AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0817030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817030 MtrunA17_Chr5g0435861 100.000 131 0 0 1 131 125 255 2.16e-94 272
Msa0817030 MtrunA17_Chr7g0239661 57.692 130 54 1 1 130 125 253 4.71e-48 154
Msa0817030 MtrunA17_Chr4g0001071 45.283 106 55 2 25 129 131 234 1.71e-25 96.3
Msa0817030 MtrunA17_Chr5g0435841 66.250 80 18 3 1 79 1 72 1.54e-24 90.1
Msa0817030 MtrunA17_Chr1g0149801 38.017 121 71 3 11 129 113 231 2.51e-20 82.8
Msa0817030 MtrunA17_Chr5g0444341 34.043 94 55 2 42 129 120 212 1.42e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817030 AT3G55605.1 67.273 110 35 1 22 130 148 257 1.25e-49 159
Msa0817030 AT5G02050.1 61.062 113 44 0 18 130 154 266 7.21e-45 147
Msa0817030 AT5G05990.1 62.264 106 39 1 26 130 153 258 6.33e-44 144
Msa0817030 AT2G39795.1 57.252 131 51 2 1 130 123 249 1.31e-43 143
Msa0817030 AT2G39790.1 43.137 102 55 2 28 129 140 238 5.79e-22 87.8
Msa0817030 AT1G15870.1 39.815 108 60 2 25 130 136 240 3.98e-21 85.5
Msa0817030 AT4G32605.1 35.772 123 70 3 12 129 99 217 6.90e-18 76.6
Msa0817030 AT4G31930.1 38.053 113 66 3 19 129 122 232 1.63e-17 75.9
Msa0817030 AT1G80720.1 35.922 103 64 2 29 130 87 188 2.60e-17 74.3
Msa0817030 AT1G80720.2 35.922 103 64 2 29 130 139 240 6.69e-17 74.3

Find 48 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTATCAACTATCTTAAA+TGG 0.175868 5_3:+51896105 None:intergenic
CTGTGGAAGGTTTCATTCTT+AGG 0.219897 5_3:-51895160 Msa0817030:three_prime_UTR
TCCACAAGTACTTAGAAATA+AGG 0.241404 5_3:-51895341 Msa0817030:CDS
CACATGCCTGATTTGGTTAC+TGG 0.250062 5_3:-51896021 Msa0817030:CDS
AACCTTCCACAGTGATAATA+TGG 0.251439 5_3:+51895171 None:intergenic
AGTGTTATTGTCTGATTTCC+TGG 0.252098 5_3:+51896084 None:intergenic
ATCAGACAATAACACTTAAA+AGG 0.289378 5_3:-51896077 Msa0817030:five_prime_UTR
TAAGAAGGATGGGCCCTCTT+TGG 0.320568 5_3:-51895911 Msa0817030:CDS
TTATCAAGGTGAAGAAATTC+AGG 0.325526 5_3:-51896052 Msa0817030:five_prime_UTR
CCACAAGTACTTAGAAATAA+GGG 0.328236 5_3:-51895340 Msa0817030:CDS
CTGTCGATAACAATCTCATC+TGG 0.355760 5_3:+51895868 None:intergenic
TGAGGTTCACATGCCTGATT+TGG 0.368998 5_3:-51896028 Msa0817030:exon
TAGCACTTATATCCTTAGTA+AGG 0.378658 5_3:-51895047 Msa0817030:three_prime_UTR
AGAAGTACATACTGAAAATC+TGG 0.385204 5_3:+51895793 None:intergenic
CCCTTATTTCTAAGTACTTG+TGG 0.408675 5_3:+51895340 None:intergenic
AACCAGTGTGCCTTTAAAAC+AGG 0.414757 5_3:+51895078 None:intergenic
TTCAGTCAGTGTTTCTAAGA+AGG 0.415683 5_3:-51895926 Msa0817030:CDS
TGCAAGAAATTTGTTGTGCT+TGG 0.436674 5_3:+51895309 None:intergenic
TGAAAATCTGGCCCGTCATA+AGG 0.439441 5_3:+51895805 None:intergenic
