Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0621800 | Msa0795670 | 0.873010 | 2.047284e-67 | -8.615850e-47 |
| Msa0795670 | Msa0982510 | 0.829349 | 5.409541e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | 0.270466 | 5_3:-7706736 | None:intergenic |
| TGATGCTGCTGAAACTTCTC+TGG | 0.426565 | 5_3:-7706811 | None:intergenic |
| GGGAGAAACACAAAAGTAGA+AGG | 0.433808 | 5_3:+7706745 | Msa0795670:CDS |
| AAGGAAGAAGACAAAGGATG+AGG | 0.516251 | 5_3:+7706764 | Msa0795670:CDS |
| TGACTGAGGAAGCCCTAAAG+AGG | 0.599684 | 5_3:+7706724 | Msa0795670:CDS |
| GACTGAGGAAGCCCTAAAGA+GGG | 0.617145 | 5_3:+7706725 | Msa0795670:CDS |
| AAGTAGAAGGAAGAAGACAA+AGG | 0.649388 | 5_3:+7706758 | Msa0795670:CDS |
| GACTTACACTGATGTGACTG+AGG | 0.669624 | 5_3:+7706710 | Msa0795670:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | - | chr5_3:7706739-7706758 | None:intergenic | 35.0% | |
| ! | TTTTGTGTTTCTCCCTCTTT+AGG | - | chr5_3:7706740-7706759 | None:intergenic | 35.0% |
| AAGTAGAAGGAAGAAGACAA+AGG | + | chr5_3:7706758-7706777 | Msa0795670:CDS | 35.0% | |
| GGGAGAAACACAAAAGTAGA+AGG | + | chr5_3:7706745-7706764 | Msa0795670:CDS | 40.0% | |
| AAGGAAGAAGACAAAGGATG+AGG | + | chr5_3:7706764-7706783 | Msa0795670:CDS | 40.0% | |
| GACTTACACTGATGTGACTG+AGG | + | chr5_3:7706710-7706729 | Msa0795670:CDS | 45.0% | |
| TGATGCTGCTGAAACTTCTC+TGG | - | chr5_3:7706814-7706833 | None:intergenic | 45.0% | |
| TGACTGAGGAAGCCCTAAAG+AGG | + | chr5_3:7706724-7706743 | Msa0795670:CDS | 50.0% | |
| GACTGAGGAAGCCCTAAAGA+GGG | + | chr5_3:7706725-7706744 | Msa0795670:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 7706705 | 7706848 | 7706705 | ID=Msa0795670;Name=Msa0795670 |
| chr5_3 | mRNA | 7706705 | 7706848 | 7706705 | ID=Msa0795670-mRNA-1;Parent=Msa0795670;Name=Msa0795670-mRNA-1;_AED=0.49;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|47 |
| chr5_3 | exon | 7706705 | 7706848 | 7706705 | ID=Msa0795670-mRNA-1:exon:3180;Parent=Msa0795670-mRNA-1 |
| chr5_3 | CDS | 7706705 | 7706848 | 7706705 | ID=Msa0795670-mRNA-1:cds;Parent=Msa0795670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
