AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0821460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821460 MtrunA17_Chr5g0433561 100.000 200 0 0 1 200 1 200 2.05e-150 415
Msa0821460 MtrunA17_Chr3g0109721 93.500 200 13 0 1 200 1 200 3.05e-143 397
Msa0821460 MtrunA17_Chr8g0390561 67.358 193 62 1 8 200 7 198 7.67e-97 279
Msa0821460 MtrunA17_Chr1g0199331 65.269 167 57 1 8 173 29 195 1.20e-78 233
Msa0821460 MtrunA17_Chr4g0055651 42.268 194 105 3 12 200 10 201 2.36e-50 161
Msa0821460 MtrunA17_Chr3g0123971 44.099 161 90 0 12 172 11 171 1.67e-49 159
Msa0821460 MtrunA17_Chr4g0032201 42.781 187 99 2 12 193 14 197 2.40e-49 159
Msa0821460 MtrunA17_Chr8g0382221 44.099 161 90 0 12 172 11 171 2.89e-49 159
Msa0821460 MtrunA17_Chr6g0458081 44.375 160 89 0 12 171 8 167 3.59e-49 159
Msa0821460 MtrunA17_Chr3g0112681 41.584 202 105 3 12 200 14 215 5.88e-49 158
Msa0821460 MtrunA17_Chr8g0381841 40.976 205 104 4 12 199 19 223 8.79e-49 158
Msa0821460 MtrunA17_Chr4g0040781 38.614 202 111 2 12 200 15 216 9.55e-49 158
Msa0821460 MtrunA17_Chr4g0046261 39.109 202 110 2 12 200 15 216 4.86e-48 156
Msa0821460 MtrunA17_Chr2g0283131 38.614 202 111 2 12 200 15 216 6.80e-48 155
Msa0821460 MtrunA17_Chr2g0315551 39.806 206 103 3 12 200 15 216 9.83e-48 155
Msa0821460 MtrunA17_Chr3g0123821 39.819 221 111 5 1 199 1 221 1.02e-47 155
Msa0821460 MtrunA17_Chr2g0276941 48.366 153 79 0 12 164 17 169 2.55e-47 154
Msa0821460 MtrunA17_Chr6g0482721 39.604 202 109 2 12 200 14 215 3.25e-47 154
Msa0821460 MtrunA17_Chr2g0319951 44.444 162 90 0 12 173 15 176 7.36e-47 153
Msa0821460 MtrunA17_Chr8g0349711 41.875 160 93 0 12 171 8 167 8.95e-47 153
Msa0821460 MtrunA17_Chr5g0400131 39.773 176 106 0 12 187 11 186 1.37e-46 152
Msa0821460 MtrunA17_Chr8g0349681 37.688 199 114 1 12 200 386 584 1.61e-46 161
Msa0821460 MtrunA17_Chr8g0349681 41.358 162 95 0 12 173 177 338 5.09e-43 151
Msa0821460 MtrunA17_Chr8g0349681 41.830 153 89 0 12 164 15 167 4.33e-41 146
Msa0821460 MtrunA17_Chr2g0290891 42.500 160 92 0 12 171 8 167 2.65e-46 151
Msa0821460 MtrunA17_Chr7g0250531 39.583 192 113 1 12 200 10 201 3.59e-46 151
Msa0821460 MtrunA17_Chr4g0055081 41.615 161 94 0 12 172 11 171 4.50e-46 150
Msa0821460 MtrunA17_Chr7g0275991 41.718 163 95 0 12 174 22 184 4.81e-46 151
Msa0821460 MtrunA17_Chr8g0346421 41.899 179 99 1 12 185 28 206 8.58e-46 151
Msa0821460 MtrunA17_Chr1g0189361 37.981 208 110 3 12 200 16 223 1.65e-45 150
Msa0821460 MtrunA17_Chr8g0351861 46.405 153 82 0 12 164 18 170 2.77e-45 149
Msa0821460 MtrunA17_Chr1g0182301 37.688 199 112 4 12 199 17 214 6.44e-45 148
Msa0821460 MtrunA17_Chr7g0231131 37.931 203 111 2 12 199 17 219 8.23e-45 148
Msa0821460 MtrunA17_Chr8g0335471 40.000 195 109 3 12 200 10 202 1.08e-44 147
Msa0821460 MtrunA17_Chr1g0182321 37.688 199 112 4 12 199 17 214 1.40e-44 147
Msa0821460 MtrunA17_Chr1g0174281 40.782 179 98 1 12 182 17 195 2.79e-44 147
Msa0821460 MtrunA17_Chr7g0234201 38.049 205 107 4 12 200 15 215 7.97e-44 145
Msa0821460 MtrunA17_Chr2g0325091 42.500 160 92 0 12 171 13 172 1.08e-43 145
Msa0821460 MtrunA17_Chr2g0276931 40.936 171 100 1 12 182 11 180 1.53e-43 144
Msa0821460 MtrunA17_Chr1g0212521 38.384 198 112 4 12 199 17 214 1.83e-43 144
Msa0821460 MtrunA17_Chr4g0030911 37.056 197 115 3 12 199 17 213 9.54e-43 142
Msa0821460 MtrunA17_Chr7g0263071 41.104 163 95 1 12 173 17 179 2.73e-42 141
Msa0821460 MtrunA17_Chr7g0214241 40.000 160 96 0 12 171 17 176 1.56e-41 139
Msa0821460 MtrunA17_Chr8g0384311 38.974 195 111 5 12 199 15 208 2.28e-39 134
Msa0821460 MtrunA17_Chr8g0392931 45.513 156 85 0 12 167 16 171 8.08e-39 132
Msa0821460 MtrunA17_Chr2g0327911 33.673 196 122 4 12 199 17 212 8.30e-39 132
Msa0821460 MtrunA17_Chr7g0265001 37.624 202 116 4 8 200 14 214 9.65e-38 130
Msa0821460 MtrunA17_Chr8g0383261 36.082 194 117 4 12 198 14 207 9.64e-37 127
Msa0821460 MtrunA17_Chr1g0159481 37.563 197 114 6 12 200 19 214 1.02e-36 127
Msa0821460 MtrunA17_Chr1g0197331 35.885 209 123 6 2 200 5 212 1.64e-36 126
Msa0821460 MtrunA17_Chr1g0180291 37.313 201 115 5 8 199 12 210 1.92e-36 126
Msa0821460 MtrunA17_Chr3g0130271 37.245 196 114 6 12 199 14 208 2.48e-35 123
Msa0821460 MtrunA17_Chr6g0466301 39.394 165 93 3 12 169 10 174 4.26e-35 122
Msa0821460 MtrunA17_Chr6g0465491 39.394 165 93 3 12 169 10 174 4.26e-35 122
Msa0821460 MtrunA17_Chr1g0201121 39.394 165 93 3 12 169 10 174 9.79e-34 119
Msa0821460 MtrunA17_Chr4g0034871 38.182 165 95 3 12 169 10 174 1.44e-33 119
Msa0821460 MtrunA17_Chr2g0331691 34.595 185 114 3 1 178 1 185 3.11e-32 115
Msa0821460 MtrunA17_Chr4g0004661 35.632 174 105 3 12 178 66 239 7.33e-31 113
Msa0821460 MtrunA17_Chr3g0138101 32.948 173 110 4 12 181 15 184 1.26e-24 96.3
Msa0821460 MtrunA17_Chr3g0138111 32.948 173 110 4 12 181 15 184 1.46e-24 95.9
Msa0821460 MtrunA17_Chr3g0138121 32.948 173 110 4 12 181 15 184 1.63e-24 95.9
Msa0821460 MtrunA17_Chr2g0331841 62.712 59 22 0 78 136 1 59 3.66e-22 86.3
Msa0821460 MtrunA17_Chr6g0465471 32.500 160 101 4 27 179 7 166 1.04e-20 85.5
Msa0821460 MtrunA17_Chr6g0466161 33.333 150 93 4 27 169 7 156 1.43e-20 85.1
Msa0821460 MtrunA17_Chr3g0117931 32.335 167 95 6 12 164 10 172 3.59e-20 84.3
Msa0821460 MtrunA17_Chr8g0371501 32.121 165 98 4 12 164 8 170 6.13e-20 83.2
Msa0821460 MtrunA17_Chr5g0405791 31.515 165 99 4 12 164 8 170 3.22e-19 81.3
Msa0821460 MtrunA17_Chr6g0486041 30.539 167 98 5 12 164 8 170 1.35e-18 80.1
Msa0821460 MtrunA17_Chr4g0037021 30.303 165 101 4 12 164 8 170 1.80e-18 79.3
Msa0821460 MtrunA17_Chr6g0466431 37.113 97 52 1 12 108 10 97 2.45e-18 76.6
