AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0718730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0718730 MtrunA17_Chr5g0408111 97.037 135 3 1 1 135 1 134 4.25e-90 257
Msa0718730 MtrunA17_Chr5g0393651 47.436 78 41 0 55 132 65 142 7.28e-24 89.7
Msa0718730 MtrunA17_Chr5g0402651 45.946 74 39 1 62 135 271 343 1.44e-18 79.7
Msa0718730 MtrunA17_Chr5g0402651 42.466 73 41 1 58 130 134 205 1.35e-17 77.0
Msa0718730 MtrunA17_Chr3g0100001 41.304 92 52 1 44 133 6 97 1.26e-17 72.8
Msa0718730 MtrunA17_Chr3g0100321 37.374 99 61 1 34 132 138 235 2.20e-17 76.6
Msa0718730 MtrunA17_Chr5g0408121 37.662 77 48 0 55 131 26 102 2.48e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0718730 AT2G35605.1 55.303 132 35 3 4 135 2 109 1.30e-39 129
Msa0718730 AT1G31760.1 60.606 99 38 1 37 134 13 111 2.33e-35 118
Msa0718730 AT3G03590.1 58.621 87 35 1 49 135 58 143 7.82e-32 110
Msa0718730 AT2G14880.1 43.860 114 60 1 19 132 32 141 4.56e-30 105
Msa0718730 AT4G34290.1 47.674 86 45 0 47 132 59 144 1.07e-28 102
Msa0718730 AT3G48600.1 53.165 79 36 1 54 131 33 111 9.89e-23 88.6
Msa0718730 AT3G48600.2 53.165 79 36 1 54 131 33 111 2.74e-22 88.6
Msa0718730 AT4G26810.2 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0718730 AT4G26810.1 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0718730 AT4G22360.1 38.318 107 62 2 29 132 167 272 1.76e-17 77.4
Msa0718730 AT4G22360.1 36.047 86 52 2 52 135 301 385 3.29e-11 59.7
Msa0718730 AT1G49520.1 41.975 81 46 1 51 131 246 325 3.19e-15 71.2
Msa0718730 AT1G49520.1 38.272 81 49 1 51 131 97 176 2.22e-14 68.9
Msa0718730 AT3G19080.2 46.774 62 33 0 51 112 259 320 1.35e-13 66.6
Msa0718730 AT3G19080.2 38.750 80 48 1 51 130 111 189 3.06e-12 62.8
Msa0718730 AT3G19080.1 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0718730 AT3G19080.1 40.260 77 43 2 60 134 385 460 1.10e-12 63.9
Msa0718730 AT3G19080.1 38.750 80 48 1 51 130 111 189 5.20e-12 62.0
Msa0718730 AT3G19080.4 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0718730 AT3G19080.4 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0718730 AT3G19080.4 38.750 80 48 1 51 130 111 189 5.21e-12 62.0
Msa0718730 AT3G19080.3 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0718730 AT3G19080.3 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0718730 AT3G19080.3 38.750 80 48 1 51 130 111 189 5.21e-12 62.0

Find 54 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCTCCTTCTTGTTATT+AGG 0.242982 5_1:+13028675 None:intergenic
CTGAAGCTGTTAAGAAAGTT+TGG 0.251130 5_1:-13029874 Msa0718730:CDS
TTTATCTTTCCCATCGAAAA+TGG 0.302387 5_1:+13028632 None:intergenic
GGAAGTAGAGGACACATAAC+AGG 0.342700 5_1:+13028360 None:intergenic
TTGGTGCGCCTGAAGTTTCT+AGG 0.344233 5_1:-13029898 Msa0718730:CDS
TTCAGCTTGATTTCACGAAA+TGG 0.371326 5_1:+13028574 None:intergenic
TATCAAGCTGCAGAATCTTC+AGG 0.384496 5_1:-13029846 Msa0718730:intron
AAACTTTAACTTGGGTTTGA+AGG 0.385086 5_1:-13028431 Msa0718730:three_prime_UTR
TGTAATATCAAACTTTAACT+TGG 0.404110 5_1:-13028440 Msa0718730:three_prime_UTR
TCTGAGCTCGGTAATTTCAT+TGG 0.417141 5_1:-13029917 Msa0718730:CDS
GAAATGGGTAGCTAGCAATT+TGG 0.423194 5_1:+13028590 None:intergenic
AAGAAGGCGGCTGCATCCGC+CGG 0.443418 5_1:-13030034 Msa0718730:CDS
TGAAGCTGTTAAGAAAGTTT+GGG 0.446267 5_1:-13029873 Msa0718730:CDS
CAGAATGCTAATGGCGGCGG+CGG 0.448004 5_1:-13030071 Msa0718730:CDS
ATTCGCCGGCAGAATGCTAA+TGG 0.448608 5_1:-13030080 Msa0718730:CDS
GACGGTTGTGGATGCAGCTC+CGG 0.455046 5_1:+13030015 None:intergenic
GCTTCAGTCCTAGAAACTTC+AGG 0.456584 5_1:+13029890 None:intergenic
