AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0828890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828890 MtrunA17_Chr5g0398801 81.752 137 1 1 1 113 1 137 5.50e-72 213
Msa0828890 MtrunA17_Chr4g0011261 78.102 137 6 1 1 113 1 137 1.82e-68 204
Msa0828890 MtrunA17_Chr4g0056921 75.912 137 9 1 1 113 1 137 3.22e-67 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828890 AT4G00100.1 75.912 137 9 1 1 113 1 137 4.60e-68 203
Msa0828890 AT3G60770.1 75.182 137 10 1 1 113 1 137 3.68e-67 201

Find 62 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATACGCCCCATGGTTTC+TGG 0.160395 5_4:+1826178 None:intergenic
GGCTCATTCTTCATGCTTTC+CGG 0.179180 5_4:-1823575 Msa0828890:CDS
ACATTTCAGTGTTGCGGTAT+AGG 0.253624 5_4:-1826216 Msa0828890:five_prime_UTR
TTGGTTGCTTAGAGCTTGTA+TGG 0.287863 5_4:-1823597 Msa0828890:CDS
ATGCTTGCTGATCGCTTAGA+AGG 0.322278 5_4:-1823286 Msa0828890:three_prime_UTR
CGCCCCATGGTTTCTGGCTT+CGG 0.325578 5_4:+1826184 None:intergenic
CATTTCAGTGTTGCGGTATA+GGG 0.330127 5_4:-1826215 Msa0828890:five_prime_UTR
CTCTTGCTTGTGTTTGGGTT+GGG 0.345954 5_4:+1826294 None:intergenic
GTGCAGCAACCCTCTGTTAC+CGG 0.381872 5_4:+1823556 None:intergenic
AGAAAATTGACTTGCTCCTC+TGG 0.385566 5_4:+1823196 None:intergenic
TTACAGAAAGGCAATACAAT+AGG 0.398517 5_4:+1823227 None:intergenic
GGTTTCAATCCGCAAGCATT+TGG 0.402835 5_4:-1825027 Msa0828890:CDS
GTAACAGAGGGTTGCTGCAC+TGG 0.405636 5_4:-1823553 Msa0828890:CDS
AGGTTTATCTAGAGACTTGA+TGG 0.413371 5_4:-1823510 Msa0828890:exon
ACTCTTGCTTGTGTTTGGGT+TGG 0.418402 5_4:+1826293 None:intergenic
TATTTGCAAGTTTGCTAAGA+AGG 0.425487 5_4:-1825902 Msa0828890:CDS
AATTTATCCCTTGATTAACT+AGG 0.428497 5_4:-1826256 Msa0828890:five_prime_UTR
AAACCGCCTTCTTTATCAAA+TGG 0.432414 5_4:+1825045 None:intergenic
AACAAGGATGAGTCTGAACT+TGG 0.451307 5_4:+1824980 None:intergenic
TACAGATCCTCGGGAATCTC+AGG 0.472902 5_4:+1825068 None:intergenic
ATTTATCCCTTGATTAACTA+GGG 0.478848 5_4:-1826255 Msa0828890:five_prime_UTR
ATCAAATGGTACAGATCCTC+GGG 0.499985 5_4:+1825059 None:intergenic
ATACAAACCCTAGTTAATCA+AGG 0.505909 5_4:+1826248 None:intergenic
TGTTCCTCTCCAAATGCTTG+CGG 0.511208 5_4:+1825018 None:intergenic
ATGGGGCGTATGCATAGTAA+AGG 0.515615 5_4:-1826169 Msa0828890:CDS
TATCAAATGGTACAGATCCT+CGG 0.516683 5_4:+1825058 None:intergenic
ATATCTTTCTACTTTATCAA+AGG 0.517054 5_4:+1823459 None:intergenic
ATTTGCAAGTTTGCTAAGAA+GGG 0.526647 5_4:-1825901 Msa0828890:CDS
ATCAACAACTGCCAGCACTT+TGG 0.526669 5_4:-1823616 Msa0828890:CDS
GAAGAGATCTTAAGCCAGCT+AGG 0.528413 5_4:+1826021 None:intergenic
TGCATTTACAAATGAAACAT+AGG 0.530773 5_4:-1823326 Msa0828890:three_prime_UTR
ACAAAAGAACTTCTCCTAGC+TGG 0.534882 5_4:-1826035 Msa0828890:CDS
TAACAAAATAAGAAATTGCA+TGG 0.540677 5_4:+1823486 None:intergenic
TCTGTACCATTTGATAAAGA+AGG 0.540683 5_4:-1825051 Msa0828890:CDS
TGGTTGCTTAGAGCTTGTAT+GGG 0.548197 5_4:-1823596 Msa0828890:CDS
