AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0793670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0793670 MtrunA17_Chr5g0394521 94.964 139 6 1 1 138 1 139 8.28e-94 267
Msa0793670 MtrunA17_Chr5g0394511 66.906 139 38 4 6 137 3 140 6.72e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0793670 AT2G32090.1 54.074 135 46 4 7 130 2 131 2.47e-50 157
Msa0793670 AT2G32090.2 52.212 113 38 4 29 130 2 109 7.12e-38 125
Msa0793670 AT2G28420.1 29.457 129 80 3 7 130 19 141 6.64e-12 60.5

Find 74 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTTATCCAACGAAATT+AGG 0.180713 5_3:-5576071 Msa0793670:five_prime_UTR
TCCTCCTAGTTACTTTAATT+TGG 0.200302 5_3:-5575177 Msa0793670:exon
ACTTACCTCTCGGTAGAATT+TGG 0.203336 5_3:+5575908 None:intergenic
AGAAAAGATTTCTATTGATT+TGG 0.249533 5_3:+5576016 None:intergenic
TATTGATTTGGAAACAATAA+TGG 0.294612 5_3:+5576028 None:intergenic
AAAGAAATTACCAAGGAATA+TGG 0.297272 5_3:-5574787 Msa0793670:three_prime_UTR
ACACAAACTACAGTAAAATT+AGG 0.312173 5_3:+5574979 None:intergenic
TTGGACAGTTCAAGATTATA+TGG 0.312649 5_3:-5575684 Msa0793670:CDS
TTGTGTGTTGCCTGCGGTTT+GGG 0.319401 5_3:-5574962 Msa0793670:three_prime_UTR
CACTACTTGTGATTTCTTGT+CGG 0.326553 5_3:-5575060 Msa0793670:three_prime_UTR
TGATTGAGTTATCAGATGTT+TGG 0.331923 5_3:-5575733 Msa0793670:intron
GATTGAGTTATCAGATGTTT+GGG 0.363031 5_3:-5575732 Msa0793670:intron
TGTTTAGAAGGGTCGACTAT+AGG 0.382836 5_3:-5575032 Msa0793670:three_prime_UTR
AGTCAAAGTTAATTAAGATA+TGG 0.385112 5_3:+5574714 None:intergenic
ATCTTGAACTGTCCAAAGTC+AGG 0.387838 5_3:+5575691 None:intergenic
CTGGTAGCATTGTTTCCATT+AGG 0.390280 5_3:-5574900 Msa0793670:three_prime_UTR
TTATCAGATGTTTGGGTTTG+AGG 0.401608 5_3:-5575725 Msa0793670:intron
ATATGAGAGGGGTCTTTAAC+AGG 0.405224 5_3:+5575586 None:intergenic
TTTGTGTGTTGCCTGCGGTT+TGG 0.409406 5_3:-5574963 Msa0793670:three_prime_UTR
CGGTGGACTCTCGAGCTATA+TGG 0.414336 5_3:+5575946 None:intergenic
TCTAGAAAAGATAGAAGAAA+TGG 0.416833 5_3:-5575994 Msa0793670:exon
ACTACTTGTGATTTCTTGTC+GGG 0.422987 5_3:-5575059 Msa0793670:three_prime_UTR
AACGTGAACAAACCTCCTAA+TGG 0.431030 5_3:+5574885 None:intergenic
TGTTGTAAGTCTGCTTGCAC+AGG 0.453222 5_3:+5575009 None:intergenic
GAAGTTGAGACCCCTGACTT+TGG 0.454246 5_3:-5575703 Msa0793670:CDS
TTTCAAATCATCACATGGGT+AGG 0.475491 5_3:-5576090 Msa0793670:five_prime_UTR
GTTGTGAAAGGAATGAAGAT+TGG 0.479221 5_3:+5575531 None:intergenic
CATTGTTTCAAATCATCACA+TGG 0.485619 5_3:-5576095 Msa0793670:five_prime_UTR
ATCTAGCACCCTAATTTCGT+TGG 0.488283 5_3:+5576062 None:intergenic
AAGACTAGGTTGATGATAGT+TGG 0.508283 5_3:+5575101 None:intergenic
AGATGGAGAAGCAGAGATGA+TGG 0.508377 5_3:+5575554 None:intergenic
GCAAGCAGACTTACAACAAA+AGG 0.511066 5_3:-5575004 Msa0793670:three_prime_UTR
TACCAGTTGACAAAATGAAA+GGG 0.515688 5_3:+5574917 None:intergenic
TGTATATGCAGGTAATGGCT+TGG 0.518965 5_3:-5575223 Msa0793670:intron
GACGAGGTATCCATATTCCT+TGG 0.519059 5_3:+5574777 None:intergenic
TTCAAATCATCACATGGGTA+GGG 0.520517 5_3:-5576089 Msa0793670:five_prime_UTR
CGTTGCTACGTTGTATGAGA+TGG 0.521284 5_3:+5575632 None:intergenic
AGAGGGGTCTTTAACAGGAA+TGG 0.522033 5_3:+5575591 None:intergenic
TTCCTTTCACAACACTCTCA+AGG 0.527218 5_3:-5575521 Msa0793670:intron
AGGGTTATCCAACGAAATTA+GGG 0.528572 5_3:-5576070 Msa0793670:five_prime_UTR
GTAGCATTGTTTCCATTAGG+AGG 0.531970 5_3:-5574897 Msa0793670:three_prime_UTR
GAAGAAATGGCCGCAGCAGA+AGG 0.534245 5_3:-5575981 Msa0793670:exon
CTACCAGTTGACAAAATGAA+AGG 0.534782 5_3:+5574916 None:intergenic
