AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0829520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 81 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATAAATACATGATTTC+TGG 0.146830 5_4:-2518115 Msa0829520:five_prime_UTR
GACATTAATTATACATAATT+AGG 0.147476 5_4:+2516768 None:intergenic
TTATACATAATTAGGGATTT+AGG 0.224823 5_4:+2516776 None:intergenic
TATTATACTCCAGCTGATTA+TGG 0.249358 5_4:-2516992 Msa0829520:CDS
TTGTAATTTATGTCTGCTTT+TGG 0.257013 5_4:-2516803 Msa0829520:three_prime_UTR
CTCTAGTAGTTTGCGGCTTT+GGG 0.258315 5_4:-2516846 Msa0829520:three_prime_UTR
TCTCTAGTAGTTTGCGGCTT+TGG 0.263573 5_4:-2516847 Msa0829520:three_prime_UTR
ACATAAATTACAATACTAAT+AGG 0.269901 5_4:+2516813 None:intergenic
TCAAAGCTTTGACAACAGTA+TGG 0.296747 5_4:-2517348 Msa0829520:CDS
CTTTCTCTCGCCAAACGTTC+GGG 0.317643 5_4:+2517514 None:intergenic
TAATGGGCCGGACAATGATT+TGG 0.326894 5_4:-2518200 Msa0829520:five_prime_UTR
ACATTAATTATACATAATTA+GGG 0.355027 5_4:+2516769 None:intergenic
AAAACAATGGAGAGAGAAAA+AGG 0.360858 5_4:+2517629 None:intergenic
TCTTTCTCTCGCCAAACGTT+CGG 0.363000 5_4:+2517513 None:intergenic
GAAGGTGAAAGAAAGAATAA+TGG 0.363979 5_4:-2518217 Msa0829520:five_prime_UTR
TGTCCACCACAATTCTCTTT+CGG 0.378035 5_4:+2518176 None:intergenic
TCCAGCTGATTATGGATTAG+AGG 0.379886 5_4:-2516984 Msa0829520:CDS
CCTGGGCGCACAACAACAAT+TGG 0.389088 5_4:+2517048 None:intergenic
GCGCCCAGGGTGCAGCCTCT+TGG 0.396019 5_4:-2517034 Msa0829520:CDS
AACTTATCTCTAGTAGTTTG+CGG 0.398770 5_4:-2516853 Msa0829520:three_prime_UTR
GTACTCAAATCGGTCTTCAT+AGG 0.417050 5_4:+2517093 None:intergenic
CCAATTGTTGTTGTGCGCCC+AGG 0.417668 5_4:-2517048 Msa0829520:CDS
AAAACAAAGTTGTCATCAAA+TGG 0.421430 5_4:-2517587 Msa0829520:five_prime_UTR
AATGGGCCGGACAATGATTT+GGG 0.427175 5_4:-2518199 Msa0829520:five_prime_UTR
TACTCCTTACTAATACTACT+AGG 0.430270 5_4:+2516886 None:intergenic
GGGCCAAGAGGCTGCACCCT+GGG 0.431419 5_4:+2517031 None:intergenic
AAGGTGAAAGAAAGAATAAT+GGG 0.433797 5_4:-2518216 Msa0829520:five_prime_UTR
AACAACATCATGTGAGTATG+AGG 0.438895 5_4:+2517664 None:intergenic
ATGAAGACCGATTTGAGTAC+TGG 0.440283 5_4:-2517090 Msa0829520:CDS
GACTACCTTCCAACTCAGTA+TGG 0.445544 5_4:-2517127 Msa0829520:CDS
CATAAATTACAATACTAATA+GGG 0.445876 5_4:+2516814 None:intergenic
TCTATAAATACATGATTTCT+GGG 0.446078 5_4:-2518114 Msa0829520:five_prime_UTR
CGATAATATGTCCAATGAAA+AGG 0.453370 5_4:-2517452 Msa0829520:CDS
AATCCCTAGTAGTATTAGTA+AGG 0.479237 5_4:-2516890 Msa0829520:three_prime_UTR
TCCTCTAATCCATAATCAGC+TGG 0.484339 5_4:+2516983 None:intergenic
TTTCGGCCCAAATCATTGTC+CGG 0.489949 5_4:+2518193 None:intergenic
AGCGATCCCAGTACTCAAAT+CGG 0.492770 5_4:+2517083 None:intergenic
AGTAAATAACAGTTAAGAGC+AGG 0.493569 5_4:+2516744 None:intergenic
CAAAGCTTTGACAACAGTAT+GGG 0.494576 5_4:-2517347 Msa0829520:CDS
AAGCAAGCAACCTATTCTCT+TGG 0.499131 5_4:+2518056 None:intergenic