ATACCGCAACACTGAAATGT+TGG 0.548248 5_4:+1826219 None:intergenic
ACAGCCGAAGCCAGAAACCA+TGG 0.553216 5_4:-1826188 Msa0828890:exon
TTACCAACATTTCAGTGTTG+CGG 0.553262 5_4:-1826222 Msa0828890:five_prime_UTR
TGGGGCGTATGCATAGTAAA+GGG 0.554130 5_4:-1826168 Msa0828890:intron
CAGCCGAAGCCAGAAACCAT+GGG 0.554217 5_4:-1826187 Msa0828890:exon
TGCTGCACTGGCAATCTGCA+AGG 0.555960 5_4:-1823541 Msa0828890:CDS
GCTCTAAGCAACCAAAGTGC+TGG 0.557410 5_4:+1823605 None:intergenic
TCATGCTTTCCGGTAACAGA+GGG 0.562108 5_4:-1823565 Msa0828890:CDS
TGCTTGCTGATCGCTTAGAA+GGG 0.562937 5_4:-1823285 Msa0828890:three_prime_UTR
TTTACTATGCATACGCCCCA+TGG 0.572915 5_4:+1826171 None:intergenic
CTGGAATGCTGATTACAGAA+AGG 0.578494 5_4:+1823215 None:intergenic
GGTTTGACTCCTTCTCAGAT+CGG 0.582010 5_4:-1825880 Msa0828890:CDS
TACAAACCCTAGTTAATCAA+GGG 0.593495 5_4:+1826249 None:intergenic
AGAATAACACCGATCTGAGA+AGG 0.594247 5_4:+1825871 None:intergenic
TTCTGTAATCAGCATTCCAG+AGG 0.603700 5_4:-1823212 Msa0828890:three_prime_UTR
CAATCCGCAAGCATTTGGAG+AGG 0.603826 5_4:-1825022 Msa0828890:CDS
GGTTTATCTAGAGACTTGAT+GGG 0.612983 5_4:-1823509 Msa0828890:exon
GTACCATTTGATAAAGAAGG+CGG 0.628741 5_4:-1825048 Msa0828890:CDS
TGTAATAGCGAGCAAGACGG+TGG 0.633533 5_4:+1824946 None:intergenic
TTCATGCTTTCCGGTAACAG+AGG 0.633583 5_4:-1823566 Msa0828890:CDS
GGTGGATTCTGCTCTCAACA+AGG 0.636755 5_4:+1824964 None:intergenic
GCATTTGGAGAGGAACAGAA+AGG 0.640840 5_4:-1825012 Msa0828890:CDS
GCTTAAGATCTCTTCAGCAG+AGG 0.666834 5_4:-1826013 Msa0828890:intron
TCTTGTAATAGCGAGCAAGA+CGG 0.678094 5_4:+1824943 None:intergenic
GGAGAGGAACAGAAAGGACA+AGG 0.687113 5_4:-1825006 Msa0828890:CDS
ACTTGCACCTGAGATTCCCG+AGG 0.712102 5_4:-1825075 Msa0828890:CDS
AGCCGAAGCCAGAAACCATG+GGG 0.712434 5_4:-1826186 Msa0828890:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTATTTATCCAAAA+AGG + chr5_4:1823772-1823791 None:intergenic 10.0%
!! ATATTAAATAGAAATGTTTA+AGG - chr5_4:1823977-1823996 Msa0828890:intron 10.0%
!! TATTAAATAGAAATGTTTAA+GGG - chr5_4:1823978-1823997 Msa0828890:intron 10.0%
!!! AGAATTTCATATTTAAAATT+TGG + chr5_4:1824005-1824024 None:intergenic 10.0%
!!! TTATATTTTGAATTTGTTTC+AGG - chr5_4:1823571-1823590 Msa0828890:CDS 15.0%
!! ATTTACAAATGATAAAATAG+AGG - chr5_4:1823832-1823851 Msa0828890:intron 15.0%
!!! TAATATTTTTTTGGAGAATT+CGG + chr5_4:1823963-1823982 None:intergenic 15.0%
!! TAATGCATTAGTTAAATTTA+TGG + chr5_4:1825102-1825121 None:intergenic 15.0%
!! TAAATTTAACTAATGCATTA+TGG - chr5_4:1825102-1825121 Msa0828890:intron 15.0%
!! AAATTTAACTAATGCATTAT+GGG - chr5_4:1825103-1825122 Msa0828890:intron 15.0%
!!! ATTGAACTTATTTTAAAAAC+AGG + chr5_4:1825249-1825268 None:intergenic 15.0%
!!! AATATATCCCAATTTTTTGA+GGG - chr5_4:1824040-1824059 Msa0828890:intron 20.0%
!!! TGCTATCTTAAATATTTTGA+AGG + chr5_4:1824084-1824103 None:intergenic 20.0%
!! GAAGATTACATATGTATTTA+GGG + chr5_4:1824248-1824267 None:intergenic 20.0%
