Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0813850 | Msa0872150 | 0.800446 | 1.435993e-48 | -8.615850e-47 |
| Msa0813850 | Msa0981110 | 0.862033 | 6.748422e-64 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCAGCTCATTACAGATTT+TGG | 0.154313 | 5_3:-45147880 | Msa0813850:intron |
| TGATTGTTAGGTCCTGGGTT+AGG | 0.326219 | 5_3:+45148530 | None:intergenic |
| ATCCAATCCTCGACTCAATT+CGG | 0.383316 | 5_3:-45148372 | Msa0813850:intron |
| TCGAGGATTGGATTCGTATT+CGG | 0.383722 | 5_3:+45148382 | None:intergenic |
| GAGAGAGATGCATGATTGTT+AGG | 0.387754 | 5_3:+45148518 | None:intergenic |
| AAGAGAAACAACACCGGAAC+TGG | 0.390313 | 5_3:-45148468 | Msa0813850:CDS |
| ATCTGCTGCGGTGCCAGTTC+CGG | 0.402583 | 5_3:+45148455 | None:intergenic |
| GCAGGATGAAGGAGAGAGAA+GGG | 0.410575 | 5_3:-45148190 | Msa0813850:intron |
| GATTGTTAGGTCCTGGGTTA+GGG | 0.434444 | 5_3:+45148531 | None:intergenic |
| GAGAGAAGGGTAGGGCAAGA+AGG | 0.440573 | 5_3:-45148177 | Msa0813850:CDS |
| GATGCATGATTGTTAGGTCC+TGG | 0.445334 | 5_3:+45148524 | None:intergenic |
| TACCGAATTGAGTCGAGGAT+TGG | 0.448746 | 5_3:+45148370 | None:intergenic |
| TTTATGGTATTGCAGGATGA+AGG | 0.487642 | 5_3:-45148201 | Msa0813850:intron |
| TAATCTATTCAGAGATCAAT+CGG | 0.497849 | 5_3:-45148426 | Msa0813850:CDS |
| ATGCATGATTGTTAGGTCCT+GGG | 0.513842 | 5_3:+45148525 | None:intergenic |
| TGCAGGATGAAGGAGAGAGA+AGG | 0.521958 | 5_3:-45148191 | Msa0813850:intron |
| GATGAAGGAGAGAGAAGGGT+AGG | 0.529746 | 5_3:-45148186 | Msa0813850:CDS |
| ATTCAGAGATCAATCGGAAG+AGG | 0.569947 | 5_3:-45148420 | Msa0813850:CDS |
| GAAGAAGAAGTAGAGAGTGA+AGG | 0.574931 | 5_3:-45148135 | Msa0813850:intron |
| ATGAAGGAGAGAGAAGGGTA+GGG | 0.580265 | 5_3:-45148185 | Msa0813850:CDS |
| AGGGTAGGGCAAGAAGGTGA+AGG | 0.585834 | 5_3:-45148171 | Msa0813850:CDS |
| GGGCAAGAAGGTGAAGGACA+AGG | 0.613410 | 5_3:-45148165 | Msa0813850:CDS |
| TTTAGAAACATCAATTGCGG+TGG | 0.636797 | 5_3:-45147822 | None:intergenic |
| CTGTTCGATTTCCCTAACCC+AGG | 0.641307 | 5_3:-45148542 | Msa0813850:CDS |
| AGCTTTAGAAACATCAATTG+CGG | 0.642741 | 5_3:-45147825 | Msa0813850:three_prime_UTR |
| AATCTTACCGAATTGAGTCG+AGG | 0.654505 | 5_3:+45148365 | None:intergenic |
| AGATTATGCATCATCTGCTG+CGG | 0.660899 | 5_3:+45148443 | None:intergenic |
| CGGGAGAAGAGAAACAACAC+CGG | 0.689084 | 5_3:-45148474 | Msa0813850:CDS |
| GGGTTAGGGAAATCGAACAG+AGG | 0.744365 | 5_3:+45148545 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCATATTTGAAATTTGAAA+TGG | - | chr5_3:45148139-45148158 | Msa0813850:CDS | 15.0% |