CAATTGTTGTTGTGCGCCCA+GGG 0.500706 5_4:-2517047 Msa0829520:CDS
CCCTGGTGATATCATGTTCG+AGG 0.505379 5_4:-2517149 Msa0829520:CDS
GATTAGAGGATGATGATTGA+TGG 0.509050 5_4:-2516970 Msa0829520:exon
TACAAATTATTTACCCGGGT+CGG 0.521216 5_4:+2518150 None:intergenic
TAATAACTAGCTGGACGTTG+TGG 0.523669 5_4:+2517010 None:intergenic
AATAACTAGCTGGACGTTGT+GGG 0.528802 5_4:+2517011 None:intergenic
TTCAAAACATGAATCAGACT+TGG 0.535772 5_4:-2517314 Msa0829520:CDS
TGGGCCAAGAGGCTGCACCC+TGG 0.540694 5_4:+2517030 None:intergenic
TCCTCGAACATGATATCACC+AGG 0.543019 5_4:+2517148 None:intergenic
GCTGGAGTATAATAACTAGC+TGG 0.548205 5_4:+2517001 None:intergenic
GTATTTATAGAGCGGCCGCT+TGG 0.554556 5_4:+2518126 None:intergenic
ACTCCTTACTAATACTACTA+GGG 0.554568 5_4:+2516887 None:intergenic
GTTAAGAGTGCGTATGAGAA+GGG 0.563631 5_4:-2517274 Msa0829520:CDS
TGTTAAGAGTGCGTATGAGA+AGG 0.565399 5_4:-2517275 Msa0829520:CDS
TTGGTTACAAATTATTTACC+CGG 0.577076 5_4:+2518145 None:intergenic
TTGAGCCTACCATACTGAGT+TGG 0.578647 5_4:+2517118 None:intergenic
ACAACAACAATTGGCACGCG+TGG 0.596401 5_4:+2517057 None:intergenic
TGAAAGAAAGAATAATGGGC+CGG 0.596944 5_4:-2518212 Msa0829520:five_prime_UTR
AACCTCGTAACTGTTGCCAC+TGG 0.597504 5_4:+2517912 None:intergenic
CTTACATCTCACTAAAATCA+CGG 0.598432 5_4:+2517248 None:intergenic
TGAAGACCGATTTGAGTACT+GGG 0.608071 5_4:-2517089 Msa0829520:CDS
AACTCAGTATGGTAGGCTCA+AGG 0.609591 5_4:-2517116 Msa0829520:CDS
TGTGGTGGACATAACCGACC+CGG 0.621203 5_4:-2518164 Msa0829520:five_prime_UTR
CTTGATCTTGATAATGACGT+GGG 0.622967 5_4:-2517406 Msa0829520:CDS
TGAGTAATGAAAATGAAAGA+AGG 0.630657 5_4:-2518235 None:intergenic
TTCCAGTGGCAACAGTTACG+AGG 0.632420 5_4:-2517914 Msa0829520:five_prime_UTR
GGGCCGAAAGAGAATTGTGG+TGG 0.634470 5_4:-2518179 Msa0829520:five_prime_UTR
ACCTTCCAACTCAGTATGGT+AGG 0.634786 5_4:-2517123 Msa0829520:CDS
GAAATCATGTATTTATAGAG+CGG 0.640417 5_4:+2518118 None:intergenic
AAAGCTTTGACAACAGTATG+GGG 0.642690 5_4:-2517346 Msa0829520:CDS
ACAACATCATGTGAGTATGA+GGG 0.648593 5_4:+2517665 None:intergenic
TGGTTACAAATTATTTACCC+GGG 0.649137 5_4:+2518146 None:intergenic
TCTTGATCTTGATAATGACG+TGG 0.649200 5_4:-2517407 Msa0829520:CDS
GTGGTGGACATAACCGACCC+GGG 0.650786 5_4:-2518163 Msa0829520:five_prime_UTR
GCCTACCATACTGAGTTGGA+AGG 0.655535 5_4:+2517122 None:intergenic
GCTGGACGTTGTGGGCCAAG+AGG 0.661359 5_4:+2517019 None:intergenic
GTAAATAATTTGTAACCAAG+CGG 0.664321 5_4:-2518141 Msa0829520:five_prime_UTR
AAGAGTGCGTATGAGAAGGG+CGG 0.697112 5_4:-2517271 Msa0829520:CDS
CTTCACATAATGTCTTCCAG+TGG 0.702452 5_4:-2517928 Msa0829520:five_prime_UTR
TTTGGGCCGAAAGAGAATTG+TGG 0.711693 5_4:-2518182 Msa0829520:five_prime_UTR
CCTCGAACATGATATCACCA+GGG 0.756060 5_4:+2517149 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGATAATAAAACATTAA+CGG + chr5_4:2517758-2517777 None:intergenic 10.0%
!! ACATTAATTATACATAATTA+GGG + chr5_4:2518184-2518203 None:intergenic 10.0%