!! TGAAGATTACATATGTATTT+AGG + chr5_4:1824249-1824268 None:intergenic 20.0%
!! AAAAAAAGTAGATAATGTTG+AGG - chr5_4:1824352-1824371 Msa0828890:intron 20.0%
!!! TCATTGTTTTGTTAATATGA+AGG - chr5_4:1824408-1824427 Msa0828890:intron 20.0%
!! TTTCTTCCATTTATTTGAAA+GGG - chr5_4:1825003-1825022 Msa0828890:CDS 20.0%
!! ATGAACTCAAAATTAAAACT+TGG + chr5_4:1825199-1825218 None:intergenic 20.0%
!! TATGCTTATACTTATTTAGT+AGG + chr5_4:1825380-1825399 None:intergenic 20.0%
!! AATATAATTGGTATATGCAT+GGG + chr5_4:1825451-1825470 None:intergenic 20.0%
!! AACAGAGTAAACAATATAAT+TGG + chr5_4:1825463-1825482 None:intergenic 20.0%
!! AGCAATTTATATCAAAGTAA+CGG + chr5_4:1825515-1825534 None:intergenic 20.0%
!! TAACAAAATAAGAAATTGCA+TGG + chr5_4:1826023-1826042 None:intergenic 20.0%
!! ATATCTTTCTACTTTATCAA+AGG + chr5_4:1826050-1826069 None:intergenic 20.0%
! AATTTATCCCTTGATTAACT+AGG - chr5_4:1823250-1823269 Msa0828890:three_prime_UTR 25.0%
! ATTTATCCCTTGATTAACTA+GGG - chr5_4:1823251-1823270 Msa0828890:three_prime_UTR 25.0%
! CTCAAAAAATTGGGATATAT+TGG + chr5_4:1824041-1824060 None:intergenic 25.0%
!!! CAATATATCCCAATTTTTTG+AGG - chr5_4:1824039-1824058 Msa0828890:intron 25.0%
! TATTTGTCCCTCAAAAAATT+GGG + chr5_4:1824050-1824069 None:intergenic 25.0%
! ATATTTGTCCCTCAAAAAAT+TGG + chr5_4:1824051-1824070 None:intergenic 25.0%
! AAATACAAAACAAATGGCAA+AGG + chr5_4:1824635-1824654 None:intergenic 25.0%
! GATGGAAAATACAAAACAAA+TGG + chr5_4:1824641-1824660 None:intergenic 25.0%
! TGACATAAACAAGGTTAATT+CGG + chr5_4:1824698-1824717 None:intergenic 25.0%
! AAAAGGGAAGTAATAATCAA+AGG - chr5_4:1824943-1824962 Msa0828890:CDS 25.0%
! AAAGGGAAGTAATAATCAAA+GGG - chr5_4:1824944-1824963 Msa0828890:CDS 25.0%
! AAGTAATAATCAAAGGGTAA+AGG - chr5_4:1824950-1824969 Msa0828890:CDS 25.0%
! CTTTCTTCCATTTATTTGAA+AGG - chr5_4:1825002-1825021 Msa0828890:CDS 25.0%
! TTAAAACTTGGAAAACATGA+AGG + chr5_4:1825187-1825206 None:intergenic 25.0%
!!! TTCGTTCTAAATTTTTACTC+AGG - chr5_4:1825408-1825427 Msa0828890:intron 25.0%
! CAATATAATTGGTATATGCA+TGG + chr5_4:1825452-1825471 None:intergenic 25.0%
!! ATCAATTTTTGTTAATCACC+AGG - chr5_4:1825483-1825502 Msa0828890:intron 25.0%
!! TCAATTTTTGTTAATCACCA+GGG - chr5_4:1825484-1825503 Msa0828890:intron 25.0%
!! GCATAATATTGTTTCTCAAT+AGG - chr5_4:1825689-1825708 Msa0828890:intron 25.0%
! TTCTCAATAGGCTAATATTA+TGG - chr5_4:1825701-1825720 Msa0828890:intron 25.0%
! TGCATTTACAAATGAAACAT+AGG - chr5_4:1826180-1826199 Msa0828890:exon 25.0%
! TAGAAGGGAAAAATTTACAT+CGG - chr5_4:1826236-1826255 Msa0828890:five_prime_UTR 25.0%
! TTTTACTCTTGCTTGTGTTT+GGG + chr5_4:1823220-1823239 None:intergenic 30.0%
! TTTTTACTCTTGCTTGTGTT+TGG + chr5_4:1823221-1823240 None:intergenic 30.0%
TACAAACCCTAGTTAATCAA+GGG + chr5_4:1823260-1823279 None:intergenic 30.0%
ATACAAACCCTAGTTAATCA+AGG + chr5_4:1823261-1823280 None:intergenic 30.0%
GAAGAATGTTGAATTGAGAT+TGG + chr5_4:1823379-1823398 None:intergenic 30.0%
