AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0813420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0813420 MtrunA17_Chr5g0430631 77.778 45 2 1 47 91 13 49 2.67e-17 70.5
Msa0813420 MtrunA17_Chr1g0146881 84.375 32 5 0 1 32 144 175 5.89e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATCAAATTATTGCTTTA+TGG 0.104292 5_3:+44203036 Msa0813420:three_prime_UTR
TGAAGCATCCTCTTGCTTTC+AGG 0.176242 5_3:-44201590 None:intergenic
GTACTAATGTTGCTGAAATA+AGG 0.197501 5_3:+44202867 Msa0813420:three_prime_UTR
CCATTGTTCACTGCCTTCTT+TGG 0.244312 5_3:-44202732 None:intergenic
TATTGTTTGCCTATTGAAAC+AGG 0.352905 5_3:+44200725 Msa0813420:five_prime_UTR
GTTTGCCCTAAGATTTGATT+AGG 0.353571 5_3:+44200672 None:intergenic
GCCAAGGAGTGAACTCTATC+AGG 0.356417 5_3:-44200782 None:intergenic
TTTGTGCCGGACAATGTTAA+TGG 0.404700 5_3:+44201544 Msa0813420:CDS
TACTAATGTTGCTGAAATAA+GGG 0.414247 5_3:+44202868 Msa0813420:three_prime_UTR
TTATTCGTTGGAATTAAGTT+CGG 0.414784 5_3:-44202771 None:intergenic
TTGGCTTTGCTAAACGCAAC+GGG 0.440138 5_3:+44200800 Msa0813420:CDS
ATCATAGATCCTGTTTCAAT+AGG 0.442035 5_3:-44200734 None:intergenic
CATCTCTTGTGAAACTGAAC+AGG 0.443604 5_3:+44201759 Msa0813420:CDS
TTTCAACATCTGTTATTCGT+TGG 0.452009 5_3:-44202783 None:intergenic
TCCTGATAGAGTTCACTCCT+TGG 0.452816 5_3:+44200781 Msa0813420:CDS
CTTGATCCATTAACATTGTC+CGG 0.456743 5_3:-44201550 None:intergenic
AGGACAATCTCCTAAGGCTT+AGG 0.474201 5_3:-44202824 None:intergenic
TATAGCTTCTCCTAAGCCTT+AGG 0.477644 5_3:+44202814 Msa0813420:three_prime_UTR
TGTCCGGCACAAACATGGTT+GGG 0.482898 5_3:-44201534 None:intergenic
GCAAGGGATTGAATGTACTT+TGG 0.513385 5_3:+44202693 Msa0813420:exon
ATTAAGTTCGGCTCATGTGT+TGG 0.516857 5_3:-44202759 None:intergenic
TGAACCACAGAAACTATAAG+AGG 0.523659 5_3:+44201615 Msa0813420:CDS
ATCACCTCTTATAGTTTCTG+TGG 0.529669 5_3:-44201619 None:intergenic
TTGTCCGGCACAAACATGGT+TGG 0.549962 5_3:-44201535 None:intergenic
CTTGGCTTTGCTAAACGCAA+CGG 0.553494 5_3:+44200799 Msa0813420:CDS
AGCTTGTAAAATAGCAGCAA+TGG 0.557695 5_3:+44200757 Msa0813420:CDS
TAGTAGATATTGTACAATCA+AGG 0.559319 5_3:-44202844 None:intergenic
CAATCAAGGACAATCTCCTA+AGG 0.561772 5_3:-44202830 None:intergenic
AACATTGTCCGGCACAAACA+TGG 0.571085 5_3:-44201539 None:intergenic
AGTCCCAACCATGTTTGTGC+CGG 0.582912 5_3:+44201531 Msa0813420:CDS
TGTTTGTGTCAGATATGTTG+TGG 0.598784 5_3:+44201498 Msa0813420:intron
GACATTGTTTGGAACAACCA+AGG 0.606311 5_3:-44202966 None:intergenic
CTTGCTTTCAGGAAAAGCAG+TGG 0.613067 5_3:-44201579 None:intergenic
CCAAAGAAGGCAGTGAACAA+TGG 0.620768 5_3:+44202732 Msa0813420:three_prime_UTR
CAAACTTAGTTCAGGACGAG+TGG 0.621875 5_3:+44200977 Msa0813420:intron
CGTTGCGTTTAGCAAAGCCA+AGG 0.625437 5_3:-44200798 None:intergenic
GCTTTGCTAAACGCAACGGG+TGG 0.631397 5_3:+44200803 Msa0813420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTGATTTATTTTTT+CGG + chr5_3:44200874-44200893 Msa0813420:intron 10.0%
!! ATTTAAATTAGAAAGTAATA+TGG - chr5_3:44201253-44201272 None:intergenic 10.0%
!! TAAATATATTATACATGAAA+GGG - chr5_3:44202204-44202223 None:intergenic 10.0%
!! ATAAATATATTATACATGAA+AGG - chr5_3:44202205-44202224 None:intergenic 10.0%
!!! CAATACATTTTTTATTTAAA+AGG + chr5_3:44202988-44203007 Msa0813420:three_prime_UTR 10.0%
!!! AACATTTTGATTTATTTTTT+CGG + chr5_3:44200874-44200893 Msa0813420:intron 10.0%
!! ATTTAAATTAGAAAGTAATA+TGG - chr5_3:44201253-44201272 None:intergenic 10.0%