CAAGGATAGTAGAGAATACT+TGG 0.453079 5_3:-51895271 Msa0817030:CDS
GATTATCAACTATCTTAAAT+GGG 0.456111 5_3:+51896106 None:intergenic
TCTTCACCAGTAACCAAATC+AGG 0.463223 5_3:+51896015 None:intergenic
TTAGAAACACTGACTGAAAG+TGG 0.463235 5_3:+51895931 None:intergenic
ATTGTGTCATTGTGGTTTAG+TGG 0.464000 5_3:-51895113 Msa0817030:three_prime_UTR
GTCAGTGTTTCTAAGAAGGA+TGG 0.492712 5_3:-51895922 Msa0817030:CDS
TGATCATCAAGATGATGATA+AGG 0.496458 5_3:-51895989 Msa0817030:CDS
ATGTAATAAATCCCTTACTA+AGG 0.497032 5_3:+51895035 None:intergenic
TCCCATATTATCACTGTGGA+AGG 0.503429 5_3:-51895173 Msa0817030:three_prime_UTR
TTTAAGATAGTTGATAATCC+AGG 0.504200 5_3:-51896102 Msa0817030:five_prime_UTR
AGGTGAAGAAATTCAGGTTG+AGG 0.510992 5_3:-51896046 Msa0817030:five_prime_UTR
ATCTTAAATGGGAAATTGCT+CGG 0.515748 5_3:+51896117 None:intergenic
ACACTTAAAAGGACTTATCA+AGG 0.527097 5_3:-51896066 Msa0817030:five_prime_UTR
ACCTTCCACAGTGATAATAT+GGG 0.528877 5_3:+51895172 None:intergenic
ATGAACAGATTCCTTATGAC+GGG 0.541918 5_3:-51895816 Msa0817030:CDS
AGCACTTATATCCTTAGTAA+GGG 0.546945 5_3:-51895046 Msa0817030:three_prime_UTR
TGGAAGGTTTCATTCTTAGG+AGG 0.547935 5_3:-51895157 Msa0817030:three_prime_UTR
TGTCGATAACAATCTCATCT+GGG 0.591480 5_3:+51895869 None:intergenic
TCAGTGTTTCTAAGAAGGAT+GGG 0.595788 5_3:-51895921 Msa0817030:CDS
CACAAGTACTTAGAAATAAG+GGG 0.598764 5_3:-51895339 Msa0817030:CDS
ACACAACTGAACTCCAAAGA+GGG 0.618264 5_3:+51895898 None:intergenic
TTAAAGCATGAGCATTGACA+TGG 0.620599 5_3:-51895221 Msa0817030:exon
GATGAACAGATTCCTTATGA+CGG 0.641036 5_3:-51895817 Msa0817030:CDS
ATAGTAGAGAATACTTGGTG+TGG 0.641539 5_3:-51895266 Msa0817030:CDS
AACACAACTGAACTCCAAAG+AGG 0.648826 5_3:+51895897 None:intergenic
ATTGTATTATTGTGTCATTG+TGG 0.652680 5_3:-51895121 Msa0817030:three_prime_UTR
TTTCTCCCATATTATCACTG+TGG 0.682590 5_3:-51895177 Msa0817030:three_prime_UTR
GCATGAGTACATGATCAACA+AGG 0.729521 5_3:-51895289 Msa0817030:CDS
ATGGTCTGAAGTAATCACAG+AGG 0.774989 5_3:-51895202 Msa0817030:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGATTTTGGTTGATAT+AGG - chr5_3:51895019-51895038 Msa0817030:three_prime_UTR 20.0%
!!! AAATGATTTTGGTTGATATA+GGG - chr5_3:51895020-51895039 Msa0817030:three_prime_UTR 20.0%
!! GATTATCAACTATCTTAAAT+GGG + chr5_3:51895067-51895086 None:intergenic 20.0%
!!! TAATTGAAAGTATACAAACA+GGG + chr5_3:51895740-51895759 None:intergenic 20.0%
!!! TTAATTGAAAGTATACAAAC+AGG + chr5_3:51895741-51895760 None:intergenic 20.0%
!!! AAAATGATTTTGGTTGATAT+AGG - chr5_3:51895019-51895038 Msa0817030:three_prime_UTR 20.0%
!!! AAATGATTTTGGTTGATATA+GGG - chr5_3:51895020-51895039 Msa0817030:three_prime_UTR 20.0%