Msa0821460 MtrunA17_Chr6g0465611 37.113 97 52 1 12 108 10 97 2.45e-18 76.6
Msa0821460 MtrunA17_Chr4g0046211 32.787 122 79 3 12 132 8 127 3.70e-18 77.4
Msa0821460 MtrunA17_Chr2g0325101 29.091 165 103 4 12 164 8 170 6.58e-18 77.8
Msa0821460 MtrunA17_Chr2g0331831 46.154 65 34 1 136 200 2 65 8.88e-15 66.2
Msa0821460 MtrunA17_Chr5g0443631 30.588 170 108 4 9 171 103 269 9.02e-15 70.9
Msa0821460 MtrunA17_Chr4g0024431 30.000 170 109 4 9 171 90 256 1.44e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821460 AT5G45130.1 80.000 200 40 0 1 200 1 200 2.20e-119 337
Msa0821460 AT4G19640.1 78.000 200 44 0 1 200 1 200 1.62e-117 332
Msa0821460 AT5G45130.2 81.283 187 35 0 14 200 22 208 1.54e-112 320
Msa0821460 AT4G19640.2 83.571 140 23 0 1 140 1 140 7.79e-83 242
Msa0821460 AT4G19640.3 81.690 142 26 0 1 142 1 142 1.06e-82 244
Msa0821460 AT3G54840.1 63.158 171 62 1 8 177 31 201 1.68e-77 231
Msa0821460 AT3G54840.3 63.253 166 60 1 13 177 2 167 1.92e-75 224
Msa0821460 AT3G54840.2 63.924 158 56 1 8 164 31 188 1.78e-71 215
Msa0821460 AT5G60860.1 39.109 202 110 2 12 200 15 216 1.68e-49 160
Msa0821460 AT1G07410.1 42.000 200 105 3 12 200 14 213 2.37e-49 160
Msa0821460 AT4G39990.1 38.835 206 107 4 12 200 19 222 2.70e-49 160
Msa0821460 AT4G18800.1 39.196 199 111 2 12 200 15 213 5.23e-49 159
Msa0821460 AT5G45750.1 38.806 201 111 2 12 200 15 215 1.38e-48 158
Msa0821460 AT2G44610.1 44.720 161 89 0 12 172 11 171 2.44e-48 157
Msa0821460 AT5G10260.1 43.478 161 91 0 12 172 11 171 2.53e-48 157
Msa0821460 AT2G22290.1 38.776 196 113 2 12 200 11 206 4.39e-48 156
Msa0821460 AT4G35860.1 44.375 160 89 0 12 171 8 167 4.43e-48 156
Msa0821460 AT1G16920.1 40.796 201 107 2 12 200 15 215 4.70e-48 156
Msa0821460 AT1G09630.1 46.914 162 86 0 12 173 14 175 7.72e-48 156
Msa0821460 AT4G17160.1 44.586 157 87 0 12 168 8 164 1.08e-47 155
Msa0821460 AT1G06400.1 40.299 201 108 2 12 200 15 215 1.49e-47 155
Msa0821460 AT4G18430.1 38.119 202 112 2 12 200 15 216 1.70e-47 155
Msa0821460 AT1G28550.1 43.210 162 92 0 12 173 15 176 5.24e-47 154
Msa0821460 AT3G07410.1 41.379 203 105 3 12 200 14 216 5.50e-47 154
Msa0821460 AT3G15060.1 43.827 162 91 0 12 173 15 176 7.95e-47 153
Msa0821460 AT4G39890.1 42.593 162 92 1 12 172 11 172 8.39e-47 153
Msa0821460 AT5G59150.1 41.262 206 101 4 12 200 14 216 1.24e-46 153
Msa0821460 AT2G33870.1 43.827 162 91 0 12 173 15 176 1.57e-46 152
Msa0821460 AT3G46830.1 40.291 206 104 3 12 200 14 217 1.85e-46 152
Msa0821460 AT3G46830.2 40.291 206 104 3 12 200 14 217 1.85e-46 152
Msa0821460 AT4G17530.1 40.206 194 109 3 12 200 10 201 2.04e-46 152
Msa0821460 AT5G47200.1 40.104 192 112 1 12 200 10 201 4.69e-46 151
Msa0821460 AT1G02130.1 40.513 195 108 3 12 200 10 202 5.63e-46 150
Msa0821460 AT5G47520.1 37.561 205 112 4 12 200 16 220 7.78e-46 151
Msa0821460 AT5G65270.1 42.500 160 92 0 12 171 19 178 1.23e-45 150
Msa0821460 AT4G17170.1 42.675 157 90 0 12 168 8 164 1.99e-45 149
Msa0821460 AT2G43130.1 38.500 200 112 2 12 200 14 213 5.21e-45 149
Msa0821460 AT3G12160.1 43.827 162 91 0 12 173 17 178 6.91e-45 149
Msa0821460 AT5G59840.1 38.500 200 109 4 12 199 17 214 3.48e-44 147
Msa0821460 AT3G11730.1 37.113 194 115 1 12 198 10 203 7.59e-44 145
Msa0821460 AT5G47960.1 41.667 168 98 0 12 179 17 184 9.33e-44 145
Msa0821460 AT1G05810.1 36.946 203 114 3 12 200 14 216 2.32e-43 144
Msa0821460 AT3G46060.2 37.500 200 111 4 12 199 17 214 3.72e-43 144
Msa0821460 AT3G46060.3 37.500 200 111 4 12 199 17 214 3.72e-43 144
Msa0821460 AT3G46060.1 37.500 200 111 4 12 199 17 214 3.72e-43 144
Msa0821460 AT5G64990.1 40.476 168 100 0 4 171 1 168 4.92e-43 143
Msa0821460 AT1G05810.2 36.946 203 114 3 12 200 60 262 5.40e-43 145
Msa0821460 AT3G53610.2 36.453 203 109 4 12 199 17 214 1.47e-42 142
Msa0821460 AT3G53610.3 36.453 203 109 4 12 199 17 214 1.47e-42 142
Msa0821460 AT3G53610.1 36.453 203 109 4 12 199 17 214 1.47e-42 142
Msa0821460 AT1G73640.1 45.098 153 84 0 12 164 15 167 8.05e-42 141
Msa0821460 AT2G31680.1 35.784 204 116 3 12 200 14 217 1.88e-41 140
Msa0821460 AT1G18200.1 42.500 160 92 0 12 171 15 174 3.20e-41 139
Msa0821460 AT1G01200.1 42.236 161 92 1 12 171 30 190 2.62e-40 137
Msa0821460 AT5G64990.2 38.857 175 100 1 4 171 1 175 3.08e-40 136
Msa0821460 AT5G03520.1 38.384 198 113 4 12 200 17 214 8.27e-40 135
Msa0821460 AT3G09900.1 37.000 200 115 3 12 200 17 216 2.12e-39 134
Msa0821460 AT4G35860.2 46.667 120 64 0 52 171 2 121 3.98e-37 127
Msa0821460 AT1G22740.1 40.000 165 92 3 12 169 10 174 1.13e-36 127
Msa0821460 AT5G03530.2 38.614 202 113 6 8 200 11 210 4.88e-36 125
Msa0821460 AT5G03530.1 38.614 202 113 6 8 200 11 210 4.88e-36 125
Msa0821460 AT1G43890.3 40.741 162 93 3 12 171 15 175 3.69e-35 123
Msa0821460 AT1G43890.1 40.741 162 93 3 12 171 15 175 3.69e-35 123
Msa0821460 AT1G43890.2 40.741 162 93 3 12 171 15 175 3.69e-35 123
Msa0821460 AT3G18820.1 37.576 165 96 3 12 169 10 174 2.43e-33 119
Msa0821460 AT1G52280.1 35.795 176 106 3 12 180 10 185 2.79e-33 118
Msa0821460 AT2G21880.1 38.415 164 94 3 12 168 11 174 3.06e-33 118
Msa0821460 AT5G59150.2 40.361 166 79 4 52 200 3 165 7.27e-33 116
Msa0821460 AT4G09720.1 39.773 176 97 4 1 169 1 174 1.72e-32 116
Msa0821460 AT3G16100.1 36.207 174 104 3 12 178 10 183 2.70e-32 116
Msa0821460 AT5G39620.1 38.554 166 95 4 12 170 7 172 2.82e-32 115
Msa0821460 AT1G49300.2 37.423 163 95 3 12 167 10 172 2.95e-31 113
Msa0821460 AT1G49300.1 37.423 163 95 3 12 167 10 172 2.95e-31 113
Msa0821460 AT5G03520.2 36.196 163 95 4 47 200 42 204 1.32e-30 111
Msa0821460 AT3G09910.3 35.897 195 122 3 8 200 11 204 2.28e-30 111
