AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0094630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATAAATACATGATTTC+TGG 0.146830 1_2:-77952878 Msa0094630:five_prime_UTR
TATTATACTCCAGCTGATTA+TGG 0.249358 1_2:-77951755 Msa0094630:CDS
TCAAAGCTTTGACAACAGTA+TGG 0.296747 1_2:-77952111 Msa0094630:CDS
CTTTCTCTCGCCAAACGTTC+GGG 0.317643 1_2:+77952277 None:intergenic
TAATGGGCCGGACAATGATT+TGG 0.326894 1_2:-77952963 Msa0094630:five_prime_UTR
AAAACAATGGAGAGAGAAAA+AGG 0.360858 1_2:+77952392 None:intergenic
TCTTTCTCTCGCCAAACGTT+CGG 0.363000 1_2:+77952276 None:intergenic
GAAGGTGAAAGAAAGAATAA+TGG 0.363979 1_2:-77952980 Msa0094630:five_prime_UTR
TGTCCACCACAATTCTCTTT+CGG 0.378035 1_2:+77952939 None:intergenic
TCCAGCTGATTATGGATTAG+AGG 0.379886 1_2:-77951747 Msa0094630:CDS
CCTGGGCGCACAACAACAAT+TGG 0.389088 1_2:+77951811 None:intergenic
GCGCCCAGGGTGCAGCCTCT+TGG 0.396019 1_2:-77951797 Msa0094630:CDS
GTACTCAAATCGGTCTTCAT+AGG 0.417050 1_2:+77951856 None:intergenic
CCAATTGTTGTTGTGCGCCC+AGG 0.417668 1_2:-77951811 Msa0094630:CDS
AAAACAAAGTTGTCATCAAA+TGG 0.421430 1_2:-77952350 Msa0094630:five_prime_UTR
AATGGGCCGGACAATGATTT+GGG 0.427175 1_2:-77952962 Msa0094630:five_prime_UTR
GGGCCAAGAGGCTGCACCCT+GGG 0.431419 1_2:+77951794 None:intergenic
AAGGTGAAAGAAAGAATAAT+GGG 0.433797 1_2:-77952979 Msa0094630:five_prime_UTR
AACAACATCATGTGAGTATG+AGG 0.438895 1_2:+77952427 None:intergenic
ATGAAGACCGATTTGAGTAC+TGG 0.440283 1_2:-77951853 Msa0094630:CDS
GACTACCTTCCAACTCAGTA+TGG 0.445544 1_2:-77951890 Msa0094630:CDS
TCTATAAATACATGATTTCT+GGG 0.446078 1_2:-77952877 Msa0094630:five_prime_UTR
CGATAATATGTCCAATGAAA+AGG 0.453370 1_2:-77952215 Msa0094630:CDS
TCCTCTAATCCATAATCAGC+TGG 0.484339 1_2:+77951746 None:intergenic
TTTCGGCCCAAATCATTGTC+CGG 0.489949 1_2:+77952956 None:intergenic
AGCGATCCCAGTACTCAAAT+CGG 0.492770 1_2:+77951846 None:intergenic
CAAAGCTTTGACAACAGTAT+GGG 0.494576 1_2:-77952110 Msa0094630:CDS
AAGCAAGCAACCTATTCTCT+TGG 0.499131 1_2:+77952819 None:intergenic
CAATTGTTGTTGTGCGCCCA+GGG 0.500706 1_2:-77951810 Msa0094630:CDS
CCCTGGTGATATCATGTTCG+AGG 0.505379 1_2:-77951912 Msa0094630:CDS
GATTAGAGGATGATGATTGA+TGG 0.509050 1_2:-77951733 Msa0094630:exon
TACAAATTATTTACCCGGGT+CGG 0.521216 1_2:+77952913 None:intergenic
TAATAACTAGCTGGACGTTG+TGG 0.523669 1_2:+77951773 None:intergenic
AATAACTAGCTGGACGTTGT+GGG 0.528802 1_2:+77951774 None:intergenic
TTCAAAACATGAATCAGACT+TGG 0.535772 1_2:-77952077 Msa0094630:CDS
TGGGCCAAGAGGCTGCACCC+TGG 0.540694 1_2:+77951793 None:intergenic
TCCTCGAACATGATATCACC+AGG 0.543019 1_2:+77951911 None:intergenic
GCTGGAGTATAATAACTAGC+TGG 0.548205 1_2:+77951764 None:intergenic
GTATTTATAGAGCGGCCGCT+TGG 0.554556 1_2:+77952889 None:intergenic
GTTAAGAGTGCGTATGAGAA+GGG 0.563631 1_2:-77952037 Msa0094630:CDS
TGTTAAGAGTGCGTATGAGA+AGG 0.565399 1_2:-77952038 Msa0094630:CDS
TTGGTTACAAATTATTTACC+CGG 0.577076 1_2:+77952908 None:intergenic
