AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0829900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829900 MtrunA17_Chr5g0394521 94.964 139 6 1 1 138 1 139 8.28e-94 267
Msa0829900 MtrunA17_Chr5g0394511 66.906 139 38 4 6 137 3 140 6.72e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829900 AT2G32090.1 54.074 135 46 4 7 130 2 131 2.47e-50 157
Msa0829900 AT2G32090.2 52.212 113 38 4 29 130 2 109 7.12e-38 125
Msa0829900 AT2G28420.1 29.457 129 80 3 7 130 19 141 6.64e-12 60.5

Find 74 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTTATCCAACGAAATT+AGG 0.180713 5_4:+2899886 Msa0829900:five_prime_UTR
TCCTCCTAGTTACTTTAATT+TGG 0.200302 5_4:+2900780 Msa0829900:exon
ACTTACCTCTCGGTAGAATT+TGG 0.203336 5_4:-2900049 None:intergenic
AGAAAAGATTTCTATTGATT+TGG 0.249533 5_4:-2899941 None:intergenic
TATTGATTTGGAAACAATAA+TGG 0.294612 5_4:-2899929 None:intergenic
AAAGAAATTACCAAGGAATA+TGG 0.297272 5_4:+2901170 Msa0829900:three_prime_UTR
ACACAAACTACAGTAAAATT+AGG 0.312173 5_4:-2900978 None:intergenic
TTGGACAGTTCAAGATTATA+TGG 0.312649 5_4:+2900273 Msa0829900:CDS
TTGTGTGTTGCCTGCGGTTT+GGG 0.319401 5_4:+2900995 Msa0829900:three_prime_UTR
CACTACTTGTGATTTCTTGT+CGG 0.326553 5_4:+2900897 Msa0829900:three_prime_UTR
TGATTGAGTTATCAGATGTT+TGG 0.331923 5_4:+2900224 Msa0829900:intron
GATTGAGTTATCAGATGTTT+GGG 0.363031 5_4:+2900225 Msa0829900:intron
TGTTTAGAAGGGTCGACTAT+AGG 0.382836 5_4:+2900925 Msa0829900:three_prime_UTR
AGTCAAAGTTAATTAAGATA+TGG 0.385112 5_4:-2901243 None:intergenic
ATCTTGAACTGTCCAAAGTC+AGG 0.387838 5_4:-2900266 None:intergenic
CTGGTAGCATTGTTTCCATT+AGG 0.390280 5_4:+2901057 Msa0829900:three_prime_UTR
TTATCAGATGTTTGGGTTTG+AGG 0.401608 5_4:+2900232 Msa0829900:intron
ATATGAGAGGGGTCTTTAAC+AGG 0.405224 5_4:-2900371 None:intergenic
TTTGTGTGTTGCCTGCGGTT+TGG 0.409406 5_4:+2900994 Msa0829900:three_prime_UTR
CGGTGGACTCTCGAGCTATA+TGG 0.414336 5_4:-2900011 None:intergenic
TCTAGAAAAGATAGAAGAAA+TGG 0.416833 5_4:+2899963 Msa0829900:exon
ACTACTTGTGATTTCTTGTC+GGG 0.422987 5_4:+2900898 Msa0829900:three_prime_UTR
AACGTGAACAAACCTCCTAA+TGG 0.431030 5_4:-2901072 None:intergenic
TGTTGTAAGTCTGCTTGCAC+AGG 0.453222 5_4:-2900948 None:intergenic
GAAGTTGAGACCCCTGACTT+TGG 0.454246 5_4:+2900254 Msa0829900:CDS
TTTCAAATCATCACATGGGT+AGG 0.475491 5_4:+2899867 Msa0829900:five_prime_UTR
GTTGTGAAAGGAATGAAGAT+TGG 0.479221 5_4:-2900426 None:intergenic
CATTGTTTCAAATCATCACA+TGG 0.485619 5_4:+2899862 Msa0829900:five_prime_UTR
ATCTAGCACCCTAATTTCGT+TGG 0.488283 5_4:-2899895 None:intergenic
AAGACTAGGTTGATGATAGT+TGG 0.508283 5_4:-2900856 None:intergenic
AGATGGAGAAGCAGAGATGA+TGG 0.508377 5_4:-2900403 None:intergenic
GCAAGCAGACTTACAACAAA+AGG 0.511066 5_4:+2900953 Msa0829900:three_prime_UTR
TACCAGTTGACAAAATGAAA+GGG 0.515688 5_4:-2901040 None:intergenic
TGTATATGCAGGTAATGGCT+TGG 0.518965 5_4:+2900734 Msa0829900:intron
GACGAGGTATCCATATTCCT+TGG 0.519059 5_4:-2901180 None:intergenic
TTCAAATCATCACATGGGTA+GGG 0.520517 5_4:+2899868 Msa0829900:five_prime_UTR
CGTTGCTACGTTGTATGAGA+TGG 0.521284 5_4:-2900325 None:intergenic
AGAGGGGTCTTTAACAGGAA+TGG 0.522033 5_4:-2900366 None:intergenic
TTCCTTTCACAACACTCTCA+AGG 0.527218 5_4:+2900436 Msa0829900:CDS
AGGGTTATCCAACGAAATTA+GGG 0.528572 5_4:+2899887 Msa0829900:five_prime_UTR
GTAGCATTGTTTCCATTAGG+AGG 0.531970 5_4:+2901060 Msa0829900:three_prime_UTR
GAAGAAATGGCCGCAGCAGA+AGG 0.534245 5_4:+2899976 Msa0829900:exon
CTACCAGTTGACAAAATGAA+AGG 0.534782 5_4:-2901041 None:intergenic
