AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0823040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823040 MtrunA17_Chr5g0408111 97.037 135 3 1 1 135 1 134 4.25e-90 257
Msa0823040 MtrunA17_Chr5g0393651 47.436 78 41 0 55 132 65 142 7.28e-24 89.7
Msa0823040 MtrunA17_Chr5g0402651 45.946 74 39 1 62 135 271 343 1.44e-18 79.7
Msa0823040 MtrunA17_Chr5g0402651 42.466 73 41 1 58 130 134 205 1.35e-17 77.0
Msa0823040 MtrunA17_Chr3g0100001 41.304 92 52 1 44 133 6 97 1.26e-17 72.8
Msa0823040 MtrunA17_Chr3g0100321 37.374 99 61 1 34 132 138 235 2.20e-17 76.6
Msa0823040 MtrunA17_Chr5g0408121 37.662 77 48 0 55 131 26 102 2.48e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823040 AT2G35605.1 55.303 132 35 3 4 135 2 109 1.30e-39 129
Msa0823040 AT1G31760.1 60.606 99 38 1 37 134 13 111 2.33e-35 118
Msa0823040 AT3G03590.1 58.621 87 35 1 49 135 58 143 7.82e-32 110
Msa0823040 AT2G14880.1 43.860 114 60 1 19 132 32 141 4.56e-30 105
Msa0823040 AT4G34290.1 47.674 86 45 0 47 132 59 144 1.07e-28 102
Msa0823040 AT3G48600.1 53.165 79 36 1 54 131 33 111 9.89e-23 88.6
Msa0823040 AT3G48600.2 53.165 79 36 1 54 131 33 111 2.74e-22 88.6
Msa0823040 AT4G26810.2 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0823040 AT4G26810.1 39.362 94 55 1 44 135 6 99 5.26e-19 76.6
Msa0823040 AT4G22360.1 38.318 107 62 2 29 132 167 272 1.76e-17 77.4
Msa0823040 AT4G22360.1 36.047 86 52 2 52 135 301 385 3.29e-11 59.7
Msa0823040 AT1G49520.1 41.975 81 46 1 51 131 246 325 3.19e-15 71.2
Msa0823040 AT1G49520.1 38.272 81 49 1 51 131 97 176 2.22e-14 68.9
Msa0823040 AT3G19080.2 46.774 62 33 0 51 112 259 320 1.35e-13 66.6
Msa0823040 AT3G19080.2 38.750 80 48 1 51 130 111 189 3.06e-12 62.8
Msa0823040 AT3G19080.1 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823040 AT3G19080.1 40.260 77 43 2 60 134 385 460 1.10e-12 63.9
Msa0823040 AT3G19080.1 38.750 80 48 1 51 130 111 189 5.20e-12 62.0
Msa0823040 AT3G19080.4 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823040 AT3G19080.4 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0823040 AT3G19080.4 38.750 80 48 1 51 130 111 189 5.21e-12 62.0
Msa0823040 AT3G19080.3 46.774 62 33 0 51 112 259 320 2.31e-13 66.2
Msa0823040 AT3G19080.3 40.260 77 43 2 60 134 387 462 1.10e-12 63.9
Msa0823040 AT3G19080.3 38.750 80 48 1 51 130 111 189 5.21e-12 62.0

Find 55 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCTCCTTCTTGTTATT+AGG 0.242982 5_3:+61657855 None:intergenic
CTGAAGCTGTTAAGAAAGTT+TGG 0.251130 5_3:-61659055 Msa0823040:CDS
TTTATCTTTCCCATCGAAAA+TGG 0.302387 5_3:+61657812 None:intergenic
TATGTGTCCTCTACTTCCTT+TGG 0.328846 5_3:-61657535 Msa0823040:three_prime_UTR
GGAAGTAGAGGACACATAAC+AGG 0.342700 5_3:+61657540 None:intergenic
TTGGTGCGCCTGAAGTTTCT+AGG 0.344233 5_3:-61659079 Msa0823040:CDS
TTCAGCTTGATTTCACGAAA+TGG 0.371326 5_3:+61657754 None:intergenic
TATCAAGCTGCAGAATCTTC+AGG 0.384496 5_3:-61659027 Msa0823040:intron
AAACTTTAACTTGGGTTTGA+AGG 0.385086 5_3:-61657611 Msa0823040:three_prime_UTR
TGTAATATCAAACTTTAACT+TGG 0.404110 5_3:-61657620 Msa0823040:three_prime_UTR
TCTGAGCTCGGTAATTTCAT+TGG 0.417141 5_3:-61659098 Msa0823040:CDS
GAAATGGGTAGCTAGCAATT+TGG 0.423194 5_3:+61657770 None:intergenic
AAGAAGGCGGCTGCATCCGC+CGG 0.443418 5_3:-61659215 Msa0823040:CDS
TGAAGCTGTTAAGAAAGTTT+GGG 0.446267 5_3:-61659054 Msa0823040:CDS
CAGAATGCTAATGGCGGCGG+CGG 0.448004 5_3:-61659252 Msa0823040:CDS
ATTCGCCGGCAGAATGCTAA+TGG 0.448608 5_3:-61659261 Msa0823040:CDS
GACGGTTGTGGATGCAGCTC+CGG 0.455046 5_3:+61659196 None:intergenic