!! ATTAGATAATAAAACATTAA+CGG + chr5_4:2517758-2517777 None:intergenic 10.0%
!! ACATTAATTATACATAATTA+GGG + chr5_4:2518184-2518203 None:intergenic 10.0%
!! ACATAGTATATTAGATAAAA+GGG + chr5_4:2516964-2516983 None:intergenic 15.0%
!! AACATAGTATATTAGATAAA+AGG + chr5_4:2516965-2516984 None:intergenic 15.0%
!!! AATCACTTTTTGATAAATTT+TGG - chr5_4:2517252-2517271 Msa0829520:intron 15.0%
!! CATAAATTACAATACTAATA+GGG + chr5_4:2518139-2518158 None:intergenic 15.0%
!! ACATAAATTACAATACTAAT+AGG + chr5_4:2518140-2518159 None:intergenic 15.0%
!! GACATTAATTATACATAATT+AGG + chr5_4:2518185-2518204 None:intergenic 15.0%
!! ACATAGTATATTAGATAAAA+GGG + chr5_4:2516964-2516983 None:intergenic 15.0%
!! AACATAGTATATTAGATAAA+AGG + chr5_4:2516965-2516984 None:intergenic 15.0%
!!! AATCACTTTTTGATAAATTT+TGG - chr5_4:2517252-2517271 Msa0829520:intron 15.0%
!! CATAAATTACAATACTAATA+GGG + chr5_4:2518139-2518158 None:intergenic 15.0%
!! ACATAAATTACAATACTAAT+AGG + chr5_4:2518140-2518159 None:intergenic 15.0%
!! GACATTAATTATACATAATT+AGG + chr5_4:2518185-2518204 None:intergenic 15.0%
!! TCTATAAATACATGATTTCT+GGG - chr5_4:2516836-2516855 Msa0829520:three_prime_UTR 20.0%
!! TTATACATAATTAGGGATTT+AGG + chr5_4:2518177-2518196 None:intergenic 20.0%
!! TCTATAAATACATGATTTCT+GGG - chr5_4:2516836-2516855 Msa0829520:three_prime_UTR 20.0%
!! TTATACATAATTAGGGATTT+AGG + chr5_4:2518177-2518196 None:intergenic 20.0%
! AAGGTGAAAGAAAGAATAAT+GGG - chr5_4:2516734-2516753 Msa0829520:three_prime_UTR 25.0%
! TTGGTTACAAATTATTTACC+CGG + chr5_4:2516808-2516827 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG - chr5_4:2516809-2516828 Msa0829520:three_prime_UTR 25.0%
! GAAATCATGTATTTATAGAG+CGG + chr5_4:2516835-2516854 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG - chr5_4:2516835-2516854 Msa0829520:three_prime_UTR 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG - chr5_4:2516884-2516903 Msa0829520:three_prime_UTR 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr5_4:2517337-2517356 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr5_4:2517350-2517369 None:intergenic 25.0%
! AAAACAAAGTTGTCATCAAA+TGG - chr5_4:2517363-2517382 Msa0829520:CDS 25.0%
!! TTGTAATTTATGTCTGCTTT+TGG - chr5_4:2518147-2518166 Msa0829520:five_prime_UTR 25.0%
!!! TGTAATTTATGTCTGCTTTT+GGG - chr5_4:2518148-2518167 Msa0829520:five_prime_UTR 25.0%
! AAGGTGAAAGAAAGAATAAT+GGG - chr5_4:2516734-2516753 Msa0829520:three_prime_UTR 25.0%
! TTGGTTACAAATTATTTACC+CGG + chr5_4:2516808-2516827 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG - chr5_4:2516809-2516828 Msa0829520:three_prime_UTR 25.0%
! GAAATCATGTATTTATAGAG+CGG + chr5_4:2516835-2516854 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG - chr5_4:2516835-2516854 Msa0829520:three_prime_UTR 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG - chr5_4:2516884-2516903 Msa0829520:three_prime_UTR 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr5_4:2517337-2517356 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr5_4:2517350-2517369 None:intergenic 25.0%
