AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1412370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1412370 MtrunA17_Chr5g0398571 98.408 314 5 0 198 511 1 314 0.0 627
Msa1412370 MtrunA17_Chr6g0467321 39.286 476 275 5 36 497 35 510 6.81e-104 321
Msa1412370 MtrunA17_Chr5g0428251 37.204 465 277 5 44 502 56 511 4.07e-100 312
Msa1412370 MtrunA17_Chr3g0140931 37.166 487 285 6 13 486 15 493 5.72e-99 309
Msa1412370 MtrunA17_Chr5g0397991 37.607 468 285 4 20 485 44 506 1.76e-98 307
Msa1412370 MtrunA17_Chr1g0203791 37.778 495 291 7 25 506 39 529 1.22e-97 305
Msa1412370 MtrunA17_Chr6g0473701 35.078 516 311 7 15 510 83 594 2.49e-95 301
Msa1412370 MtrunA17_Chr8g0389801 36.569 443 278 2 43 485 23 462 1.20e-92 290
Msa1412370 MtrunA17_Chr4g0013031 32.829 463 308 2 41 501 64 525 3.50e-90 285
Msa1412370 MtrunA17_Chr2g0295631 32.479 468 289 3 43 489 20 481 1.32e-81 263
Msa1412370 MtrunA17_Chr1g0208791 33.484 442 289 2 43 482 43 481 4.29e-75 245
Msa1412370 MtrunA17_Chr5g0398551 95.161 124 6 0 1 124 1 124 2.70e-73 228
Msa1412370 MtrunA17_Chr6g0483381 32.906 468 307 3 21 482 1 467 3.17e-70 232
Msa1412370 MtrunA17_Chr8g0392601 32.071 449 298 5 38 483 7 451 6.64e-68 226
Msa1412370 MtrunA17_Chr6g0483361 31.702 470 318 2 27 494 9 477 6.92e-68 226
Msa1412370 MtrunA17_Chr6g0449061 32.826 460 296 5 30 483 18 470 1.45e-67 225
Msa1412370 MtrunA17_Chr1g0208821 30.818 477 322 4 9 483 10 480 6.43e-65 218
Msa1412370 MtrunA17_Chr6g0477961 32.719 434 289 2 51 482 35 467 8.82e-64 215
Msa1412370 MtrunA17_Chr2g0317131 30.081 492 318 6 17 495 10 488 2.28e-63 214
Msa1412370 MtrunA17_Chr7g0251331 29.814 483 311 7 30 501 13 478 5.21e-61 207
Msa1412370 MtrunA17_Chr6g0483341 29.350 477 329 5 11 483 9 481 1.38e-60 207
Msa1412370 MtrunA17_Chr2g0317151 31.900 442 296 3 38 477 28 466 1.14e-59 204
Msa1412370 MtrunA17_Chr2g0317161 30.435 460 309 4 38 495 26 476 1.21e-56 196
Msa1412370 MtrunA17_Chr6g0483331 29.047 451 304 7 41 483 10 452 7.00e-56 194
Msa1412370 MtrunA17_Chr6g0483351 32.982 379 251 2 102 478 1 378 1.04e-54 189
Msa1412370 MtrunA17_Chr2g0317171 28.750 480 327 5 18 495 16 482 6.29e-54 189
Msa1412370 MtrunA17_Chr2g0322671 30.474 443 300 4 44 481 53 492 4.83e-53 187
Msa1412370 MtrunA17_Chr4g0024501 29.864 442 305 3 44 483 65 503 1.82e-51 183
Msa1412370 MtrunA17_Chr1g0208891 29.754 447 301 6 43 483 24 463 4.48e-49 176
Msa1412370 MtrunA17_Chr1g0208881 30.180 444 303 4 43 483 25 464 6.13e-49 175
Msa1412370 MtrunA17_Chr7g0251321 30.424 401 244 5 102 497 1 371 1.28e-47 169
Msa1412370 MtrunA17_Chr2g0288281 28.534 382 266 2 105 483 4 381 8.73e-46 164
Msa1412370 MtrunA17_Chr2g0317221 28.916 415 266 7 89 495 3 396 6.01e-45 163
Msa1412370 MtrunA17_Chr6g0487091 32.899 307 202 3 187 493 97 399 6.07e-45 163
Msa1412370 MtrunA17_Chr5g0412771 26.844 488 333 7 12 483 15 494 7.94e-44 162
Msa1412370 MtrunA17_Chr8g0368001 28.381 451 312 6 37 483 45 488 4.77e-42 157
Msa1412370 MtrunA17_Chr6g0483161 27.649 387 256 4 101 485 2 366 1.96e-41 152
Msa1412370 MtrunA17_Chr1g0154441 28.571 385 261 5 105 483 4 380 1.61e-39 147
Msa1412370 MtrunA17_Chr5g0442331 27.549 461 324 5 40 498 43 495 3.61e-39 149
Msa1412370 MtrunA17_Chr5g0412541 26.852 432 313 2 54 484 47 476 1.11e-38 147
Msa1412370 MtrunA17_Chr1g0202761 26.439 469 335 5 38 502 39 501 3.64e-38 146
Msa1412370 MtrunA17_Chr7g0254511 27.115 461 313 6 33 483 20 467 6.09e-37 142
Msa1412370 MtrunA17_Chr6g0468521 27.197 478 317 10 31 493 20 481 1.92e-36 141
Msa1412370 MtrunA17_Chr7g0254521 26.105 475 321 7 43 504 31 488 1.46e-34 135
Msa1412370 MtrunA17_Chr3g0132531 27.202 511 339 11 9 501 7 502 3.37e-33 132
Msa1412370 MtrunA17_Chr6g0468511 26.907 472 318 8 31 489 20 477 4.69e-33 131
Msa1412370 MtrunA17_Chr7g0254531 25.899 417 284 5 102 509 1 401 1.51e-31 125
Msa1412370 MtrunA17_Chr6g0468531 26.667 480 327 8 26 494 18 483 3.91e-31 125
Msa1412370 MtrunA17_Chr5g0412571 28.052 385 274 2 101 484 39 421 6.24e-31 124
Msa1412370 MtrunA17_Chr7g0248051 25.355 493 352 6 8 490 28 514 7.40e-31 125
Msa1412370 MtrunA17_Chr6g0460541 28.424 387 266 5 101 483 4 383 4.95e-28 115
Msa1412370 MtrunA17_Chr6g0483264 33.186 226 142 4 283 502 1 223 1.22e-24 102
Msa1412370 MtrunA17_Chr7g0254501 25.857 321 219 4 186 506 13 314 4.76e-20 90.9