Msa0821460 AT3G09910.1 35.897 195 122 3 8 200 11 204 2.28e-30 111
Msa0821460 AT3G09910.2 35.897 195 122 3 8 200 11 204 2.28e-30 111
Msa0821460 AT4G09720.4 38.674 181 97 5 1 169 1 179 8.97e-30 109
Msa0821460 AT4G09720.3 37.433 187 97 5 1 169 1 185 5.61e-29 107
Msa0821460 AT3G09910.5 39.286 168 99 3 8 173 11 177 1.22e-28 106
Msa0821460 AT3G09910.4 39.286 168 99 3 8 173 11 177 1.22e-28 106
Msa0821460 AT2G21880.4 36.735 147 86 3 29 168 3 149 8.97e-26 98.6
Msa0821460 AT2G21880.3 36.735 147 86 3 29 168 3 149 8.97e-26 98.6
Msa0821460 AT5G55080.1 30.625 160 108 3 12 171 15 171 4.30e-25 97.8
Msa0821460 AT4G09720.6 39.416 137 76 3 40 169 4 140 1.40e-24 95.1
Msa0821460 AT4G09720.5 39.416 137 76 3 40 169 4 140 1.40e-24 95.1
Msa0821460 AT4G09720.2 39.416 137 76 3 40 169 4 140 1.40e-24 95.1
Msa0821460 AT5G55190.1 33.750 160 103 3 12 171 15 171 3.08e-24 95.5
Msa0821460 AT5G20020.1 33.750 160 103 3 12 171 15 171 3.81e-24 95.5
Msa0821460 AT5G20010.1 33.750 160 103 3 12 171 15 171 3.89e-24 95.5
Msa0821460 AT2G21880.2 33.537 164 94 4 12 168 11 166 1.98e-23 93.2
Msa0821460 AT3G51300.1 31.928 166 99 4 11 164 7 170 2.66e-20 84.7
Msa0821460 AT2G44690.1 32.941 170 100 5 12 169 20 187 5.94e-20 84.0
Msa0821460 AT2G17800.2 32.727 165 97 4 12 164 8 170 6.50e-20 83.6
Msa0821460 AT2G17800.1 32.727 165 97 4 12 164 8 170 6.50e-20 83.6
Msa0821460 AT4G28950.1 31.515 165 97 5 12 164 8 168 7.80e-20 83.6
Msa0821460 AT4G35950.1 32.727 165 97 5 12 164 8 170 2.11e-19 82.4
Msa0821460 AT3G48040.1 29.940 167 99 5 12 164 10 172 8.08e-19 80.9
Msa0821460 AT4G35020.1 32.121 165 98 5 12 164 8 170 1.36e-18 80.1
Msa0821460 AT4G35020.2 32.121 165 98 5 12 164 8 170 1.36e-18 80.1
Msa0821460 AT4G35020.3 32.121 165 98 5 12 164 8 170 1.36e-18 80.1
Msa0821460 AT5G62880.1 31.737 167 96 6 12 164 10 172 2.97e-18 79.7
Msa0821460 AT1G75840.1 30.909 165 100 5 12 164 8 170 5.56e-18 78.6
Msa0821460 AT1G20090.1 30.909 165 100 5 12 164 7 169 7.38e-18 78.2
Msa0821460 AT5G46025.1 38.710 93 56 1 80 171 14 106 1.34e-17 75.5
Msa0821460 AT5G45970.1 29.697 165 102 4 12 164 8 170 1.36e-17 77.8
Msa0821460 AT3G48040.2 29.560 159 94 5 12 156 10 164 9.19e-17 74.7
Msa0821460 AT5G62880.2 31.447 159 91 6 12 156 10 164 3.11e-16 73.9
Msa0821460 AT3G51290.2 34.109 129 72 3 48 164 644 771 9.04e-16 75.5
Msa0821460 AT4G35020.4 32.456 114 74 3 12 124 8 119 9.59e-13 63.5
Msa0821460 AT4G35020.5 32.456 114 74 3 12 124 8 119 9.59e-13 63.5
Msa0821460 AT3G21700.3 28.655 171 111 5 9 171 105 272 1.21e-11 62.8
Msa0821460 AT5G09910.1 27.485 171 87 5 12 148 24 191 9.58e-11 60.5
Msa0821460 AT5G09910.4 27.485 171 87 5 12 148 24 191 9.58e-11 60.5

Find 66 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTCAATTCCTTGAATTTC+AGG 0.172043 5_3:+59609979 Msa0821460:CDS
GCGAAGCTCCGACAAGTTTA+TGG 0.180216 5_3:+59614726 Msa0821460:three_prime_UTR
TCAGGTTCTTCTTGGTGATA+TGG 0.216552 5_3:+59609919 Msa0821460:intron
CGTAGCCATTGAGTTGAAAT+TGG 0.254275 5_3:-59609772 None:intergenic
CCACAGTGAAATTTGAGATT+TGG 0.262162 5_3:+59611348 Msa0821460:CDS
TTGTGCCCTTGGTAACTTCT+TGG 0.276630 5_3:-59614541 None:intergenic
ATTCAATTTCAGGTTCTTCT+TGG 0.293743 5_3:+59609911 Msa0821460:intron
CACAGTGAAATTTGAGATTT+GGG 0.299158 5_3:+59611349 Msa0821460:CDS
CGTCATTTACTGCCAACGTC+TGG 0.316274 5_3:-59611325 None:intergenic
CACCAACCTCAACGCCAAAT+TGG 0.317821 5_3:+59609803 Msa0821460:CDS
TGATGATCTGTTATGTATAT+AGG 0.331167 5_3:+59614813 Msa0821460:three_prime_UTR
CAGGTTCTTCTTGGTGATAT+GGG 0.343041 5_3:+59609920 Msa0821460:intron
GTGAAACAGGAATCAACAAT+AGG 0.375057 5_3:+59611287 Msa0821460:intron
CATTTACACGAGCGAAGAAA+TGG 0.391385 5_3:+59611680 Msa0821460:CDS
CTGGTTTGAATTATATCCCT+TGG 0.395052 5_3:+59614774 Msa0821460:three_prime_UTR
CGTGTATATGCTGAGGAAAA+TGG 0.414206 5_3:+59613553 Msa0821460:CDS
CGAAGCTCCGACAAGTTTAT+GGG 0.414908 5_3:+59614727 Msa0821460:three_prime_UTR
GAAGAAATTGAAATCGGAAT+AGG 0.415842 5_3:-59609706 None:intergenic
GATGATCTGTTATGTATATA+GGG 0.419177 5_3:+59614814 Msa0821460:three_prime_UTR
CTAGATGAAGAAATTGAAAT+CGG 0.429423 5_3:-59609712 None:intergenic
GCAAAAGCCACGACCATGTT+CGG 0.437301 5_3:-59612265 None:intergenic
TGAAACAGGAATCAACAATA+GGG 0.443425 5_3:+59611288 Msa0821460:intron
TACCTTGCTTCTGAAGCTCT+TGG 0.443949 5_3:-59611705 None:intergenic
CAACCAGCGCAAAACCCAGC+AGG 0.448579 5_3:+59614561 Msa0821460:CDS
CTTCTTGGTGATATGGGTGC+TGG 0.450654 5_3:+59609926 Msa0821460:CDS
TCGTTCCAATTTCAACTCAA+TGG 0.452662 5_3:+59609767 Msa0821460:exon
AATGACATAATGTAACTTGC+TGG 0.453861 5_3:+59614755 Msa0821460:three_prime_UTR
GCAGCTCTGGTTCCTTCGGC+AGG 0.470619 5_3:-59614603 None:intergenic
TGTCTATGATATTACTAACT+TGG 0.471350 5_3:+59611442 Msa0821460:CDS
TGCTGGTAACAAAGCTGACT+TGG 0.472663 5_3:+59612285 Msa0821460:CDS
ATAAAATATGTCATTAACAT+TGG 0.476631 5_3:-59613611 None:intergenic
TTTGCAGCCCATAAACTTGT+CGG 0.476854 5_3:-59614734 None:intergenic
TCAACAAGAACCATCCCTGC+TGG 0.485332 5_3:-59614576 None:intergenic
TGAACAACATGATGCAGCTC+TGG 0.499235 5_3:-59614616 None:intergenic
TGAGTTGAAATTGGAACGAT+CGG 0.502735 5_3:-59609763 None:intergenic
AGAATAAAATTCAACATCCA+AGG 0.510030 5_3:-59614791 None:intergenic
AAGCTGACTTGGAAGATAAG+AGG 0.510951 5_3:+59612296 Msa0821460:CDS
ACAGAAAACGCGTTTAACTA+AGG 0.524264 5_3:-59614663 None:intergenic
TGATGCAGCTCTGGTTCCTT+CGG 0.524363 5_3:-59614607 None:intergenic
GTCATTTACTGCCAACGTCT+GGG 0.532033 5_3:-59611324 None:intergenic
TTAGCTCCCATGTACTATAG+AGG 0.537219 5_3:+59611401 Msa0821460:CDS
TTTGAGATTTGGGACACAGC+AGG 0.538081 5_3:+59611359 Msa0821460:CDS
AGCGCAAAACCCAGCAGGGA+TGG 0.543975 5_3:+59614566 Msa0821460:CDS