! AAAACAAAGTTGTCATCAAA+TGG - chr5_4:2517363-2517382 Msa0829520:CDS 25.0%
!! TTGTAATTTATGTCTGCTTT+TGG - chr5_4:2518147-2518166 Msa0829520:five_prime_UTR 25.0%
!!! TGTAATTTATGTCTGCTTTT+GGG - chr5_4:2518148-2518167 Msa0829520:five_prime_UTR 25.0%
GAAGGTGAAAGAAAGAATAA+TGG - chr5_4:2516733-2516752 Msa0829520:three_prime_UTR 30.0%
TGGTTACAAATTATTTACCC+GGG + chr5_4:2516807-2516826 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr5_4:2517324-2517343 None:intergenic 30.0%
CGATAATATGTCCAATGAAA+AGG - chr5_4:2517498-2517517 Msa0829520:five_prime_UTR 30.0%
TTCAAAACATGAATCAGACT+TGG - chr5_4:2517636-2517655 Msa0829520:five_prime_UTR 30.0%
CTTACATCTCACTAAAATCA+CGG + chr5_4:2517705-2517724 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG + chr5_4:2517785-2517804 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG - chr5_4:2517784-2517803 Msa0829520:five_prime_UTR 30.0%
TATTATACTCCAGCTGATTA+TGG - chr5_4:2517958-2517977 Msa0829520:intron 30.0%
AATCCCTAGTAGTATTAGTA+AGG - chr5_4:2518060-2518079 Msa0829520:intron 30.0%
ACTCCTTACTAATACTACTA+GGG + chr5_4:2518066-2518085 None:intergenic 30.0%
TACTCCTTACTAATACTACT+AGG + chr5_4:2518067-2518086 None:intergenic 30.0%
AACTTATCTCTAGTAGTTTG+CGG - chr5_4:2518097-2518116 Msa0829520:five_prime_UTR 30.0%
AGTAAATAACAGTTAAGAGC+AGG + chr5_4:2518209-2518228 None:intergenic 30.0%
GAAGGTGAAAGAAAGAATAA+TGG - chr5_4:2516733-2516752 Msa0829520:three_prime_UTR 30.0%
TGGTTACAAATTATTTACCC+GGG + chr5_4:2516807-2516826 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr5_4:2517324-2517343 None:intergenic 30.0%
CGATAATATGTCCAATGAAA+AGG - chr5_4:2517498-2517517 Msa0829520:five_prime_UTR 30.0%
TTCAAAACATGAATCAGACT+TGG - chr5_4:2517636-2517655 Msa0829520:five_prime_UTR 30.0%
CTTACATCTCACTAAAATCA+CGG + chr5_4:2517705-2517724 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG + chr5_4:2517785-2517804 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG - chr5_4:2517784-2517803 Msa0829520:five_prime_UTR 30.0%
TATTATACTCCAGCTGATTA+TGG - chr5_4:2517958-2517977 Msa0829520:intron 30.0%
AATCCCTAGTAGTATTAGTA+AGG - chr5_4:2518060-2518079 Msa0829520:intron 30.0%
ACTCCTTACTAATACTACTA+GGG + chr5_4:2518066-2518085 None:intergenic 30.0%
TACTCCTTACTAATACTACT+AGG + chr5_4:2518067-2518086 None:intergenic 30.0%
AACTTATCTCTAGTAGTTTG+CGG - chr5_4:2518097-2518116 Msa0829520:five_prime_UTR 30.0%
AGTAAATAACAGTTAAGAGC+AGG + chr5_4:2518209-2518228 None:intergenic 30.0%
!! TGAAAGAAAGAATAATGGGC+CGG - chr5_4:2516738-2516757 Msa0829520:three_prime_UTR 35.0%
TACAAATTATTTACCCGGGT+CGG + chr5_4:2516803-2516822 None:intergenic 35.0%
ACAACATCATGTGAGTATGA+GGG + chr5_4:2517288-2517307 None:intergenic 35.0%
