AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833250 MtrunA17_Chr5g0398571 98.408 314 5 0 198 511 1 314 0.0 627
Msa0833250 MtrunA17_Chr6g0467321 39.286 476 275 5 36 497 35 510 7.03e-104 321
Msa0833250 MtrunA17_Chr5g0428251 37.204 465 277 5 44 502 56 511 7.48e-100 311
Msa0833250 MtrunA17_Chr3g0140931 37.372 487 284 6 13 486 15 493 2.62e-99 310
Msa0833250 MtrunA17_Chr5g0397991 37.179 468 287 4 20 485 44 506 7.63e-98 306
Msa0833250 MtrunA17_Chr1g0203791 37.778 495 291 7 25 506 39 529 2.60e-97 304
Msa0833250 MtrunA17_Chr6g0473701 35.714 490 308 5 24 510 109 594 1.99e-95 301
Msa0833250 MtrunA17_Chr8g0389801 36.569 443 278 2 43 485 23 462 1.82e-92 289
Msa0833250 MtrunA17_Chr4g0013031 32.829 463 308 2 41 501 64 525 7.86e-90 285
Msa0833250 MtrunA17_Chr2g0295631 32.479 468 289 3 43 489 20 481 3.09e-81 261
Msa0833250 MtrunA17_Chr1g0208791 32.759 464 306 3 22 482 21 481 8.09e-75 244
Msa0833250 MtrunA17_Chr5g0398551 92.742 124 9 0 1 124 1 124 9.00e-72 224
Msa0833250 MtrunA17_Chr6g0483381 32.906 468 307 3 21 482 1 467 2.77e-70 232
Msa0833250 MtrunA17_Chr8g0392601 32.071 449 298 5 38 483 7 451 1.12e-67 225
Msa0833250 MtrunA17_Chr6g0483361 31.702 470 318 2 27 494 9 477 1.39e-67 225
Msa0833250 MtrunA17_Chr6g0449061 32.391 460 298 5 30 483 18 470 3.28e-66 221
Msa0833250 MtrunA17_Chr1g0208821 30.189 477 325 3 9 483 10 480 1.65e-64 217
Msa0833250 MtrunA17_Chr6g0477961 32.719 434 289 2 51 482 35 467 1.74e-63 214
Msa0833250 MtrunA17_Chr2g0317131 30.285 492 317 6 17 495 10 488 2.56e-63 214
Msa0833250 MtrunA17_Chr7g0251331 29.607 483 312 7 30 501 13 478 1.61e-60 206
Msa0833250 MtrunA17_Chr2g0317151 32.127 442 295 3 38 477 28 466 1.63e-59 204
Msa0833250 MtrunA17_Chr6g0483341 28.931 477 331 5 11 483 9 481 2.79e-59 203
Msa0833250 MtrunA17_Chr2g0317161 30.435 460 309 4 38 495 26 476 2.84e-56 195
Msa0833250 MtrunA17_Chr6g0483331 29.047 451 304 7 41 483 10 452 1.43e-55 192
Msa0833250 MtrunA17_Chr6g0483351 32.982 379 251 2 102 478 1 378 9.56e-55 190
Msa0833250 MtrunA17_Chr2g0317171 29.565 460 313 4 38 495 32 482 1.10e-53 188
Msa0833250 MtrunA17_Chr2g0322671 30.248 443 301 4 44 481 53 492 9.61e-53 186
Msa0833250 MtrunA17_Chr4g0024501 29.638 442 306 3 44 483 65 503 3.88e-51 182
Msa0833250 MtrunA17_Chr1g0208891 29.754 447 301 6 43 483 24 463 8.27e-49 175
Msa0833250 MtrunA17_Chr1g0208881 30.180 444 303 4 43 483 25 464 9.90e-49 174
Msa0833250 MtrunA17_Chr7g0251321 30.424 401 244 5 102 497 1 371 1.39e-47 169
Msa0833250 MtrunA17_Chr2g0288281 28.534 382 266 2 105 483 4 381 8.13e-46 164
Msa0833250 MtrunA17_Chr6g0487091 32.899 307 202 3 187 493 97 399 4.75e-45 163
Msa0833250 MtrunA17_Chr2g0317221 28.571 434 278 8 89 511 3 415 5.32e-45 163
Msa0833250 MtrunA17_Chr5g0412771 26.639 488 334 7 12 483 15 494 2.21e-43 160
Msa0833250 MtrunA17_Chr8g0368001 28.381 451 312 6 37 483 45 488 5.94e-42 157
Msa0833250 MtrunA17_Chr6g0483161 27.649 387 256 4 101 485 2 366 1.67e-41 152
Msa0833250 MtrunA17_Chr1g0154441 28.084 381 268 3 105 483 4 380 1.69e-39 147
Msa0833250 MtrunA17_Chr5g0442331 27.549 461 324 5 40 498 43 495 3.80e-39 149
Msa0833250 MtrunA17_Chr5g0412541 26.852 432 313 2 54 484 47 476 1.88e-38 147
Msa0833250 MtrunA17_Chr1g0202761 26.439 469 335 5 38 502 39 501 6.97e-38 145
Msa0833250 MtrunA17_Chr7g0254511 27.115 461 313 6 33 483 20 467 1.29e-36 141
Msa0833250 MtrunA17_Chr6g0468521 27.406 478 316 10 31 493 20 481 1.56e-36 141
Msa0833250 MtrunA17_Chr7g0254521 26.105 475 321 7 43 504 31 488 2.78e-34 135
Msa0833250 MtrunA17_Chr6g0468511 27.119 472 317 8 31 489 20 477 3.86e-33 131
Msa0833250 MtrunA17_Chr3g0132531 27.202 511 339 11 9 501 7 502 5.05e-33 131
Msa0833250 MtrunA17_Chr7g0254531 25.899 417 284 5 102 509 1 401 1.46e-31 125
Msa0833250 MtrunA17_Chr6g0468531 26.875 480 326 8 26 494 18 483 4.51e-31 125
Msa0833250 MtrunA17_Chr5g0412571 28.052 385 274 2 101 484 39 421 6.24e-31 124
Msa0833250 MtrunA17_Chr7g0248051 25.355 493 352 6 8 490 28 514 1.52e-30 124
Msa0833250 MtrunA17_Chr6g0460541 28.424 387 266 5 101 483 4 383 5.99e-28 115
Msa0833250 MtrunA17_Chr6g0483264 33.186 226 142 4 283 502 1 223 1.27e-24 102
Msa0833250 MtrunA17_Chr7g0254501 25.857 321 219 4 186 506 13 314 4.46e-20 91.3
Msa0833250 MtrunA17_Chr3g0132541 23.554 484 307 11 8 489 8 430 2.00e-18 87.8
Msa0833250 MtrunA17_Chr6g0483221 32.903 155 98 2 327 478 3 154 3.34e-18 82.4
Msa0833250 MtrunA17_Chr8g0364751 31.579 171 117 0 312 482 1 171 3.68e-16 77.0
Msa0833250 MtrunA17_Chr6g0483261 33.520 179 110 4 330 502 6 181 4.69e-16 76.3
Msa0833250 MtrunA17_Chr8g0364761 23.273 275 207 3 40 312 50 322 1.32e-15 78.2
Msa0833250 MtrunA17_Chr5g0440661 24.931 361 199 11 105 461 4 296 7.91e-15 75.5
Msa0833250 MtrunA17_Chr6g0460531 31.414 191 124 5 43 228 24 212 2.58e-14 73.2
Msa0833250 MtrunA17_Chr6g0483241 29.655 145 100 1 101 243 2 146 4.12e-14 70.9
Msa0833250 MtrunA17_Chr6g0460521 28.358 201 144 0 283 483 4 204 3.67e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833250 AT4G22790.1 58.664 479 196 2 16 493 3 480 0.0 563
Msa0833250 AT1G71870.1 37.895 475 278 5 35 492 15 489 3.69e-99 308
Msa0833250 AT4G29140.1 34.731 501 292 8 24 500 34 523 7.82e-95 298
Msa0833250 AT1G58340.1 39.520 458 266 3 43 495 57 508 2.58e-93 294
Msa0833250 AT5G19700.1 37.310 461 284 3 36 494 28 485 5.12e-93 293
Msa0833250 AT5G52050.1 31.703 511 332 6 11 510 1 505 2.70e-90 285
