AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0802680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0802680 MtrunA17_Chr5g0410531 79.191 173 11 1 1 148 1 173 5.15e-91 270
Msa0802680 MtrunA17_Chr8g0369161 66.857 175 31 3 1 148 1 175 2.24e-69 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0802680 AT1G32150.1 58.152 184 41 4 1 148 1 184 1.31e-60 192
Msa0802680 AT2G35530.1 60.000 180 40 3 1 148 1 180 1.16e-57 184

Find 80 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGACTGGGCCAGCTTTC+AGG 0.108461 5_3:-17241883 Msa0802680:intron
ATTACCACTCAAAGAGAAAT+TGG 0.187816 5_3:+17242633 None:intergenic
GCGTAGGAAGAAGGTGAATT+TGG 0.230889 5_3:+17242738 None:intergenic
TTCACTGATATATTAAAGTA+TGG 0.280019 5_3:-17242095 Msa0802680:exon
CAGCACATGATGCCTCCTTA+TGG 0.341092 5_3:-17240721 Msa0802680:CDS
GGTAGTTTGAACATGATTAC+AGG 0.349225 5_3:-17240265 Msa0802680:CDS
ACAGTAGGAGGGGTCAAAAT+TGG 0.356551 5_3:+17242696 None:intergenic
GGATCCTTCAGAGCTTGATT+TGG 0.372607 5_3:+17242656 None:intergenic
CGTAGGAAGAAGGTGAATTT+GGG 0.375751 5_3:+17242739 None:intergenic
AAAAGGTCAAAAGGAAGTTT+AGG 0.381702 5_3:-17240286 Msa0802680:CDS
CACCAAAATTTACCTCTTTG+AGG 0.387581 5_3:+17240192 None:intergenic
AATAGGAACGAAAATCTAAC+TGG 0.388651 5_3:-17242932 Msa0802680:five_prime_UTR
GTAGGCATGGCATAAGGATA+TGG 0.411726 5_3:+17240522 None:intergenic
TTACCACTCAAAGAGAAATT+GGG 0.415228 5_3:+17242634 None:intergenic
ACCAAAATTTACCTCTTTGA+GGG 0.430420 5_3:+17240193 None:intergenic
CCTTCGACCGCCACTAGCAC+TGG 0.444211 5_3:-17241915 Msa0802680:CDS
ACCTGAAAGCTGGCCCAGTC+AGG 0.449030 5_3:+17241882 None:intergenic
TAAGATTGAATTGATGAGAA+GGG 0.463946 5_3:+17242806 None:intergenic
TTACCAATCAAAAGGTCAAA+AGG 0.467960 5_3:-17240295 Msa0802680:CDS
CCATCAGTTTCCATGCTGGC+AGG 0.471564 5_3:+17240343 None:intergenic
ACAGCGGGGACATCTGCTAA+TGG 0.473912 5_3:-17240220 Msa0802680:CDS
ATGGGGATACATTGCAACAT+AGG 0.481817 5_3:+17240684 None:intergenic
GAGGCCTCAGCAATACCGTT+TGG 0.488036 5_3:+17240498 None:intergenic
AGCAGTGAATATACAGTAGG+AGG 0.491527 5_3:+17242684 None:intergenic
CTGGAACAGTTAATCCTGAC+TGG 0.502822 5_3:-17241896 Msa0802680:CDS
CTCTCCAAATCAAGCTCTGA+AGG 0.511423 5_3:-17242660 Msa0802680:five_prime_UTR
AATGAACATGGTAAAACAGC+GGG 0.512685 5_3:-17240235 Msa0802680:CDS
TGCTCATCCATCCATGCCTC+CGG 0.520704 5_3:-17240650 Msa0802680:intron
ATGATTACCGGAGGCATGGA+TGG 0.528642 5_3:+17240643 None:intergenic
GGAACTCGTGGGTCGTCGTA+CGG 0.531318 5_3:+17242896 None:intergenic
TGGGGATACATTGCAACATA+GGG 0.533477 5_3:+17240685 None:intergenic
AGTAGCAGTGAATATACAGT+AGG 0.533650 5_3:+17242681 None:intergenic
TTTGGAGTAGGCATGGCATA+AGG 0.540351 5_3:+17240516 None:intergenic
GGAAATTGAGCGTAGGAAGA+AGG 0.545805 5_3:+17242729 None:intergenic
GGATGTGGTGGAGTACCATA+AGG 0.547567 5_3:+17240706 None:intergenic
GACAATGATTACCGGAGGCA+TGG 0.549201 5_3:+17240639 None:intergenic
TTTACCATCAGTTTCCATGC+TGG 0.549293 5_3:+17240339 None:intergenic
ATCCCCAATTTCTCTTTGAG+TGG 0.549840 5_3:-17242637 Msa0802680:intron
GGGAAACACTCCTGCCAGCA+TGG 0.550438 5_3:-17240353 Msa0802680:CDS
TATGCCATGCCTACTCCAAA+CGG 0.550779 5_3:-17240513 Msa0802680:CDS