GCTTCAGTCCTAGAAACTTC+AGG 0.456584 5_3:+61659071 None:intergenic
AGGGATACAGAAGGTAGTTC+CGG 0.456709 5_3:-61659126 Msa0823040:CDS
CGCGATCATGGCACCAGCTA+GGG 0.457104 5_3:-61659294 Msa0823040:exon
GTAATATCAAACTTTAACTT+GGG 0.457408 5_3:-61657619 Msa0823040:three_prime_UTR
AAGCTGAAATTCAGCTCTGA+TGG 0.460067 5_3:-61657739 Msa0823040:exon
TCAGCTTGATTTCACGAAAT+GGG 0.467447 5_3:+61657755 None:intergenic
CTAATGGCGGCGGCGGCGAA+AGG 0.468371 5_3:-61659245 Msa0823040:CDS
AGCTAGCAATTTGGCAATCT+CGG 0.474611 5_3:+61657779 None:intergenic
GGCGAAAGGAGCCACGAAGA+AGG 0.475511 5_3:-61659231 Msa0823040:CDS
TTCGATGTTACTTCAACGGT+TGG 0.482707 5_3:+61657646 None:intergenic
CGGCAGAATGCTAATGGCGG+CGG 0.488744 5_3:-61659255 Msa0823040:CDS
GAAAACCGCCGTGAAGAAAA+CGG 0.489192 5_3:-61659168 Msa0823040:CDS
GGATGCAGCCGCCTTCTTCG+TGG 0.510322 5_3:+61659220 None:intergenic
CGCCGCCATTAGCATTCTGC+CGG 0.516612 5_3:+61659256 None:intergenic
AAATTTCGATGTTACTTCAA+CGG 0.519674 5_3:+61657642 None:intergenic
TCGCGATCATGGCACCAGCT+AGG 0.520628 5_3:-61659295 Msa0823040:exon
GCAGAATCCTAATAACAAGA+AGG 0.524697 5_3:-61657862 Msa0823040:intron
CATGCTTTAGTACATTAAGT+AGG 0.530371 5_3:-61657699 Msa0823040:three_prime_UTR
GTTCCGGTCACTTCTGAGCT+CGG 0.533521 5_3:-61659110 Msa0823040:CDS
TAAAGCATGTTACCAACGTT+AGG 0.541793 5_3:+61657713 None:intergenic
CAAAGATCCAAAGGAAGTAG+AGG 0.547155 5_3:+61657528 None:intergenic
AATGCTCCGAAAACCCTAGC+TGG 0.551067 5_3:+61659281 None:intergenic
TTTCGATGGGAAAGATAAAG+TGG 0.555020 5_3:-61657808 Msa0823040:CDS
AACTACCTTCTGTATCCCTC+CGG 0.556811 5_3:+61659130 None:intergenic
CTTTAACTTGGGTTTGAAGG+TGG 0.557831 5_3:-61657608 Msa0823040:three_prime_UTR
GAAAGGAGCCACGAAGAAGG+CGG 0.599028 5_3:-61659228 Msa0823040:CDS
CGCCGGCAGAATGCTAATGG+CGG 0.604430 5_3:-61659258 Msa0823040:CDS
GGTTGTGGATGCAGCTCCGG+CGG 0.606697 5_3:+61659199 None:intergenic
ACACAGAGTCAAAGATCCAA+AGG 0.614582 5_3:+61657519 None:intergenic
ACGGTGACGAGCAAGAGTAC+CGG 0.616079 5_3:-61659149 Msa0823040:CDS
GAACATTAGTATACGTGAGT+CGG 0.621180 5_3:-61657441 Msa0823040:three_prime_UTR
TTACCGAGCTCAGAAGTGAC+CGG 0.624475 5_3:+61659107 None:intergenic
CTCTGATGGATTCCTAACGT+TGG 0.627575 5_3:-61657725 Msa0823040:three_prime_UTR
GTGACGAGCAAGAGTACCGG+AGG 0.655721 5_3:-61659146 Msa0823040:CDS
GAAGTAGAGGACACATAACA+GGG 0.670201 5_3:+61657541 None:intergenic
ACAATGTGAAATCAAGACAG+TGG 0.670448 5_3:+61657573 None:intergenic
GAGTACCGGAGGGATACAGA+AGG 0.675343 5_3:-61659135 Msa0823040:CDS
TGACGAGCAAGAGTACCGGA+GGG 0.724885 5_3:-61659145 Msa0823040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTAATAT+CGG - chr5_3:61658310-61658329 Msa0823040:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG - chr5_3:61658387-61658406 Msa0823040:intron 10.0%
!!! TCATTTTCTTAAATTAATAT+CGG - chr5_3:61658310-61658329 Msa0823040:intron 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG - chr5_3:61658387-61658406 Msa0823040:intron 10.0%
!!! ATGTAATAATAGTTTGAAAT+CGG - chr5_3:61658451-61658470 Msa0823040:intron 15.0%
!!! ATGTAATAATAGTTTGAAAT+CGG - chr5_3:61658451-61658470 Msa0823040:intron 15.0%
!! AAGCTAAGGATATTATTTAT+AGG + chr5_3:61658642-61658661 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG - chr5_3:61658700-61658719 Msa0823040:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG - chr5_3:61658798-61658817 Msa0823040:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG - chr5_3:61658899-61658918 Msa0823040:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG - chr5_3:61659204-61659223 Msa0823040:CDS 20.0%