Msa0833250 AT4G23030.1 36.952 479 291 5 30 502 15 488 1.25e-83 268
Msa0833250 AT2G38510.1 31.712 473 318 4 41 511 3 472 5.30e-83 266
Msa0833250 AT5G49130.1 33.970 471 298 2 35 492 17 487 5.90e-83 266
Msa0833250 AT2G04040.1 33.184 446 291 3 41 483 24 465 7.02e-68 226
Msa0833250 AT1G66760.2 30.398 477 325 3 18 491 2 474 1.19e-67 226
Msa0833250 AT5G52450.1 31.906 467 312 4 38 501 20 483 2.60e-65 219
Msa0833250 AT1G66760.1 30.388 464 316 3 18 478 2 461 6.24e-65 218
Msa0833250 AT2G04080.1 30.454 463 315 3 24 483 7 465 3.96e-63 213
Msa0833250 AT2G34360.1 29.622 476 315 8 33 501 16 478 1.40e-62 212
Msa0833250 AT1G71140.1 30.131 458 312 4 29 483 12 464 1.93e-61 209
Msa0833250 AT2G04070.1 30.670 463 314 3 24 483 7 465 2.06e-61 209
Msa0833250 AT2G04080.2 30.108 465 318 3 24 485 7 467 4.04e-61 208
Msa0833250 AT1G15160.1 30.543 442 290 4 68 502 55 486 5.44e-61 208
Msa0833250 AT1G64820.1 31.635 471 301 6 33 492 18 478 6.24e-61 208
Msa0833250 AT2G04100.1 30.562 445 304 2 41 483 27 468 7.55e-61 207
Msa0833250 AT1G15150.1 30.064 469 311 4 41 502 28 486 1.29e-60 207
Msa0833250 AT1G66780.1 32.791 430 277 4 68 494 59 479 6.09e-60 205
Msa0833250 AT1G73700.1 30.335 478 317 7 24 494 7 475 2.40e-59 203
Msa0833250 AT1G15170.1 31.991 447 289 4 44 483 34 472 4.11e-59 203
Msa0833250 AT2G04050.1 31.303 476 303 5 21 483 1 465 6.59e-59 202
Msa0833250 AT2G04100.2 30.000 440 303 2 41 478 27 463 2.84e-58 200
Msa0833250 AT2G04090.1 29.213 445 310 2 41 483 27 468 2.81e-57 198
Msa0833250 AT1G15180.1 31.707 451 293 4 40 483 31 473 5.81e-55 192
Msa0833250 AT2G34360.2 28.361 476 279 9 33 501 16 436 8.60e-55 190
Msa0833250 AT3G23550.1 30.612 441 299 5 43 480 30 466 1.36e-51 182
Msa0833250 AT1G66760.4 29.798 396 271 3 18 410 2 393 8.07e-51 178
Msa0833250 AT1G61890.1 28.228 457 311 6 44 493 47 493 1.05e-50 181
Msa0833250 AT1G11670.1 28.188 447 306 5 44 483 50 488 1.50e-50 181
Msa0833250 AT1G61890.2 28.054 442 303 5 44 478 47 480 1.54e-48 175
Msa0833250 AT1G15170.2 30.726 358 233 4 133 483 4 353 5.10e-47 167
Msa0833250 AT1G47530.1 27.872 470 326 4 19 483 11 472 1.58e-46 169
Msa0833250 AT3G26590.1 27.766 479 325 8 33 501 28 495 1.46e-45 167
Msa0833250 AT1G61890.3 28.842 423 292 5 44 462 47 464 1.52e-45 166
Msa0833250 AT3G21690.1 29.322 457 315 4 44 495 52 505 1.69e-45 167
Msa0833250 AT3G23560.1 29.186 442 308 3 38 477 33 471 1.94e-45 166
Msa0833250 AT1G15150.2 28.912 377 251 4 133 502 4 370 5.16e-45 162
Msa0833250 AT4G21910.3 26.697 442 318 4 44 483 55 492 7.73e-44 162
Msa0833250 AT4G21910.1 26.697 442 318 4 44 483 55 492 7.73e-44 162
Msa0833250 AT1G12950.1 28.330 473 305 12 43 498 62 517 2.80e-43 161
Msa0833250 AT4G21910.2 27.088 443 315 5 44 483 57 494 9.13e-43 159
Msa0833250 AT4G21910.4 27.088 443 315 5 44 483 57 494 9.13e-43 159
Msa0833250 AT5G38030.1 27.079 469 329 5 24 483 18 482 2.30e-41 155
Msa0833250 AT3G26590.2 28.321 399 275 4 105 501 4 393 6.58e-41 152
Msa0833250 AT4G00350.1 24.440 491 341 5 11 483 46 524 2.29e-40 153
Msa0833250 AT4G21903.1 26.812 414 297 4 72 483 81 490 1.07e-39 150
Msa0833250 AT5G10420.1 28.540 452 305 6 54 499 48 487 1.58e-39 150
Msa0833250 AT5G44050.1 28.918 453 307 7 46 493 46 488 6.85e-39 148
Msa0833250 AT1G23300.1 26.793 474 323 8 51 510 51 514 3.60e-38 147
Msa0833250 AT4G21903.2 26.506 415 299 4 72 484 81 491 5.06e-38 146
Msa0833250 AT3G23560.2 30.058 346 237 3 38 381 33 375 3.60e-37 142
Msa0833250 AT4G21903.3 27.034 381 273 3 105 483 4 381 5.58e-37 141
Msa0833250 AT3G59030.1 25.629 437 313 6 68 501 75 502 1.44e-36 142
Msa0833250 AT5G65380.1 27.273 462 323 6 28 483 21 475 4.93e-36 140
Msa0833250 AT1G47530.2 27.604 384 266 3 105 483 4 380 1.66e-35 137
Msa0833250 AT4G25640.1 25.630 476 331 5 21 481 1 468 2.50e-34 135
Msa0833250 AT4G25640.2 25.630 476 331 5 21 481 1 468 3.25e-34 135
Msa0833250 AT1G33100.1 27.556 450 312 6 41 482 26 469 1.02e-33 134
Msa0833250 AT1G33110.1 28.959 442 292 6 51 482 43 472 4.94e-33 132
Msa0833250 AT1G33090.1 27.434 452 310 5 41 482 29 472 6.05e-33 131
Msa0833250 AT1G66760.3 27.893 337 236 3 145 478 1 333 2.30e-32 127
Msa0833250 AT4G25640.3 25.450 444 308 5 21 449 1 436 3.45e-31 126
Msa0833250 AT3G03620.2 26.190 462 323 7 28 481 21 472 6.96e-31 125
Msa0833250 AT3G03620.1 26.190 462 323 7 28 481 21 472 6.96e-31 125
Msa0833250 AT5G17700.2 25.263 475 324 9 30 492 20 475 7.64e-31 125
Msa0833250 AT5G17700.1 25.263 475 324 9 30 492 20 475 7.64e-31 125
Msa0833250 AT1G15180.2 29.779 272 176 4 133 397 4 267 9.03e-30 121
Msa0833250 AT1G33080.2 26.126 444 310 6 51 486 43 476 8.31e-29 119
Msa0833250 AT1G33080.1 25.909 440 308 6 51 482 43 472 3.59e-28 117
Msa0833250 AT3G59030.2 25.740 338 244 5 68 402 75 408 2.41e-23 102
Msa0833250 AT1G33110.2 29.834 362 232 6 51 402 43 392 4.51e-23 101
Msa0833250 AT5G17700.3 25.455 385 261 8 30 402 20 390 7.00e-21 95.1

Find 121 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGCTGCAAGGGGAATTT+GGG 0.166621 5_4:+6447920 Msa0833250:CDS
CTCTTCTTTGGACTTCTTAC+TGG 0.194547 5_4:+6448170 Msa0833250:CDS
GGCCTTGTTGGACTCTTCTT+TGG 0.195245 5_4:+6448158 Msa0833250:CDS
TTCACTTTCGCTAACGTTAC+TGG 0.209280 5_4:+6447081 Msa0833250:CDS
TGGTGGCTGCAAGGGGAATT+TGG 0.224291 5_4:+6447919 Msa0833250:CDS
TCTTATTGTTGTTGTTCTTC+TGG 0.235360 5_4:+6447515 Msa0833250:CDS
AGCTTAGCCGGTGGCGCGCT+CGG 0.247230 5_4:+6447057 Msa0833250:CDS