GAGATACCAATTTGGCGTTG+AGG 0.547489 5_3:-59609809 None:intergenic
TACCAATTTGGCGTTGAGGT+TGG 0.554563 5_3:-59609805 None:intergenic
ATTTACACGAGCGAAGAAAT+GGG 0.562436 5_3:+59611681 Msa0821460:CDS
AGCAGCGCCTCTATAGTACA+TGG 0.565513 5_3:-59611408 None:intergenic
CAACAAGAACCATCCCTGCT+GGG 0.567898 5_3:-59614575 None:intergenic
CAACTTCAATAAGCAAAATG+TGG 0.578284 5_3:+59614906 Msa0821460:three_prime_UTR
AGGGAATCCGAACATGGTCG+TGG 0.578957 5_3:+59612258 Msa0821460:intron
TGTTGCAGGGAATCCGAACA+TGG 0.580245 5_3:+59612252 Msa0821460:intron
AACCAGCGCAAAACCCAGCA+GGG 0.586823 5_3:+59614562 Msa0821460:CDS
CCTTAACAAAACGCAAAACA+AGG 0.590364 5_3:-59609958 None:intergenic
TTCAACTCAATGGCTACGAT+CGG 0.593200 5_3:+59609777 Msa0821460:exon
TGAAATTGGAACGATCGGAA+TGG 0.594091 5_3:-59609758 None:intergenic
AGTACATGGGAGCTAAGCTA+TGG 0.598496 5_3:-59611394 None:intergenic
GTCCAAGAGCTTCAGAAGCA+AGG 0.599811 5_3:+59611703 Msa0821460:CDS
GAATAAAATTCAACATCCAA+GGG 0.604452 5_3:-59614790 None:intergenic
GAAAACGCGTTTAACTAAGG+TGG 0.612782 5_3:-59614660 None:intergenic
CTTGTTGATAGACCTGCCGA+AGG 0.652869 5_3:+59614591 Msa0821460:CDS
GGAAGCACGTGTATATGCTG+AGG 0.658380 5_3:+59613546 Msa0821460:intron
GAAACAGGAATCAACAATAG+GGG 0.660375 5_3:+59611289 Msa0821460:intron
GAACAGCCAAGAAGTTACCA+AGG 0.663916 5_3:+59614535 Msa0821460:intron
AACAGCCAAGAAGTTACCAA+GGG 0.689870 5_3:+59614536 Msa0821460:intron
GCAGCGCCTCTATAGTACAT+GGG 0.698335 5_3:-59611407 None:intergenic
CCAAATCTCAAATTTCACTG+TGG 0.747675 5_3:-59611348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAAAGATAAATCAAA+AGG - chr5_3:59610013-59610032 None:intergenic 10.0%
!!! ATTTATCTTTTAAATTCAAT+TGG + chr5_3:59610017-59610036 Msa0821460:intron 10.0%
!!! TTTATCTTTTAAATTCAATT+GGG + chr5_3:59610018-59610037 Msa0821460:intron 10.0%
!!! AAGAAAAAACAATTTTATTT+AGG - chr5_3:59610536-59610555 None:intergenic 10.0%
!!! AAAAATTTTAGATTGTAAAA+AGG + chr5_3:59611782-59611801 Msa0821460:intron 10.0%
!! ATATTAACCTTAATTCAATT+TGG + chr5_3:59609885-59609904 Msa0821460:intron 15.0%
!!! TTTTTGACTAAATTGAAATT+GGG + chr5_3:59610167-59610186 Msa0821460:intron 15.0%
!! TTATATATAGTTTATTACTG+TGG + chr5_3:59611617-59611636 Msa0821460:intron 15.0%
!! TAAGAAGAAATATTAGAATA+TGG - chr5_3:59611871-59611890 None:intergenic 15.0%
!! ATTAATATATGAATTGTGAA+CGG + chr5_3:59612012-59612031 Msa0821460:intron 15.0%
!! AAACAATAGCTAAAAAATTT+CGG - chr5_3:59612128-59612147 None:intergenic 15.0%
!! ATAAAATATGTCATTAACAT+TGG - chr5_3:59613614-59613633 None:intergenic 15.0%
!!! AATGACATATTTTATGAAAT+AGG + chr5_3:59613619-59613638 Msa0821460:CDS 15.0%
!! ATGATATTTAAGTGTAATAA+TGG + chr5_3:59614391-59614410 Msa0821460:intron 15.0%
!! GAATAAACCAAATTGAATTA+AGG - chr5_3:59609895-59609914 None:intergenic 20.0%
!!! AATTTGGTTTATTCAATTTC+AGG + chr5_3:59609901-59609920 Msa0821460:intron 20.0%
!! TGTAGAATTCAAAATTTATG+AGG + chr5_3:59610045-59610064 Msa0821460:intron 20.0%
!!! GTTTTTGACTAAATTGAAAT+TGG + chr5_3:59610166-59610185 Msa0821460:intron 20.0%
!!! TTTTGACTAAATTGAAATTG+GGG + chr5_3:59610168-59610187 Msa0821460:intron 20.0%
!! ATCTTATCTTATAGAATGTA+TGG + chr5_3:59610383-59610402 Msa0821460:intron 20.0%
!!! TCATAGCATGTTATATTTTT+TGG + chr5_3:59610757-59610776 Msa0821460:intron 20.0%
!!! CATAGCATGTTATATTTTTT+GGG + chr5_3:59610758-59610777 Msa0821460:intron 20.0%
!! TTTACTTAAAAGTTCAAACT+GGG + chr5_3:59610825-59610844 Msa0821460:intron 20.0%
!!! TTATTTTGTATATAGTAGTG+TGG + chr5_3:59611161-59611180 Msa0821460:intron 20.0%
!!! TATTTTGTATATAGTAGTGT+GGG + chr5_3:59611162-59611181 Msa0821460:intron 20.0%
!!! TCTTATTCTTACATTTTTTC+TGG + chr5_3:59611751-59611770 Msa0821460:intron 20.0%
!! GCATAAGAATAAATCATATA+AGG + chr5_3:59613719-59613738 Msa0821460:intron 20.0%
!!! TAAGATGGTATTTTCAAATT+TGG + chr5_3:59614367-59614386 Msa0821460:intron 20.0%
!! AAATTCATTCACACAAATAA+TGG + chr5_3:59614424-59614443 Msa0821460:intron 20.0%
!! TGTTGACAAAAATAAAACAA+CGG - chr5_3:59614868-59614887 None:intergenic 20.0%
! CTAGATGAAGAAATTGAAAT+CGG - chr5_3:59609715-59609734 None:intergenic 25.0%
! AAAAAAGAGAGATACCAATT+TGG - chr5_3:59609820-59609839 None:intergenic 25.0%
! GAATTGAATGAGAAAAAAAG+GGG - chr5_3:59609848-59609867 None:intergenic 25.0%
! CGAATTGAATGAGAAAAAAA+GGG - chr5_3:59609849-59609868 None:intergenic 25.0%
! TCGAATTGAATGAGAAAAAA+AGG - chr5_3:59609850-59609869 None:intergenic 25.0%
! GAAAAAAAACCTGAAATTCA+AGG - chr5_3:59609991-59610010 None:intergenic 25.0%
!! AGAATGAATTGTTATGGTTT+TGG + chr5_3:59610290-59610309 Msa0821460:intron 25.0%
! TTATCTTATAGAATGTATGG+TGG + chr5_3:59610386-59610405 Msa0821460:intron 25.0%
! TTGAGAAATGTTGTTCAATT+AGG + chr5_3:59610692-59610711 Msa0821460:intron 25.0%
! TGAGAAATGTTGTTCAATTA+GGG + chr5_3:59610693-59610712 Msa0821460:intron 25.0%
! CTTTACTTAAAAGTTCAAAC+TGG + chr5_3:59610824-59610843 Msa0821460:intron 25.0%
! TGAGTTATCAAACATTCATT+TGG + chr5_3:59610858-59610877 Msa0821460:intron 25.0%
! CAATAAATAACAACAGATCT+CGG - chr5_3:59611208-59611227 None:intergenic 25.0%
! AGTCTATCTGTTTAAAGAAT+TGG - chr5_3:59611231-59611250 None:intergenic 25.0%
! TGTCTATGATATTACTAACT+TGG + chr5_3:59611442-59611461 Msa0821460:CDS 25.0%
! AACTAACCCATGATATTTAA+TGG + chr5_3:59612099-59612118 Msa0821460:intron 25.0%
! TTTCCTATACATGTTACAAA+AGG + chr5_3:59612145-59612164 Msa0821460:intron 25.0%
! ATTGTACTTCAAAGTTATAG+TGG + chr5_3:59612184-59612203 Msa0821460:intron 25.0%
! CTAAAATAATGAACTCATTC+TGG + chr5_3:59612226-59612245 Msa0821460:intron 25.0%
! AATTTAGCAGAAAAAATGTG+TGG - chr5_3:59612431-59612450 None:intergenic 25.0%
