AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1071170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1071170 MtrunA17_Chr5g0394521 94.964 139 6 1 1 138 1 139 8.28e-94 267
Msa1071170 MtrunA17_Chr5g0394511 66.906 139 38 4 6 137 3 140 6.72e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1071170 AT2G32090.1 54.074 135 46 4 7 130 2 131 2.47e-50 157
Msa1071170 AT2G32090.2 52.212 113 38 4 29 130 2 109 7.12e-38 125
Msa1071170 AT2G28420.1 29.457 129 80 3 7 130 19 141 6.64e-12 60.5

Find 74 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTTATCCAACGAAATT+AGG 0.180713 7_2:+67635449 Msa1071170:five_prime_UTR
TCCTCCTAGTTACTTTAATT+TGG 0.200302 7_2:+67636343 Msa1071170:exon
ACTTACCTCTCGGTAGAATT+TGG 0.203336 7_2:-67635612 None:intergenic
AGAAAAGATTTCTATTGATT+TGG 0.249533 7_2:-67635504 None:intergenic
TATTGATTTGGAAACAATAA+TGG 0.294612 7_2:-67635492 None:intergenic
AAAGAAATTACCAAGGAATA+TGG 0.297272 7_2:+67636733 Msa1071170:three_prime_UTR
ACACAAACTACAGTAAAATT+AGG 0.312173 7_2:-67636541 None:intergenic
TTGGACAGTTCAAGATTATA+TGG 0.312649 7_2:+67635836 Msa1071170:CDS
TTGTGTGTTGCCTGCGGTTT+GGG 0.319401 7_2:+67636558 Msa1071170:three_prime_UTR
CACTACTTGTGATTTCTTGT+CGG 0.326553 7_2:+67636460 Msa1071170:three_prime_UTR
TGATTGAGTTATCAGATGTT+TGG 0.331923 7_2:+67635787 Msa1071170:intron
GATTGAGTTATCAGATGTTT+GGG 0.363031 7_2:+67635788 Msa1071170:intron
TGTTTAGAAGGGTCGACTAT+AGG 0.382836 7_2:+67636488 Msa1071170:three_prime_UTR
AGTCAAAGTTAATTAAGATA+TGG 0.385112 7_2:-67636806 None:intergenic
ATCTTGAACTGTCCAAAGTC+AGG 0.387838 7_2:-67635829 None:intergenic
CTGGTAGCATTGTTTCCATT+AGG 0.390280 7_2:+67636620 Msa1071170:three_prime_UTR
TTATCAGATGTTTGGGTTTG+AGG 0.401608 7_2:+67635795 Msa1071170:intron
ATATGAGAGGGGTCTTTAAC+AGG 0.405224 7_2:-67635934 None:intergenic
TTTGTGTGTTGCCTGCGGTT+TGG 0.409406 7_2:+67636557 Msa1071170:three_prime_UTR
CGGTGGACTCTCGAGCTATA+TGG 0.414336 7_2:-67635574 None:intergenic
TCTAGAAAAGATAGAAGAAA+TGG 0.416833 7_2:+67635526 Msa1071170:exon
ACTACTTGTGATTTCTTGTC+GGG 0.422987 7_2:+67636461 Msa1071170:three_prime_UTR
AACGTGAACAAACCTCCTAA+TGG 0.431030 7_2:-67636635 None:intergenic
TGTTGTAAGTCTGCTTGCAC+AGG 0.453222 7_2:-67636511 None:intergenic
GAAGTTGAGACCCCTGACTT+TGG 0.454246 7_2:+67635817 Msa1071170:CDS
TTTCAAATCATCACATGGGT+AGG 0.475491 7_2:+67635430 Msa1071170:five_prime_UTR
GTTGTGAAAGGAATGAAGAT+TGG 0.479221 7_2:-67635989 None:intergenic
CATTGTTTCAAATCATCACA+TGG 0.485619 7_2:+67635425 Msa1071170:five_prime_UTR
ATCTAGCACCCTAATTTCGT+TGG 0.488283 7_2:-67635458 None:intergenic
AAGACTAGGTTGATGATAGT+TGG 0.508283 7_2:-67636419 None:intergenic
AGATGGAGAAGCAGAGATGA+TGG 0.508377 7_2:-67635966 None:intergenic
GCAAGCAGACTTACAACAAA+AGG 0.511066 7_2:+67636516 Msa1071170:three_prime_UTR
TACCAGTTGACAAAATGAAA+GGG 0.515688 7_2:-67636603 None:intergenic
TGTATATGCAGGTAATGGCT+TGG 0.518965 7_2:+67636297 Msa1071170:intron
GACGAGGTATCCATATTCCT+TGG 0.519059 7_2:-67636743 None:intergenic
TTCAAATCATCACATGGGTA+GGG 0.520517 7_2:+67635431 Msa1071170:five_prime_UTR