ATGTCATCAACATGCAAATT+AGG 0.269692 5_4:+6446820 Msa0833250:CDS
GAAATTCTAGTCTTGCTTAC+TGG 0.273403 5_4:+6447684 Msa0833250:CDS
CTCTAGAATTAAAACATAAA+TGG 0.277546 5_4:-6447538 None:intergenic
CTTCCTACCATGTTTAGTTC+TGG 0.293099 5_4:+6447402 Msa0833250:CDS
TGAAGCCACATGAAAGTAAT+AGG 0.326554 5_4:-6447234 None:intergenic
CATGATAGGGGCACTATAAA+TGG 0.328539 5_4:+6447957 Msa0833250:CDS
AAGCTTCGTCTTGGCCTTGT+TGG 0.336381 5_4:+6448146 Msa0833250:CDS
CAAGAAATTTCCACTGTTGC+TGG 0.340485 5_4:+6447291 Msa0833250:CDS
TTTGAGAGTAGTATGTTAAC+AGG 0.351355 5_4:-6447447 None:intergenic
CCATGAGCCTGTCCACAAAT+AGG 0.351874 5_4:-6447141 None:intergenic
CACAGGGAACTTCTTTAACT+TGG 0.357675 5_4:-6448286 None:intergenic
GCTTAGCCGGTGGCGCGCTC+GGG 0.363500 5_4:+6447058 Msa0833250:CDS
AGAGTATTATGGATTGGATT+AGG 0.376619 5_4:+6447604 Msa0833250:CDS
TGCTGTTGTGATTGCTGTCT+TGG 0.380543 5_4:-6447010 None:intergenic
CAGTGGAAATTTCTTGTTGT+TGG 0.381992 5_4:-6447284 None:intergenic
TTTCTCAGTGATGCTGTCAT+TGG 0.384106 5_4:+6447773 Msa0833250:CDS
GCTAAATTCATCAACATCAT+TGG 0.390023 5_4:-6446979 None:intergenic
GAGGGGTTTCCATGGCTGTT+TGG 0.394339 5_4:+6447484 Msa0833250:CDS
ATTAGGCTAATCAAGCTATC+TGG 0.398009 5_4:+6447621 Msa0833250:CDS
GGCAACTCTTCAGCCATGAT+AGG 0.398212 5_4:+6447943 Msa0833250:CDS
GTGGCTGCAAGGGGAATTTG+GGG 0.399284 5_4:+6447921 Msa0833250:CDS
ACTGGCTTCTCTGTCTTGAA+TGG 0.406137 5_4:+6447099 Msa0833250:CDS
TCAGAGGAGCTAAGAATTCA+AGG 0.406276 5_4:+6446943 Msa0833250:CDS
GACAATTGTACTGTTGCTAT+TGG 0.410125 5_4:+6447203 Msa0833250:CDS
CGCAGGTTGCACCGGCAGCT+TGG 0.414906 5_4:+6447893 Msa0833250:CDS
GCTTGGTGATGGTGGCTGCA+AGG 0.424849 5_4:+6447910 Msa0833250:CDS
CGCAAACGCCAAGCAAGCAT+TGG 0.427319 5_4:+6447713 Msa0833250:CDS
CGCCAAGCTCGTTTGAGACA+CGG 0.430905 5_4:-6447812 None:intergenic
TGGGTTTGTAATGGTGGAGG+AGG 0.431977 5_4:-6446886 None:intergenic
CTTGGTGATGGTGGCTGCAA+GGG 0.432045 5_4:+6447911 Msa0833250:CDS
TATTATTAGTATTCATTGCT+AGG 0.432752 5_4:+6448213 Msa0833250:CDS
AGGGACAGCTCGACCTTGGT+TGG 0.445218 5_4:+6448058 Msa0833250:CDS
TGGTACTTCTGCGTCATCAC+AGG 0.449230 5_4:-6448303 None:intergenic
GCGCGTGTGTCTCTAGGAAT+AGG 0.451272 5_4:+6447864 Msa0833250:CDS
TTTGCCTTCAAGCTTCGTCT+TGG 0.451622 5_4:+6448137 Msa0833250:CDS
CATTTATAGTGCCCCTATCA+TGG 0.457544 5_4:-6447956 None:intergenic
ACCGGTACGCTCGGTCGGCT+TGG 0.459703 5_4:-6447839 None:intergenic
TAAAACTCCCAATGCTTGCT+TGG 0.462126 5_4:-6447721 None:intergenic
TCTAGAGAATCGAAAGGTAT+TGG 0.462933 5_4:+6447554 Msa0833250:CDS
ACTTGCTAATATTTGTGCCT+TGG 0.463290 5_4:-6448258 None:intergenic
CGGTCAGCGCGTGTGTCTCT+AGG 0.465994 5_4:+6447858 Msa0833250:CDS
TCTGAAAGCAGCGTCTTCGC+TGG 0.467091 5_4:-6446925 None:intergenic
TTGGGTATGTATGCAAATCT+AGG 0.477841 5_4:+6448077 Msa0833250:CDS
CGTGGAAAGAAGGAGGATGG+TGG 0.478669 5_4:+6447577 Msa0833250:CDS
ATGGTGGGATCAGAGTATTA+TGG 0.479182 5_4:+6447593 Msa0833250:CDS
CTAGGTGAGCTCAGCTTAGC+CGG 0.481845 5_4:+6447045 Msa0833250:CDS
GCAACTCTTCAGCCATGATA+GGG 0.483823 5_4:+6447944 Msa0833250:CDS
AGTGACATAACAAACAAATC+AGG 0.486224 5_4:-6447336 None:intergenic
GAATGGTCTCTGTGGTGCCA+TGG 0.486890 5_4:+6447116 Msa0833250:CDS
TTGTTGTCTCAACACATGTA+TGG 0.488430 5_4:+6447647 Msa0833250:CDS
GGGACAGCTCGACCTTGGTT+GGG 0.495004 5_4:+6448059 Msa0833250:CDS
GGGATCAGAGTATTATGGAT+TGG 0.495416 5_4:+6447598 Msa0833250:CDS
ACCCGTGTCTCAAACGAGCT+TGG 0.500843 5_4:+6447810 Msa0833250:CDS
CCTATTTGTGGACAGGCTCA+TGG 0.501376 5_4:+6447141 Msa0833250:CDS
TATTCATTGCTAGGATAAAG+TGG 0.503924 5_4:+6448222 Msa0833250:CDS
AAGGCCAAGACGAAGCTTGA+AGG 0.508427 5_4:-6448141 None:intergenic
GGGTCTAAGAGGGGTTTCCA+TGG 0.510224 5_4:+6447476 Msa0833250:CDS
GCCAAGCTCGTTTGAGACAC+GGG 0.510977 5_4:-6447811 None:intergenic
AGTGAACCCGAGCGCGCCAC+CGG 0.515331 5_4:-6447064 None:intergenic
AAGGTATTGGCGTGGAAAGA+AGG 0.518806 5_4:+6447567 Msa0833250:CDS
GCAAACGCCAAGCAAGCATT+GGG 0.519010 5_4:+6447714 Msa0833250:CDS
AGCCACCATCACCAAGCTGC+CGG 0.520002 5_4:-6447904 None:intergenic
CACGCGCTGACCGGTACGCT+CGG 0.520838 5_4:-6447848 None:intergenic
CATGGAACCTATTTGTGGAC+AGG 0.523239 5_4:+6447134 Msa0833250:CDS
TTGCACCGGCAGCTTGGTGA+TGG 0.528564 5_4:+6447899 Msa0833250:CDS
GTGGAGGAGGTGACATTGGT+TGG 0.531112 5_4:-6446873 None:intergenic
TTCGAGGGACAGCTCGACCT+TGG 0.533695 5_4:+6448054 Msa0833250:CDS
CACCGGCAGCTTGGTGATGG+TGG 0.540745 5_4:+6447902 Msa0833250:CDS
CTAGAGACACACGCGCTGAC+CGG 0.547309 5_4:-6447857 None:intergenic
AGCGAAGACGCTGCTTTCAG+AGG 0.548362 5_4:+6446927 Msa0833250:CDS
TGATGTTGATGAATTTAGCT+TGG 0.549712 5_4:+6446983 Msa0833250:CDS
GTTTGTGGAGGCATAGTTCG+AGG 0.549958 5_4:+6448038 Msa0833250:CDS
ATGCCTCCACAAACTGCCAA+TGG 0.550253 5_4:-6448029 None:intergenic
ATCAGTTATCCAAACAGCCA+TGG 0.552397 5_4:-6447493 None:intergenic
TGGCGTGGAAAGAAGGAGGA+TGG 0.552982 5_4:+6447574 Msa0833250:CDS
AATGGTGGAGGAGGTGACAT+TGG 0.553401 5_4:-6446877 None:intergenic
AATGTTGTTGATGGCTCTAG+TGG 0.559949 5_4:+6447992 Msa0833250:CDS
GTATGTATGCAAATCTAGGC+GGG 0.568375 5_4:+6448081 Msa0833250:CDS
CGCTGACCGGTACGCTCGGT+CGG 0.568556 5_4:-6447844 None:intergenic
CTTGCTTGGCGTTTGCGAGG+TGG 0.568986 5_4:-6447707 None:intergenic
GGTGCCATGGAACCTATTTG+TGG 0.570180 5_4:+6447129 Msa0833250:CDS
GTGGAAAGAAGGAGGATGGT+GGG 0.570875 5_4:+6447578 Msa0833250:CDS
CTGTCCACAAATAGGTTCCA+TGG 0.574641 5_4:-6447133 None:intergenic
CAATTCCTATTACTTTCATG+TGG 0.579908 5_4:+6447229 Msa0833250:CDS