Msa1412370 MtrunA17_Chr6g0483221 32.903 155 98 2 327 478 3 154 3.44e-18 82.4
Msa1412370 MtrunA17_Chr3g0132541 23.347 484 308 11 8 489 8 430 3.55e-18 87.0
Msa1412370 MtrunA17_Chr8g0364751 31.579 171 117 0 312 482 1 171 3.79e-16 76.6
Msa1412370 MtrunA17_Chr6g0483261 33.520 179 110 4 330 502 6 181 4.34e-16 76.6
Msa1412370 MtrunA17_Chr8g0364761 23.273 275 207 3 40 312 50 322 1.01e-15 78.2
Msa1412370 MtrunA17_Chr5g0440661 24.931 361 199 11 105 461 4 296 7.91e-15 75.5
Msa1412370 MtrunA17_Chr6g0460531 31.414 191 124 5 43 228 24 212 1.34e-14 73.9
Msa1412370 MtrunA17_Chr6g0483241 29.655 145 100 1 101 243 2 146 4.28e-14 70.9
Msa1412370 MtrunA17_Chr6g0460521 28.358 201 144 0 283 483 4 204 3.13e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1412370 AT4G22790.1 58.873 479 195 2 16 493 3 480 0.0 564
Msa1412370 AT1G71870.1 37.895 475 278 5 35 492 15 489 2.35e-99 309
Msa1412370 AT4G29140.1 34.930 501 291 8 24 500 34 523 1.11e-95 300
Msa1412370 AT1G58340.1 39.520 458 266 3 43 495 57 508 8.95e-94 295
Msa1412370 AT5G19700.1 37.310 461 284 3 36 494 28 485 2.73e-93 293
Msa1412370 AT5G52050.1 31.703 511 332 6 11 510 1 505 2.32e-90 285
Msa1412370 AT2G38510.1 31.924 473 317 4 41 511 3 472 1.13e-83 268
Msa1412370 AT4G23030.1 36.952 479 291 5 30 502 15 488 1.23e-83 268
Msa1412370 AT5G49130.1 33.970 471 298 2 35 492 17 487 3.58e-83 267
Msa1412370 AT1G66760.2 30.608 477 324 3 18 491 2 474 2.14e-68 228
Msa1412370 AT2G04040.1 33.184 446 291 3 41 483 24 465 4.81e-68 226
Msa1412370 AT1G66760.1 30.603 464 315 3 18 478 2 461 9.93e-66 220
Msa1412370 AT5G52450.1 31.906 467 312 4 38 501 20 483 1.33e-65 220
Msa1412370 AT2G04080.1 30.454 463 315 3 24 483 7 465 2.60e-63 214
Msa1412370 AT2G34360.1 29.622 476 315 8 33 501 16 478 6.74e-63 213
Msa1412370 AT2G04070.1 30.670 463 314 3 24 483 7 465 1.55e-61 209
Msa1412370 AT1G64820.1 31.847 471 300 6 33 492 18 478 1.63e-61 210
Msa1412370 AT1G71140.1 30.131 458 312 4 29 483 12 464 2.40e-61 209
Msa1412370 AT2G04080.2 30.108 465 318 3 24 485 7 467 2.99e-61 208
Msa1412370 AT1G15150.1 29.116 498 332 5 12 502 3 486 3.20e-61 209
Msa1412370 AT1G15160.1 29.851 469 312 4 41 502 28 486 4.37e-61 208
Msa1412370 AT2G04100.1 30.562 445 304 2 41 483 27 468 6.45e-61 208
Msa1412370 AT1G66780.1 32.791 430 277 4 68 494 59 479 5.72e-60 205
Msa1412370 AT1G15170.1 31.991 447 289 4 44 483 34 472 2.03e-59 204
Msa1412370 AT1G73700.1 30.335 478 317 7 24 494 7 475 2.53e-59 203
Msa1412370 AT2G04050.1 31.780 472 306 5 21 483 1 465 7.95e-59 202
Msa1412370 AT2G04100.2 30.000 440 303 2 41 478 27 463 2.61e-58 201
Msa1412370 AT2G04090.1 29.130 460 320 3 27 483 12 468 8.26e-58 199
Msa1412370 AT1G15180.1 31.707 451 293 4 40 483 31 473 3.57e-55 192
Msa1412370 AT2G34360.2 28.361 476 279 9 33 501 16 436 4.71e-55 191
Msa1412370 AT3G23550.1 30.612 441 299 5 43 480 30 466 5.42e-52 184
Msa1412370 AT1G66760.4 30.051 396 270 3 18 410 2 393 1.85e-51 180
Msa1412370 AT1G61890.1 28.228 457 311 6 44 493 47 493 7.43e-51 181
Msa1412370 AT1G11670.1 28.188 447 306 5 44 483 50 488 7.80e-51 181
Msa1412370 AT1G61890.2 28.054 442 303 5 44 478 47 480 8.16e-49 176
Msa1412370 AT1G15170.2 30.726 358 233 4 133 483 4 353 4.24e-47 167
Msa1412370 AT1G47530.1 27.872 470 326 4 19 483 11 472 7.35e-47 170
Msa1412370 AT3G26590.1 27.975 479 324 8 33 501 28 495 6.01e-46 168
Msa1412370 AT3G21690.1 29.350 477 321 5 24 495 40 505 6.46e-46 168
Msa1412370 AT3G23560.1 29.186 442 308 3 38 477 33 471 7.71e-46 167
Msa1412370 AT1G61890.3 28.842 423 292 5 44 462 47 464 1.24e-45 166
Msa1412370 AT1G15150.2 28.912 377 251 4 133 502 4 370 4.66e-45 162
Msa1412370 AT4G21910.3 26.923 442 317 4 44 483 55 492 3.32e-44 163
Msa1412370 AT4G21910.1 26.923 442 317 4 44 483 55 492 3.32e-44 163
Msa1412370 AT1G12950.1 28.118 473 306 12 43 498 62 517 3.26e-43 160
Msa1412370 AT4G21910.2 27.088 443 315 5 44 483 57 494 6.76e-43 160
Msa1412370 AT4G21910.4 27.088 443 315 5 44 483 57 494 6.76e-43 160
Msa1412370 AT5G38030.1 27.292 469 328 5 24 483 18 482 1.04e-41 156
Msa1412370 AT3G26590.2 28.321 399 275 4 105 501 4 393 8.65e-41 151
Msa1412370 AT4G00350.1 24.440 491 341 5 11 483 46 524 1.10e-40 154
Msa1412370 AT4G21903.1 26.812 414 297 4 72 483 81 490 8.55e-40 151
Msa1412370 AT5G10420.1 28.540 452 305 6 54 499 48 487 8.95e-40 150