CTCAATTAGACCTGTGATGT+TGG 0.552139 5_3:+17242019 None:intergenic
ACTCGTGGGTCGTCGTACGG+TGG 0.552979 5_3:+17242899 None:intergenic
TTAAGATTGAATTGATGAGA+AGG 0.554419 5_3:+17242805 None:intergenic
GTTAATGTGTTTGTTGTTCG+CGG 0.554514 5_3:+17242870 None:intergenic
TCACTGATATATTAAAGTAT+GGG 0.555082 5_3:-17242094 Msa0802680:exon
GCAGTGAATATACAGTAGGA+GGG 0.561436 5_3:+17242685 None:intergenic
CCAGTGCTAGTGGCGGTCGA+AGG 0.566554 5_3:+17241915 None:intergenic
GACACCACCACCAACATCAC+AGG 0.567614 5_3:-17242029 Msa0802680:intron
CTCGTGGGTCGTCGTACGGT+GGG 0.572804 5_3:+17242900 None:intergenic
AAGATTGAATTGATGAGAAG+GGG 0.573809 5_3:+17242807 None:intergenic
TGGTAAACCTCCTGAAGTGA+AGG 0.575107 5_3:-17240323 Msa0802680:CDS
AAGTATGGGCAATAATGAGA+TGG 0.579789 5_3:-17242080 Msa0802680:exon
TACATTGCAACATAGGGATG+TGG 0.580807 5_3:+17240691 None:intergenic
TAGACCTGTGATGTTGGTGG+TGG 0.584496 5_3:+17242025 None:intergenic
GGATAAAACAGCAAAAGAGA+AGG 0.588848 5_3:-17242059 Msa0802680:CDS
ATTAACTGTTCCAGTGCTAG+TGG 0.593825 5_3:+17241905 None:intergenic
TACCACTCAAAGAGAAATTG+GGG 0.600205 5_3:+17242635 None:intergenic
TCAGCAATACCGTTTGGAGT+AGG 0.605905 5_3:+17240504 None:intergenic
GATGAGCATATATGCCACCA+TGG 0.610974 5_3:+17240665 None:intergenic
ATTGCAACATAGGGATGTGG+TGG 0.613657 5_3:+17240694 None:intergenic
AACTGTTCCAGTGCTAGTGG+CGG 0.616899 5_3:+17241908 None:intergenic
TGTGTTTGTTGTTCGCGGAG+AGG 0.619030 5_3:+17242875 None:intergenic
AGATTGAATTGATGAGAAGG+GGG 0.621107 5_3:+17242808 None:intergenic
GTAGTTTGAACATGATTACA+GGG 0.624590 5_3:-17240264 Msa0802680:CDS
CCTGCCAGCATGGAAACTGA+TGG 0.625964 5_3:-17240343 Msa0802680:CDS
TGGAACAGTTAATCCTGACT+GGG 0.626947 5_3:-17241895 Msa0802680:CDS
ATGAGCATATATGCCACCAT+GGG 0.627162 5_3:+17240666 None:intergenic
CTAATGGAATTCCCTCAAAG+AGG 0.629685 5_3:-17240204 None:intergenic
TACTCCAAACGGTATTGCTG+AGG 0.632684 5_3:-17240502 Msa0802680:CDS
AATACCGTTTGGAGTAGGCA+TGG 0.637188 5_3:+17240509 None:intergenic
TGTTCGCGGAGAGGAACTCG+TGG 0.639993 5_3:+17242884 None:intergenic
AATTAGACCTGTGATGTTGG+TGG 0.642546 5_3:+17242022 None:intergenic
GTTCGCGGAGAGGAACTCGT+GGG 0.656197 5_3:+17242885 None:intergenic
TATGTTGCAATGTATCCCCA+TGG 0.658872 5_3:-17240682 Msa0802680:CDS
CAGTGAATATACAGTAGGAG+GGG 0.682502 5_3:+17242686 None:intergenic
TGTGGTGGAGTACCATAAGG+AGG 0.685282 5_3:+17240709 None:intergenic
TAATGAACATGGTAAAACAG+CGG 0.688598 5_3:-17240236 Msa0802680:CDS
TGAGCATATATGCCACCATG+GGG 0.728314 5_3:+17240667 None:intergenic
GTTGCAATGTATCCCCATGG+TGG 0.737517 5_3:-17240679 Msa0802680:CDS
ATGAACATGGTAAAACAGCG+GGG 0.756524 5_3:-17240234 Msa0802680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAACTAGAATTTTTTT+TGG - chr5_3:17241276-17241295 Msa0802680:intron 10.0%
!! AAAATATCATAAATATAAAG+CGG - chr5_3:17241809-17241828 Msa0802680:intron 10.0%
!!! TAAAATCAAAATTGGATTTT+TGG - chr5_3:17240717-17240736 Msa0802680:CDS 15.0%
!!! TTTTGTTTTATCTATATTGA+TGG - chr5_3:17241144-17241163 Msa0802680:intron 15.0%
!!! ACACTATTTTCTATTATTTA+CGG - chr5_3:17241300-17241319 Msa0802680:intron 15.0%
!!! TTGTAAATTGACTTATAATT+TGG - chr5_3:17241659-17241678 Msa0802680:intron 15.0%