ACCAAGCCGACCGAGCGTAC+CGG 0.581822 5_4:+6447838 Msa0833250:CDS
GGAAACCCCTCTTAGACCCA+TGG 0.585351 5_4:-6447472 None:intergenic
TTTCCATTGGCAGTTTGTGG+AGG 0.586502 5_4:+6448026 Msa0833250:CDS
TGGCTGCAAGGGGAATTTGG+GGG 0.591531 5_4:+6447922 Msa0833250:CDS
AACATAAGTACCAGCAACAG+TGG 0.598946 5_4:-6447301 None:intergenic
GTGGGTGGAAGAAGCTGCCA+AGG 0.602858 5_4:+6448241 Msa0833250:CDS
TTGGTGATGGTGGCTGCAAG+GGG 0.610161 5_4:+6447912 Msa0833250:CDS
ATGCTTGCTTGGCGTTTGCG+AGG 0.612886 5_4:-6447710 None:intergenic
ACACCAGAACTAAACATGGT+AGG 0.622764 5_4:-6447405 None:intergenic
TTTAATTCTAGAGAATCGAA+AGG 0.625448 5_4:+6447548 Msa0833250:CDS
GGTACTTCTGCGTCATCACA+GGG 0.629425 5_4:-6448302 None:intergenic
GTATTGGCGTGGAAAGAAGG+AGG 0.632332 5_4:+6447570 Msa0833250:CDS
ATTCATTGCTAGGATAAAGT+GGG 0.633439 5_4:+6448223 Msa0833250:CDS
CAACTCTTCAGCCATGATAG+GGG 0.633765 5_4:+6447945 Msa0833250:CDS
GGTGAGCTCAGCTTAGCCGG+TGG 0.638224 5_4:+6447048 Msa0833250:CDS
TTTGTGGAGGCATAGTTCGA+GGG 0.638241 5_4:+6448039 Msa0833250:CDS
GACACGGGTGGAAACACAAG+TGG 0.646609 5_4:-6447796 None:intergenic
TGCTACACCAGAACTAAACA+TGG 0.651792 5_4:-6447409 None:intergenic
GTCCAAAGAAGAGTCCAACA+AGG 0.652628 5_4:-6448160 None:intergenic
CATTGCTAGGATAAAGTGGG+TGG 0.653404 5_4:+6448226 Msa0833250:CDS
TCTGTCTTGAATGGTCTCTG+TGG 0.655881 5_4:+6447108 Msa0833250:CDS
AGAATCGAAAGGTATTGGCG+TGG 0.660271 5_4:+6447559 Msa0833250:CDS
TCAACACATGTATGGAGTGG+TGG 0.666907 5_4:+6447655 Msa0833250:CDS
TCATGAGAAGTGTCTTGTGA+AGG 0.670945 5_4:-6447182 None:intergenic
ATAGTCAAAGTTCAACACAA+TGG 0.680940 5_4:-6447745 None:intergenic
GTCTCAACACATGTATGGAG+TGG 0.690627 5_4:+6447652 Msa0833250:CDS
AAGCTCGTTTGAGACACGGG+TGG 0.702539 5_4:-6447808 None:intergenic
GGTATGTATGCAAATCTAGG+CGG 0.704766 5_4:+6448080 Msa0833250:CDS
TGACGCAGAAGTACCAACTG+AGG 0.735542 5_4:+6448310 Msa0833250:CDS
TTTGCATACATACCCAACCA+AGG 0.735927 5_4:-6448071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GACAAAATTTTGATTCATTT+TGG + chr5_4:6447261-6447280 Msa0833250:CDS 20.0%
!!! CTCTAGAATTAAAACATAAA+TGG - chr5_4:6447541-6447560 None:intergenic 20.0%
!! TATTATTAGTATTCATTGCT+AGG + chr5_4:6448213-6448232 Msa0833250:CDS 20.0%
!!! GACAAAATTTTGATTCATTT+TGG + chr5_4:6447261-6447280 Msa0833250:CDS 20.0%
!!! CTCTAGAATTAAAACATAAA+TGG - chr5_4:6447541-6447560 None:intergenic 20.0%
!! TATTATTAGTATTCATTGCT+AGG + chr5_4:6448213-6448232 Msa0833250:CDS 20.0%
!! TTTAATTCTAGAGAATCGAA+AGG + chr5_4:6447548-6447567 Msa0833250:CDS 25.0%
! AAAAAAGACAATGTTGTTGA+TGG + chr5_4:6447983-6448002 Msa0833250:CDS 25.0%
!! TTTAATTCTAGAGAATCGAA+AGG + chr5_4:6447548-6447567 Msa0833250:CDS 25.0%
! AAAAAAGACAATGTTGTTGA+TGG + chr5_4:6447983-6448002 Msa0833250:CDS 25.0%
ATGTCATCAACATGCAAATT+AGG + chr5_4:6446820-6446839 Msa0833250:CDS 30.0%
!!! TAGTTTTATGGTTTGAGCTA+AGG - chr5_4:6446852-6446871 None:intergenic 30.0%
!!! ACATTGGTTGGTTAGTTTTA+TGG - chr5_4:6446864-6446883 None:intergenic 30.0%
!!! TCACATTTTTGGGTTTGTAA+TGG - chr5_4:6446898-6446917 None:intergenic 30.0%
GCTAAATTCATCAACATCAT+TGG - chr5_4:6446982-6447001 None:intergenic 30.0%
! TGATGTTGATGAATTTAGCT+TGG + chr5_4:6446983-6447002 Msa0833250:CDS 30.0%
CAATTCCTATTACTTTCATG+TGG + chr5_4:6447229-6447248 Msa0833250:CDS 30.0%
AGTGACATAACAAACAAATC+AGG - chr5_4:6447339-6447358 None:intergenic 30.0%
TTTGAGAGTAGTATGTTAAC+AGG - chr5_4:6447450-6447469 None:intergenic 30.0%
ATACTACTCTCAAAAACCAT+GGG + chr5_4:6447456-6447475 Msa0833250:CDS 30.0%
! TCTTATTGTTGTTGTTCTTC+TGG + chr5_4:6447515-6447534 Msa0833250:CDS 30.0%
!! AGAGTATTATGGATTGGATT+AGG + chr5_4:6447604-6447623 Msa0833250:CDS 30.0%
ATAGTCAAAGTTCAACACAA+TGG - chr5_4:6447748-6447767 None:intergenic 30.0%
!! GGAGTTGTTTAATTTTCCAT+TGG + chr5_4:6448013-6448032 Msa0833250:CDS 30.0%
TATTCATTGCTAGGATAAAG+TGG + chr5_4:6448222-6448241 Msa0833250:CDS 30.0%
ATTCATTGCTAGGATAAAGT+GGG + chr5_4:6448223-6448242 Msa0833250:CDS 30.0%
ATGTCATCAACATGCAAATT+AGG + chr5_4:6446820-6446839 Msa0833250:CDS 30.0%
!!! TAGTTTTATGGTTTGAGCTA+AGG - chr5_4:6446852-6446871 None:intergenic 30.0%
!!! ACATTGGTTGGTTAGTTTTA+TGG - chr5_4:6446864-6446883 None:intergenic 30.0%
!!! TCACATTTTTGGGTTTGTAA+TGG - chr5_4:6446898-6446917 None:intergenic 30.0%
GCTAAATTCATCAACATCAT+TGG - chr5_4:6446982-6447001 None:intergenic 30.0%
! TGATGTTGATGAATTTAGCT+TGG + chr5_4:6446983-6447002 Msa0833250:CDS 30.0%
CAATTCCTATTACTTTCATG+TGG + chr5_4:6447229-6447248 Msa0833250:CDS 30.0%
AGTGACATAACAAACAAATC+AGG - chr5_4:6447339-6447358 None:intergenic 30.0%
TTTGAGAGTAGTATGTTAAC+AGG - chr5_4:6447450-6447469 None:intergenic 30.0%
ATACTACTCTCAAAAACCAT+GGG + chr5_4:6447456-6447475 Msa0833250:CDS 30.0%
! TCTTATTGTTGTTGTTCTTC+TGG + chr5_4:6447515-6447534 Msa0833250:CDS 30.0%
!! AGAGTATTATGGATTGGATT+AGG + chr5_4:6447604-6447623 Msa0833250:CDS 30.0%
ATAGTCAAAGTTCAACACAA+TGG - chr5_4:6447748-6447767 None:intergenic 30.0%
!! GGAGTTGTTTAATTTTCCAT+TGG + chr5_4:6448013-6448032 Msa0833250:CDS 30.0%
TATTCATTGCTAGGATAAAG+TGG + chr5_4:6448222-6448241 Msa0833250:CDS 30.0%
ATTCATTGCTAGGATAAAGT+GGG + chr5_4:6448223-6448242 Msa0833250:CDS 30.0%
!!! CATTTTTGGGTTTGTAATGG+TGG - chr5_4:6446895-6446914 None:intergenic 35.0%
! TGGATTTGCGTCACATTTTT+GGG - chr5_4:6446908-6446927 None:intergenic 35.0%
! AGCAATCACAACAGCATTTT+TGG + chr5_4:6447017-6447036 Msa0833250:CDS 35.0%