TCTTGAACTGTCCAAAGTCA+GGG 0.541425 5_4:-2900265 None:intergenic
TGGATAAGGAAGGATGAAGA+AGG 0.546868 5_4:-2900305 None:intergenic
AATAAAGCCACCCAAACCGC+AGG 0.546895 5_4:-2901005 None:intergenic
GTCTTAATTAATTACCATCA+GGG 0.550683 5_4:-2900616 None:intergenic
TACGTTGTATGAGATGGATA+AGG 0.553336 5_4:-2900319 None:intergenic
TGATTTGAAACAATGAAAGA+TGG 0.554494 5_4:-2899854 None:intergenic
TTAACAGGAATGGATGATGA+TGG 0.555627 5_4:-2900356 None:intergenic
CAGTATCATTAATCAAGACT+AGG 0.565450 5_4:-2900870 None:intergenic
AACCTTGAGAGTGTTGTGAA+AGG 0.570775 5_4:-2900438 None:intergenic
AAAGGAATGAAGATTGGAGA+TGG 0.571006 5_4:-2900420 None:intergenic
ATATGCAGGTAATGGCTTGG+AGG 0.578557 5_4:+2900737 Msa0829900:intron
TGGCCTAAGCGGATATGAGA+GGG 0.586943 5_4:-2900383 None:intergenic
CATACAACGTAGCAACGAGC+TGG 0.597863 5_4:+2900331 Msa0829900:CDS
TTGTATGAGATGGATAAGGA+AGG 0.611019 5_4:-2900315 None:intergenic
TGTGTTGCCTGCGGTTTGGG+TGG 0.612077 5_4:+2900998 Msa0829900:three_prime_UTR
AGCAGAGATGATGGCCTAAG+CGG 0.614263 5_4:-2900394 None:intergenic
ATGGCCTAAGCGGATATGAG+AGG 0.629456 5_4:-2900384 None:intergenic
ATTGTTTCAAATCATCACAT+GGG 0.632503 5_4:+2899863 Msa0829900:five_prime_UTR
GACCCCTCTCATATCCGCTT+AGG 0.637582 5_4:+2900380 Msa0829900:CDS
ACCAGTTGACAAAATGAAAG+GGG 0.643412 5_4:-2901039 None:intergenic
TAAGGAAGGATGAAGAAGGA+AGG 0.647624 5_4:-2900301 None:intergenic
ACCAAATTAAAGTAACTAGG+AGG 0.648939 5_4:-2900781 None:intergenic
TGTAGTTTGTGTGTTGCCTG+CGG 0.657320 5_4:+2900989 Msa0829900:three_prime_UTR
GACACCAAATTAAAGTAACT+AGG 0.660293 5_4:-2900784 None:intergenic
CTTGAACTGTCCAAAGTCAG+GGG 0.685610 5_4:-2900264 None:intergenic
TGATTTGGAAACAATAATGG+TGG 0.685697 5_4:-2899926 None:intergenic
AAAGCTAAAAGAAATTACCA+AGG 0.689700 5_4:+2901163 Msa0829900:three_prime_UTR
CAGACTCACTCCTTCTGCTG+CGG 0.692086 5_4:-2899986 None:intergenic
GGCCTAAGCGGATATGAGAG+GGG 0.694889 5_4:-2900382 None:intergenic
GGACATCAAAGTAGATGACG+AGG 0.710844 5_4:-2901196 None:intergenic
ACTCTCCAAATTCTACCGAG+AGG 0.710906 5_4:+2900044 Msa0829900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAATTATTATCTGTG+TGG + chr5_4:2900152-2900171 Msa0829900:intron 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG - chr5_4:2901220-2901239 None:intergenic 15.0%
!!! TTTTTAAATTATTATCTGTG+TGG + chr5_4:2900152-2900171 Msa0829900:intron 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG - chr5_4:2901220-2901239 None:intergenic 15.0%
!!! TATTGATTTGGAAACAATAA+TGG - chr5_4:2899932-2899951 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG - chr5_4:2899944-2899963 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG - chr5_4:2901246-2901265 None:intergenic 20.0%
!!! TATTGATTTGGAAACAATAA+TGG - chr5_4:2899932-2899951 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG - chr5_4:2899944-2899963 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG - chr5_4:2901246-2901265 None:intergenic 20.0%
!! TGATTTGAAACAATGAAAGA+TGG - chr5_4:2899857-2899876 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG + chr5_4:2899863-2899882 Msa0829900:five_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG + chr5_4:2899963-2899982 Msa0829900:exon 25.0%
! TTAAATACTAACTTACCTCT+CGG - chr5_4:2900062-2900081 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG + chr5_4:2900532-2900551 Msa0829900:intron 25.0%
!!! CATTTTTTTATACAGGACAA+AGG + chr5_4:2900539-2900558 Msa0829900:intron 25.0%