GAGAAGGAATAAAATTGAAG+AGG + chr5_4:1823401-1823420 None:intergenic 30.0%
!! CATTGATTTGATTGCAGTAA+GGG - chr5_4:1823425-1823444 Msa0828890:three_prime_UTR 30.0%
!! TATTTGCAAGTTTGCTAAGA+AGG - chr5_4:1823604-1823623 Msa0828890:CDS 30.0%
!! ATTTGCAAGTTTGCTAAGAA+GGG - chr5_4:1823605-1823624 Msa0828890:CDS 30.0%
!! AAGCTGTTTTCACAAGTTAT+CGG - chr5_4:1823878-1823897 Msa0828890:intron 30.0%
AAGCACTTATGAGACTATTT+GGG + chr5_4:1824184-1824203 None:intergenic 30.0%
TAAGCACTTATGAGACTATT+TGG + chr5_4:1824185-1824204 None:intergenic 30.0%
!!! ATTCATTTTTAGTCACCACA+TGG + chr5_4:1824310-1824329 None:intergenic 30.0%
CAATGAATGAATAAACTCAG+CGG + chr5_4:1824394-1824413 None:intergenic 30.0%
TCTGTACCATTTGATAAAGA+AGG - chr5_4:1824455-1824474 Msa0828890:intron 30.0%
AATCTTACTATTTCCAGACA+GGG + chr5_4:1824605-1824624 None:intergenic 30.0%
AAATCTTACTATTTCCAGAC+AGG + chr5_4:1824606-1824625 None:intergenic 30.0%
TTGTAGTTGAATCAATTGGA+TGG + chr5_4:1824659-1824678 None:intergenic 30.0%
GCATTTGTAGTTGAATCAAT+TGG + chr5_4:1824663-1824682 None:intergenic 30.0%
TTTGGCTAATGACATAAACA+AGG + chr5_4:1824707-1824726 None:intergenic 30.0%
! TTTGGAATGCCTTAAAACTT+TGG - chr5_4:1824869-1824888 Msa0828890:intron 30.0%
TTGGAATGCCTTAAAACTTT+GGG - chr5_4:1824870-1824889 Msa0828890:intron 30.0%
CCAGTAAAAAAATGGAATCT+AGG + chr5_4:1824898-1824917 None:intergenic 30.0%
!!! CCTAGATTCCATTTTTTTAC+TGG - chr5_4:1824895-1824914 Msa0828890:intron 30.0%
!!! ATTCCATTTTTTTACTGGCA+GGG - chr5_4:1824900-1824919 Msa0828890:intron 30.0%
GAATGACCCTTTCAAATAAA+TGG + chr5_4:1825012-1825031 None:intergenic 30.0%
TTGAAAGGGTCATTCATATT+TGG - chr5_4:1825017-1825036 Msa0828890:CDS 30.0%
TGAAAGGGTCATTCATATTT+GGG - chr5_4:1825018-1825037 Msa0828890:CDS 30.0%
AATATTATGGTGTGATTGCT+TGG - chr5_4:1825714-1825733 Msa0828890:intron 30.0%
AGAATTGCAAATTAACAGGA+AGG + chr5_4:1825827-1825846 None:intergenic 30.0%
TGGTAGAATTGCAAATTAAC+AGG + chr5_4:1825831-1825850 None:intergenic 30.0%
TTACAGAAAGGCAATACAAT+AGG + chr5_4:1826282-1826301 None:intergenic 30.0%
TTACCAACATTTCAGTGTTG+CGG - chr5_4:1823284-1823303 Msa0828890:three_prime_UTR 35.0%
TGTTGAATTGAGATTGGAAG+AGG + chr5_4:1823373-1823392 None:intergenic 35.0%
ATCAAATCAATGGTGTGAGA+AGG + chr5_4:1823417-1823436 None:intergenic 35.0%
CCTTACTGCAATCAAATCAA+TGG + chr5_4:1823427-1823446 None:intergenic 35.0%
!! CCATTGATTTGATTGCAGTA+AGG - chr5_4:1823424-1823443 Msa0828890:three_prime_UTR 35.0%
! AGTTCTTTTGTAGGGTAAAG+CGG + chr5_4:1823462-1823481 None:intergenic 35.0%
! CTAGGAGAAGTTCTTTTGTA+GGG + chr5_4:1823470-1823489 None:intergenic 35.0%
GTTATTCTTCGTGATTCTCA+CGG - chr5_4:1823650-1823669 Msa0828890:intron 35.0%
!! AGCTGTTTTCACAAGTTATC+GGG - chr5_4:1823879-1823898 Msa0828890:intron 35.0%
TGAGGGACAAATATTAGAAC+AGG - chr5_4:1824057-1824076 Msa0828890:intron 35.0%
GAGGGACAAATATTAGAACA+GGG - chr5_4:1824058-1824077 Msa0828890:intron 35.0%