!!! TGTAAATTGACTTATAATTT+GGG - chr5_3:17241660-17241679 Msa0802680:intron 15.0%
!!! TTTGTGAAAATTTTGATCTT+AGG + chr5_3:17240361-17240380 None:intergenic 20.0%
!!! TCAATTTCACTTTTTATCTT+TGG - chr5_3:17240613-17240632 Msa0802680:intron 20.0%
!!! ATAATTGAATTTGCTATGTT+GGG - chr5_3:17240675-17240694 Msa0802680:CDS 20.0%
!! ACATAGAGTAAAATCAAAAT+TGG - chr5_3:17240709-17240728 Msa0802680:CDS 20.0%
!! TTCACTGATATATTAAAGTA+TGG - chr5_3:17241041-17241060 Msa0802680:intron 20.0%
!! TCACTGATATATTAAAGTAT+GGG - chr5_3:17241042-17241061 Msa0802680:intron 20.0%
!!! TTATCTATATTGATGGTTAA+TGG - chr5_3:17241151-17241170 Msa0802680:intron 20.0%
!!! GAAACATAGTTAATTTTGTA+TGG - chr5_3:17241501-17241520 Msa0802680:intron 20.0%
!! GCTAAATTAAAAGAATTAGA+AGG - chr5_3:17241581-17241600 Msa0802680:intron 20.0%
!!! AAGGAGTATTAAATAGTAAA+GGG - chr5_3:17241600-17241619 Msa0802680:intron 20.0%
!! CTTTATTGAAATTGTCTATT+TGG + chr5_3:17241789-17241808 None:intergenic 20.0%
!! ACATATCAATTAAAATTTCC+CGG - chr5_3:17242003-17242022 Msa0802680:intron 20.0%
!!! AAAATGAATAATTCAGGTTT+TGG - chr5_3:17242233-17242252 Msa0802680:intron 20.0%
!! TAAATGAACCAAATAAAACA+CGG + chr5_3:17242380-17242399 None:intergenic 20.0%
!! ATCAAAAATTAGGGTTTGTT+GGG + chr5_3:17240291-17240310 None:intergenic 25.0%
! TAAGATTGAATTGATGAGAA+GGG + chr5_3:17240333-17240352 None:intergenic 25.0%
! TTAAGATTGAATTGATGAGA+AGG + chr5_3:17240334-17240353 None:intergenic 25.0%
!!! CAGCTTTATTTGCTTTTAAT+TGG - chr5_3:17240539-17240558 Msa0802680:intron 25.0%
!!! GATAATTGAATTTGCTATGT+TGG - chr5_3:17240674-17240693 Msa0802680:CDS 25.0%
!! AGAATATGCCTATACAATTT+TGG + chr5_3:17241350-17241369 None:intergenic 25.0%
!!! GTTTCTTAATTTGTGTGAAA+TGG + chr5_3:17241486-17241505 None:intergenic 25.0%
!!! GAAGGAGTATTAAATAGTAA+AGG - chr5_3:17241599-17241618 Msa0802680:intron 25.0%
! AGTTACTTCCATTCATTATT+GGG + chr5_3:17242199-17242218 None:intergenic 25.0%
!! CTGAATTATTCATTTTGTAG+TGG + chr5_3:17242229-17242248 None:intergenic 25.0%
! CACTACAAAATGAATAATTC+AGG - chr5_3:17242227-17242246 Msa0802680:intron 25.0%
! AGAAGACTAATAAATAGTTG+AGG + chr5_3:17242260-17242279 None:intergenic 25.0%
! AAATGAACCAAATAAAACAC+GGG + chr5_3:17242379-17242398 None:intergenic 25.0%
! TCATAATTGACAATGATTAC+CGG + chr5_3:17242508-17242527 None:intergenic 25.0%
! CATTGTCAATTATGATTTCA+TGG - chr5_3:17242513-17242532 Msa0802680:intron 25.0%
!!! ACTTTTTCCCTACTTATATA+AGG + chr5_3:17242745-17242764 None:intergenic 25.0%
! AGGAAAAATTACCAATCAAA+AGG - chr5_3:17242833-17242852 Msa0802680:five_prime_UTR 25.0%
GATCAAAAATTAGGGTTTGT+TGG + chr5_3:17240292-17240311 None:intergenic 30.0%
TGGGGATAGATCAAAAATTA+GGG + chr5_3:17240300-17240319 None:intergenic 30.0%
TTGGGGATAGATCAAAAATT+AGG + chr5_3:17240301-17240320 None:intergenic 30.0%
AAGATTGAATTGATGAGAAG+GGG + chr5_3:17240332-17240351 None:intergenic 30.0%
GTCAAAATTGGCAAGAAAAA+TGG + chr5_3:17240431-17240450 None:intergenic 30.0%
TTACCACTCAAAGAGAAATT+GGG + chr5_3:17240505-17240524 None:intergenic 30.0%
ATTACCACTCAAAGAGAAAT+TGG + chr5_3:17240506-17240525 None:intergenic 30.0%
!!! TCATCTTGATTTTCTAGCTT+TGG - chr5_3:17240638-17240657 Msa0802680:intron 30.0%