!! TAAATATATTATACATGAAA+GGG - chr5_3:44202204-44202223 None:intergenic 10.0%
!! ATAAATATATTATACATGAA+AGG - chr5_3:44202205-44202224 None:intergenic 10.0%
!!! CAATACATTTTTTATTTAAA+AGG + chr5_3:44202988-44203007 Msa0813420:three_prime_UTR 10.0%
!! AATGTTCAAAGAATTATAAA+AGG - chr5_3:44200860-44200879 None:intergenic 15.0%
!!! AGATTTTTAAAGAATATTGA+CGG + chr5_3:44201119-44201138 Msa0813420:intron 15.0%
!! TAATTTAAATTTCTATATGC+AGG + chr5_3:44201263-44201282 Msa0813420:intron 15.0%
!! TATATTAAAGTTAATGCTAT+AGG - chr5_3:44202088-44202107 None:intergenic 15.0%
!! TAATATATTTATGAAAGAGT+CGG + chr5_3:44202213-44202232 Msa0813420:intron 15.0%
!!! TAAAATAAATTAGTTTGACA+AGG - chr5_3:44202427-44202446 None:intergenic 15.0%
!!! GATAGAATTTTAAATATGTT+TGG - chr5_3:44202586-44202605 None:intergenic 15.0%
!!! TATTTAAAATTCTATCCTTA+TGG + chr5_3:44202590-44202609 Msa0813420:intron 15.0%
!!! TAAAGGAATTTAATGTATTT+TGG + chr5_3:44202658-44202677 Msa0813420:intron 15.0%
!! TTAATCAAATTATTGCTTTA+TGG + chr5_3:44203036-44203055 Msa0813420:three_prime_UTR 15.0%
!! AATGTTCAAAGAATTATAAA+AGG - chr5_3:44200860-44200879 None:intergenic 15.0%
!!! AGATTTTTAAAGAATATTGA+CGG + chr5_3:44201119-44201138 Msa0813420:intron 15.0%
!! TAATTTAAATTTCTATATGC+AGG + chr5_3:44201263-44201282 Msa0813420:intron 15.0%
!! TATATTAAAGTTAATGCTAT+AGG - chr5_3:44202088-44202107 None:intergenic 15.0%
!! TAATATATTTATGAAAGAGT+CGG + chr5_3:44202213-44202232 Msa0813420:intron 15.0%
!!! TAAAATAAATTAGTTTGACA+AGG - chr5_3:44202427-44202446 None:intergenic 15.0%
!!! GATAGAATTTTAAATATGTT+TGG - chr5_3:44202586-44202605 None:intergenic 15.0%
!!! TATTTAAAATTCTATCCTTA+TGG + chr5_3:44202590-44202609 Msa0813420:intron 15.0%
!!! TAAAGGAATTTAATGTATTT+TGG + chr5_3:44202658-44202677 Msa0813420:intron 15.0%
!! TTAATCAAATTATTGCTTTA+TGG + chr5_3:44203036-44203055 Msa0813420:three_prime_UTR 15.0%
!! AAGTTTGTAATTAAAAAGTG+TGG - chr5_3:44200964-44200983 None:intergenic 20.0%
!! TTTAATTACAAACTTAGTTC+AGG + chr5_3:44200969-44200988 Msa0813420:intron 20.0%
!! GTAAAATTATTGTGAGAATT+GGG + chr5_3:44201326-44201345 Msa0813420:intron 20.0%
!!! CACTTTGTTATCAAATAAAA+TGG - chr5_3:44201674-44201693 None:intergenic 20.0%
!!! TCATTCCAATAAACAAATTT+TGG - chr5_3:44201914-44201933 None:intergenic 20.0%
!!! ATTTTTTTTTCTTTGCCATA+AGG - chr5_3:44202608-44202627 None:intergenic 20.0%
!! AATAGTATATTGTAGATGTA+TGG + chr5_3:44202625-44202644 Msa0813420:intron 20.0%
!!! AAAAATGTATTGACATTGTT+TGG - chr5_3:44202980-44202999 None:intergenic 20.0%
!! AAGTTTGTAATTAAAAAGTG+TGG - chr5_3:44200964-44200983 None:intergenic 20.0%
!! TTTAATTACAAACTTAGTTC+AGG + chr5_3:44200969-44200988 Msa0813420:intron 20.0%
!! GTAAAATTATTGTGAGAATT+GGG + chr5_3:44201326-44201345 Msa0813420:intron 20.0%
!!! CACTTTGTTATCAAATAAAA+TGG - chr5_3:44201674-44201693 None:intergenic 20.0%
!!! TCATTCCAATAAACAAATTT+TGG - chr5_3:44201914-44201933 None:intergenic 20.0%
!!! ATTTTTTTTTCTTTGCCATA+AGG - chr5_3:44202608-44202627 None:intergenic 20.0%
!! AATAGTATATTGTAGATGTA+TGG + chr5_3:44202625-44202644 Msa0813420:intron 20.0%
!!! AAAAATGTATTGACATTGTT+TGG - chr5_3:44202980-44202999 None:intergenic 20.0%
! TTAACTACATTAATGATTGC+TGG + chr5_3:44200938-44200957 Msa0813420:intron 25.0%
! GTTGAGAATTTATCTTTCTA+TGG + chr5_3:44201034-44201053 Msa0813420:intron 25.0%
! GGTAAAATTATTGTGAGAAT+TGG + chr5_3:44201325-44201344 Msa0813420:intron 25.0%
! ATGCAACAAATCAAAATTCT+TGG + chr5_3:44201381-44201400 Msa0813420:intron 25.0%