TCTTGAACTGTCCAAAGTCA+GGG 0.541425 5_3:+5575692 None:intergenic
TGGATAAGGAAGGATGAAGA+AGG 0.546868 5_3:+5575652 None:intergenic
AATAAAGCCACCCAAACCGC+AGG 0.546895 5_3:+5574952 None:intergenic
GTCTTAATTAATTACCATCA+GGG 0.550683 5_3:+5575341 None:intergenic
TACGTTGTATGAGATGGATA+AGG 0.553336 5_3:+5575638 None:intergenic
TGATTTGAAACAATGAAAGA+TGG 0.554494 5_3:+5576103 None:intergenic
TTAACAGGAATGGATGATGA+TGG 0.555627 5_3:+5575601 None:intergenic
CAGTATCATTAATCAAGACT+AGG 0.565450 5_3:+5575087 None:intergenic
AACCTTGAGAGTGTTGTGAA+AGG 0.570775 5_3:+5575519 None:intergenic
AAAGGAATGAAGATTGGAGA+TGG 0.571006 5_3:+5575537 None:intergenic
ATATGCAGGTAATGGCTTGG+AGG 0.578557 5_3:-5575220 Msa0793670:intron
TGGCCTAAGCGGATATGAGA+GGG 0.586943 5_3:+5575574 None:intergenic
CATACAACGTAGCAACGAGC+TGG 0.597863 5_3:-5575626 Msa0793670:CDS
TTGTATGAGATGGATAAGGA+AGG 0.611019 5_3:+5575642 None:intergenic
TGTGTTGCCTGCGGTTTGGG+TGG 0.612077 5_3:-5574959 Msa0793670:three_prime_UTR
AGCAGAGATGATGGCCTAAG+CGG 0.614263 5_3:+5575563 None:intergenic
ATGGCCTAAGCGGATATGAG+AGG 0.629456 5_3:+5575573 None:intergenic
ATTGTTTCAAATCATCACAT+GGG 0.632503 5_3:-5576094 Msa0793670:five_prime_UTR
GACCCCTCTCATATCCGCTT+AGG 0.637582 5_3:-5575577 Msa0793670:CDS
ACCAGTTGACAAAATGAAAG+GGG 0.643412 5_3:+5574918 None:intergenic
TAAGGAAGGATGAAGAAGGA+AGG 0.647624 5_3:+5575656 None:intergenic
ACCAAATTAAAGTAACTAGG+AGG 0.648939 5_3:+5575176 None:intergenic
TGTAGTTTGTGTGTTGCCTG+CGG 0.657320 5_3:-5574968 Msa0793670:three_prime_UTR
GACACCAAATTAAAGTAACT+AGG 0.660293 5_3:+5575173 None:intergenic
CTTGAACTGTCCAAAGTCAG+GGG 0.685610 5_3:+5575693 None:intergenic
TGATTTGGAAACAATAATGG+TGG 0.685697 5_3:+5576031 None:intergenic
AAAGCTAAAAGAAATTACCA+AGG 0.689700 5_3:-5574794 Msa0793670:three_prime_UTR
CAGACTCACTCCTTCTGCTG+CGG 0.692086 5_3:+5575971 None:intergenic
GGCCTAAGCGGATATGAGAG+GGG 0.694889 5_3:+5575575 None:intergenic
GGACATCAAAGTAGATGACG+AGG 0.710844 5_3:+5574761 None:intergenic
ACTCTCCAAATTCTACCGAG+AGG 0.710906 5_3:-5575913 Msa0793670:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAATTATTATCTGTG+TGG - chr5_3:5575018-5575037 Msa0793670:three_prime_UTR 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG + chr5_3:5576086-5576105 None:intergenic 15.0%
!!! TTTTTAAATTATTATCTGTG+TGG - chr5_3:5575018-5575037 Msa0793670:three_prime_UTR 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG + chr5_3:5576086-5576105 None:intergenic 15.0%
!!! TATTGATTTGGAAACAATAA+TGG + chr5_3:5574798-5574817 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG + chr5_3:5574810-5574829 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG + chr5_3:5576112-5576131 None:intergenic 20.0%
!!! TATTGATTTGGAAACAATAA+TGG + chr5_3:5574798-5574817 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG + chr5_3:5574810-5574829 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG + chr5_3:5576112-5576131 None:intergenic 20.0%
!! TGATTTGAAACAATGAAAGA+TGG + chr5_3:5574723-5574742 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG - chr5_3:5574729-5574748 Msa0793670:three_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG - chr5_3:5574829-5574848 Msa0793670:three_prime_UTR 25.0%
! TTAAATACTAACTTACCTCT+CGG + chr5_3:5574928-5574947 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG - chr5_3:5575398-5575417 Msa0793670:CDS 25.0%
!!! CATTTTTTTATACAGGACAA+AGG - chr5_3:5575405-5575424 Msa0793670:CDS 25.0%