! TGGATGATTTTGCAATGATT+AGG - chr5_3:17240737-17240756 Msa0802680:intron 30.0%
! ATGATTTTGCAATGATTAGG+AGG - chr5_3:17240740-17240759 Msa0802680:intron 30.0%
! TGATTTTGCAATGATTAGGA+GGG - chr5_3:17240741-17240760 Msa0802680:intron 30.0%
CATAGTTAACTTTACAGTTG+TGG - chr5_3:17240905-17240924 Msa0802680:intron 30.0%
AACTTTACAGTTGTGGAATT+CGG - chr5_3:17240912-17240931 Msa0802680:intron 30.0%
ATTTCGAATGAGAATGAGTA+TGG - chr5_3:17240948-17240967 Msa0802680:intron 30.0%
! GTTATGTGTATTTTGCAGAA+TGG - chr5_3:17240998-17241017 Msa0802680:intron 30.0%
!! GAAGCATTTATTGTTTTTCC+AGG - chr5_3:17241193-17241212 Msa0802680:intron 30.0%
! TGACTTATAATTTGTGACAG+AGG + chr5_3:17241457-17241476 None:intergenic 30.0%
ATTATCCTTCATTAACTACG+TGG - chr5_3:17241554-17241573 Msa0802680:intron 30.0%
TTATCCTTCATTAACTACGT+GGG - chr5_3:17241555-17241574 Msa0802680:intron 30.0%
! TAAATAGTAAAGGGTGTGAT+AGG - chr5_3:17241609-17241628 Msa0802680:intron 30.0%
GATATGTATAATTGCATGCA+TGG + chr5_3:17241990-17242009 None:intergenic 30.0%
ATTGTATTCCTTAAGCTCTA+AGG + chr5_3:17242112-17242131 None:intergenic 30.0%
TTGAAACTCCCAATAATGAA+TGG - chr5_3:17242188-17242207 Msa0802680:intron 30.0%
GAGTTACTTCCATTCATTAT+TGG + chr5_3:17242200-17242219 None:intergenic 30.0%
! AACAAGATATTTTGTACTGC+TGG + chr5_3:17242346-17242365 None:intergenic 30.0%
AATGAACCAAATAAAACACG+GGG + chr5_3:17242378-17242397 None:intergenic 30.0%
TAATTGCGGAAAATTTACAC+AGG - chr5_3:17242583-17242602 Msa0802680:intron 30.0%
AATTGCGGAAAATTTACACA+GGG - chr5_3:17242584-17242603 Msa0802680:intron 30.0%
TTACCAATCAAAAGGTCAAA+AGG - chr5_3:17242841-17242860 Msa0802680:five_prime_UTR 30.0%
AAAAGGTCAAAAGGAAGTTT+AGG - chr5_3:17242850-17242869 Msa0802680:five_prime_UTR 30.0%
GTAGTTTGAACATGATTACA+GGG - chr5_3:17242872-17242891 Msa0802680:five_prime_UTR 30.0%
ACAGGGAAAAATAATGAACA+TGG - chr5_3:17242889-17242908 Msa0802680:five_prime_UTR 30.0%
TAATGAACATGGTAAAACAG+CGG - chr5_3:17242900-17242919 Msa0802680:five_prime_UTR 30.0%
!! GTTAATGTGTTTGTTGTTCG+CGG + chr5_3:17240269-17240288 None:intergenic 35.0%
AGATTGAATTGATGAGAAGG+GGG + chr5_3:17240331-17240350 None:intergenic 35.0%
GAAAAATGGAAATTGAGCGT+AGG + chr5_3:17240417-17240436 None:intergenic 35.0%
AGTAGCAGTGAATATACAGT+AGG + chr5_3:17240458-17240477 None:intergenic 35.0%
TACCACTCAAAGAGAAATTG+GGG + chr5_3:17240504-17240523 None:intergenic 35.0%
!! TGAATTTGCTATGTTGGGTT+TGG - chr5_3:17240680-17240699 Msa0802680:CDS 35.0%
! GATTTTGCAATGATTAGGAG+GGG - chr5_3:17240742-17240761 Msa0802680:intron 35.0%
GTTAATGGAATGGTTCATTG+CGG - chr5_3:17240782-17240801 Msa0802680:intron 35.0%
AAGTATGGGCAATAATGAGA+TGG - chr5_3:17241056-17241075 Msa0802680:intron 35.0%
GGATAAAACAGCAAAAGAGA+AGG - chr5_3:17241077-17241096 Msa0802680:intron 35.0%
CTAGTTAAAAACCTGAAAGC+TGG + chr5_3:17241267-17241286 None:intergenic 35.0%
TATCCTTCATTAACTACGTG+GGG - chr5_3:17241556-17241575 Msa0802680:intron 35.0%
! GACTTATAATTTGGGACTGA+TGG - chr5_3:17241668-17241687 Msa0802680:intron 35.0%
!! TTTGCAAGATTTTAGCGCAA+AGG + chr5_3:17241752-17241771 None:intergenic 35.0%
AAATTCTCTACATCCTTGGT+TGG + chr5_3:17241941-17241960 None:intergenic 35.0%
