0129140:CDS
TTCTTTATGTTGCAGCCATT+TGG 0.379956 1_3:-58954668 Msa0129140:intron
AATGTAATATATTGATTTGT+TGG 0.385404 1_3:-58953678 Msa0129140:three_prime_UTR
TAACAATGAGTATGCTGATA+AGG 0.387390 1_3:-58954306 Msa0129140:CDS
AGTAGCATCAAAACTTGTTC+GGG 0.393847 1_3:-58954201 Msa0129140:CDS
AAGTAGCATCAAAACTTGTT+CGG 0.398448 1_3:-58954202 Msa0129140:CDS
GAGTTTATTTGGAATTCCTT+TGG 0.399412 1_3:-58954165 Msa0129140:CDS
CCGCATGGTGGCCGTTGAGT+AGG 0.403862 1_3:+58954931 None:intergenic
TCCAAGAAGATAGCAATAAT+GGG 0.404293 1_3:-58954260 Msa0129140:CDS
GTTAACATTAACTTGAAGAA+TGG 0.418899 1_3:-58954866 Msa0129140:CDS
AGGTGACTCTTTCACCTTGG+TGG 0.445525 1_3:-58954054 Msa0129140:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 1_3:+58954638 None:intergenic
ACTGTCTTAATTAGGTATCA+TGG 0.449391 1_3:+58953902 None:intergenic
GAATCAAAGACATTGGTTAA+GGG 0.449959 1_3:-58953724 Msa0129140:three_prime_UTR
GCTTACATTGAGTGATATGA+AGG 0.454820 1_3:+58954826 None:intergenic
CACGATCTCATGTTAGTGCT+TGG 0.456189 1_3:-58953968 Msa0129140:CDS
AAAATTGAAATGCAGAATCA+AGG 0.457301 1_3:-58954346 Msa0129140:intron
ATTGTCTGAGTCCAAATACT+TGG 0.458455 1_3:-58954078 Msa0129140:CDS
ATGTAATATATTGATTTGTT+GGG 0.460128 1_3:-58953677 Msa0129140:three_prime_UTR
AAAGCTGGAACTTGACCAAA+TGG 0.480424 1_3:+58954653 None:intergenic
ACGATCTCATGTTAGTGCTT+GGG 0.482947 1_3:-58953967 Msa0129140:CDS
TTGGTTAAGGGATTTGAGTC+CGG 0.493988 1_3:-58953712 Msa0129140:three_prime_UTR
GTGATTGATCTGCTAAATCA+TGG 0.497615 1_3:-58954978 Msa0129140:exon
ATGCTACTTGATCGAAGTGT+TGG 0.510802 1_3:+58954216 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 1_3:-58954057 Msa0129140:CDS
TTCATCAAGTAATGAAAAGT+AGG 0.527654 1_3:+58954017 None:intergenic
CTACTCAACGGCCACCATGC+GGG 0.529590 1_3:-58954930 Msa0129140:CDS
CAAAACTTGTTCGGGAATTG+GGG 0.535983 1_3:-58954193 Msa0129140:CDS
GAATGAAGATTCTTCACTGA+AGG 0.551224 1_3:+58953778 None:intergenic
CTTAATTAGGTATCATGGCA+AGG 0.566951 1_3:+58953907 None:intergenic
AAATTGAAATGCAGAATCAA+GGG 0.575285 1_3:-58954345 Msa0129140:intron
GCAAGGACTTTAGACCAAGC+TGG 0.580505 1_3:+58953924 None:intergenic
GCATGGTGGCCGTTGAGTAG+GGG 0.589594 1_3:+58954933 None:intergenic
ATAACTCGAATCAAAGACAT+TGG 0.594893 1_3:-58953731 Msa0129140:three_prime_UTR
ATATCACTGCAAGACCAGCT+TGG 0.601324 1_3:-58953938 Msa0129140:CDS
TTGTCTGAGTCCAAATACTT+GGG 0.601763 1_3:-58954077 Msa0129140:CDS
ATCTCATGTTAGTGCTTGGG+TGG 0.606175 1_3:-58953964 Msa0129140:CDS
CCAAGAAGATAGCAATAATG+GGG 0.612634 1_3:-58954259 Msa0129140:CDS
CTTACATTGAGTGATATGAA+GGG 0.613061 1_3:+58954827 None:intergenic
GCACGGAAGCCCCTACTCAA+CGG 0.627204 1_3:-58954942 Msa0129140:CDS
ACAATGAGTATGCTGATAAG+GGG 0.629847 1_3:-58954304 Msa0129140:CDS
ATGGCATCAATAAAAGTGCA+CGG 0.642318 1_3:-58954959 Msa0129140:CDS
GAGAGTTGCAGTAACCCGCA+TGG 0.650972 1_3:+58954916 None:intergenic
CCTACTCAACGGCCACCATG+CGG 0.660998 1_3:-58954931 Msa0129140:CDS
TCTGAGTCCAAATACTTGGG+AGG 0.681272 1_3:-58954074 Msa0129140:CDS
AGTTGCAGTAACCCGCATGG+TGG 0.691159 1_3:+58954919 None:intergenic
CAAGAAGATAGCAATAATGG+GGG 0.701769 1_3:-58954258 Msa0129140:CDS
TAAATGATGCAAATCCACCA+AGG 0.702241 1_3:+58954040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGATAATTTAACAT+TGG + chr1_3:58954078-58954097 None:intergenic 15.0%
!!! CAATATATTACATTTTTGAA+AGG + chr1_3:58954869-58954888 None:intergenic 15.0%
!!! AATGTAATATATTGATTTGT+TGG - chr1_3:58954875-58954894 Msa0129140:CDS 15.0%
!!! ATGTAATATATTGATTTGTT+GGG - chr1_3:58954876-58954895 Msa0129140:CDS 15.0%
!!! ATACATTGATAATTTAACAT+TGG + chr1_3:58954078-58954097 None:intergenic 15.0%
!!! CAATATATTACATTTTTGAA+AGG + chr1_3:58954869-58954888 None:intergenic 15.0%
!!! AATGTAATATATTGATTTGT+TGG - chr1_3:58954875-58954894 Msa0129140:CDS 15.0%
!!! ATGTAATATATTGATTTGTT+GGG - chr1_3:58954876-58954895 Msa0129140:CDS 15.0%
!!! TTGTTTTTCAATTCTGTTTT+TGG - chr1_3:58954700-58954719 Msa0129140:intron 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG + chr1_3:58954817-58954836 None:intergenic 20.0%