Msa1412370 AT5G44050.1 28.918 453 307 7 46 493 46 488 4.13e-39 149
Msa1412370 AT1G23300.1 26.793 474 323 8 51 510 51 514 2.03e-38 147
Msa1412370 AT4G21903.2 26.506 415 299 4 72 484 81 491 4.16e-38 146
Msa1412370 AT3G23560.2 30.058 346 237 3 38 381 33 375 1.37e-37 143
Msa1412370 AT4G21903.3 27.034 381 273 3 105 483 4 381 6.05e-37 141
Msa1412370 AT3G59030.1 24.658 511 366 8 1 501 1 502 9.42e-37 142
Msa1412370 AT5G65380.1 27.273 462 323 6 28 483 21 475 2.53e-36 141
Msa1412370 AT1G47530.2 27.604 384 266 3 105 483 4 380 1.48e-35 137
Msa1412370 AT4G25640.1 25.630 476 331 5 21 481 1 468 2.50e-34 135
Msa1412370 AT4G25640.2 25.630 476 331 5 21 481 1 468 3.12e-34 135
Msa1412370 AT1G33100.1 27.982 436 304 5 51 482 40 469 6.29e-34 134
Msa1412370 AT1G33110.1 28.959 442 292 6 51 482 43 472 3.66e-33 132
Msa1412370 AT1G33090.1 27.412 456 305 7 41 482 29 472 6.29e-33 131
Msa1412370 AT1G66760.3 27.893 337 236 3 145 478 1 333 2.32e-32 127
Msa1412370 AT4G25640.3 25.450 444 308 5 21 449 1 436 3.91e-31 126
Msa1412370 AT5G17700.2 25.758 462 312 9 43 492 33 475 4.31e-31 126
Msa1412370 AT5G17700.1 25.758 462 312 9 43 492 33 475 4.31e-31 126
Msa1412370 AT3G03620.2 26.190 462 323 7 28 481 21 472 5.75e-31 126
Msa1412370 AT3G03620.1 26.190 462 323 7 28 481 21 472 5.75e-31 126
Msa1412370 AT1G15180.2 29.779 272 176 4 133 397 4 267 8.60e-30 121
Msa1412370 AT1G33080.2 26.126 444 310 6 51 486 43 476 4.57e-29 120
Msa1412370 AT1G33080.1 25.909 440 308 6 51 482 43 472 2.46e-28 118
Msa1412370 AT3G59030.2 24.515 412 297 7 1 402 1 408 1.92e-23 103
Msa1412370 AT1G33110.2 30.055 366 226 8 51 402 43 392 2.26e-23 102
Msa1412370 AT5G17700.3 26.075 372 249 8 43 402 33 390 5.11e-21 95.5

Find 124 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGCTGCAAGGGGAATTT+GGG 0.166621 tig0023889:+63952 Msa1412370:CDS
CTCTTCTTTGGACTTCTTAC+TGG 0.194547 tig0023889:+64202 Msa1412370:CDS
GGCCTTGTTGGACTCTTCTT+TGG 0.195245 tig0023889:+64190 Msa1412370:CDS
CTTCCTACCATGTTTAGTTC+TGG 0.202562 tig0023889:+63434 Msa1412370:CDS
TTCACTTTCGCTAACGTTAC+TGG 0.209280 tig0023889:+63113 Msa1412370:CDS
TGGTGGCTGCAAGGGGAATT+TGG 0.224291 tig0023889:+63951 Msa1412370:CDS
TCTTATTGTTGTTGTTCTTC+TGG 0.235360 tig0023889:+63547 Msa1412370:CDS
AGCTTAGCCGGTGGCGCGCT+CGG 0.247230 tig0023889:+63089 Msa1412370:CDS
ATGTCATCAACATGCAAATT+AGG 0.269692 tig0023889:+62852 Msa1412370:CDS
GAAATTCTAGTCTTGCTTAC+TGG 0.273403 tig0023889:+63716 Msa1412370:CDS
CTCTAGAATTAAAACATAAA+TGG 0.277546 tig0023889:-63570 None:intergenic
TTCCTACCATGTTTAGTTCT+GGG 0.312277 tig0023889:+63435 Msa1412370:CDS
TGAAGCCACATGAAAGTAAT+AGG 0.326554 tig0023889:-63266 None:intergenic
CATGATAGGGGCACTATAAA+TGG 0.328539 tig0023889:+63989 Msa1412370:CDS
AAGCTTCGTCTTGGCCTTGT+TGG 0.336381 tig0023889:+64178 Msa1412370:CDS
CAAGAAATTTCCACTGTTGC+TGG 0.340485 tig0023889:+63323 Msa1412370:CDS
TTTGAGAGTAGTATGTTAAC+AGG 0.351355 tig0023889:-63479 None:intergenic
CCATGAGCCTGTCCACAAAT+AGG 0.351874 tig0023889:-63173 None:intergenic
CACAGGGAACTTCTTTAACT+TGG 0.357675 tig0023889:-64318 None:intergenic
GACAATTGTATTGTTGCTAT+TGG 0.359414 tig0023889:+63235 Msa1412370:CDS
GCTTAGCCGGTGGCGCGCTC+GGG 0.363500 tig0023889:+63090 Msa1412370:CDS
AGAGTATTATGGATTGGATT+AGG 0.376619 tig0023889:+63636 Msa1412370:CDS
TGCTGTTGTGATTGCTGTCT+TGG 0.380543 tig0023889:-63042 None:intergenic
CAGTGGAAATTTCTTGTTGT+TGG 0.381992 tig0023889:-63316 None:intergenic
TTTCTCAGTGATGCTGTCAT+TGG 0.384106 tig0023889:+63805 Msa1412370:CDS
GAGGGGTTTCCATGGCTGTT+TGG 0.394339 tig0023889:+63516 Msa1412370:CDS
ATTAGGCTAATCAAGCTATC+TGG 0.398009 tig0023889:+63653 Msa1412370:CDS
GGCAACTCTTCAGCCATGAT+AGG 0.398212 tig0023889:+63975 Msa1412370:CDS
GTGGCTGCAAGGGGAATTTG+GGG 0.399284 tig0023889:+63953 Msa1412370:CDS
TCAGAGGAGCTAAGAGTTCA+AGG 0.400477 tig0023889:+62975 Msa1412370:CDS
ACTGGCTTCTCTGTCTTGAA+TGG 0.406137 tig0023889:+63131 Msa1412370:CDS
CGCAGGTTGCACCGGCAGCT+TGG 0.414906 tig0023889:+63925 Msa1412370:CDS
GCTTGGTGATGGTGGCTGCA+AGG 0.424849 tig0023889:+63942 Msa1412370:CDS
CGCAAACGCCAAGCAAGCAT+TGG 0.427319 tig0023889:+63745 Msa1412370:CDS
CGCCAAGCTCGTTTGAGACA+CGG 0.430905 tig0023889:-63844 None:intergenic
CTTGGTGATGGTGGCTGCAA+GGG 0.432045 tig0023889:+63943 Msa1412370:CDS
TATTATTAGTATTCATTGCT+AGG 0.432752 tig0023889:+64245 Msa1412370:CDS