! GTCTTAATTAATTACCATCA+GGG + chr5_3:5575485-5575504 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG + chr5_3:5575486-5575505 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG - chr5_3:5575546-5575565 Msa0793670:CDS 25.0%
! GTAGTATTAATTGTATATGC+AGG - chr5_3:5575589-5575608 Msa0793670:CDS 25.0%
! TTAATTGTATATGCAGGTAA+TGG - chr5_3:5575595-5575614 Msa0793670:CDS 25.0%
! ACACAAACTACAGTAAAATT+AGG + chr5_3:5575847-5575866 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG + chr5_3:5576030-5576049 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG - chr5_3:5576029-5576048 Msa0793670:five_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG - chr5_3:5576036-5576055 Msa0793670:five_prime_UTR 25.0%
!! TGATTTGAAACAATGAAAGA+TGG + chr5_3:5574723-5574742 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG - chr5_3:5574729-5574748 Msa0793670:three_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG - chr5_3:5574829-5574848 Msa0793670:three_prime_UTR 25.0%
! TTAAATACTAACTTACCTCT+CGG + chr5_3:5574928-5574947 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG - chr5_3:5575398-5575417 Msa0793670:CDS 25.0%
!!! CATTTTTTTATACAGGACAA+AGG - chr5_3:5575405-5575424 Msa0793670:CDS 25.0%
! GTCTTAATTAATTACCATCA+GGG + chr5_3:5575485-5575504 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG + chr5_3:5575486-5575505 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG - chr5_3:5575546-5575565 Msa0793670:CDS 25.0%
! GTAGTATTAATTGTATATGC+AGG - chr5_3:5575589-5575608 Msa0793670:CDS 25.0%
! TTAATTGTATATGCAGGTAA+TGG - chr5_3:5575595-5575614 Msa0793670:CDS 25.0%
! ACACAAACTACAGTAAAATT+AGG + chr5_3:5575847-5575866 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG + chr5_3:5576030-5576049 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG - chr5_3:5576029-5576048 Msa0793670:five_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG - chr5_3:5576036-5576055 Msa0793670:five_prime_UTR 25.0%
CATTGTTTCAAATCATCACA+TGG - chr5_3:5574728-5574747 Msa0793670:three_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG + chr5_3:5574795-5574814 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG + chr5_3:5575069-5575088 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG - chr5_3:5575090-5575109 Msa0793670:three_prime_UTR 30.0%
GATTGAGTTATCAGATGTTT+GGG - chr5_3:5575091-5575110 Msa0793670:three_prime_UTR 30.0%
TTGGACAGTTCAAGATTATA+TGG - chr5_3:5575139-5575158 Msa0793670:three_prime_UTR 30.0%
AAACTCAAATCTAACAACAG+TGG + chr5_3:5575347-5575366 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG - chr5_3:5575646-5575665 Msa0793670:CDS 30.0%
ACCAAATTAAAGTAACTAGG+AGG + chr5_3:5575650-5575669 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG + chr5_3:5575653-5575672 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG + chr5_3:5575739-5575758 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG - chr5_3:5575882-5575901 Msa0793670:intron 30.0%
TACCAGTTGACAAAATGAAA+GGG + chr5_3:5575909-5575928 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG + chr5_3:5576022-5576041 None:intergenic 30.0%
CATTGTTTCAAATCATCACA+TGG - chr5_3:5574728-5574747 Msa0793670:three_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG + chr5_3:5574795-5574814 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG + chr5_3:5575069-5575088 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG - chr5_3:5575090-5575109 Msa0793670:three_prime_UTR 30.0%
