AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1012810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012810 MtrunA17_Chr7g0224361 99.422 173 1 0 1 173 1 173 1.81e-127 355
Msa1012810 MtrunA17_Chr7g0214211 48.322 149 68 5 33 173 26 173 8.58e-43 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012810 AT3G26960.1 52.381 147 63 3 29 172 30 172 3.82e-54 169
Msa1012810 AT5G41050.1 52.448 143 65 1 33 172 29 171 9.80e-54 168
Msa1012810 AT5G13140.1 48.611 144 71 1 33 173 35 178 2.11e-48 158

Find 25 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTTTCCCTAGCACTTT+TGG 0.216215 7_1:+18238918 None:intergenic
TCTTGCCAAGCAAATTTAAT+AGG 0.232515 7_1:-18238199 Msa1012810:CDS
AACAGAACAACAAACAAATA+TGG 0.325312 7_1:-18238289 Msa1012810:CDS
CAAGAGAGTTAGAGGATTCA+TGG 0.327947 7_1:+18238989 None:intergenic
ACCACTGCTTGCAATGTTCC+TGG 0.329830 7_1:-18238169 Msa1012810:CDS
CTTGCCAAGCAAATTTAATA+GGG 0.333086 7_1:-18238198 Msa1012810:CDS
AAAATAAGCAAGAGAGTTAG+AGG 0.355066 7_1:+18238981 None:intergenic
ATTTGAGAGTTAGGAAATCT+TGG 0.382666 7_1:+18238900 None:intergenic
AAGACTATATATGCAGAGAT+TGG 0.392393 7_1:+18238102 None:intergenic
AACTCCCTATTAAATTTGCT+TGG 0.406122 7_1:+18238194 None:intergenic
ATCACACTGATTTGAGAGTT+AGG 0.427686 7_1:+18238891 None:intergenic
ATTGAAGTACCATCAGTTGA+TGG 0.490136 7_1:-18238256 Msa1012810:CDS
AGACATAGCTACTTCTTGCC+AGG 0.496803 7_1:-18238825 Msa1012810:intron
AGACCAGTGTCCTTCTTAGA+TGG 0.511274 7_1:+18238061 None:intergenic
AACTCTCAAATCAGTGTGAT+GGG 0.515882 7_1:-18238888 Msa1012810:CDS
TAACTCTCAAATCAGTGTGA+TGG 0.521454 7_1:-18238889 Msa1012810:CDS
ATAATTATGTTTAGGTGCTG+AGG 0.544339 7_1:-18238377 Msa1012810:intron
CCTGCAAGACCAAAAGTGCT+AGG 0.546586 7_1:-18238927 Msa1012810:CDS
CAAACAAATATGGAATGTAC+AGG 0.559679 7_1:-18238279 Msa1012810:CDS
CATCTCACTCCATCAACTGA+TGG 0.589191 7_1:+18238247 None:intergenic
AAGAAGGACACTGGTCTATG+TGG 0.605551 7_1:-18238055 Msa1012810:CDS
TCCAGGAACATTGCAAGCAG+TGG 0.607145 7_1:+18238168 None:intergenic
CTGCAAGACCAAAAGTGCTA+GGG 0.608365 7_1:-18238926 Msa1012810:CDS
AGACCATCTAAGAAGGACAC+TGG 0.644130 7_1:-18238064 Msa1012810:CDS
GATGGAGTGAGATGTGCTGA+AGG 0.665515 7_1:-18238238 Msa1012810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTTAAAATTTTACG+AGG + chr7_1:18238432-18238451 None:intergenic 15.0%
!! TTTCTTTCATAATTATGTTT+AGG - chr7_1:18238650-18238669 Msa1012810:intron 15.0%