!! GATTATCAACTATCTTAAAT+GGG + chr5_3:51895067-51895086 None:intergenic 20.0%
!!! TAATTGAAAGTATACAAACA+GGG + chr5_3:51895740-51895759 None:intergenic 20.0%
!!! TTAATTGAAAGTATACAAAC+AGG + chr5_3:51895741-51895760 None:intergenic 20.0%
! GGATTATCAACTATCTTAAA+TGG + chr5_3:51895068-51895087 None:intergenic 25.0%
!!! TTTAAGATAGTTGATAATCC+AGG - chr5_3:51895068-51895087 Msa0817030:three_prime_UTR 25.0%
! ATCAGACAATAACACTTAAA+AGG - chr5_3:51895093-51895112 Msa0817030:three_prime_UTR 25.0%
!! ACATCAGTGTTGTTAAATAA+AGG - chr5_3:51895457-51895476 Msa0817030:intron 25.0%
!! TCAATATTTTTCATGTCTCT+AGG + chr5_3:51895677-51895696 None:intergenic 25.0%
!! ATTGTATTATTGTGTCATTG+TGG - chr5_3:51896049-51896068 Msa0817030:five_prime_UTR 25.0%
! ATGTAATAAATCCCTTACTA+AGG + chr5_3:51896138-51896157 None:intergenic 25.0%
! GGATTATCAACTATCTTAAA+TGG + chr5_3:51895068-51895087 None:intergenic 25.0%
!!! TTTAAGATAGTTGATAATCC+AGG - chr5_3:51895068-51895087 Msa0817030:three_prime_UTR 25.0%
! ATCAGACAATAACACTTAAA+AGG - chr5_3:51895093-51895112 Msa0817030:three_prime_UTR 25.0%
!! ACATCAGTGTTGTTAAATAA+AGG - chr5_3:51895457-51895476 Msa0817030:intron 25.0%
!! TCAATATTTTTCATGTCTCT+AGG + chr5_3:51895677-51895696 None:intergenic 25.0%
!! ATTGTATTATTGTGTCATTG+TGG - chr5_3:51896049-51896068 Msa0817030:five_prime_UTR 25.0%
! ATGTAATAAATCCCTTACTA+AGG + chr5_3:51896138-51896157 None:intergenic 25.0%
ATCTTAAATGGGAAATTGCT+CGG + chr5_3:51895056-51895075 None:intergenic 30.0%
ACACTTAAAAGGACTTATCA+AGG - chr5_3:51895104-51895123 Msa0817030:three_prime_UTR 30.0%
TTATCAAGGTGAAGAAATTC+AGG - chr5_3:51895118-51895137 Msa0817030:three_prime_UTR 30.0%
TGATCATCAAGATGATGATA+AGG - chr5_3:51895181-51895200 Msa0817030:three_prime_UTR 30.0%
ATCTGTTCATCAGAAAAATC+AGG + chr5_3:51895344-51895363 None:intergenic 30.0%
! AGAAGTACATACTGAAAATC+TGG + chr5_3:51895380-51895399 None:intergenic 30.0%
!!! TGCATTCAATGTTTTAATCC+AGG - chr5_3:51895431-51895450 Msa0817030:intron 30.0%
!!! GCATTCAATGTTTTAATCCA+GGG - chr5_3:51895432-51895451 Msa0817030:intron 30.0%
TTGTTAAATAAAGGCTACAG+CGG - chr5_3:51895466-51895485 Msa0817030:intron 30.0%
!!! GTTTTAAAAAGCAGCTATAG+TGG - chr5_3:51895555-51895574 Msa0817030:intron 30.0%
TCCACAAGTACTTAGAAATA+AGG - chr5_3:51895829-51895848 Msa0817030:CDS 30.0%
CCACAAGTACTTAGAAATAA+GGG - chr5_3:51895830-51895849 Msa0817030:CDS 30.0%
CACAAGTACTTAGAAATAAG+GGG - chr5_3:51895831-51895850 Msa0817030:CDS 30.0%
!! GTTTCTACTTCCTGTTTTAA+AGG - chr5_3:51896082-51896101 Msa0817030:five_prime_UTR 30.0%
TAGCACTTATATCCTTAGTA+AGG - chr5_3:51896123-51896142 Msa0817030:five_prime_UTR 30.0%