GATTGAGTTATCAGATGTTT+GGG - chr5_3:5575091-5575110 Msa0793670:three_prime_UTR 30.0%
TTGGACAGTTCAAGATTATA+TGG - chr5_3:5575139-5575158 Msa0793670:three_prime_UTR 30.0%
AAACTCAAATCTAACAACAG+TGG + chr5_3:5575347-5575366 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG - chr5_3:5575646-5575665 Msa0793670:CDS 30.0%
ACCAAATTAAAGTAACTAGG+AGG + chr5_3:5575650-5575669 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG + chr5_3:5575653-5575672 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG + chr5_3:5575739-5575758 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG - chr5_3:5575882-5575901 Msa0793670:intron 30.0%
TACCAGTTGACAAAATGAAA+GGG + chr5_3:5575909-5575928 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG + chr5_3:5576022-5576041 None:intergenic 30.0%
TTTCAAATCATCACATGGGT+AGG - chr5_3:5574733-5574752 Msa0793670:three_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG - chr5_3:5574734-5574753 Msa0793670:three_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG - chr5_3:5574752-5574771 Msa0793670:three_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG - chr5_3:5574753-5574772 Msa0793670:three_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG + chr5_3:5574900-5574919 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG - chr5_3:5575098-5575117 Msa0793670:three_prime_UTR 35.0%
TTGTATGAGATGGATAAGGA+AGG + chr5_3:5575184-5575203 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG + chr5_3:5575188-5575207 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG + chr5_3:5575225-5575244 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG + chr5_3:5575289-5575308 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG + chr5_3:5575295-5575314 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG - chr5_3:5575432-5575451 Msa0793670:intron 35.0%
TACGTGATCATCTCATCTTA+GGG + chr5_3:5575572-5575591 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG + chr5_3:5575725-5575744 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG - chr5_3:5575763-5575782 Msa0793670:intron 35.0%
ACTACTTGTGATTTCTTGTC+GGG - chr5_3:5575764-5575783 Msa0793670:intron 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG - chr5_3:5575779-5575798 Msa0793670:intron 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG - chr5_3:5575780-5575799 Msa0793670:intron 35.0%
ACCAGTTGACAAAATGAAAG+GGG + chr5_3:5575908-5575927 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG + chr5_3:5575910-5575929 None:intergenic 35.0%
TTTCAAATCATCACATGGGT+AGG - chr5_3:5574733-5574752 Msa0793670:three_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG - chr5_3:5574734-5574753 Msa0793670:three_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG - chr5_3:5574752-5574771 Msa0793670:three_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG - chr5_3:5574753-5574772 Msa0793670:three_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG + chr5_3:5574900-5574919 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG - chr5_3:5575098-5575117 Msa0793670:three_prime_UTR 35.0%
TTGTATGAGATGGATAAGGA+AGG + chr5_3:5575184-5575203 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG + chr5_3:5575188-5575207 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG + chr5_3:5575225-5575244 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG + chr5_3:5575289-5575308 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG + chr5_3:5575295-5575314 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG - chr5_3:5575432-5575451 Msa0793670:intron 35.0%