! TGCTAATCTACTAAAATACA+TGG - chr5_3:44201716-44201735 None:intergenic 25.0%
!! ACTTATTTCTCTTTGAACTA+TGG + chr5_3:44201786-44201805 Msa0813420:intron 25.0%
!! GGTTTCCAAAATTTGTTTAT+TGG + chr5_3:44201906-44201925 Msa0813420:intron 25.0%
!!! ATTTTTGTCTGAGACAAAAT+CGG + chr5_3:44201974-44201993 Msa0813420:intron 25.0%
! TAACGATAATCTGACAAATT+AGG - chr5_3:44202013-44202032 None:intergenic 25.0%
! TTATGGTTAATTTCAAGGTA+AGG + chr5_3:44202357-44202376 Msa0813420:intron 25.0%
! ATACATTTCGAAACTTTAGT+CGG + chr5_3:44202449-44202468 Msa0813420:intron 25.0%
!! ATGTTTGGTGAAAAAATGAT+CGG - chr5_3:44202571-44202590 None:intergenic 25.0%
! TGTATGGTAAGATTTGATAA+AGG + chr5_3:44202641-44202660 Msa0813420:intron 25.0%
! TTATTCGTTGGAATTAAGTT+CGG - chr5_3:44202774-44202793 None:intergenic 25.0%
! TAGTAGATATTGTACAATCA+AGG - chr5_3:44202847-44202866 None:intergenic 25.0%
! TACTAATGTTGCTGAAATAA+GGG + chr5_3:44202868-44202887 Msa0813420:three_prime_UTR 25.0%
! TTAACTACATTAATGATTGC+TGG + chr5_3:44200938-44200957 Msa0813420:intron 25.0%
! GTTGAGAATTTATCTTTCTA+TGG + chr5_3:44201034-44201053 Msa0813420:intron 25.0%
! GGTAAAATTATTGTGAGAAT+TGG + chr5_3:44201325-44201344 Msa0813420:intron 25.0%
! ATGCAACAAATCAAAATTCT+TGG + chr5_3:44201381-44201400 Msa0813420:intron 25.0%
! TGCTAATCTACTAAAATACA+TGG - chr5_3:44201716-44201735 None:intergenic 25.0%
!! ACTTATTTCTCTTTGAACTA+TGG + chr5_3:44201786-44201805 Msa0813420:intron 25.0%
!! GGTTTCCAAAATTTGTTTAT+TGG + chr5_3:44201906-44201925 Msa0813420:intron 25.0%
!!! ATTTTTGTCTGAGACAAAAT+CGG + chr5_3:44201974-44201993 Msa0813420:intron 25.0%
! TAACGATAATCTGACAAATT+AGG - chr5_3:44202013-44202032 None:intergenic 25.0%
! TTATGGTTAATTTCAAGGTA+AGG + chr5_3:44202357-44202376 Msa0813420:intron 25.0%
! ATACATTTCGAAACTTTAGT+CGG + chr5_3:44202449-44202468 Msa0813420:intron 25.0%
!! ATGTTTGGTGAAAAAATGAT+CGG - chr5_3:44202571-44202590 None:intergenic 25.0%
! TGTATGGTAAGATTTGATAA+AGG + chr5_3:44202641-44202660 Msa0813420:intron 25.0%
! TTATTCGTTGGAATTAAGTT+CGG - chr5_3:44202774-44202793 None:intergenic 25.0%
! TAGTAGATATTGTACAATCA+AGG - chr5_3:44202847-44202866 None:intergenic 25.0%
! TACTAATGTTGCTGAAATAA+GGG + chr5_3:44202868-44202887 Msa0813420:three_prime_UTR 25.0%
TATTGTTTGCCTATTGAAAC+AGG + chr5_3:44200725-44200744 Msa0813420:five_prime_UTR 30.0%
ATCATAGATCCTGTTTCAAT+AGG - chr5_3:44200737-44200756 None:intergenic 30.0%
! AGATTTTCAATGTTTTCCAG+AGG + chr5_3:44200826-44200845 Msa0813420:CDS 30.0%
! ATAAAAGGTACATATACCTC+TGG - chr5_3:44200845-44200864 None:intergenic 30.0%
CTCAACTAAATTTGTCCTAA+AGG - chr5_3:44201020-44201039 None:intergenic 30.0%
TATTTCTCAAGACAGTTATC+TGG + chr5_3:44201181-44201200 Msa0813420:intron 30.0%
! CAGCTTCAATTCTAAAGATA+TGG - chr5_3:44201352-44201371 None:intergenic 30.0%
! ACTATAAGAGGTGATGATTT+TGG + chr5_3:44201627-44201646 Msa0813420:intron 30.0%
!! TTTTGGAAACCACGAATATT+GGG - chr5_3:44201897-44201916 None:intergenic 30.0%
!! ATTTTGGAAACCACGAATAT+TGG - chr5_3:44201898-44201917 None:intergenic 30.0%
CATATCAATACCAACATCAA+CGG - chr5_3:44202050-44202069 None:intergenic 30.0%
ATGTATCCATGAAAATATGC+AGG + chr5_3:44202153-44202172 Msa0813420:intron 30.0%
ATGGTCCTTCATTTATGAAA+TGG + chr5_3:44202178-44202197 Msa0813420:intron 30.0%
CGACGTTGTTAAACAATATT+AGG + chr5_3:44202279-44202298 Msa0813420:intron 30.0%
!! TTAGTCGGCTTTCTTTTTAT+TGG + chr5_3:44202464-44202483 Msa0813420:intron 30.0%