!!! GTAATATCAAACTTTAACTT+GGG - chr5_3:61659205-61659224 Msa0823040:CDS 20.0%
!! ATCGTTTAAAAACATCAATT+TGG + chr5_3:61659342-61659361 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG - chr5_3:61659344-61659363 Msa0823040:five_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG - chr5_3:61659345-61659364 Msa0823040:five_prime_UTR 20.0%
!! AAGCTAAGGATATTATTTAT+AGG + chr5_3:61658642-61658661 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG - chr5_3:61658700-61658719 Msa0823040:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG - chr5_3:61658798-61658817 Msa0823040:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG - chr5_3:61658899-61658918 Msa0823040:intron 20.0%
!! TGTAATATCAAACTTTAACT+TGG - chr5_3:61659204-61659223 Msa0823040:CDS 20.0%
!!! GTAATATCAAACTTTAACTT+GGG - chr5_3:61659205-61659224 Msa0823040:CDS 20.0%
!! ATCGTTTAAAAACATCAATT+TGG + chr5_3:61659342-61659361 None:intergenic 20.0%
!!! TTGATGTTTTTAAACGATAA+TGG - chr5_3:61659344-61659363 Msa0823040:five_prime_UTR 20.0%
!!! TGATGTTTTTAAACGATAAT+GGG - chr5_3:61659345-61659364 Msa0823040:five_prime_UTR 20.0%
!! AAAACTTTGATCAGGTTTTA+TGG - chr5_3:61657918-61657937 Msa0823040:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG - chr5_3:61657943-61657962 Msa0823040:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG - chr5_3:61658585-61658604 Msa0823040:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG + chr5_3:61658772-61658791 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG + chr5_3:61658776-61658795 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG + chr5_3:61658897-61658916 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG + chr5_3:61659185-61659204 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG - chr5_3:61659346-61659365 Msa0823040:five_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG - chr5_3:61659347-61659366 Msa0823040:five_prime_UTR 25.0%
!! AAAACTTTGATCAGGTTTTA+TGG - chr5_3:61657918-61657937 Msa0823040:intron 25.0%
!!! TTATTTTGCGCTATTTGATT+TGG - chr5_3:61657943-61657962 Msa0823040:intron 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG - chr5_3:61658585-61658604 Msa0823040:intron 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG + chr5_3:61658772-61658791 None:intergenic 25.0%
! GACAATTTAAGAAAGAGAAA+AGG + chr5_3:61658776-61658795 None:intergenic 25.0%
! AAGAGACAAAAATACAATAC+AGG + chr5_3:61658897-61658916 None:intergenic 25.0%
!! AAATTTCGATGTTACTTCAA+CGG + chr5_3:61659185-61659204 None:intergenic 25.0%
!!! GATGTTTTTAAACGATAATG+GGG - chr5_3:61659346-61659365 Msa0823040:five_prime_UTR 25.0%
!!! ATGTTTTTAAACGATAATGG+GGG - chr5_3:61659347-61659366 Msa0823040:five_prime_UTR 25.0%
TGAAGCTGTTAAGAAAGTTT+GGG - chr5_3:61657770-61657789 Msa0823040:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG - chr5_3:61657910-61657929 Msa0823040:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG - chr5_3:61657968-61657987 Msa0823040:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG - chr5_3:61657979-61657998 Msa0823040:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG - chr5_3:61657980-61657999 Msa0823040:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG + chr5_3:61658085-61658104 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG + chr5_3:61658285-61658304 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG - chr5_3:61658494-61658513 Msa0823040:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG - chr5_3:61658605-61658624 Msa0823040:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG + chr5_3:61658656-61658675 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG - chr5_3:61658659-61658678 Msa0823040:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG - chr5_3:61658724-61658743 Msa0823040:intron 30.0%