TACGTGATCATCTCATCTTA+GGG + chr5_3:5575572-5575591 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG + chr5_3:5575725-5575744 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG - chr5_3:5575763-5575782 Msa0793670:intron 35.0%
ACTACTTGTGATTTCTTGTC+GGG - chr5_3:5575764-5575783 Msa0793670:intron 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG - chr5_3:5575779-5575798 Msa0793670:intron 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG - chr5_3:5575780-5575799 Msa0793670:intron 35.0%
ACCAGTTGACAAAATGAAAG+GGG + chr5_3:5575908-5575927 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG + chr5_3:5575910-5575929 None:intergenic 35.0%
ATCTAGCACCCTAATTTCGT+TGG + chr5_3:5574764-5574783 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG + chr5_3:5574918-5574937 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG + chr5_3:5574951-5574970 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG + chr5_3:5575049-5575068 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG + chr5_3:5575134-5575153 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG + chr5_3:5575135-5575154 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG + chr5_3:5575170-5575189 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG + chr5_3:5575174-5575193 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG + chr5_3:5575240-5575259 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG - chr5_3:5575302-5575321 Msa0793670:intron 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG + chr5_3:5575307-5575326 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG - chr5_3:5575468-5575487 Msa0793670:intron 40.0%
CATCTTAGGGCCAATAAACA+AGG + chr5_3:5575559-5575578 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG + chr5_3:5575573-5575592 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG - chr5_3:5575600-5575619 Msa0793670:CDS 40.0%
TGTTTAGAAGGGTCGACTAT+AGG - chr5_3:5575791-5575810 Msa0793670:intron 40.0%
GCAAGCAGACTTACAACAAA+AGG - chr5_3:5575819-5575838 Msa0793670:intron 40.0%
CTGGTAGCATTGTTTCCATT+AGG - chr5_3:5575923-5575942 Msa0793670:CDS 40.0%
GTAGCATTGTTTCCATTAGG+AGG - chr5_3:5575926-5575945 Msa0793670:CDS 40.0%
AACGTGAACAAACCTCCTAA+TGG + chr5_3:5575941-5575960 None:intergenic 40.0%
ATCTAGCACCCTAATTTCGT+TGG + chr5_3:5574764-5574783 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG + chr5_3:5574918-5574937 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG + chr5_3:5574951-5574970 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG + chr5_3:5575049-5575068 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG + chr5_3:5575134-5575153 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG + chr5_3:5575135-5575154 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG + chr5_3:5575170-5575189 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG + chr5_3:5575174-5575193 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG + chr5_3:5575240-5575259 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG - chr5_3:5575302-5575321 Msa0793670:intron 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG + chr5_3:5575307-5575326 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG - chr5_3:5575468-5575487 Msa0793670:intron 40.0%
CATCTTAGGGCCAATAAACA+AGG + chr5_3:5575559-5575578 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG + chr5_3:5575573-5575592 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG - chr5_3:5575600-5575619 Msa0793670:CDS 40.0%