! GTCTTAATTAATTACCATCA+GGG - chr5_4:2900619-2900638 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG - chr5_4:2900620-2900639 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG + chr5_4:2900680-2900699 Msa0829900:intron 25.0%
! GTAGTATTAATTGTATATGC+AGG + chr5_4:2900723-2900742 Msa0829900:intron 25.0%
! TTAATTGTATATGCAGGTAA+TGG + chr5_4:2900729-2900748 Msa0829900:intron 25.0%
! ACACAAACTACAGTAAAATT+AGG - chr5_4:2900981-2901000 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG - chr5_4:2901164-2901183 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG + chr5_4:2901163-2901182 Msa0829900:three_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG + chr5_4:2901170-2901189 Msa0829900:three_prime_UTR 25.0%
!! TGATTTGAAACAATGAAAGA+TGG - chr5_4:2899857-2899876 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG + chr5_4:2899863-2899882 Msa0829900:five_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG + chr5_4:2899963-2899982 Msa0829900:exon 25.0%
! TTAAATACTAACTTACCTCT+CGG - chr5_4:2900062-2900081 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG + chr5_4:2900532-2900551 Msa0829900:intron 25.0%
!!! CATTTTTTTATACAGGACAA+AGG + chr5_4:2900539-2900558 Msa0829900:intron 25.0%
! GTCTTAATTAATTACCATCA+GGG - chr5_4:2900619-2900638 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG - chr5_4:2900620-2900639 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG + chr5_4:2900680-2900699 Msa0829900:intron 25.0%
! GTAGTATTAATTGTATATGC+AGG + chr5_4:2900723-2900742 Msa0829900:intron 25.0%
! TTAATTGTATATGCAGGTAA+TGG + chr5_4:2900729-2900748 Msa0829900:intron 25.0%
! ACACAAACTACAGTAAAATT+AGG - chr5_4:2900981-2901000 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG - chr5_4:2901164-2901183 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG + chr5_4:2901163-2901182 Msa0829900:three_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG + chr5_4:2901170-2901189 Msa0829900:three_prime_UTR 25.0%
CATTGTTTCAAATCATCACA+TGG + chr5_4:2899862-2899881 Msa0829900:five_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG - chr5_4:2899929-2899948 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG - chr5_4:2900203-2900222 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG + chr5_4:2900224-2900243 Msa0829900:intron 30.0%
GATTGAGTTATCAGATGTTT+GGG + chr5_4:2900225-2900244 Msa0829900:intron 30.0%
TTGGACAGTTCAAGATTATA+TGG + chr5_4:2900273-2900292 Msa0829900:CDS 30.0%
AAACTCAAATCTAACAACAG+TGG - chr5_4:2900481-2900500 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG + chr5_4:2900780-2900799 Msa0829900:exon 30.0%
ACCAAATTAAAGTAACTAGG+AGG - chr5_4:2900784-2900803 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG - chr5_4:2900787-2900806 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG - chr5_4:2900873-2900892 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG + chr5_4:2901016-2901035 Msa0829900:three_prime_UTR 30.0%
TACCAGTTGACAAAATGAAA+GGG - chr5_4:2901043-2901062 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG - chr5_4:2901156-2901175 None:intergenic 30.0%
CATTGTTTCAAATCATCACA+TGG + chr5_4:2899862-2899881 Msa0829900:five_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG - chr5_4:2899929-2899948 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG - chr5_4:2900203-2900222 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG + chr5_4:2900224-2900243 Msa0829900:intron 30.0%
GATTGAGTTATCAGATGTTT+GGG + chr5_4:2900225-2900244 Msa0829900:intron 30.0%