TTGAGCCTACCATACTGAGT+TGG 0.578647 1_2:+77951881 None:intergenic
ACAACAACAATTGGCACGCG+TGG 0.596401 1_2:+77951820 None:intergenic
TGAAAGAAAGAATAATGGGC+CGG 0.596944 1_2:-77952975 Msa0094630:five_prime_UTR
AACCTCGTAACTGTTGCCAC+TGG 0.597504 1_2:+77952675 None:intergenic
TGAAGACCGATTTGAGTACT+GGG 0.608071 1_2:-77951852 Msa0094630:CDS
AACTCAGTATGGTAGGCTCA+AGG 0.609591 1_2:-77951879 Msa0094630:CDS
CTTACATCTCACTAAAATCA+CGG 0.616551 1_2:+77952011 None:intergenic
TGTGGTGGACATAACCGACC+CGG 0.621203 1_2:-77952927 Msa0094630:five_prime_UTR
CTTGATCTTGATAATGACGT+GGG 0.622967 1_2:-77952169 Msa0094630:CDS
TGAGTAATGAAAATGAAAGA+AGG 0.630657 1_2:-77952998 None:intergenic
TTCCAGTGGCAACAGTTACG+AGG 0.632420 1_2:-77952677 Msa0094630:five_prime_UTR
GGGCCGAAAGAGAATTGTGG+TGG 0.634470 1_2:-77952942 Msa0094630:five_prime_UTR
ACCTTCCAACTCAGTATGGT+AGG 0.634786 1_2:-77951886 Msa0094630:CDS
GAAATCATGTATTTATAGAG+CGG 0.640417 1_2:+77952881 None:intergenic
AAAGCTTTGACAACAGTATG+GGG 0.642690 1_2:-77952109 Msa0094630:CDS
ACAACATCATGTGAGTATGA+GGG 0.648593 1_2:+77952428 None:intergenic
TGGTTACAAATTATTTACCC+GGG 0.649137 1_2:+77952909 None:intergenic
TCTTGATCTTGATAATGACG+TGG 0.649200 1_2:-77952170 Msa0094630:CDS
GTGGTGGACATAACCGACCC+GGG 0.650786 1_2:-77952926 Msa0094630:five_prime_UTR
GCCTACCATACTGAGTTGGA+AGG 0.655535 1_2:+77951885 None:intergenic
GCTGGACGTTGTGGGCCAAG+AGG 0.661359 1_2:+77951782 None:intergenic
GTAAATAATTTGTAACCAAG+CGG 0.664321 1_2:-77952904 Msa0094630:five_prime_UTR
AAGAGTGCGTATGAGAAGGG+CGG 0.697112 1_2:-77952034 Msa0094630:CDS
CTTCACATAATGTCTTCCAG+TGG 0.702452 1_2:-77952691 Msa0094630:five_prime_UTR
TTTGGGCCGAAAGAGAATTG+TGG 0.711693 1_2:-77952945 Msa0094630:five_prime_UTR
CCTCGAACATGATATCACCA+GGG 0.756060 1_2:+77951912 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGATAATAAAACATTAA+CGG + chr1_2:77952687-77952706 None:intergenic 10.0%
!! ATTAGATAATAAAACATTAA+CGG + chr1_2:77952687-77952706 None:intergenic 10.0%
!! ACATAGTATATTAGATAAAA+GGG + chr1_2:77951893-77951912 None:intergenic 15.0%
!! AACATAGTATATTAGATAAA+AGG + chr1_2:77951894-77951913 None:intergenic 15.0%
!!! AATCACTTTTTGATAAATTT+TGG - chr1_2:77952181-77952200 Msa0094630:CDS 15.0%
!! ACATAGTATATTAGATAAAA+GGG + chr1_2:77951893-77951912 None:intergenic 15.0%
!! AACATAGTATATTAGATAAA+AGG + chr1_2:77951894-77951913 None:intergenic 15.0%
!!! AATCACTTTTTGATAAATTT+TGG - chr1_2:77952181-77952200 Msa0094630:CDS 15.0%
!! TCTATAAATACATGATTTCT+GGG - chr1_2:77951765-77951784 Msa0094630:CDS 20.0%
!! TCTATAAATACATGATTTCT+GGG - chr1_2:77951765-77951784 Msa0094630:CDS 20.0%
! AAGGTGAAAGAAAGAATAAT+GGG - chr1_2:77951663-77951682 Msa0094630:three_prime_UTR 25.0%
! TTGGTTACAAATTATTTACC+CGG + chr1_2:77951737-77951756 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG - chr1_2:77951738-77951757 Msa0094630:CDS 25.0%