! TGCATGGAAATTTTTGCTCA+TGG + chr5_3:17241974-17241993 None:intergenic 35.0%
ATAAAGTCCTCTTCATACAC+CGG + chr5_3:17242025-17242044 None:intergenic 35.0%
!! CCTTAAGCTCTAAGGAATTA+GGG + chr5_3:17242104-17242123 None:intergenic 35.0%
!! TCCTTAAGCTCTAAGGAATT+AGG + chr5_3:17242105-17242124 None:intergenic 35.0%
AAACGTGTATGTCTTAACGA+AGG - chr5_3:17242159-17242178 Msa0802680:intron 35.0%
AACGTGTATGTCTTAACGAA+GGG - chr5_3:17242160-17242179 Msa0802680:intron 35.0%
TAATTGACAATGATTACCGG+AGG + chr5_3:17242505-17242524 None:intergenic 35.0%
!!! CAACCACTTTTTGCTAATTG+CGG - chr5_3:17242569-17242588 Msa0802680:intron 35.0%
TTTCCGCAATTAGCAAAAAG+TGG + chr5_3:17242575-17242594 None:intergenic 35.0%
AGGAACACAAAAATGCAGAA+GGG + chr5_3:17242677-17242696 None:intergenic 35.0%
AAGGAACACAAAAATGCAGA+AGG + chr5_3:17242678-17242697 None:intergenic 35.0%
AATAGGAAAAGGACAGACAA+AGG + chr5_3:17242707-17242726 None:intergenic 35.0%
! TCCTTTTCCTATTCTGTGAA+GGG - chr5_3:17242714-17242733 Msa0802680:five_prime_UTR 35.0%
GGCTTGTCCTTATATAAGTA+GGG - chr5_3:17242735-17242754 Msa0802680:five_prime_UTR 35.0%
! AATTTTTCCTTCACTTCAGG+AGG + chr5_3:17242823-17242842 None:intergenic 35.0%
! GGTAATTTTTCCTTCACTTC+AGG + chr5_3:17242826-17242845 None:intergenic 35.0%
! CTTCCTTTTGACCTTTTGAT+TGG + chr5_3:17242847-17242866 None:intergenic 35.0%
GGTAGTTTGAACATGATTAC+AGG - chr5_3:17242871-17242890 Msa0802680:five_prime_UTR 35.0%
AATGAACATGGTAAAACAGC+GGG - chr5_3:17242901-17242920 Msa0802680:five_prime_UTR 35.0%
CGTAGGAAGAAGGTGAATTT+GGG + chr5_3:17240400-17240419 None:intergenic 40.0%
CAGTGAATATACAGTAGGAG+GGG + chr5_3:17240453-17240472 None:intergenic 40.0%
GCAGTGAATATACAGTAGGA+GGG + chr5_3:17240454-17240473 None:intergenic 40.0%
AGCAGTGAATATACAGTAGG+AGG + chr5_3:17240455-17240474 None:intergenic 40.0%
ATCCCCAATTTCTCTTTGAG+TGG - chr5_3:17240499-17240518 Msa0802680:CDS 40.0%
CAATGATTAGGAGGGGAATT+AGG - chr5_3:17240749-17240768 Msa0802680:intron 40.0%
!! TTAGGATGGTGGGTTGTTAA+TGG - chr5_3:17240767-17240786 Msa0802680:intron 40.0%
!! ATGGTGGGTTGTTAATGGAA+TGG - chr5_3:17240772-17240791 Msa0802680:intron 40.0%
AATTAGACCTGTGATGTTGG+TGG + chr5_3:17241117-17241136 None:intergenic 40.0%
CTCAATTAGACCTGTGATGT+TGG + chr5_3:17241120-17241139 None:intergenic 40.0%
! TGGTACATTTTGTGTGACGA+AGG - chr5_3:17241171-17241190 Msa0802680:intron 40.0%
ATTAACTGTTCCAGTGCTAG+TGG + chr5_3:17241234-17241253 None:intergenic 40.0%
TGGAACAGTTAATCCTGACT+GGG - chr5_3:17241241-17241260 Msa0802680:intron 40.0%
ACCCTGCTCCAAAATTGTAT+AGG - chr5_3:17241339-17241358 Msa0802680:intron 40.0%
!!! GCCTATACAATTTTGGAGCA+GGG + chr5_3:17241343-17241362 None:intergenic 40.0%
!!! TGCCTATACAATTTTGGAGC+AGG + chr5_3:17241344-17241363 None:intergenic 40.0%
GTAGTATATGCGTCATTACC+TGG + chr5_3:17241435-17241454 None:intergenic 40.0%
! GCGGACTACATAAAACATAG+TGG - chr5_3:17241828-17241847 Msa0802680:intron 40.0%
ATCTCTGTGATGGTTCTTTC+TGG + chr5_3:17241872-17241891 None:intergenic 40.0%
AAGAATGCTCATCTCTGTGA+TGG + chr5_3:17241882-17241901 None:intergenic 40.0%
TCACAGAGATGAGCATTCTT+AGG - chr5_3:17241882-17241901 Msa0802680:intron 40.0%