! GCAATCACAACAGCATTTTT+GGG + chr5_4:6447018-6447037 Msa0833250:CDS 35.0%
GACAATTGTACTGTTGCTAT+TGG + chr5_4:6447203-6447222 Msa0833250:CDS 35.0%
TGAAGCCACATGAAAGTAAT+AGG - chr5_4:6447237-6447256 None:intergenic 35.0%
! CAGTGGAAATTTCTTGTTGT+TGG - chr5_4:6447287-6447306 None:intergenic 35.0%
! GAGCTCAAGTAAGCTTTTAA+GGG - chr5_4:6447369-6447388 None:intergenic 35.0%
! AGAGCTCAAGTAAGCTTTTA+AGG - chr5_4:6447370-6447389 None:intergenic 35.0%
CATACTACTCTCAAAAACCA+TGG + chr5_4:6447455-6447474 Msa0833250:CDS 35.0%
! TCTAGAGAATCGAAAGGTAT+TGG + chr5_4:6447554-6447573 Msa0833250:CDS 35.0%
ATTAGGCTAATCAAGCTATC+TGG + chr5_4:6447621-6447640 Msa0833250:CDS 35.0%
TTGTTGTCTCAACACATGTA+TGG + chr5_4:6447647-6447666 Msa0833250:CDS 35.0%
!! GAAATTCTAGTCTTGCTTAC+TGG + chr5_4:6447684-6447703 Msa0833250:CDS 35.0%
! AATTTTCCATTGGCAGTTTG+TGG + chr5_4:6448023-6448042 Msa0833250:CDS 35.0%
TTGGGTATGTATGCAAATCT+AGG + chr5_4:6448077-6448096 Msa0833250:CDS 35.0%
! ACTTGCTAATATTTGTGCCT+TGG - chr5_4:6448261-6448280 None:intergenic 35.0%
!!! CATTTTTGGGTTTGTAATGG+TGG - chr5_4:6446895-6446914 None:intergenic 35.0%
! TGGATTTGCGTCACATTTTT+GGG - chr5_4:6446908-6446927 None:intergenic 35.0%
! AGCAATCACAACAGCATTTT+TGG + chr5_4:6447017-6447036 Msa0833250:CDS 35.0%
! GCAATCACAACAGCATTTTT+GGG + chr5_4:6447018-6447037 Msa0833250:CDS 35.0%
GACAATTGTACTGTTGCTAT+TGG + chr5_4:6447203-6447222 Msa0833250:CDS 35.0%
TGAAGCCACATGAAAGTAAT+AGG - chr5_4:6447237-6447256 None:intergenic 35.0%
! CAGTGGAAATTTCTTGTTGT+TGG - chr5_4:6447287-6447306 None:intergenic 35.0%
! GAGCTCAAGTAAGCTTTTAA+GGG - chr5_4:6447369-6447388 None:intergenic 35.0%
! AGAGCTCAAGTAAGCTTTTA+AGG - chr5_4:6447370-6447389 None:intergenic 35.0%
CATACTACTCTCAAAAACCA+TGG + chr5_4:6447455-6447474 Msa0833250:CDS 35.0%
! TCTAGAGAATCGAAAGGTAT+TGG + chr5_4:6447554-6447573 Msa0833250:CDS 35.0%
ATTAGGCTAATCAAGCTATC+TGG + chr5_4:6447621-6447640 Msa0833250:CDS 35.0%
TTGTTGTCTCAACACATGTA+TGG + chr5_4:6447647-6447666 Msa0833250:CDS 35.0%
!! GAAATTCTAGTCTTGCTTAC+TGG + chr5_4:6447684-6447703 Msa0833250:CDS 35.0%
! AATTTTCCATTGGCAGTTTG+TGG + chr5_4:6448023-6448042 Msa0833250:CDS 35.0%
TTGGGTATGTATGCAAATCT+AGG + chr5_4:6448077-6448096 Msa0833250:CDS 35.0%
! ACTTGCTAATATTTGTGCCT+TGG - chr5_4:6448261-6448280 None:intergenic 35.0%
!!! TTTTGGGTTTGTAATGGTGG+AGG - chr5_4:6446892-6446911 None:intergenic 40.0%
! CTGGATTTGCGTCACATTTT+TGG - chr5_4:6446909-6446928 None:intergenic 40.0%
TCAGAGGAGCTAAGAATTCA+AGG + chr5_4:6446943-6446962 Msa0833250:CDS 40.0%
TTCACTTTCGCTAACGTTAC+TGG + chr5_4:6447081-6447100 Msa0833250:CDS 40.0%
!! TCATGAGAAGTGTCTTGTGA+AGG - chr5_4:6447185-6447204 None:intergenic 40.0%
CAAGAAATTTCCACTGTTGC+TGG + chr5_4:6447291-6447310 Msa0833250:CDS 40.0%
AACATAAGTACCAGCAACAG+TGG - chr5_4:6447304-6447323 None:intergenic 40.0%
! CTTCCTACCATGTTTAGTTC+TGG + chr5_4:6447402-6447421 Msa0833250:CDS 40.0%
ACACCAGAACTAAACATGGT+AGG - chr5_4:6447408-6447427 None:intergenic 40.0%
TGCTACACCAGAACTAAACA+TGG - chr5_4:6447412-6447431 None:intergenic 40.0%
TCAAAAACCATGGGTCTAAG+AGG + chr5_4:6447465-6447484 Msa0833250:CDS 40.0%
CAAAAACCATGGGTCTAAGA+GGG + chr5_4:6447466-6447485 Msa0833250:CDS 40.0%
AAAAACCATGGGTCTAAGAG+GGG + chr5_4:6447467-6447486 Msa0833250:CDS 40.0%
ATCAGTTATCCAAACAGCCA+TGG - chr5_4:6447496-6447515 None:intergenic 40.0%
! ATGGTGGGATCAGAGTATTA+TGG + chr5_4:6447593-6447612 Msa0833250:CDS 40.0%
! GGGATCAGAGTATTATGGAT+TGG + chr5_4:6447598-6447617 Msa0833250:CDS 40.0%
TAAAACTCCCAATGCTTGCT+TGG - chr5_4:6447724-6447743 None:intergenic 40.0%
TTTCTCAGTGATGCTGTCAT+TGG + chr5_4:6447773-6447792 Msa0833250:CDS 40.0%
!! CTAGGAATAGGTTTTATCGC+AGG + chr5_4:6447876-6447895 Msa0833250:CDS 40.0%
CATTTATAGTGCCCCTATCA+TGG - chr5_4:6447959-6447978 None:intergenic 40.0%
!! CATGATAGGGGCACTATAAA+TGG + chr5_4:6447957-6447976 Msa0833250:CDS 40.0%
!! AATGTTGTTGATGGCTCTAG+TGG + chr5_4:6447992-6448011 Msa0833250:CDS 40.0%
TTTGCATACATACCCAACCA+AGG - chr5_4:6448074-6448093 None:intergenic 40.0%
GGTATGTATGCAAATCTAGG+CGG + chr5_4:6448080-6448099 Msa0833250:CDS 40.0%
GTATGTATGCAAATCTAGGC+GGG + chr5_4:6448081-6448100 Msa0833250:CDS 40.0%
!!! TCTAGGCGGGTTTTATTTTC+TGG + chr5_4:6448094-6448113 Msa0833250:CDS 40.0%
AAGGCAAAAACAACACCAAG+GGG - chr5_4:6448125-6448144 None:intergenic 40.0%
GAAGGCAAAAACAACACCAA+GGG - chr5_4:6448126-6448145 None:intergenic 40.0%
TGAAGGCAAAAACAACACCA+AGG - chr5_4:6448127-6448146 None:intergenic 40.0%
CTCTTCTTTGGACTTCTTAC+TGG + chr5_4:6448170-6448189 Msa0833250:CDS 40.0%
CACAGGGAACTTCTTTAACT+TGG - chr5_4:6448289-6448308 None:intergenic 40.0%
!!! TTTTGGGTTTGTAATGGTGG+AGG - chr5_4:6446892-6446911 None:intergenic 40.0%
! CTGGATTTGCGTCACATTTT+TGG - chr5_4:6446909-6446928 None:intergenic 40.0%
TCAGAGGAGCTAAGAATTCA+AGG + chr5_4:6446943-6446962 Msa0833250:CDS 40.0%
TTCACTTTCGCTAACGTTAC+TGG + chr5_4:6447081-6447100 Msa0833250:CDS 40.0%
!! TCATGAGAAGTGTCTTGTGA+AGG - chr5_4:6447185-6447204 None:intergenic 40.0%
CAAGAAATTTCCACTGTTGC+TGG + chr5_4:6447291-6447310 Msa0833250:CDS 40.0%
AACATAAGTACCAGCAACAG+TGG - chr5_4:6447304-6447323 None:intergenic 40.0%
! CTTCCTACCATGTTTAGTTC+TGG + chr5_4:6447402-6447421 Msa0833250:CDS 40.0%
ACACCAGAACTAAACATGGT+AGG - chr5_4:6447408-6447427 None:intergenic 40.0%
TGCTACACCAGAACTAAACA+TGG - chr5_4:6447412-6447431 None:intergenic 40.0%
