AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0823050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823050 MtrunA17_Chr5g0408111 97.037 135 3 1 1 135 1 134 4.25e-90 257
Msa0823050 MtrunA17_Chr5g0393651 47.436 78 41 0 55 132 65 142 7.28e-24 89.7
Msa0823050 MtrunA17_Chr5g0402651 45.946 74 39 1 62 135 271 343 1.44e-18 79.7
Msa0823050 MtrunA17_Chr5g0402651 42.466 73 41 1 58 130 134 205 1.35e-17 77.0
Msa0823050 MtrunA17_Chr3g0100001 41.304 92 52 1 44 133 6 97 1.26e-17 72.8
Msa0823050 MtrunA17_Chr3g0100321 37.374 99 61 1 34 132 138 235 2.20e-17 76.6
Msa0823050 MtrunA17_Chr5g0408121 37.662 77 48 0 55 131 26 102 2.48e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823050 AT2G35605.1 55.303 132 35 3 4 135 2 109 1.30e-39 129
Msa0823050 AT1G31760.1 60.606 99 38 1 37 134 13 111 2.33e-35 118
Msa0823050 AT3G03590.1 58.621 87 35 1 49 135 58 143 7.82e-32 110
Msa0823050 AT2G14880.1 43.860 114 60 1 19 132 32 141 4.56e-30 105
Msa0823050 AT4G34290.1 47.674 86 45 0 47 132 59 144 1.07e-28 102
Msa0823050 AT3G48600.1 53.165 79 36 1 54 131 33 111 9.89e-23 88.6
Msa0823050 AT3G48600.2 53.165 79 36 1 54 131 33 111 2.74e-22 88.6
Msa0823050 AT4G26810.2 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0823050 AT4G26810.1 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0823050 AT4G22360.1 38.318 107 62 2 29 132 167 272 1.76e-17 77.4
Msa0823050 AT4G22360.1 36.047 86 52 2 52 135 301 385 3.29e-11 59.7
Msa0823050 AT1G49520.1 41.975 81 46 1 51 131 246 325 3.19e-15 71.2
Msa0823050 AT1G49520.1 38.272 81 49 1 51 131 97 176 2.22e-14 68.9
Msa0823050 AT3G19080.2 46.774 62 33 0 51 112 259 320 1.35e-13 66.6
Msa0823050 AT3G19080.2 38.750 80 48 1 51 130 111 189 3.06e-12 62.8
Msa0823050 AT3G19080.1 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823050 AT3G19080.1 40.260 77 43 2 60 134 385 460 1.10e-12 63.9
Msa0823050 AT3G19080.1 38.750 80 48 1 51 130 111 189 5.20e-12 62.0
Msa0823050 AT3G19080.4 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823050 AT3G19080.4 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0823050 AT3G19080.4 38.750 80 48 1 51 130 111 189 5.21e-12 62.0
Msa0823050 AT3G19080.3 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823050 AT3G19080.3 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0823050 AT3G19080.3 38.750 80 48 1 51 130 111 189 5.21e-12 62.0

Find 55 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCTCCTTCTTGTTATT+AGG 0.242982 5_3:-61663016 None:intergenic
CTGAAGCTGTTAAGAAAGTT+TGG 0.251130 5_3:+61661816 Msa0823050:CDS
TTTATCTTTCCCATCGAAAA+TGG 0.302387 5_3:-61663059 None:intergenic
TATGTGTCCTCTACTTCCTT+TGG 0.328846 5_3:+61663336 Msa0823050:three_prime_UTR
GGAAGTAGAGGACACATAAC+AGG 0.342700 5_3:-61663331 None:intergenic
TTGGTGCGCCTGAAGTTTCT+AGG 0.344233 5_3:+61661792 Msa0823050:CDS
TTCAGCTTGATTTCACGAAA+TGG 0.371326 5_3:-61663117 None:intergenic
TATCAAGCTGCAGAATCTTC+AGG 0.384496 5_3:+61661844 Msa0823050:CDS
AAACTTTAACTTGGGTTTGA+AGG 0.385086 5_3:+61663260 Msa0823050:three_prime_UTR
TGTAATATCAAACTTTAACT+TGG 0.404110 5_3:+61663251 Msa0823050:three_prime_UTR
TCTGAGCTCGGTAATTTCAT+TGG 0.417141 5_3:+61661773 Msa0823050:CDS
GAAATGGGTAGCTAGCAATT+TGG 0.423194 5_3:-61663101 None:intergenic
AAGAAGGCGGCTGCATCCGC+CGG 0.443418 5_3:+61661656 Msa0823050:CDS
TGAAGCTGTTAAGAAAGTTT+GGG 0.446267 5_3:+61661817 Msa0823050:CDS
CAGAATGCTAATGGCGGCGG+CGG 0.448004 5_3:+61661619 Msa0823050:CDS
ATTCGCCGGCAGAATGCTAA+TGG 0.448608 5_3:+61661610 Msa0823050:CDS
GACGGTTGTGGATGCAGCTC+CGG 0.455046 5_3:-61661675 None:intergenic