CGTTGCTACGTTGTATGAGA+TGG 0.521284 7_2:-67635888 None:intergenic
AGAGGGGTCTTTAACAGGAA+TGG 0.522033 7_2:-67635929 None:intergenic
TTCCTTTCACAACACTCTCA+AGG 0.527218 7_2:+67635999 Msa1071170:CDS
AGGGTTATCCAACGAAATTA+GGG 0.528572 7_2:+67635450 Msa1071170:five_prime_UTR
GTAGCATTGTTTCCATTAGG+AGG 0.531970 7_2:+67636623 Msa1071170:three_prime_UTR
GAAGAAATGGCCGCAGCAGA+AGG 0.534245 7_2:+67635539 Msa1071170:exon
CTACCAGTTGACAAAATGAA+AGG 0.534782 7_2:-67636604 None:intergenic
TCTTGAACTGTCCAAAGTCA+GGG 0.541425 7_2:-67635828 None:intergenic
TGGATAAGGAAGGATGAAGA+AGG 0.546868 7_2:-67635868 None:intergenic
AATAAAGCCACCCAAACCGC+AGG 0.546895 7_2:-67636568 None:intergenic
GTCTTAATTAATTACCATCA+GGG 0.550683 7_2:-67636179 None:intergenic
TACGTTGTATGAGATGGATA+AGG 0.553336 7_2:-67635882 None:intergenic
TGATTTGAAACAATGAAAGA+TGG 0.554494 7_2:-67635417 None:intergenic
TTAACAGGAATGGATGATGA+TGG 0.555627 7_2:-67635919 None:intergenic
CAGTATCATTAATCAAGACT+AGG 0.565450 7_2:-67636433 None:intergenic
AACCTTGAGAGTGTTGTGAA+AGG 0.570775 7_2:-67636001 None:intergenic
AAAGGAATGAAGATTGGAGA+TGG 0.571006 7_2:-67635983 None:intergenic
ATATGCAGGTAATGGCTTGG+AGG 0.578557 7_2:+67636300 Msa1071170:intron
TGGCCTAAGCGGATATGAGA+GGG 0.586943 7_2:-67635946 None:intergenic
CATACAACGTAGCAACGAGC+TGG 0.597863 7_2:+67635894 Msa1071170:CDS
TTGTATGAGATGGATAAGGA+AGG 0.611019 7_2:-67635878 None:intergenic
TGTGTTGCCTGCGGTTTGGG+TGG 0.612077 7_2:+67636561 Msa1071170:three_prime_UTR
AGCAGAGATGATGGCCTAAG+CGG 0.614263 7_2:-67635957 None:intergenic
ATGGCCTAAGCGGATATGAG+AGG 0.629456 7_2:-67635947 None:intergenic
ATTGTTTCAAATCATCACAT+GGG 0.632503 7_2:+67635426 Msa1071170:five_prime_UTR
GACCCCTCTCATATCCGCTT+AGG 0.637582 7_2:+67635943 Msa1071170:CDS
ACCAGTTGACAAAATGAAAG+GGG 0.643412 7_2:-67636602 None:intergenic
TAAGGAAGGATGAAGAAGGA+AGG 0.647624 7_2:-67635864 None:intergenic
ACCAAATTAAAGTAACTAGG+AGG 0.648939 7_2:-67636344 None:intergenic
TGTAGTTTGTGTGTTGCCTG+CGG 0.657320 7_2:+67636552 Msa1071170:three_prime_UTR
GACACCAAATTAAAGTAACT+AGG 0.660293 7_2:-67636347 None:intergenic
CTTGAACTGTCCAAAGTCAG+GGG 0.685610 7_2:-67635827 None:intergenic
TGATTTGGAAACAATAATGG+TGG 0.685697 7_2:-67635489 None:intergenic
AAAGCTAAAAGAAATTACCA+AGG 0.689700 7_2:+67636726 Msa1071170:three_prime_UTR
CAGACTCACTCCTTCTGCTG+CGG 0.692086 7_2:-67635549 None:intergenic
GGCCTAAGCGGATATGAGAG+GGG 0.694889 7_2:-67635945 None:intergenic
GGACATCAAAGTAGATGACG+AGG 0.710844 7_2:-67636759 None:intergenic
ACTCTCCAAATTCTACCGAG+AGG 0.710906 7_2:+67635607 Msa1071170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAATTATTATCTGTG+TGG + chr7_2:67635715-67635734 Msa1071170:intron 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG - chr7_2:67636783-67636802 None:intergenic 15.0%
!!! TTTTTAAATTATTATCTGTG+TGG + chr7_2:67635715-67635734 Msa1071170:intron 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG - chr7_2:67636783-67636802 None:intergenic 15.0%