! GAAATCATGTATTTATAGAG+CGG + chr1_2:77951764-77951783 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG - chr1_2:77951764-77951783 Msa0094630:CDS 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG - chr1_2:77951813-77951832 Msa0094630:CDS 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr1_2:77952266-77952285 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr1_2:77952279-77952298 None:intergenic 25.0%
! AAAACAAAGTTGTCATCAAA+TGG - chr1_2:77952292-77952311 Msa0094630:five_prime_UTR 25.0%
! AAGGTGAAAGAAAGAATAAT+GGG - chr1_2:77951663-77951682 Msa0094630:three_prime_UTR 25.0%
! TTGGTTACAAATTATTTACC+CGG + chr1_2:77951737-77951756 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG - chr1_2:77951738-77951757 Msa0094630:CDS 25.0%
! GAAATCATGTATTTATAGAG+CGG + chr1_2:77951764-77951783 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG - chr1_2:77951764-77951783 Msa0094630:CDS 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG - chr1_2:77951813-77951832 Msa0094630:CDS 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr1_2:77952266-77952285 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr1_2:77952279-77952298 None:intergenic 25.0%
! AAAACAAAGTTGTCATCAAA+TGG - chr1_2:77952292-77952311 Msa0094630:five_prime_UTR 25.0%
GAAGGTGAAAGAAAGAATAA+TGG - chr1_2:77951662-77951681 Msa0094630:three_prime_UTR 30.0%
TGGTTACAAATTATTTACCC+GGG + chr1_2:77951736-77951755 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr1_2:77952253-77952272 None:intergenic 30.0%
CGATAATATGTCCAATGAAA+AGG - chr1_2:77952427-77952446 Msa0094630:five_prime_UTR 30.0%
TTCAAAACATGAATCAGACT+TGG - chr1_2:77952565-77952584 Msa0094630:five_prime_UTR 30.0%
CTTACATCTCACTAAAATCA+CGG + chr1_2:77952634-77952653 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG + chr1_2:77952714-77952733 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG - chr1_2:77952713-77952732 Msa0094630:intron 30.0%
TATTATACTCCAGCTGATTA+TGG - chr1_2:77952887-77952906 Msa0094630:five_prime_UTR 30.0%
AATCCCTAGTAGTATTAGTA+AGG - chr1_2:77952989-77953008 Msa0094630:five_prime_UTR 30.0%
GAAGGTGAAAGAAAGAATAA+TGG - chr1_2:77951662-77951681 Msa0094630:three_prime_UTR 30.0%
TGGTTACAAATTATTTACCC+GGG + chr1_2:77951736-77951755 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr1_2:77952253-77952272 None:intergenic 30.0%
CGATAATATGTCCAATGAAA+AGG - chr1_2:77952427-77952446 Msa0094630:five_prime_UTR 30.0%
TTCAAAACATGAATCAGACT+TGG - chr1_2:77952565-77952584 Msa0094630:five_prime_UTR 30.0%
CTTACATCTCACTAAAATCA+CGG + chr1_2:77952634-77952653 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG + chr1_2:77952714-77952733 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG - chr1_2:77952713-77952732 Msa0094630:intron 30.0%
TATTATACTCCAGCTGATTA+TGG - chr1_2:77952887-77952906 Msa0094630:five_prime_UTR 30.0%
AATCCCTAGTAGTATTAGTA+AGG - chr1_2:77952989-77953008 Msa0094630:five_prime_UTR 30.0%
!! TGAAAGAAAGAATAATGGGC+CGG - chr1_2:77951667-77951686 Msa0094630:three_prime_UTR 35.0%