AACAACATCATGTGAGTATG+AGG + chr5_4:2517289-2517308 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG + chr5_4:2517512-2517531 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG - chr5_4:2517543-2517562 Msa0829520:five_prime_UTR 35.0%
!! CTTGATCTTGATAATGACGT+GGG - chr5_4:2517544-2517563 Msa0829520:five_prime_UTR 35.0%
! TCAAAGCTTTGACAACAGTA+TGG - chr5_4:2517602-2517621 Msa0829520:five_prime_UTR 35.0%
! CAAAGCTTTGACAACAGTAT+GGG - chr5_4:2517603-2517622 Msa0829520:five_prime_UTR 35.0%
! AAAGCTTTGACAACAGTATG+GGG - chr5_4:2517604-2517623 Msa0829520:five_prime_UTR 35.0%
! ACGCACTCTTAACATTTTCA+AGG + chr5_4:2517669-2517688 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG - chr5_4:2517980-2517999 Msa0829520:intron 35.0%
!! TTGATGGAACTACTTTTCTG+AGG - chr5_4:2517996-2518015 Msa0829520:intron 35.0%
!! AACTACTTTTCTGAGGATGA+TGG - chr5_4:2518003-2518022 Msa0829520:intron 35.0%
!! TGAAAGAAAGAATAATGGGC+CGG - chr5_4:2516738-2516757 Msa0829520:three_prime_UTR 35.0%
TACAAATTATTTACCCGGGT+CGG + chr5_4:2516803-2516822 None:intergenic 35.0%
ACAACATCATGTGAGTATGA+GGG + chr5_4:2517288-2517307 None:intergenic 35.0%
AACAACATCATGTGAGTATG+AGG + chr5_4:2517289-2517308 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG + chr5_4:2517512-2517531 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG - chr5_4:2517543-2517562 Msa0829520:five_prime_UTR 35.0%
!! CTTGATCTTGATAATGACGT+GGG - chr5_4:2517544-2517563 Msa0829520:five_prime_UTR 35.0%
! TCAAAGCTTTGACAACAGTA+TGG - chr5_4:2517602-2517621 Msa0829520:five_prime_UTR 35.0%
! CAAAGCTTTGACAACAGTAT+GGG - chr5_4:2517603-2517622 Msa0829520:five_prime_UTR 35.0%
! AAAGCTTTGACAACAGTATG+GGG - chr5_4:2517604-2517623 Msa0829520:five_prime_UTR 35.0%
! ACGCACTCTTAACATTTTCA+AGG + chr5_4:2517669-2517688 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG - chr5_4:2517980-2517999 Msa0829520:intron 35.0%
!! TTGATGGAACTACTTTTCTG+AGG - chr5_4:2517996-2518015 Msa0829520:intron 35.0%
!! AACTACTTTTCTGAGGATGA+TGG - chr5_4:2518003-2518022 Msa0829520:intron 35.0%
TGTCCACCACAATTCTCTTT+CGG + chr5_4:2516777-2516796 None:intergenic 40.0%
AAGCAAGCAACCTATTCTCT+TGG + chr5_4:2516897-2516916 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG - chr5_4:2517022-2517041 Msa0829520:CDS 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr5_4:2517314-2517333 None:intergenic 40.0%
! TTAGACTTTTCCCGAACGTT+TGG - chr5_4:2517426-2517445 Msa0829520:CDS 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG - chr5_4:2517675-2517694 Msa0829520:five_prime_UTR 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG - chr5_4:2517676-2517695 Msa0829520:five_prime_UTR 40.0%
GTACTCAAATCGGTCTTCAT+AGG + chr5_4:2517860-2517879 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG - chr5_4:2517860-2517879 Msa0829520:five_prime_UTR 40.0%
!! TGAAGACCGATTTGAGTACT+GGG - chr5_4:2517861-2517880 Msa0829520:five_prime_UTR 40.0%