!!! TGTATTTTGGTCATACATAC+AGG + chr5_3:44202671-44202690 Msa0813420:intron 30.0%
!! TCTTTGGAGATTGATTTTTC+AGG - chr5_3:44202719-44202738 None:intergenic 30.0%
GAAAAATCAATCTCCAAAGA+AGG + chr5_3:44202719-44202738 Msa0813420:three_prime_UTR 30.0%
TTTCAACATCTGTTATTCGT+TGG - chr5_3:44202786-44202805 None:intergenic 30.0%
GTACTAATGTTGCTGAAATA+AGG + chr5_3:44202867-44202886 Msa0813420:three_prime_UTR 30.0%
AAAAACTACACTAGAGGAAT+GGG - chr5_3:44202915-44202934 None:intergenic 30.0%
AAAAAACTACACTAGAGGAA+TGG - chr5_3:44202916-44202935 None:intergenic 30.0%
TATTGTTTGCCTATTGAAAC+AGG + chr5_3:44200725-44200744 Msa0813420:five_prime_UTR 30.0%
ATCATAGATCCTGTTTCAAT+AGG - chr5_3:44200737-44200756 None:intergenic 30.0%
! AGATTTTCAATGTTTTCCAG+AGG + chr5_3:44200826-44200845 Msa0813420:CDS 30.0%
! ATAAAAGGTACATATACCTC+TGG - chr5_3:44200845-44200864 None:intergenic 30.0%
CTCAACTAAATTTGTCCTAA+AGG - chr5_3:44201020-44201039 None:intergenic 30.0%
TATTTCTCAAGACAGTTATC+TGG + chr5_3:44201181-44201200 Msa0813420:intron 30.0%
! CAGCTTCAATTCTAAAGATA+TGG - chr5_3:44201352-44201371 None:intergenic 30.0%
! ACTATAAGAGGTGATGATTT+TGG + chr5_3:44201627-44201646 Msa0813420:intron 30.0%
!! TTTTGGAAACCACGAATATT+GGG - chr5_3:44201897-44201916 None:intergenic 30.0%
!! ATTTTGGAAACCACGAATAT+TGG - chr5_3:44201898-44201917 None:intergenic 30.0%
CATATCAATACCAACATCAA+CGG - chr5_3:44202050-44202069 None:intergenic 30.0%
ATGTATCCATGAAAATATGC+AGG + chr5_3:44202153-44202172 Msa0813420:intron 30.0%
ATGGTCCTTCATTTATGAAA+TGG + chr5_3:44202178-44202197 Msa0813420:intron 30.0%
CGACGTTGTTAAACAATATT+AGG + chr5_3:44202279-44202298 Msa0813420:intron 30.0%
!! TTAGTCGGCTTTCTTTTTAT+TGG + chr5_3:44202464-44202483 Msa0813420:intron 30.0%
!!! TGTATTTTGGTCATACATAC+AGG + chr5_3:44202671-44202690 Msa0813420:intron 30.0%
!! TCTTTGGAGATTGATTTTTC+AGG - chr5_3:44202719-44202738 None:intergenic 30.0%
GAAAAATCAATCTCCAAAGA+AGG + chr5_3:44202719-44202738 Msa0813420:three_prime_UTR 30.0%
TTTCAACATCTGTTATTCGT+TGG - chr5_3:44202786-44202805 None:intergenic 30.0%
GTACTAATGTTGCTGAAATA+AGG + chr5_3:44202867-44202886 Msa0813420:three_prime_UTR 30.0%
AAAAACTACACTAGAGGAAT+GGG - chr5_3:44202915-44202934 None:intergenic 30.0%
AAAAAACTACACTAGAGGAA+TGG - chr5_3:44202916-44202935 None:intergenic 30.0%
AGCTTGTAAAATAGCAGCAA+TGG + chr5_3:44200757-44200776 Msa0813420:CDS 35.0%
!! CTGATTTTAGGTGATCCTTT+AGG + chr5_3:44201002-44201021 Msa0813420:intron 35.0%
TTGAGAAATACTTCACAGGT+TGG - chr5_3:44201171-44201190 None:intergenic 35.0%
TGTCTTGAGAAATACTTCAC+AGG - chr5_3:44201175-44201194 None:intergenic 35.0%
TATGGTGATAAGAAGATGCA+GGG - chr5_3:44201235-44201254 None:intergenic 35.0%
ATATGGTGATAAGAAGATGC+AGG - chr5_3:44201236-44201255 None:intergenic 35.0%
! TTGCTTTCTCAGTTTTTGTG+TGG + chr5_3:44201292-44201311 Msa0813420:CDS 35.0%
! GTTTTTGTGTGGAATGTCTA+CGG + chr5_3:44201303-44201322 Msa0813420:CDS 35.0%
! TTTTTGTGTGGAATGTCTAC+GGG + chr5_3:44201304-44201323 Msa0813420:CDS 35.0%
TGTGTATACAGCTCTTCATT+AGG + chr5_3:44201422-44201441 Msa0813420:intron 35.0%
TATACAGCTCTTCATTAGGA+AGG + chr5_3:44201426-44201445 Msa0813420:intron 35.0%
TGTTTGTGTCAGATATGTTG+TGG + chr5_3:44201498-44201517 Msa0813420:intron 35.0%
CTTGATCCATTAACATTGTC+CGG - chr5_3:44201553-44201572 None:intergenic 35.0%
TGAACCACAGAAACTATAAG+AGG + chr5_3:44201615-44201634 Msa0813420:CDS 35.0%
ATCACCTCTTATAGTTTCTG+TGG - chr5_3:44201622-44201641 None:intergenic 35.0%