AGACAAAAATACAATACAGG+AGG + chr5_3:61658894-61658913 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG - chr5_3:61658904-61658923 Msa0823040:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG + chr5_3:61658972-61658991 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG + chr5_3:61659015-61659034 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG - chr5_3:61659125-61659144 Msa0823040:CDS 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG - chr5_3:61659213-61659232 Msa0823040:CDS 30.0%
TGAAGCTGTTAAGAAAGTTT+GGG - chr5_3:61657770-61657789 Msa0823040:CDS 30.0%
CTGAATGAAAAACTTTGATC+AGG - chr5_3:61657910-61657929 Msa0823040:intron 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG - chr5_3:61657968-61657987 Msa0823040:intron 30.0%
!!! TAATCGCAATGGTTTGTTTT+AGG - chr5_3:61657979-61657998 Msa0823040:intron 30.0%
!!! AATCGCAATGGTTTGTTTTA+GGG - chr5_3:61657980-61657999 Msa0823040:intron 30.0%
TTTCAAAAATCACACGAAAC+AGG + chr5_3:61658085-61658104 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG + chr5_3:61658285-61658304 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG - chr5_3:61658494-61658513 Msa0823040:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG - chr5_3:61658605-61658624 Msa0823040:intron 30.0%
AAACAAACATCTCAAAGCTA+AGG + chr5_3:61658656-61658675 None:intergenic 30.0%
!! CTTTGAGATGTTTGTTTCTT+TGG - chr5_3:61658659-61658678 Msa0823040:intron 30.0%
!!! TTTTTTTCTGCTGTTCATAC+TGG - chr5_3:61658724-61658743 Msa0823040:intron 30.0%
AGACAAAAATACAATACAGG+AGG + chr5_3:61658894-61658913 None:intergenic 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG - chr5_3:61658904-61658923 Msa0823040:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG + chr5_3:61658972-61658991 None:intergenic 30.0%
TTTATCTTTCCCATCGAAAA+TGG + chr5_3:61659015-61659034 None:intergenic 30.0%
CATGCTTTAGTACATTAAGT+AGG - chr5_3:61659125-61659144 Msa0823040:CDS 30.0%
!! AAACTTTAACTTGGGTTTGA+AGG - chr5_3:61659213-61659232 Msa0823040:CDS 30.0%
CTGAAGCTGTTAAGAAAGTT+TGG - chr5_3:61657769-61657788 Msa0823040:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG - chr5_3:61658033-61658052 Msa0823040:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG - chr5_3:61658346-61658365 Msa0823040:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG - chr5_3:61658497-61658516 Msa0823040:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG - chr5_3:61658962-61658981 Msa0823040:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG - chr5_3:61659002-61659021 Msa0823040:intron 35.0%
! AACTGAAGACCATTTTCGAT+GGG - chr5_3:61659003-61659022 Msa0823040:intron 35.0%
TTTCGATGGGAAAGATAAAG+TGG - chr5_3:61659016-61659035 Msa0823040:intron 35.0%
TCAGCTTGATTTCACGAAAT+GGG + chr5_3:61659072-61659091 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG + chr5_3:61659073-61659092 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG + chr5_3:61659114-61659133 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG + chr5_3:61659254-61659273 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG - chr5_3:61659383-61659402 Msa0823040:five_prime_UTR 35.0%
CTGAAGCTGTTAAGAAAGTT+TGG - chr5_3:61657769-61657788 Msa0823040:CDS 35.0%