!!! TGTTTTTTTAAAATTTTACG+AGG + chr7_1:18238432-18238451 None:intergenic 15.0%
!! TTTCTTTCATAATTATGTTT+AGG - chr7_1:18238650-18238669 Msa1012810:intron 15.0%
! GGTTATTCAAAATTATTACC+TGG + chr7_1:18238231-18238250 None:intergenic 25.0%
! TTGTAAAATTGTCATTGCTA+TGG + chr7_1:18238352-18238371 None:intergenic 25.0%
!!! CATTTTGTAACTTGTTGTAA+AGG - chr7_1:18238466-18238485 Msa1012810:intron 25.0%
!! TAGTTTAACTTTCGTAGAAA+TGG + chr7_1:18238578-18238597 None:intergenic 25.0%
! AACAGAACAACAAACAAATA+TGG - chr7_1:18238746-18238765 Msa1012810:intron 25.0%
!! AAATTTTAGACCATCTAAGA+AGG - chr7_1:18238964-18238983 Msa1012810:CDS 25.0%
! GGTTATTCAAAATTATTACC+TGG + chr7_1:18238231-18238250 None:intergenic 25.0%
! TTGTAAAATTGTCATTGCTA+TGG + chr7_1:18238352-18238371 None:intergenic 25.0%
!!! CATTTTGTAACTTGTTGTAA+AGG - chr7_1:18238466-18238485 Msa1012810:intron 25.0%
!! TAGTTTAACTTTCGTAGAAA+TGG + chr7_1:18238578-18238597 None:intergenic 25.0%
! AACAGAACAACAAACAAATA+TGG - chr7_1:18238746-18238765 Msa1012810:intron 25.0%
!! AAATTTTAGACCATCTAAGA+AGG - chr7_1:18238964-18238983 Msa1012810:CDS 25.0%
AAAATAAGCAAGAGAGTTAG+AGG + chr7_1:18238057-18238076 None:intergenic 30.0%
! ACCTTTCTTTACTCAGTTTT+TGG - chr7_1:18238077-18238096 Msa1012810:CDS 30.0%
TCCAAAAACTGAGTAAAGAA+AGG + chr7_1:18238081-18238100 None:intergenic 30.0%
ATTTGAGAGTTAGGAAATCT+TGG + chr7_1:18238138-18238157 None:intergenic 30.0%
ATGTAAACAACTAACACTAG+AGG + chr7_1:18238252-18238271 None:intergenic 30.0%
!!! TTTTTTTGTTGTCAACTCAG+TGG + chr7_1:18238604-18238623 None:intergenic 30.0%
! ATAATTATGTTTAGGTGCTG+AGG - chr7_1:18238658-18238677 Msa1012810:intron 30.0%
CAAACAAATATGGAATGTAC+AGG - chr7_1:18238756-18238775 Msa1012810:intron 30.0%
TCTTGCCAAGCAAATTTAAT+AGG - chr7_1:18238836-18238855 Msa1012810:CDS 30.0%
CTTGCCAAGCAAATTTAATA+GGG - chr7_1:18238837-18238856 Msa1012810:CDS 30.0%
! AACTCCCTATTAAATTTGCT+TGG + chr7_1:18238844-18238863 None:intergenic 30.0%
AAGACTATATATGCAGAGAT+TGG + chr7_1:18238936-18238955 None:intergenic 30.0%
AAAATAAGCAAGAGAGTTAG+AGG + chr7_1:18238057-18238076 None:intergenic 30.0%
! ACCTTTCTTTACTCAGTTTT+TGG - chr7_1:18238077-18238096 Msa1012810:CDS 30.0%
TCCAAAAACTGAGTAAAGAA+AGG + chr7_1:18238081-18238100 None:intergenic 30.0%
ATTTGAGAGTTAGGAAATCT+TGG + chr7_1:18238138-18238157 None:intergenic 30.0%