GCATTCTTAGGTTTAAGAGC+TGG - chr5_3:17241894-17241913 Msa0802680:CDS 40.0%
GAGCTGGATAGAAATGTTGT+CGG - chr5_3:17241910-17241929 Msa0802680:CDS 40.0%
CAGCAAATTCTCTACATCCT+TGG + chr5_3:17241945-17241964 None:intergenic 40.0%
CAAGGATGTAGAGAATTTGC+TGG - chr5_3:17241943-17241962 Msa0802680:intron 40.0%
AAATTTCCCGGTGTATGAAG+AGG - chr5_3:17242015-17242034 Msa0802680:intron 40.0%
TAAAGTCCTCTTCATACACC+GGG + chr5_3:17242024-17242043 None:intergenic 40.0%
TTTATGCTCTCTCATGTGCT+AGG - chr5_3:17242040-17242059 Msa0802680:CDS 40.0%
CCCTAATTCCTTAGAGCTTA+AGG - chr5_3:17242101-17242120 Msa0802680:five_prime_UTR 40.0%
!! ACACTTCCCCGTGTTTTATT+TGG - chr5_3:17242369-17242388 Msa0802680:intron 40.0%
TACATTGCAACATAGGGATG+TGG + chr5_3:17242448-17242467 None:intergenic 40.0%
TGGGGATACATTGCAACATA+GGG + chr5_3:17242454-17242473 None:intergenic 40.0%
ATGGGGATACATTGCAACAT+AGG + chr5_3:17242455-17242474 None:intergenic 40.0%
TATGTTGCAATGTATCCCCA+TGG - chr5_3:17242454-17242473 Msa0802680:intron 40.0%
ATGAGCATATATGCCACCAT+GGG + chr5_3:17242473-17242492 None:intergenic 40.0%
!! GCAAAAAGTGGTTGGTGAAT+TGG + chr5_3:17242563-17242582 None:intergenic 40.0%
CGCAATTAGCAAAAAGTGGT+TGG + chr5_3:17242571-17242590 None:intergenic 40.0%
GAACACAAAAATGCAGAAGG+GGG + chr5_3:17242675-17242694 None:intergenic 40.0%
GGAACACAAAAATGCAGAAG+GGG + chr5_3:17242676-17242695 None:intergenic 40.0%
! GTCCTTTTCCTATTCTGTGA+AGG - chr5_3:17242713-17242732 Msa0802680:five_prime_UTR 40.0%
GGGCTTGTCCTTATATAAGT+AGG - chr5_3:17242734-17242753 Msa0802680:five_prime_UTR 40.0%
AGTAACACGAGTTTACATGC+AGG - chr5_3:17242762-17242781 Msa0802680:five_prime_UTR 40.0%
GTAACACGAGTTTACATGCA+GGG - chr5_3:17242763-17242782 Msa0802680:five_prime_UTR 40.0%
TTTACCATCAGTTTCCATGC+TGG + chr5_3:17242800-17242819 None:intergenic 40.0%
ATGAACATGGTAAAACAGCG+GGG - chr5_3:17242902-17242921 Msa0802680:five_prime_UTR 40.0%
TGAGAAGGGGGCAAAGAATT+GGG + chr5_3:17240319-17240338 None:intergenic 45.0%
ATGAGAAGGGGGCAAAGAAT+TGG + chr5_3:17240320-17240339 None:intergenic 45.0%
GCGTAGGAAGAAGGTGAATT+TGG + chr5_3:17240401-17240420 None:intergenic 45.0%
GGAAATTGAGCGTAGGAAGA+AGG + chr5_3:17240410-17240429 None:intergenic 45.0%
ACAGTAGGAGGGGTCAAAAT+TGG + chr5_3:17240443-17240462 None:intergenic 45.0%
! CTCTCCAAATCAAGCTCTGA+AGG - chr5_3:17240476-17240495 Msa0802680:intron 45.0%
GGATCCTTCAGAGCTTGATT+TGG + chr5_3:17240483-17240502 None:intergenic 45.0%
GATTAGGAGGGGAATTAGGA+TGG - chr5_3:17240753-17240772 Msa0802680:intron 45.0%
CTGGAACAGTTAATCCTGAC+TGG - chr5_3:17241240-17241259 Msa0802680:intron 45.0%
TATTCTCCTATGCCTCCACA+TGG - chr5_3:17241364-17241383 Msa0802680:intron 45.0%
! ATGAATCCATGTGGAGGCAT+AGG + chr5_3:17241373-17241392 None:intergenic 45.0%
TATGCCTCCACATGGATTCA+TGG - chr5_3:17241372-17241391 Msa0802680:intron 45.0%
CACATATAAGGGTGAGCTTG+GGG + chr5_3:17241406-17241425 None:intergenic 45.0%
CCACATATAAGGGTGAGCTT+GGG + chr5_3:17241407-17241426 None:intergenic 45.0%
CCAAGCTCACCCTTATATGT+GGG - chr5_3:17241405-17241424 Msa0802680:intron 45.0%
CAAGCTCACCCTTATATGTG+GGG - chr5_3:17241406-17241425 Msa0802680:intron 45.0%
