Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0813850 | Msa0981110 | 0.862033 | 6.748422e-64 | -8.615850e-47 |
| Msa0872150 | Msa0981110 | 0.863442 | 2.482371e-64 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCAGCTCATTACAGATTT+TGG | 0.154313 | 6_4:-20272309 | Msa0981110:intron |
| AAGAGAAACCACACCGGAAC+TGG | 0.370310 | 6_4:-20272895 | Msa0981110:five_prime_UTR |
| ATCCAATCCTCGACTCAATT+CGG | 0.383316 | 6_4:-20272799 | Msa0981110:intron |
| TCGAGGATTGGATTCGTATT+CGG | 0.383722 | 6_4:+20272809 | None:intergenic |
| ATCTGCTGCGGTGCCAGTTC+CGG | 0.402583 | 6_4:+20272882 | None:intergenic |
| GCAGGATGAAGGAGAGAGAA+GGG | 0.410575 | 6_4:-20272617 | Msa0981110:intron |
| GAGAGAAGGGTAGGGCAAGA+AGG | 0.440573 | 6_4:-20272604 | Msa0981110:CDS |
| TACCGAATTGAGTCGAGGAT+TGG | 0.448746 | 6_4:+20272797 | None:intergenic |
| TTTATGGTATTGCAGGATGA+AGG | 0.487642 | 6_4:-20272628 | Msa0981110:intron |
| TAATCTATTCAGAGATCAAT+CGG | 0.497849 | 6_4:-20272853 | Msa0981110:CDS |
| TGCAGGATGAAGGAGAGAGA+AGG | 0.521958 | 6_4:-20272618 | Msa0981110:intron |
| GATGAAGGAGAGAGAAGGGT+AGG | 0.529746 | 6_4:-20272613 | Msa0981110:CDS |
| ATTCAGAGATCAATCGGAAG+AGG | 0.569947 | 6_4:-20272847 | Msa0981110:CDS |
| GAAGAAGAAGTAGAGAGTGA+AGG | 0.574931 | 6_4:-20272562 | Msa0981110:intron |
| ATGAAGGAGAGAGAAGGGTA+GGG | 0.580265 | 6_4:-20272612 | Msa0981110:CDS |
| AGGGTAGGGCAAGAAGGTGA+AGG | 0.585834 | 6_4:-20272598 | Msa0981110:CDS |
| GGGCAAGAAGGTGAAGGACA+AGG | 0.613410 | 6_4:-20272592 | Msa0981110:CDS |
| TTTAGAAACATCAATTGCGG+TGG | 0.636797 | 6_4:-20272251 | None:intergenic |
| AGCTTTAGAAACATCAATTG+CGG | 0.642741 | 6_4:-20272254 | Msa0981110:three_prime_UTR |
| AATCTTACCGAATTGAGTCG+AGG | 0.654505 | 6_4:+20272792 | None:intergenic |
| CTGCGGTGCCAGTTCCGGTG+TGG | 0.658353 | 6_4:+20272887 | None:intergenic |
| AGATTATGCATCATCTGCTG+CGG | 0.660899 | 6_4:+20272870 | None:intergenic |
| GGGGAGAAGAGAAACCACAC+CGG | 0.748418 | 6_4:-20272901 | Msa0981110:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAAAACAAAATTTTATC+AGG | + | chr6_4:20272720-20272739 | None:intergenic | 10.0% |
| !!! | ATCATATTTGAAATTTGAAA+TGG | - | chr6_4:20272530-20272549 | Msa0981110:intron | 15.0% |
| !! | AATTAACCAAAATTTGTAAA+TGG | + | chr6_4:20272858-20272877 | None:intergenic | 15.0% |
| !!! | AATTTGAAATGGTGAATTTA+TGG | - | chr6_4:20272541-20272560 | Msa0981110:intron | 20.0% |
| !! | GAACAAAACAATTATAGAAT+GGG | + | chr6_4:20272749-20272768 | None:intergenic | 20.0% |
| !! | AGAACAAAACAATTATAGAA+TGG | + | chr6_4:20272750-20272769 | None:intergenic | 20.0% |
| !! | TTCAAAAAATATGAAACTGT+AGG | - | chr6_4:20272900-20272919 | Msa0981110:five_prime_UTR | 20.0% |
| !!! | ACACATTTTTTCTGTTCAAT+AGG | - | chr6_4:20272263-20272282 | Msa0981110:three_prime_UTR | 25.0% |
| !!! | CACATTTTTTCTGTTCAATA+GGG | - | chr6_4:20272264-20272283 | Msa0981110:three_prime_UTR | 25.0% |
| !!! | ACATTTTTTCTGTTCAATAG+GGG | - | chr6_4:20272265-20272284 | Msa0981110:three_prime_UTR | 25.0% |
| ! | TAATCTATTCAGAGATCAAT+CGG | - | chr6_4:20272332-20272351 | Msa0981110:intron | 25.0% |
| ! | ACAAAATTTGAATGAAGCTA+GGG | + | chr6_4:20272459-20272478 | None:intergenic | 25.0% |
| ! | TATTCGTTTGTTTATGCATT+TGG | - | chr6_4:20272504-20272523 | Msa0981110:intron | 25.0% |
| ! | ATATAGAAGTAGCTTCAAAA+TGG | - | chr6_4:20272807-20272826 | Msa0981110:CDS | 25.0% |
| !! | GCTTAACCATTTACAAATTT+TGG | - | chr6_4:20272849-20272868 | Msa0981110:CDS | 25.0% |