GCTTCAGTCCTAGAAACTTC+AGG 0.456584 5_3:-61661800 None:intergenic
AGGGATACAGAAGGTAGTTC+CGG 0.456709 5_3:+61661745 Msa0823050:CDS
CGCGATCATGGCACCAGCTA+GGG 0.457104 5_3:+61661577 Msa0823050:exon
GTAATATCAAACTTTAACTT+GGG 0.457408 5_3:+61663252 Msa0823050:three_prime_UTR
AAGCTGAAATTCAGCTCTGA+TGG 0.460067 5_3:+61663132 Msa0823050:exon
TCAGCTTGATTTCACGAAAT+GGG 0.467447 5_3:-61663116 None:intergenic
CTAATGGCGGCGGCGGCGAA+AGG 0.468371 5_3:+61661626 Msa0823050:CDS
AGCTAGCAATTTGGCAATCT+CGG 0.474611 5_3:-61663092 None:intergenic
GGCGAAAGGAGCCACGAAGA+AGG 0.475511 5_3:+61661640 Msa0823050:CDS
TTCGATGTTACTTCAACGGT+TGG 0.482707 5_3:-61663225 None:intergenic
CGGCAGAATGCTAATGGCGG+CGG 0.488744 5_3:+61661616 Msa0823050:CDS
GAAAACCGCCGTGAAGAAAA+CGG 0.489192 5_3:+61661703 Msa0823050:CDS
GGATGCAGCCGCCTTCTTCG+TGG 0.510322 5_3:-61661651 None:intergenic
CGCCGCCATTAGCATTCTGC+CGG 0.516612 5_3:-61661615 None:intergenic
AAATTTCGATGTTACTTCAA+CGG 0.519674 5_3:-61663229 None:intergenic
TCGCGATCATGGCACCAGCT+AGG 0.520628 5_3:+61661576 Msa0823050:exon
GCAGAATCCTAATAACAAGA+AGG 0.524697 5_3:+61663009 Msa0823050:intron
CATGCTTTAGTACATTAAGT+AGG 0.530371 5_3:+61663172 Msa0823050:three_prime_UTR
GTTCCGGTCACTTCTGAGCT+CGG 0.533521 5_3:+61661761 Msa0823050:CDS
TAAAGCATGTTACCAACGTT+AGG 0.541793 5_3:-61663158 None:intergenic
CAAAGATCCAAAGGAAGTAG+AGG 0.547155 5_3:-61663343 None:intergenic
AATGCTCCGAAAACCCTAGC+TGG 0.551067 5_3:-61661590 None:intergenic
TTTCGATGGGAAAGATAAAG+TGG 0.555020 5_3:+61663063 Msa0823050:CDS
AACTACCTTCTGTATCCCTC+CGG 0.556811 5_3:-61661741 None:intergenic
CTTTAACTTGGGTTTGAAGG+TGG 0.557831 5_3:+61663263 Msa0823050:three_prime_UTR
GAAAGGAGCCACGAAGAAGG+CGG 0.599028 5_3:+61661643 Msa0823050:CDS
CGCCGGCAGAATGCTAATGG+CGG 0.604430 5_3:+61661613 Msa0823050:CDS
GGTTGTGGATGCAGCTCCGG+CGG 0.606697 5_3:-61661672 None:intergenic
ACACAGAGTCAAAGATCCAA+AGG 0.614582 5_3:-61663352 None:intergenic
ACGGTGACGAGCAAGAGTAC+CGG 0.616079 5_3:+61661722 Msa0823050:CDS
GAACATTAGTATACGTGAGT+CGG 0.621180 5_3:+61663430 Msa0823050:three_prime_UTR
TTACCGAGCTCAGAAGTGAC+CGG 0.624475 5_3:-61661764 None:intergenic
CTCTGATGGATTCCTAACGT+TGG 0.627575 5_3:+61663146 Msa0823050:three_prime_UTR
GTGACGAGCAAGAGTACCGG+AGG 0.655721 5_3:+61661725 Msa0823050:CDS
GAAGTAGAGGACACATAACA+GGG 0.670201 5_3:-61663330 None:intergenic
ACAATGTGAAATCAAGACAG+TGG 0.670448 5_3:-61663298 None:intergenic
GAGTACCGGAGGGATACAGA+AGG 0.675343 5_3:+61661736 Msa0823050:CDS
TGACGAGCAAGAGTACCGGA+GGG 0.724885 5_3:+61661726 Msa0823050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTAATAT+CGG + chr5_3:61662357-61662376 Msa0823050:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG + chr5_3:61662434-61662453 Msa0823050:intron 10.0%
!!! TCATTTTCTTAAATTAATAT+CGG + chr5_3:61662357-61662376 Msa0823050:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG + chr5_3:61662434-61662453 Msa0823050:intron 10.0%
!!! ATGTAATAATAGTTTGAAAT+CGG + chr5_3:61662498-61662517 Msa0823050:intron 15.0%
!!! ATGTAATAATAGTTTGAAAT+CGG + chr5_3:61662498-61662517 Msa0823050:intron 15.0%
!! AAGCTAAGGATATTATTTAT+AGG - chr5_3:61662689-61662708 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG + chr5_3:61662747-61662766 Msa0823050:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG + chr5_3:61662845-61662864 Msa0823050:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG + chr5_3:61662946-61662965 Msa0823050:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG + chr5_3:61663251-61663270 Msa0823050:three_prime_UTR 20.0%