AGCACTTATATCCTTAGTAA+GGG - chr5_3:51896124-51896143 Msa0817030:five_prime_UTR 30.0%
ATCTTAAATGGGAAATTGCT+CGG + chr5_3:51895056-51895075 None:intergenic 30.0%
ACACTTAAAAGGACTTATCA+AGG - chr5_3:51895104-51895123 Msa0817030:three_prime_UTR 30.0%
TTATCAAGGTGAAGAAATTC+AGG - chr5_3:51895118-51895137 Msa0817030:three_prime_UTR 30.0%
TGATCATCAAGATGATGATA+AGG - chr5_3:51895181-51895200 Msa0817030:three_prime_UTR 30.0%
ATCTGTTCATCAGAAAAATC+AGG + chr5_3:51895344-51895363 None:intergenic 30.0%
! AGAAGTACATACTGAAAATC+TGG + chr5_3:51895380-51895399 None:intergenic 30.0%
!!! TGCATTCAATGTTTTAATCC+AGG - chr5_3:51895431-51895450 Msa0817030:intron 30.0%
!!! GCATTCAATGTTTTAATCCA+GGG - chr5_3:51895432-51895451 Msa0817030:intron 30.0%
TTGTTAAATAAAGGCTACAG+CGG - chr5_3:51895466-51895485 Msa0817030:intron 30.0%
!!! GTTTTAAAAAGCAGCTATAG+TGG - chr5_3:51895555-51895574 Msa0817030:intron 30.0%
TCCACAAGTACTTAGAAATA+AGG - chr5_3:51895829-51895848 Msa0817030:CDS 30.0%
CCACAAGTACTTAGAAATAA+GGG - chr5_3:51895830-51895849 Msa0817030:CDS 30.0%
CACAAGTACTTAGAAATAAG+GGG - chr5_3:51895831-51895850 Msa0817030:CDS 30.0%
!! GTTTCTACTTCCTGTTTTAA+AGG - chr5_3:51896082-51896101 Msa0817030:five_prime_UTR 30.0%
TAGCACTTATATCCTTAGTA+AGG - chr5_3:51896123-51896142 Msa0817030:five_prime_UTR 30.0%
AGCACTTATATCCTTAGTAA+GGG - chr5_3:51896124-51896143 Msa0817030:five_prime_UTR 30.0%
!!! ATTTTGGTTGATATAGGGTG+AGG - chr5_3:51895025-51895044 Msa0817030:three_prime_UTR 35.0%
! AGTGTTATTGTCTGATTTCC+TGG + chr5_3:51895089-51895108 None:intergenic 35.0%
! TTAGAAACACTGACTGAAAG+TGG + chr5_3:51895242-51895261 None:intergenic 35.0%
! TTCAGTCAGTGTTTCTAAGA+AGG - chr5_3:51895244-51895263 Msa0817030:CDS 35.0%
! TCAGTGTTTCTAAGAAGGAT+GGG - chr5_3:51895249-51895268 Msa0817030:CDS 35.0%
TGTCGATAACAATCTCATCT+GGG + chr5_3:51895304-51895323 None:intergenic 35.0%
GATGAACAGATTCCTTATGA+CGG - chr5_3:51895353-51895372 Msa0817030:CDS 35.0%
ATGAACAGATTCCTTATGAC+GGG - chr5_3:51895354-51895373 Msa0817030:CDS 35.0%
CCAATCTAAATTTCAGATCG+CGG + chr5_3:51895626-51895645 None:intergenic 35.0%
! CGCGATCTGAAATTTAGATT+GGG - chr5_3:51895624-51895643 Msa0817030:intron 35.0%
GCAGAATCTCATTCAAACAA+TGG + chr5_3:51895769-51895788 None:intergenic 35.0%
!!! TGAGATTCTGCCATTTTTAC+AGG - chr5_3:51895778-51895797 Msa0817030:intron 35.0%
!!! GAGATTCTGCCATTTTTACA+GGG - chr5_3:51895779-51895798 Msa0817030:intron 35.0%
CATCCAAATCCCTGTAAAAA+TGG + chr5_3:51895791-51895810 None:intergenic 35.0%
! TTTTTACAGGGATTTGGATG+AGG - chr5_3:51895791-51895810 Msa0817030:intron 35.0%
!! CCCTTATTTCTAAGTACTTG+TGG + chr5_3:51895833-51895852 None:intergenic 35.0%