CTTCCCCACGTAGTTAATGA+AGG + chr5_3:17241562-17241581 None:intergenic 45.0%
ATTGCAACATAGGGATGTGG+TGG + chr5_3:17242445-17242464 None:intergenic 45.0%
TGAGCATATATGCCACCATG+GGG + chr5_3:17242472-17242491 None:intergenic 45.0%
GATGAGCATATATGCCACCA+TGG + chr5_3:17242474-17242493 None:intergenic 45.0%
GTAGGCATGGCATAAGGATA+TGG + chr5_3:17242617-17242636 None:intergenic 45.0%
! TTTGGAGTAGGCATGGCATA+AGG + chr5_3:17242623-17242642 None:intergenic 45.0%
TATGCCATGCCTACTCCAAA+CGG - chr5_3:17242623-17242642 Msa0802680:intron 45.0%
! AATACCGTTTGGAGTAGGCA+TGG + chr5_3:17242630-17242649 None:intergenic 45.0%
! TCAGCAATACCGTTTGGAGT+AGG + chr5_3:17242635-17242654 None:intergenic 45.0%
TACTCCAAACGGTATTGCTG+AGG - chr5_3:17242634-17242653 Msa0802680:intron 45.0%
GGACAGACAAAGGAGAAAGA+AGG + chr5_3:17242697-17242716 None:intergenic 45.0%
GCCCTTCACAGAATAGGAAA+AGG + chr5_3:17242718-17242737 None:intergenic 45.0%
TGGTAAACCTCCTGAAGTGA+AGG - chr5_3:17242813-17242832 Msa0802680:five_prime_UTR 45.0%
!! TGTGTTTGTTGTTCGCGGAG+AGG + chr5_3:17240264-17240283 None:intergenic 50.0%
GAGAAGGGGGCAAAGAATTG+GGG + chr5_3:17240318-17240337 None:intergenic 50.0%
TAGGAGGGGAATTAGGATGG+TGG - chr5_3:17240756-17240775 Msa0802680:intron 50.0%
AGGAGGGGAATTAGGATGGT+GGG - chr5_3:17240757-17240776 Msa0802680:intron 50.0%
! TAGACCTGTGATGTTGGTGG+TGG + chr5_3:17241114-17241133 None:intergenic 50.0%
AACTGTTCCAGTGCTAGTGG+CGG + chr5_3:17241231-17241250 None:intergenic 50.0%
CTTGACGCCATGAATCCATG+TGG + chr5_3:17241382-17241401 None:intergenic 50.0%
CCCAAGCTCACCCTTATATG+TGG - chr5_3:17241404-17241423 Msa0802680:intron 50.0%
CCCACATATAAGGGTGAGCT+TGG + chr5_3:17241408-17241427 None:intergenic 50.0%
CCTGGACACCCCACATATAA+GGG + chr5_3:17241417-17241436 None:intergenic 50.0%
ACCTGGACACCCCACATATA+AGG + chr5_3:17241418-17241437 None:intergenic 50.0%
CAGCACATGATGCCTCCTTA+TGG - chr5_3:17242415-17242434 Msa0802680:intron 50.0%
! TGTGGTGGAGTACCATAAGG+AGG + chr5_3:17242430-17242449 None:intergenic 50.0%
! GGATGTGGTGGAGTACCATA+AGG + chr5_3:17242433-17242452 None:intergenic 50.0%
GTTGCAATGTATCCCCATGG+TGG - chr5_3:17242457-17242476 Msa0802680:intron 50.0%
! ATGATTACCGGAGGCATGGA+TGG + chr5_3:17242496-17242515 None:intergenic 50.0%
GACAATGATTACCGGAGGCA+TGG + chr5_3:17242500-17242519 None:intergenic 50.0%
GCAGAAGGGGGAAAAACTCA+CGG + chr5_3:17242663-17242682 None:intergenic 50.0%
GGACAAGCCCTTCACAGAAT+AGG + chr5_3:17242724-17242743 None:intergenic 50.0%
GACACCACCACCAACATCAC+AGG - chr5_3:17241107-17241126 Msa0802680:intron 55.0%
GACGCCATGAATCCATGTGG+AGG + chr5_3:17241379-17241398 None:intergenic 55.0%
! CCCTTATATGTGGGGTGTCC+AGG - chr5_3:17241414-17241433 Msa0802680:intron 55.0%
GTTGTCGGACTCTCCAACCA+AGG - chr5_3:17241925-17241944 Msa0802680:intron 55.0%
TGCTCATCCATCCATGCCTC+CGG - chr5_3:17242486-17242505 Msa0802680:intron 55.0%
GAGGCCTCAGCAATACCGTT+TGG + chr5_3:17242641-17242660 None:intergenic 55.0%
GAAGGGGGAAAAACTCACGG+AGG + chr5_3:17242660-17242679 None:intergenic 55.0%
CCATCAGTTTCCATGCTGGC+AGG + chr5_3:17242796-17242815 None:intergenic 55.0%
CCTGCCAGCATGGAAACTGA+TGG - chr5_3:17242793-17242812 Msa0802680:five_prime_UTR 55.0%