! TTATTGTTGTTGCGTTCATG+AGG - chr5_3:61658033-61658052 Msa0823040:intron 35.0%
TCTGATGTTTGTGTTTGTGT+TGG - chr5_3:61658346-61658365 Msa0823040:intron 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG - chr5_3:61658497-61658516 Msa0823040:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG - chr5_3:61658962-61658981 Msa0823040:intron 35.0%
! AAACTGAAGACCATTTTCGA+TGG - chr5_3:61659002-61659021 Msa0823040:intron 35.0%
! AACTGAAGACCATTTTCGAT+GGG - chr5_3:61659003-61659022 Msa0823040:intron 35.0%
TTTCGATGGGAAAGATAAAG+TGG - chr5_3:61659016-61659035 Msa0823040:intron 35.0%
TCAGCTTGATTTCACGAAAT+GGG + chr5_3:61659072-61659091 None:intergenic 35.0%
TTCAGCTTGATTTCACGAAA+TGG + chr5_3:61659073-61659092 None:intergenic 35.0%
TAAAGCATGTTACCAACGTT+AGG + chr5_3:61659114-61659133 None:intergenic 35.0%
ACAATGTGAAATCAAGACAG+TGG + chr5_3:61659254-61659273 None:intergenic 35.0%
GAACATTAGTATACGTGAGT+CGG - chr5_3:61659383-61659402 Msa0823040:five_prime_UTR 35.0%
! CATTTTTCTCATCGCGATCA+TGG - chr5_3:61657518-61657537 Msa0823040:three_prime_UTR 40.0%
TCTGAGCTCGGTAATTTCAT+TGG - chr5_3:61657726-61657745 Msa0823040:three_prime_UTR 40.0%
TATCAAGCTGCAGAATCTTC+AGG - chr5_3:61657797-61657816 Msa0823040:CDS 40.0%
!! AGAAGTGTCAAGTGTTATCG+AGG + chr5_3:61658213-61658232 None:intergenic 40.0%
CACTTGACACTTCTGATTGA+AGG - chr5_3:61658219-61658238 Msa0823040:intron 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG + chr5_3:61658274-61658293 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG - chr5_3:61658523-61658542 Msa0823040:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG + chr5_3:61658859-61658878 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG - chr5_3:61658919-61658938 Msa0823040:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG + chr5_3:61659048-61659067 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG + chr5_3:61659057-61659076 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG - chr5_3:61659085-61659104 Msa0823040:CDS 40.0%
! TTCGATGTTACTTCAACGGT+TGG + chr5_3:61659181-61659200 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG - chr5_3:61659216-61659235 Msa0823040:CDS 40.0%
GAAGTAGAGGACACATAACA+GGG + chr5_3:61659286-61659305 None:intergenic 40.0%
TATGTGTCCTCTACTTCCTT+TGG - chr5_3:61659289-61659308 Msa0823040:CDS 40.0%
CAAAGATCCAAAGGAAGTAG+AGG + chr5_3:61659299-61659318 None:intergenic 40.0%
ACACAGAGTCAAAGATCCAA+AGG + chr5_3:61659308-61659327 None:intergenic 40.0%
! CATTTTTCTCATCGCGATCA+TGG - chr5_3:61657518-61657537 Msa0823040:three_prime_UTR 40.0%
TCTGAGCTCGGTAATTTCAT+TGG - chr5_3:61657726-61657745 Msa0823040:three_prime_UTR 40.0%
TATCAAGCTGCAGAATCTTC+AGG - chr5_3:61657797-61657816 Msa0823040:CDS 40.0%
!! AGAAGTGTCAAGTGTTATCG+AGG + chr5_3:61658213-61658232 None:intergenic 40.0%
CACTTGACACTTCTGATTGA+AGG - chr5_3:61658219-61658238 Msa0823040:intron 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG + chr5_3:61658274-61658293 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG - chr5_3:61658523-61658542 Msa0823040:intron 40.0%
ATCCAAGACAAACTATCCTG+CGG + chr5_3:61658859-61658878 None:intergenic 40.0%
TGGTGTGGCTGTTTACATAA+TGG - chr5_3:61658919-61658938 Msa0823040:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG + chr5_3:61659048-61659067 None:intergenic 40.0%
GAAATGGGTAGCTAGCAATT+TGG + chr5_3:61659057-61659076 None:intergenic 40.0%
! AAGCTGAAATTCAGCTCTGA+TGG - chr5_3:61659085-61659104 Msa0823040:CDS 40.0%