TACAAATTATTTACCCGGGT+CGG + chr1_2:77951732-77951751 None:intergenic 35.0%
ACAACATCATGTGAGTATGA+GGG + chr1_2:77952217-77952236 None:intergenic 35.0%
AACAACATCATGTGAGTATG+AGG + chr1_2:77952218-77952237 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG + chr1_2:77952441-77952460 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG - chr1_2:77952472-77952491 Msa0094630:five_prime_UTR 35.0%
!! CTTGATCTTGATAATGACGT+GGG - chr1_2:77952473-77952492 Msa0094630:five_prime_UTR 35.0%
! TCAAAGCTTTGACAACAGTA+TGG - chr1_2:77952531-77952550 Msa0094630:five_prime_UTR 35.0%
! CAAAGCTTTGACAACAGTAT+GGG - chr1_2:77952532-77952551 Msa0094630:five_prime_UTR 35.0%
! AAAGCTTTGACAACAGTATG+GGG - chr1_2:77952533-77952552 Msa0094630:five_prime_UTR 35.0%
! ACGCACTCTTAACATTTTCA+AGG + chr1_2:77952598-77952617 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG - chr1_2:77952909-77952928 Msa0094630:five_prime_UTR 35.0%
!! TTGATGGAACTACTTTTCTG+AGG - chr1_2:77952925-77952944 Msa0094630:five_prime_UTR 35.0%
!! AACTACTTTTCTGAGGATGA+TGG - chr1_2:77952932-77952951 Msa0094630:five_prime_UTR 35.0%
!! TGAAAGAAAGAATAATGGGC+CGG - chr1_2:77951667-77951686 Msa0094630:three_prime_UTR 35.0%
TACAAATTATTTACCCGGGT+CGG + chr1_2:77951732-77951751 None:intergenic 35.0%
ACAACATCATGTGAGTATGA+GGG + chr1_2:77952217-77952236 None:intergenic 35.0%
AACAACATCATGTGAGTATG+AGG + chr1_2:77952218-77952237 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG + chr1_2:77952441-77952460 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG - chr1_2:77952472-77952491 Msa0094630:five_prime_UTR 35.0%
!! CTTGATCTTGATAATGACGT+GGG - chr1_2:77952473-77952492 Msa0094630:five_prime_UTR 35.0%
! TCAAAGCTTTGACAACAGTA+TGG - chr1_2:77952531-77952550 Msa0094630:five_prime_UTR 35.0%
! CAAAGCTTTGACAACAGTAT+GGG - chr1_2:77952532-77952551 Msa0094630:five_prime_UTR 35.0%
! AAAGCTTTGACAACAGTATG+GGG - chr1_2:77952533-77952552 Msa0094630:five_prime_UTR 35.0%
! ACGCACTCTTAACATTTTCA+AGG + chr1_2:77952598-77952617 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG - chr1_2:77952909-77952928 Msa0094630:five_prime_UTR 35.0%
!! TTGATGGAACTACTTTTCTG+AGG - chr1_2:77952925-77952944 Msa0094630:five_prime_UTR 35.0%
!! AACTACTTTTCTGAGGATGA+TGG - chr1_2:77952932-77952951 Msa0094630:five_prime_UTR 35.0%
TGTCCACCACAATTCTCTTT+CGG + chr1_2:77951706-77951725 None:intergenic 40.0%
AAGCAAGCAACCTATTCTCT+TGG + chr1_2:77951826-77951845 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG - chr1_2:77951951-77951970 Msa0094630:intron 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr1_2:77952243-77952262 None:intergenic 40.0%
! TTAGACTTTTCCCGAACGTT+TGG - chr1_2:77952355-77952374 Msa0094630:five_prime_UTR 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG - chr1_2:77952604-77952623 Msa0094630:five_prime_UTR 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG - chr1_2:77952605-77952624 Msa0094630:five_prime_UTR 40.0%