!!! GTAATATCAAACTTTAACTT+GGG + chr5_3:61663252-61663271 Msa0823050:three_prime_UTR 20.0%
!! ATCGTTTAAAAACATCAATT+TGG - chr5_3:61663389-61663408 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG + chr5_3:61663391-61663410 Msa0823050:three_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG + chr5_3:61663392-61663411 Msa0823050:three_prime_UTR 20.0%
!! AAGCTAAGGATATTATTTAT+AGG - chr5_3:61662689-61662708 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG + chr5_3:61662747-61662766 Msa0823050:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG + chr5_3:61662845-61662864 Msa0823050:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG + chr5_3:61662946-61662965 Msa0823050:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG + chr5_3:61663251-61663270 Msa0823050:three_prime_UTR 20.0%
!!! GTAATATCAAACTTTAACTT+GGG + chr5_3:61663252-61663271 Msa0823050:three_prime_UTR 20.0%
!! ATCGTTTAAAAACATCAATT+TGG - chr5_3:61663389-61663408 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG + chr5_3:61663391-61663410 Msa0823050:three_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG + chr5_3:61663392-61663411 Msa0823050:three_prime_UTR 20.0%
!! AAAACTTTGATCAGGTTTTA+TGG + chr5_3:61661965-61661984 Msa0823050:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG + chr5_3:61661990-61662009 Msa0823050:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG + chr5_3:61662632-61662651 Msa0823050:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG - chr5_3:61662819-61662838 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG - chr5_3:61662823-61662842 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG - chr5_3:61662944-61662963 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG - chr5_3:61663232-61663251 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG + chr5_3:61663393-61663412 Msa0823050:three_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG + chr5_3:61663394-61663413 Msa0823050:three_prime_UTR 25.0%
!! AAAACTTTGATCAGGTTTTA+TGG + chr5_3:61661965-61661984 Msa0823050:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG + chr5_3:61661990-61662009 Msa0823050:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG + chr5_3:61662632-61662651 Msa0823050:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG - chr5_3:61662819-61662838 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG - chr5_3:61662823-61662842 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG - chr5_3:61662944-61662963 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG - chr5_3:61663232-61663251 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG + chr5_3:61663393-61663412 Msa0823050:three_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG + chr5_3:61663394-61663413 Msa0823050:three_prime_UTR 25.0%
TGAAGCTGTTAAGAAAGTTT+GGG + chr5_3:61661817-61661836 Msa0823050:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG + chr5_3:61661957-61661976 Msa0823050:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG + chr5_3:61662015-61662034 Msa0823050:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG + chr5_3:61662026-61662045 Msa0823050:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG + chr5_3:61662027-61662046 Msa0823050:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG - chr5_3:61662132-61662151 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG - chr5_3:61662332-61662351 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG + chr5_3:61662541-61662560 Msa0823050:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG + chr5_3:61662652-61662671 Msa0823050:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG - chr5_3:61662703-61662722 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG + chr5_3:61662706-61662725 Msa0823050:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG + chr5_3:61662771-61662790 Msa0823050:intron 30.0%
