AlfalfaGEDB | Msa0832350

M. sativa cultivar zhongmu-4 / Msa0832350

KEGG: K05402
GO: -
PFAM: PF00168.33

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0832350	XP_013453283.1	95.951	247	7	3	1	244	1	247	1.69e-164	464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0832350	B7FIF5	95.951	247	7	3	1	244	1	247	8.09e-165	464

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0832350	Msa0983050	0.827177	1.806940e-54	-8.615850e-47
Msa0832350	Msa1360110	0.816389	5.671630e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0832350	MtrunA17_Chr5g0393481	95.951	247	7	3	1	244	1	247	1.56e-168	464
Msa0832350	MtrunA17_Chr5g0393541	79.412	204	33	4	7	205	3	202	1.78e-109	316
Msa0832350	MtrunA17_Chr5g0435251	29.524	105	70	2	15	115	6	110	4.69e-12	62.4
Msa0832350	MtrunA17_Chr8g0350031	27.966	118	83	2	15	131	6	122	6.43e-12	61.6
Msa0832350	MtrunA17_Chr8g0350021	27.119	118	84	2	15	131	6	122	6.11e-11	58.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0832350	AT4G34150.1	55.612	196	71	3	7	197	4	188	1.99e-69	214

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Find 86 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTAGACAAATGCTCCA+AGG	0.629387	5_4:+5378103	None:intergenic
AAGAATTACTTACCAGCGTT+TGG	0.337334	5_4:+5378315	None:intergenic
AATGCTCCAAGGCAGAAATC+TGG	0.371519	5_4:+5378114	None:intergenic
AATTACTTACCAGCGTTTGG+AGG	0.530399	5_4:+5378318	None:intergenic
AATTAGAGTGTGGTTGTTGT+GGG	0.481190	5_4:+5378517	None:intergenic
ACAGGGTAAGATGGTGGTGG+TGG	0.529461	5_4:+5378456	None:intergenic
ACGTACCTGGTGATTCACTT+TGG	0.569600	5_4:+5378716	None:intergenic
ACTTGTTGGTGGCGTATTCA+AGG	0.471023	5_4:+5379756	None:intergenic
AGGGTATGAAGGTGGTGGAT+AGG	0.481584	5_4:+5378356	None:intergenic
ATAAGCAAGACACGTTGTTG+TGG	0.580442	5_4:-5380017	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAACTGCAAATCAAATCAA+AGG	+	chr5_4:5378221-5378240	None:intergenic	30.0%
ACTTGAACGAACAATAGTAT+CGG	+	chr5_4:5379208-5379227	None:intergenic	30.0%
CATACATGTAATGTAAGGAT+TGG	-	chr5_4:5379387-5379406	Msa0832350:CDS	30.0%
GAGATGAAAATTGAAATGGT+TGG	+	chr5_4:5379774-5379793	None:intergenic	30.0%
GCAAGAGATGAAAATTGAAA+TGG	+	chr5_4:5379778-5379797	None:intergenic	30.0%
ACAACTGCAAATCAAATCAA+AGG	+	chr5_4:5378221-5378240	None:intergenic	30.0%
ACTTGAACGAACAATAGTAT+CGG	+	chr5_4:5379208-5379227	None:intergenic	30.0%
CATACATGTAATGTAAGGAT+TGG	-	chr5_4:5379387-5379406	Msa0832350:CDS	30.0%
GAGATGAAAATTGAAATGGT+TGG	+	chr5_4:5379774-5379793	None:intergenic	30.0%
GCAAGAGATGAAAATTGAAA+TGG	+	chr5_4:5379778-5379797	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	5378043	5380033	5378043	ID=Msa0832350;Name=Msa0832350
chr5_4	mRNA	5378043	5380033	5378043	ID=Msa0832350-mRNA-1;Parent=Msa0832350;Name=Msa0832350-mRNA-1;_AED=0.11;_eAED=0.11;_QI=55\|1\|0.85\|1\|0.83\|0.85\|7\|171\|244
chr5_4	exon	5378043	5378236	5378043	ID=Msa0832350-mRNA-1:exon:2306;Parent=Msa0832350-mRNA-1
chr5_4	exon	5378328	5378622	5378328	ID=Msa0832350-mRNA-1:exon:2305;Parent=Msa0832350-mRNA-1
chr5_4	exon	5378722	5378779	5378722	ID=Msa0832350-mRNA-1:exon:2304;Parent=Msa0832350-mRNA-1
chr5_4	exon	5379387	5379458	5379387	ID=Msa0832350-mRNA-1:exon:2303;Parent=Msa0832350-mRNA-1
chr5_4	exon	5379544	5379670	5379544	ID=Msa0832350-mRNA-1:exon:2302;Parent=Msa0832350-mRNA-1
chr5_4	exon	5379735	5379839	5379735	ID=Msa0832350-mRNA-1:exon:2301;Parent=Msa0832350-mRNA-1
chr5_4	exon	5379924	5380033	5379924	ID=Msa0832350-mRNA-1:exon:2300;Parent=Msa0832350-mRNA-1
chr5_4	five_prime_UTR	5379979	5380033	5379979	ID=Msa0832350-mRNA-1:five_prime_utr;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5379924	5379978	5379924	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5379735	5379839	5379735	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5379544	5379670	5379544	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5379387	5379458	5379387	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5378722	5378779	5378722	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5378328	5378622	5378328	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	CDS	5378214	5378236	5378214	ID=Msa0832350-mRNA-1:cds;Parent=Msa0832350-mRNA-1
chr5_4	three_prime_UTR	5378043	5378213	5378043	ID=Msa0832350-mRNA-1:three_prime_utr;Parent=Msa0832350-mRNA-1