ATGTAAACAACTAACACTAG+AGG + chr7_1:18238252-18238271 None:intergenic 30.0%
!!! TTTTTTTGTTGTCAACTCAG+TGG + chr7_1:18238604-18238623 None:intergenic 30.0%
! ATAATTATGTTTAGGTGCTG+AGG - chr7_1:18238658-18238677 Msa1012810:intron 30.0%
CAAACAAATATGGAATGTAC+AGG - chr7_1:18238756-18238775 Msa1012810:intron 30.0%
TCTTGCCAAGCAAATTTAAT+AGG - chr7_1:18238836-18238855 Msa1012810:CDS 30.0%
CTTGCCAAGCAAATTTAATA+GGG - chr7_1:18238837-18238856 Msa1012810:CDS 30.0%
! AACTCCCTATTAAATTTGCT+TGG + chr7_1:18238844-18238863 None:intergenic 30.0%
AAGACTATATATGCAGAGAT+TGG + chr7_1:18238936-18238955 None:intergenic 30.0%
!! ATCACACTGATTTGAGAGTT+AGG + chr7_1:18238147-18238166 None:intergenic 35.0%
TAACTCTCAAATCAGTGTGA+TGG - chr7_1:18238146-18238165 Msa1012810:CDS 35.0%
AACTCTCAAATCAGTGTGAT+GGG - chr7_1:18238147-18238166 Msa1012810:CDS 35.0%
! ATTGAAGTACCATCAGTTGA+TGG - chr7_1:18238779-18238798 Msa1012810:intron 35.0%
!! ATCACACTGATTTGAGAGTT+AGG + chr7_1:18238147-18238166 None:intergenic 35.0%
TAACTCTCAAATCAGTGTGA+TGG - chr7_1:18238146-18238165 Msa1012810:CDS 35.0%
AACTCTCAAATCAGTGTGAT+GGG - chr7_1:18238147-18238166 Msa1012810:CDS 35.0%
! ATTGAAGTACCATCAGTTGA+TGG - chr7_1:18238779-18238798 Msa1012810:intron 35.0%
! TTAGCAGTGCTTTTGTATCC+AGG + chr7_1:18238887-18238906 None:intergenic 40.0%
! TTAGCAGTGCTTTTGTATCC+AGG + chr7_1:18238887-18238906 None:intergenic 40.0%
!! CTGCAAGACCAAAAGTGCTA+GGG - chr7_1:18238109-18238128 Msa1012810:CDS 45.0%
! CTTGGTTTCCCTAGCACTTT+TGG + chr7_1:18238120-18238139 None:intergenic 45.0%
AGACATAGCTACTTCTTGCC+AGG - chr7_1:18238210-18238229 Msa1012810:CDS 45.0%
CATCTCACTCCATCAACTGA+TGG + chr7_1:18238791-18238810 None:intergenic 45.0%
AGACCATCTAAGAAGGACAC+TGG - chr7_1:18238971-18238990 Msa1012810:CDS 45.0%
AGACCAGTGTCCTTCTTAGA+TGG + chr7_1:18238977-18238996 None:intergenic 45.0%
! AAGAAGGACACTGGTCTATG+TGG - chr7_1:18238980-18238999 Msa1012810:CDS 45.0%
!! CTGCAAGACCAAAAGTGCTA+GGG - chr7_1:18238109-18238128 Msa1012810:CDS 45.0%
! CTTGGTTTCCCTAGCACTTT+TGG + chr7_1:18238120-18238139 None:intergenic 45.0%
AGACATAGCTACTTCTTGCC+AGG - chr7_1:18238210-18238229 Msa1012810:CDS 45.0%
CATCTCACTCCATCAACTGA+TGG + chr7_1:18238791-18238810 None:intergenic 45.0%
AGACCATCTAAGAAGGACAC+TGG - chr7_1:18238971-18238990 Msa1012810:CDS 45.0%
AGACCAGTGTCCTTCTTAGA+TGG + chr7_1:18238977-18238996 None:intergenic 45.0%