GCACGTGTGTCTCTAGGAAT+AGG 0.444169 tig0023889:+63896 Msa1412370:CDS
AGGGACAGCTCGACCTTGGT+TGG 0.445218 tig0023889:+64090 Msa1412370:CDS
TGGTACTTCTGCGTCATCAC+AGG 0.449230 tig0023889:-64335 None:intergenic
TTTGCCTTCAAGCTTCGTCT+TGG 0.451622 tig0023889:+64169 Msa1412370:CDS
CATTTATAGTGCCCCTATCA+TGG 0.457544 tig0023889:-63988 None:intergenic
ACCGGTACGCTCGGTCGGCT+TGG 0.459703 tig0023889:-63871 None:intergenic
TAAAACTCCCAATGCTTGCT+TGG 0.462126 tig0023889:-63753 None:intergenic
TCTAGAGAATCGAAAGGTAT+TGG 0.462933 tig0023889:+63586 Msa1412370:CDS
ACTTGCTAATATTTGTGCCT+TGG 0.463290 tig0023889:-64290 None:intergenic
TCTGAAAGCAGCGTCTTCGC+TGG 0.467091 tig0023889:-62957 None:intergenic
TTGGGTATGTATGCAAATCT+AGG 0.477841 tig0023889:+64109 Msa1412370:CDS
CGTGGAAAGAAGGAGGATGG+TGG 0.478669 tig0023889:+63609 Msa1412370:CDS
ATGGTGGGATCAGAGTATTA+TGG 0.479182 tig0023889:+63625 Msa1412370:CDS
CTAGGTGAGCTCAGCTTAGC+CGG 0.481845 tig0023889:+63077 Msa1412370:CDS
GCTAAATTCATCAACACCAT+TGG 0.482192 tig0023889:-63011 None:intergenic
GCAACTCTTCAGCCATGATA+GGG 0.483823 tig0023889:+63976 Msa1412370:CDS
AGTGACATAACAAACAAATC+AGG 0.486224 tig0023889:-63368 None:intergenic
GAATGGTCTCTGTGGTGCCA+TGG 0.486890 tig0023889:+63148 Msa1412370:CDS
TTGTTGTCTCAACACATGTA+TGG 0.488430 tig0023889:+63679 Msa1412370:CDS
GGGACAGCTCGACCTTGGTT+GGG 0.495004 tig0023889:+64091 Msa1412370:CDS
GGGATCAGAGTATTATGGAT+TGG 0.495416 tig0023889:+63630 Msa1412370:CDS
TCCTACCATGTTTAGTTCTG+GGG 0.499436 tig0023889:+63436 Msa1412370:CDS
ACCCGTGTCTCAAACGAGCT+TGG 0.500843 tig0023889:+63842 Msa1412370:CDS
CCTATTTGTGGACAGGCTCA+TGG 0.501376 tig0023889:+63173 Msa1412370:CDS
TATTCATTGCTAGGATAAAG+TGG 0.503924 tig0023889:+64254 Msa1412370:CDS
CGGTCAGCACGTGTGTCTCT+AGG 0.507994 tig0023889:+63890 Msa1412370:CDS
AAGGCCAAGACGAAGCTTGA+AGG 0.508427 tig0023889:-64173 None:intergenic
GGGTCTAAGAGGGGTTTCCA+TGG 0.510224 tig0023889:+63508 Msa1412370:CDS
GCCAAGCTCGTTTGAGACAC+GGG 0.510977 tig0023889:-63843 None:intergenic
AGTGAACCCGAGCGCGCCAC+CGG 0.515331 tig0023889:-63096 None:intergenic
AAGGTATTGGCGTGGAAAGA+AGG 0.518806 tig0023889:+63599 Msa1412370:CDS
GCAAACGCCAAGCAAGCATT+GGG 0.519010 tig0023889:+63746 Msa1412370:CDS
AGCCACCATCACCAAGCTGC+CGG 0.520002 tig0023889:-63936 None:intergenic
CGGGTCTGTAATGGTGGAGG+AGG 0.520121 tig0023889:-62918 None:intergenic
CATGGAACCTATTTGTGGAC+AGG 0.523239 tig0023889:+63166 Msa1412370:CDS
TTGCACCGGCAGCTTGGTGA+TGG 0.528564 tig0023889:+63931 Msa1412370:CDS
GTGGAGGAGGTGACATTGGT+TGG 0.531112 tig0023889:-62905 None:intergenic
TTCGAGGGACAGCTCGACCT+TGG 0.533695 tig0023889:+64086 Msa1412370:CDS
CACCGGCAGCTTGGTGATGG+TGG 0.540745 tig0023889:+63934 Msa1412370:CDS
AGCGAAGACGCTGCTTTCAG+AGG 0.548362 tig0023889:+62959 Msa1412370:CDS
GTTTGTGGAGGCATAGTTCG+AGG 0.549958 tig0023889:+64070 Msa1412370:CDS
ATGCCTCCACAAACTGCCAA+TGG 0.550253 tig0023889:-64061 None:intergenic
ATCAGTTATCCAAACAGCCA+TGG 0.552397 tig0023889:-63525 None:intergenic
TGGCGTGGAAAGAAGGAGGA+TGG 0.552982 tig0023889:+63606 Msa1412370:CDS
CACGTGCTGACCGGTACGCT+CGG 0.555036 tig0023889:-63880 None:intergenic
CTAGAGACACACGTGCTGAC+CGG 0.558867 tig0023889:-63889 None:intergenic
AATGTTGTTGATGGCTCTAG+TGG 0.559949 tig0023889:+64024 Msa1412370:CDS
TTTCGGGTCTGTAATGGTGG+AGG 0.563765 tig0023889:-62921 None:intergenic
GTATGTATGCAAATCTAGGC+GGG 0.568375 tig0023889:+64113 Msa1412370:CDS
CTTGCTTGGCGTTTGCGAGG+TGG 0.568986 tig0023889:-63739 None:intergenic
GGTGCCATGGAACCTATTTG+TGG 0.570180 tig0023889:+63161 Msa1412370:CDS
GTGGAAAGAAGGAGGATGGT+GGG 0.570875 tig0023889:+63610 Msa1412370:CDS
CTGTCCACAAATAGGTTCCA+TGG 0.574641 tig0023889:-63165 None:intergenic
CAATTCCTATTACTTTCATG+TGG 0.579908 tig0023889:+63261 Msa1412370:CDS
ACCAAGCCGACCGAGCGTAC+CGG 0.581822 tig0023889:+63870 Msa1412370:CDS
GGAAACCCCTCTTAGACCCA+TGG 0.585351 tig0023889:-63504 None:intergenic
TTTCCATTGGCAGTTTGTGG+AGG 0.586502 tig0023889:+64058 Msa1412370:CDS
TGGCTGCAAGGGGAATTTGG+GGG 0.591531 tig0023889:+63954 Msa1412370:CDS
TGGTGTTGATGAATTTAGCT+TGG 0.591762 tig0023889:+63015 Msa1412370:CDS
AACATAAGTACCAGCAACAG+TGG 0.598946 tig0023889:-63333 None:intergenic
GTGGGTGGAAGAAGCTGCCA+AGG 0.602858 tig0023889:+64273 Msa1412370:CDS
TTGGTGATGGTGGCTGCAAG+GGG 0.610161 tig0023889:+63944 Msa1412370:CDS