TATCAAATGGTACAGATCCT+CGG + chr5_4:1824451-1824470 None:intergenic 35.0%
GTACCATTTGATAAAGAAGG+CGG - chr5_4:1824458-1824477 Msa0828890:intron 35.0%
AAACCGCCTTCTTTATCAAA+TGG + chr5_4:1824464-1824483 None:intergenic 35.0%
ATCTTACTATTTCCAGACAG+GGG + chr5_4:1824604-1824623 None:intergenic 35.0%
CAAAGCTCAATATCGAAGTT+TGG + chr5_4:1824725-1824744 None:intergenic 35.0%
AACTTCGATATTGAGCTTTG+TGG - chr5_4:1824725-1824744 Msa0828890:intron 35.0%
TTGAATTCGTTTGCTTGTCT+AGG - chr5_4:1824803-1824822 Msa0828890:intron 35.0%
TGAATTCGTTTGCTTGTCTA+GGG - chr5_4:1824804-1824823 Msa0828890:intron 35.0%
TGGAATGCCTTAAAACTTTG+GGG - chr5_4:1824871-1824890 Msa0828890:intron 35.0%
!!! GATTCCATTTTTTTACTGGC+AGG - chr5_4:1824899-1824918 Msa0828890:intron 35.0%
TTTCCCTGCCAGTAAAAAAA+TGG + chr5_4:1824906-1824925 None:intergenic 35.0%
TACGAGGCATGTTTACAAAA+AGG - chr5_4:1824926-1824945 Msa0828890:CDS 35.0%
ACGAGGCATGTTTACAAAAA+GGG - chr5_4:1824927-1824946 Msa0828890:CDS 35.0%
GTTAAACGTTCTATTCAGAG+AGG + chr5_4:1825141-1825160 None:intergenic 35.0%
AAAGACTTCAATGTCACTCT+TGG + chr5_4:1825333-1825352 None:intergenic 35.0%
GTAGGATAAAACACATACGA+AGG + chr5_4:1825362-1825381 None:intergenic 35.0%
CAAAAGTCATTAGTTGCGAT+AGG + chr5_4:1825858-1825877 None:intergenic 35.0%
AGGTTTATCTAGAGACTTGA+TGG - chr5_4:1825996-1826015 Msa0828890:intron 35.0%
GGTTTATCTAGAGACTTGAT+GGG - chr5_4:1825997-1826016 Msa0828890:intron 35.0%
ATACCGCAACACTGAAATGT+TGG + chr5_4:1823290-1823309 None:intergenic 40.0%
ACATTTCAGTGTTGCGGTAT+AGG - chr5_4:1823290-1823309 Msa0828890:three_prime_UTR 40.0%
CATTTCAGTGTTGCGGTATA+GGG - chr5_4:1823291-1823310 Msa0828890:three_prime_UTR 40.0%
! GCTAGGAGAAGTTCTTTTGT+AGG + chr5_4:1823471-1823490 None:intergenic 40.0%
ACAAAAGAACTTCTCCTAGC+TGG - chr5_4:1823471-1823490 Msa0828890:three_prime_UTR 40.0%
AGAATAACACCGATCTGAGA+AGG + chr5_4:1823638-1823657 None:intergenic 40.0%
!! CTCTCTATGCTGTGTTGTTT+CGG - chr5_4:1823736-1823755 Msa0828890:intron 40.0%
!! CTGTGTTGTTTCGGTTTGTT+AGG - chr5_4:1823745-1823764 Msa0828890:intron 40.0%
!!! TCGGTTTGTTAGGTCCTTTT+TGG - chr5_4:1823755-1823774 Msa0828890:intron 40.0%
!! GCTGTTTTCACAAGTTATCG+GGG - chr5_4:1823880-1823899 Msa0828890:intron 40.0%
!! ACCTGTTCGTAAGCTGTTTT+TGG + chr5_4:1824141-1824160 None:intergenic 40.0%
CCACATGGACACAAATTCAT+AGG + chr5_4:1824295-1824314 None:intergenic 40.0%
CCTATGAATTTGTGTCCATG+TGG - chr5_4:1824292-1824311 Msa0828890:intron 40.0%
ATCAAATGGTACAGATCCTC+GGG + chr5_4:1824450-1824469 None:intergenic 40.0%
AACAAGGATGAGTCTGAACT+TGG + chr5_4:1824529-1824548 None:intergenic 40.0%
TCTTGTAATAGCGAGCAAGA+CGG + chr5_4:1824566-1824585 None:intergenic 40.0%
TCTTACTATTTCCAGACAGG+GGG + chr5_4:1824603-1824622 None:intergenic 40.0%
! GTTAATTCGGTGACTAAGAC+TGG + chr5_4:1824685-1824704 None:intergenic 40.0%
! TTCGATATTGAGCTTTGTGG+TGG - chr5_4:1824728-1824747 Msa0828890:intron 40.0%
GTGGTATGCATTAGATCGAT+CGG - chr5_4:1824747-1824766 Msa0828890:intron 40.0%