AGACAAAAATACAATACAGG+AGG - chr5_3:61662941-61662960 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG + chr5_3:61662951-61662970 Msa0823050:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG - chr5_3:61663019-61663038 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG - chr5_3:61663062-61663081 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG + chr5_3:61663172-61663191 Msa0823050:three_prime_UTR 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG + chr5_3:61663260-61663279 Msa0823050:three_prime_UTR 30.0%
TGAAGCTGTTAAGAAAGTTT+GGG + chr5_3:61661817-61661836 Msa0823050:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG + chr5_3:61661957-61661976 Msa0823050:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG + chr5_3:61662015-61662034 Msa0823050:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG + chr5_3:61662026-61662045 Msa0823050:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG + chr5_3:61662027-61662046 Msa0823050:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG - chr5_3:61662132-61662151 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG - chr5_3:61662332-61662351 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG + chr5_3:61662541-61662560 Msa0823050:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG + chr5_3:61662652-61662671 Msa0823050:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG - chr5_3:61662703-61662722 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG + chr5_3:61662706-61662725 Msa0823050:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG + chr5_3:61662771-61662790 Msa0823050:intron 30.0%
AGACAAAAATACAATACAGG+AGG - chr5_3:61662941-61662960 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG + chr5_3:61662951-61662970 Msa0823050:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG - chr5_3:61663019-61663038 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG - chr5_3:61663062-61663081 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG + chr5_3:61663172-61663191 Msa0823050:three_prime_UTR 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG + chr5_3:61663260-61663279 Msa0823050:three_prime_UTR 30.0%
CTGAAGCTGTTAAGAAAGTT+TGG + chr5_3:61661816-61661835 Msa0823050:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG + chr5_3:61662080-61662099 Msa0823050:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG + chr5_3:61662393-61662412 Msa0823050:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG + chr5_3:61662544-61662563 Msa0823050:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG + chr5_3:61663009-61663028 Msa0823050:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG + chr5_3:61663049-61663068 Msa0823050:CDS 35.0%
! AACTGAAGACCATTTTCGAT+GGG + chr5_3:61663050-61663069 Msa0823050:CDS 35.0%
TTTCGATGGGAAAGATAAAG+TGG + chr5_3:61663063-61663082 Msa0823050:CDS 35.0%
TCAGCTTGATTTCACGAAAT+GGG - chr5_3:61663119-61663138 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG - chr5_3:61663120-61663139 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG - chr5_3:61663161-61663180 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG - chr5_3:61663301-61663320 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG + chr5_3:61663430-61663449 Msa0823050:three_prime_UTR 35.0%