!!! TATTGATTTGGAAACAATAA+TGG - chr7_2:67635495-67635514 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG - chr7_2:67635507-67635526 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG - chr7_2:67636809-67636828 None:intergenic 20.0%
!!! TATTGATTTGGAAACAATAA+TGG - chr7_2:67635495-67635514 None:intergenic 20.0%
!!! AGAAAAGATTTCTATTGATT+TGG - chr7_2:67635507-67635526 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG - chr7_2:67636809-67636828 None:intergenic 20.0%
!! TGATTTGAAACAATGAAAGA+TGG - chr7_2:67635420-67635439 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG + chr7_2:67635426-67635445 Msa1071170:five_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG + chr7_2:67635526-67635545 Msa1071170:exon 25.0%
! TTAAATACTAACTTACCTCT+CGG - chr7_2:67635625-67635644 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG + chr7_2:67636095-67636114 Msa1071170:intron 25.0%
!!! CATTTTTTTATACAGGACAA+AGG + chr7_2:67636102-67636121 Msa1071170:intron 25.0%
! GTCTTAATTAATTACCATCA+GGG - chr7_2:67636182-67636201 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG - chr7_2:67636183-67636202 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG + chr7_2:67636243-67636262 Msa1071170:intron 25.0%
! GTAGTATTAATTGTATATGC+AGG + chr7_2:67636286-67636305 Msa1071170:intron 25.0%
! TTAATTGTATATGCAGGTAA+TGG + chr7_2:67636292-67636311 Msa1071170:intron 25.0%
! ACACAAACTACAGTAAAATT+AGG - chr7_2:67636544-67636563 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG - chr7_2:67636727-67636746 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG + chr7_2:67636726-67636745 Msa1071170:three_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG + chr7_2:67636733-67636752 Msa1071170:three_prime_UTR 25.0%
!! TGATTTGAAACAATGAAAGA+TGG - chr7_2:67635420-67635439 None:intergenic 25.0%
! ATTGTTTCAAATCATCACAT+GGG + chr7_2:67635426-67635445 Msa1071170:five_prime_UTR 25.0%
! TCTAGAAAAGATAGAAGAAA+TGG + chr7_2:67635526-67635545 Msa1071170:exon 25.0%
! TTAAATACTAACTTACCTCT+CGG - chr7_2:67635625-67635644 None:intergenic 25.0%
!!! CACAAGTCATTTTTTTATAC+AGG + chr7_2:67636095-67636114 Msa1071170:intron 25.0%
!!! CATTTTTTTATACAGGACAA+AGG + chr7_2:67636102-67636121 Msa1071170:intron 25.0%
! GTCTTAATTAATTACCATCA+GGG - chr7_2:67636182-67636201 None:intergenic 25.0%
! AGTCTTAATTAATTACCATC+AGG - chr7_2:67636183-67636202 None:intergenic 25.0%
!!! GTTATTAATGCCTTGTTTAT+TGG + chr7_2:67636243-67636262 Msa1071170:intron 25.0%
! GTAGTATTAATTGTATATGC+AGG + chr7_2:67636286-67636305 Msa1071170:intron 25.0%
! TTAATTGTATATGCAGGTAA+TGG + chr7_2:67636292-67636311 Msa1071170:intron 25.0%
! ACACAAACTACAGTAAAATT+AGG - chr7_2:67636544-67636563 None:intergenic 25.0%
!!! TTGGTAATTTCTTTTAGCTT+TGG - chr7_2:67636727-67636746 None:intergenic 25.0%
! AAAGCTAAAAGAAATTACCA+AGG + chr7_2:67636726-67636745 Msa1071170:three_prime_UTR 25.0%
! AAAGAAATTACCAAGGAATA+TGG + chr7_2:67636733-67636752 Msa1071170:three_prime_UTR 25.0%
CATTGTTTCAAATCATCACA+TGG + chr7_2:67635425-67635444 Msa1071170:five_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG - chr7_2:67635492-67635511 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG - chr7_2:67635766-67635785 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG + chr7_2:67635787-67635806 Msa1071170:intron 30.0%