AGGGATACAGAAGGTAGTTC+CGG 0.456709 5_1:-13029945 Msa0718730:CDS
CGCGATCATGGCACCAGCTA+GGG 0.457104 5_1:-13030113 Msa0718730:exon
GTAATATCAAACTTTAACTT+GGG 0.457408 5_1:-13028439 Msa0718730:three_prime_UTR
AAGCTGAAATTCAGCTCTGA+TGG 0.460067 5_1:-13028559 Msa0718730:exon
TCAGCTTGATTTCACGAAAT+GGG 0.467447 5_1:+13028575 None:intergenic
CTAATGGCGGCGGCGGCGAA+AGG 0.468371 5_1:-13030064 Msa0718730:CDS
AGCTAGCAATTTGGCAATCT+CGG 0.474611 5_1:+13028599 None:intergenic
GGCGAAAGGAGCCACGAAGA+AGG 0.475511 5_1:-13030050 Msa0718730:CDS
TTCGATGTTACTTCAACGGT+TGG 0.482707 5_1:+13028466 None:intergenic
CGGCAGAATGCTAATGGCGG+CGG 0.488744 5_1:-13030074 Msa0718730:CDS
GAAAACCGCCGTGAAGAAAA+CGG 0.489192 5_1:-13029987 Msa0718730:CDS
GGATGCAGCCGCCTTCTTCG+TGG 0.510322 5_1:+13030039 None:intergenic
CGCCGCCATTAGCATTCTGC+CGG 0.516612 5_1:+13030075 None:intergenic
AAATTTCGATGTTACTTCAA+CGG 0.519674 5_1:+13028462 None:intergenic
TCGCGATCATGGCACCAGCT+AGG 0.520628 5_1:-13030114 Msa0718730:exon
GCAGAATCCTAATAACAAGA+AGG 0.524697 5_1:-13028682 Msa0718730:intron
CATGCTTTAGTACATTAAGT+AGG 0.530371 5_1:-13028519 Msa0718730:three_prime_UTR
GTTCCGGTCACTTCTGAGCT+CGG 0.533521 5_1:-13029929 Msa0718730:CDS
CAAAGATTCAAAGGAAGTAG+AGG 0.538400 5_1:+13028348 None:intergenic
TAAAGCATGTTACCAACGTT+AGG 0.541793 5_1:+13028533 None:intergenic
ACACAGAGTCAAAGATTCAA+AGG 0.544655 5_1:+13028339 None:intergenic
AATGCTCCGAAAACCCTAGC+TGG 0.551067 5_1:+13030100 None:intergenic
TTTCGATGGGAAAGATAAAG+TGG 0.555020 5_1:-13028628 Msa0718730:CDS
AACTACCTTCTGTATCCCTC+CGG 0.556811 5_1:+13029949 None:intergenic
CTTTAACTTGGGTTTGAAGG+TGG 0.557831 5_1:-13028428 Msa0718730:three_prime_UTR
GAAAGGAGCCACGAAGAAGG+CGG 0.599028 5_1:-13030047 Msa0718730:CDS
CGCCGGCAGAATGCTAATGG+CGG 0.604430 5_1:-13030077 Msa0718730:CDS
GGTTGTGGATGCAGCTCCGG+CGG 0.606697 5_1:+13030018 None:intergenic
ACGGTGACGAGCAAGAGTAC+CGG 0.616079 5_1:-13029968 Msa0718730:CDS
GAACATTAGTATACGTGAGT+CGG 0.621180 5_1:-13028261 Msa0718730:three_prime_UTR
TTACCGAGCTCAGAAGTGAC+CGG 0.624475 5_1:+13029926 None:intergenic
CTCTGATGGATTCCTAACGT+TGG 0.627575 5_1:-13028545 Msa0718730:three_prime_UTR
GTGACGAGCAAGAGTACCGG+AGG 0.655721 5_1:-13029965 Msa0718730:CDS
GAAGTAGAGGACACATAACA+GGG 0.670201 5_1:+13028361 None:intergenic
ACAATGTGAAATCAAGACAG+TGG 0.670448 5_1:+13028393 None:intergenic
GAGTACCGGAGGGATACAGA+AGG 0.675343 5_1:-13029954 Msa0718730:CDS
TGACGAGCAAGAGTACCGGA+GGG 0.724885 5_1:-13029964 Msa0718730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTAATAT+CGG - chr5_1:13029133-13029152 Msa0718730:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG - chr5_1:13029210-13029229 Msa0718730:intron 10.0%
!!! TCATTTTCTTAAATTAATAT+CGG - chr5_1:13029133-13029152 Msa0718730:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG - chr5_1:13029210-13029229 Msa0718730:intron 10.0%
!!! ATGTAATAATAGTTTGAAAT+CGG - chr5_1:13029274-13029293 Msa0718730:intron 15.0%
!!! ATGTAATAATAGTTTGAAAT+CGG - chr5_1:13029274-13029293 Msa0718730:intron 15.0%
!! AAGCTAAGGATATTATTTAT+AGG + chr5_1:13029465-13029484 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG - chr5_1:13029523-13029542 Msa0718730:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG - chr5_1:13029621-13029640 Msa0718730:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG - chr5_1:13029722-13029741 Msa0718730:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG - chr5_1:13030027-13030046 Msa0718730:CDS 20.0%
!!! GTAATATCAAACTTTAACTT+GGG - chr5_1:13030028-13030047 Msa0718730:CDS 20.0%