GAATTCGTTTGCTTGTCTAG+GGG - chr5_4:1824805-1824824 Msa0828890:intron 40.0%
! TCTAGGTCCCCAAAGTTTTA+AGG + chr5_4:1824881-1824900 None:intergenic 40.0%
TGGGTCCTAGTGAACTTTAT+GGG + chr5_4:1825047-1825066 None:intergenic 40.0%
ATGGGTCCTAGTGAACTTTA+TGG + chr5_4:1825048-1825067 None:intergenic 40.0%
AATAAGTTCAATTGCGTGCC+TGG - chr5_4:1825257-1825276 Msa0828890:intron 40.0%
CTGGTTGACTAGATGAAAAG+CGG - chr5_4:1825276-1825295 Msa0828890:intron 40.0%
TCAAAGTAACGGTAAAACCC+TGG + chr5_4:1825504-1825523 None:intergenic 40.0%
GCACAGCAACATGAATGAAA+TGG - chr5_4:1825573-1825592 Msa0828890:intron 40.0%
GCAAGACTAGAAAGTTCAAG+AGG + chr5_4:1825805-1825824 None:intergenic 40.0%
CATTAGTTGCGATAGGAACA+TGG + chr5_4:1825851-1825870 None:intergenic 40.0%
TTGGTTGCTTAGAGCTTGTA+TGG - chr5_4:1825909-1825928 Msa0828890:CDS 40.0%
TGGTTGCTTAGAGCTTGTAT+GGG - chr5_4:1825910-1825929 Msa0828890:CDS 40.0%
!! CAATCTGCAAGGTCATTTTG+AGG - chr5_4:1825976-1825995 Msa0828890:intron 40.0%
! CTGGAATGCTGATTACAGAA+AGG + chr5_4:1826294-1826313 None:intergenic 40.0%
TTCTGTAATCAGCATTCCAG+AGG - chr5_4:1826294-1826313 Msa0828890:five_prime_UTR 40.0%
!! AGAAAATTGACTTGCTCCTC+TGG + chr5_4:1826313-1826332 None:intergenic 40.0%
! CTCTTGCTTGTGTTTGGGTT+GGG + chr5_4:1823215-1823234 None:intergenic 45.0%
ACTCTTGCTTGTGTTTGGGT+TGG + chr5_4:1823216-1823235 None:intergenic 45.0%
TTTACTATGCATACGCCCCA+TGG + chr5_4:1823338-1823357 None:intergenic 45.0%
ATGGGGCGTATGCATAGTAA+AGG - chr5_4:1823337-1823356 Msa0828890:three_prime_UTR 45.0%
TGGGGCGTATGCATAGTAAA+GGG - chr5_4:1823338-1823357 Msa0828890:three_prime_UTR 45.0%
! TCTTTTGTAGGGTAAAGCGG+AGG + chr5_4:1823459-1823478 None:intergenic 45.0%
GAAGAGATCTTAAGCCAGCT+AGG + chr5_4:1823488-1823507 None:intergenic 45.0%
GCTTAAGATCTCTTCAGCAG+AGG - chr5_4:1823493-1823512 Msa0828890:three_prime_UTR 45.0%
GGTTTGACTCCTTCTCAGAT+CGG - chr5_4:1823626-1823645 Msa0828890:intron 45.0%
!! TACCGTGAGCTTTGAGGATA+CGG + chr5_4:1823715-1823734 None:intergenic 45.0%
! TTTTCACAAGTTATCGGGGC+TGG - chr5_4:1823884-1823903 Msa0828890:intron 45.0%
TTCGGCACCTCAAAATTTCG+AGG + chr5_4:1823945-1823964 None:intergenic 45.0%
GATAGCATTGCAAGTTACGC+TGG - chr5_4:1824097-1824116 Msa0828890:intron 45.0%
GCCAAAAACAGCTTACGAAC+AGG - chr5_4:1824137-1824156 Msa0828890:intron 45.0%
GGTTTCAATCCGCAAGCATT+TGG - chr5_4:1824479-1824498 Msa0828890:intron 45.0%
TGTTCCTCTCCAAATGCTTG+CGG + chr5_4:1824491-1824510 None:intergenic 45.0%
GCATTTGGAGAGGAACAGAA+AGG - chr5_4:1824494-1824513 Msa0828890:intron 45.0%
TTTACTGGCAGGGAAATACG+AGG - chr5_4:1824910-1824929 Msa0828890:intron 45.0%
GGCATCCCATAAAGTTCACT+AGG - chr5_4:1825039-1825058 Msa0828890:CDS 45.0%
ACAAGATCCTTCCTAAGTGC+AGG + chr5_4:1825076-1825095 None:intergenic 45.0%
ACGTTTAACATCCTTCCCCT+TGG - chr5_4:1825152-1825171 Msa0828890:intron 45.0%
CGTTTAACATCCTTCCCCTT+GGG - chr5_4:1825153-1825172 Msa0828890:intron 45.0%