TCAAAAACCATGGGTCTAAG+AGG + chr5_4:6447465-6447484 Msa0833250:CDS 40.0%
CAAAAACCATGGGTCTAAGA+GGG + chr5_4:6447466-6447485 Msa0833250:CDS 40.0%
AAAAACCATGGGTCTAAGAG+GGG + chr5_4:6447467-6447486 Msa0833250:CDS 40.0%
ATCAGTTATCCAAACAGCCA+TGG - chr5_4:6447496-6447515 None:intergenic 40.0%
! ATGGTGGGATCAGAGTATTA+TGG + chr5_4:6447593-6447612 Msa0833250:CDS 40.0%
! GGGATCAGAGTATTATGGAT+TGG + chr5_4:6447598-6447617 Msa0833250:CDS 40.0%
TAAAACTCCCAATGCTTGCT+TGG - chr5_4:6447724-6447743 None:intergenic 40.0%
TTTCTCAGTGATGCTGTCAT+TGG + chr5_4:6447773-6447792 Msa0833250:CDS 40.0%
!! CTAGGAATAGGTTTTATCGC+AGG + chr5_4:6447876-6447895 Msa0833250:CDS 40.0%
CATTTATAGTGCCCCTATCA+TGG - chr5_4:6447959-6447978 None:intergenic 40.0%
!! CATGATAGGGGCACTATAAA+TGG + chr5_4:6447957-6447976 Msa0833250:CDS 40.0%
!! AATGTTGTTGATGGCTCTAG+TGG + chr5_4:6447992-6448011 Msa0833250:CDS 40.0%
TTTGCATACATACCCAACCA+AGG - chr5_4:6448074-6448093 None:intergenic 40.0%
GGTATGTATGCAAATCTAGG+CGG + chr5_4:6448080-6448099 Msa0833250:CDS 40.0%
GTATGTATGCAAATCTAGGC+GGG + chr5_4:6448081-6448100 Msa0833250:CDS 40.0%
!!! TCTAGGCGGGTTTTATTTTC+TGG + chr5_4:6448094-6448113 Msa0833250:CDS 40.0%
AAGGCAAAAACAACACCAAG+GGG - chr5_4:6448125-6448144 None:intergenic 40.0%
GAAGGCAAAAACAACACCAA+GGG - chr5_4:6448126-6448145 None:intergenic 40.0%
TGAAGGCAAAAACAACACCA+AGG - chr5_4:6448127-6448146 None:intergenic 40.0%
CTCTTCTTTGGACTTCTTAC+TGG + chr5_4:6448170-6448189 Msa0833250:CDS 40.0%
CACAGGGAACTTCTTTAACT+TGG - chr5_4:6448289-6448308 None:intergenic 40.0%
TGCTGTTGTGATTGCTGTCT+TGG - chr5_4:6447013-6447032 None:intergenic 45.0%
!!! ACAGCATTTTTGGGTCGACT+AGG + chr5_4:6447027-6447046 Msa0833250:CDS 45.0%
! ACTGGCTTCTCTGTCTTGAA+TGG + chr5_4:6447099-6447118 Msa0833250:CDS 45.0%
TCTGTCTTGAATGGTCTCTG+TGG + chr5_4:6447108-6447127 Msa0833250:CDS 45.0%
CTGTCCACAAATAGGTTCCA+TGG - chr5_4:6447136-6447155 None:intergenic 45.0%
CATGGAACCTATTTGTGGAC+AGG + chr5_4:6447134-6447153 Msa0833250:CDS 45.0%
!! AGAATCGAAAGGTATTGGCG+TGG + chr5_4:6447559-6447578 Msa0833250:CDS 45.0%
!! AAGGTATTGGCGTGGAAAGA+AGG + chr5_4:6447567-6447586 Msa0833250:CDS 45.0%
GTCTCAACACATGTATGGAG+TGG + chr5_4:6447652-6447671 Msa0833250:CDS 45.0%
TCAACACATGTATGGAGTGG+TGG + chr5_4:6447655-6447674 Msa0833250:CDS 45.0%
GCAACTCTTCAGCCATGATA+GGG + chr5_4:6447944-6447963 Msa0833250:CDS 45.0%
CAACTCTTCAGCCATGATAG+GGG + chr5_4:6447945-6447964 Msa0833250:CDS 45.0%
TTTCCATTGGCAGTTTGTGG+AGG + chr5_4:6448026-6448045 Msa0833250:CDS 45.0%
! TTTGTGGAGGCATAGTTCGA+GGG + chr5_4:6448039-6448058 Msa0833250:CDS 45.0%
TTTGCCTTCAAGCTTCGTCT+TGG + chr5_4:6448137-6448156 Msa0833250:CDS 45.0%
GTCCAAAGAAGAGTCCAACA+AGG - chr5_4:6448163-6448182 None:intergenic 45.0%
CATTGCTAGGATAAAGTGGG+TGG + chr5_4:6448226-6448245 Msa0833250:CDS 45.0%
! TCATTTTCACGAGCCTCAGT+TGG - chr5_4:6448326-6448345 None:intergenic 45.0%
TGCTGTTGTGATTGCTGTCT+TGG - chr5_4:6447013-6447032 None:intergenic 45.0%
!!! ACAGCATTTTTGGGTCGACT+AGG + chr5_4:6447027-6447046 Msa0833250:CDS 45.0%
! ACTGGCTTCTCTGTCTTGAA+TGG + chr5_4:6447099-6447118 Msa0833250:CDS 45.0%
TCTGTCTTGAATGGTCTCTG+TGG + chr5_4:6447108-6447127 Msa0833250:CDS 45.0%
CTGTCCACAAATAGGTTCCA+TGG - chr5_4:6447136-6447155 None:intergenic 45.0%
CATGGAACCTATTTGTGGAC+AGG + chr5_4:6447134-6447153 Msa0833250:CDS 45.0%
!! AGAATCGAAAGGTATTGGCG+TGG + chr5_4:6447559-6447578 Msa0833250:CDS 45.0%
!! AAGGTATTGGCGTGGAAAGA+AGG + chr5_4:6447567-6447586 Msa0833250:CDS 45.0%
GTCTCAACACATGTATGGAG+TGG + chr5_4:6447652-6447671 Msa0833250:CDS 45.0%
TCAACACATGTATGGAGTGG+TGG + chr5_4:6447655-6447674 Msa0833250:CDS 45.0%
GCAACTCTTCAGCCATGATA+GGG + chr5_4:6447944-6447963 Msa0833250:CDS 45.0%
CAACTCTTCAGCCATGATAG+GGG + chr5_4:6447945-6447964 Msa0833250:CDS 45.0%
TTTCCATTGGCAGTTTGTGG+AGG + chr5_4:6448026-6448045 Msa0833250:CDS 45.0%
! TTTGTGGAGGCATAGTTCGA+GGG + chr5_4:6448039-6448058 Msa0833250:CDS 45.0%
TTTGCCTTCAAGCTTCGTCT+TGG + chr5_4:6448137-6448156 Msa0833250:CDS 45.0%
GTCCAAAGAAGAGTCCAACA+AGG - chr5_4:6448163-6448182 None:intergenic 45.0%
CATTGCTAGGATAAAGTGGG+TGG + chr5_4:6448226-6448245 Msa0833250:CDS 45.0%
! TCATTTTCACGAGCCTCAGT+TGG - chr5_4:6448326-6448345 None:intergenic 45.0%
! AATGGTGGAGGAGGTGACAT+TGG - chr5_4:6446880-6446899 None:intergenic 50.0%
! TGGGTTTGTAATGGTGGAGG+AGG - chr5_4:6446889-6446908 None:intergenic 50.0%
! GGTGCCATGGAACCTATTTG+TGG + chr5_4:6447129-6447148 Msa0833250:CDS 50.0%
CCATGAGCCTGTCCACAAAT+AGG - chr5_4:6447144-6447163 None:intergenic 50.0%
CCTATTTGTGGACAGGCTCA+TGG + chr5_4:6447141-6447160 Msa0833250:CDS 50.0%
! GTATTGGCGTGGAAAGAAGG+AGG + chr5_4:6447570-6447589 Msa0833250:CDS 50.0%
GTGGAAAGAAGGAGGATGGT+GGG + chr5_4:6447578-6447597 Msa0833250:CDS 50.0%
GCAAACGCCAAGCAAGCATT+GGG + chr5_4:6447714-6447733 Msa0833250:CDS 50.0%
!! GGTTTTATCGCAGGTTGCAC+CGG + chr5_4:6447885-6447904 Msa0833250:CDS 50.0%
GGCAACTCTTCAGCCATGAT+AGG + chr5_4:6447943-6447962 Msa0833250:CDS 50.0%
ATGCCTCCACAAACTGCCAA+TGG - chr5_4:6448032-6448051 None:intergenic 50.0%
! GTTTGTGGAGGCATAGTTCG+AGG + chr5_4:6448038-6448057 Msa0833250:CDS 50.0%
!!! TATTTTCTGGCTCTGCCCCT+TGG + chr5_4:6448107-6448126 Msa0833250:CDS 50.0%
AAGGCCAAGACGAAGCTTGA+AGG - chr5_4:6448144-6448163 None:intergenic 50.0%
!! AAGCTTCGTCTTGGCCTTGT+TGG + chr5_4:6448146-6448165 Msa0833250:CDS 50.0%
!! GGCCTTGTTGGACTCTTCTT+TGG + chr5_4:6448158-6448177 Msa0833250:CDS 50.0%