CTGAAGCTGTTAAGAAAGTT+TGG + chr5_3:61661816-61661835 Msa0823050:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG + chr5_3:61662080-61662099 Msa0823050:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG + chr5_3:61662393-61662412 Msa0823050:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG + chr5_3:61662544-61662563 Msa0823050:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG + chr5_3:61663009-61663028 Msa0823050:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG + chr5_3:61663049-61663068 Msa0823050:CDS 35.0%
! AACTGAAGACCATTTTCGAT+GGG + chr5_3:61663050-61663069 Msa0823050:CDS 35.0%
TTTCGATGGGAAAGATAAAG+TGG + chr5_3:61663063-61663082 Msa0823050:CDS 35.0%
TCAGCTTGATTTCACGAAAT+GGG - chr5_3:61663119-61663138 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG - chr5_3:61663120-61663139 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG - chr5_3:61663161-61663180 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG - chr5_3:61663301-61663320 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG + chr5_3:61663430-61663449 Msa0823050:three_prime_UTR 35.0%
! CATTTTTCTCATCGCGATCA+TGG + chr5_3:61661565-61661584 Msa0823050:exon 40.0%
TCTGAGCTCGGTAATTTCAT+TGG + chr5_3:61661773-61661792 Msa0823050:CDS 40.0%
TATCAAGCTGCAGAATCTTC+AGG + chr5_3:61661844-61661863 Msa0823050:CDS 40.0%
!! AGAAGTGTCAAGTGTTATCG+AGG - chr5_3:61662260-61662279 None:intergenic 40.0%
CACTTGACACTTCTGATTGA+AGG + chr5_3:61662266-61662285 Msa0823050:intron 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG - chr5_3:61662321-61662340 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG + chr5_3:61662570-61662589 Msa0823050:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG - chr5_3:61662906-61662925 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG + chr5_3:61662966-61662985 Msa0823050:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG - chr5_3:61663095-61663114 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG - chr5_3:61663104-61663123 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG + chr5_3:61663132-61663151 Msa0823050:exon 40.0%
! TTCGATGTTACTTCAACGGT+TGG - chr5_3:61663228-61663247 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG + chr5_3:61663263-61663282 Msa0823050:three_prime_UTR 40.0%
GAAGTAGAGGACACATAACA+GGG - chr5_3:61663333-61663352 None:intergenic 40.0%
TATGTGTCCTCTACTTCCTT+TGG + chr5_3:61663336-61663355 Msa0823050:three_prime_UTR 40.0%
CAAAGATCCAAAGGAAGTAG+AGG - chr5_3:61663346-61663365 None:intergenic 40.0%
ACACAGAGTCAAAGATCCAA+AGG - chr5_3:61663355-61663374 None:intergenic 40.0%
! CATTTTTCTCATCGCGATCA+TGG + chr5_3:61661565-61661584 Msa0823050:exon 40.0%
TCTGAGCTCGGTAATTTCAT+TGG + chr5_3:61661773-61661792 Msa0823050:CDS 40.0%
TATCAAGCTGCAGAATCTTC+AGG + chr5_3:61661844-61661863 Msa0823050:CDS 40.0%
!! AGAAGTGTCAAGTGTTATCG+AGG - chr5_3:61662260-61662279 None:intergenic 40.0%
CACTTGACACTTCTGATTGA+AGG + chr5_3:61662266-61662285 Msa0823050:intron 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG - chr5_3:61662321-61662340 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG + chr5_3:61662570-61662589 Msa0823050:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG - chr5_3:61662906-61662925 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG + chr5_3:61662966-61662985 Msa0823050:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG - chr5_3:61663095-61663114 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG - chr5_3:61663104-61663123 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG + chr5_3:61663132-61663151 Msa0823050:exon 40.0%