TGTTTAGAAGGGTCGACTAT+AGG - chr5_3:5575791-5575810 Msa0793670:intron 40.0%
GCAAGCAGACTTACAACAAA+AGG - chr5_3:5575819-5575838 Msa0793670:intron 40.0%
CTGGTAGCATTGTTTCCATT+AGG - chr5_3:5575923-5575942 Msa0793670:CDS 40.0%
GTAGCATTGTTTCCATTAGG+AGG - chr5_3:5575926-5575945 Msa0793670:CDS 40.0%
AACGTGAACAAACCTCCTAA+TGG + chr5_3:5575941-5575960 None:intergenic 40.0%
ACTCTCCAAATTCTACCGAG+AGG - chr5_3:5574910-5574929 Msa0793670:three_prime_UTR 45.0%
CAACACAACACTGACACATG+CGG - chr5_3:5574979-5574998 Msa0793670:three_prime_UTR 45.0%
CTTGAACTGTCCAAAGTCAG+GGG + chr5_3:5575133-5575152 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG + chr5_3:5575194-5575213 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG + chr5_3:5575235-5575254 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG + chr5_3:5575272-5575291 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG - chr5_3:5575603-5575622 Msa0793670:CDS 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG + chr5_3:5575817-5575836 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG - chr5_3:5575855-5575874 Msa0793670:intron 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG - chr5_3:5575904-5575923 Msa0793670:intron 45.0%
! GACGAGGTATCCATATTCCT+TGG + chr5_3:5576049-5576068 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG + chr5_3:5576065-5576084 None:intergenic 45.0%
ACTCTCCAAATTCTACCGAG+AGG - chr5_3:5574910-5574929 Msa0793670:three_prime_UTR 45.0%
CAACACAACACTGACACATG+CGG - chr5_3:5574979-5574998 Msa0793670:three_prime_UTR 45.0%
CTTGAACTGTCCAAAGTCAG+GGG + chr5_3:5575133-5575152 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG + chr5_3:5575194-5575213 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG + chr5_3:5575235-5575254 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG + chr5_3:5575272-5575291 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG - chr5_3:5575603-5575622 Msa0793670:CDS 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG + chr5_3:5575817-5575836 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG - chr5_3:5575855-5575874 Msa0793670:intron 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG - chr5_3:5575904-5575923 Msa0793670:intron 45.0%
! GACGAGGTATCCATATTCCT+TGG + chr5_3:5576049-5576068 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG + chr5_3:5576065-5576084 None:intergenic 45.0%
! GGAGAGTCTTTTTACGTCGG+TGG + chr5_3:5574897-5574916 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG + chr5_3:5574981-5575000 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG - chr5_3:5575120-5575139 Msa0793670:three_prime_UTR 50.0%
CATACAACGTAGCAACGAGC+TGG - chr5_3:5575197-5575216 Msa0793670:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG + chr5_3:5575252-5575271 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG + chr5_3:5575253-5575272 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG + chr5_3:5575263-5575282 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG - chr5_3:5575860-5575879 Msa0793670:intron 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG - chr5_3:5575861-5575880 Msa0793670:intron 50.0%
AATAAAGCCACCCAAACCGC+AGG + chr5_3:5575874-5575893 None:intergenic 50.0%
! GGAGAGTCTTTTTACGTCGG+TGG + chr5_3:5574897-5574916 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG + chr5_3:5574981-5575000 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG - chr5_3:5575120-5575139 Msa0793670:three_prime_UTR 50.0%