| CACAAAATTTGAATGAAGCT+AGG | + | chr6_4:20272460-20272479 | None:intergenic | 30.0% | |
| TGGTGAATTTATGGTATTGC+AGG | - | chr6_4:20272550-20272569 | Msa0981110:intron | 35.0% | |
| TTTATGGTATTGCAGGATGA+AGG | - | chr6_4:20272557-20272576 | Msa0981110:intron | 35.0% | |
| ! | TTGCAGCTCATTACAGATTT+TGG | - | chr6_4:20272876-20272895 | Msa0981110:exon | 35.0% |
| AGATTATGCATCATCTGCTG+CGG | + | chr6_4:20272318-20272337 | None:intergenic | 40.0% | |
| ATTCAGAGATCAATCGGAAG+AGG | - | chr6_4:20272338-20272357 | Msa0981110:intron | 40.0% | |
| TCGAGGATTGGATTCGTATT+CGG | + | chr6_4:20272379-20272398 | None:intergenic | 40.0% | |
| ATCCAATCCTCGACTCAATT+CGG | - | chr6_4:20272386-20272405 | Msa0981110:intron | 40.0% | |
| AATCTTACCGAATTGAGTCG+AGG | + | chr6_4:20272396-20272415 | None:intergenic | 40.0% | |
| GAAGAAGAAGTAGAGAGTGA+AGG | - | chr6_4:20272623-20272642 | Msa0981110:intron | 40.0% | |
| TACCGAATTGAGTCGAGGAT+TGG | + | chr6_4:20272391-20272410 | None:intergenic | 45.0% | |
| ATGAAGGAGAGAGAAGGGTA+GGG | - | chr6_4:20272573-20272592 | Msa0981110:CDS | 45.0% | |
| AAGTAGAGAGTGAAGGTGAG+AGG | - | chr6_4:20272630-20272649 | Msa0981110:intron | 45.0% | |
| AGTAGAGAGTGAAGGTGAGA+GGG | - | chr6_4:20272631-20272650 | Msa0981110:intron | 45.0% | |
| AAGAGAAACCACACCGGAAC+TGG | - | chr6_4:20272290-20272309 | Msa0981110:exon | 50.0% | |
| TGCAGGATGAAGGAGAGAGA+AGG | - | chr6_4:20272567-20272586 | Msa0981110:CDS | 50.0% | |
| GCAGGATGAAGGAGAGAGAA+GGG | - | chr6_4:20272568-20272587 | Msa0981110:CDS | 50.0% | |
| GATGAAGGAGAGAGAAGGGT+AGG | - | chr6_4:20272572-20272591 | Msa0981110:CDS | 50.0% | |
| GGGGAGAAGAGAAACCACAC+CGG | - | chr6_4:20272284-20272303 | Msa0981110:exon | 55.0% | |
| GAGAGAAGGGTAGGGCAAGA+AGG | - | chr6_4:20272581-20272600 | Msa0981110:CDS | 55.0% | |
| AGGGTAGGGCAAGAAGGTGA+AGG | - | chr6_4:20272587-20272606 | Msa0981110:CDS | 55.0% | |
| GGGCAAGAAGGTGAAGGACA+AGG | - | chr6_4:20272593-20272612 | Msa0981110:CDS | 55.0% | |
| AGAGAGTGAAGGTGAGAGGG+AGG | - | chr6_4:20272634-20272653 | Msa0981110:intron | 55.0% | |
| ! | ATCTGCTGCGGTGCCAGTTC+CGG | + | chr6_4:20272306-20272325 | None:intergenic | 60.0% |
| ! | CTGCGGTGCCAGTTCCGGTG+TGG | + | chr6_4:20272301-20272320 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 20272254 | 20272953 | 20272254 | ID=Msa0981110;Name=Msa0981110 |
| chr6_4 | mRNA | 20272254 | 20272953 | 20272254 | ID=Msa0981110-mRNA-1;Parent=Msa0981110;Name=Msa0981110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=70|1|1|1|0.5|0.66|3|40|62 |
| chr6_4 | exon | 20272254 | 20272325 | 20272254 | ID=Msa0981110-mRNA-1:exon:6192;Parent=Msa0981110-mRNA-1 |
| chr6_4 | exon | 20272563 | 20272635 | 20272563 | ID=Msa0981110-mRNA-1:exon:6191;Parent=Msa0981110-mRNA-1 |
| chr6_4 | exon | 20272800 | 20272953 | 20272800 | ID=Msa0981110-mRNA-1:exon:6190;Parent=Msa0981110-mRNA-1 |
| chr6_4 | five_prime_UTR | 20272884 | 20272953 | 20272884 | ID=Msa0981110-mRNA-1:five_prime_utr;Parent=Msa0981110-mRNA-1 |
| chr6_4 | CDS | 20272800 | 20272883 | 20272800 | ID=Msa0981110-mRNA-1:cds;Parent=Msa0981110-mRNA-1 |
| chr6_4 | CDS | 20272563 | 20272635 | 20272563 | ID=Msa0981110-mRNA-1:cds;Parent=Msa0981110-mRNA-1 |
| chr6_4 | CDS | 20272294 | 20272325 | 20272294 | ID=Msa0981110-mRNA-1:cds;Parent=Msa0981110-mRNA-1 |
| chr6_4 | three_prime_UTR | 20272254 | 20272293 | 20272254 | ID=Msa0981110-mRNA-1:three_prime_utr;Parent=Msa0981110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