| !!! | AACTAAAACAAAATTTTATC+AGG | + | chr5_3:45148328-45148347 | None:intergenic | 15.0% |
| !!! | AATTTGAAATGGTGAATTTA+TGG | - | chr5_3:45148150-45148169 | Msa0813850:CDS | 20.0% |
| !! | GAACAAAACAATTATAGAAT+GGG | + | chr5_3:45148357-45148376 | None:intergenic | 20.0% |
| !! | AGAACAAAACAATTATAGAA+TGG | + | chr5_3:45148358-45148377 | None:intergenic | 20.0% |
| !! | AATTAACCAAAATCTGTAAA+TGG | + | chr5_3:45148469-45148488 | None:intergenic | 20.0% |
| !! | TTCAAAAAATATGAAACTGT+AGG | - | chr5_3:45148511-45148530 | Msa0813850:CDS | 20.0% |
| !!! | ACACATTTTTTCTGTTCAAT+CGG | - | chr5_3:45147873-45147892 | Msa0813850:CDS | 25.0% |
| ! | TAATCTATTCAGAGATCAAT+CGG | - | chr5_3:45147941-45147960 | Msa0813850:intron | 25.0% |
| ! | ACAAAATTTGAATGAAGCTA+GGG | + | chr5_3:45148068-45148087 | None:intergenic | 25.0% |
| ! | TATTCGTTTGTTTATGCATT+TGG | - | chr5_3:45148113-45148132 | Msa0813850:intron | 25.0% |
| ! | ATATAGAAGTAGCTTCAAAA+TGG | - | chr5_3:45148418-45148437 | Msa0813850:CDS | 25.0% |
| !!! | CACATTTTTTCTGTTCAATC+GGG | - | chr5_3:45147874-45147893 | Msa0813850:CDS | 30.0% |
| CACAAAATTTGAATGAAGCT+AGG | + | chr5_3:45148069-45148088 | None:intergenic | 30.0% | |
| ! | GCTTAACCATTTACAGATTT+TGG | - | chr5_3:45148460-45148479 | Msa0813850:CDS | 30.0% |
| TGGTGAATTTATGGTATTGC+AGG | - | chr5_3:45148159-45148178 | Msa0813850:CDS | 35.0% | |
| TTTATGGTATTGCAGGATGA+AGG | - | chr5_3:45148166-45148185 | Msa0813850:CDS | 35.0% | |
| ! | TTGCAGCTCATTACAGATTT+TGG | - | chr5_3:45148487-45148506 | Msa0813850:CDS | 35.0% |
| ATGCATGATTGTTAGGTCCT+GGG | + | chr5_3:45147845-45147864 | None:intergenic | 40.0% | |
| GAGAGAGATGCATGATTGTT+AGG | + | chr5_3:45147852-45147871 | None:intergenic | 40.0% | |
| AGATTATGCATCATCTGCTG+CGG | + | chr5_3:45147927-45147946 | None:intergenic | 40.0% | |
| ATTCAGAGATCAATCGGAAG+AGG | - | chr5_3:45147947-45147966 | Msa0813850:intron | 40.0% | |
| TCGAGGATTGGATTCGTATT+CGG | + | chr5_3:45147988-45148007 | None:intergenic | 40.0% | |
| ATCCAATCCTCGACTCAATT+CGG | - | chr5_3:45147995-45148014 | Msa0813850:intron | 40.0% | |
| AATCTTACCGAATTGAGTCG+AGG | + | chr5_3:45148005-45148024 | None:intergenic | 40.0% | |
| GAAGAAGAAGTAGAGAGTGA+AGG | - | chr5_3:45148232-45148251 | Msa0813850:intron | 40.0% | |
| GATTGTTAGGTCCTGGGTTA+GGG | + | chr5_3:45147839-45147858 | None:intergenic | 45.0% | |
| TGATTGTTAGGTCCTGGGTT+AGG | + | chr5_3:45147840-45147859 | None:intergenic | 45.0% | |