!! ATCGTTTAAAAACATCAATT+TGG + chr5_1:13030165-13030184 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG - chr5_1:13030167-13030186 Msa0718730:five_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG - chr5_1:13030168-13030187 Msa0718730:five_prime_UTR 20.0%
!! AAGCTAAGGATATTATTTAT+AGG + chr5_1:13029465-13029484 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG - chr5_1:13029523-13029542 Msa0718730:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG - chr5_1:13029621-13029640 Msa0718730:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG - chr5_1:13029722-13029741 Msa0718730:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG - chr5_1:13030027-13030046 Msa0718730:CDS 20.0%
!!! GTAATATCAAACTTTAACTT+GGG - chr5_1:13030028-13030047 Msa0718730:CDS 20.0%
!! ATCGTTTAAAAACATCAATT+TGG + chr5_1:13030165-13030184 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG - chr5_1:13030167-13030186 Msa0718730:five_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG - chr5_1:13030168-13030187 Msa0718730:five_prime_UTR 20.0%
!! AAAACTTTGATCAGGTTTTA+TGG - chr5_1:13028742-13028761 Msa0718730:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG - chr5_1:13028767-13028786 Msa0718730:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG - chr5_1:13029408-13029427 Msa0718730:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG + chr5_1:13029595-13029614 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG + chr5_1:13029599-13029618 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG + chr5_1:13029720-13029739 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG + chr5_1:13030008-13030027 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG - chr5_1:13030169-13030188 Msa0718730:five_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG - chr5_1:13030170-13030189 Msa0718730:five_prime_UTR 25.0%
!! AAAACTTTGATCAGGTTTTA+TGG - chr5_1:13028742-13028761 Msa0718730:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG - chr5_1:13028767-13028786 Msa0718730:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG - chr5_1:13029408-13029427 Msa0718730:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG + chr5_1:13029595-13029614 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG + chr5_1:13029599-13029618 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG + chr5_1:13029720-13029739 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG + chr5_1:13030008-13030027 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG - chr5_1:13030169-13030188 Msa0718730:five_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG - chr5_1:13030170-13030189 Msa0718730:five_prime_UTR 25.0%
TGAAGCTGTTAAGAAAGTTT+GGG - chr5_1:13028594-13028613 Msa0718730:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG - chr5_1:13028734-13028753 Msa0718730:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG - chr5_1:13028792-13028811 Msa0718730:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG - chr5_1:13028803-13028822 Msa0718730:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG - chr5_1:13028804-13028823 Msa0718730:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG + chr5_1:13028909-13028928 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG + chr5_1:13029108-13029127 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG - chr5_1:13029317-13029336 Msa0718730:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG - chr5_1:13029428-13029447 Msa0718730:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG + chr5_1:13029479-13029498 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG - chr5_1:13029482-13029501 Msa0718730:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG - chr5_1:13029547-13029566 Msa0718730:intron 30.0%