TTGGACAGTTCAAGATTATA+TGG + chr5_4:2900273-2900292 Msa0829900:CDS 30.0%
AAACTCAAATCTAACAACAG+TGG - chr5_4:2900481-2900500 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG + chr5_4:2900780-2900799 Msa0829900:exon 30.0%
ACCAAATTAAAGTAACTAGG+AGG - chr5_4:2900784-2900803 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG - chr5_4:2900787-2900806 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG - chr5_4:2900873-2900892 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG + chr5_4:2901016-2901035 Msa0829900:three_prime_UTR 30.0%
TACCAGTTGACAAAATGAAA+GGG - chr5_4:2901043-2901062 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG - chr5_4:2901156-2901175 None:intergenic 30.0%
TTTCAAATCATCACATGGGT+AGG + chr5_4:2899867-2899886 Msa0829900:five_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG + chr5_4:2899868-2899887 Msa0829900:five_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG + chr5_4:2899886-2899905 Msa0829900:five_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG + chr5_4:2899887-2899906 Msa0829900:five_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG - chr5_4:2900034-2900053 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG + chr5_4:2900232-2900251 Msa0829900:intron 35.0%
TTGTATGAGATGGATAAGGA+AGG - chr5_4:2900318-2900337 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG - chr5_4:2900322-2900341 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG - chr5_4:2900359-2900378 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG - chr5_4:2900423-2900442 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG - chr5_4:2900429-2900448 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG + chr5_4:2900566-2900585 Msa0829900:CDS 35.0%
TACGTGATCATCTCATCTTA+GGG - chr5_4:2900706-2900725 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG - chr5_4:2900859-2900878 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG + chr5_4:2900897-2900916 Msa0829900:three_prime_UTR 35.0%
ACTACTTGTGATTTCTTGTC+GGG + chr5_4:2900898-2900917 Msa0829900:three_prime_UTR 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG + chr5_4:2900913-2900932 Msa0829900:three_prime_UTR 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG + chr5_4:2900914-2900933 Msa0829900:three_prime_UTR 35.0%
ACCAGTTGACAAAATGAAAG+GGG - chr5_4:2901042-2901061 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG - chr5_4:2901044-2901063 None:intergenic 35.0%
TTTCAAATCATCACATGGGT+AGG + chr5_4:2899867-2899886 Msa0829900:five_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG + chr5_4:2899868-2899887 Msa0829900:five_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG + chr5_4:2899886-2899905 Msa0829900:five_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG + chr5_4:2899887-2899906 Msa0829900:five_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG - chr5_4:2900034-2900053 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG + chr5_4:2900232-2900251 Msa0829900:intron 35.0%
TTGTATGAGATGGATAAGGA+AGG - chr5_4:2900318-2900337 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG - chr5_4:2900322-2900341 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG - chr5_4:2900359-2900378 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG - chr5_4:2900423-2900442 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG - chr5_4:2900429-2900448 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG + chr5_4:2900566-2900585 Msa0829900:CDS 35.0%