!!! TTGTTTTTCAATTCTGTTTT+TGG - chr1_3:58954700-58954719 Msa0129140:intron 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG + chr1_3:58954817-58954836 None:intergenic 20.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1_3:58953687-58953706 Msa0129140:three_prime_UTR 25.0%
!! ATCAATTAGTTTGATCAATG+TGG + chr1_3:58953862-58953881 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1_3:58954207-58954226 Msa0129140:CDS 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1_3:58954208-58954227 Msa0129140:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1_3:58954416-58954435 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1_3:58954539-58954558 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG - chr1_3:58954657-58954676 Msa0129140:intron 25.0%
! ATCCAAAAACTGTCTTAATT+AGG + chr1_3:58954662-58954681 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG + chr1_3:58954863-58954882 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG + chr1_3:58954947-58954966 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG - chr1_3:58954956-58954975 Msa0129140:CDS 25.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1_3:58953687-58953706 Msa0129140:three_prime_UTR 25.0%
!! ATCAATTAGTTTGATCAATG+TGG + chr1_3:58953862-58953881 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1_3:58954207-58954226 Msa0129140:CDS 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1_3:58954208-58954227 Msa0129140:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1_3:58954416-58954435 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1_3:58954539-58954558 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG - chr1_3:58954657-58954676 Msa0129140:intron 25.0%
! ATCCAAAAACTGTCTTAATT+AGG + chr1_3:58954662-58954681 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG + chr1_3:58954863-58954882 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG + chr1_3:58954947-58954966 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG - chr1_3:58954956-58954975 Msa0129140:CDS 25.0%
CTTACATTGAGTGATATGAA+GGG + chr1_3:58953729-58953748 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG + chr1_3:58953858-58953877 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG + chr1_3:58954033-58954052 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1_3:58954247-58954266 Msa0129140:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG - chr1_3:58954248-58954267 Msa0129140:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1_3:58954293-58954312 Msa0129140:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1_3:58954351-58954370 Msa0129140:intron 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1_3:58954377-58954396 Msa0129140:intron 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1_3:58954388-58954407 Msa0129140:intron 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1_3:58954407-58954426 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG + chr1_3:58954441-58954460 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1_3:58954570-58954589 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG + chr1_3:58954654-58954673 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG + chr1_3:58954803-58954822 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG - chr1_3:58954822-58954841 Msa0129140:intron 30.0%
! GAATCAAAGACATTGGTTAA+GGG - chr1_3:58954829-58954848 Msa0129140:intron 30.0%