AGACAAAAATACAATACAGG+AGG + chr5_1:13029717-13029736 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG - chr5_1:13029727-13029746 Msa0718730:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG + chr5_1:13029795-13029814 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG + chr5_1:13029838-13029857 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG - chr5_1:13029948-13029967 Msa0718730:CDS 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG - chr5_1:13030036-13030055 Msa0718730:CDS 30.0%
TGAAGCTGTTAAGAAAGTTT+GGG - chr5_1:13028594-13028613 Msa0718730:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG - chr5_1:13028734-13028753 Msa0718730:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG - chr5_1:13028792-13028811 Msa0718730:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG - chr5_1:13028803-13028822 Msa0718730:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG - chr5_1:13028804-13028823 Msa0718730:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG + chr5_1:13028909-13028928 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG + chr5_1:13029108-13029127 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG - chr5_1:13029317-13029336 Msa0718730:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG - chr5_1:13029428-13029447 Msa0718730:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG + chr5_1:13029479-13029498 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG - chr5_1:13029482-13029501 Msa0718730:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG - chr5_1:13029547-13029566 Msa0718730:intron 30.0%
AGACAAAAATACAATACAGG+AGG + chr5_1:13029717-13029736 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG - chr5_1:13029727-13029746 Msa0718730:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG + chr5_1:13029795-13029814 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG + chr5_1:13029838-13029857 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG - chr5_1:13029948-13029967 Msa0718730:CDS 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG - chr5_1:13030036-13030055 Msa0718730:CDS 30.0%
CTGAAGCTGTTAAGAAAGTT+TGG - chr5_1:13028593-13028612 Msa0718730:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG - chr5_1:13028857-13028876 Msa0718730:intron 35.0%
ACACTTACACTTCTGATTGA+AGG - chr5_1:13029042-13029061 Msa0718730:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG - chr5_1:13029169-13029188 Msa0718730:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG - chr5_1:13029320-13029339 Msa0718730:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG - chr5_1:13029785-13029804 Msa0718730:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG - chr5_1:13029825-13029844 Msa0718730:intron 35.0%
! AACTGAAGACCATTTTCGAT+GGG - chr5_1:13029826-13029845 Msa0718730:intron 35.0%
TTTCGATGGGAAAGATAAAG+TGG - chr5_1:13029839-13029858 Msa0718730:intron 35.0%
TCAGCTTGATTTCACGAAAT+GGG + chr5_1:13029895-13029914 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG + chr5_1:13029896-13029915 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG + chr5_1:13029937-13029956 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG + chr5_1:13030077-13030096 None:intergenic 35.0%
CAAAGATTCAAAGGAAGTAG+AGG + chr5_1:13030122-13030141 None:intergenic 35.0%