GATTGAGTTATCAGATGTTT+GGG + chr7_2:67635788-67635807 Msa1071170:intron 30.0%
TTGGACAGTTCAAGATTATA+TGG + chr7_2:67635836-67635855 Msa1071170:CDS 30.0%
AAACTCAAATCTAACAACAG+TGG - chr7_2:67636044-67636063 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG + chr7_2:67636343-67636362 Msa1071170:exon 30.0%
ACCAAATTAAAGTAACTAGG+AGG - chr7_2:67636347-67636366 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG - chr7_2:67636350-67636369 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG - chr7_2:67636436-67636455 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG + chr7_2:67636579-67636598 Msa1071170:three_prime_UTR 30.0%
TACCAGTTGACAAAATGAAA+GGG - chr7_2:67636606-67636625 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG - chr7_2:67636719-67636738 None:intergenic 30.0%
CATTGTTTCAAATCATCACA+TGG + chr7_2:67635425-67635444 Msa1071170:five_prime_UTR 30.0%
! TGATTTGGAAACAATAATGG+TGG - chr7_2:67635492-67635511 None:intergenic 30.0%
ATTAACAAGTGATATGAAGC+AGG - chr7_2:67635766-67635785 None:intergenic 30.0%
TGATTGAGTTATCAGATGTT+TGG + chr7_2:67635787-67635806 Msa1071170:intron 30.0%
GATTGAGTTATCAGATGTTT+GGG + chr7_2:67635788-67635807 Msa1071170:intron 30.0%
TTGGACAGTTCAAGATTATA+TGG + chr7_2:67635836-67635855 Msa1071170:CDS 30.0%
AAACTCAAATCTAACAACAG+TGG - chr7_2:67636044-67636063 None:intergenic 30.0%
! TCCTCCTAGTTACTTTAATT+TGG + chr7_2:67636343-67636362 Msa1071170:exon 30.0%
ACCAAATTAAAGTAACTAGG+AGG - chr7_2:67636347-67636366 None:intergenic 30.0%
GACACCAAATTAAAGTAACT+AGG - chr7_2:67636350-67636369 None:intergenic 30.0%
CAGTATCATTAATCAAGACT+AGG - chr7_2:67636436-67636455 None:intergenic 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG + chr7_2:67636579-67636598 Msa1071170:three_prime_UTR 30.0%
TACCAGTTGACAAAATGAAA+GGG - chr7_2:67636606-67636625 None:intergenic 30.0%
!!! TTCTTTTAGCTTTGGTGATA+AGG - chr7_2:67636719-67636738 None:intergenic 30.0%
TTTCAAATCATCACATGGGT+AGG + chr7_2:67635430-67635449 Msa1071170:five_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG + chr7_2:67635431-67635450 Msa1071170:five_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG + chr7_2:67635449-67635468 Msa1071170:five_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG + chr7_2:67635450-67635469 Msa1071170:five_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG - chr7_2:67635597-67635616 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG + chr7_2:67635795-67635814 Msa1071170:intron 35.0%
TTGTATGAGATGGATAAGGA+AGG - chr7_2:67635881-67635900 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG - chr7_2:67635885-67635904 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG - chr7_2:67635922-67635941 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG - chr7_2:67635986-67636005 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG - chr7_2:67635992-67636011 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG + chr7_2:67636129-67636148 Msa1071170:CDS 35.0%