!! TGCAAGAAATTTGTTGTGCT+TGG + chr5_3:51895864-51895883 None:intergenic 35.0%
CAAGGATAGTAGAGAATACT+TGG - chr5_3:51895899-51895918 Msa0817030:CDS 35.0%
! ATAGTAGAGAATACTTGGTG+TGG - chr5_3:51895904-51895923 Msa0817030:CDS 35.0%
!! TTAAAGCATGAGCATTGACA+TGG - chr5_3:51895949-51895968 Msa0817030:CDS 35.0%
TTTCTCCCATATTATCACTG+TGG - chr5_3:51895993-51896012 Msa0817030:CDS 35.0%
ACCTTCCACAGTGATAATAT+GGG + chr5_3:51896001-51896020 None:intergenic 35.0%
AACCTTCCACAGTGATAATA+TGG + chr5_3:51896002-51896021 None:intergenic 35.0%
! ATTGTGTCATTGTGGTTTAG+TGG - chr5_3:51896057-51896076 Msa0817030:five_prime_UTR 35.0%
!!! ATTTTGGTTGATATAGGGTG+AGG - chr5_3:51895025-51895044 Msa0817030:three_prime_UTR 35.0%
! AGTGTTATTGTCTGATTTCC+TGG + chr5_3:51895089-51895108 None:intergenic 35.0%
! TTAGAAACACTGACTGAAAG+TGG + chr5_3:51895242-51895261 None:intergenic 35.0%
! TTCAGTCAGTGTTTCTAAGA+AGG - chr5_3:51895244-51895263 Msa0817030:CDS 35.0%
! TCAGTGTTTCTAAGAAGGAT+GGG - chr5_3:51895249-51895268 Msa0817030:CDS 35.0%
TGTCGATAACAATCTCATCT+GGG + chr5_3:51895304-51895323 None:intergenic 35.0%
GATGAACAGATTCCTTATGA+CGG - chr5_3:51895353-51895372 Msa0817030:CDS 35.0%
ATGAACAGATTCCTTATGAC+GGG - chr5_3:51895354-51895373 Msa0817030:CDS 35.0%
CCAATCTAAATTTCAGATCG+CGG + chr5_3:51895626-51895645 None:intergenic 35.0%
! CGCGATCTGAAATTTAGATT+GGG - chr5_3:51895624-51895643 Msa0817030:intron 35.0%
GCAGAATCTCATTCAAACAA+TGG + chr5_3:51895769-51895788 None:intergenic 35.0%
!!! TGAGATTCTGCCATTTTTAC+AGG - chr5_3:51895778-51895797 Msa0817030:intron 35.0%
!!! GAGATTCTGCCATTTTTACA+GGG - chr5_3:51895779-51895798 Msa0817030:intron 35.0%
CATCCAAATCCCTGTAAAAA+TGG + chr5_3:51895791-51895810 None:intergenic 35.0%
! TTTTTACAGGGATTTGGATG+AGG - chr5_3:51895791-51895810 Msa0817030:intron 35.0%
!! CCCTTATTTCTAAGTACTTG+TGG + chr5_3:51895833-51895852 None:intergenic 35.0%
!! TGCAAGAAATTTGTTGTGCT+TGG + chr5_3:51895864-51895883 None:intergenic 35.0%
CAAGGATAGTAGAGAATACT+TGG - chr5_3:51895899-51895918 Msa0817030:CDS 35.0%
! ATAGTAGAGAATACTTGGTG+TGG - chr5_3:51895904-51895923 Msa0817030:CDS 35.0%
!! TTAAAGCATGAGCATTGACA+TGG - chr5_3:51895949-51895968 Msa0817030:CDS 35.0%
TTTCTCCCATATTATCACTG+TGG - chr5_3:51895993-51896012 Msa0817030:CDS 35.0%
ACCTTCCACAGTGATAATAT+GGG + chr5_3:51896001-51896020 None:intergenic 35.0%
AACCTTCCACAGTGATAATA+TGG + chr5_3:51896002-51896021 None:intergenic 35.0%
! ATTGTGTCATTGTGGTTTAG+TGG - chr5_3:51896057-51896076 Msa0817030:five_prime_UTR 35.0%
!! AGGTGAAGAAATTCAGGTTG+AGG - chr5_3:51895124-51895143 Msa0817030:three_prime_UTR 40.0%
TCTTCACCAGTAACCAAATC+AGG + chr5_3:51895158-51895177 None:intergenic 40.0%