!! TGCTAAATTTTCAAATACCA+GGG + chr5_3:59612442-59612461 Msa0821460:intron 25.0%
!! ATTAAGAATTTAGTTCCAAC+TGG + chr5_3:59612867-59612886 Msa0821460:intron 25.0%
! AAGAGCTTCTTAAGAAATTA+AGG + chr5_3:59612938-59612957 Msa0821460:intron 25.0%
! TTATAAGAGAAGCTATTAGT+TGG + chr5_3:59612980-59612999 Msa0821460:intron 25.0%
! TATAAACTCCTTGAATAAAG+TGG + chr5_3:59613157-59613176 Msa0821460:intron 25.0%
! ATAAACTCCTTGAATAAAGT+GGG + chr5_3:59613158-59613177 Msa0821460:intron 25.0%
! GCAACTGTTTAAATACATAT+AGG - chr5_3:59613446-59613465 None:intergenic 25.0%
! TAAAGTGTATGTAATAGGAA+AGG - chr5_3:59613652-59613671 None:intergenic 25.0%
! TTTCCTATTACATACACTTT+AGG + chr5_3:59613651-59613670 Msa0821460:intron 25.0%
! ACATAATTTATATCAGTCCA+AGG + chr5_3:59613746-59613765 Msa0821460:intron 25.0%
!! TGCAAACAACACAAATATTT+TGG - chr5_3:59613818-59613837 None:intergenic 25.0%
! GGTTAGATAAATGTACATAA+AGG + chr5_3:59613843-59613862 Msa0821460:intron 25.0%
!! GAATTTTATTATTCCTAGTC+TGG + chr5_3:59613881-59613900 Msa0821460:intron 25.0%
!! TAGTCTTAAGTAAGAGAAAA+TGG + chr5_3:59614318-59614337 Msa0821460:intron 25.0%
! ATTGTACTCTCAATATAAGA+TGG + chr5_3:59614352-59614371 Msa0821460:intron 25.0%
!!! TTTTTTAAATACATCGAGAG+AGG - chr5_3:59614462-59614481 None:intergenic 25.0%
! GAATAAAATTCAACATCCAA+GGG - chr5_3:59614793-59614812 None:intergenic 25.0%
! AGAATAAAATTCAACATCCA+AGG - chr5_3:59614794-59614813 None:intergenic 25.0%
! TGATGATCTGTTATGTATAT+AGG + chr5_3:59614813-59614832 Msa0821460:three_prime_UTR 25.0%
! GATGATCTGTTATGTATATA+GGG + chr5_3:59614814-59614833 Msa0821460:three_prime_UTR 25.0%
! GTTGACAAAAATAAAACAAC+GGG - chr5_3:59614867-59614886 None:intergenic 25.0%
! ACAAGAAAAGACTCTGTTTT+CGG + chr5_3:59609668-59609687 Msa0821460:five_prime_UTR 30.0%
GAAGAAATTGAAATCGGAAT+AGG - chr5_3:59609709-59609728 None:intergenic 30.0%
!! ATTCAATTTCAGGTTCTTCT+TGG + chr5_3:59609911-59609930 Msa0821460:intron 30.0%
!!! CTTGTTTTGCGTTTTGTTAA+GGG + chr5_3:59609959-59609978 Msa0821460:CDS 30.0%
ATTTATGAGGACAATTTCCA+TGG + chr5_3:59610058-59610077 Msa0821460:intron 30.0%
TTTATGAGGACAATTTCCAT+GGG + chr5_3:59610059-59610078 Msa0821460:intron 30.0%
CTCGATTACAAAATCATAAC+AGG - chr5_3:59610198-59610217 None:intergenic 30.0%
AGTTCTATTCTGAACAAGAT+AGG + chr5_3:59610258-59610277 Msa0821460:intron 30.0%
GTTGTGAGAATGAATTGTTA+TGG + chr5_3:59610284-59610303 Msa0821460:intron 30.0%
!!! ATGAATTGTTATGGTTTTGG+AGG + chr5_3:59610293-59610312 Msa0821460:intron 30.0%
GAGAAATGTTGTTCAATTAG+GGG + chr5_3:59610694-59610713 Msa0821460:intron 30.0%
AGAAATGTTGTTCAATTAGG+GGG + chr5_3:59610695-59610714 Msa0821460:intron 30.0%
ATATAACATGCTATGAAGCA+TGG - chr5_3:59610753-59610772 None:intergenic 30.0%
!!! GCATGTTATATTTTTTGGGT+TGG + chr5_3:59610762-59610781 Msa0821460:intron 30.0%
ATTCACCTAACCAAGTTATT+TGG + chr5_3:59610919-59610938 Msa0821460:intron 30.0%
TTCACCTAACCAAGTTATTT+GGG + chr5_3:59610920-59610939 Msa0821460:intron 30.0%
TTAACCCAAATAACTTGGTT+AGG - chr5_3:59610927-59610946 None:intergenic 30.0%
CAACTTTAACCCAAATAACT+TGG - chr5_3:59610932-59610951 None:intergenic 30.0%
TTAAAGTTGTTCATGAACTC+CGG + chr5_3:59610943-59610962 Msa0821460:intron 30.0%
GCCAAAAAAAATTTATCTCC+CGG + chr5_3:59611022-59611041 Msa0821460:intron 30.0%
!!! ACCGGGAGATAAATTTTTTT+TGG - chr5_3:59611026-59611045 None:intergenic 30.0%
!!! TTATTTTTGTCTTAACCCTC+CGG - chr5_3:59611084-59611103 None:intergenic 30.0%
TCTTTAAACAGATAGACTTG+TGG + chr5_3:59611233-59611252 Msa0821460:intron 30.0%
CTTTAAACAGATAGACTTGT+GGG + chr5_3:59611234-59611253 Msa0821460:intron 30.0%
TATTTGGTCTATTGTGAAAC+AGG + chr5_3:59611274-59611293 Msa0821460:intron 30.0%
TGAAACAGGAATCAACAATA+GGG + chr5_3:59611288-59611307 Msa0821460:intron 30.0%
! CACAGTGAAATTTGAGATTT+GGG + chr5_3:59611349-59611368 Msa0821460:CDS 30.0%
!!! TGTGTCATTTTCCTTTCTTT+AGG + chr5_3:59611546-59611565 Msa0821460:intron 30.0%
!!! GTGTCATTTTCCTTTCTTTA+GGG + chr5_3:59611547-59611566 Msa0821460:intron 30.0%
! TGATTATTTCTTCTGTTCAC+AGG + chr5_3:59611654-59611673 Msa0821460:intron 30.0%
AATATAGCATAGCACAAACA+TGG + chr5_3:59611957-59611976 Msa0821460:intron 30.0%
AAATTAACGCAAAGTTCAGT+AGG - chr5_3:59612077-59612096 None:intergenic 30.0%
CGGTAACCATTAAATATCAT+GGG - chr5_3:59612108-59612127 None:intergenic 30.0%
TCGGTAACCATTAAATATCA+TGG - chr5_3:59612109-59612128 None:intergenic 30.0%
AGTACAATTACAACCAGAAT+GGG - chr5_3:59612172-59612191 None:intergenic 30.0%
AAGTACAATTACAACCAGAA+TGG - chr5_3:59612173-59612192 None:intergenic 30.0%
! CTGCTAAATTTTCAAATACC+AGG + chr5_3:59612441-59612460 Msa0821460:intron 30.0%
! AAATTTTCAAATACCAGGGA+TGG + chr5_3:59612446-59612465 Msa0821460:intron 30.0%
!! CATGTTGATAGTTCTACTAT+CGG + chr5_3:59613098-59613117 Msa0821460:intron 30.0%
! AGCTTATGCGATTTTCATTT+TGG - chr5_3:59613251-59613270 None:intergenic 30.0%
TTCAATGTACAAGCTAAAGT+TGG - chr5_3:59613303-59613322 None:intergenic 30.0%
CAACCTAAAGTGTATGTAAT+AGG - chr5_3:59613657-59613676 None:intergenic 30.0%
! AAATGGAAACTCTTCTTTTG+AGG + chr5_3:59613787-59613806 Msa0821460:intron 30.0%
CATAAAAAAACACATTCGTC+TGG - chr5_3:59613962-59613981 None:intergenic 30.0%
! AATGACATAATGTAACTTGC+TGG + chr5_3:59614755-59614774 Msa0821460:three_prime_UTR 30.0%
CAACTTCAATAAGCAAAATG+TGG + chr5_3:59614906-59614925 Msa0821460:three_prime_UTR 30.0%
! TGAGTTGAAATTGGAACGAT+CGG - chr5_3:59609766-59609785 None:intergenic 35.0%
TCGTTCCAATTTCAACTCAA+TGG + chr5_3:59609767-59609786 Msa0821460:exon 35.0%
CCTTAACAAAACGCAAAACA+AGG - chr5_3:59609961-59609980 None:intergenic 35.0%
!!! CCTTGTTTTGCGTTTTGTTA+AGG + chr5_3:59609958-59609977 Msa0821460:CDS 35.0%