GTACTCAAATCGGTCTTCAT+AGG + chr1_2:77952789-77952808 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG - chr1_2:77952789-77952808 Msa0094630:intron 40.0%
!! TGAAGACCGATTTGAGTACT+GGG - chr1_2:77952790-77952809 Msa0094630:intron 40.0%
AATAACTAGCTGGACGTTGT+GGG + chr1_2:77952871-77952890 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG + chr1_2:77952872-77952891 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG + chr1_2:77952881-77952900 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG - chr1_2:77952895-77952914 Msa0094630:five_prime_UTR 40.0%
TCCTCTAATCCATAATCAGC+TGG + chr1_2:77952899-77952918 None:intergenic 40.0%
! CTTTTCTCAAGTTTGCGCTT+TGG - chr1_2:77952960-77952979 Msa0094630:five_prime_UTR 40.0%
TGTCCACCACAATTCTCTTT+CGG + chr1_2:77951706-77951725 None:intergenic 40.0%
AAGCAAGCAACCTATTCTCT+TGG + chr1_2:77951826-77951845 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG - chr1_2:77951951-77951970 Msa0094630:intron 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr1_2:77952243-77952262 None:intergenic 40.0%
! TTAGACTTTTCCCGAACGTT+TGG - chr1_2:77952355-77952374 Msa0094630:five_prime_UTR 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG - chr1_2:77952604-77952623 Msa0094630:five_prime_UTR 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG - chr1_2:77952605-77952624 Msa0094630:five_prime_UTR 40.0%
GTACTCAAATCGGTCTTCAT+AGG + chr1_2:77952789-77952808 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG - chr1_2:77952789-77952808 Msa0094630:intron 40.0%
!! TGAAGACCGATTTGAGTACT+GGG - chr1_2:77952790-77952809 Msa0094630:intron 40.0%
AATAACTAGCTGGACGTTGT+GGG + chr1_2:77952871-77952890 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG + chr1_2:77952872-77952891 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG + chr1_2:77952881-77952900 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG - chr1_2:77952895-77952914 Msa0094630:five_prime_UTR 40.0%
TCCTCTAATCCATAATCAGC+TGG + chr1_2:77952899-77952918 None:intergenic 40.0%
! CTTTTCTCAAGTTTGCGCTT+TGG - chr1_2:77952960-77952979 Msa0094630:five_prime_UTR 40.0%
! TAATGGGCCGGACAATGATT+TGG - chr1_2:77951679-77951698 Msa0094630:three_prime_UTR 45.0%
AATGGGCCGGACAATGATTT+GGG - chr1_2:77951680-77951699 Msa0094630:three_prime_UTR 45.0%
TTTCGGCCCAAATCATTGTC+CGG + chr1_2:77951689-77951708 None:intergenic 45.0%
TTTGGGCCGAAAGAGAATTG+TGG - chr1_2:77951697-77951716 Msa0094630:three_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG + chr1_2:77952369-77952388 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG + chr1_2:77952733-77952752 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG + chr1_2:77952734-77952753 None:intergenic 45.0%
GACTACCTTCCAACTCAGTA+TGG - chr1_2:77952752-77952771 Msa0094630:intron 45.0%
ACCTTCCAACTCAGTATGGT+AGG - chr1_2:77952756-77952775 Msa0094630:intron 45.0%
TTGAGCCTACCATACTGAGT+TGG + chr1_2:77952764-77952783 None:intergenic 45.0%
