AlfalfaGEDB | Msa0818910

M. sativa cultivar zhongmu-4 / Msa0818910

KEGG: K15227
GO: -
PFAM: PF02153.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0818910	XP_003616719.1	70.513	78	18	2	21	93	85	162	4.40e-23	108
Msa0818910	XP_003616719.1	95.113	266	13	0	96	361	1	266	0.0	527

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0818910	sp\|Q944B6\|TYRA1_ARATH	56.439	264	110	2	96	355	359	621	4.46e-98	306
Msa0818910	sp\|Q944B6\|TYRA1_ARATH	50.000	260	123	3	99	352	50	308	1.94e-81	263
Msa0818910	sp\|Q944B6\|TYRA1_ARATH	49.367	79	32	3	23	93	445	523	2.55e-11	68.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0818910	G7K796	70.513	78	18	2	21	93	85	162	2.10e-23	108
Msa0818910	G7K796	95.113	266	13	0	96	361	1	266	0.0	527

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0054730	Msa0818910	-0.813775	2.157992e-51	-8.615850e-47
Msa0106370	Msa0818910	-0.816545	5.233082e-52	-8.615850e-47
Msa0818910	Msa0836680	0.807509	4.882217e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0818910	MtrunA17_Chr5g0437781	95.113	266	13	0	96	361	1	266	0.0	527
Msa0818910	MtrunA17_Chr5g0437781	70.513	78	18	2	21	93	85	162	4.04e-27	108
Msa0818910	MtrunA17_Chr3g0114251	76.515	264	61	1	97	360	3	265	2.71e-148	419
Msa0818910	MtrunA17_Chr3g0114251	64.935	77	23	2	21	93	86	162	2.75e-23	97.8
Msa0818910	MtrunA17_Chr4g0064761	54.440	259	113	3	98	352	379	636	1.24e-88	280
Msa0818910	MtrunA17_Chr4g0064761	47.535	284	142	3	75	354	48	328	1.36e-86	275
Msa0818910	MtrunA17_Chr5g0437791	92.135	89	7	0	5	93	14	102	4.00e-55	176
Msa0818910	MtrunA17_Chr5g0437791	60.714	84	28	2	174	257	24	102	3.40e-24	95.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0818910	AT5G34930.3	56.439	264	110	2	96	355	339	601	2.23e-99	307
Msa0818910	AT5G34930.3	50.000	260	123	3	99	352	30	288	1.03e-82	264
Msa0818910	AT5G34930.3	49.367	79	32	3	23	93	425	503	2.41e-12	68.6
Msa0818910	AT5G34930.1	56.439	264	110	2	96	355	359	621	4.53e-99	306
Msa0818910	AT5G34930.1	50.000	260	123	3	99	352	50	308	1.97e-82	263
Msa0818910	AT5G34930.1	49.367	79	32	3	23	93	445	523	2.59e-12	68.6
Msa0818910	AT5G34930.2	56.439	264	110	2	96	355	381	643	9.45e-99	306
Msa0818910	AT5G34930.2	50.000	260	123	3	99	352	72	330	3.50e-82	263
Msa0818910	AT5G34930.2	49.367	79	32	3	23	93	467	545	2.86e-12	68.6
Msa0818910	AT1G15710.1	49.301	286	138	3	74	355	33	315	2.35e-93	283

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Find 80 sgRNAs with CRISPR-Local