| GATGCATGATTGTTAGGTCC+TGG | + | chr5_3:45147846-45147865 | None:intergenic | 45.0% | |
| AAGAGAAACAACACCGGAAC+TGG | - | chr5_3:45147899-45147918 | Msa0813850:intron | 45.0% | |
| TACCGAATTGAGTCGAGGAT+TGG | + | chr5_3:45148000-45148019 | None:intergenic | 45.0% | |
| ATGAAGGAGAGAGAAGGGTA+GGG | - | chr5_3:45148182-45148201 | Msa0813850:CDS | 45.0% | |
| AAGTAGAGAGTGAAGGTGAG+AGG | - | chr5_3:45148239-45148258 | Msa0813850:intron | 45.0% | |
| AGTAGAGAGTGAAGGTGAGA+GGG | - | chr5_3:45148240-45148259 | Msa0813850:intron | 45.0% | |
| CTGTTCGATTTCCCTAACCC+AGG | - | chr5_3:45147825-45147844 | Msa0813850:three_prime_UTR | 50.0% | |
| CGGGAGAAGAGAAACAACAC+CGG | - | chr5_3:45147893-45147912 | Msa0813850:intron | 50.0% | |
| TGCAGGATGAAGGAGAGAGA+AGG | - | chr5_3:45148176-45148195 | Msa0813850:CDS | 50.0% | |
| GCAGGATGAAGGAGAGAGAA+GGG | - | chr5_3:45148177-45148196 | Msa0813850:CDS | 50.0% | |
| GATGAAGGAGAGAGAAGGGT+AGG | - | chr5_3:45148181-45148200 | Msa0813850:CDS | 50.0% | |
| GAGAGAAGGGTAGGGCAAGA+AGG | - | chr5_3:45148190-45148209 | Msa0813850:intron | 55.0% | |
| AGGGTAGGGCAAGAAGGTGA+AGG | - | chr5_3:45148196-45148215 | Msa0813850:intron | 55.0% | |
| GGGCAAGAAGGTGAAGGACA+AGG | - | chr5_3:45148202-45148221 | Msa0813850:intron | 55.0% | |
| AGAGAGTGAAGGTGAGAGGG+AGG | - | chr5_3:45148243-45148262 | Msa0813850:intron | 55.0% | |
| ! | ATCTGCTGCGGTGCCAGTTC+CGG | + | chr5_3:45147915-45147934 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 45147825 | 45148564 | 45147825 | ID=Msa0813850;Name=Msa0813850 |
| chr5_3 | mRNA | 45147825 | 45148564 | 45147825 | ID=Msa0813850-mRNA-1;Parent=Msa0813850;Name=Msa0813850-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0.66|1|0.5|0.33|3|40|98 |
| chr5_3 | exon | 45147825 | 45147896 | 45147825 | ID=Msa0813850-mRNA-1:exon:13858;Parent=Msa0813850-mRNA-1 |
| chr5_3 | exon | 45148136 | 45148208 | 45148136 | ID=Msa0813850-mRNA-1:exon:13857;Parent=Msa0813850-mRNA-1 |
| chr5_3 | exon | 45148373 | 45148564 | 45148373 | ID=Msa0813850-mRNA-1:exon:13856;Parent=Msa0813850-mRNA-1 |
| chr5_3 | CDS | 45148373 | 45148564 | 45148373 | ID=Msa0813850-mRNA-1:cds;Parent=Msa0813850-mRNA-1 |
| chr5_3 | CDS | 45148136 | 45148208 | 45148136 | ID=Msa0813850-mRNA-1:cds;Parent=Msa0813850-mRNA-1 |
| chr5_3 | CDS | 45147865 | 45147896 | 45147865 | ID=Msa0813850-mRNA-1:cds;Parent=Msa0813850-mRNA-1 |
| chr5_3 | three_prime_UTR | 45147825 | 45147864 | 45147825 | ID=Msa0813850-mRNA-1:three_prime_utr;Parent=Msa0813850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