! TTCGATGTTACTTCAACGGT+TGG - chr5_3:61663228-61663247 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG + chr5_3:61663263-61663282 Msa0823050:three_prime_UTR 40.0%
GAAGTAGAGGACACATAACA+GGG - chr5_3:61663333-61663352 None:intergenic 40.0%
TATGTGTCCTCTACTTCCTT+TGG + chr5_3:61663336-61663355 Msa0823050:three_prime_UTR 40.0%
CAAAGATCCAAAGGAAGTAG+AGG - chr5_3:61663346-61663365 None:intergenic 40.0%
ACACAGAGTCAAAGATCCAA+AGG - chr5_3:61663355-61663374 None:intergenic 40.0%
TCTCTCTCTCTCTCTCTCTA+GGG + chr5_3:61661516-61661535 Msa0823050:five_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG + chr5_3:61661703-61661722 Msa0823050:CDS 45.0%
AACTACCTTCTGTATCCCTC+CGG - chr5_3:61661744-61661763 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG + chr5_3:61661745-61661764 Msa0823050:CDS 45.0%
GCTTCAGTCCTAGAAACTTC+AGG - chr5_3:61661803-61661822 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG + chr5_3:61662197-61662216 Msa0823050:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG - chr5_3:61662526-61662545 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG - chr5_3:61662612-61662631 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG + chr5_3:61662887-61662906 Msa0823050:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG + chr5_3:61663146-61663165 Msa0823050:three_prime_UTR 45.0%
GGAAGTAGAGGACACATAAC+AGG - chr5_3:61663334-61663353 None:intergenic 45.0%
TCTCTCTCTCTCTCTCTCTA+GGG + chr5_3:61661516-61661535 Msa0823050:five_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG + chr5_3:61661703-61661722 Msa0823050:CDS 45.0%
AACTACCTTCTGTATCCCTC+CGG - chr5_3:61661744-61661763 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG + chr5_3:61661745-61661764 Msa0823050:CDS 45.0%
GCTTCAGTCCTAGAAACTTC+AGG - chr5_3:61661803-61661822 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG + chr5_3:61662197-61662216 Msa0823050:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG - chr5_3:61662526-61662545 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG - chr5_3:61662612-61662631 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG + chr5_3:61662887-61662906 Msa0823050:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG + chr5_3:61663146-61663165 Msa0823050:three_prime_UTR 45.0%
GGAAGTAGAGGACACATAAC+AGG - chr5_3:61663334-61663353 None:intergenic 45.0%
CTCTCTCTCTCTCTCTCTCT+AGG + chr5_3:61661507-61661526 Msa0823050:five_prime_UTR 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG + chr5_3:61661584-61661603 Msa0823050:CDS 50.0%
AATGCTCCGAAAACCCTAGC+TGG - chr5_3:61661593-61661612 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG + chr5_3:61661610-61661629 Msa0823050:CDS 50.0%
! GGTTTTCTTCACGACGGTTG+TGG - chr5_3:61661690-61661709 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG - chr5_3:61661714-61661733 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG - chr5_3:61661767-61661786 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG + chr5_3:61661792-61661811 Msa0823050:CDS 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG + chr5_3:61662282-61662301 Msa0823050:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG - chr5_3:61662304-61662323 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG + chr5_3:61662514-61662533 Msa0823050:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG - chr5_3:61662601-61662620 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG - chr5_3:61662602-61662621 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG - chr5_3:61662613-61662632 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG + chr5_3:61662901-61662920 Msa0823050:intron 50.0%