TACGTGATCATCTCATCTTA+GGG - chr7_2:67636269-67636288 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG - chr7_2:67636422-67636441 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG + chr7_2:67636460-67636479 Msa1071170:three_prime_UTR 35.0%
ACTACTTGTGATTTCTTGTC+GGG + chr7_2:67636461-67636480 Msa1071170:three_prime_UTR 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG + chr7_2:67636476-67636495 Msa1071170:three_prime_UTR 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG + chr7_2:67636477-67636496 Msa1071170:three_prime_UTR 35.0%
ACCAGTTGACAAAATGAAAG+GGG - chr7_2:67636605-67636624 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG - chr7_2:67636607-67636626 None:intergenic 35.0%
TTTCAAATCATCACATGGGT+AGG + chr7_2:67635430-67635449 Msa1071170:five_prime_UTR 35.0%
TTCAAATCATCACATGGGTA+GGG + chr7_2:67635431-67635450 Msa1071170:five_prime_UTR 35.0%
TAGGGTTATCCAACGAAATT+AGG + chr7_2:67635449-67635468 Msa1071170:five_prime_UTR 35.0%
AGGGTTATCCAACGAAATTA+GGG + chr7_2:67635450-67635469 Msa1071170:five_prime_UTR 35.0%
! TTTGGAGAGTCTTTTTACGT+CGG - chr7_2:67635597-67635616 None:intergenic 35.0%
! TTATCAGATGTTTGGGTTTG+AGG + chr7_2:67635795-67635814 Msa1071170:intron 35.0%
TTGTATGAGATGGATAAGGA+AGG - chr7_2:67635881-67635900 None:intergenic 35.0%
TACGTTGTATGAGATGGATA+AGG - chr7_2:67635885-67635904 None:intergenic 35.0%
TTAACAGGAATGGATGATGA+TGG - chr7_2:67635922-67635941 None:intergenic 35.0%
AAAGGAATGAAGATTGGAGA+TGG - chr7_2:67635986-67636005 None:intergenic 35.0%
GTTGTGAAAGGAATGAAGAT+TGG - chr7_2:67635992-67636011 None:intergenic 35.0%
!!! GAGACTTTTGAGACTACTAA+TGG + chr7_2:67636129-67636148 Msa1071170:CDS 35.0%
TACGTGATCATCTCATCTTA+GGG - chr7_2:67636269-67636288 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG - chr7_2:67636422-67636441 None:intergenic 35.0%
CACTACTTGTGATTTCTTGT+CGG + chr7_2:67636460-67636479 Msa1071170:three_prime_UTR 35.0%
ACTACTTGTGATTTCTTGTC+GGG + chr7_2:67636461-67636480 Msa1071170:three_prime_UTR 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG + chr7_2:67636476-67636495 Msa1071170:three_prime_UTR 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG + chr7_2:67636477-67636496 Msa1071170:three_prime_UTR 35.0%
ACCAGTTGACAAAATGAAAG+GGG - chr7_2:67636605-67636624 None:intergenic 35.0%
CTACCAGTTGACAAAATGAA+AGG - chr7_2:67636607-67636626 None:intergenic 35.0%
ATCTAGCACCCTAATTTCGT+TGG - chr7_2:67635461-67635480 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG - chr7_2:67635615-67635634 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG - chr7_2:67635648-67635667 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG - chr7_2:67635746-67635765 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG - chr7_2:67635831-67635850 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG - chr7_2:67635832-67635851 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG - chr7_2:67635867-67635886 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG - chr7_2:67635871-67635890 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG - chr7_2:67635937-67635956 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG + chr7_2:67635999-67636018 Msa1071170:CDS 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG - chr7_2:67636004-67636023 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG + chr7_2:67636165-67636184 Msa1071170:CDS 40.0%