TACGTGATCATCTCATCTTA+GGG - chr5_4:2900706-2900725 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG - chr5_4:2900859-2900878 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG + chr5_4:2900897-2900916 Msa0829900:three_prime_UTR 35.0%
ACTACTTGTGATTTCTTGTC+GGG + chr5_4:2900898-2900917 Msa0829900:three_prime_UTR 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG + chr5_4:2900913-2900932 Msa0829900:three_prime_UTR 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG + chr5_4:2900914-2900933 Msa0829900:three_prime_UTR 35.0%
ACCAGTTGACAAAATGAAAG+GGG - chr5_4:2901042-2901061 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG - chr5_4:2901044-2901063 None:intergenic 35.0%
ATCTAGCACCCTAATTTCGT+TGG - chr5_4:2899898-2899917 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG - chr5_4:2900052-2900071 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG - chr5_4:2900085-2900104 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG - chr5_4:2900183-2900202 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG - chr5_4:2900268-2900287 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG - chr5_4:2900269-2900288 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG - chr5_4:2900304-2900323 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG - chr5_4:2900308-2900327 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG - chr5_4:2900374-2900393 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG + chr5_4:2900436-2900455 Msa0829900:CDS 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG - chr5_4:2900441-2900460 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG + chr5_4:2900602-2900621 Msa0829900:CDS 40.0%
CATCTTAGGGCCAATAAACA+AGG - chr5_4:2900693-2900712 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG - chr5_4:2900707-2900726 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG + chr5_4:2900734-2900753 Msa0829900:intron 40.0%
TGTTTAGAAGGGTCGACTAT+AGG + chr5_4:2900925-2900944 Msa0829900:three_prime_UTR 40.0%
GCAAGCAGACTTACAACAAA+AGG + chr5_4:2900953-2900972 Msa0829900:three_prime_UTR 40.0%
CTGGTAGCATTGTTTCCATT+AGG + chr5_4:2901057-2901076 Msa0829900:three_prime_UTR 40.0%
GTAGCATTGTTTCCATTAGG+AGG + chr5_4:2901060-2901079 Msa0829900:three_prime_UTR 40.0%
AACGTGAACAAACCTCCTAA+TGG - chr5_4:2901075-2901094 None:intergenic 40.0%
ATCTAGCACCCTAATTTCGT+TGG - chr5_4:2899898-2899917 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG - chr5_4:2900052-2900071 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG - chr5_4:2900085-2900104 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG - chr5_4:2900183-2900202 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG - chr5_4:2900268-2900287 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG - chr5_4:2900269-2900288 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG - chr5_4:2900304-2900323 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG - chr5_4:2900308-2900327 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG - chr5_4:2900374-2900393 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG + chr5_4:2900436-2900455 Msa0829900:CDS 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG - chr5_4:2900441-2900460 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG + chr5_4:2900602-2900621 Msa0829900:CDS 40.0%
CATCTTAGGGCCAATAAACA+AGG - chr5_4:2900693-2900712 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG - chr5_4:2900707-2900726 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG + chr5_4:2900734-2900753 Msa0829900:intron 40.0%
