AlfalfaGEDB | Msa0833250

M. sativa cultivar zhongmu-4 / Msa0833250

KEGG: K03327
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006855 GO:0008150 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010119 GO:0015238 GO:0015691 GO:0015893 GO:0016020 GO:0022857 GO:0030001 GO:0035690 GO:0042221 GO:0042493 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051716 GO:0055085 GO:0065007 GO:0065008 GO:0070838 GO:0070887 GO:0071241 GO:0071244 GO:0071944 GO:0072347 GO:0072511 GO:1901700 GO:1901701 GO:1902456
PFAM: PF01554.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833250	XP_003611216.1	97.065	511	15	0	1	511	1	511	0.0	1011

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833250	sp\|O49660\|DTX56_ARATH	58.664	479	196	2	16	493	3	480	0.0	563

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833250	G7KG46	97.065	511	15	0	1	511	1	511	0.0	1011

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095920	Msa0833250	0.801605	8.319755e-49	-8.615850e-47
Msa0833250	Msa1412370	0.805437	1.335485e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833250	MtrunA17_Chr5g0398571	98.408	314	5	0	198	511	1	314	0.0	627
Msa0833250	MtrunA17_Chr6g0467321	39.286	476	275	5	36	497	35	510	7.03e-104	321
Msa0833250	MtrunA17_Chr5g0428251	37.204	465	277	5	44	502	56	511	7.48e-100	311
Msa0833250	MtrunA17_Chr3g0140931	37.372	487	284	6	13	486	15	493	2.62e-99	310
Msa0833250	MtrunA17_Chr5g0397991	37.179	468	287	4	20	485	44	506	7.63e-98	306
Msa0833250	MtrunA17_Chr1g0203791	37.778	495	291	7	25	506	39	529	2.60e-97	304
Msa0833250	MtrunA17_Chr6g0473701	35.714	490	308	5	24	510	109	594	1.99e-95	301
Msa0833250	MtrunA17_Chr8g0389801	36.569	443	278	2	43	485	23	462	1.82e-92	289
Msa0833250	MtrunA17_Chr4g0013031	32.829	463	308	2	41	501	64	525	7.86e-90	285
Msa0833250	MtrunA17_Chr2g0295631	32.479	468	289	3	43	489	20	481	3.09e-81	261

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833250	AT4G22790.1	58.664	479	196	2	16	493	3	480	0.0	563
Msa0833250	AT1G71870.1	37.895	475	278	5	35	492	15	489	3.69e-99	308
Msa0833250	AT4G29140.1	34.731	501	292	8	24	500	34	523	7.82e-95	298
Msa0833250	AT1G58340.1	39.520	458	266	3	43	495	57	508	2.58e-93	294
Msa0833250	AT5G19700.1	37.310	461	284	3	36	494	28	485	5.12e-93	293
Msa0833250	AT5G52050.1	31.703	511	332	6	11	510	1	505	2.70e-90	285
Msa0833250	AT4G23030.1	36.952	479	291	5	30	502	15	488	1.25e-83	268
Msa0833250	AT2G38510.1	31.712	473	318	4	41	511	3	472	5.30e-83	266
Msa0833250	AT5G49130.1	33.970	471	298	2	35	492	17	487	5.90e-83	266
Msa0833250	AT2G04040.1	33.184	446	291	3	41	483	24	465	7.02e-68	226

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Find 121 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATAAGTACCAGCAACAG+TGG	0.598946	5_4:-6447301	None:intergenic
AAGCTCGTTTGAGACACGGG+TGG	0.702539	5_4:-6447808	None:intergenic
AAGCTTCGTCTTGGCCTTGT+TGG	0.336381	5_4:+6448146	Msa0833250:CDS
AAGGCCAAGACGAAGCTTGA+AGG	0.508427	5_4:-6448141	None:intergenic
AAGGTATTGGCGTGGAAAGA+AGG	0.518806	5_4:+6447567	Msa0833250:CDS
AATGGTGGAGGAGGTGACAT+TGG	0.553401	5_4:-6446877	None:intergenic
AATGTTGTTGATGGCTCTAG+TGG	0.559949	5_4:+6447992	Msa0833250:CDS
ACACCAGAACTAAACATGGT+AGG	0.622764	5_4:-6447405	None:intergenic
ACCAAGCCGACCGAGCGTAC+CGG	0.581822	5_4:+6447838	Msa0833250:CDS
ACCCGTGTCTCAAACGAGCT+TGG	0.500843	5_4:+6447810	Msa0833250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTCATCAACATGCAAATT+AGG	+	chr5_4:6446820-6446839	Msa0833250:CDS	30.0%
GCTAAATTCATCAACATCAT+TGG	-	chr5_4:6446982-6447001	None:intergenic	30.0%
CAATTCCTATTACTTTCATG+TGG	+	chr5_4:6447229-6447248	Msa0833250:CDS	30.0%
AGTGACATAACAAACAAATC+AGG	-	chr5_4:6447339-6447358	None:intergenic	30.0%
TTTGAGAGTAGTATGTTAAC+AGG	-	chr5_4:6447450-6447469	None:intergenic	30.0%
ATACTACTCTCAAAAACCAT+GGG	+	chr5_4:6447456-6447475	Msa0833250:CDS	30.0%
ATAGTCAAAGTTCAACACAA+TGG	-	chr5_4:6447748-6447767	None:intergenic	30.0%
TATTCATTGCTAGGATAAAG+TGG	+	chr5_4:6448222-6448241	Msa0833250:CDS	30.0%
ATTCATTGCTAGGATAAAGT+GGG	+	chr5_4:6448223-6448242	Msa0833250:CDS	30.0%
ATGTCATCAACATGCAAATT+AGG	+	chr5_4:6446820-6446839	Msa0833250:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	6446820	6448355	6446820	ID=Msa0833250;Name=Msa0833250
chr5_4	mRNA	6446820	6448355	6446820	ID=Msa0833250-mRNA-1;Parent=Msa0833250;Name=Msa0833250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|511
chr5_4	exon	6446820	6448355	6446820	ID=Msa0833250-mRNA-1:exon:2796;Parent=Msa0833250-mRNA-1
chr5_4	CDS	6446820	6448355	6446820	ID=Msa0833250-mRNA-1:cds;Parent=Msa0833250-mRNA-1

