Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820150 | XP_039685710.1 | 98.667 | 75 | 1 | 0 | 1 | 75 | 154 | 228 | 3.01e-42 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820150 | sp|Q39238|TSL_ARATH | 74.667 | 75 | 19 | 0 | 1 | 75 | 236 | 310 | 9.98e-30 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820150 | A0A396HY40 | 71.552 | 116 | 2 | 1 | 1 | 85 | 108 | 223 | 1.98e-41 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0694370 | Msa0820150 | 0.807758 | 4.323118e-50 | -8.615850e-47 |
| Msa0743810 | Msa0820150 | 0.804233 | 2.383424e-49 | -8.615850e-47 |
| Msa0820150 | Msa0885290 | 0.804137 | 2.495656e-49 | -8.615850e-47 |
| Msa0820150 | Msa0935910 | 0.800833 | 1.196966e-48 | -8.615850e-47 |
| Msa0820150 | Msa1287800 | 0.805355 | 1.389182e-49 | -8.615850e-47 |
| Msa0820150 | Msa1320300 | 0.805545 | 1.267470e-49 | -8.615850e-47 |
| Msa0188460 | Msa0820150 | 0.810350 | 1.204003e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820150 | MtrunA17_Chr5g0440131 | 71.552 | 116 | 2 | 1 | 1 | 85 | 108 | 223 | 3.81e-45 | 152 |
| Msa0820150 | MtrunA17_Chr5g0440001 | 98.667 | 75 | 1 | 0 | 1 | 75 | 220 | 294 | 6.80e-43 | 147 |
| Msa0820150 | MtrunA17_Chr0c14g0493691 | 88.158 | 76 | 8 | 1 | 12 | 86 | 1 | 76 | 8.13e-42 | 131 |
| Msa0820150 | MtrunA17_Chr5g0440171 | 94.521 | 73 | 4 | 0 | 3 | 75 | 155 | 227 | 9.24e-40 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820150 | AT5G20930.1 | 74.667 | 75 | 19 | 0 | 1 | 75 | 236 | 310 | 1.01e-30 | 113 |
Find 19 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAACCTTAGGTTTCATT+TGG | 0.341893 | 5_3:-57385479 | None:intergenic |
| GGAAGATGGTCAGGCGTTAA+AGG | 0.346557 | 5_3:+57385990 | Msa0820150:CDS |
| TTATTCTGCTCACTTTCTAA+TGG | 0.352740 | 5_3:+57386038 | Msa0820150:CDS |
| TCGGAAATGTTGGTGTAATC+AGG | 0.407160 | 5_3:+57385859 | Msa0820150:CDS |
| CAGAATAATGTGATACAAGC+TGG | 0.466892 | 5_3:-57386023 | None:intergenic |
| TGAGACCTGGGAAGATGGTC+AGG | 0.479421 | 5_3:+57385981 | Msa0820150:CDS |
| TGAAACTGATATCAGCAAAT+CGG | 0.484786 | 5_3:-57385784 | None:intergenic |
| TTATCTGAGACCTGGGAAGA+TGG | 0.512235 | 5_3:+57385976 | Msa0820150:CDS |
| AGCAAGATTAAAAGTACGAC+AGG | 0.515934 | 5_3:+57385824 | Msa0820150:CDS |
| TTTGCTGATATCAGTTTCAA+AGG | 0.526129 | 5_3:+57385788 | Msa0820150:CDS |
| GAAGGAATTGAAAGATCTTG+AGG | 0.541001 | 5_3:+57385455 | Msa0820150:CDS |
| TTGGCACATGTAGGAACTGA+AGG | 0.546431 | 5_3:+57385437 | None:intergenic |
| TGAGGACCAAATGAAACCTA+AGG | 0.546600 | 5_3:+57385473 | Msa0820150:CDS |