TGTTTAGAAGGGTCGACTAT+AGG + chr5_4:2900925-2900944 Msa0829900:three_prime_UTR 40.0%
GCAAGCAGACTTACAACAAA+AGG + chr5_4:2900953-2900972 Msa0829900:three_prime_UTR 40.0%
CTGGTAGCATTGTTTCCATT+AGG + chr5_4:2901057-2901076 Msa0829900:three_prime_UTR 40.0%
GTAGCATTGTTTCCATTAGG+AGG + chr5_4:2901060-2901079 Msa0829900:three_prime_UTR 40.0%
AACGTGAACAAACCTCCTAA+TGG - chr5_4:2901075-2901094 None:intergenic 40.0%
ACTCTCCAAATTCTACCGAG+AGG + chr5_4:2900044-2900063 Msa0829900:CDS 45.0%
CAACACAACACTGACACATG+CGG + chr5_4:2900113-2900132 Msa0829900:intron 45.0%
CTTGAACTGTCCAAAGTCAG+GGG - chr5_4:2900267-2900286 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG - chr5_4:2900328-2900347 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG - chr5_4:2900369-2900388 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG - chr5_4:2900406-2900425 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG + chr5_4:2900737-2900756 Msa0829900:intron 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG - chr5_4:2900951-2900970 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG + chr5_4:2900989-2901008 Msa0829900:three_prime_UTR 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG + chr5_4:2901038-2901057 Msa0829900:three_prime_UTR 45.0%
! GACGAGGTATCCATATTCCT+TGG - chr5_4:2901183-2901202 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG - chr5_4:2901199-2901218 None:intergenic 45.0%
ACTCTCCAAATTCTACCGAG+AGG + chr5_4:2900044-2900063 Msa0829900:CDS 45.0%
CAACACAACACTGACACATG+CGG + chr5_4:2900113-2900132 Msa0829900:intron 45.0%
CTTGAACTGTCCAAAGTCAG+GGG - chr5_4:2900267-2900286 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG - chr5_4:2900328-2900347 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG - chr5_4:2900369-2900388 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG - chr5_4:2900406-2900425 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG + chr5_4:2900737-2900756 Msa0829900:intron 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG - chr5_4:2900951-2900970 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG + chr5_4:2900989-2901008 Msa0829900:three_prime_UTR 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG + chr5_4:2901038-2901057 Msa0829900:three_prime_UTR 45.0%
! GACGAGGTATCCATATTCCT+TGG - chr5_4:2901183-2901202 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG - chr5_4:2901199-2901218 None:intergenic 45.0%
! GGAGAGTCTTTTTACGTCGG+TGG - chr5_4:2900031-2900050 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG - chr5_4:2900115-2900134 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG + chr5_4:2900254-2900273 Msa0829900:CDS 50.0%
CATACAACGTAGCAACGAGC+TGG + chr5_4:2900331-2900350 Msa0829900:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG - chr5_4:2900386-2900405 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG - chr5_4:2900387-2900406 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG - chr5_4:2900397-2900416 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG + chr5_4:2900994-2901013 Msa0829900:three_prime_UTR 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG + chr5_4:2900995-2901014 Msa0829900:three_prime_UTR 50.0%
AATAAAGCCACCCAAACCGC+AGG - chr5_4:2901008-2901027 None:intergenic 50.0%