ACACAGAGTCAAAGATTCAA+AGG + chr5_1:13030131-13030150 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG - chr5_1:13030206-13030225 Msa0718730:five_prime_UTR 35.0%
CTGAAGCTGTTAAGAAAGTT+TGG - chr5_1:13028593-13028612 Msa0718730:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG - chr5_1:13028857-13028876 Msa0718730:intron 35.0%
ACACTTACACTTCTGATTGA+AGG - chr5_1:13029042-13029061 Msa0718730:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG - chr5_1:13029169-13029188 Msa0718730:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG - chr5_1:13029320-13029339 Msa0718730:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG - chr5_1:13029785-13029804 Msa0718730:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG - chr5_1:13029825-13029844 Msa0718730:intron 35.0%
! AACTGAAGACCATTTTCGAT+GGG - chr5_1:13029826-13029845 Msa0718730:intron 35.0%
TTTCGATGGGAAAGATAAAG+TGG - chr5_1:13029839-13029858 Msa0718730:intron 35.0%
TCAGCTTGATTTCACGAAAT+GGG + chr5_1:13029895-13029914 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG + chr5_1:13029896-13029915 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG + chr5_1:13029937-13029956 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG + chr5_1:13030077-13030096 None:intergenic 35.0%
CAAAGATTCAAAGGAAGTAG+AGG + chr5_1:13030122-13030141 None:intergenic 35.0%
ACACAGAGTCAAAGATTCAA+AGG + chr5_1:13030131-13030150 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG - chr5_1:13030206-13030225 Msa0718730:five_prime_UTR 35.0%
! CATTTTTCTCATCGCGATCA+TGG - chr5_1:13028342-13028361 Msa0718730:three_prime_UTR 40.0%
TCTGAGCTCGGTAATTTCAT+TGG - chr5_1:13028550-13028569 Msa0718730:three_prime_UTR 40.0%
TATCAAGCTGCAGAATCTTC+AGG - chr5_1:13028621-13028640 Msa0718730:CDS 40.0%
! CAGAAGTGTAAGTGTTATCG+AGG + chr5_1:13029037-13029056 None:intergenic 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG + chr5_1:13029097-13029116 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG - chr5_1:13029346-13029365 Msa0718730:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG + chr5_1:13029682-13029701 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG - chr5_1:13029742-13029761 Msa0718730:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG + chr5_1:13029871-13029890 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG + chr5_1:13029880-13029899 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG - chr5_1:13029908-13029927 Msa0718730:CDS 40.0%
! TTCGATGTTACTTCAACGGT+TGG + chr5_1:13030004-13030023 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG - chr5_1:13030039-13030058 Msa0718730:CDS 40.0%
GAAGTAGAGGACACATAACA+GGG + chr5_1:13030109-13030128 None:intergenic 40.0%
! CATTTTTCTCATCGCGATCA+TGG - chr5_1:13028342-13028361 Msa0718730:three_prime_UTR 40.0%
TCTGAGCTCGGTAATTTCAT+TGG - chr5_1:13028550-13028569 Msa0718730:three_prime_UTR 40.0%
TATCAAGCTGCAGAATCTTC+AGG - chr5_1:13028621-13028640 Msa0718730:CDS 40.0%
! CAGAAGTGTAAGTGTTATCG+AGG + chr5_1:13029037-13029056 None:intergenic 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG + chr5_1:13029097-13029116 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG - chr5_1:13029346-13029365 Msa0718730:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG + chr5_1:13029682-13029701 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG - chr5_1:13029742-13029761 Msa0718730:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG + chr5_1:13029871-13029890 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG + chr5_1:13029880-13029899 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG - chr5_1:13029908-13029927 Msa0718730:CDS 40.0%
