Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0040450 | Msa0982510 | 0.808335 | 3.257316e-50 | -8.615850e-47 |
| Msa0621800 | Msa0982510 | 0.934720 | 2.605904e-96 | -8.615850e-47 |
| Msa0693380 | Msa0982510 | 0.815098 | 1.100297e-51 | -8.615850e-47 |
| Msa0737570 | Msa0982510 | 0.800029 | 1.745445e-48 | -8.615850e-47 |
| Msa0795670 | Msa0982510 | 0.829349 | 5.409541e-55 | -8.615850e-47 |
| Msa0829620 | Msa0982510 | 0.815543 | 8.760965e-52 | -8.615850e-47 |
| Msa0191250 | Msa0982510 | 0.810748 | 9.879527e-51 | -8.615850e-47 |
| Msa0369850 | Msa0982510 | 0.804573 | 2.025016e-49 | -8.615850e-47 |
| Msa0982510 | Msa1082500 | 0.817001 | 4.134136e-52 | -8.615850e-47 |
| Msa0982510 | Msa1163540 | 0.811579 | 6.524559e-51 | -8.615850e-47 |
| Msa0982510 | Msa1340260 | 0.818948 | 1.501727e-52 | -8.615850e-47 |
| Msa0982510 | Msa1343070 | 0.802818 | 4.682821e-49 | -8.615850e-47 |
| Msa0357020 | Msa0982510 | 0.813636 | 2.315186e-51 | -8.615850e-47 |
| Msa0484780 | Msa0982510 | 0.807421 | 5.095617e-50 | -8.615850e-47 |
| Msa0503060 | Msa0982510 | 0.818097 | 2.340996e-52 | -8.615850e-47 |
| Msa0856920 | Msa0982510 | 0.807216 | 5.633370e-50 | -8.615850e-47 |
| Msa0867890 | Msa0982510 | 0.815463 | 9.125729e-52 | -8.615850e-47 |
| Msa0900620 | Msa0982510 | 0.801322 | 9.510285e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | 0.270466 | 6_4:-22200399 | None:intergenic |
| TGATGCTGCTGAAACTTCTC+TGG | 0.426565 | 6_4:-22200474 | None:intergenic |
| GGGAGAAACACAAAAGTAGA+AGG | 0.433808 | 6_4:+22200408 | Msa0982510:CDS |
| AAGGAAGAAGACAAAGGATG+AGG | 0.516251 | 6_4:+22200427 | Msa0982510:CDS |
| TGACTGAGGAAGCCCTAAAG+AGG | 0.599684 | 6_4:+22200387 | Msa0982510:CDS |
| GACTGAGGAAGCCCTAAAGA+GGG | 0.617145 | 6_4:+22200388 | Msa0982510:CDS |
| AAGTAGAAGGAAGAAGACAA+AGG | 0.649388 | 6_4:+22200421 | Msa0982510:CDS |
| GACTTACACTGATGTGACTG+AGG | 0.669624 | 6_4:+22200373 | Msa0982510:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | - | chr6_4:22200402-22200421 | None:intergenic | 35.0% | |
| ! | TTTTGTGTTTCTCCCTCTTT+AGG | - | chr6_4:22200403-22200422 | None:intergenic | 35.0% |
| AAGTAGAAGGAAGAAGACAA+AGG | + | chr6_4:22200421-22200440 | Msa0982510:CDS | 35.0% | |
| TTTGTGTTTCTCCCTCTTTA+GGG | - | chr6_4:22200402-22200421 | None:intergenic | 35.0% | |
| ! | TTTTGTGTTTCTCCCTCTTT+AGG | - | chr6_4:22200403-22200422 | None:intergenic | 35.0% |
| AAGTAGAAGGAAGAAGACAA+AGG | + | chr6_4:22200421-22200440 | Msa0982510:CDS | 35.0% | |
| GGGAGAAACACAAAAGTAGA+AGG | + | chr6_4:22200408-22200427 | Msa0982510:CDS | 40.0% | |
| AAGGAAGAAGACAAAGGATG+AGG | + | chr6_4:22200427-22200446 | Msa0982510:CDS | 40.0% | |
| GGGAGAAACACAAAAGTAGA+AGG | + | chr6_4:22200408-22200427 | Msa0982510:CDS | 40.0% | |
| AAGGAAGAAGACAAAGGATG+AGG | + | chr6_4:22200427-22200446 | Msa0982510:CDS | 40.0% | |
| GACTTACACTGATGTGACTG+AGG | + | chr6_4:22200373-22200392 | Msa0982510:CDS | 45.0% | |
| TGATGCTGCTGAAACTTCTC+TGG | - | chr6_4:22200477-22200496 | None:intergenic | 45.0% | |
| GACTTACACTGATGTGACTG+AGG | + | chr6_4:22200373-22200392 | Msa0982510:CDS | 45.0% | |
| TGATGCTGCTGAAACTTCTC+TGG | - | chr6_4:22200477-22200496 | None:intergenic | 45.0% | |
| TGACTGAGGAAGCCCTAAAG+AGG | + | chr6_4:22200387-22200406 | Msa0982510:CDS | 50.0% | |
| GACTGAGGAAGCCCTAAAGA+GGG | + | chr6_4:22200388-22200407 | Msa0982510:CDS | 50.0% | |
| TGACTGAGGAAGCCCTAAAG+AGG | + | chr6_4:22200387-22200406 | Msa0982510:CDS | 50.0% | |
| GACTGAGGAAGCCCTAAAGA+GGG | + | chr6_4:22200388-22200407 | Msa0982510:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 22200368 | 22200511 | 22200368 | ID=Msa0982510;Name=Msa0982510 |
| chr6_4 | mRNA | 22200368 | 22200511 | 22200368 | ID=Msa0982510-mRNA-1;Parent=Msa0982510;Name=Msa0982510-mRNA-1;_AED=0.49;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|47 |
| chr6_4 | exon | 22200368 | 22200511 | 22200368 | ID=Msa0982510-mRNA-1:exon:6991;Parent=Msa0982510-mRNA-1 |
| chr6_4 | CDS | 22200368 | 22200511 | 22200368 | ID=Msa0982510-mRNA-1:cds;Parent=Msa0982510-mRNA-1 |
| Gene Sequence |
| Protein sequence |