TATGGATATTGTTGAATGCG+AGG + chr5_3:44201804-44201823 Msa0813420:intron 35.0%
CCACGAATATTCAAAAACTC+AGG - chr5_3:44201871-44201890 None:intergenic 35.0%
! CCTGAGTTTTTGAATATTCG+TGG + chr5_3:44201868-44201887 Msa0813420:intron 35.0%
!! CGTTGATGTTGGTATTGATA+TGG + chr5_3:44202048-44202067 Msa0813420:intron 35.0%
AAGGGCCATTTCATAAATGA+AGG - chr5_3:44202186-44202205 None:intergenic 35.0%
AGCAAAGAGCTATCAGTTAA+AGG - chr5_3:44202335-44202354 None:intergenic 35.0%
TTTAACTGATAGCTCTTTGC+TGG + chr5_3:44202334-44202353 Msa0813420:intron 35.0%
GCTGGTTATGGTTAATTTCA+AGG + chr5_3:44202352-44202371 Msa0813420:intron 35.0%
! TAATCAGACATTTTGTGCCT+TGG + chr5_3:44202949-44202968 Msa0813420:three_prime_UTR 35.0%
AGCTTGTAAAATAGCAGCAA+TGG + chr5_3:44200757-44200776 Msa0813420:CDS 35.0%
!! CTGATTTTAGGTGATCCTTT+AGG + chr5_3:44201002-44201021 Msa0813420:intron 35.0%
TTGAGAAATACTTCACAGGT+TGG - chr5_3:44201171-44201190 None:intergenic 35.0%
TGTCTTGAGAAATACTTCAC+AGG - chr5_3:44201175-44201194 None:intergenic 35.0%
TATGGTGATAAGAAGATGCA+GGG - chr5_3:44201235-44201254 None:intergenic 35.0%
ATATGGTGATAAGAAGATGC+AGG - chr5_3:44201236-44201255 None:intergenic 35.0%
! TTGCTTTCTCAGTTTTTGTG+TGG + chr5_3:44201292-44201311 Msa0813420:CDS 35.0%
! GTTTTTGTGTGGAATGTCTA+CGG + chr5_3:44201303-44201322 Msa0813420:CDS 35.0%
! TTTTTGTGTGGAATGTCTAC+GGG + chr5_3:44201304-44201323 Msa0813420:CDS 35.0%
TGTGTATACAGCTCTTCATT+AGG + chr5_3:44201422-44201441 Msa0813420:intron 35.0%
TATACAGCTCTTCATTAGGA+AGG + chr5_3:44201426-44201445 Msa0813420:intron 35.0%
TGTTTGTGTCAGATATGTTG+TGG + chr5_3:44201498-44201517 Msa0813420:intron 35.0%
CTTGATCCATTAACATTGTC+CGG - chr5_3:44201553-44201572 None:intergenic 35.0%
TGAACCACAGAAACTATAAG+AGG + chr5_3:44201615-44201634 Msa0813420:CDS 35.0%
ATCACCTCTTATAGTTTCTG+TGG - chr5_3:44201622-44201641 None:intergenic 35.0%
TATGGATATTGTTGAATGCG+AGG + chr5_3:44201804-44201823 Msa0813420:intron 35.0%
CCACGAATATTCAAAAACTC+AGG - chr5_3:44201871-44201890 None:intergenic 35.0%
! CCTGAGTTTTTGAATATTCG+TGG + chr5_3:44201868-44201887 Msa0813420:intron 35.0%
!! CGTTGATGTTGGTATTGATA+TGG + chr5_3:44202048-44202067 Msa0813420:intron 35.0%
AAGGGCCATTTCATAAATGA+AGG - chr5_3:44202186-44202205 None:intergenic 35.0%
AGCAAAGAGCTATCAGTTAA+AGG - chr5_3:44202335-44202354 None:intergenic 35.0%
TTTAACTGATAGCTCTTTGC+TGG + chr5_3:44202334-44202353 Msa0813420:intron 35.0%
GCTGGTTATGGTTAATTTCA+AGG + chr5_3:44202352-44202371 Msa0813420:intron 35.0%
! TAATCAGACATTTTGTGCCT+TGG + chr5_3:44202949-44202968 Msa0813420:three_prime_UTR 35.0%
TCGTAGCACTAAAATCGTCT+TGG + chr5_3:44201084-44201103 Msa0813420:intron 40.0%
TTTGTGCCGGACAATGTTAA+TGG + chr5_3:44201544-44201563 Msa0813420:CDS 40.0%
CATCTCTTGTGAAACTGAAC+AGG + chr5_3:44201759-44201778 Msa0813420:CDS 40.0%
GTGTCAAATACCTTATGGCA+GGG - chr5_3:44201839-44201858 None:intergenic 40.0%
! GTGAGGTGTCAAATACCTTA+TGG - chr5_3:44201844-44201863 None:intergenic 40.0%
TCAAAAACTCAGGTGAAGTG+AGG - chr5_3:44201861-44201880 None:intergenic 40.0%
AATACCAACATCAACGGAAG+TGG - chr5_3:44202044-44202063 None:intergenic 40.0%
! CCATCACCTGCATATTTTCA+TGG - chr5_3:44202162-44202181 None:intergenic 40.0%
CCATGAAAATATGCAGGTGA+TGG + chr5_3:44202159-44202178 Msa0813420:intron 40.0%
GAAACACCAATAGCCAGAAT+TGG + chr5_3:44202256-44202275 Msa0813420:intron 40.0%
TTTAACAACGTCGCCAATTC+TGG - chr5_3:44202272-44202291 None:intergenic 40.0%