! TTCGATGTTACTTCAACGGT+TGG + chr5_1:13030004-13030023 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG - chr5_1:13030039-13030058 Msa0718730:CDS 40.0%
GAAGTAGAGGACACATAACA+GGG + chr5_1:13030109-13030128 None:intergenic 40.0%
ACTCTCTCTCTCTCTCTCTA+GGG - chr5_1:13028293-13028312 Msa0718730:three_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG - chr5_1:13028480-13028499 Msa0718730:three_prime_UTR 45.0%
AACTACCTTCTGTATCCCTC+CGG + chr5_1:13028521-13028540 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG - chr5_1:13028522-13028541 Msa0718730:three_prime_UTR 45.0%
GCTTCAGTCCTAGAAACTTC+AGG + chr5_1:13028580-13028599 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG - chr5_1:13028974-13028993 Msa0718730:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG + chr5_1:13029302-13029321 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG + chr5_1:13029388-13029407 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG - chr5_1:13029663-13029682 Msa0718730:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG - chr5_1:13029922-13029941 Msa0718730:CDS 45.0%
GGAAGTAGAGGACACATAAC+AGG + chr5_1:13030110-13030129 None:intergenic 45.0%
ACTCTCTCTCTCTCTCTCTA+GGG - chr5_1:13028293-13028312 Msa0718730:three_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG - chr5_1:13028480-13028499 Msa0718730:three_prime_UTR 45.0%
AACTACCTTCTGTATCCCTC+CGG + chr5_1:13028521-13028540 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG - chr5_1:13028522-13028541 Msa0718730:three_prime_UTR 45.0%
GCTTCAGTCCTAGAAACTTC+AGG + chr5_1:13028580-13028599 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG - chr5_1:13028974-13028993 Msa0718730:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG + chr5_1:13029302-13029321 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG + chr5_1:13029388-13029407 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG - chr5_1:13029663-13029682 Msa0718730:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG - chr5_1:13029922-13029941 Msa0718730:CDS 45.0%
GGAAGTAGAGGACACATAAC+AGG + chr5_1:13030110-13030129 None:intergenic 45.0%
CACTCTCTCTCTCTCTCTCT+AGG - chr5_1:13028292-13028311 Msa0718730:three_prime_UTR 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG - chr5_1:13028361-13028380 Msa0718730:three_prime_UTR 50.0%
AATGCTCCGAAAACCCTAGC+TGG + chr5_1:13028370-13028389 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG - chr5_1:13028387-13028406 Msa0718730:three_prime_UTR 50.0%
! GGTTTTCTTCACGACGGTTG+TGG + chr5_1:13028467-13028486 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG + chr5_1:13028491-13028510 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG + chr5_1:13028544-13028563 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG - chr5_1:13028569-13028588 Msa0718730:exon 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG - chr5_1:13029058-13029077 Msa0718730:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG + chr5_1:13029080-13029099 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG - chr5_1:13029290-13029309 Msa0718730:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG + chr5_1:13029377-13029396 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG + chr5_1:13029378-13029397 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG + chr5_1:13029389-13029408 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG - chr5_1:13029677-13029696 Msa0718730:intron 50.0%