! TTCGATGTTACTTCAACGGT+TGG + chr5_3:61659181-61659200 None:intergenic 40.0%
!! CTTTAACTTGGGTTTGAAGG+TGG - chr5_3:61659216-61659235 Msa0823040:CDS 40.0%
GAAGTAGAGGACACATAACA+GGG + chr5_3:61659286-61659305 None:intergenic 40.0%
TATGTGTCCTCTACTTCCTT+TGG - chr5_3:61659289-61659308 Msa0823040:CDS 40.0%
CAAAGATCCAAAGGAAGTAG+AGG + chr5_3:61659299-61659318 None:intergenic 40.0%
ACACAGAGTCAAAGATCCAA+AGG + chr5_3:61659308-61659327 None:intergenic 40.0%
TACACACTCTCTCTCTCTCT+AGG - chr5_3:61657468-61657487 Msa0823040:three_prime_UTR 45.0%
ACACACTCTCTCTCTCTCTA+GGG - chr5_3:61657469-61657488 Msa0823040:three_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG - chr5_3:61657656-61657675 Msa0823040:three_prime_UTR 45.0%
AACTACCTTCTGTATCCCTC+CGG + chr5_3:61657697-61657716 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG - chr5_3:61657698-61657717 Msa0823040:three_prime_UTR 45.0%
GCTTCAGTCCTAGAAACTTC+AGG + chr5_3:61657756-61657775 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG - chr5_3:61658150-61658169 Msa0823040:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG + chr5_3:61658479-61658498 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG + chr5_3:61658565-61658584 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG - chr5_3:61658840-61658859 Msa0823040:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG - chr5_3:61659099-61659118 Msa0823040:CDS 45.0%
GGAAGTAGAGGACACATAAC+AGG + chr5_3:61659287-61659306 None:intergenic 45.0%
TACACACTCTCTCTCTCTCT+AGG - chr5_3:61657468-61657487 Msa0823040:three_prime_UTR 45.0%
ACACACTCTCTCTCTCTCTA+GGG - chr5_3:61657469-61657488 Msa0823040:three_prime_UTR 45.0%
GAAAACCGCCGTGAAGAAAA+CGG - chr5_3:61657656-61657675 Msa0823040:three_prime_UTR 45.0%
AACTACCTTCTGTATCCCTC+CGG + chr5_3:61657697-61657716 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG - chr5_3:61657698-61657717 Msa0823040:three_prime_UTR 45.0%
GCTTCAGTCCTAGAAACTTC+AGG + chr5_3:61657756-61657775 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG - chr5_3:61658150-61658169 Msa0823040:intron 45.0%
AAACTCTAGCCATCGCAACA+TGG + chr5_3:61658479-61658498 None:intergenic 45.0%
TAACCATCTCACCATCGCAA+GGG + chr5_3:61658565-61658584 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG - chr5_3:61658840-61658859 Msa0823040:intron 45.0%
!! CTCTGATGGATTCCTAACGT+TGG - chr5_3:61659099-61659118 Msa0823040:CDS 45.0%
GGAAGTAGAGGACACATAAC+AGG + chr5_3:61659287-61659306 None:intergenic 45.0%
! ATGGCACCAGCTAGGGTTTT+CGG - chr5_3:61657537-61657556 Msa0823040:three_prime_UTR 50.0%
AATGCTCCGAAAACCCTAGC+TGG + chr5_3:61657546-61657565 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG - chr5_3:61657563-61657582 Msa0823040:three_prime_UTR 50.0%
! GGTTTTCTTCACGACGGTTG+TGG + chr5_3:61657643-61657662 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG + chr5_3:61657667-61657686 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG + chr5_3:61657720-61657739 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG - chr5_3:61657745-61657764 Msa0823040:exon 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG - chr5_3:61658235-61658254 Msa0823040:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG + chr5_3:61658257-61658276 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG - chr5_3:61658467-61658486 Msa0823040:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG + chr5_3:61658554-61658573 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG + chr5_3:61658555-61658574 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG + chr5_3:61658566-61658585 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG - chr5_3:61658854-61658873 Msa0823040:intron 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG - chr5_3:61657537-61657556 Msa0823040:three_prime_UTR 50.0%