! GGAGAGTCTTTTTACGTCGG+TGG - chr5_4:2900031-2900050 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG - chr5_4:2900115-2900134 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG + chr5_4:2900254-2900273 Msa0829900:CDS 50.0%
CATACAACGTAGCAACGAGC+TGG + chr5_4:2900331-2900350 Msa0829900:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG - chr5_4:2900386-2900405 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG - chr5_4:2900387-2900406 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG - chr5_4:2900397-2900416 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG + chr5_4:2900994-2901013 Msa0829900:three_prime_UTR 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG + chr5_4:2900995-2901014 Msa0829900:three_prime_UTR 50.0%
AATAAAGCCACCCAAACCGC+AGG - chr5_4:2901008-2901027 None:intergenic 50.0%
GAAGAAATGGCCGCAGCAGA+AGG + chr5_4:2899976-2899995 Msa0829900:exon 55.0%
CAGACTCACTCCTTCTGCTG+CGG - chr5_4:2899989-2900008 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG - chr5_4:2900014-2900033 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG + chr5_4:2900380-2900399 Msa0829900:CDS 55.0%
GGCCTAAGCGGATATGAGAG+GGG - chr5_4:2900385-2900404 None:intergenic 55.0%
GAAGAAATGGCCGCAGCAGA+AGG + chr5_4:2899976-2899995 Msa0829900:exon 55.0%
CAGACTCACTCCTTCTGCTG+CGG - chr5_4:2899989-2900008 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG - chr5_4:2900014-2900033 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG + chr5_4:2900380-2900399 Msa0829900:CDS 55.0%
GGCCTAAGCGGATATGAGAG+GGG - chr5_4:2900385-2900404 None:intergenic 55.0%
! TGTGTTGCCTGCGGTTTGGG+TGG + chr5_4:2900998-2901017 Msa0829900:three_prime_UTR 60.0%
! TGTGTTGCCTGCGGTTTGGG+TGG + chr5_4:2900998-2901017 Msa0829900:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 2899848 2901265 2899848 ID=Msa0829900;Name=Msa0829900
chr5_4 mRNA 2899848 2901265 2899848 ID=Msa0829900-mRNA-1;Parent=Msa0829900;Name=Msa0829900-mRNA-1;_AED=0.26;_eAED=0.26;_QI=134|1|1|1|1|1|4|477|138
chr5_4 exon 2899848 2900065 2899848 ID=Msa0829900-mRNA-1:exon:1053;Parent=Msa0829900-mRNA-1
chr5_4 exon 2900239 2900457 2900239 ID=Msa0829900-mRNA-1:exon:1054;Parent=Msa0829900-mRNA-1
chr5_4 exon 2900554 2900623 2900554 ID=Msa0829900-mRNA-1:exon:1055;Parent=Msa0829900-mRNA-1
chr5_4 exon 2900745 2901265 2900745 ID=Msa0829900-mRNA-1:exon:1056;Parent=Msa0829900-mRNA-1
chr5_4 five_prime_UTR 2899848 2899981 2899848 ID=Msa0829900-mRNA-1:five_prime_utr;Parent=Msa0829900-mRNA-1
chr5_4 CDS 2899982 2900065 2899982 ID=Msa0829900-mRNA-1:cds;Parent=Msa0829900-mRNA-1
chr5_4 CDS 2900239 2900457 2900239 ID=Msa0829900-mRNA-1:cds;Parent=Msa0829900-mRNA-1
chr5_4 CDS 2900554 2900623 2900554 ID=Msa0829900-mRNA-1:cds;Parent=Msa0829900-mRNA-1
chr5_4 CDS 2900745 2900788 2900745 ID=Msa0829900-mRNA-1:cds;Parent=Msa0829900-mRNA-1
chr5_4 three_prime_UTR 2900789 2901265 2900789 ID=Msa0829900-mRNA-1:three_prime_utr;Parent=Msa0829900-mRNA-1
Gene Sequence

>Msa0829900

ATGGCCGCAGCAGAAGGAGTGAGTCTGAACCATATAGCTCGAGAGTCCACCGACGTAAAAAGACTCTCCAAATTCTACCGAGAGATGTTTGGGTTTGAGGAAGTTGAGACCCCTGACTTTGGACAGTTCAAGATTATATGGTTGCGCCTTCCTTCTTCATCCTTCCTTATCCATCTCATACAACGTAGCAACGAGCTGGCTCCATCATCATCCATTCCTGTTAAAGACCCCTCTCATATCCGCTTAGGCCATCATCTCTGCTTCTCCATCTCCAATCTTCATTCCTTTCACAACACTCTCAAGGACAAAGGTATTGAGACTTTTGAGACTACTAATGGCAACATCAAACGAGTTTTCTTCTATGACCCTGATGGTAATGGCTTGGAGGTATTTGCTAGCATCGAAGACTCCTCCTAG
Protein sequence

>Msa0829900

MAAAEGVSLNHIARESTDVKRLSKFYREMFGFEEVETPDFGQFKIIWLRLPSSSFLIHLIQRSNELAPSSSIPVKDPSHIRLGHHLCFSISNLHSFHNTLKDKGIETFETTNGNIKRVFFYDPDGNGLEVFASIEDSS*