TTGGTTAAACCTAAAACCCA+TGG - chr5_3:59610078-59610097 None:intergenic 35.0%
!!! TATGGTTTTGGAGGTTTGAA+TGG + chr5_3:59610302-59610321 Msa0821460:intron 35.0%
!!! ATGGTTTTGGAGGTTTGAAT+GGG + chr5_3:59610303-59610322 Msa0821460:intron 35.0%
ACAGTTCGTATGAGTTACAT+AGG - chr5_3:59610591-59610610 None:intergenic 35.0%
ACTCTTGTATTTACAGTGTG+TGG - chr5_3:59610662-59610681 None:intergenic 35.0%
ACTGCGTTGCTGAATTTATT+TGG + chr5_3:59611258-59611277 Msa0821460:intron 35.0%
GTGAAACAGGAATCAACAAT+AGG + chr5_3:59611287-59611306 Msa0821460:intron 35.0%
GAAACAGGAATCAACAATAG+GGG + chr5_3:59611289-59611308 Msa0821460:intron 35.0%
CCAAATCTCAAATTTCACTG+TGG - chr5_3:59611351-59611370 None:intergenic 35.0%
! CCACAGTGAAATTTGAGATT+TGG + chr5_3:59611348-59611367 Msa0821460:CDS 35.0%
GCACTAAAATATTCCAAGCA+TGG - chr5_3:59611588-59611607 None:intergenic 35.0%
ATTTACACGAGCGAAGAAAT+GGG + chr5_3:59611681-59611700 Msa0821460:CDS 35.0%
!! GAAGCAAGGTATTTTCTGAT+TGG + chr5_3:59611717-59611736 Msa0821460:intron 35.0%
! GGTCCTTTTGTAACATGTAT+AGG - chr5_3:59612151-59612170 None:intergenic 35.0%
TCAGTTTCACTACACATCAT+AGG + chr5_3:59612358-59612377 Msa0821460:intron 35.0%
TTATCAAATGAAACCATCCC+TGG - chr5_3:59612462-59612481 None:intergenic 35.0%
! ACTTTTATGCTGCTACGTTT+TGG + chr5_3:59612481-59612500 Msa0821460:intron 35.0%
! CTTTTATGCTGCTACGTTTT+GGG + chr5_3:59612482-59612501 Msa0821460:intron 35.0%
TACACAAAGCTATAGAGACT+GGG - chr5_3:59612525-59612544 None:intergenic 35.0%
ATACACAAAGCTATAGAGAC+TGG - chr5_3:59612526-59612545 None:intergenic 35.0%
! CTTTTCTAACCATAACCAAC+CGG + chr5_3:59612552-59612571 Msa0821460:intron 35.0%
AATCCTTTGTACAACCTGAT+TGG + chr5_3:59612623-59612642 Msa0821460:intron 35.0%
ATCCTTTGTACAACCTGATT+GGG + chr5_3:59612624-59612643 Msa0821460:intron 35.0%
AGAACATAAATGTCCCAATC+AGG - chr5_3:59612640-59612659 None:intergenic 35.0%
ACTTGAAGAAGCTCAGATAT+AGG - chr5_3:59612747-59612766 None:intergenic 35.0%
ATGAGAAACTACTTGTTTCG+AGG + chr5_3:59612801-59612820 Msa0821460:intron 35.0%
ATACACTTACTACGTCACAA+GGG - chr5_3:59612898-59612917 None:intergenic 35.0%
TATACACTTACTACGTCACA+AGG - chr5_3:59612899-59612918 None:intergenic 35.0%
TTGTGACGTAGTAAGTGTAT+AGG + chr5_3:59612898-59612917 Msa0821460:intron 35.0%
! CCTTTTCAATAAGCAGTTGT+AGG - chr5_3:59613054-59613073 None:intergenic 35.0%
CCTACAACTGCTTATTGAAA+AGG + chr5_3:59613051-59613070 Msa0821460:intron 35.0%
AGAATCTTCTGTGTTGGAAA+TGG - chr5_3:59613369-59613388 None:intergenic 35.0%
ACTTGAAGAATCTTCTGTGT+TGG - chr5_3:59613375-59613394 None:intergenic 35.0%
CATAAATGTAACTGGCACAT+CGG - chr5_3:59613484-59613503 None:intergenic 35.0%
GATGTGCCAGTTACATTTAT+GGG + chr5_3:59613483-59613502 Msa0821460:intron 35.0%
GTAGTTCCCATAAATGTAAC+TGG - chr5_3:59613492-59613511 None:intergenic 35.0%
TTTATATCAGTCCAAGGAAG+AGG + chr5_3:59613752-59613771 Msa0821460:intron 35.0%
!! ATTTGTGTTGTTTGCACTAC+TGG + chr5_3:59613822-59613841 Msa0821460:intron 35.0%
TCTTCGTATAAGACTGAAGA+AGG + chr5_3:59614012-59614031 Msa0821460:intron 35.0%
CTTCGTATAAGACTGAAGAA+GGG + chr5_3:59614013-59614032 Msa0821460:intron 35.0%
! GTTCACTCATGAACGTTTTA+TGG - chr5_3:59614055-59614074 None:intergenic 35.0%
!! TACAAGTTTTGTCACTCTGT+AGG + chr5_3:59614148-59614167 Msa0821460:intron 35.0%
!! ACAAGTTTTGTCACTCTGTA+GGG + chr5_3:59614149-59614168 Msa0821460:intron 35.0%
TCTCATTCTGTTTAGTGAGA+CGG - chr5_3:59614193-59614212 None:intergenic 35.0%
ACAGAAAACGCGTTTAACTA+AGG - chr5_3:59614666-59614685 None:intergenic 35.0%
! CTGGTTTGAATTATATCCCT+TGG + chr5_3:59614774-59614793 Msa0821460:three_prime_UTR 35.0%
TGAAATTGGAACGATCGGAA+TGG - chr5_3:59609761-59609780 None:intergenic 40.0%
!! CGTAGCCATTGAGTTGAAAT+TGG - chr5_3:59609775-59609794 None:intergenic 40.0%
TTCAACTCAATGGCTACGAT+CGG + chr5_3:59609777-59609796 Msa0821460:exon 40.0%
! TCAGGTTCTTCTTGGTGATA+TGG + chr5_3:59609919-59609938 Msa0821460:intron 40.0%
! CAGGTTCTTCTTGGTGATAT+GGG + chr5_3:59609920-59609939 Msa0821460:intron 40.0%
GGGTCAATTCCTTGAATTTC+AGG + chr5_3:59609979-59609998 Msa0821460:CDS 40.0%
! GGACAATTTCCATGGGTTTT+AGG + chr5_3:59610066-59610085 Msa0821460:intron 40.0%
AGAATGTATGGTGGTACTTG+TGG + chr5_3:59610395-59610414 Msa0821460:intron 40.0%
GAATGTATGGTGGTACTTGT+GGG + chr5_3:59610396-59610415 Msa0821460:intron 40.0%
TATGAAGCACAAACACCTCT+AGG + chr5_3:59610438-59610457 Msa0821460:intron 40.0%
CGTATGAGTTACATAGGTGT+CGG - chr5_3:59610585-59610604 None:intergenic 40.0%
GTAACTCATACGAACTGTGT+TGG + chr5_3:59610593-59610612 Msa0821460:intron 40.0%
CCAAATAGTTCGCTCTTAAC+CGG - chr5_3:59610965-59610984 None:intergenic 40.0%
AAGAATCCGAGTTTGAATCC+TGG + chr5_3:59610985-59611004 Msa0821460:intron 40.0%
! CATCTTTTCCTCGTAAGTAG+TGG - chr5_3:59611128-59611147 None:intergenic 40.0%
TTAGCTCCCATGTACTATAG+AGG + chr5_3:59611401-59611420 Msa0821460:CDS 40.0%
AATATTCCAAGCATGGTCTC+TGG - chr5_3:59611581-59611600 None:intergenic 40.0%
CATTTACACGAGCGAAGAAA+TGG + chr5_3:59611680-59611699 Msa0821460:CDS 40.0%
! GTTAAGCAGATTCGACTTGA+AGG + chr5_3:59612034-59612053 Msa0821460:intron 40.0%
TGTTACAAAAGGACCCATTC+TGG + chr5_3:59612156-59612175 Msa0821460:intron 40.0%
!!! ACTCATTCTGGTTTTGTTGC+AGG + chr5_3:59612238-59612257 Msa0821460:intron 40.0%
!!! CTCATTCTGGTTTTGTTGCA+GGG + chr5_3:59612239-59612258 Msa0821460:intron 40.0%
!! AAGCTGACTTGGAAGATAAG+AGG + chr5_3:59612296-59612315 Msa0821460:CDS 40.0%
! CGGTTGGTTATGGTTAGAAA+AGG - chr5_3:59612554-59612573 None:intergenic 40.0%
TCTTCCATGTTTGAAGATGC+CGG - chr5_3:59612574-59612593 None:intergenic 40.0%
! ACTTGTTTCGAGGCTCATTT+TGG + chr5_3:59612811-59612830 Msa0821460:intron 40.0%