CTCTCTCTCTCTCTCTCTCT+AGG + chr5_3:61661507-61661526 Msa0823050:five_prime_UTR 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG + chr5_3:61661584-61661603 Msa0823050:CDS 50.0%
AATGCTCCGAAAACCCTAGC+TGG - chr5_3:61661593-61661612 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG + chr5_3:61661610-61661629 Msa0823050:CDS 50.0%
! GGTTTTCTTCACGACGGTTG+TGG - chr5_3:61661690-61661709 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG - chr5_3:61661714-61661733 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG - chr5_3:61661767-61661786 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG + chr5_3:61661792-61661811 Msa0823050:CDS 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG + chr5_3:61662282-61662301 Msa0823050:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG - chr5_3:61662304-61662323 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG + chr5_3:61662514-61662533 Msa0823050:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG - chr5_3:61662601-61662620 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG - chr5_3:61662602-61662621 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG - chr5_3:61662613-61662632 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG + chr5_3:61662901-61662920 Msa0823050:intron 50.0%
!! AGGGTTTTCGGAGCATTCGC+CGG + chr5_3:61661596-61661615 Msa0823050:CDS 55.0%
GAAAGGAGCCACGAAGAAGG+CGG + chr5_3:61661643-61661662 Msa0823050:CDS 55.0%
! CACGGCGGTTTTCTTCACGA+CGG - chr5_3:61661696-61661715 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG - chr5_3:61661711-61661730 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG + chr5_3:61661722-61661741 Msa0823050:CDS 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG + chr5_3:61661726-61661745 Msa0823050:CDS 55.0%
!! GAGTACCGGAGGGATACAGA+AGG + chr5_3:61661736-61661755 Msa0823050:CDS 55.0%
GTTCCGGTCACTTCTGAGCT+CGG + chr5_3:61661761-61661780 Msa0823050:CDS 55.0%
GTCAAACACATGCCGGACGA+TGG - chr5_3:61662297-61662316 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG + chr5_3:61662598-61662617 Msa0823050:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG + chr5_3:61662606-61662625 Msa0823050:intron 55.0%
TACGTGAGTCGGTGAGTGCA+AGG + chr5_3:61663441-61663460 Msa0823050:three_prime_UTR 55.0%
!! AGGGTTTTCGGAGCATTCGC+CGG + chr5_3:61661596-61661615 Msa0823050:CDS 55.0%
GAAAGGAGCCACGAAGAAGG+CGG + chr5_3:61661643-61661662 Msa0823050:CDS 55.0%
! CACGGCGGTTTTCTTCACGA+CGG - chr5_3:61661696-61661715 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG - chr5_3:61661711-61661730 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG + chr5_3:61661722-61661741 Msa0823050:CDS 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG + chr5_3:61661726-61661745 Msa0823050:CDS 55.0%
!! GAGTACCGGAGGGATACAGA+AGG + chr5_3:61661736-61661755 Msa0823050:CDS 55.0%
GTTCCGGTCACTTCTGAGCT+CGG + chr5_3:61661761-61661780 Msa0823050:CDS 55.0%
GTCAAACACATGCCGGACGA+TGG - chr5_3:61662297-61662316 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG + chr5_3:61662598-61662617 Msa0823050:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG + chr5_3:61662606-61662625 Msa0823050:intron 55.0%