CATACAACGTAGCAACGAGC+TGG - chr5_3:5575197-5575216 Msa0793670:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG + chr5_3:5575252-5575271 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG + chr5_3:5575253-5575272 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG + chr5_3:5575263-5575282 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG - chr5_3:5575860-5575879 Msa0793670:intron 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG - chr5_3:5575861-5575880 Msa0793670:intron 50.0%
AATAAAGCCACCCAAACCGC+AGG + chr5_3:5575874-5575893 None:intergenic 50.0%
GAAGAAATGGCCGCAGCAGA+AGG - chr5_3:5574842-5574861 Msa0793670:three_prime_UTR 55.0%
CAGACTCACTCCTTCTGCTG+CGG + chr5_3:5574855-5574874 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG + chr5_3:5574880-5574899 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG - chr5_3:5575246-5575265 Msa0793670:intron 55.0%
GGCCTAAGCGGATATGAGAG+GGG + chr5_3:5575251-5575270 None:intergenic 55.0%
GAAGAAATGGCCGCAGCAGA+AGG - chr5_3:5574842-5574861 Msa0793670:three_prime_UTR 55.0%
CAGACTCACTCCTTCTGCTG+CGG + chr5_3:5574855-5574874 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG + chr5_3:5574880-5574899 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG - chr5_3:5575246-5575265 Msa0793670:intron 55.0%
GGCCTAAGCGGATATGAGAG+GGG + chr5_3:5575251-5575270 None:intergenic 55.0%
! TGTGTTGCCTGCGGTTTGGG+TGG - chr5_3:5575864-5575883 Msa0793670:intron 60.0%
! TGTGTTGCCTGCGGTTTGGG+TGG - chr5_3:5575864-5575883 Msa0793670:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 5574714 5576131 5574714 ID=Msa0793670;Name=Msa0793670
chr5_3 mRNA 5574714 5576131 5574714 ID=Msa0793670-mRNA-1;Parent=Msa0793670;Name=Msa0793670-mRNA-1;_AED=0.26;_eAED=0.26;_QI=134|1|1|1|1|1|4|477|138
chr5_3 exon 5574714 5575234 5574714 ID=Msa0793670-mRNA-1:exon:2188;Parent=Msa0793670-mRNA-1
chr5_3 exon 5575356 5575425 5575356 ID=Msa0793670-mRNA-1:exon:2187;Parent=Msa0793670-mRNA-1
chr5_3 exon 5575522 5575740 5575522 ID=Msa0793670-mRNA-1:exon:2186;Parent=Msa0793670-mRNA-1
chr5_3 exon 5575914 5576131 5575914 ID=Msa0793670-mRNA-1:exon:2185;Parent=Msa0793670-mRNA-1
chr5_3 five_prime_UTR 5575998 5576131 5575998 ID=Msa0793670-mRNA-1:five_prime_utr;Parent=Msa0793670-mRNA-1
chr5_3 CDS 5575914 5575997 5575914 ID=Msa0793670-mRNA-1:cds;Parent=Msa0793670-mRNA-1
chr5_3 CDS 5575522 5575740 5575522 ID=Msa0793670-mRNA-1:cds;Parent=Msa0793670-mRNA-1
chr5_3 CDS 5575356 5575425 5575356 ID=Msa0793670-mRNA-1:cds;Parent=Msa0793670-mRNA-1
chr5_3 CDS 5575191 5575234 5575191 ID=Msa0793670-mRNA-1:cds;Parent=Msa0793670-mRNA-1
chr5_3 three_prime_UTR 5574714 5575190 5574714 ID=Msa0793670-mRNA-1:three_prime_utr;Parent=Msa0793670-mRNA-1
Gene Sequence

>Msa0793670

ATGGCCGCAGCAGAAGGAGTGAGTCTGAACCATATAGCTCGAGAGTCCACCGACGTAAAAAGACTCTCCAAATTCTACCGAGAGATGTTTGGGTTTGAGGAAGTTGAGACCCCTGACTTTGGACAGTTCAAGATTATATGGTTGCGCCTTCCTTCTTCATCCTTCCTTATCCATCTCATACAACGTAGCAACGAGCTGGCTCCATCATCATCCATTCCTGTTAAAGACCCCTCTCATATCCGCTTAGGCCATCATCTCTGCTTCTCCATCTCCAATCTTCATTCCTTTCACAACACTCTCAAGGACAAAGGTATTGAGACTTTTGAGACTACTAATGGCAACATCAAACGAGTTTTCTTCTATGACCCTGATGGTAATGGCTTGGAGGTATTTGCTAGCATCGAAGACTCCTCCTAG
Protein sequence

>Msa0793670

MAAAEGVSLNHIARESTDVKRLSKFYREMFGFEEVETPDFGQFKIIWLRLPSSSFLIHLIQRSNELAPSSSIPVKDPSHIRLGHHLCFSISNLHSFHNTLKDKGIETFETTNGNIKRVFFYDPDGNGLEVFASIEDSS*