CATCTTAGGGCCAATAAACA+AGG - chr7_2:67636256-67636275 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG - chr7_2:67636270-67636289 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG + chr7_2:67636297-67636316 Msa1071170:intron 40.0%
TGTTTAGAAGGGTCGACTAT+AGG + chr7_2:67636488-67636507 Msa1071170:three_prime_UTR 40.0%
GCAAGCAGACTTACAACAAA+AGG + chr7_2:67636516-67636535 Msa1071170:three_prime_UTR 40.0%
CTGGTAGCATTGTTTCCATT+AGG + chr7_2:67636620-67636639 Msa1071170:three_prime_UTR 40.0%
GTAGCATTGTTTCCATTAGG+AGG + chr7_2:67636623-67636642 Msa1071170:three_prime_UTR 40.0%
AACGTGAACAAACCTCCTAA+TGG - chr7_2:67636638-67636657 None:intergenic 40.0%
ATCTAGCACCCTAATTTCGT+TGG - chr7_2:67635461-67635480 None:intergenic 40.0%
ACTTACCTCTCGGTAGAATT+TGG - chr7_2:67635615-67635634 None:intergenic 40.0%
!! CTAGATGAGTGTCACTTCAT+AGG - chr7_2:67635648-67635667 None:intergenic 40.0%
AGGAACACATACACGAACAT+CGG - chr7_2:67635746-67635765 None:intergenic 40.0%
TCTTGAACTGTCCAAAGTCA+GGG - chr7_2:67635831-67635850 None:intergenic 40.0%
ATCTTGAACTGTCCAAAGTC+AGG - chr7_2:67635832-67635851 None:intergenic 40.0%
TAAGGAAGGATGAAGAAGGA+AGG - chr7_2:67635867-67635886 None:intergenic 40.0%
TGGATAAGGAAGGATGAAGA+AGG - chr7_2:67635871-67635890 None:intergenic 40.0%
ATATGAGAGGGGTCTTTAAC+AGG - chr7_2:67635937-67635956 None:intergenic 40.0%
TTCCTTTCACAACACTCTCA+AGG + chr7_2:67635999-67636018 Msa1071170:CDS 40.0%
!! AACCTTGAGAGTGTTGTGAA+AGG - chr7_2:67636004-67636023 None:intergenic 40.0%
! GTTTTCTTCTATGACCCTGA+TGG + chr7_2:67636165-67636184 Msa1071170:CDS 40.0%
CATCTTAGGGCCAATAAACA+AGG - chr7_2:67636256-67636275 None:intergenic 40.0%
CTACGTGATCATCTCATCTT+AGG - chr7_2:67636270-67636289 None:intergenic 40.0%
! TGTATATGCAGGTAATGGCT+TGG + chr7_2:67636297-67636316 Msa1071170:intron 40.0%
TGTTTAGAAGGGTCGACTAT+AGG + chr7_2:67636488-67636507 Msa1071170:three_prime_UTR 40.0%
GCAAGCAGACTTACAACAAA+AGG + chr7_2:67636516-67636535 Msa1071170:three_prime_UTR 40.0%
CTGGTAGCATTGTTTCCATT+AGG + chr7_2:67636620-67636639 Msa1071170:three_prime_UTR 40.0%
GTAGCATTGTTTCCATTAGG+AGG + chr7_2:67636623-67636642 Msa1071170:three_prime_UTR 40.0%
AACGTGAACAAACCTCCTAA+TGG - chr7_2:67636638-67636657 None:intergenic 40.0%
ACTCTCCAAATTCTACCGAG+AGG + chr7_2:67635607-67635626 Msa1071170:CDS 45.0%
CAACACAACACTGACACATG+CGG + chr7_2:67635676-67635695 Msa1071170:intron 45.0%