TCGCGATCATGGCACCAGCT+AGG + chr5_3:61661576-61661595 Msa0823050:exon 60.0%
CGCGATCATGGCACCAGCTA+GGG + chr5_3:61661577-61661596 Msa0823050:exon 60.0%
CGCCGGCAGAATGCTAATGG+CGG + chr5_3:61661613-61661632 Msa0823050:CDS 60.0%
CGCCGCCATTAGCATTCTGC+CGG - chr5_3:61661618-61661637 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG + chr5_3:61661616-61661635 Msa0823050:CDS 60.0%
CAGAATGCTAATGGCGGCGG+CGG + chr5_3:61661619-61661638 Msa0823050:CDS 60.0%
GGCGAAAGGAGCCACGAAGA+AGG + chr5_3:61661640-61661659 Msa0823050:CDS 60.0%
GACGGTTGTGGATGCAGCTC+CGG - chr5_3:61661678-61661697 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG + chr5_3:61661725-61661744 Msa0823050:CDS 60.0%
TCGCGATCATGGCACCAGCT+AGG + chr5_3:61661576-61661595 Msa0823050:exon 60.0%
CGCGATCATGGCACCAGCTA+GGG + chr5_3:61661577-61661596 Msa0823050:exon 60.0%
CGCCGGCAGAATGCTAATGG+CGG + chr5_3:61661613-61661632 Msa0823050:CDS 60.0%
CGCCGCCATTAGCATTCTGC+CGG - chr5_3:61661618-61661637 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG + chr5_3:61661616-61661635 Msa0823050:CDS 60.0%
CAGAATGCTAATGGCGGCGG+CGG + chr5_3:61661619-61661638 Msa0823050:CDS 60.0%
GGCGAAAGGAGCCACGAAGA+AGG + chr5_3:61661640-61661659 Msa0823050:CDS 60.0%
GACGGTTGTGGATGCAGCTC+CGG - chr5_3:61661678-61661697 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG + chr5_3:61661725-61661744 Msa0823050:CDS 60.0%
GGATGCAGCCGCCTTCTTCG+TGG - chr5_3:61661654-61661673 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG + chr5_3:61661656-61661675 Msa0823050:CDS 65.0%
GGTTGTGGATGCAGCTCCGG+CGG - chr5_3:61661675-61661694 None:intergenic 65.0%
GGATGCAGCCGCCTTCTTCG+TGG - chr5_3:61661654-61661673 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG + chr5_3:61661656-61661675 Msa0823050:CDS 65.0%
GGTTGTGGATGCAGCTCCGG+CGG - chr5_3:61661675-61661694 None:intergenic 65.0%
CTAATGGCGGCGGCGGCGAA+AGG + chr5_3:61661626-61661645 Msa0823050:CDS 70.0%
CTAATGGCGGCGGCGGCGAA+AGG + chr5_3:61661626-61661645 Msa0823050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 61661464 61663462 61661464 ID=Msa0823050;Name=Msa0823050
chr5_3 mRNA 61661464 61663462 61661464 ID=Msa0823050-mRNA-1;Parent=Msa0823050;Name=Msa0823050-mRNA-1;_AED=0.04;_eAED=0.04;_QI=120|1|1|1|1|1|2|324|135
chr5_3 exon 61661464 61661865 61661464 ID=Msa0823050-mRNA-1:exon:19037;Parent=Msa0823050-mRNA-1
chr5_3 exon 61663013 61663462 61663013 ID=Msa0823050-mRNA-1:exon:19038;Parent=Msa0823050-mRNA-1
chr5_3 five_prime_UTR 61661464 61661583 61661464 ID=Msa0823050-mRNA-1:five_prime_utr;Parent=Msa0823050-mRNA-1
chr5_3 CDS 61661584 61661865 61661584 ID=Msa0823050-mRNA-1:cds;Parent=Msa0823050-mRNA-1
chr5_3 CDS 61663013 61663138 61663013 ID=Msa0823050-mRNA-1:cds;Parent=Msa0823050-mRNA-1
chr5_3 three_prime_UTR 61663139 61663462 61663139 ID=Msa0823050-mRNA-1:three_prime_utr;Parent=Msa0823050-mRNA-1
Gene Sequence

>Msa0823050

ATGGCACCAGCTAGGGTTTTCGGAGCATTCGCCGGCAGAATGCTAATGGCGGCGGCGGCGAAAGGAGCCACGAAGAAGGCGGCTGCATCCGCCGGAGCTGCATCCACAACCGTCGTGAAGAAAACCGCCGTGAAGAAAACGGTGACGAGCAAGAGTACCGGAGGGATACAGAAGGTAGTTCCGGTCACTTCTGAGCTCGGTAATTTCATTGGTGCGCCTGAAGTTTCTAGGACTGAAGCTGTTAAGAAAGTTTGGGAGTATATCAAGCTGCAGAATCTTCAGAATCCTAATAACAAGAAGGAGATCTTTTGTGATGACAAACTGAAGACCATTTTCGATGGGAAAGATAAAGTGGTCTTCACCGAGATTGCCAAATTGCTAGCTACCCATTTCGTGAAATCAAGCTGA
Protein sequence

>Msa0823050

MAPARVFGAFAGRMLMAAAAKGATKKAAASAGAASTTVVKKTAVKKTVTSKSTGGIQKVVPVTSELGNFIGAPEVSRTEAVKKVWEYIKLQNLQNPNNKKEIFCDDKLKTIFDGKDKVVFTEIAKLLATHFVKSS*