GGTACTTCTGCGTCATCACA+GGG - chr5_4:6448305-6448324 None:intergenic 50.0%
TGGTACTTCTGCGTCATCAC+AGG - chr5_4:6448306-6448325 None:intergenic 50.0%
!! TGACGCAGAAGTACCAACTG+AGG + chr5_4:6448310-6448329 Msa0833250:CDS 50.0%
! AATGGTGGAGGAGGTGACAT+TGG - chr5_4:6446880-6446899 None:intergenic 50.0%
! TGGGTTTGTAATGGTGGAGG+AGG - chr5_4:6446889-6446908 None:intergenic 50.0%
! GGTGCCATGGAACCTATTTG+TGG + chr5_4:6447129-6447148 Msa0833250:CDS 50.0%
CCATGAGCCTGTCCACAAAT+AGG - chr5_4:6447144-6447163 None:intergenic 50.0%
CCTATTTGTGGACAGGCTCA+TGG + chr5_4:6447141-6447160 Msa0833250:CDS 50.0%
! GTATTGGCGTGGAAAGAAGG+AGG + chr5_4:6447570-6447589 Msa0833250:CDS 50.0%
GTGGAAAGAAGGAGGATGGT+GGG + chr5_4:6447578-6447597 Msa0833250:CDS 50.0%
GCAAACGCCAAGCAAGCATT+GGG + chr5_4:6447714-6447733 Msa0833250:CDS 50.0%
!! GGTTTTATCGCAGGTTGCAC+CGG + chr5_4:6447885-6447904 Msa0833250:CDS 50.0%
GGCAACTCTTCAGCCATGAT+AGG + chr5_4:6447943-6447962 Msa0833250:CDS 50.0%
ATGCCTCCACAAACTGCCAA+TGG - chr5_4:6448032-6448051 None:intergenic 50.0%
! GTTTGTGGAGGCATAGTTCG+AGG + chr5_4:6448038-6448057 Msa0833250:CDS 50.0%
!!! TATTTTCTGGCTCTGCCCCT+TGG + chr5_4:6448107-6448126 Msa0833250:CDS 50.0%
AAGGCCAAGACGAAGCTTGA+AGG - chr5_4:6448144-6448163 None:intergenic 50.0%
!! AAGCTTCGTCTTGGCCTTGT+TGG + chr5_4:6448146-6448165 Msa0833250:CDS 50.0%
!! GGCCTTGTTGGACTCTTCTT+TGG + chr5_4:6448158-6448177 Msa0833250:CDS 50.0%
GGTACTTCTGCGTCATCACA+GGG - chr5_4:6448305-6448324 None:intergenic 50.0%
TGGTACTTCTGCGTCATCAC+AGG - chr5_4:6448306-6448325 None:intergenic 50.0%
!! TGACGCAGAAGTACCAACTG+AGG + chr5_4:6448310-6448329 Msa0833250:CDS 50.0%
GTGGAGGAGGTGACATTGGT+TGG - chr5_4:6446876-6446895 None:intergenic 55.0%
TCTGAAAGCAGCGTCTTCGC+TGG - chr5_4:6446928-6446947 None:intergenic 55.0%
! AGCGAAGACGCTGCTTTCAG+AGG + chr5_4:6446927-6446946 Msa0833250:CDS 55.0%
CTAGGTGAGCTCAGCTTAGC+CGG + chr5_4:6447045-6447064 Msa0833250:CDS 55.0%
! GAATGGTCTCTGTGGTGCCA+TGG + chr5_4:6447116-6447135 Msa0833250:CDS 55.0%
GGAAACCCCTCTTAGACCCA+TGG - chr5_4:6447475-6447494 None:intergenic 55.0%
GGGTCTAAGAGGGGTTTCCA+TGG + chr5_4:6447476-6447495 Msa0833250:CDS 55.0%
GAGGGGTTTCCATGGCTGTT+TGG + chr5_4:6447484-6447503 Msa0833250:CDS 55.0%
TGGCGTGGAAAGAAGGAGGA+TGG + chr5_4:6447574-6447593 Msa0833250:CDS 55.0%
CGTGGAAAGAAGGAGGATGG+TGG + chr5_4:6447577-6447596 Msa0833250:CDS 55.0%
ATGCTTGCTTGGCGTTTGCG+AGG - chr5_4:6447713-6447732 None:intergenic 55.0%
CGCAAACGCCAAGCAAGCAT+TGG + chr5_4:6447713-6447732 Msa0833250:CDS 55.0%
GACACGGGTGGAAACACAAG+TGG - chr5_4:6447799-6447818 None:intergenic 55.0%
! AAGCTCGTTTGAGACACGGG+TGG - chr5_4:6447811-6447830 None:intergenic 55.0%
ACCCGTGTCTCAAACGAGCT+TGG + chr5_4:6447810-6447829 Msa0833250:CDS 55.0%
! GCCAAGCTCGTTTGAGACAC+GGG - chr5_4:6447814-6447833 None:intergenic 55.0%
! CGCCAAGCTCGTTTGAGACA+CGG - chr5_4:6447815-6447834 None:intergenic 55.0%
! GCGCGTGTGTCTCTAGGAAT+AGG + chr5_4:6447864-6447883 Msa0833250:CDS 55.0%
!! CTTGGTGATGGTGGCTGCAA+GGG + chr5_4:6447911-6447930 Msa0833250:CDS 55.0%
!! TTGGTGATGGTGGCTGCAAG+GGG + chr5_4:6447912-6447931 Msa0833250:CDS 55.0%
TGGTGGCTGCAAGGGGAATT+TGG + chr5_4:6447919-6447938 Msa0833250:CDS 55.0%
GGTGGCTGCAAGGGGAATTT+GGG + chr5_4:6447920-6447939 Msa0833250:CDS 55.0%
GTGGCTGCAAGGGGAATTTG+GGG + chr5_4:6447921-6447940 Msa0833250:CDS 55.0%
TGGCTGCAAGGGGAATTTGG+GGG + chr5_4:6447922-6447941 Msa0833250:CDS 55.0%
GTGGAGGAGGTGACATTGGT+TGG - chr5_4:6446876-6446895 None:intergenic 55.0%
TCTGAAAGCAGCGTCTTCGC+TGG - chr5_4:6446928-6446947 None:intergenic 55.0%
! AGCGAAGACGCTGCTTTCAG+AGG + chr5_4:6446927-6446946 Msa0833250:CDS 55.0%
CTAGGTGAGCTCAGCTTAGC+CGG + chr5_4:6447045-6447064 Msa0833250:CDS 55.0%
! GAATGGTCTCTGTGGTGCCA+TGG + chr5_4:6447116-6447135 Msa0833250:CDS 55.0%
GGAAACCCCTCTTAGACCCA+TGG - chr5_4:6447475-6447494 None:intergenic 55.0%
GGGTCTAAGAGGGGTTTCCA+TGG + chr5_4:6447476-6447495 Msa0833250:CDS 55.0%
GAGGGGTTTCCATGGCTGTT+TGG + chr5_4:6447484-6447503 Msa0833250:CDS 55.0%
TGGCGTGGAAAGAAGGAGGA+TGG + chr5_4:6447574-6447593 Msa0833250:CDS 55.0%
CGTGGAAAGAAGGAGGATGG+TGG + chr5_4:6447577-6447596 Msa0833250:CDS 55.0%
ATGCTTGCTTGGCGTTTGCG+AGG - chr5_4:6447713-6447732 None:intergenic 55.0%
CGCAAACGCCAAGCAAGCAT+TGG + chr5_4:6447713-6447732 Msa0833250:CDS 55.0%
GACACGGGTGGAAACACAAG+TGG - chr5_4:6447799-6447818 None:intergenic 55.0%
! AAGCTCGTTTGAGACACGGG+TGG - chr5_4:6447811-6447830 None:intergenic 55.0%
ACCCGTGTCTCAAACGAGCT+TGG + chr5_4:6447810-6447829 Msa0833250:CDS 55.0%
! GCCAAGCTCGTTTGAGACAC+GGG - chr5_4:6447814-6447833 None:intergenic 55.0%
! CGCCAAGCTCGTTTGAGACA+CGG - chr5_4:6447815-6447834 None:intergenic 55.0%
! GCGCGTGTGTCTCTAGGAAT+AGG + chr5_4:6447864-6447883 Msa0833250:CDS 55.0%
!! CTTGGTGATGGTGGCTGCAA+GGG + chr5_4:6447911-6447930 Msa0833250:CDS 55.0%
!! TTGGTGATGGTGGCTGCAAG+GGG + chr5_4:6447912-6447931 Msa0833250:CDS 55.0%
TGGTGGCTGCAAGGGGAATT+TGG + chr5_4:6447919-6447938 Msa0833250:CDS 55.0%
GGTGGCTGCAAGGGGAATTT+GGG + chr5_4:6447920-6447939 Msa0833250:CDS 55.0%
GTGGCTGCAAGGGGAATTTG+GGG + chr5_4:6447921-6447940 Msa0833250:CDS 55.0%
TGGCTGCAAGGGGAATTTGG+GGG + chr5_4:6447922-6447941 Msa0833250:CDS 55.0%
CTTGCTTGGCGTTTGCGAGG+TGG - chr5_4:6447710-6447729 None:intergenic 60.0%
! CTAGAGACACACGCGCTGAC+CGG - chr5_4:6447860-6447879 None:intergenic 60.0%