ATGCTTGCTTGGCGTTTGCG+AGG 0.612886 tig0023889:-63742 None:intergenic
TGCTGACCGGTACGCTCGGT+CGG 0.613523 tig0023889:-63876 None:intergenic
TTTAATTCTAGAGAATCGAA+AGG 0.625448 tig0023889:+63580 Msa1412370:CDS
GGTACTTCTGCGTCATCACA+GGG 0.629425 tig0023889:-64334 None:intergenic
GTATTGGCGTGGAAAGAAGG+AGG 0.632332 tig0023889:+63602 Msa1412370:CDS
ATTCATTGCTAGGATAAAGT+GGG 0.633439 tig0023889:+64255 Msa1412370:CDS
CAACTCTTCAGCCATGATAG+GGG 0.633765 tig0023889:+63977 Msa1412370:CDS
AATGGTGGAGGAGGTGACAT+TGG 0.637951 tig0023889:-62909 None:intergenic
GGTGAGCTCAGCTTAGCCGG+TGG 0.638224 tig0023889:+63080 Msa1412370:CDS
TTTGTGGAGGCATAGTTCGA+GGG 0.638241 tig0023889:+64071 Msa1412370:CDS
GACACGGGTGGAAACACAAG+TGG 0.646609 tig0023889:-63828 None:intergenic
GTCCAAAGAAGAGTCCAACA+AGG 0.652628 tig0023889:-64192 None:intergenic
CATTGCTAGGATAAAGTGGG+TGG 0.653404 tig0023889:+64258 Msa1412370:CDS
TCTGTCTTGAATGGTCTCTG+TGG 0.655881 tig0023889:+63140 Msa1412370:CDS
AGAATCGAAAGGTATTGGCG+TGG 0.660271 tig0023889:+63591 Msa1412370:CDS
TCAACACATGTATGGAGTGG+TGG 0.666907 tig0023889:+63687 Msa1412370:CDS
TCATGAGAAGTGTCTTGTGA+AGG 0.670945 tig0023889:-63214 None:intergenic
TGCTACCCCAGAACTAAACA+TGG 0.676669 tig0023889:-63441 None:intergenic
ATAGTCAAAGTTCAACACAA+TGG 0.680940 tig0023889:-63777 None:intergenic
ACCCCAGAACTAAACATGGT+AGG 0.687373 tig0023889:-63437 None:intergenic
GTCTCAACACATGTATGGAG+TGG 0.690627 tig0023889:+63684 Msa1412370:CDS
AAGCTCGTTTGAGACACGGG+TGG 0.702539 tig0023889:-63840 None:intergenic
GGTATGTATGCAAATCTAGG+CGG 0.704766 tig0023889:+64112 Msa1412370:CDS
TGACGCAGAAGTACCAACTG+AGG 0.735542 tig0023889:+64342 Msa1412370:CDS
TTTGCATACATACCCAACCA+AGG 0.735927 tig0023889:-64103 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GACAAAATTTTGATTCATTT+TGG + tig0023889:63293-63312 Msa1412370:CDS 20.0%
!!! CTCTAGAATTAAAACATAAA+TGG - tig0023889:63573-63592 None:intergenic 20.0%
!! TATTATTAGTATTCATTGCT+AGG + tig0023889:64245-64264 Msa1412370:CDS 20.0%
!!! GACAAAATTTTGATTCATTT+TGG + tig0023889:63293-63312 Msa1412370:CDS 20.0%
!!! CTCTAGAATTAAAACATAAA+TGG - tig0023889:63573-63592 None:intergenic 20.0%
!! TATTATTAGTATTCATTGCT+AGG + tig0023889:64245-64264 Msa1412370:CDS 20.0%
!! TTTAATTCTAGAGAATCGAA+AGG + tig0023889:63580-63599 Msa1412370:CDS 25.0%
! AAAAAAGACAATGTTGTTGA+TGG + tig0023889:64015-64034 Msa1412370:CDS 25.0%
!! TTTAATTCTAGAGAATCGAA+AGG + tig0023889:63580-63599 Msa1412370:CDS 25.0%
! AAAAAAGACAATGTTGTTGA+TGG + tig0023889:64015-64034 Msa1412370:CDS 25.0%
ATGTCATCAACATGCAAATT+AGG + tig0023889:62852-62871 Msa1412370:CDS 30.0%
!!! TAGTTTTATGGTTTGAGCTA+AGG - tig0023889:62884-62903 None:intergenic 30.0%
!!! ACATTGGTTGGTTAGTTTTA+TGG - tig0023889:62896-62915 None:intergenic 30.0%
GACAATTGTATTGTTGCTAT+TGG + tig0023889:63235-63254 Msa1412370:CDS 30.0%
CAATTCCTATTACTTTCATG+TGG + tig0023889:63261-63280 Msa1412370:CDS 30.0%
AGTGACATAACAAACAAATC+AGG - tig0023889:63371-63390 None:intergenic 30.0%
TTTGAGAGTAGTATGTTAAC+AGG - tig0023889:63482-63501 None:intergenic 30.0%
ATACTACTCTCAAAAACCAT+GGG + tig0023889:63488-63507 Msa1412370:CDS 30.0%
! TCTTATTGTTGTTGTTCTTC+TGG + tig0023889:63547-63566 Msa1412370:CDS 30.0%
!! AGAGTATTATGGATTGGATT+AGG + tig0023889:63636-63655 Msa1412370:CDS 30.0%
ATAGTCAAAGTTCAACACAA+TGG - tig0023889:63780-63799 None:intergenic 30.0%
!! GGAGTTGTTTAATTTTCCAT+TGG + tig0023889:64045-64064 Msa1412370:CDS 30.0%
TATTCATTGCTAGGATAAAG+TGG + tig0023889:64254-64273 Msa1412370:CDS 30.0%
ATTCATTGCTAGGATAAAGT+GGG + tig0023889:64255-64274 Msa1412370:CDS 30.0%
ATGTCATCAACATGCAAATT+AGG + tig0023889:62852-62871 Msa1412370:CDS 30.0%
!!! TAGTTTTATGGTTTGAGCTA+AGG - tig0023889:62884-62903 None:intergenic 30.0%
!!! ACATTGGTTGGTTAGTTTTA+TGG - tig0023889:62896-62915 None:intergenic 30.0%
GACAATTGTATTGTTGCTAT+TGG + tig0023889:63235-63254 Msa1412370:CDS 30.0%
CAATTCCTATTACTTTCATG+TGG + tig0023889:63261-63280 Msa1412370:CDS 30.0%
AGTGACATAACAAACAAATC+AGG - tig0023889:63371-63390 None:intergenic 30.0%
TTTGAGAGTAGTATGTTAAC+AGG - tig0023889:63482-63501 None:intergenic 30.0%
ATACTACTCTCAAAAACCAT+GGG + tig0023889:63488-63507 Msa1412370:CDS 30.0%
! TCTTATTGTTGTTGTTCTTC+TGG + tig0023889:63547-63566 Msa1412370:CDS 30.0%
!! AGAGTATTATGGATTGGATT+AGG + tig0023889:63636-63655 Msa1412370:CDS 30.0%