! TGATAGCTCTTTGCTGGTTA+TGG + chr5_3:44202340-44202359 Msa0813420:intron 40.0%
!!! ATACTTTTTCCACCTCCTTG+TGG + chr5_3:44202503-44202522 Msa0813420:intron 40.0%
GGTGAAAAAATGATCGGTGA+AGG - chr5_3:44202565-44202584 None:intergenic 40.0%
TTTGGTCATACATACAGGCA+AGG + chr5_3:44202676-44202695 Msa0813420:intron 40.0%
TTGGTCATACATACAGGCAA+GGG + chr5_3:44202677-44202696 Msa0813420:intron 40.0%
GCAAGGGATTGAATGTACTT+TGG + chr5_3:44202693-44202712 Msa0813420:exon 40.0%
ATTAAGTTCGGCTCATGTGT+TGG - chr5_3:44202762-44202781 None:intergenic 40.0%
TATAGCTTCTCCTAAGCCTT+AGG + chr5_3:44202814-44202833 Msa0813420:three_prime_UTR 40.0%
CAATCAAGGACAATCTCCTA+AGG - chr5_3:44202833-44202852 None:intergenic 40.0%
CCAGCAAAAAACTACACTAG+AGG - chr5_3:44202921-44202940 None:intergenic 40.0%
!!! CCTCTAGTGTAGTTTTTTGC+TGG + chr5_3:44202918-44202937 Msa0813420:three_prime_UTR 40.0%
! GACATTGTTTGGAACAACCA+AGG - chr5_3:44202969-44202988 None:intergenic 40.0%
TCGTAGCACTAAAATCGTCT+TGG + chr5_3:44201084-44201103 Msa0813420:intron 40.0%
TTTGTGCCGGACAATGTTAA+TGG + chr5_3:44201544-44201563 Msa0813420:CDS 40.0%
CATCTCTTGTGAAACTGAAC+AGG + chr5_3:44201759-44201778 Msa0813420:CDS 40.0%
GTGTCAAATACCTTATGGCA+GGG - chr5_3:44201839-44201858 None:intergenic 40.0%
! GTGAGGTGTCAAATACCTTA+TGG - chr5_3:44201844-44201863 None:intergenic 40.0%
TCAAAAACTCAGGTGAAGTG+AGG - chr5_3:44201861-44201880 None:intergenic 40.0%
AATACCAACATCAACGGAAG+TGG - chr5_3:44202044-44202063 None:intergenic 40.0%
! CCATCACCTGCATATTTTCA+TGG - chr5_3:44202162-44202181 None:intergenic 40.0%
CCATGAAAATATGCAGGTGA+TGG + chr5_3:44202159-44202178 Msa0813420:intron 40.0%
GAAACACCAATAGCCAGAAT+TGG + chr5_3:44202256-44202275 Msa0813420:intron 40.0%
TTTAACAACGTCGCCAATTC+TGG - chr5_3:44202272-44202291 None:intergenic 40.0%
! TGATAGCTCTTTGCTGGTTA+TGG + chr5_3:44202340-44202359 Msa0813420:intron 40.0%
!!! ATACTTTTTCCACCTCCTTG+TGG + chr5_3:44202503-44202522 Msa0813420:intron 40.0%
GGTGAAAAAATGATCGGTGA+AGG - chr5_3:44202565-44202584 None:intergenic 40.0%
TTTGGTCATACATACAGGCA+AGG + chr5_3:44202676-44202695 Msa0813420:intron 40.0%
TTGGTCATACATACAGGCAA+GGG + chr5_3:44202677-44202696 Msa0813420:intron 40.0%
GCAAGGGATTGAATGTACTT+TGG + chr5_3:44202693-44202712 Msa0813420:exon 40.0%
ATTAAGTTCGGCTCATGTGT+TGG - chr5_3:44202762-44202781 None:intergenic 40.0%
TATAGCTTCTCCTAAGCCTT+AGG + chr5_3:44202814-44202833 Msa0813420:three_prime_UTR 40.0%
CAATCAAGGACAATCTCCTA+AGG - chr5_3:44202833-44202852 None:intergenic 40.0%
CCAGCAAAAAACTACACTAG+AGG - chr5_3:44202921-44202940 None:intergenic 40.0%
!!! CCTCTAGTGTAGTTTTTTGC+TGG + chr5_3:44202918-44202937 Msa0813420:three_prime_UTR 40.0%
! GACATTGTTTGGAACAACCA+AGG - chr5_3:44202969-44202988 None:intergenic 40.0%
TCCTGATAGAGTTCACTCCT+TGG + chr5_3:44200781-44200800 Msa0813420:CDS 45.0%
!! CTTGGCTTTGCTAAACGCAA+CGG + chr5_3:44200799-44200818 Msa0813420:CDS 45.0%
!! TTGGCTTTGCTAAACGCAAC+GGG + chr5_3:44200800-44200819 Msa0813420:CDS 45.0%
CAAACTTAGTTCAGGACGAG+TGG + chr5_3:44200977-44200996 Msa0813420:intron 45.0%
!!! GGACGAGTGGTACTGATTTT+AGG + chr5_3:44200990-44201009 Msa0813420:intron 45.0%
CTTGGTTCAAGCTATTAGCC+AGG + chr5_3:44201399-44201418 Msa0813420:intron 45.0%
TGAAGAGCTGTATACACACC+TGG - chr5_3:44201420-44201439 None:intergenic 45.0%
AACATTGTCCGGCACAAACA+TGG - chr5_3:44201542-44201561 None:intergenic 45.0%
CTTGCTTTCAGGAAAAGCAG+TGG - chr5_3:44201582-44201601 None:intergenic 45.0%