Find 546 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACATCAAGTGGTGT+TGG	0.560732	5_3:+55015754	None:intergenic
AAAATTGGCATAGTTGGATT+TGG	0.317252	5_3:-55013619	Msa0818910:CDS
AATATGCAGAAAATTAGAGC+AGG	0.590082	5_3:+55012736	None:intergenic
AATCCATAATGATCAAGTAA+TGG	0.560778	5_3:+55011598	None:intergenic
AATTCACCTTCTCTGAAAAT+TGG	0.228956	5_3:-55013634	Msa0818910:CDS
AATTGGGCATTCAATTCTTC+AGG	0.395208	5_3:-55013504	Msa0818910:intron
AATTGTGAATGTGTCAGCTA+TGG	0.625758	5_3:-55011564	Msa0818910:three_prime_UTR
ACATCAACAAAGAGTGTTGT+TGG	0.383652	5_3:+55013284	None:intergenic
ACATGGTCAACAAGATTATT+AGG	0.422140	5_3:+55011690	None:intergenic
ACCCGCAATAAAAGTTCTCT+TGG	0.385892	5_3:+55013242	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGACTAAAAGAACATCAAG+TGG	+	chr5_3:55011495-55011514	None:intergenic	30.0%
CCTCATTAGCAAAAAAATTG+TGG	+	chr5_3:55011671-55011690	None:intergenic	30.0%
GAGTTAATGACATATGACTA+AGG	+	chr5_3:55011704-55011723	None:intergenic	30.0%
ATGTAACAATGTCGATGAAT+CGG	+	chr5_3:55011859-55011878	None:intergenic	30.0%
AGTTTGGAAAACTTGAAACA+AGG	+	chr5_3:55012067-55012086	None:intergenic	30.0%
CTTCATCCTAAAATAGGATT+AGG	+	chr5_3:55012198-55012217	None:intergenic	30.0%
AAAGTGATGATCACGATTAA+GGG	+	chr5_3:55012604-55012623	None:intergenic	30.0%
AAAAGTGATGATCACGATTA+AGG	+	chr5_3:55012605-55012624	None:intergenic	30.0%
GGTTCAAAATCATTGTTCTT+TGG	-	chr5_3:55012974-55012993	Msa0818910:intron	30.0%
TTTACCAAAGGACCTTAAAT+AGG	-	chr5_3:55013053-55013072	Msa0818910:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	55011411	55015851	55011411	ID=Msa0818910;Name=Msa0818910
chr5_3	mRNA	55011411	55015851	55011411	ID=Msa0818910-mRNA-1;Parent=Msa0818910;Name=Msa0818910-mRNA-1;_AED=0.20;_eAED=0.21;_QI=0\|0.85\|0.75\|1\|1\|1\|8\|311\|361
chr5_3	exon	55011411	55011808	55011411	ID=Msa0818910-mRNA-1:exon:16686;Parent=Msa0818910-mRNA-1
chr5_3	exon	55011956	55012030	55011956	ID=Msa0818910-mRNA-1:exon:16685;Parent=Msa0818910-mRNA-1
chr5_3	exon	55012115	55012184	55012115	ID=Msa0818910-mRNA-1:exon:16684;Parent=Msa0818910-mRNA-1
chr5_3	exon	55012269	55012351	55012269	ID=Msa0818910-mRNA-1:exon:16683;Parent=Msa0818910-mRNA-1
chr5_3	exon	55012724	55012879	55012724	ID=Msa0818910-mRNA-1:exon:16682;Parent=Msa0818910-mRNA-1
chr5_3	exon	55013244	55013415	55013244	ID=Msa0818910-mRNA-1:exon:16681;Parent=Msa0818910-mRNA-1
chr5_3	exon	55013505	55013668	55013505	ID=Msa0818910-mRNA-1:exon:16680;Parent=Msa0818910-mRNA-1
chr5_3	exon	55015573	55015851	55015573	ID=Msa0818910-mRNA-1:exon:16679;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55015573	55015851	55015573	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55013505	55013668	55013505	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55013244	55013415	55013244	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55012724	55012879	55012724	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55012269	55012351	55012269	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55012115	55012184	55012115	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55011956	55012030	55011956	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	CDS	55011722	55011808	55011722	ID=Msa0818910-mRNA-1:cds;Parent=Msa0818910-mRNA-1
chr5_3	three_prime_UTR	55011411	55011721	55011411	ID=Msa0818910-mRNA-1:three_prime_utr;Parent=Msa0818910-mRNA-1

Gene Sequence

>Msa0818910

ATGTCATGCTCATATGCACATTCATTCTCTCTTTTTCCGAGGTTCTCAGTTCCGTCATGTGCTTGTCTTAAGCGACCAACACCACTTGATGTTCTTTTAGTCAAAAAACATCCAAGAGACCTCTTACTACCAGAAGAGTCAGGTATACTCTGCACACACCTAATGGTTGGACCAGAGAGTGGAAAGGATGGGTGCAAAGATCTCACTTTCATGTATGATAAAGTTAGAATATGTGATGAAGCTAACTGCTCTAATTTCCACAATTTTTTTGCTAATGAGCCAATCATGTCAAATTCACCTTCTCTGAAAATTGGCATAGTTGGATTTGGTTCCTTTGGTCAATTTCTGGCAAAGACTATGATCAAACAAGGCCACACTCTAACTGCAACTTCAAGAGCTGATTACTCTCACACATGTCTCCAATTGGGCATTCAATTCTTCAGGGATGTTAGAACATTCATTGAGGCCAACAACGATGTCATACTCATATGCACATCCATTCTGTCTTTTTCTAAGGTTCTCAGTTCTATGTCATTTGGTTGTCTTAAGAAACCAACAACACTCTTTGTTGATGTTCTTTCAGTCAAAGAACACCCAAGAGAACTTTTATTGCGGGTTCTGCCAGAGGAGTCAGATATACTCTGCACACACCCAATGTTTGGACCAGAAAGTGGAAAGAATGGGTGGAATAATCTCAATTTCATGTATGATAAAGTTAGAATGCGTGATGAAGCTACCTGCTCTAATTTTCTGCATATTTTTGCTAGTGAGGGTTGTAAGATGCTACAAATGTCCTGTGAAGAACATGATAAAATAGCTGCAAAGAGCCAATTTATAACACACACTATAGGCAGGACATTGGCAGAAATGGATATTGAGTCCACACCTATTGATACAAAAGGCTTTCAAACACTTACTCAACTGAAGAACACCACCATGAGAGATAGTTTTGATCTGTACAGTGGATTATTTGTACATAACAGATTCGCTAAGCAAGAGCTGGAAAACCTCCAACGTGCCCTAGACAAAGTTAAAGAGATGCTGGTTCAAAGGATGAGAGAGGAGCTGGGTCCAGAAAAAGACTGA

Protein sequence

>Msa0818910

MSCSYAHSFSLFPRFSVPSCACLKRPTPLDVLLVKKHPRDLLLPEESGILCTHLMVGPESGKDGCKDLTFMYDKVRICDEANCSNFHNFFANEPIMSNSPSLKIGIVGFGSFGQFLAKTMIKQGHTLTATSRADYSHTCLQLGIQFFRDVRTFIEANNDVILICTSILSFSKVLSSMSFGCLKKPTTLFVDVLSVKEHPRELLLRVLPEESDILCTHPMFGPESGKNGWNNLNFMYDKVRMRDEATCSNFLHIFASEGCKMLQMSCEEHDKIAAKSQFITHTIGRTLAEMDIESTPIDTKGFQTLTQLKNTTMRDSFDLYSGLFVHNRFAKQELENLQRALDKVKEMLVQRMREELGPEKD*