Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0621800 | Msa0693380 | 0.801411 | 9.120451e-49 | -8.615850e-47 |
| Msa0621800 | Msa0795670 | 0.873010 | 2.047284e-67 | -8.615850e-47 |
| Msa0621800 | Msa0982510 | 0.934720 | 2.605904e-96 | -8.615850e-47 |
| Msa0621800 | Msa1163540 | 0.811779 | 5.901385e-51 | -8.615850e-47 |
| Msa0621800 | Msa1340260 | 0.815644 | 8.317557e-52 | -8.615850e-47 |
| Msa0503060 | Msa0621800 | 0.802831 | 4.655245e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | 0.270466 | 4_2:-74095925 | None:intergenic |
| TGATGCTGCTGAAACTTCTC+TGG | 0.426565 | 4_2:-74096000 | None:intergenic |
| GGGAGAAACACAAAAGTAGA+AGG | 0.433808 | 4_2:+74095934 | Msa0621800:CDS |
| AAGGAAGAAGACAAAGGATG+AGG | 0.516251 | 4_2:+74095953 | Msa0621800:CDS |
| TGACTGAGGAAGCCCTAAAG+AGG | 0.599684 | 4_2:+74095913 | Msa0621800:CDS |
| GACTGAGGAAGCCCTAAAGA+GGG | 0.617145 | 4_2:+74095914 | Msa0621800:CDS |
| AAGTAGAAGGAAGAAGACAA+AGG | 0.649388 | 4_2:+74095947 | Msa0621800:CDS |
| GACTTACACTGATGTGACTG+AGG | 0.669624 | 4_2:+74095899 | Msa0621800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTGTGTTTCTCCCTCTTTA+GGG | - | chr4_2:74095928-74095947 | None:intergenic | 35.0% | |
| ! | TTTTGTGTTTCTCCCTCTTT+AGG | - | chr4_2:74095929-74095948 | None:intergenic | 35.0% |
| AAGTAGAAGGAAGAAGACAA+AGG | + | chr4_2:74095947-74095966 | Msa0621800:CDS | 35.0% | |
| GGGAGAAACACAAAAGTAGA+AGG | + | chr4_2:74095934-74095953 | Msa0621800:CDS | 40.0% | |
| AAGGAAGAAGACAAAGGATG+AGG | + | chr4_2:74095953-74095972 | Msa0621800:CDS | 40.0% | |
| GACTTACACTGATGTGACTG+AGG | + | chr4_2:74095899-74095918 | Msa0621800:CDS | 45.0% | |
| TGATGCTGCTGAAACTTCTC+TGG | - | chr4_2:74096003-74096022 | None:intergenic | 45.0% | |
| TGACTGAGGAAGCCCTAAAG+AGG | + | chr4_2:74095913-74095932 | Msa0621800:CDS | 50.0% | |
| GACTGAGGAAGCCCTAAAGA+GGG | + | chr4_2:74095914-74095933 | Msa0621800:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 74095894 | 74096037 | 74095894 | ID=Msa0621800;Name=Msa0621800 |
| chr4_2 | mRNA | 74095894 | 74096037 | 74095894 | ID=Msa0621800-mRNA-1;Parent=Msa0621800;Name=Msa0621800-mRNA-1;_AED=0.49;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|47 |
| chr4_2 | exon | 74095894 | 74096037 | 74095894 | ID=Msa0621800-mRNA-1:exon:22320;Parent=Msa0621800-mRNA-1 |
| chr4_2 | CDS | 74095894 | 74096037 | 74095894 | ID=Msa0621800-mRNA-1:cds;Parent=Msa0621800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