CTTACATTGAGTGATATGAA+GGG + chr1_3:58953729-58953748 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG + chr1_3:58953858-58953877 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG + chr1_3:58954033-58954052 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1_3:58954247-58954266 Msa0129140:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG - chr1_3:58954248-58954267 Msa0129140:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1_3:58954293-58954312 Msa0129140:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1_3:58954351-58954370 Msa0129140:intron 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1_3:58954377-58954396 Msa0129140:intron 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1_3:58954388-58954407 Msa0129140:intron 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1_3:58954407-58954426 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG + chr1_3:58954441-58954460 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1_3:58954570-58954589 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG + chr1_3:58954654-58954673 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG + chr1_3:58954803-58954822 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG - chr1_3:58954822-58954841 Msa0129140:intron 30.0%
! GAATCAAAGACATTGGTTAA+GGG - chr1_3:58954829-58954848 Msa0129140:intron 30.0%
GTGATTGATCTGCTAAATCA+TGG - chr1_3:58953575-58953594 Msa0129140:three_prime_UTR 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1_3:58953594-58953613 Msa0129140:three_prime_UTR 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1_3:58953701-58953720 Msa0129140:three_prime_UTR 35.0%
GCTTACATTGAGTGATATGA+AGG + chr1_3:58953730-58953749 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1_3:58953885-58953904 Msa0129140:three_prime_UTR 35.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1_3:58953918-58953937 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+TGG - chr1_3:58953927-58953946 Msa0129140:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG + chr1_3:58954021-58954040 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1_3:58954249-58954268 Msa0129140:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1_3:58954292-58954311 Msa0129140:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1_3:58954294-58954313 Msa0129140:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1_3:58954295-58954314 Msa0129140:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1_3:58954352-58954371 Msa0129140:intron 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1_3:58954358-58954377 Msa0129140:intron 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1_3:58954359-58954378 Msa0129140:intron 35.0%
TGAAGAGAATGAAGCCAAAT+TGG - chr1_3:58954424-58954443 Msa0129140:intron 35.0%
ATTGTCTGAGTCCAAATACT+TGG - chr1_3:58954475-58954494 Msa0129140:intron 35.0%
TTGTCTGAGTCCAAATACTT+GGG - chr1_3:58954476-58954495 Msa0129140:intron 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1_3:58954516-58954535 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG + chr1_3:58954649-58954668 None:intergenic 35.0%
ACTCTCACTTCGACTATTTA+TGG + chr1_3:58954749-58954768 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG + chr1_3:58954778-58954797 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG - chr1_3:58954828-58954847 Msa0129140:intron 35.0%
GTGATTGATCTGCTAAATCA+TGG - chr1_3:58953575-58953594 Msa0129140:three_prime_UTR 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1_3:58953594-58953613 Msa0129140:three_prime_UTR 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1_3:58953701-58953720 Msa0129140:three_prime_UTR 35.0%
GCTTACATTGAGTGATATGA+AGG + chr1_3:58953730-58953749 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1_3:58953885-58953904 Msa0129140:three_prime_UTR 35.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1_3:58953918-58953937 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+TGG - chr1_3:58953927-58953946 Msa0129140:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG + chr1_3:58954021-58954040 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1_3:58954249-58954268 Msa0129140:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1_3:58954292-58954311 Msa0129140:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1_3:58954294-58954313 Msa0129140:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1_3:58954295-58954314 Msa0129140:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1_3:58954352-58954371 Msa0129140:intron 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1_3:58954358-58954377 Msa0129140:intron 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1_3:58954359-58954378 Msa0129140:intron 35.0%