AATGCTCCGAAAACCCTAGC+TGG + chr5_3:61657546-61657565 None:intergenic 50.0%
ATTCGCCGGCAGAATGCTAA+TGG - chr5_3:61657563-61657582 Msa0823040:three_prime_UTR 50.0%
! GGTTTTCTTCACGACGGTTG+TGG + chr5_3:61657643-61657662 None:intergenic 50.0%
! GCTCGTCACCGTTTTCTTCA+CGG + chr5_3:61657667-61657686 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG + chr5_3:61657720-61657739 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG - chr5_3:61657745-61657764 Msa0823040:exon 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG - chr5_3:61658235-61658254 Msa0823040:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG + chr5_3:61658257-61658276 None:intergenic 50.0%
AAATCGGAGCCATGTTGCGA+TGG - chr5_3:61658467-61658486 Msa0823040:intron 50.0%
CCATCGCAAGGGCTTCAAAA+GGG + chr5_3:61658554-61658573 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG + chr5_3:61658555-61658574 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG + chr5_3:61658566-61658585 None:intergenic 50.0%
! AGCCGCAGGATAGTTTGTCT+TGG - chr5_3:61658854-61658873 Msa0823040:intron 50.0%
!! AGGGTTTTCGGAGCATTCGC+CGG - chr5_3:61657549-61657568 Msa0823040:three_prime_UTR 55.0%
GAAAGGAGCCACGAAGAAGG+CGG - chr5_3:61657596-61657615 Msa0823040:three_prime_UTR 55.0%
! CACGGCGGTTTTCTTCACGA+CGG + chr5_3:61657649-61657668 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG + chr5_3:61657664-61657683 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG - chr5_3:61657675-61657694 Msa0823040:three_prime_UTR 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG - chr5_3:61657679-61657698 Msa0823040:three_prime_UTR 55.0%
!! GAGTACCGGAGGGATACAGA+AGG - chr5_3:61657689-61657708 Msa0823040:three_prime_UTR 55.0%
GTTCCGGTCACTTCTGAGCT+CGG - chr5_3:61657714-61657733 Msa0823040:three_prime_UTR 55.0%
GTCAAACACATGCCGGACGA+TGG + chr5_3:61658250-61658269 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG - chr5_3:61658551-61658570 Msa0823040:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG - chr5_3:61658559-61658578 Msa0823040:intron 55.0%
TACGTGAGTCGGTGAGTGCA+AGG - chr5_3:61659394-61659413 Msa0823040:five_prime_UTR 55.0%
!! AGGGTTTTCGGAGCATTCGC+CGG - chr5_3:61657549-61657568 Msa0823040:three_prime_UTR 55.0%
GAAAGGAGCCACGAAGAAGG+CGG - chr5_3:61657596-61657615 Msa0823040:three_prime_UTR 55.0%
! CACGGCGGTTTTCTTCACGA+CGG + chr5_3:61657649-61657668 None:intergenic 55.0%
! CGTCACCGTTTTCTTCACGG+CGG + chr5_3:61657664-61657683 None:intergenic 55.0%
! ACGGTGACGAGCAAGAGTAC+CGG - chr5_3:61657675-61657694 Msa0823040:three_prime_UTR 55.0%
!! TGACGAGCAAGAGTACCGGA+GGG - chr5_3:61657679-61657698 Msa0823040:three_prime_UTR 55.0%
!! GAGTACCGGAGGGATACAGA+AGG - chr5_3:61657689-61657708 Msa0823040:three_prime_UTR 55.0%
GTTCCGGTCACTTCTGAGCT+CGG - chr5_3:61657714-61657733 Msa0823040:three_prime_UTR 55.0%
GTCAAACACATGCCGGACGA+TGG + chr5_3:61658250-61658269 None:intergenic 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG - chr5_3:61658551-61658570 Msa0823040:intron 55.0%
AAGCCCTTGCGATGGTGAGA+TGG - chr5_3:61658559-61658578 Msa0823040:intron 55.0%
TCGCGATCATGGCACCAGCT+AGG - chr5_3:61657529-61657548 Msa0823040:three_prime_UTR 60.0%
CGCGATCATGGCACCAGCTA+GGG - chr5_3:61657530-61657549 Msa0823040:three_prime_UTR 60.0%