ATGAAGGAAGATGCACCCAA+GGG + chr5_4:1825171-1825190 None:intergenic 45.0%
GACTAGAGCTGAAGATGACA+CGG + chr5_4:1825223-1825242 None:intergenic 45.0%
! CGCTTTTCATCTAGTCAACC+AGG + chr5_4:1825278-1825297 None:intergenic 45.0%
ATCAACAACTGCCAGCACTT+TGG - chr5_4:1825890-1825909 Msa0828890:CDS 45.0%
GGCTCATTCTTCATGCTTTC+CGG - chr5_4:1825931-1825950 Msa0828890:intron 45.0%
TTCATGCTTTCCGGTAACAG+AGG - chr5_4:1825940-1825959 Msa0828890:intron 45.0%
TCATGCTTTCCGGTAACAGA+GGG - chr5_4:1825941-1825960 Msa0828890:intron 45.0%
! ATGCTTGCTGATCGCTTAGA+AGG - chr5_4:1826220-1826239 Msa0828890:five_prime_UTR 45.0%
! TGCTTGCTGATCGCTTAGAA+GGG - chr5_4:1826221-1826240 Msa0828890:five_prime_UTR 45.0%
!!! ATTTCTATTTAATATTTTTT+TGG + chr5_4:1823972-1823991 None:intergenic 5.0%
!!! ATTAAAAATTAATTAAGTTT+TGG - chr5_4:1824851-1824870 Msa0828890:intron 5.0%
TGATTCTCACGGCATTGCTC+AGG - chr5_4:1823661-1823680 Msa0828890:intron 50.0%
! GATACGGAGGATTTTGCTGC+CGG + chr5_4:1823699-1823718 None:intergenic 50.0%
CTCCGTATCCTCAAAGCTCA+CGG - chr5_4:1823710-1823729 Msa0828890:intron 50.0%
GAGAGATACCGTGAGCTTTG+AGG + chr5_4:1823721-1823740 None:intergenic 50.0%
TGTAGTATCCCGCTTTGCAC+AGG + chr5_4:1823917-1823936 None:intergenic 50.0%
TGCAAAGCGGGATACTACAC+AGG - chr5_4:1823918-1823937 Msa0828890:intron 50.0%
GCAAAGCGGGATACTACACA+GGG - chr5_4:1823919-1823938 Msa0828890:intron 50.0%
! CACAGGGCCTCGAAATTTTG+AGG - chr5_4:1823935-1823954 Msa0828890:intron 50.0%
TACAGATCCTCGGGAATCTC+AGG + chr5_4:1824441-1824460 None:intergenic 50.0%
CAATCCGCAAGCATTTGGAG+AGG - chr5_4:1824484-1824503 Msa0828890:intron 50.0%
GGAGAGGAACAGAAAGGACA+AGG - chr5_4:1824500-1824519 Msa0828890:intron 50.0%
! GGTGGATTCTGCTCTCAACA+AGG + chr5_4:1824545-1824564 None:intergenic 50.0%
TGTAATAGCGAGCAAGACGG+TGG + chr5_4:1824563-1824582 None:intergenic 50.0%
TCCTAAGTGCAGGTGCTCAT+GGG + chr5_4:1825066-1825085 None:intergenic 50.0%
TTCCTAAGTGCAGGTGCTCA+TGG + chr5_4:1825067-1825086 None:intergenic 50.0%
ATGAGCACCTGCACTTAGGA+AGG - chr5_4:1825066-1825085 Msa0828890:CDS 50.0%
TGAAGGAAGATGCACCCAAG+GGG + chr5_4:1825170-1825189 None:intergenic 50.0%
CATGAAGGAAGATGCACCCA+AGG + chr5_4:1825172-1825191 None:intergenic 50.0%
! ATGTTGCTGTGCAGAAGCAC+GGG + chr5_4:1825565-1825584 None:intergenic 50.0%
CATGTTGCTGTGCAGAAGCA+CGG + chr5_4:1825566-1825585 None:intergenic 50.0%
! GCTCTAAGCAACCAAAGTGC+TGG + chr5_4:1825904-1825923 None:intergenic 50.0%
ACAGCCGAAGCCAGAAACCA+TGG - chr5_4:1823318-1823337 Msa0828890:three_prime_UTR 55.0%
CAGCCGAAGCCAGAAACCAT+GGG - chr5_4:1823319-1823338 Msa0828890:three_prime_UTR 55.0%
AGCCGAAGCCAGAAACCATG+GGG - chr5_4:1823320-1823339 Msa0828890:three_prime_UTR 55.0%
TGCATACGCCCCATGGTTTC+TGG + chr5_4:1823331-1823350 None:intergenic 55.0%
!! GCTCAGGTCAAGAGCGTTAC+CGG - chr5_4:1823677-1823696 Msa0828890:intron 55.0%
!! CGTGAGCTTTGAGGATACGG+AGG + chr5_4:1823712-1823731 None:intergenic 55.0%