CTTGAACTGTCCAAAGTCAG+GGG - chr7_2:67635830-67635849 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG - chr7_2:67635891-67635910 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG - chr7_2:67635932-67635951 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG - chr7_2:67635969-67635988 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG + chr7_2:67636300-67636319 Msa1071170:intron 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG - chr7_2:67636514-67636533 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG + chr7_2:67636552-67636571 Msa1071170:three_prime_UTR 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG + chr7_2:67636601-67636620 Msa1071170:three_prime_UTR 45.0%
! GACGAGGTATCCATATTCCT+TGG - chr7_2:67636746-67636765 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG - chr7_2:67636762-67636781 None:intergenic 45.0%
ACTCTCCAAATTCTACCGAG+AGG + chr7_2:67635607-67635626 Msa1071170:CDS 45.0%
CAACACAACACTGACACATG+CGG + chr7_2:67635676-67635695 Msa1071170:intron 45.0%
CTTGAACTGTCCAAAGTCAG+GGG - chr7_2:67635830-67635849 None:intergenic 45.0%
CGTTGCTACGTTGTATGAGA+TGG - chr7_2:67635891-67635910 None:intergenic 45.0%
AGAGGGGTCTTTAACAGGAA+TGG - chr7_2:67635932-67635951 None:intergenic 45.0%
AGATGGAGAAGCAGAGATGA+TGG - chr7_2:67635969-67635988 None:intergenic 45.0%
! ATATGCAGGTAATGGCTTGG+AGG + chr7_2:67636300-67636319 Msa1071170:intron 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG - chr7_2:67636514-67636533 None:intergenic 45.0%
! TGTAGTTTGTGTGTTGCCTG+CGG + chr7_2:67636552-67636571 Msa1071170:three_prime_UTR 45.0%
! GCCCCTTTCATTTTGTCAAC+TGG + chr7_2:67636601-67636620 Msa1071170:three_prime_UTR 45.0%
! GACGAGGTATCCATATTCCT+TGG - chr7_2:67636746-67636765 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG - chr7_2:67636762-67636781 None:intergenic 45.0%
! GGAGAGTCTTTTTACGTCGG+TGG - chr7_2:67635594-67635613 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG - chr7_2:67635678-67635697 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG + chr7_2:67635817-67635836 Msa1071170:CDS 50.0%
CATACAACGTAGCAACGAGC+TGG + chr7_2:67635894-67635913 Msa1071170:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG - chr7_2:67635949-67635968 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG - chr7_2:67635950-67635969 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG - chr7_2:67635960-67635979 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG + chr7_2:67636557-67636576 Msa1071170:three_prime_UTR 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG + chr7_2:67636558-67636577 Msa1071170:three_prime_UTR 50.0%
AATAAAGCCACCCAAACCGC+AGG - chr7_2:67636571-67636590 None:intergenic 50.0%
! GGAGAGTCTTTTTACGTCGG+TGG - chr7_2:67635594-67635613 None:intergenic 50.0%
CGCATGTGTCAGTGTTGTGT+TGG - chr7_2:67635678-67635697 None:intergenic 50.0%
GAAGTTGAGACCCCTGACTT+TGG + chr7_2:67635817-67635836 Msa1071170:CDS 50.0%
CATACAACGTAGCAACGAGC+TGG + chr7_2:67635894-67635913 Msa1071170:CDS 50.0%
TGGCCTAAGCGGATATGAGA+GGG - chr7_2:67635949-67635968 None:intergenic 50.0%
ATGGCCTAAGCGGATATGAG+AGG - chr7_2:67635950-67635969 None:intergenic 50.0%