!! TTGGACGAACTTCTAGCTTA+CGG + chr5_3:59612830-59612849 Msa0821460:intron 40.0%
TGACGTAGTAAGTGTATAGG+TGG + chr5_3:59612901-59612920 Msa0821460:intron 40.0%
GACGTAGTAAGTGTATAGGT+GGG + chr5_3:59612902-59612921 Msa0821460:intron 40.0%
AGTGTATAGGTGGGATTTGT+TGG + chr5_3:59612911-59612930 Msa0821460:intron 40.0%
AATCCAGCCCACTTTATTCA+AGG - chr5_3:59613168-59613187 None:intergenic 40.0%
! GAAATGGAGTACTAGGATCT+AGG - chr5_3:59613353-59613372 None:intergenic 40.0%
! TGTGTTGGAAATGGAGTACT+AGG - chr5_3:59613360-59613379 None:intergenic 40.0%
CGATGTGCCAGTTACATTTA+TGG + chr5_3:59613482-59613501 Msa0821460:intron 40.0%
ATTGTACAAGAGTCATGAGC+TGG - chr5_3:59613523-59613542 None:intergenic 40.0%
TCATGACTCTTGTACAATGC+AGG + chr5_3:59613525-59613544 Msa0821460:intron 40.0%
CGTGTATATGCTGAGGAAAA+TGG + chr5_3:59613553-59613572 Msa0821460:CDS 40.0%
AGAGGAAGAGGAAGAGAAAA+TGG + chr5_3:59613770-59613789 Msa0821460:intron 40.0%
TTCGTCTGGTGCAAATCAAA+AGG - chr5_3:59613948-59613967 None:intergenic 40.0%
AACAGCCAAGAAGTTACCAA+GGG + chr5_3:59614536-59614555 Msa0821460:intron 40.0%
GAAAACGCGTTTAACTAAGG+TGG - chr5_3:59614663-59614682 None:intergenic 40.0%
TTTGCAGCCCATAAACTTGT+CGG - chr5_3:59614737-59614756 None:intergenic 40.0%
! TGCAAACGCGCTTTTGATGT+AGG - chr5_3:59609611-59609630 None:intergenic 45.0%
! TACCAATTTGGCGTTGAGGT+TGG - chr5_3:59609808-59609827 None:intergenic 45.0%
GAGATACCAATTTGGCGTTG+AGG - chr5_3:59609812-59609831 None:intergenic 45.0%
! TACGAGTGTCATAGGAGTGT+CGG - chr5_3:59610483-59610502 None:intergenic 45.0%
ACGTGACATACGAGTGTCAT+AGG - chr5_3:59610491-59610510 None:intergenic 45.0%
TACTTCGACACTCCTAAGAC+AGG + chr5_3:59610556-59610575 Msa0821460:intron 45.0%
GTTCAATTAGGGGGTTGTAG+CGG + chr5_3:59610704-59610723 Msa0821460:intron 45.0%
CCGGTTAAGAGCGAACTATT+TGG + chr5_3:59610962-59610981 Msa0821460:intron 45.0%
! CTAGAGTACCACTACTTACG+AGG + chr5_3:59611117-59611136 Msa0821460:intron 45.0%
TAGTAGTGTGGGTGCATCAA+TGG + chr5_3:59611173-59611192 Msa0821460:intron 45.0%
! TGTGGGTGCATCAATGGTTT+TGG + chr5_3:59611179-59611198 Msa0821460:intron 45.0%
! GTGGGTGCATCAATGGTTTT+GGG + chr5_3:59611180-59611199 Msa0821460:intron 45.0%
! AGCATTTTTCTCCCAGACGT+TGG + chr5_3:59611313-59611332 Msa0821460:CDS 45.0%
GTCATTTACTGCCAACGTCT+GGG - chr5_3:59611327-59611346 None:intergenic 45.0%
! TTTGAGATTTGGGACACAGC+AGG + chr5_3:59611359-59611378 Msa0821460:CDS 45.0%
AGTACATGGGAGCTAAGCTA+TGG - chr5_3:59611397-59611416 None:intergenic 45.0%
!!! TTTTCCTTTCTTTAGGGCCC+TGG + chr5_3:59611553-59611572 Msa0821460:intron 45.0%
!! TACCTTGCTTCTGAAGCTCT+TGG - chr5_3:59611708-59611727 None:intergenic 45.0%
TGCTGGTAACAAAGCTGACT+TGG + chr5_3:59612285-59612304 Msa0821460:CDS 45.0%
!! TTATGCTGCTACGTTTTGGG+TGG + chr5_3:59612485-59612504 Msa0821460:intron 45.0%
! TTGAAGATGCCGGTTGGTTA+TGG - chr5_3:59612564-59612583 None:intergenic 45.0%
! GTCCCAATCAGGTTGTACAA+AGG - chr5_3:59612629-59612648 None:intergenic 45.0%
! AGTTCTACTATCGGTGCTTC+TGG + chr5_3:59613107-59613126 Msa0821460:intron 45.0%
! GTTCTACTATCGGTGCTTCT+GGG + chr5_3:59613108-59613127 Msa0821460:intron 45.0%
ACTCCTTGAATAAAGTGGGC+TGG + chr5_3:59613162-59613181 Msa0821460:intron 45.0%
GGGCTGGATTAATTCGTTGT+TGG + chr5_3:59613178-59613197 Msa0821460:intron 45.0%
CGAATCAAAGCAGCAATGCA+TGG - chr5_3:59613209-59613228 None:intergenic 45.0%
GAGTCATGAGCTGGATAAGT+TGG - chr5_3:59613514-59613533 None:intergenic 45.0%
CACTTCAGCAATACCAGACT+AGG - chr5_3:59613897-59613916 None:intergenic 45.0%
GTCTGGTATTGCTGAAGTGA+AGG + chr5_3:59613898-59613917 Msa0821460:intron 45.0%
GAACAGCCAAGAAGTTACCA+AGG + chr5_3:59614535-59614554 Msa0821460:intron 45.0%
! TTGTGCCCTTGGTAACTTCT+TGG - chr5_3:59614544-59614563 None:intergenic 45.0%
TGAACAACATGATGCAGCTC+TGG - chr5_3:59614619-59614638 None:intergenic 45.0%
CGAAGCTCCGACAAGTTTAT+GGG + chr5_3:59614727-59614746 Msa0821460:three_prime_UTR 45.0%
!!! AAAAAAAATCAATTTTTTTT+TGG - chr5_3:59610097-59610116 None:intergenic 5.0%
!!! TTTAAAATTTAATAATATGA+TGG + chr5_3:59610222-59610241 Msa0821460:intron 5.0%
CACCAACCTCAACGCCAAAT+TGG + chr5_3:59609803-59609822 Msa0821460:CDS 50.0%
! CTTCTTGGTGATATGGGTGC+TGG + chr5_3:59609926-59609945 Msa0821460:CDS 50.0%
!! GCACAAACACCTCTAGGACT+AGG + chr5_3:59610444-59610463 Msa0821460:intron 50.0%
GACACTCGTATGTCACGTGT+CGG + chr5_3:59610493-59610512 Msa0821460:intron 50.0%
ATGAAGCATGGACTCCGACA+CGG - chr5_3:59610741-59610760 None:intergenic 50.0%
ATCCGAGTTTGAATCCTGGC+TGG + chr5_3:59610989-59611008 Msa0821460:intron 50.0%
CTCCAGCCAGGATTCAAACT+CGG - chr5_3:59610994-59611013 None:intergenic 50.0%
TGGCTAAGAATACTCCAGCC+AGG - chr5_3:59611006-59611025 None:intergenic 50.0%
CATGGTAATCGAGTTCGACC+GGG - chr5_3:59611043-59611062 None:intergenic 50.0%
CCATGGTAATCGAGTTCGAC+CGG - chr5_3:59611044-59611063 None:intergenic 50.0%
CGGTCGAACTCGATTACCAT+GGG + chr5_3:59611042-59611061 Msa0821460:intron 50.0%
GGTCGAACTCGATTACCATG+GGG + chr5_3:59611043-59611062 Msa0821460:intron 50.0%
CGTCATTTACTGCCAACGTC+TGG - chr5_3:59611328-59611347 None:intergenic 50.0%
GCAGCGCCTCTATAGTACAT+GGG - chr5_3:59611410-59611429 None:intergenic 50.0%
AGCAGCGCCTCTATAGTACA+TGG - chr5_3:59611411-59611430 None:intergenic 50.0%
GTCCAAGAGCTTCAGAAGCA+AGG + chr5_3:59611703-59611722 Msa0821460:CDS 50.0%
TGTTGCAGGGAATCCGAACA+TGG + chr5_3:59612252-59612271 Msa0821460:intron 50.0%
GCAAAAGCCACGACCATGTT+CGG - chr5_3:59612268-59612287 None:intergenic 50.0%
! AACATGGTCGTGGCTTTTGC+TGG + chr5_3:59612268-59612287 Msa0821460:CDS 50.0%
CCATGTTTGAAGATGCCGGT+TGG - chr5_3:59612570-59612589 None:intergenic 50.0%