! GTCAGTGTTTCTAAGAAGGA+TGG - chr5_3:51895248-51895267 Msa0817030:CDS 40.0%
ACACAACTGAACTCCAAAGA+GGG + chr5_3:51895275-51895294 None:intergenic 40.0%
AACACAACTGAACTCCAAAG+AGG + chr5_3:51895276-51895295 None:intergenic 40.0%
CTGTCGATAACAATCTCATC+TGG + chr5_3:51895305-51895324 None:intergenic 40.0%
TAACAACACTGATGTAACCC+TGG + chr5_3:51895452-51895471 None:intergenic 40.0%
TGCTATAGCACTTTGTGTAG+CGG - chr5_3:51895578-51895597 Msa0817030:intron 40.0%
! CCGCGATCTGAAATTTAGAT+TGG - chr5_3:51895623-51895642 Msa0817030:intron 40.0%
TGATGTTGCAGTTCAGATTG+TGG - chr5_3:51895694-51895713 Msa0817030:intron 40.0%
! TGCAGTTCAGATTGTGGTTT+TGG - chr5_3:51895700-51895719 Msa0817030:intron 40.0%
!!! CTGCCATTTTTACAGGGATT+TGG - chr5_3:51895785-51895804 Msa0817030:intron 40.0%
GCATGAGTACATGATCAACA+AGG - chr5_3:51895881-51895900 Msa0817030:CDS 40.0%
! ATGGTCTGAAGTAATCACAG+AGG - chr5_3:51895968-51895987 Msa0817030:CDS 40.0%
TCCCATATTATCACTGTGGA+AGG - chr5_3:51895997-51896016 Msa0817030:CDS 40.0%
CTGTGGAAGGTTTCATTCTT+AGG - chr5_3:51896010-51896029 Msa0817030:CDS 40.0%
TGGAAGGTTTCATTCTTAGG+AGG - chr5_3:51896013-51896032 Msa0817030:CDS 40.0%
!!! TTCCTGTTTTAAAGGCACAC+TGG - chr5_3:51896090-51896109 Msa0817030:five_prime_UTR 40.0%
!! AACCAGTGTGCCTTTAAAAC+AGG + chr5_3:51896095-51896114 None:intergenic 40.0%
!! AGGTGAAGAAATTCAGGTTG+AGG - chr5_3:51895124-51895143 Msa0817030:three_prime_UTR 40.0%
TCTTCACCAGTAACCAAATC+AGG + chr5_3:51895158-51895177 None:intergenic 40.0%
! GTCAGTGTTTCTAAGAAGGA+TGG - chr5_3:51895248-51895267 Msa0817030:CDS 40.0%
ACACAACTGAACTCCAAAGA+GGG + chr5_3:51895275-51895294 None:intergenic 40.0%
AACACAACTGAACTCCAAAG+AGG + chr5_3:51895276-51895295 None:intergenic 40.0%
CTGTCGATAACAATCTCATC+TGG + chr5_3:51895305-51895324 None:intergenic 40.0%
TAACAACACTGATGTAACCC+TGG + chr5_3:51895452-51895471 None:intergenic 40.0%
TGCTATAGCACTTTGTGTAG+CGG - chr5_3:51895578-51895597 Msa0817030:intron 40.0%
! CCGCGATCTGAAATTTAGAT+TGG - chr5_3:51895623-51895642 Msa0817030:intron 40.0%
TGATGTTGCAGTTCAGATTG+TGG - chr5_3:51895694-51895713 Msa0817030:intron 40.0%
! TGCAGTTCAGATTGTGGTTT+TGG - chr5_3:51895700-51895719 Msa0817030:intron 40.0%
!!! CTGCCATTTTTACAGGGATT+TGG - chr5_3:51895785-51895804 Msa0817030:intron 40.0%
GCATGAGTACATGATCAACA+AGG - chr5_3:51895881-51895900 Msa0817030:CDS 40.0%
! ATGGTCTGAAGTAATCACAG+AGG - chr5_3:51895968-51895987 Msa0817030:CDS 40.0%
TCCCATATTATCACTGTGGA+AGG - chr5_3:51895997-51896016 Msa0817030:CDS 40.0%
CTGTGGAAGGTTTCATTCTT+AGG - chr5_3:51896010-51896029 Msa0817030:CDS 40.0%
TGGAAGGTTTCATTCTTAGG+AGG - chr5_3:51896013-51896032 Msa0817030:CDS 40.0%