| AACTGTCTTATCTGAGACCT+GGG | 0.549763 | 5_3:+57385969 | Msa0820150:CDS |
| GAACTGTCTTATCTGAGACC+TGG | 0.560151 | 5_3:+57385968 | Msa0820150:CDS |
| TCATTGAGACTCGGAAATGT+TGG | 0.563510 | 5_3:+57385849 | Msa0820150:CDS |
| TAACGCCTGACCATCTTCCC+AGG | 0.569356 | 5_3:-57385986 | None:intergenic |
| AGAATAATGTGATACAAGCT+GGG | 0.596992 | 5_3:-57386022 | None:intergenic |
| CGACAGGACTCATTGAGACT+CGG | 0.604297 | 5_3:+57385840 | Msa0820150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATAAAAATATTATAAAA+AGG | - | chr5_3:57385910-57385929 | None:intergenic | 0.0% |
| !!! | TTTTTATAATATTTTTATTT+AGG | + | chr5_3:57385909-57385928 | Msa0820150:intron | 0.0% |
| !!! | TTATTTTTATCTTTTGATTG+TGG | + | chr5_3:57385501-57385520 | Msa0820150:intron | 15.0% |
| !!! | TCATTTAAAATATAATGAAG+TGG | + | chr5_3:57385578-57385597 | Msa0820150:intron | 15.0% |
| !!! | TTTTATTTAGGTTTTCTTAT+TGG | + | chr5_3:57385921-57385940 | Msa0820150:intron | 15.0% |
| !! | GATAAAAATAATCAAACCTT+AGG | - | chr5_3:57385492-57385511 | None:intergenic | 20.0% |
| !!! | TTTAAAATATAATGAAGTGG+TGG | + | chr5_3:57385581-57385600 | Msa0820150:intron | 20.0% |
| !!! | ATGAACTAGAAATTTTAAAG+AGG | + | chr5_3:57385642-57385661 | Msa0820150:intron | 20.0% |
| !!! | TATTATATGAATTTTAGAGC+TGG | + | chr5_3:57385946-57385965 | Msa0820150:intron | 20.0% |
| ! | TAAATGACATGTATACCAAT+AGG | - | chr5_3:57385565-57385584 | None:intergenic | 25.0% |
| ! | TCTTACTCCAAAATAAGTTA+AGG | + | chr5_3:57385674-57385693 | Msa0820150:intron | 25.0% |
| ! | GTATAAAAATTAAGCTAAGC+CGG | + | chr5_3:57385714-57385733 | Msa0820150:intron | 25.0% |
| ! | TTTGTTCTCTACAAAATTCA+AGG | - | chr5_3:57385756-57385775 | None:intergenic | 25.0% |
| ATCAAACCTTAGGTTTCATT+TGG | - | chr5_3:57385482-57385501 | None:intergenic | 30.0% | |
| ! | CAATGCTCCTTAACTTATTT+TGG | - | chr5_3:57385684-57385703 | None:intergenic | 30.0% |
| TGAAACTGATATCAGCAAAT+CGG | - | chr5_3:57385787-57385806 | None:intergenic | 30.0% | |
| TTTGCTGATATCAGTTTCAA+AGG | + | chr5_3:57385788-57385807 | Msa0820150:CDS | 30.0% | |
| AGAATAATGTGATACAAGCT+GGG | - | chr5_3:57386025-57386044 | None:intergenic | 30.0% | |
| TTATTCTGCTCACTTTCTAA+TGG | + | chr5_3:57386038-57386057 | Msa0820150:CDS | 30.0% | |
| GAAGGAATTGAAAGATCTTG+AGG | + | chr5_3:57385455-57385474 | Msa0820150:CDS | 35.0% | |
| ! | TTTTGTGTCTTGAGAGAATC+GGG | - | chr5_3:57385540-57385559 | None:intergenic | 35.0% |
| ! | GTTTTGTGTCTTGAGAGAAT+CGG | - | chr5_3:57385541-57385560 | None:intergenic | 35.0% |