GGTGAACAGCCTGTGCAAAG+CGG - chr5_4:1823905-1823924 Msa0828890:intron 55.0%
GTGAACAGCCTGTGCAAAGC+GGG - chr5_4:1823906-1823925 Msa0828890:intron 55.0%
ACTTGCACCTGAGATTCCCG+AGG - chr5_4:1824431-1824450 Msa0828890:intron 55.0%
ACCCATGAGCACCTGCACTT+AGG - chr5_4:1825062-1825081 Msa0828890:CDS 55.0%
GTGCAGCAACCCTCTGTTAC+CGG + chr5_4:1825953-1825972 None:intergenic 55.0%
GTAACAGAGGGTTGCTGCAC+TGG - chr5_4:1825953-1825972 Msa0828890:intron 55.0%
! TGCTGCACTGGCAATCTGCA+AGG - chr5_4:1825965-1825984 Msa0828890:intron 55.0%
!! CGCCCCATGGTTTCTGGCTT+CGG + chr5_4:1823325-1823344 None:intergenic 60.0%
CCAGACAGGGGGAAGCTTCT+TGG + chr5_4:1824592-1824611 None:intergenic 60.0%
CCAAGAAGCTTCCCCCTGTC+TGG - chr5_4:1824589-1824608 Msa0828890:intron 60.0%
GGAAGATGCACCCAAGGGGA+AGG + chr5_4:1825166-1825185 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 1823195 1826333 1823195 ID=Msa0828890;Name=Msa0828890
chr5_4 mRNA 1823195 1826333 1823195 ID=Msa0828890-mRNA-1;Parent=Msa0828890;Name=Msa0828890-mRNA-1;_AED=0.07;_eAED=0.07;_QI=142|0.5|0.6|1|1|1|5|318|155
chr5_4 exon 1823195 1823641 1823195 ID=Msa0828890-mRNA-1:exon:503;Parent=Msa0828890-mRNA-1
chr5_4 exon 1824923 1825098 1824923 ID=Msa0828890-mRNA-1:exon:502;Parent=Msa0828890-mRNA-1
chr5_4 exon 1825869 1825935 1825869 ID=Msa0828890-mRNA-1:exon:501;Parent=Msa0828890-mRNA-1
chr5_4 exon 1826014 1826086 1826014 ID=Msa0828890-mRNA-1:exon:500;Parent=Msa0828890-mRNA-1
chr5_4 exon 1826169 1826333 1826169 ID=Msa0828890-mRNA-1:exon:499;Parent=Msa0828890-mRNA-1
chr5_4 five_prime_UTR 1826192 1826333 1826192 ID=Msa0828890-mRNA-1:five_prime_utr;Parent=Msa0828890-mRNA-1
chr5_4 CDS 1826169 1826191 1826169 ID=Msa0828890-mRNA-1:cds;Parent=Msa0828890-mRNA-1
chr5_4 CDS 1826014 1826086 1826014 ID=Msa0828890-mRNA-1:cds;Parent=Msa0828890-mRNA-1
chr5_4 CDS 1825869 1825935 1825869 ID=Msa0828890-mRNA-1:cds;Parent=Msa0828890-mRNA-1
chr5_4 CDS 1824923 1825098 1824923 ID=Msa0828890-mRNA-1:cds;Parent=Msa0828890-mRNA-1
chr5_4 CDS 1823513 1823641 1823513 ID=Msa0828890-mRNA-1:cds;Parent=Msa0828890-mRNA-1
chr5_4 three_prime_UTR 1823195 1823512 1823195 ID=Msa0828890-mRNA-1:three_prime_utr;Parent=Msa0828890-mRNA-1
Gene Sequence

>Msa0828890

ATGGGGCGTATGCATAGTAAAGGTAAGGGTATTTCTTCCTCCGCTTTACCCTACAAAAGAACTTCTCCTAGCTGGCTTAAGATCTCTTCAGCAGAGGTTGATGAGACTATTTGCAAGTTTGCTAAGAAGGGTTTGACTCCTTCTCAGATCGGTGTTATTCTTCGACTTGCACCTGAGATTCCCGAGGATCTGTACCATTTGATAAAGAAGGCGGTTTCAATCCGCAAGCATTTGGAGAGGAACAGAAAGGACAAGGACTCCAAGTTCAGACTCATCCTTGTTGAGAGCAGAATCCACCGTCTTGCTCGCTATTACAAGAAAACCAAGAAGCTTCCCCCTCGAATCAACAACTGCCAGCACTTTGGTTGCTTAGAGCTTGTATGGGCTCATTCTTCATGCTTTCCGGTAACAGAGGGTTGCTGCACTGGCAATCTGCAAGGTCATTTTGAGGTTTATCTAGAGACTTGA
Protein sequence

>Msa0828890

MGRMHSKGKGISSSALPYKRTSPSWLKISSAEVDETICKFAKKGLTPSQIGVILRLAPEIPEDLYHLIKKAVSIRKHLERNRKDKDSKFRLILVESRIHRLARYYKKTKKLPPRINNCQHFGCLELVWAHSSCFPVTEGCCTGNLQGHFEVYLET*