ATAGTCAAAGTTCAACACAA+TGG - tig0023889:63780-63799 None:intergenic 30.0%
!! GGAGTTGTTTAATTTTCCAT+TGG + tig0023889:64045-64064 Msa1412370:CDS 30.0%
TATTCATTGCTAGGATAAAG+TGG + tig0023889:64254-64273 Msa1412370:CDS 30.0%
ATTCATTGCTAGGATAAAGT+GGG + tig0023889:64255-64274 Msa1412370:CDS 30.0%
!!! AGGAAGATTAGCTTTTCCAA+TGG + tig0023889:62995-63014 Msa1412370:CDS 35.0%
GCTAAATTCATCAACACCAT+TGG - tig0023889:63014-63033 None:intergenic 35.0%
!! TGGTGTTGATGAATTTAGCT+TGG + tig0023889:63015-63034 Msa1412370:CDS 35.0%
! AGCAATCACAACAGCATTTT+TGG + tig0023889:63049-63068 Msa1412370:CDS 35.0%
! GCAATCACAACAGCATTTTT+GGG + tig0023889:63050-63069 Msa1412370:CDS 35.0%
TGAAGCCACATGAAAGTAAT+AGG - tig0023889:63269-63288 None:intergenic 35.0%
! CAGTGGAAATTTCTTGTTGT+TGG - tig0023889:63319-63338 None:intergenic 35.0%
! GAGCTCAAGTAAGCTTTTAA+GGG - tig0023889:63401-63420 None:intergenic 35.0%
! AGAGCTCAAGTAAGCTTTTA+AGG - tig0023889:63402-63421 None:intergenic 35.0%
!! TTCCTACCATGTTTAGTTCT+GGG + tig0023889:63435-63454 Msa1412370:CDS 35.0%
CATACTACTCTCAAAAACCA+TGG + tig0023889:63487-63506 Msa1412370:CDS 35.0%
! TCTAGAGAATCGAAAGGTAT+TGG + tig0023889:63586-63605 Msa1412370:CDS 35.0%
ATTAGGCTAATCAAGCTATC+TGG + tig0023889:63653-63672 Msa1412370:CDS 35.0%
TTGTTGTCTCAACACATGTA+TGG + tig0023889:63679-63698 Msa1412370:CDS 35.0%
!! GAAATTCTAGTCTTGCTTAC+TGG + tig0023889:63716-63735 Msa1412370:CDS 35.0%
! AATTTTCCATTGGCAGTTTG+TGG + tig0023889:64055-64074 Msa1412370:CDS 35.0%
TTGGGTATGTATGCAAATCT+AGG + tig0023889:64109-64128 Msa1412370:CDS 35.0%
! ACTTGCTAATATTTGTGCCT+TGG - tig0023889:64293-64312 None:intergenic 35.0%
!!! AGGAAGATTAGCTTTTCCAA+TGG + tig0023889:62995-63014 Msa1412370:CDS 35.0%
GCTAAATTCATCAACACCAT+TGG - tig0023889:63014-63033 None:intergenic 35.0%
!! TGGTGTTGATGAATTTAGCT+TGG + tig0023889:63015-63034 Msa1412370:CDS 35.0%
! AGCAATCACAACAGCATTTT+TGG + tig0023889:63049-63068 Msa1412370:CDS 35.0%
! GCAATCACAACAGCATTTTT+GGG + tig0023889:63050-63069 Msa1412370:CDS 35.0%
TGAAGCCACATGAAAGTAAT+AGG - tig0023889:63269-63288 None:intergenic 35.0%
! CAGTGGAAATTTCTTGTTGT+TGG - tig0023889:63319-63338 None:intergenic 35.0%
! GAGCTCAAGTAAGCTTTTAA+GGG - tig0023889:63401-63420 None:intergenic 35.0%
! AGAGCTCAAGTAAGCTTTTA+AGG - tig0023889:63402-63421 None:intergenic 35.0%
!! TTCCTACCATGTTTAGTTCT+GGG + tig0023889:63435-63454 Msa1412370:CDS 35.0%
CATACTACTCTCAAAAACCA+TGG + tig0023889:63487-63506 Msa1412370:CDS 35.0%
! TCTAGAGAATCGAAAGGTAT+TGG + tig0023889:63586-63605 Msa1412370:CDS 35.0%
ATTAGGCTAATCAAGCTATC+TGG + tig0023889:63653-63672 Msa1412370:CDS 35.0%
TTGTTGTCTCAACACATGTA+TGG + tig0023889:63679-63698 Msa1412370:CDS 35.0%
!! GAAATTCTAGTCTTGCTTAC+TGG + tig0023889:63716-63735 Msa1412370:CDS 35.0%
! AATTTTCCATTGGCAGTTTG+TGG + tig0023889:64055-64074 Msa1412370:CDS 35.0%
TTGGGTATGTATGCAAATCT+AGG + tig0023889:64109-64128 Msa1412370:CDS 35.0%
! ACTTGCTAATATTTGTGCCT+TGG - tig0023889:64293-64312 None:intergenic 35.0%
!!! TCACATTTTCGGGTCTGTAA+TGG - tig0023889:62930-62949 None:intergenic 40.0%
! TGGATTTGCGTCACATTTTC+GGG - tig0023889:62940-62959 None:intergenic 40.0%
! CTGGATTTGCGTCACATTTT+CGG - tig0023889:62941-62960 None:intergenic 40.0%
TTCACTTTCGCTAACGTTAC+TGG + tig0023889:63113-63132 Msa1412370:CDS 40.0%
!! TCATGAGAAGTGTCTTGTGA+AGG - tig0023889:63217-63236 None:intergenic 40.0%
CAAGAAATTTCCACTGTTGC+TGG + tig0023889:63323-63342 Msa1412370:CDS 40.0%
AACATAAGTACCAGCAACAG+TGG - tig0023889:63336-63355 None:intergenic 40.0%
! CTTCCTACCATGTTTAGTTC+TGG + tig0023889:63434-63453 Msa1412370:CDS 40.0%
!! TCCTACCATGTTTAGTTCTG+GGG + tig0023889:63436-63455 Msa1412370:CDS 40.0%
TCAAAAACCATGGGTCTAAG+AGG + tig0023889:63497-63516 Msa1412370:CDS 40.0%
CAAAAACCATGGGTCTAAGA+GGG + tig0023889:63498-63517 Msa1412370:CDS 40.0%
AAAAACCATGGGTCTAAGAG+GGG + tig0023889:63499-63518 Msa1412370:CDS 40.0%
ATCAGTTATCCAAACAGCCA+TGG - tig0023889:63528-63547 None:intergenic 40.0%
! ATGGTGGGATCAGAGTATTA+TGG + tig0023889:63625-63644 Msa1412370:CDS 40.0%
! GGGATCAGAGTATTATGGAT+TGG + tig0023889:63630-63649 Msa1412370:CDS 40.0%
TAAAACTCCCAATGCTTGCT+TGG - tig0023889:63756-63775 None:intergenic 40.0%
TTTCTCAGTGATGCTGTCAT+TGG + tig0023889:63805-63824 Msa1412370:CDS 40.0%