TGAAGAGAATGAAGCCAAAT+TGG - chr1_3:58954424-58954443 Msa0129140:intron 35.0%
ATTGTCTGAGTCCAAATACT+TGG - chr1_3:58954475-58954494 Msa0129140:intron 35.0%
TTGTCTGAGTCCAAATACTT+GGG - chr1_3:58954476-58954495 Msa0129140:intron 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1_3:58954516-58954535 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG + chr1_3:58954649-58954668 None:intergenic 35.0%
ACTCTCACTTCGACTATTTA+TGG + chr1_3:58954749-58954768 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG + chr1_3:58954778-58954797 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG - chr1_3:58954828-58954847 Msa0129140:intron 35.0%
TCAATGTGGTTGGAACAAGA+AGG + chr1_3:58953848-58953867 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1_3:58953903-58953922 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1_3:58953909-58953928 Msa0129140:CDS 40.0%
TGTCATGTGCTACGTAAATC+AGG + chr1_3:58953968-58953987 None:intergenic 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1_3:58954276-58954295 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1_3:58954297-58954316 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1_3:58954300-58954319 Msa0129140:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1_3:58954340-58954359 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1_3:58954360-58954379 Msa0129140:intron 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1_3:58954586-58954605 Msa0129140:intron 40.0%
TTGGTTAAGGGATTTGAGTC+CGG - chr1_3:58954841-58954860 Msa0129140:CDS 40.0%
TCAATGTGGTTGGAACAAGA+AGG + chr1_3:58953848-58953867 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1_3:58953903-58953922 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1_3:58953909-58953928 Msa0129140:CDS 40.0%
TGTCATGTGCTACGTAAATC+AGG + chr1_3:58953968-58953987 None:intergenic 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1_3:58954276-58954295 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1_3:58954297-58954316 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1_3:58954300-58954319 Msa0129140:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1_3:58954340-58954359 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1_3:58954360-58954379 Msa0129140:intron 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1_3:58954586-58954605 Msa0129140:intron 40.0%
TTGGTTAAGGGATTTGAGTC+CGG - chr1_3:58954841-58954860 Msa0129140:CDS 40.0%
CTAATATTTGTGCGAGCGCT+TGG - chr1_3:58954155-58954174 Msa0129140:CDS 45.0%
TCTGAGTCCAAATACTTGGG+AGG - chr1_3:58954479-58954498 Msa0129140:intron 45.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1_3:58954489-58954508 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1_3:58954585-58954604 Msa0129140:intron 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1_3:58954589-58954608 Msa0129140:intron 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1_3:58954615-58954634 Msa0129140:intron 45.0%
CTAATATTTGTGCGAGCGCT+TGG - chr1_3:58954155-58954174 Msa0129140:CDS 45.0%
TCTGAGTCCAAATACTTGGG+AGG - chr1_3:58954479-58954498 Msa0129140:intron 45.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1_3:58954489-58954508 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1_3:58954585-58954604 Msa0129140:intron 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1_3:58954589-58954608 Msa0129140:intron 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1_3:58954615-58954634 Msa0129140:intron 45.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1_3:58954310-58954329 Msa0129140:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1_3:58954499-58954518 Msa0129140:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1_3:58954632-58954651 None:intergenic 50.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1_3:58954310-58954329 Msa0129140:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1_3:58954499-58954518 Msa0129140:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1_3:58954632-58954651 None:intergenic 50.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1_3:58953637-58953656 None:intergenic 55.0%