ACAGCGGGGACATCTGCTAA+TGG - chr5_3:17242916-17242935 Msa0802680:five_prime_UTR 55.0%
GGAACTCGTGGGTCGTCGTA+CGG + chr5_3:17240243-17240262 None:intergenic 60.0%
GTTCGCGGAGAGGAACTCGT+GGG + chr5_3:17240254-17240273 None:intergenic 60.0%
TGTTCGCGGAGAGGAACTCG+TGG + chr5_3:17240255-17240274 None:intergenic 60.0%
TCCTGACTGGGCCAGCTTTC+AGG - chr5_3:17241253-17241272 Msa0802680:intron 60.0%
ACCTGAAAGCTGGCCCAGTC+AGG + chr5_3:17241257-17241276 None:intergenic 60.0%
GGGAAACACTCCTGCCAGCA+TGG - chr5_3:17242783-17242802 Msa0802680:five_prime_UTR 60.0%
CTCGTGGGTCGTCGTACGGT+GGG + chr5_3:17240239-17240258 None:intergenic 65.0%
ACTCGTGGGTCGTCGTACGG+TGG + chr5_3:17240240-17240259 None:intergenic 65.0%
CCAGTGCTAGTGGCGGTCGA+AGG + chr5_3:17241224-17241243 None:intergenic 65.0%
CCTTCGACCGCCACTAGCAC+TGG - chr5_3:17241221-17241240 Msa0802680:intron 65.0%
TGGCGGTCGAAGGCTGCTCC+TGG + chr5_3:17241214-17241233 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 17240207 17242951 17240207 ID=Msa0802680;Name=Msa0802680
chr5_3 mRNA 17240207 17242951 17240207 ID=Msa0802680-mRNA-1;Parent=Msa0802680;Name=Msa0802680-mRNA-1;_AED=0.15;_eAED=0.15;_QI=358|0.8|0.83|1|0.8|0.5|6|0|148
chr5_3 exon 17240207 17240374 17240207 ID=Msa0802680-mRNA-1:exon:7183;Parent=Msa0802680-mRNA-1
chr5_3 exon 17240497 17240553 17240497 ID=Msa0802680-mRNA-1:exon:7182;Parent=Msa0802680-mRNA-1
chr5_3 exon 17240651 17240740 17240651 ID=Msa0802680-mRNA-1:exon:7181;Parent=Msa0802680-mRNA-1
chr5_3 exon 17241884 17241943 17241884 ID=Msa0802680-mRNA-1:exon:7180;Parent=Msa0802680-mRNA-1
chr5_3 exon 17242030 17242142 17242030 ID=Msa0802680-mRNA-1:exon:7179;Parent=Msa0802680-mRNA-1
chr5_3 exon 17242638 17242951 17242638 ID=Msa0802680-mRNA-1:exon:7178;Parent=Msa0802680-mRNA-1
chr5_3 five_prime_UTR 17242638 17242951 17242638 ID=Msa0802680-mRNA-1:five_prime_utr;Parent=Msa0802680-mRNA-1
chr5_3 five_prime_UTR 17242099 17242142 17242099 ID=Msa0802680-mRNA-1:five_prime_utr;Parent=Msa0802680-mRNA-1
chr5_3 CDS 17242030 17242098 17242030 ID=Msa0802680-mRNA-1:cds;Parent=Msa0802680-mRNA-1
chr5_3 CDS 17241884 17241943 17241884 ID=Msa0802680-mRNA-1:cds;Parent=Msa0802680-mRNA-1
chr5_3 CDS 17240651 17240740 17240651 ID=Msa0802680-mRNA-1:cds;Parent=Msa0802680-mRNA-1
chr5_3 CDS 17240497 17240553 17240497 ID=Msa0802680-mRNA-1:cds;Parent=Msa0802680-mRNA-1
chr5_3 CDS 17240207 17240374 17240207 ID=Msa0802680-mRNA-1:cds;Parent=Msa0802680-mRNA-1
Gene Sequence

>Msa0802680

ATGGGCAATAATGAGATGGATAAAACAGCAAAAGAGAAGGAGTCGAAGACACCACCACCAACATCACAGGAGCAGCCTTCGACCGCCACTAGCACTGGAACAGTTAATCCTGACTGGGCCAGCTTTCAGCACATGATGCCTCCTTATGGTACTCCACCACATCCCTATGTTGCAATGTATCCCCATGGTGGCATATATGCTCATCCATCCATGCCTCCGGGATCTTATCCATATCCTTATGCCATGCCTACTCCAAACGGTATTGCTGAGGCCTCCGGAAACACTCCTGCCAGCATGGAAACTGATGGTAAACCTCCTGAAGTGAAGGAAAAATTACCAATCAAAAGGTCAAAAGGAAGTTTAGGTAGTTTGAACATGATTACAGGGAAAAATAATGAACATGGTAAAACAGCGGGGACATCTGCTAATGGAATTCCCTCAAAG
Protein sequence

>Msa0802680

MGNNEMDKTAKEKESKTPPPTSQEQPSTATSTGTVNPDWASFQHMMPPYGTPPHPYVAMYPHGGIYAHPSMPPGSYPYPYAMPTPNGIAEASGNTPASMETDGKPPEVKEKLPIKRSKGSLGSLNMITGKNNEHGKTAGTSANGIPSK