!! CTAGGAATAGGTTTTATCGC+AGG + tig0023889:63908-63927 Msa1412370:CDS 40.0%
CATTTATAGTGCCCCTATCA+TGG - tig0023889:63991-64010 None:intergenic 40.0%
!! CATGATAGGGGCACTATAAA+TGG + tig0023889:63989-64008 Msa1412370:CDS 40.0%
!! AATGTTGTTGATGGCTCTAG+TGG + tig0023889:64024-64043 Msa1412370:CDS 40.0%
TTTGCATACATACCCAACCA+AGG - tig0023889:64106-64125 None:intergenic 40.0%
GGTATGTATGCAAATCTAGG+CGG + tig0023889:64112-64131 Msa1412370:CDS 40.0%
GTATGTATGCAAATCTAGGC+GGG + tig0023889:64113-64132 Msa1412370:CDS 40.0%
!!! TCTAGGCGGGTTTTATTTTC+TGG + tig0023889:64126-64145 Msa1412370:CDS 40.0%
AAGGCAAAAACAACACCAAG+GGG - tig0023889:64157-64176 None:intergenic 40.0%
GAAGGCAAAAACAACACCAA+GGG - tig0023889:64158-64177 None:intergenic 40.0%
TGAAGGCAAAAACAACACCA+AGG - tig0023889:64159-64178 None:intergenic 40.0%
CTCTTCTTTGGACTTCTTAC+TGG + tig0023889:64202-64221 Msa1412370:CDS 40.0%
CACAGGGAACTTCTTTAACT+TGG - tig0023889:64321-64340 None:intergenic 40.0%
!!! TCACATTTTCGGGTCTGTAA+TGG - tig0023889:62930-62949 None:intergenic 40.0%
! TGGATTTGCGTCACATTTTC+GGG - tig0023889:62940-62959 None:intergenic 40.0%
! CTGGATTTGCGTCACATTTT+CGG - tig0023889:62941-62960 None:intergenic 40.0%
TTCACTTTCGCTAACGTTAC+TGG + tig0023889:63113-63132 Msa1412370:CDS 40.0%
!! TCATGAGAAGTGTCTTGTGA+AGG - tig0023889:63217-63236 None:intergenic 40.0%
CAAGAAATTTCCACTGTTGC+TGG + tig0023889:63323-63342 Msa1412370:CDS 40.0%
AACATAAGTACCAGCAACAG+TGG - tig0023889:63336-63355 None:intergenic 40.0%
! CTTCCTACCATGTTTAGTTC+TGG + tig0023889:63434-63453 Msa1412370:CDS 40.0%
!! TCCTACCATGTTTAGTTCTG+GGG + tig0023889:63436-63455 Msa1412370:CDS 40.0%
TCAAAAACCATGGGTCTAAG+AGG + tig0023889:63497-63516 Msa1412370:CDS 40.0%
CAAAAACCATGGGTCTAAGA+GGG + tig0023889:63498-63517 Msa1412370:CDS 40.0%
AAAAACCATGGGTCTAAGAG+GGG + tig0023889:63499-63518 Msa1412370:CDS 40.0%
ATCAGTTATCCAAACAGCCA+TGG - tig0023889:63528-63547 None:intergenic 40.0%
! ATGGTGGGATCAGAGTATTA+TGG + tig0023889:63625-63644 Msa1412370:CDS 40.0%
! GGGATCAGAGTATTATGGAT+TGG + tig0023889:63630-63649 Msa1412370:CDS 40.0%
TAAAACTCCCAATGCTTGCT+TGG - tig0023889:63756-63775 None:intergenic 40.0%
TTTCTCAGTGATGCTGTCAT+TGG + tig0023889:63805-63824 Msa1412370:CDS 40.0%
!! CTAGGAATAGGTTTTATCGC+AGG + tig0023889:63908-63927 Msa1412370:CDS 40.0%
CATTTATAGTGCCCCTATCA+TGG - tig0023889:63991-64010 None:intergenic 40.0%
!! CATGATAGGGGCACTATAAA+TGG + tig0023889:63989-64008 Msa1412370:CDS 40.0%
!! AATGTTGTTGATGGCTCTAG+TGG + tig0023889:64024-64043 Msa1412370:CDS 40.0%
TTTGCATACATACCCAACCA+AGG - tig0023889:64106-64125 None:intergenic 40.0%
GGTATGTATGCAAATCTAGG+CGG + tig0023889:64112-64131 Msa1412370:CDS 40.0%
GTATGTATGCAAATCTAGGC+GGG + tig0023889:64113-64132 Msa1412370:CDS 40.0%
!!! TCTAGGCGGGTTTTATTTTC+TGG + tig0023889:64126-64145 Msa1412370:CDS 40.0%
AAGGCAAAAACAACACCAAG+GGG - tig0023889:64157-64176 None:intergenic 40.0%
GAAGGCAAAAACAACACCAA+GGG - tig0023889:64158-64177 None:intergenic 40.0%
TGAAGGCAAAAACAACACCA+AGG - tig0023889:64159-64178 None:intergenic 40.0%
CTCTTCTTTGGACTTCTTAC+TGG + tig0023889:64202-64221 Msa1412370:CDS 40.0%
CACAGGGAACTTCTTTAACT+TGG - tig0023889:64321-64340 None:intergenic 40.0%
!!! CATTTTCGGGTCTGTAATGG+TGG - tig0023889:62927-62946 None:intergenic 45.0%
TCAGAGGAGCTAAGAGTTCA+AGG + tig0023889:62975-62994 Msa1412370:CDS 45.0%
TGCTGTTGTGATTGCTGTCT+TGG - tig0023889:63045-63064 None:intergenic 45.0%
!!! ACAGCATTTTTGGGTCGACT+AGG + tig0023889:63059-63078 Msa1412370:CDS 45.0%
! ACTGGCTTCTCTGTCTTGAA+TGG + tig0023889:63131-63150 Msa1412370:CDS 45.0%
TCTGTCTTGAATGGTCTCTG+TGG + tig0023889:63140-63159 Msa1412370:CDS 45.0%
CTGTCCACAAATAGGTTCCA+TGG - tig0023889:63168-63187 None:intergenic 45.0%
CATGGAACCTATTTGTGGAC+AGG + tig0023889:63166-63185 Msa1412370:CDS 45.0%
ACCCCAGAACTAAACATGGT+AGG - tig0023889:63440-63459 None:intergenic 45.0%
TGCTACCCCAGAACTAAACA+TGG - tig0023889:63444-63463 None:intergenic 45.0%
!! AGAATCGAAAGGTATTGGCG+TGG + tig0023889:63591-63610 Msa1412370:CDS 45.0%
!! AAGGTATTGGCGTGGAAAGA+AGG + tig0023889:63599-63618 Msa1412370:CDS 45.0%
GTCTCAACACATGTATGGAG+TGG + tig0023889:63684-63703 Msa1412370:CDS 45.0%
TCAACACATGTATGGAGTGG+TGG + tig0023889:63687-63706 Msa1412370:CDS 45.0%
GCAACTCTTCAGCCATGATA+GGG + tig0023889:63976-63995 Msa1412370:CDS 45.0%