CACTCTCTCTCTCTCTCTCT+AGG - chr5_1:13028292-13028311 Msa0718730:three_prime_UTR 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG - chr5_1:13028361-13028380 Msa0718730:three_prime_UTR 50.0%
AATGCTCCGAAAACCCTAGC+TGG + chr5_1:13028370-13028389 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG - chr5_1:13028387-13028406 Msa0718730:three_prime_UTR 50.0%
! GGTTTTCTTCACGACGGTTG+TGG + chr5_1:13028467-13028486 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG + chr5_1:13028491-13028510 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG + chr5_1:13028544-13028563 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG - chr5_1:13028569-13028588 Msa0718730:exon 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG - chr5_1:13029058-13029077 Msa0718730:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG + chr5_1:13029080-13029099 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG - chr5_1:13029290-13029309 Msa0718730:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG + chr5_1:13029377-13029396 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG + chr5_1:13029378-13029397 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG + chr5_1:13029389-13029408 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG - chr5_1:13029677-13029696 Msa0718730:intron 50.0%
!! AGGGTTTTCGGAGCATTCGC+CGG - chr5_1:13028373-13028392 Msa0718730:three_prime_UTR 55.0%
GAAAGGAGCCACGAAGAAGG+CGG - chr5_1:13028420-13028439 Msa0718730:three_prime_UTR 55.0%
! CACGGCGGTTTTCTTCACGA+CGG + chr5_1:13028473-13028492 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG + chr5_1:13028488-13028507 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG - chr5_1:13028499-13028518 Msa0718730:three_prime_UTR 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG - chr5_1:13028503-13028522 Msa0718730:three_prime_UTR 55.0%
!! GAGTACCGGAGGGATACAGA+AGG - chr5_1:13028513-13028532 Msa0718730:three_prime_UTR 55.0%
GTTCCGGTCACTTCTGAGCT+CGG - chr5_1:13028538-13028557 Msa0718730:three_prime_UTR 55.0%
GTCAAACACATGCCGGACGA+TGG + chr5_1:13029073-13029092 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG - chr5_1:13029374-13029393 Msa0718730:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG - chr5_1:13029382-13029401 Msa0718730:intron 55.0%
TACGTGAGTCGGTGAGTGCA+AGG - chr5_1:13030217-13030236 Msa0718730:five_prime_UTR 55.0%
!! AGGGTTTTCGGAGCATTCGC+CGG - chr5_1:13028373-13028392 Msa0718730:three_prime_UTR 55.0%
GAAAGGAGCCACGAAGAAGG+CGG - chr5_1:13028420-13028439 Msa0718730:three_prime_UTR 55.0%
! CACGGCGGTTTTCTTCACGA+CGG + chr5_1:13028473-13028492 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG + chr5_1:13028488-13028507 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG - chr5_1:13028499-13028518 Msa0718730:three_prime_UTR 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG - chr5_1:13028503-13028522 Msa0718730:three_prime_UTR 55.0%
!! GAGTACCGGAGGGATACAGA+AGG - chr5_1:13028513-13028532 Msa0718730:three_prime_UTR 55.0%
GTTCCGGTCACTTCTGAGCT+CGG - chr5_1:13028538-13028557 Msa0718730:three_prime_UTR 55.0%
GTCAAACACATGCCGGACGA+TGG + chr5_1:13029073-13029092 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG - chr5_1:13029374-13029393 Msa0718730:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG - chr5_1:13029382-13029401 Msa0718730:intron 55.0%
TCGCGATCATGGCACCAGCT+AGG - chr5_1:13028353-13028372 Msa0718730:three_prime_UTR 60.0%
CGCGATCATGGCACCAGCTA+GGG - chr5_1:13028354-13028373 Msa0718730:three_prime_UTR 60.0%