!!! TTCCTGTTTTAAAGGCACAC+TGG - chr5_3:51896090-51896109 Msa0817030:five_prime_UTR 40.0%
!! AACCAGTGTGCCTTTAAAAC+AGG + chr5_3:51896095-51896114 None:intergenic 40.0%
TGAGGTTCACATGCCTGATT+TGG - chr5_3:51895142-51895161 Msa0817030:three_prime_UTR 45.0%
!! CACATGCCTGATTTGGTTAC+TGG - chr5_3:51895149-51895168 Msa0817030:three_prime_UTR 45.0%
TGAAAATCTGGCCCGTCATA+AGG + chr5_3:51895368-51895387 None:intergenic 45.0%
TGTTGCTAACCTGTACTGCT+TGG - chr5_3:51895650-51895669 Msa0817030:intron 45.0%
TGAGGTTCACATGCCTGATT+TGG - chr5_3:51895142-51895161 Msa0817030:three_prime_UTR 45.0%
!! CACATGCCTGATTTGGTTAC+TGG - chr5_3:51895149-51895168 Msa0817030:three_prime_UTR 45.0%
TGAAAATCTGGCCCGTCATA+AGG + chr5_3:51895368-51895387 None:intergenic 45.0%
TGTTGCTAACCTGTACTGCT+TGG - chr5_3:51895650-51895669 Msa0817030:intron 45.0%
TAAGAAGGATGGGCCCTCTT+TGG - chr5_3:51895259-51895278 Msa0817030:CDS 50.0%
!! TCTCTAGGACCAAGCAGTAC+AGG + chr5_3:51895662-51895681 None:intergenic 50.0%
TAAGAAGGATGGGCCCTCTT+TGG - chr5_3:51895259-51895278 Msa0817030:CDS 50.0%
!! TCTCTAGGACCAAGCAGTAC+AGG + chr5_3:51895662-51895681 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 51895016 51896176 51895016 ID=Msa0817030;Name=Msa0817030
chr5_3 mRNA 51895016 51896176 51895016 ID=Msa0817030-mRNA-1;Parent=Msa0817030;Name=Msa0817030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=136|1|1|1|1|1|2|217|131
chr5_3 exon 51895016 51895392 51895016 ID=Msa0817030-mRNA-1:exon:15610;Parent=Msa0817030-mRNA-1
chr5_3 exon 51895805 51896176 51895805 ID=Msa0817030-mRNA-1:exon:15609;Parent=Msa0817030-mRNA-1
chr5_3 five_prime_UTR 51896041 51896176 51896041 ID=Msa0817030-mRNA-1:five_prime_utr;Parent=Msa0817030-mRNA-1
chr5_3 CDS 51895805 51896040 51895805 ID=Msa0817030-mRNA-1:cds;Parent=Msa0817030-mRNA-1
chr5_3 CDS 51895233 51895392 51895233 ID=Msa0817030-mRNA-1:cds;Parent=Msa0817030-mRNA-1
chr5_3 three_prime_UTR 51895016 51895232 51895016 ID=Msa0817030-mRNA-1:three_prime_utr;Parent=Msa0817030-mRNA-1
Gene Sequence

>Msa0817030

ATGCCTGATTTGGTTACTGGTGAAGAACATGATCATCAAGATGATGATAAGGATGATGAGAGTGAGAGAGCAACTCAATCAAGCATTCCACTTTCAGTCAGTGTTTCTAAGAAGGATGGGCCCTCTTTGGAGTTCAGTTGTGTTGCTTACCCAGATGAGATTGTTATCGACAGCTTGTCTGTAAAGAATCCTGATTTTTCTGATGAACAGATTCCTTATGACGGGCCAGATTTTCAGGATTTGGATGAGGCATTGCAAAAGTCATTCCACAAGTACTTAGAAATAAGGGGAATCAAACCAAGCACAACAAATTTCTTGCATGAGTACATGATCAACAAGGATAGTAGAGAATACTTGGTGTGGTTGAATAAGCTCAAGAATTTCATTAAAGCATGA
Protein sequence

>Msa0817030

MPDLVTGEEHDHQDDDKDDESERATQSSIPLSVSVSKKDGPSLEFSCVAYPDEIVIDSLSVKNPDFSDEQIPYDGPDFQDLDEALQKSFHKYLEIRGIKPSTTNFLHEYMINKDSREYLVWLNKLKNFIKA*