CCAACCGGCATCTTCAAACA+TGG + chr5_3:59612567-59612586 Msa0821460:intron 50.0%
! GTCACAAGGGCTTTTCCAGT+TGG - chr5_3:59612885-59612904 None:intergenic 50.0%
! GGAAGCACGTGTATATGCTG+AGG + chr5_3:59613546-59613565 Msa0821460:intron 50.0%
TCAGTCCAAGGAAGAGGAAG+AGG + chr5_3:59613758-59613777 Msa0821460:intron 50.0%
CTCTTCCTCTTCCTCTTCCT+TGG - chr5_3:59613766-59613785 None:intergenic 50.0%
CAACAAGAACCATCCCTGCT+GGG - chr5_3:59614578-59614597 None:intergenic 50.0%
TCAACAAGAACCATCCCTGC+TGG - chr5_3:59614579-59614598 None:intergenic 50.0%
! CTTGTTGATAGACCTGCCGA+AGG + chr5_3:59614591-59614610 Msa0821460:CDS 50.0%
! TGATGCAGCTCTGGTTCCTT+CGG - chr5_3:59614610-59614629 None:intergenic 50.0%
GCGAAGCTCCGACAAGTTTA+TGG + chr5_3:59614726-59614745 Msa0821460:three_prime_UTR 50.0%
!! CTCTAGGACTAGGTGTGTCG+CGG + chr5_3:59610454-59610473 Msa0821460:intron 55.0%
!! AGGTGTCGGACACCTGTCTT+AGG - chr5_3:59610571-59610590 None:intergenic 55.0%
ATTAGGGGGTTGTAGCGGTG+TGG + chr5_3:59610709-59610728 Msa0821460:intron 55.0%
CCGGTCGAACTCGATTACCA+TGG + chr5_3:59611041-59611060 Msa0821460:intron 55.0%
TCTTAACCCTCCGGTTCCCA+GGG - chr5_3:59611075-59611094 None:intergenic 55.0%
!! AAGCATGGTCTCTGGAGCCA+GGG - chr5_3:59611573-59611592 None:intergenic 55.0%
AGGGAATCCGAACATGGTCG+TGG + chr5_3:59612258-59612277 Msa0821460:intron 55.0%
!!! TTTTGCGCTGGTTGTGCCCT+TGG - chr5_3:59614555-59614574 None:intergenic 55.0%
AACCAGCGCAAAACCCAGCA+GGG + chr5_3:59614562-59614581 Msa0821460:CDS 55.0%
CGCGACACACCTAGTCCTAG+AGG - chr5_3:59610456-59610475 None:intergenic 60.0%
!! CGGTGTGGAGGTATCCGTGT+CGG + chr5_3:59610724-59610743 Msa0821460:intron 60.0%
TTACCATGGGGCCTTTCCCC+TGG + chr5_3:59611055-59611074 Msa0821460:intron 60.0%
TACCATGGGGCCTTTCCCCT+GGG + chr5_3:59611056-59611075 Msa0821460:intron 60.0%
CTTAACCCTCCGGTTCCCAG+GGG - chr5_3:59611074-59611093 None:intergenic 60.0%
GTCTTAACCCTCCGGTTCCC+AGG - chr5_3:59611076-59611095 None:intergenic 60.0%
GGAGCCAGGGCCCTAAAGAA+AGG - chr5_3:59611560-59611579 None:intergenic 60.0%
!! CAAGCATGGTCTCTGGAGCC+AGG - chr5_3:59611574-59611593 None:intergenic 60.0%
CTGGCTCCAGAGACCATGCT+TGG + chr5_3:59611572-59611591 Msa0821460:intron 60.0%
CAACCAGCGCAAAACCCAGC+AGG + chr5_3:59614561-59614580 Msa0821460:CDS 60.0%
! ATCCCTGCTGGGTTTTGCGC+TGG - chr5_3:59614567-59614586 None:intergenic 60.0%
AGCGCAAAACCCAGCAGGGA+TGG + chr5_3:59614566-59614585 Msa0821460:CDS 60.0%
AGGGGGTTGTAGCGGTGTGG+AGG + chr5_3:59610712-59610731 Msa0821460:intron 65.0%
TTCCCAGGGGAAAGGCCCCA+TGG - chr5_3:59611061-59611080 None:intergenic 65.0%
CCTTTCCCCTGGGAACCGGA+GGG + chr5_3:59611066-59611085 Msa0821460:intron 65.0%
! GCAGCTCTGGTTCCTTCGGC+AGG - chr5_3:59614606-59614625 None:intergenic 65.0%
GGGGCCTTTCCCCTGGGAAC+CGG + chr5_3:59611062-59611081 Msa0821460:intron 70.0%
GCCTTTCCCCTGGGAACCGG+AGG + chr5_3:59611065-59611084 Msa0821460:intron 70.0%
CCCTCCGGTTCCCAGGGGAA+AGG - chr5_3:59611069-59611088 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 59609588 59614935 59609588 ID=Msa0821460;Name=Msa0821460
chr5_3 mRNA 59609588 59614935 59609588 ID=Msa0821460-mRNA-1;Parent=Msa0821460;Name=Msa0821460-mRNA-1;_AED=0.10;_eAED=0.10;_QI=198|0.83|0.71|1|1|1|7|294|200
chr5_3 exon 59609588 59609824 59609588 ID=Msa0821460-mRNA-1:exon:18142;Parent=Msa0821460-mRNA-1
chr5_3 exon 59609923 59610000 59609923 ID=Msa0821460-mRNA-1:exon:18143;Parent=Msa0821460-mRNA-1
chr5_3 exon 59611296 59611463 59611296 ID=Msa0821460-mRNA-1:exon:18144;Parent=Msa0821460-mRNA-1
chr5_3 exon 59611676 59611724 59611676 ID=Msa0821460-mRNA-1:exon:18145;Parent=Msa0821460-mRNA-1
chr5_3 exon 59612260 59612333 59612260 ID=Msa0821460-mRNA-1:exon:18146;Parent=Msa0821460-mRNA-1
chr5_3 exon 59613547 59613640 59613547 ID=Msa0821460-mRNA-1:exon:18147;Parent=Msa0821460-mRNA-1
chr5_3 exon 59614541 59614935 59614541 ID=Msa0821460-mRNA-1:exon:18148;Parent=Msa0821460-mRNA-1
chr5_3 five_prime_UTR 59609588 59609785 59609588 ID=Msa0821460-mRNA-1:five_prime_utr;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59609786 59609824 59609786 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59609923 59610000 59609923 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59611296 59611463 59611296 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59611676 59611724 59611676 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59612260 59612333 59612260 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59613547 59613640 59613547 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 CDS 59614541 59614641 59614541 ID=Msa0821460-mRNA-1:cds;Parent=Msa0821460-mRNA-1
chr5_3 three_prime_UTR 59614642 59614935 59614642 ID=Msa0821460-mRNA-1:three_prime_utr;Parent=Msa0821460-mRNA-1
Gene Sequence

>Msa0821460

ATGGCTACGATCGGACACACCAACCTCAACGCCAAATTGGTTCTTCTTGGTGATATGGGTGCTGGAAAATCTAGCCTTGTTTTGCGTTTTGTTAAGGGTCAATTCCTTGAATTTCAGGAATCAACAATAGGGGCAGCATTTTTCTCCCAGACGTTGGCAGTAAATGACGCCACAGTGAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTAGCTCCCATGTACTATAGAGGCGCTGCTGCTGCTATCATTGTCTATGATATTACTAACTTGGAGTCATTTACACGAGCGAAGAAATGGGTCCAAGAGCTTCAGAAGCAAGGGAATCCGAACATGGTCGTGGCTTTTGCTGGTAACAAAGCTGACTTGGAAGATAAGAGGAAAGTGACAGCTGAAGAAGCACGTGTATATGCTGAGGAAAATGGTCTGTTTTTCATAGAGACGTCTGCGAAAACTGCAGCCAATGTTAATGACATATTTTATGAAATAGCCAAGAAGTTACCAAGGGCACAACCAGCGCAAAACCCAGCAGGGATGGTTCTTGTTGATAGACCTGCCGAAGGAACCAGAGCTGCATCATGTTGTTCATAA
Protein sequence

>Msa0821460

MATIGHTNLNAKLVLLGDMGAGKSSLVLRFVKGQFLEFQESTIGAAFFSQTLAVNDATVKFEIWDTAGQERYHSLAPMYYRGAAAAIIVYDITNLESFTRAKKWVQELQKQGNPNMVVAFAGNKADLEDKRKVTAEEARVYAEENGLFFIETSAKTAANVNDIFYEIAKKLPRAQPAQNPAGMVLVDRPAEGTRAASCCS*