AATAACTAGCTGGACGTTGT+GGG + chr5_4:2517942-2517961 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG + chr5_4:2517943-2517962 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG + chr5_4:2517952-2517971 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG - chr5_4:2517966-2517985 Msa0829520:intron 40.0%
TCCTCTAATCCATAATCAGC+TGG + chr5_4:2517970-2517989 None:intergenic 40.0%
! CTTTTCTCAAGTTTGCGCTT+TGG - chr5_4:2518031-2518050 Msa0829520:intron 40.0%
TGTCCACCACAATTCTCTTT+CGG + chr5_4:2516777-2516796 None:intergenic 40.0%
AAGCAAGCAACCTATTCTCT+TGG + chr5_4:2516897-2516916 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG - chr5_4:2517022-2517041 Msa0829520:CDS 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr5_4:2517314-2517333 None:intergenic 40.0%
! TTAGACTTTTCCCGAACGTT+TGG - chr5_4:2517426-2517445 Msa0829520:CDS 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG - chr5_4:2517675-2517694 Msa0829520:five_prime_UTR 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG - chr5_4:2517676-2517695 Msa0829520:five_prime_UTR 40.0%
GTACTCAAATCGGTCTTCAT+AGG + chr5_4:2517860-2517879 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG - chr5_4:2517860-2517879 Msa0829520:five_prime_UTR 40.0%
!! TGAAGACCGATTTGAGTACT+GGG - chr5_4:2517861-2517880 Msa0829520:five_prime_UTR 40.0%
AATAACTAGCTGGACGTTGT+GGG + chr5_4:2517942-2517961 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG + chr5_4:2517943-2517962 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG + chr5_4:2517952-2517971 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG - chr5_4:2517966-2517985 Msa0829520:intron 40.0%
TCCTCTAATCCATAATCAGC+TGG + chr5_4:2517970-2517989 None:intergenic 40.0%
! CTTTTCTCAAGTTTGCGCTT+TGG - chr5_4:2518031-2518050 Msa0829520:intron 40.0%
! TAATGGGCCGGACAATGATT+TGG - chr5_4:2516750-2516769 Msa0829520:three_prime_UTR 45.0%
AATGGGCCGGACAATGATTT+GGG - chr5_4:2516751-2516770 Msa0829520:three_prime_UTR 45.0%
TTTCGGCCCAAATCATTGTC+CGG + chr5_4:2516760-2516779 None:intergenic 45.0%
TTTGGGCCGAAAGAGAATTG+TGG - chr5_4:2516768-2516787 Msa0829520:three_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG + chr5_4:2517440-2517459 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG + chr5_4:2517804-2517823 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG + chr5_4:2517805-2517824 None:intergenic 45.0%
GACTACCTTCCAACTCAGTA+TGG - chr5_4:2517823-2517842 Msa0829520:five_prime_UTR 45.0%
ACCTTCCAACTCAGTATGGT+AGG - chr5_4:2517827-2517846 Msa0829520:five_prime_UTR 45.0%
TTGAGCCTACCATACTGAGT+TGG + chr5_4:2517835-2517854 None:intergenic 45.0%
AACTCAGTATGGTAGGCTCA+AGG - chr5_4:2517834-2517853 Msa0829520:five_prime_UTR 45.0%
AGCGATCCCAGTACTCAAAT+CGG + chr5_4:2517870-2517889 None:intergenic 45.0%
! TCTCTAGTAGTTTGCGGCTT+TGG - chr5_4:2518103-2518122 Msa0829520:five_prime_UTR 45.0%
! CTCTAGTAGTTTGCGGCTTT+GGG - chr5_4:2518104-2518123 Msa0829520:five_prime_UTR 45.0%