CGCCGGCAGAATGCTAATGG+CGG - chr5_1:13028390-13028409 Msa0718730:three_prime_UTR 60.0%
CGCCGCCATTAGCATTCTGC+CGG + chr5_1:13028395-13028414 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG - chr5_1:13028393-13028412 Msa0718730:three_prime_UTR 60.0%
CAGAATGCTAATGGCGGCGG+CGG - chr5_1:13028396-13028415 Msa0718730:three_prime_UTR 60.0%
GGCGAAAGGAGCCACGAAGA+AGG - chr5_1:13028417-13028436 Msa0718730:three_prime_UTR 60.0%
GACGGTTGTGGATGCAGCTC+CGG + chr5_1:13028455-13028474 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG - chr5_1:13028502-13028521 Msa0718730:three_prime_UTR 60.0%
TCGCGATCATGGCACCAGCT+AGG - chr5_1:13028353-13028372 Msa0718730:three_prime_UTR 60.0%
CGCGATCATGGCACCAGCTA+GGG - chr5_1:13028354-13028373 Msa0718730:three_prime_UTR 60.0%
CGCCGGCAGAATGCTAATGG+CGG - chr5_1:13028390-13028409 Msa0718730:three_prime_UTR 60.0%
CGCCGCCATTAGCATTCTGC+CGG + chr5_1:13028395-13028414 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG - chr5_1:13028393-13028412 Msa0718730:three_prime_UTR 60.0%
CAGAATGCTAATGGCGGCGG+CGG - chr5_1:13028396-13028415 Msa0718730:three_prime_UTR 60.0%
GGCGAAAGGAGCCACGAAGA+AGG - chr5_1:13028417-13028436 Msa0718730:three_prime_UTR 60.0%
GACGGTTGTGGATGCAGCTC+CGG + chr5_1:13028455-13028474 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG - chr5_1:13028502-13028521 Msa0718730:three_prime_UTR 60.0%
GGATGCAGCCGCCTTCTTCG+TGG + chr5_1:13028431-13028450 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG - chr5_1:13028433-13028452 Msa0718730:three_prime_UTR 65.0%
GGTTGTGGATGCAGCTCCGG+CGG + chr5_1:13028452-13028471 None:intergenic 65.0%
GGATGCAGCCGCCTTCTTCG+TGG + chr5_1:13028431-13028450 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG - chr5_1:13028433-13028452 Msa0718730:three_prime_UTR 65.0%
GGTTGTGGATGCAGCTCCGG+CGG + chr5_1:13028452-13028471 None:intergenic 65.0%
CTAATGGCGGCGGCGGCGAA+AGG - chr5_1:13028403-13028422 Msa0718730:three_prime_UTR 70.0%
CTAATGGCGGCGGCGGCGAA+AGG - chr5_1:13028403-13028422 Msa0718730:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 13028251 13030238 13028251 ID=Msa0718730;Name=Msa0718730
chr5_1 mRNA 13028251 13030238 13028251 ID=Msa0718730-mRNA-1;Parent=Msa0718730;Name=Msa0718730-mRNA-1;_AED=0.04;_eAED=0.04;_QI=110|1|1|1|1|1|2|324|135
chr5_1 exon 13028251 13028700 13028251 ID=Msa0718730-mRNA-1:exon:5359;Parent=Msa0718730-mRNA-1
chr5_1 exon 13029847 13030238 13029847 ID=Msa0718730-mRNA-1:exon:5358;Parent=Msa0718730-mRNA-1
chr5_1 five_prime_UTR 13030129 13030238 13030129 ID=Msa0718730-mRNA-1:five_prime_utr;Parent=Msa0718730-mRNA-1
chr5_1 CDS 13029847 13030128 13029847 ID=Msa0718730-mRNA-1:cds;Parent=Msa0718730-mRNA-1
chr5_1 CDS 13028575 13028700 13028575 ID=Msa0718730-mRNA-1:cds;Parent=Msa0718730-mRNA-1
chr5_1 three_prime_UTR 13028251 13028574 13028251 ID=Msa0718730-mRNA-1:three_prime_utr;Parent=Msa0718730-mRNA-1
Gene Sequence

>Msa0718730

ATGGCACCAGCTAGGGTTTTCGGAGCATTCGCCGGCAGAATGCTAATGGCGGCGGCGGCGAAAGGAGCCACGAAGAAGGCGGCTGCATCCGCCGGAGCTGCATCCACAACCGTCGTGAAGAAAACCGCCGTGAAGAAAACGGTGACGAGCAAGAGTACCGGAGGGATACAGAAGGTAGTTCCGGTCACTTCTGAGCTCGGTAATTTCATTGGTGCGCCTGAAGTTTCTAGGACTGAAGCTGTTAAGAAAGTTTGGGAGTATATCAAGCTGCAGAATCTTCAGAATCCTAATAACAAGAAGGAGATCTTTTGTGATGACAAACTGAAGACCATTTTCGATGGGAAAGATAAAGTGGTCTTCACCGAGATTGCCAAATTGCTAGCTACCCATTTCGTGAAATCAAGCTGA
Protein sequence

>Msa0718730

MAPARVFGAFAGRMLMAAAAKGATKKAAASAGAASTTVVKKTAVKKTVTSKSTGGIQKVVPVTSELGNFIGAPEVSRTEAVKKVWEYIKLQNLQNPNNKKEIFCDDKLKTIFDGKDKVVFTEIAKLLATHFVKSS*