| TCAAGACACAAAACTCCTAT+TGG | + | chr5_3:57385547-57385566 | Msa0820150:intron | 35.0% | |
| TCTCTACAAAATTCAAGGAC+TGG | - | chr5_3:57385751-57385770 | None:intergenic | 35.0% | |
| ! | AGCAAGATTAAAAGTACGAC+AGG | + | chr5_3:57385824-57385843 | Msa0820150:CDS | 35.0% |
| ATGTTGGTGTAATCAGGTAT+AGG | + | chr5_3:57385865-57385884 | Msa0820150:intron | 35.0% | |
| CAGAATAATGTGATACAAGC+TGG | - | chr5_3:57386026-57386045 | None:intergenic | 35.0% | |
| TGAGGACCAAATGAAACCTA+AGG | + | chr5_3:57385473-57385492 | Msa0820150:CDS | 40.0% | |
| AGGACTGGATGTTAATATGC+CGG | - | chr5_3:57385736-57385755 | None:intergenic | 40.0% | |
| TCATTGAGACTCGGAAATGT+TGG | + | chr5_3:57385849-57385868 | Msa0820150:CDS | 40.0% | |
| TCGGAAATGTTGGTGTAATC+AGG | + | chr5_3:57385859-57385878 | Msa0820150:CDS | 40.0% | |
| ! | AACTGTCTTATCTGAGACCT+GGG | + | chr5_3:57385969-57385988 | Msa0820150:CDS | 40.0% |
| ! | GAACTGTCTTATCTGAGACC+TGG | + | chr5_3:57385968-57385987 | Msa0820150:CDS | 45.0% |
| ! | TTATCTGAGACCTGGGAAGA+TGG | + | chr5_3:57385976-57385995 | Msa0820150:CDS | 45.0% |
| GTGTCTTGAGAGAATCGGGA+AGG | - | chr5_3:57385536-57385555 | None:intergenic | 50.0% | |
| CGACAGGACTCATTGAGACT+CGG | + | chr5_3:57385840-57385859 | Msa0820150:CDS | 50.0% | |
| GGAAGATGGTCAGGCGTTAA+AGG | + | chr5_3:57385990-57386009 | Msa0820150:CDS | 50.0% | |
| TGAGACCTGGGAAGATGGTC+AGG | + | chr5_3:57385981-57386000 | Msa0820150:CDS | 55.0% | |
| TAACGCCTGACCATCTTCCC+AGG | - | chr5_3:57385989-57386008 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 57385450 | 57386065 | 57385450 | ID=Msa0820150;Name=Msa0820150 |
| chr5_3 | mRNA | 57385450 | 57386065 | 57385450 | ID=Msa0820150-mRNA-1;Parent=Msa0820150;Name=Msa0820150-mRNA-1;_AED=0.41;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|86 |
| chr5_3 | exon | 57385450 | 57385494 | 57385450 | ID=Msa0820150-mRNA-1:exon:17320;Parent=Msa0820150-mRNA-1 |
| chr5_3 | exon | 57385768 | 57385880 | 57385768 | ID=Msa0820150-mRNA-1:exon:17321;Parent=Msa0820150-mRNA-1 |
| chr5_3 | exon | 57385963 | 57386065 | 57385963 | ID=Msa0820150-mRNA-1:exon:17322;Parent=Msa0820150-mRNA-1 |
| chr5_3 | CDS | 57385450 | 57385494 | 57385450 | ID=Msa0820150-mRNA-1:cds;Parent=Msa0820150-mRNA-1 |
| chr5_3 | CDS | 57385768 | 57385880 | 57385768 | ID=Msa0820150-mRNA-1:cds;Parent=Msa0820150-mRNA-1 |
| chr5_3 | CDS | 57385963 | 57386065 | 57385963 | ID=Msa0820150-mRNA-1:cds;Parent=Msa0820150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