Gene Sequence

>Msa0833250

ATGTCATCAACATGCAAATTAGGAGAAAACCTTAGCTCAAACCATAAAACTAACCAACCAATGTCACCTCCTCCACCATTACAAACCCAAAAATGTGACGCAAATCCAGCGAAGACGCTGCTTTCAGAGGAGCTAAGAATTCAAGGAAGATTAGCTTTTCCAATGATGTTGATGAATTTAGCTTGGTTTGCCAAGACAGCAATCACAACAGCATTTTTGGGTCGACTAGGTGAGCTCAGCTTAGCCGGTGGCGCGCTCGGGTTCACTTTCGCTAACGTTACTGGCTTCTCTGTCTTGAATGGTCTCTGTGGTGCCATGGAACCTATTTGTGGACAGGCTCATGGAGCTAAAAACGTGAGACTCCTTCACAAGACACTTCTCATGACAATTGTACTGTTGCTATTGGTAACAATTCCTATTACTTTCATGTGGCTTCATATTGACAAAATTTTGATTCATTTTGGCCAACAACAAGAAATTTCCACTGTTGCTGGTACTTATGTTTACTATCTCATACCTGATTTGTTTGTTATGTCACTCTTGTGTCCCTTAAAAGCTTACTTGAGCTCTCAAAGCATCACTCTTCCTACCATGTTTAGTTCTGGTGTAGCACTTGCTTTTCATGTTCCTGTTAACATACTACTCTCAAAAACCATGGGTCTAAGAGGGGTTTCCATGGCTGTTTGGATAACTGATCTTATTGTTGTTGTTCTTCTGGCCATTTATGTTTTAATTCTAGAGAATCGAAAGGTATTGGCGTGGAAAGAAGGAGGATGGTGGGATCAGAGTATTATGGATTGGATTAGGCTAATCAAGCTATCTGGATCTTGTTGTCTCAACACATGTATGGAGTGGTGGTGCTATGAAATTCTAGTCTTGCTTACTGGCCACCTCGCAAACGCCAAGCAAGCATTGGGAGTTTTAGCCATTGTGTTGAACTTTGACTATTTACTTTTCTCAGTGATGCTGTCATTGGCCACTTGTGTTTCCACCCGTGTCTCAAACGAGCTTGGCGCAAACCAAGCCGACCGAGCGTACCGGTCAGCGCGTGTGTCTCTAGGAATAGGTTTTATCGCAGGTTGCACCGGCAGCTTGGTGATGGTGGCTGCAAGGGGAATTTGGGGGCAACTCTTCAGCCATGATAGGGGCACTATAAATGGAGTAAAAAAGACAATGTTGTTGATGGCTCTAGTGGAGTTGTTTAATTTTCCATTGGCAGTTTGTGGAGGCATAGTTCGAGGGACAGCTCGACCTTGGTTGGGTATGTATGCAAATCTAGGCGGGTTTTATTTTCTGGCTCTGCCCCTTGGTGTTGTTTTTGCCTTCAAGCTTCGTCTTGGCCTTGTTGGACTCTTCTTTGGACTTCTTACTGGTATTGTTGTTTGCTTGTCATTATTATTAGTATTCATTGCTAGGATAAAGTGGGTGGAAGAAGCTGCCAAGGCACAAATATTAGCAAGTAACGACCAAGTTAAAGAAGTTCCCTGTGATGACGCAGAAGTACCAACTGAGGCTCGTGAAAATGACAAAGTGTAA

Protein sequence

>Msa0833250

MSSTCKLGENLSSNHKTNQPMSPPPPLQTQKCDANPAKTLLSEELRIQGRLAFPMMLMNLAWFAKTAITTAFLGRLGELSLAGGALGFTFANVTGFSVLNGLCGAMEPICGQAHGAKNVRLLHKTLLMTIVLLLLVTIPITFMWLHIDKILIHFGQQQEISTVAGTYVYYLIPDLFVMSLLCPLKAYLSSQSITLPTMFSSGVALAFHVPVNILLSKTMGLRGVSMAVWITDLIVVVLLAIYVLILENRKVLAWKEGGWWDQSIMDWIRLIKLSGSCCLNTCMEWWCYEILVLLTGHLANAKQALGVLAIVLNFDYLLFSVMLSLATCVSTRVSNELGANQADRAYRSARVSLGIGFIAGCTGSLVMVAARGIWGQLFSHDRGTINGVKKTMLLMALVELFNFPLAVCGGIVRGTARPWLGMYANLGGFYFLALPLGVVFAFKLRLGLVGLFFGLLTGIVVCLSLLLVFIARIKWVEEAAKAQILASNDQVKEVPCDDAEVPTEARENDKV*