Gene Sequence

>Msa0832350

ATGATGTCTAGCGGAACGATAGCGGGGATCCAAGGGCAACCTCTGGAGGTTACTGTTGTGTGTTGCTCGAAATTGAAGGACACAGAATGGATTTCAAGACAAGATCCTTATGTTTGCCTTGAATACGCCACCAACAAGTTCCGAACCAAAACGTGCACAGATGGTGGAAAAAACCCTGTTTTTCAAGAGAAGTTCGTGTTTTCCCTTATTGAAGGCCTTCGTGAGATCAATGTTGCTGTTTGGAACTACAATACTGTCACCTCTGATGATTTTATCGGCACCGGAAAGGTTCAATTGCACAAGGTTCTCTCTCAAGGTTTTGATGACTCTGCATGGCCACTTCAAACTAAAAATGGCAGACACGCAGGTGAAGTGAAACTCATAATGCATTATGCTAATGCCAAAGTGAATCACCAGAAACCAGGCTCCTCCTCAAGCCATACTCCATACATGGCAACACCACCGGCAGTCTCGTCATATAATCAAGTTCCGTCCTACTACCCACAACAACCACACTCTAATTATCACAATCCAGCAATACCTGCTGCTGCTGCTGCTTATCCACCACCACCATCTTACCCTGTTGCTTCACACAATCCATCACCCTATCCACCACCACCATCGCCATACCCTCCTCCCCACTCTTATCCTCCATCTTCAGCCTATCCACCACCTTCATACCCTCCACCTCCAGGTTATCCTCCAAACGCTGGATTCTATCCTCCGCCATACTGA

Protein sequence

>Msa0832350

MMSSGTIAGIQGQPLEVTVVCCSKLKDTEWISRQDPYVCLEYATNKFRTKTCTDGGKNPVFQEKFVFSLIEGLREINVAVWNYNTVTSDDFIGTGKVQLHKVLSQGFDDSAWPLQTKNGRHAGEVKLIMHYANAKVNHQKPGSSSSHTPYMATPPAVSSYNQVPSYYPQQPHSNYHNPAIPAAAAAYPPPPSYPVASHNPSPYPPPPSPYPPPHSYPPSSAYPPPSYPPPPGYPPNAGFYPPPY*