!! CTGCTTTTCCTGAAAGCAAG+AGG + chr5_3:44201582-44201601 Msa0813420:CDS 45.0%
! TGAAGCATCCTCTTGCTTTC+AGG - chr5_3:44201593-44201612 None:intergenic 45.0%
GAATTGAGATCCCTGCCATA+AGG + chr5_3:44201826-44201845 Msa0813420:intron 45.0%
GGTGTCAAATACCTTATGGC+AGG - chr5_3:44201840-44201859 None:intergenic 45.0%
ATCTCCACTTCCGTTGATGT+TGG + chr5_3:44202037-44202056 Msa0813420:intron 45.0%
TCGGAGTTTCATGTTCGATC+AGG + chr5_3:44202232-44202251 Msa0813420:intron 45.0%
CGGAGTTTCATGTTCGATCA+GGG + chr5_3:44202233-44202252 Msa0813420:intron 45.0%
GGAGTTTCATGTTCGATCAG+GGG + chr5_3:44202234-44202253 Msa0813420:intron 45.0%
! TTTTTCCACCTCCTTGTGGT+TGG + chr5_3:44202507-44202526 Msa0813420:intron 45.0%
GAAAACCAACTCCAACCACA+AGG - chr5_3:44202521-44202540 None:intergenic 45.0%
!!! TGTGGTTGGAGTTGGTTTTC+AGG + chr5_3:44202521-44202540 Msa0813420:intron 45.0%
CCATTGTTCACTGCCTTCTT+TGG - chr5_3:44202735-44202754 None:intergenic 45.0%
CCAAAGAAGGCAGTGAACAA+TGG + chr5_3:44202732-44202751 Msa0813420:three_prime_UTR 45.0%
AGGACAATCTCCTAAGGCTT+AGG - chr5_3:44202827-44202846 None:intergenic 45.0%
TCCTGATAGAGTTCACTCCT+TGG + chr5_3:44200781-44200800 Msa0813420:CDS 45.0%
!! CTTGGCTTTGCTAAACGCAA+CGG + chr5_3:44200799-44200818 Msa0813420:CDS 45.0%
!! TTGGCTTTGCTAAACGCAAC+GGG + chr5_3:44200800-44200819 Msa0813420:CDS 45.0%
CAAACTTAGTTCAGGACGAG+TGG + chr5_3:44200977-44200996 Msa0813420:intron 45.0%
!!! GGACGAGTGGTACTGATTTT+AGG + chr5_3:44200990-44201009 Msa0813420:intron 45.0%
CTTGGTTCAAGCTATTAGCC+AGG + chr5_3:44201399-44201418 Msa0813420:intron 45.0%
TGAAGAGCTGTATACACACC+TGG - chr5_3:44201420-44201439 None:intergenic 45.0%
AACATTGTCCGGCACAAACA+TGG - chr5_3:44201542-44201561 None:intergenic 45.0%
CTTGCTTTCAGGAAAAGCAG+TGG - chr5_3:44201582-44201601 None:intergenic 45.0%
!! CTGCTTTTCCTGAAAGCAAG+AGG + chr5_3:44201582-44201601 Msa0813420:CDS 45.0%
! TGAAGCATCCTCTTGCTTTC+AGG - chr5_3:44201593-44201612 None:intergenic 45.0%
GAATTGAGATCCCTGCCATA+AGG + chr5_3:44201826-44201845 Msa0813420:intron 45.0%
GGTGTCAAATACCTTATGGC+AGG - chr5_3:44201840-44201859 None:intergenic 45.0%
ATCTCCACTTCCGTTGATGT+TGG + chr5_3:44202037-44202056 Msa0813420:intron 45.0%
TCGGAGTTTCATGTTCGATC+AGG + chr5_3:44202232-44202251 Msa0813420:intron 45.0%
CGGAGTTTCATGTTCGATCA+GGG + chr5_3:44202233-44202252 Msa0813420:intron 45.0%
GGAGTTTCATGTTCGATCAG+GGG + chr5_3:44202234-44202253 Msa0813420:intron 45.0%
! TTTTTCCACCTCCTTGTGGT+TGG + chr5_3:44202507-44202526 Msa0813420:intron 45.0%
GAAAACCAACTCCAACCACA+AGG - chr5_3:44202521-44202540 None:intergenic 45.0%
!!! TGTGGTTGGAGTTGGTTTTC+AGG + chr5_3:44202521-44202540 Msa0813420:intron 45.0%
CCATTGTTCACTGCCTTCTT+TGG - chr5_3:44202735-44202754 None:intergenic 45.0%
CCAAAGAAGGCAGTGAACAA+TGG + chr5_3:44202732-44202751 Msa0813420:three_prime_UTR 45.0%
AGGACAATCTCCTAAGGCTT+AGG - chr5_3:44202827-44202846 None:intergenic 45.0%
!!! AATACATTTTTTATTTAAAA+GGG + chr5_3:44202989-44203008 Msa0813420:three_prime_UTR 5.0%
!!! AATACATTTTTTATTTAAAA+GGG + chr5_3:44202989-44203008 Msa0813420:three_prime_UTR 5.0%
GCCAAGGAGTGAACTCTATC+AGG - chr5_3:44200785-44200804 None:intergenic 50.0%
CGTTGCGTTTAGCAAAGCCA+AGG - chr5_3:44200801-44200820 None:intergenic 50.0%
AGTCCCAACCATGTTTGTGC+CGG + chr5_3:44201531-44201550 Msa0813420:CDS 50.0%
TGTCCGGCACAAACATGGTT+GGG - chr5_3:44201537-44201556 None:intergenic 50.0%
TTGTCCGGCACAAACATGGT+TGG - chr5_3:44201538-44201557 None:intergenic 50.0%