! AAGAAGGACACTGGTCTATG+TGG - chr7_1:18238980-18238999 Msa1012810:CDS 45.0%
!!! TCTTTAAAAAAAAAAAAATT+TGG - chr7_1:18238305-18238324 Msa1012810:CDS 5.0%
!!! TCTTTAAAAAAAAAAAAATT+TGG - chr7_1:18238305-18238324 Msa1012810:CDS 5.0%
!!! CCTAGCACTTTTGGTCTTGC+AGG + chr7_1:18238111-18238130 None:intergenic 50.0%
!! CCTGCAAGACCAAAAGTGCT+AGG - chr7_1:18238108-18238127 Msa1012810:CDS 50.0%
GATGGAGTGAGATGTGCTGA+AGG - chr7_1:18238797-18238816 Msa1012810:intron 50.0%
ACCACTGCTTGCAATGTTCC+TGG - chr7_1:18238866-18238885 Msa1012810:CDS 50.0%
TCCAGGAACATTGCAAGCAG+TGG + chr7_1:18238870-18238889 None:intergenic 50.0%
!!! CCTAGCACTTTTGGTCTTGC+AGG + chr7_1:18238111-18238130 None:intergenic 50.0%
!! CCTGCAAGACCAAAAGTGCT+AGG - chr7_1:18238108-18238127 Msa1012810:CDS 50.0%
GATGGAGTGAGATGTGCTGA+AGG - chr7_1:18238797-18238816 Msa1012810:intron 50.0%
ACCACTGCTTGCAATGTTCC+TGG - chr7_1:18238866-18238885 Msa1012810:CDS 50.0%
TCCAGGAACATTGCAAGCAG+TGG + chr7_1:18238870-18238889 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 18238048 18239009 18238048 ID=Msa1012810;Name=Msa1012810
chr7_1 mRNA 18238048 18239009 18238048 ID=Msa1012810-mRNA-1;Parent=Msa1012810;Name=Msa1012810-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|173
chr7_1 exon 18238826 18239009 18238826 ID=Msa1012810-mRNA-1:exon:4575;Parent=Msa1012810-mRNA-1
chr7_1 exon 18238048 18238385 18238048 ID=Msa1012810-mRNA-1:exon:4574;Parent=Msa1012810-mRNA-1
chr7_1 CDS 18238826 18239009 18238826 ID=Msa1012810-mRNA-1:cds;Parent=Msa1012810-mRNA-1
chr7_1 CDS 18238048 18238385 18238048 ID=Msa1012810-mRNA-1:cds;Parent=Msa1012810-mRNA-1
Gene Sequence

>Msa1012810

ATGAATCCTCTAACTCTCTTGCTTATTTTACCTTTCTTTACTCAGTTTTTGGAAGTTGAACCTGCAAGACCAAAAGTGCTAGGGAAACCAAGATTTCCTAACTCTCAAATCAGTGTGATGGGCTTTGTTTATTGTGATTTCTGCTCAAACAACAGCTTTTCTAGACATAGCTACTTCTTGCCAGGTGCTGAGGTGAAAGTAGATTGCATGTTCAAAGCAATTTCAGCAAAAACGACAGAGCAGATTACACTGTCAGTGAACAGAACAACAAACAAATATGGAATGTACAGGATTGAAGTACCATCAGTTGATGGAGTGAGATGTGCTGAAGGAACTGAAGTGATGTCTTCTTGCCAAGCAAATTTAATAGGGAGTTCAACCACTGCTTGCAATGTTCCTGGATACAAAAGCACTGCTAATGTGATATCAGTCAAAGCAAGAAAAACCAATCTCTGCATATATAGTCTTAATGCTTTAAATTTTAGACCATCTAAGAAGGACACTGGTCTATGTGGTAATTGA

Protein sequence

>Msa1012810

MNPLTLLLILPFFTQFLEVEPARPKVLGKPRFPNSQISVMGFVYCDFCSNNSFSRHSYFLPGAEVKVDCMFKAISAKTTEQITLSVNRTTNKYGMYRIEVPSVDGVRCAEGTEVMSSCQANLIGSSTTACNVPGYKSTANVISVKARKTNLCIYSLNALNFRPSKKDTGLCGN*