AACTCAGTATGGTAGGCTCA+AGG - chr1_2:77952763-77952782 Msa0094630:intron 45.0%
AGCGATCCCAGTACTCAAAT+CGG + chr1_2:77952799-77952818 None:intergenic 45.0%
! TAATGGGCCGGACAATGATT+TGG - chr1_2:77951679-77951698 Msa0094630:three_prime_UTR 45.0%
AATGGGCCGGACAATGATTT+GGG - chr1_2:77951680-77951699 Msa0094630:three_prime_UTR 45.0%
TTTCGGCCCAAATCATTGTC+CGG + chr1_2:77951689-77951708 None:intergenic 45.0%
TTTGGGCCGAAAGAGAATTG+TGG - chr1_2:77951697-77951716 Msa0094630:three_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG + chr1_2:77952369-77952388 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG + chr1_2:77952733-77952752 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG + chr1_2:77952734-77952753 None:intergenic 45.0%
GACTACCTTCCAACTCAGTA+TGG - chr1_2:77952752-77952771 Msa0094630:intron 45.0%
ACCTTCCAACTCAGTATGGT+AGG - chr1_2:77952756-77952775 Msa0094630:intron 45.0%
TTGAGCCTACCATACTGAGT+TGG + chr1_2:77952764-77952783 None:intergenic 45.0%
AACTCAGTATGGTAGGCTCA+AGG - chr1_2:77952763-77952782 Msa0094630:intron 45.0%
AGCGATCCCAGTACTCAAAT+CGG + chr1_2:77952799-77952818 None:intergenic 45.0%
GTATTTATAGAGCGGCCGCT+TGG + chr1_2:77951756-77951775 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG - chr1_2:77951965-77951984 Msa0094630:intron 50.0%
AACCTCGTAACTGTTGCCAC+TGG + chr1_2:77951970-77951989 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG + chr1_2:77952368-77952387 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG - chr1_2:77952608-77952627 Msa0094630:five_prime_UTR 50.0%
CCCTGGTGATATCATGTTCG+AGG - chr1_2:77952730-77952749 Msa0094630:intron 50.0%
! GCCTACCATACTGAGTTGGA+AGG + chr1_2:77952760-77952779 None:intergenic 50.0%
ACAACAACAATTGGCACGCG+TGG + chr1_2:77952825-77952844 None:intergenic 50.0%
! CAATTGTTGTTGTGCGCCCA+GGG - chr1_2:77952832-77952851 Msa0094630:five_prime_UTR 50.0%
GTATTTATAGAGCGGCCGCT+TGG + chr1_2:77951756-77951775 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG - chr1_2:77951965-77951984 Msa0094630:intron 50.0%
AACCTCGTAACTGTTGCCAC+TGG + chr1_2:77951970-77951989 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG + chr1_2:77952368-77952387 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG - chr1_2:77952608-77952627 Msa0094630:five_prime_UTR 50.0%
CCCTGGTGATATCATGTTCG+AGG - chr1_2:77952730-77952749 Msa0094630:intron 50.0%
! GCCTACCATACTGAGTTGGA+AGG + chr1_2:77952760-77952779 None:intergenic 50.0%
ACAACAACAATTGGCACGCG+TGG + chr1_2:77952825-77952844 None:intergenic 50.0%
! CAATTGTTGTTGTGCGCCCA+GGG - chr1_2:77952832-77952851 Msa0094630:five_prime_UTR 50.0%
GGGCCGAAAGAGAATTGTGG+TGG - chr1_2:77951700-77951719 Msa0094630:three_prime_UTR 55.0%
!! TGTGGTGGACATAACCGACC+CGG - chr1_2:77951715-77951734 Msa0094630:three_prime_UTR 55.0%
CCTGGGCGCACAACAACAAT+TGG + chr1_2:77952834-77952853 None:intergenic 55.0%
! CCAATTGTTGTTGTGCGCCC+AGG - chr1_2:77952831-77952850 Msa0094630:five_prime_UTR 55.0%