! AGCAGAGATGATGGCCTAAG+CGG - chr7_2:67635960-67635979 None:intergenic 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG + chr7_2:67636557-67636576 Msa1071170:three_prime_UTR 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG + chr7_2:67636558-67636577 Msa1071170:three_prime_UTR 50.0%
AATAAAGCCACCCAAACCGC+AGG - chr7_2:67636571-67636590 None:intergenic 50.0%
GAAGAAATGGCCGCAGCAGA+AGG + chr7_2:67635539-67635558 Msa1071170:exon 55.0%
CAGACTCACTCCTTCTGCTG+CGG - chr7_2:67635552-67635571 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG - chr7_2:67635577-67635596 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG + chr7_2:67635943-67635962 Msa1071170:CDS 55.0%
GGCCTAAGCGGATATGAGAG+GGG - chr7_2:67635948-67635967 None:intergenic 55.0%
GAAGAAATGGCCGCAGCAGA+AGG + chr7_2:67635539-67635558 Msa1071170:exon 55.0%
CAGACTCACTCCTTCTGCTG+CGG - chr7_2:67635552-67635571 None:intergenic 55.0%
! CGGTGGACTCTCGAGCTATA+TGG - chr7_2:67635577-67635596 None:intergenic 55.0%
GACCCCTCTCATATCCGCTT+AGG + chr7_2:67635943-67635962 Msa1071170:CDS 55.0%
GGCCTAAGCGGATATGAGAG+GGG - chr7_2:67635948-67635967 None:intergenic 55.0%
! TGTGTTGCCTGCGGTTTGGG+TGG + chr7_2:67636561-67636580 Msa1071170:three_prime_UTR 60.0%
! TGTGTTGCCTGCGGTTTGGG+TGG + chr7_2:67636561-67636580 Msa1071170:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 67635411 67636828 67635411 ID=Msa1071170;Name=Msa1071170
chr7_2 mRNA 67635411 67636828 67635411 ID=Msa1071170-mRNA-1;Parent=Msa1071170;Name=Msa1071170-mRNA-1;_AED=0.26;_eAED=0.26;_QI=134|1|1|1|1|1|4|477|138
chr7_2 exon 67635411 67635628 67635411 ID=Msa1071170-mRNA-1:exon:14653;Parent=Msa1071170-mRNA-1
chr7_2 exon 67635802 67636020 67635802 ID=Msa1071170-mRNA-1:exon:14654;Parent=Msa1071170-mRNA-1
chr7_2 exon 67636117 67636186 67636117 ID=Msa1071170-mRNA-1:exon:14655;Parent=Msa1071170-mRNA-1
chr7_2 exon 67636308 67636828 67636308 ID=Msa1071170-mRNA-1:exon:14656;Parent=Msa1071170-mRNA-1
chr7_2 five_prime_UTR 67635411 67635544 67635411 ID=Msa1071170-mRNA-1:five_prime_utr;Parent=Msa1071170-mRNA-1
chr7_2 CDS 67635545 67635628 67635545 ID=Msa1071170-mRNA-1:cds;Parent=Msa1071170-mRNA-1
chr7_2 CDS 67635802 67636020 67635802 ID=Msa1071170-mRNA-1:cds;Parent=Msa1071170-mRNA-1
chr7_2 CDS 67636117 67636186 67636117 ID=Msa1071170-mRNA-1:cds;Parent=Msa1071170-mRNA-1
chr7_2 CDS 67636308 67636351 67636308 ID=Msa1071170-mRNA-1:cds;Parent=Msa1071170-mRNA-1
chr7_2 three_prime_UTR 67636352 67636828 67636352 ID=Msa1071170-mRNA-1:three_prime_utr;Parent=Msa1071170-mRNA-1
Gene Sequence

>Msa1071170

ATGGCCGCAGCAGAAGGAGTGAGTCTGAACCATATAGCTCGAGAGTCCACCGACGTAAAAAGACTCTCCAAATTCTACCGAGAGATGTTTGGGTTTGAGGAAGTTGAGACCCCTGACTTTGGACAGTTCAAGATTATATGGTTGCGCCTTCCTTCTTCATCCTTCCTTATCCATCTCATACAACGTAGCAACGAGCTGGCTCCATCATCATCCATTCCTGTTAAAGACCCCTCTCATATCCGCTTAGGCCATCATCTCTGCTTCTCCATCTCCAATCTTCATTCCTTTCACAACACTCTCAAGGACAAAGGTATTGAGACTTTTGAGACTACTAATGGCAACATCAAACGAGTTTTCTTCTATGACCCTGATGGTAATGGCTTGGAGGTATTTGCTAGCATCGAAGACTCCTCCTAG
Protein sequence

>Msa1071170

MAAAEGVSLNHIARESTDVKRLSKFYREMFGFEEVETPDFGQFKIIWLRLPSSSFLIHLIQRSNELAPSSSIPVKDPSHIRLGHHLCFSISNLHSFHNTLKDKGIETFETTNGNIKRVFFYDPDGNGLEVFASIEDSS*