! TAATGGGCCGGACAATGATT+TGG - chr5_4:2516750-2516769 Msa0829520:three_prime_UTR 45.0%
AATGGGCCGGACAATGATTT+GGG - chr5_4:2516751-2516770 Msa0829520:three_prime_UTR 45.0%
TTTCGGCCCAAATCATTGTC+CGG + chr5_4:2516760-2516779 None:intergenic 45.0%
TTTGGGCCGAAAGAGAATTG+TGG - chr5_4:2516768-2516787 Msa0829520:three_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG + chr5_4:2517440-2517459 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG + chr5_4:2517804-2517823 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG + chr5_4:2517805-2517824 None:intergenic 45.0%
GACTACCTTCCAACTCAGTA+TGG - chr5_4:2517823-2517842 Msa0829520:five_prime_UTR 45.0%
ACCTTCCAACTCAGTATGGT+AGG - chr5_4:2517827-2517846 Msa0829520:five_prime_UTR 45.0%
TTGAGCCTACCATACTGAGT+TGG + chr5_4:2517835-2517854 None:intergenic 45.0%
AACTCAGTATGGTAGGCTCA+AGG - chr5_4:2517834-2517853 Msa0829520:five_prime_UTR 45.0%
AGCGATCCCAGTACTCAAAT+CGG + chr5_4:2517870-2517889 None:intergenic 45.0%
! TCTCTAGTAGTTTGCGGCTT+TGG - chr5_4:2518103-2518122 Msa0829520:five_prime_UTR 45.0%
! CTCTAGTAGTTTGCGGCTTT+GGG - chr5_4:2518104-2518123 Msa0829520:five_prime_UTR 45.0%
GTATTTATAGAGCGGCCGCT+TGG + chr5_4:2516827-2516846 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG - chr5_4:2517036-2517055 Msa0829520:CDS 50.0%
AACCTCGTAACTGTTGCCAC+TGG + chr5_4:2517041-2517060 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG + chr5_4:2517439-2517458 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG - chr5_4:2517679-2517698 Msa0829520:five_prime_UTR 50.0%
CCCTGGTGATATCATGTTCG+AGG - chr5_4:2517801-2517820 Msa0829520:five_prime_UTR 50.0%
! GCCTACCATACTGAGTTGGA+AGG + chr5_4:2517831-2517850 None:intergenic 50.0%
ACAACAACAATTGGCACGCG+TGG + chr5_4:2517896-2517915 None:intergenic 50.0%
! CAATTGTTGTTGTGCGCCCA+GGG - chr5_4:2517903-2517922 Msa0829520:five_prime_UTR 50.0%
GTATTTATAGAGCGGCCGCT+TGG + chr5_4:2516827-2516846 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG - chr5_4:2517036-2517055 Msa0829520:CDS 50.0%
AACCTCGTAACTGTTGCCAC+TGG + chr5_4:2517041-2517060 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG + chr5_4:2517439-2517458 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG - chr5_4:2517679-2517698 Msa0829520:five_prime_UTR 50.0%
CCCTGGTGATATCATGTTCG+AGG - chr5_4:2517801-2517820 Msa0829520:five_prime_UTR 50.0%
! GCCTACCATACTGAGTTGGA+AGG + chr5_4:2517831-2517850 None:intergenic 50.0%
ACAACAACAATTGGCACGCG+TGG + chr5_4:2517896-2517915 None:intergenic 50.0%
! CAATTGTTGTTGTGCGCCCA+GGG - chr5_4:2517903-2517922 Msa0829520:five_prime_UTR 50.0%
GGGCCGAAAGAGAATTGTGG+TGG - chr5_4:2516771-2516790 Msa0829520:three_prime_UTR 55.0%
!! TGTGGTGGACATAACCGACC+CGG - chr5_4:2516786-2516805 Msa0829520:three_prime_UTR 55.0%
CCTGGGCGCACAACAACAAT+TGG + chr5_4:2517905-2517924 None:intergenic 55.0%