GGGCCGAAAGAGAATTGTGG+TGG - chr1_2:77951700-77951719 Msa0094630:three_prime_UTR 55.0%
!! TGTGGTGGACATAACCGACC+CGG - chr1_2:77951715-77951734 Msa0094630:three_prime_UTR 55.0%
CCTGGGCGCACAACAACAAT+TGG + chr1_2:77952834-77952853 None:intergenic 55.0%
! CCAATTGTTGTTGTGCGCCC+AGG - chr1_2:77952831-77952850 Msa0094630:five_prime_UTR 55.0%
!! GTGGTGGACATAACCGACCC+GGG - chr1_2:77951716-77951735 Msa0094630:three_prime_UTR 60.0%
!! GTGGTGGACATAACCGACCC+GGG - chr1_2:77951716-77951735 Msa0094630:three_prime_UTR 60.0%
GCTGGACGTTGTGGGCCAAG+AGG + chr1_2:77952863-77952882 None:intergenic 65.0%
GCTGGACGTTGTGGGCCAAG+AGG + chr1_2:77952863-77952882 None:intergenic 65.0%
GGGCCAAGAGGCTGCACCCT+GGG + chr1_2:77952851-77952870 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG + chr1_2:77952852-77952871 None:intergenic 70.0%
GGGCCAAGAGGCTGCACCCT+GGG + chr1_2:77952851-77952870 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG + chr1_2:77952852-77952871 None:intergenic 70.0%
GCGCCCAGGGTGCAGCCTCT+TGG - chr1_2:77952845-77952864 Msa0094630:five_prime_UTR 75.0%
GCGCCCAGGGTGCAGCCTCT+TGG - chr1_2:77952845-77952864 Msa0094630:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 77951652 77953012 77951652 ID=Msa0094630;Name=Msa0094630
chr1_2 mRNA 77951652 77953012 77951652 ID=Msa0094630-mRNA-1;Parent=Msa0094630;Name=Msa0094630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=661|1|1|1|0.5|0.33|3|84|142
chr1_2 exon 77951652 77951937 77951652 ID=Msa0094630-mRNA-1:exon:27715;Parent=Msa0094630-mRNA-1
chr1_2 exon 77952016 77952720 77952016 ID=Msa0094630-mRNA-1:exon:27714;Parent=Msa0094630-mRNA-1
chr1_2 exon 77952830 77953012 77952830 ID=Msa0094630-mRNA-1:exon:27713;Parent=Msa0094630-mRNA-1
chr1_2 five_prime_UTR 77952830 77953012 77952830 ID=Msa0094630-mRNA-1:five_prime_utr;Parent=Msa0094630-mRNA-1
chr1_2 five_prime_UTR 77952243 77952720 77952243 ID=Msa0094630-mRNA-1:five_prime_utr;Parent=Msa0094630-mRNA-1
chr1_2 CDS 77952016 77952242 77952016 ID=Msa0094630-mRNA-1:cds;Parent=Msa0094630-mRNA-1
chr1_2 CDS 77951736 77951937 77951736 ID=Msa0094630-mRNA-1:cds;Parent=Msa0094630-mRNA-1
chr1_2 three_prime_UTR 77951652 77951735 77951652 ID=Msa0094630-mRNA-1:three_prime_utr;Parent=Msa0094630-mRNA-1
Gene Sequence

>Msa0094630

ATGATCGATAATATGTCCAATGAAAAGGCTCGTCATATTTTTAATCTTGATCTTGATCTTGATAATGACGTGGGTTTTCATTTTATAAGAAAATGTTACAAGAAGATTATCAAAGCTTTGACAACAGTATGGGGTGCTCATATTTCAAAACATGAATCAGACTTGGATACACACCTTGAAAATGTTAAGAGTGCGTATGAGAAGGGCGGCCGTGATTTTAGTGAGATTGACCCTGGTGATATCATGTTCGAGGACTACCTTCCAACTCAGTATGGTAGGCTCAAGGCCTATGAAGACCGATTTGAGTACTGGGATCGCTTGACCACGCGTGCCAATTGTTGTTGTGCGCCCAGGGTGCAGCCTCTTGGCCCACAACGTCCAGCTAGTTATTATACTCCAGCTGATTATGGATTAGAGGATGATGATTGA
Protein sequence

>Msa0094630

MIDNMSNEKARHIFNLDLDLDNDVGFHFIRKCYKKIIKALTTVWGAHISKHESDLDTHLENVKSAYEKGGRDFSEIDPGDIMFEDYLPTQYGRLKAYEDRFEYWDRLTTRANCCCAPRVQPLGPQRPASYYTPADYGLEDDD*