GAGAGTTGCAGTAACCCGCA+TGG + chr1_3:58953640-58953659 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1_3:58954496-58954515 Msa0129140:intron 55.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1_3:58953637-58953656 None:intergenic 55.0%
GAGAGTTGCAGTAACCCGCA+TGG + chr1_3:58953640-58953659 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1_3:58954496-58954515 Msa0129140:intron 55.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1_3:58953611-58953630 Msa0129140:three_prime_UTR 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1_3:58953623-58953642 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1_3:58953624-58953643 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG - chr1_3:58953622-58953641 Msa0129140:three_prime_UTR 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1_3:58953623-58953642 Msa0129140:three_prime_UTR 60.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1_3:58953611-58953630 Msa0129140:three_prime_UTR 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1_3:58953623-58953642 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1_3:58953624-58953643 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG - chr1_3:58953622-58953641 Msa0129140:three_prime_UTR 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1_3:58953623-58953642 Msa0129140:three_prime_UTR 60.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1_3:58953625-58953644 None:intergenic 65.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1_3:58953625-58953644 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58953537 58955038 58953537 ID=Msa0129140;Name=Msa0129140
chr1_3 mRNA 58953537 58955038 58953537 ID=Msa0129140-mRNA-1;Parent=Msa0129140;Name=Msa0129140-mRNA-1;_AED=0.23;_eAED=0.23;_QI=57|1|1|1|1|1|3|371|214
chr1_3 exon 58953537 58954353 58953537 ID=Msa0129140-mRNA-1:exon:19088;Parent=Msa0129140-mRNA-1
chr1_3 exon 58954627 58954675 58954627 ID=Msa0129140-mRNA-1:exon:19087;Parent=Msa0129140-mRNA-1
chr1_3 exon 58954832 58955038 58954832 ID=Msa0129140-mRNA-1:exon:19086;Parent=Msa0129140-mRNA-1
chr1_3 five_prime_UTR 58954982 58955038 58954982 ID=Msa0129140-mRNA-1:five_prime_utr;Parent=Msa0129140-mRNA-1
chr1_3 CDS 58954832 58954981 58954832 ID=Msa0129140-mRNA-1:cds;Parent=Msa0129140-mRNA-1
chr1_3 CDS 58954627 58954675 58954627 ID=Msa0129140-mRNA-1:cds;Parent=Msa0129140-mRNA-1
chr1_3 CDS 58953908 58954353 58953908 ID=Msa0129140-mRNA-1:cds;Parent=Msa0129140-mRNA-1
chr1_3 three_prime_UTR 58953537 58953907 58953537 ID=Msa0129140-mRNA-1:three_prime_utr;Parent=Msa0129140-mRNA-1
Gene Sequence

>Msa0129140

ATGGCATCAATAAAAGTGCACGGAAGCCCCTACTCAACGGCCACCATGCGGGTTACTGCAACTCTCTATGAAAAACAGATTGAGTTTGAGTTTGTTAACATTAACTTGAAGAATGGAGAACACAAAAAGGAACCCTTCATATCACTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTTGAATCAAGGGCGATAACACAATATATTAACAATGAGTATGCTGATAAGGGGACCAAGTTAACAAGCAATGACTCCAAGAAGATAGCAATAATGGGGGTATGGTCTGAAGTGGAGAGCCAACACTTCGATCAAGTAGCATCAAAACTTGTTCGGGAATTGGGGATAAAGAGTTTATTTGGAATTCCTTTGGATCCAAAAATTGTTGAAGAGAATGAAGCCAAATTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAGTCCAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTACTTTTCATTACTTGATGAAGTCACAGACCAAGAAATTGTTTGAATCACGATCTCATGTTAGTGCTTGGGTGGCTGATATCACTGCAAGACCAGCTTGGTCTAAAGTCCTTGCCATGATACCTAATTAA
Protein sequence

>Msa0129140

MASIKVHGSPYSTATMRVTATLYEKQIEFEFVNINLKNGEHKKEPFISLNPFGQVPAFEDGDLKLFESRAITQYINNEYADKGTKLTSNDSKKIAIMGVWSEVESQHFDQVASKLVRELGIKSLFGIPLDPKIVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPTFHYLMKSQTKKLFESRSHVSAWVADITARPAWSKVLAMIPN*