TCGTGGTTGCCCAATATTCG+TGG + chr5_3:44201885-44201904 Msa0813420:intron 50.0%
! ACGTCGCCAATTCTGGCTAT+TGG - chr5_3:44202265-44202284 None:intergenic 50.0%
AACCAACTCCAACCACAAGG+AGG - chr5_3:44202518-44202537 None:intergenic 50.0%
GCCAAGGAGTGAACTCTATC+AGG - chr5_3:44200785-44200804 None:intergenic 50.0%
CGTTGCGTTTAGCAAAGCCA+AGG - chr5_3:44200801-44200820 None:intergenic 50.0%
AGTCCCAACCATGTTTGTGC+CGG + chr5_3:44201531-44201550 Msa0813420:CDS 50.0%
TGTCCGGCACAAACATGGTT+GGG - chr5_3:44201537-44201556 None:intergenic 50.0%
TTGTCCGGCACAAACATGGT+TGG - chr5_3:44201538-44201557 None:intergenic 50.0%
TCGTGGTTGCCCAATATTCG+TGG + chr5_3:44201885-44201904 Msa0813420:intron 50.0%
! ACGTCGCCAATTCTGGCTAT+TGG - chr5_3:44202265-44202284 None:intergenic 50.0%
AACCAACTCCAACCACAAGG+AGG - chr5_3:44202518-44202537 None:intergenic 50.0%
GCTTTGCTAAACGCAACGGG+TGG + chr5_3:44200803-44200822 Msa0813420:CDS 55.0%
CAACTCCAACCACAAGGAGG+TGG - chr5_3:44202515-44202534 None:intergenic 55.0%
CACCTCCTTGTGGTTGGAGT+TGG + chr5_3:44202513-44202532 Msa0813420:intron 55.0%
GCTTTGCTAAACGCAACGGG+TGG + chr5_3:44200803-44200822 Msa0813420:CDS 55.0%
CAACTCCAACCACAAGGAGG+TGG - chr5_3:44202515-44202534 None:intergenic 55.0%
CACCTCCTTGTGGTTGGAGT+TGG + chr5_3:44202513-44202532 Msa0813420:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 44200685 44203114 44200685 ID=Msa0813420;Name=Msa0813420
chr5_3 mRNA 44200685 44203114 44200685 ID=Msa0813420-mRNA-1;Parent=Msa0813420;Name=Msa0813420-mRNA-1;_AED=0.30;_eAED=0.34;_QI=67|0|0.5|1|0.2|0.33|6|410|111
chr5_3 exon 44200685 44200847 44200685 ID=Msa0813420-mRNA-1:exon:13653;Parent=Msa0813420-mRNA-1
chr5_3 exon 44200991 44200998 44200991 ID=Msa0813420-mRNA-1:exon:13654;Parent=Msa0813420-mRNA-1
chr5_3 exon 44201285 44201325 44201285 ID=Msa0813420-mRNA-1:exon:13655;Parent=Msa0813420-mRNA-1
chr5_3 exon 44201510 44201636 44201510 ID=Msa0813420-mRNA-1:exon:13656;Parent=Msa0813420-mRNA-1
chr5_3 exon 44201729 44201780 44201729 ID=Msa0813420-mRNA-1:exon:13657;Parent=Msa0813420-mRNA-1
chr5_3 exon 44202693 44203114 44202693 ID=Msa0813420-mRNA-1:exon:13658;Parent=Msa0813420-mRNA-1
chr5_3 five_prime_UTR 44200685 44200751 44200685 ID=Msa0813420-mRNA-1:five_prime_utr;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44200752 44200847 44200752 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44200991 44200998 44200991 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44201285 44201325 44201285 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44201510 44201636 44201510 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44201729 44201780 44201729 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 CDS 44202693 44202704 44202693 ID=Msa0813420-mRNA-1:cds;Parent=Msa0813420-mRNA-1
chr5_3 three_prime_UTR 44202705 44203114 44202705 ID=Msa0813420-mRNA-1:three_prime_utr;Parent=Msa0813420-mRNA-1
Gene Sequence

>Msa0813420

ATGATAGCTTGTAAAATAGCAGCAATGGTTCCTGATAGAGTTCACTCCTTGGCTTTGCTAAACGCAACGGGTGGAGATTTTCAATGTTTTCCAGAGGACGAGTGGAATCACTTGCTTTCTCAGTTTTTGTGTGGAATGTCTACGGATATGTTGTGGAATGCTGCTGAGTCCCAACCATGTTTGTGCCGGACAATGTTAATGGATCAAGTTGTGTCCACTGCTTTTCCTGAAAGCAAGAGGATGCTTCAGCTGAACCACAGAAACTATAAGAGATTAGCATCAGTCATGCAACTTACAGAGAGCATCTCTTGTGAAACTGAACAGGCAAGGGATTGA
Protein sequence

>Msa0813420

MIACKIAAMVPDRVHSLALLNATGGDFQCFPEDEWNHLLSQFLCGMSTDMLWNAAESQPCLCRTMLMDQVVSTAFPESKRMLQLNHRNYKRLASVMQLTESISCETEQARD*