! CCAATTGTTGTTGTGCGCCC+AGG - chr5_4:2517902-2517921 Msa0829520:five_prime_UTR 55.0%
GGGCCGAAAGAGAATTGTGG+TGG - chr5_4:2516771-2516790 Msa0829520:three_prime_UTR 55.0%
!! TGTGGTGGACATAACCGACC+CGG - chr5_4:2516786-2516805 Msa0829520:three_prime_UTR 55.0%
CCTGGGCGCACAACAACAAT+TGG + chr5_4:2517905-2517924 None:intergenic 55.0%
! CCAATTGTTGTTGTGCGCCC+AGG - chr5_4:2517902-2517921 Msa0829520:five_prime_UTR 55.0%
!! GTGGTGGACATAACCGACCC+GGG - chr5_4:2516787-2516806 Msa0829520:three_prime_UTR 60.0%
!! GTGGTGGACATAACCGACCC+GGG - chr5_4:2516787-2516806 Msa0829520:three_prime_UTR 60.0%
GCTGGACGTTGTGGGCCAAG+AGG + chr5_4:2517934-2517953 None:intergenic 65.0%
GCTGGACGTTGTGGGCCAAG+AGG + chr5_4:2517934-2517953 None:intergenic 65.0%
GGGCCAAGAGGCTGCACCCT+GGG + chr5_4:2517922-2517941 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG + chr5_4:2517923-2517942 None:intergenic 70.0%
GGGCCAAGAGGCTGCACCCT+GGG + chr5_4:2517922-2517941 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG + chr5_4:2517923-2517942 None:intergenic 70.0%
GCGCCCAGGGTGCAGCCTCT+TGG - chr5_4:2517916-2517935 Msa0829520:five_prime_UTR 75.0%
GCGCCCAGGGTGCAGCCTCT+TGG - chr5_4:2517916-2517935 Msa0829520:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 2516723 2518249 2516723 ID=Msa0829520;Name=Msa0829520
chr5_4 mRNA 2516723 2518249 2516723 ID=Msa0829520-mRNA-1;Parent=Msa0829520;Name=Msa0829520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=661|1|1|1|0.5|0.33|3|250|142
chr5_4 exon 2516723 2517174 2516723 ID=Msa0829520-mRNA-1:exon:885;Parent=Msa0829520-mRNA-1
chr5_4 exon 2517253 2517957 2517253 ID=Msa0829520-mRNA-1:exon:884;Parent=Msa0829520-mRNA-1
chr5_4 exon 2518067 2518249 2518067 ID=Msa0829520-mRNA-1:exon:883;Parent=Msa0829520-mRNA-1
chr5_4 five_prime_UTR 2518067 2518249 2518067 ID=Msa0829520-mRNA-1:five_prime_utr;Parent=Msa0829520-mRNA-1
chr5_4 five_prime_UTR 2517480 2517957 2517480 ID=Msa0829520-mRNA-1:five_prime_utr;Parent=Msa0829520-mRNA-1
chr5_4 CDS 2517253 2517479 2517253 ID=Msa0829520-mRNA-1:cds;Parent=Msa0829520-mRNA-1
chr5_4 CDS 2516973 2517174 2516973 ID=Msa0829520-mRNA-1:cds;Parent=Msa0829520-mRNA-1
chr5_4 three_prime_UTR 2516723 2516972 2516723 ID=Msa0829520-mRNA-1:three_prime_utr;Parent=Msa0829520-mRNA-1
Gene Sequence

>Msa0829520

ATGATCGATAATATGTCCAATGAAAAGGCTCGTCATATTTTTAATCTTGATCTTGATCTTGATAATGACGTGGGTTTTCATTTTATAAGAAAATGTTACAAGAAGATTATCAAAGCTTTGACAACAGTATGGGGTGCTCATATTTCAAAACATGAATCAGACTTGGATACACACCTTGAAAATGTTAAGAGTGCGTATGAGAAGGGCGGCCGTGATTTTAGTGAGATTGACCCTGGTGATATCATGTTCGAGGACTACCTTCCAACTCAGTATGGTAGGCTCAAGGCCTATGAAGACCGATTTGAGTACTGGGATCGCTTGACCACGCGTGCCAATTGTTGTTGTGCGCCCAGGGTGCAGCCTCTTGGCCCACAACGTCCAGCTAGTTATTATACTCCAGCTGATTATGGATTAGAGGATGATGATTGA
Protein sequence

>Msa0829520

MIDNMSNEKARHIFNLDLDLDNDVGFHFIRKCYKKIIKALTTVWGAHISKHESDLDTHLENVKSAYEKGGRDFSEIDPGDIMFEDYLPTQYGRLKAYEDRFEYWDRLTTRANCCCAPRVQPLGPQRPASYYTPADYGLEDDD*