! TTGCACCGGCAGCTTGGTGA+TGG + chr5_4:6447899-6447918 Msa0833250:CDS 60.0%
AGCCACCATCACCAAGCTGC+CGG - chr5_4:6447907-6447926 None:intergenic 60.0%
!! GCTTGGTGATGGTGGCTGCA+AGG + chr5_4:6447910-6447929 Msa0833250:CDS 60.0%
TTCGAGGGACAGCTCGACCT+TGG + chr5_4:6448054-6448073 Msa0833250:CDS 60.0%
AGGGACAGCTCGACCTTGGT+TGG + chr5_4:6448058-6448077 Msa0833250:CDS 60.0%
GGGACAGCTCGACCTTGGTT+GGG + chr5_4:6448059-6448078 Msa0833250:CDS 60.0%
GTGGGTGGAAGAAGCTGCCA+AGG + chr5_4:6448241-6448260 Msa0833250:CDS 60.0%
CTTGCTTGGCGTTTGCGAGG+TGG - chr5_4:6447710-6447729 None:intergenic 60.0%
! CTAGAGACACACGCGCTGAC+CGG - chr5_4:6447860-6447879 None:intergenic 60.0%
! TTGCACCGGCAGCTTGGTGA+TGG + chr5_4:6447899-6447918 Msa0833250:CDS 60.0%
AGCCACCATCACCAAGCTGC+CGG - chr5_4:6447907-6447926 None:intergenic 60.0%
!! GCTTGGTGATGGTGGCTGCA+AGG + chr5_4:6447910-6447929 Msa0833250:CDS 60.0%
TTCGAGGGACAGCTCGACCT+TGG + chr5_4:6448054-6448073 Msa0833250:CDS 60.0%
AGGGACAGCTCGACCTTGGT+TGG + chr5_4:6448058-6448077 Msa0833250:CDS 60.0%
GGGACAGCTCGACCTTGGTT+GGG + chr5_4:6448059-6448078 Msa0833250:CDS 60.0%
GTGGGTGGAAGAAGCTGCCA+AGG + chr5_4:6448241-6448260 Msa0833250:CDS 60.0%
GGTGAGCTCAGCTTAGCCGG+TGG + chr5_4:6447048-6447067 Msa0833250:CDS 65.0%
ACCAAGCCGACCGAGCGTAC+CGG + chr5_4:6447838-6447857 Msa0833250:CDS 65.0%
CGGTCAGCGCGTGTGTCTCT+AGG + chr5_4:6447858-6447877 Msa0833250:CDS 65.0%
!! CACCGGCAGCTTGGTGATGG+TGG + chr5_4:6447902-6447921 Msa0833250:CDS 65.0%
GGTGAGCTCAGCTTAGCCGG+TGG + chr5_4:6447048-6447067 Msa0833250:CDS 65.0%
ACCAAGCCGACCGAGCGTAC+CGG + chr5_4:6447838-6447857 Msa0833250:CDS 65.0%
CGGTCAGCGCGTGTGTCTCT+AGG + chr5_4:6447858-6447877 Msa0833250:CDS 65.0%
!! CACCGGCAGCTTGGTGATGG+TGG + chr5_4:6447902-6447921 Msa0833250:CDS 65.0%
AGCTTAGCCGGTGGCGCGCT+CGG + chr5_4:6447057-6447076 Msa0833250:CDS 70.0%
AGTGAACCCGAGCGCGCCAC+CGG - chr5_4:6447067-6447086 None:intergenic 70.0%
ACCGGTACGCTCGGTCGGCT+TGG - chr5_4:6447842-6447861 None:intergenic 70.0%
CGCTGACCGGTACGCTCGGT+CGG - chr5_4:6447847-6447866 None:intergenic 70.0%
! CACGCGCTGACCGGTACGCT+CGG - chr5_4:6447851-6447870 None:intergenic 70.0%
! CGCAGGTTGCACCGGCAGCT+TGG + chr5_4:6447893-6447912 Msa0833250:CDS 70.0%
AGCTTAGCCGGTGGCGCGCT+CGG + chr5_4:6447057-6447076 Msa0833250:CDS 70.0%
AGTGAACCCGAGCGCGCCAC+CGG - chr5_4:6447067-6447086 None:intergenic 70.0%
ACCGGTACGCTCGGTCGGCT+TGG - chr5_4:6447842-6447861 None:intergenic 70.0%
CGCTGACCGGTACGCTCGGT+CGG - chr5_4:6447847-6447866 None:intergenic 70.0%
! CACGCGCTGACCGGTACGCT+CGG - chr5_4:6447851-6447870 None:intergenic 70.0%
! CGCAGGTTGCACCGGCAGCT+TGG + chr5_4:6447893-6447912 Msa0833250:CDS 70.0%
GCTTAGCCGGTGGCGCGCTC+GGG + chr5_4:6447058-6447077 Msa0833250:CDS 75.0%
GCTTAGCCGGTGGCGCGCTC+GGG + chr5_4:6447058-6447077 Msa0833250:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6446820 6448355 6446820 ID=Msa0833250;Name=Msa0833250
chr5_4 mRNA 6446820 6448355 6446820 ID=Msa0833250-mRNA-1;Parent=Msa0833250;Name=Msa0833250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|511
chr5_4 exon 6446820 6448355 6446820 ID=Msa0833250-mRNA-1:exon:2796;Parent=Msa0833250-mRNA-1
chr5_4 CDS 6446820 6448355 6446820 ID=Msa0833250-mRNA-1:cds;Parent=Msa0833250-mRNA-1
Gene Sequence

>Msa0833250

ATGTCATCAACATGCAAATTAGGAGAAAACCTTAGCTCAAACCATAAAACTAACCAACCAATGTCACCTCCTCCACCATTACAAACCCAAAAATGTGACGCAAATCCAGCGAAGACGCTGCTTTCAGAGGAGCTAAGAATTCAAGGAAGATTAGCTTTTCCAATGATGTTGATGAATTTAGCTTGGTTTGCCAAGACAGCAATCACAACAGCATTTTTGGGTCGACTAGGTGAGCTCAGCTTAGCCGGTGGCGCGCTCGGGTTCACTTTCGCTAACGTTACTGGCTTCTCTGTCTTGAATGGTCTCTGTGGTGCCATGGAACCTATTTGTGGACAGGCTCATGGAGCTAAAAACGTGAGACTCCTTCACAAGACACTTCTCATGACAATTGTACTGTTGCTATTGGTAACAATTCCTATTACTTTCATGTGGCTTCATATTGACAAAATTTTGATTCATTTTGGCCAACAACAAGAAATTTCCACTGTTGCTGGTACTTATGTTTACTATCTCATACCTGATTTGTTTGTTATGTCACTCTTGTGTCCCTTAAAAGCTTACTTGAGCTCTCAAAGCATCACTCTTCCTACCATGTTTAGTTCTGGTGTAGCACTTGCTTTTCATGTTCCTGTTAACATACTACTCTCAAAAACCATGGGTCTAAGAGGGGTTTCCATGGCTGTTTGGATAACTGATCTTATTGTTGTTGTTCTTCTGGCCATTTATGTTTTAATTCTAGAGAATCGAAAGGTATTGGCGTGGAAAGAAGGAGGATGGTGGGATCAGAGTATTATGGATTGGATTAGGCTAATCAAGCTATCTGGATCTTGTTGTCTCAACACATGTATGGAGTGGTGGTGCTATGAAATTCTAGTCTTGCTTACTGGCCACCTCGCAAACGCCAAGCAAGCATTGGGAGTTTTAGCCATTGTGTTGAACTTTGACTATTTACTTTTCTCAGTGATGCTGTCATTGGCCACTTGTGTTTCCACCCGTGTCTCAAACGAGCTTGGCGCAAACCAAGCCGACCGAGCGTACCGGTCAGCGCGTGTGTCTCTAGGAATAGGTTTTATCGCAGGTTGCACCGGCAGCTTGGTGATGGTGGCTGCAAGGGGAATTTGGGGGCAACTCTTCAGCCATGATAGGGGCACTATAAATGGAGTAAAAAAGACAATGTTGTTGATGGCTCTAGTGGAGTTGTTTAATTTTCCATTGGCAGTTTGTGGAGGCATAGTTCGAGGGACAGCTCGACCTTGGTTGGGTATGTATGCAAATCTAGGCGGGTTTTATTTTCTGGCTCTGCCCCTTGGTGTTGTTTTTGCCTTCAAGCTTCGTCTTGGCCTTGTTGGACTCTTCTTTGGACTTCTTACTGGTATTGTTGTTTGCTTGTCATTATTATTAGTATTCATTGCTAGGATAAAGTGGGTGGAAGAAGCTGCCAAGGCACAAATATTAGCAAGTAACGACCAAGTTAAAGAAGTTCCCTGTGATGACGCAGAAGTACCAACTGAGGCTCGTGAAAATGACAAAGTGTAA
Protein sequence

>Msa0833250

MSSTCKLGENLSSNHKTNQPMSPPPPLQTQKCDANPAKTLLSEELRIQGRLAFPMMLMNLAWFAKTAITTAFLGRLGELSLAGGALGFTFANVTGFSVLNGLCGAMEPICGQAHGAKNVRLLHKTLLMTIVLLLLVTIPITFMWLHIDKILIHFGQQQEISTVAGTYVYYLIPDLFVMSLLCPLKAYLSSQSITLPTMFSSGVALAFHVPVNILLSKTMGLRGVSMAVWITDLIVVVLLAIYVLILENRKVLAWKEGGWWDQSIMDWIRLIKLSGSCCLNTCMEWWCYEILVLLTGHLANAKQALGVLAIVLNFDYLLFSVMLSLATCVSTRVSNELGANQADRAYRSARVSLGIGFIAGCTGSLVMVAARGIWGQLFSHDRGTINGVKKTMLLMALVELFNFPLAVCGGIVRGTARPWLGMYANLGGFYFLALPLGVVFAFKLRLGLVGLFFGLLTGIVVCLSLLLVFIARIKWVEEAAKAQILASNDQVKEVPCDDAEVPTEARENDKV*