CGCCGGCAGAATGCTAATGG+CGG - chr5_3:61657566-61657585 Msa0823040:three_prime_UTR 60.0%
CGCCGCCATTAGCATTCTGC+CGG + chr5_3:61657571-61657590 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG - chr5_3:61657569-61657588 Msa0823040:three_prime_UTR 60.0%
CAGAATGCTAATGGCGGCGG+CGG - chr5_3:61657572-61657591 Msa0823040:three_prime_UTR 60.0%
GGCGAAAGGAGCCACGAAGA+AGG - chr5_3:61657593-61657612 Msa0823040:three_prime_UTR 60.0%
GACGGTTGTGGATGCAGCTC+CGG + chr5_3:61657631-61657650 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG - chr5_3:61657678-61657697 Msa0823040:three_prime_UTR 60.0%
TCGCGATCATGGCACCAGCT+AGG - chr5_3:61657529-61657548 Msa0823040:three_prime_UTR 60.0%
CGCGATCATGGCACCAGCTA+GGG - chr5_3:61657530-61657549 Msa0823040:three_prime_UTR 60.0%
CGCCGGCAGAATGCTAATGG+CGG - chr5_3:61657566-61657585 Msa0823040:three_prime_UTR 60.0%
CGCCGCCATTAGCATTCTGC+CGG + chr5_3:61657571-61657590 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG - chr5_3:61657569-61657588 Msa0823040:three_prime_UTR 60.0%
CAGAATGCTAATGGCGGCGG+CGG - chr5_3:61657572-61657591 Msa0823040:three_prime_UTR 60.0%
GGCGAAAGGAGCCACGAAGA+AGG - chr5_3:61657593-61657612 Msa0823040:three_prime_UTR 60.0%
GACGGTTGTGGATGCAGCTC+CGG + chr5_3:61657631-61657650 None:intergenic 60.0%
!! GTGACGAGCAAGAGTACCGG+AGG - chr5_3:61657678-61657697 Msa0823040:three_prime_UTR 60.0%
GGATGCAGCCGCCTTCTTCG+TGG + chr5_3:61657607-61657626 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG - chr5_3:61657609-61657628 Msa0823040:three_prime_UTR 65.0%
GGTTGTGGATGCAGCTCCGG+CGG + chr5_3:61657628-61657647 None:intergenic 65.0%
GGATGCAGCCGCCTTCTTCG+TGG + chr5_3:61657607-61657626 None:intergenic 65.0%
AAGAAGGCGGCTGCATCCGC+CGG - chr5_3:61657609-61657628 Msa0823040:three_prime_UTR 65.0%
GGTTGTGGATGCAGCTCCGG+CGG + chr5_3:61657628-61657647 None:intergenic 65.0%
CTAATGGCGGCGGCGGCGAA+AGG - chr5_3:61657579-61657598 Msa0823040:three_prime_UTR 70.0%
CTAATGGCGGCGGCGGCGAA+AGG - chr5_3:61657579-61657598 Msa0823040:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 61657431 61659415 61657431 ID=Msa0823040;Name=Msa0823040
chr5_3 mRNA 61657431 61659415 61657431 ID=Msa0823040-mRNA-1;Parent=Msa0823040;Name=Msa0823040-mRNA-1;_AED=0.04;_eAED=0.04;_QI=106|1|1|1|1|1|2|324|135
chr5_3 exon 61657431 61657880 61657431 ID=Msa0823040-mRNA-1:exon:19056;Parent=Msa0823040-mRNA-1
chr5_3 exon 61659028 61659415 61659028 ID=Msa0823040-mRNA-1:exon:19055;Parent=Msa0823040-mRNA-1
chr5_3 five_prime_UTR 61659310 61659415 61659310 ID=Msa0823040-mRNA-1:five_prime_utr;Parent=Msa0823040-mRNA-1
chr5_3 CDS 61659028 61659309 61659028 ID=Msa0823040-mRNA-1:cds;Parent=Msa0823040-mRNA-1
chr5_3 CDS 61657755 61657880 61657755 ID=Msa0823040-mRNA-1:cds;Parent=Msa0823040-mRNA-1
chr5_3 three_prime_UTR 61657431 61657754 61657431 ID=Msa0823040-mRNA-1:three_prime_utr;Parent=Msa0823040-mRNA-1
Gene Sequence

>Msa0823040

ATGGCACCAGCTAGGGTTTTCGGAGCATTCGCCGGCAGAATGCTAATGGCGGCGGCGGCGAAAGGAGCCACGAAGAAGGCGGCTGCATCCGCCGGAGCTGCATCCACAACCGTCGTGAAGAAAACCGCCGTGAAGAAAACGGTGACGAGCAAGAGTACCGGAGGGATACAGAAGGTAGTTCCGGTCACTTCTGAGCTCGGTAATTTCATTGGTGCGCCTGAAGTTTCTAGGACTGAAGCTGTTAAGAAAGTTTGGGAGTATATCAAGCTGCAGAATCTTCAGAATCCTAATAACAAGAAGGAGATCTTTTGTGATGACAAACTGAAGACCATTTTCGATGGGAAAGATAAAGTGGTCTTCACCGAGATTGCCAAATTGCTAGCTACCCATTTCGTGAAATCAAGCTGA
Protein sequence

>Msa0823040

MAPARVFGAFAGRMLMAAAAKGATKKAAASAGAASTTVVKKTAVKKTVTSKSTGGIQKVVPVTSELGNFIGAPEVSRTEAVKKVWEYIKLQNLQNPNNKKEIFCDDKLKTIFDGKDKVVFTEIAKLLATHFVKSS*