CAACTCTTCAGCCATGATAG+GGG + tig0023889:63977-63996 Msa1412370:CDS 45.0%
TTTCCATTGGCAGTTTGTGG+AGG + tig0023889:64058-64077 Msa1412370:CDS 45.0%
! TTTGTGGAGGCATAGTTCGA+GGG + tig0023889:64071-64090 Msa1412370:CDS 45.0%
TTTGCCTTCAAGCTTCGTCT+TGG + tig0023889:64169-64188 Msa1412370:CDS 45.0%
GTCCAAAGAAGAGTCCAACA+AGG - tig0023889:64195-64214 None:intergenic 45.0%
CATTGCTAGGATAAAGTGGG+TGG + tig0023889:64258-64277 Msa1412370:CDS 45.0%
! TCATTTTCACGAGCCTCAGT+TGG - tig0023889:64358-64377 None:intergenic 45.0%
!!! CATTTTCGGGTCTGTAATGG+TGG - tig0023889:62927-62946 None:intergenic 45.0%
TCAGAGGAGCTAAGAGTTCA+AGG + tig0023889:62975-62994 Msa1412370:CDS 45.0%
TGCTGTTGTGATTGCTGTCT+TGG - tig0023889:63045-63064 None:intergenic 45.0%
!!! ACAGCATTTTTGGGTCGACT+AGG + tig0023889:63059-63078 Msa1412370:CDS 45.0%
! ACTGGCTTCTCTGTCTTGAA+TGG + tig0023889:63131-63150 Msa1412370:CDS 45.0%
TCTGTCTTGAATGGTCTCTG+TGG + tig0023889:63140-63159 Msa1412370:CDS 45.0%
CTGTCCACAAATAGGTTCCA+TGG - tig0023889:63168-63187 None:intergenic 45.0%
CATGGAACCTATTTGTGGAC+AGG + tig0023889:63166-63185 Msa1412370:CDS 45.0%
ACCCCAGAACTAAACATGGT+AGG - tig0023889:63440-63459 None:intergenic 45.0%
TGCTACCCCAGAACTAAACA+TGG - tig0023889:63444-63463 None:intergenic 45.0%
!! AGAATCGAAAGGTATTGGCG+TGG + tig0023889:63591-63610 Msa1412370:CDS 45.0%
!! AAGGTATTGGCGTGGAAAGA+AGG + tig0023889:63599-63618 Msa1412370:CDS 45.0%
GTCTCAACACATGTATGGAG+TGG + tig0023889:63684-63703 Msa1412370:CDS 45.0%
TCAACACATGTATGGAGTGG+TGG + tig0023889:63687-63706 Msa1412370:CDS 45.0%
GCAACTCTTCAGCCATGATA+GGG + tig0023889:63976-63995 Msa1412370:CDS 45.0%
CAACTCTTCAGCCATGATAG+GGG + tig0023889:63977-63996 Msa1412370:CDS 45.0%
TTTCCATTGGCAGTTTGTGG+AGG + tig0023889:64058-64077 Msa1412370:CDS 45.0%
! TTTGTGGAGGCATAGTTCGA+GGG + tig0023889:64071-64090 Msa1412370:CDS 45.0%
TTTGCCTTCAAGCTTCGTCT+TGG + tig0023889:64169-64188 Msa1412370:CDS 45.0%
GTCCAAAGAAGAGTCCAACA+AGG - tig0023889:64195-64214 None:intergenic 45.0%
CATTGCTAGGATAAAGTGGG+TGG + tig0023889:64258-64277 Msa1412370:CDS 45.0%
! TCATTTTCACGAGCCTCAGT+TGG - tig0023889:64358-64377 None:intergenic 45.0%
! AATGGTGGAGGAGGTGACAT+TGG - tig0023889:62912-62931 None:intergenic 50.0%
! TTTCGGGTCTGTAATGGTGG+AGG - tig0023889:62924-62943 None:intergenic 50.0%
! GGTGCCATGGAACCTATTTG+TGG + tig0023889:63161-63180 Msa1412370:CDS 50.0%
CCATGAGCCTGTCCACAAAT+AGG - tig0023889:63176-63195 None:intergenic 50.0%
CCTATTTGTGGACAGGCTCA+TGG + tig0023889:63173-63192 Msa1412370:CDS 50.0%
! GTATTGGCGTGGAAAGAAGG+AGG + tig0023889:63602-63621 Msa1412370:CDS 50.0%
GTGGAAAGAAGGAGGATGGT+GGG + tig0023889:63610-63629 Msa1412370:CDS 50.0%
GCAAACGCCAAGCAAGCATT+GGG + tig0023889:63746-63765 Msa1412370:CDS 50.0%
! GCACGTGTGTCTCTAGGAAT+AGG + tig0023889:63896-63915 Msa1412370:CDS 50.0%
!! GGTTTTATCGCAGGTTGCAC+CGG + tig0023889:63917-63936 Msa1412370:CDS 50.0%
GGCAACTCTTCAGCCATGAT+AGG + tig0023889:63975-63994 Msa1412370:CDS 50.0%
ATGCCTCCACAAACTGCCAA+TGG - tig0023889:64064-64083 None:intergenic 50.0%
! GTTTGTGGAGGCATAGTTCG+AGG + tig0023889:64070-64089 Msa1412370:CDS 50.0%
!!! TATTTTCTGGCTCTGCCCCT+TGG + tig0023889:64139-64158 Msa1412370:CDS 50.0%
AAGGCCAAGACGAAGCTTGA+AGG - tig0023889:64176-64195 None:intergenic 50.0%
!! AAGCTTCGTCTTGGCCTTGT+TGG + tig0023889:64178-64197 Msa1412370:CDS 50.0%
!! GGCCTTGTTGGACTCTTCTT+TGG + tig0023889:64190-64209 Msa1412370:CDS 50.0%
GGTACTTCTGCGTCATCACA+GGG - tig0023889:64337-64356 None:intergenic 50.0%
TGGTACTTCTGCGTCATCAC+AGG - tig0023889:64338-64357 None:intergenic 50.0%
!! TGACGCAGAAGTACCAACTG+AGG + tig0023889:64342-64361 Msa1412370:CDS 50.0%
! AATGGTGGAGGAGGTGACAT+TGG - tig0023889:62912-62931 None:intergenic 50.0%
! TTTCGGGTCTGTAATGGTGG+AGG - tig0023889:62924-62943 None:intergenic 50.0%
! GGTGCCATGGAACCTATTTG+TGG + tig0023889:63161-63180 Msa1412370:CDS 50.0%
CCATGAGCCTGTCCACAAAT+AGG - tig0023889:63176-63195 None:intergenic 50.0%
CCTATTTGTGGACAGGCTCA+TGG + tig0023889:63173-63192 Msa1412370:CDS 50.0%
! GTATTGGCGTGGAAAGAAGG+AGG + tig0023889:63602-63621 Msa1412370:CDS 50.0%
GTGGAAAGAAGGAGGATGGT+GGG + tig0023889:63610-63629 Msa1412370:CDS 50.0%
GCAAACGCCAAGCAAGCATT+GGG + tig0023889:63746-63765 Msa1412370:CDS 50.0%
! GCACGTGTGTCTCTAGGAAT+AGG + tig0023889:63896-63915 Msa1412370:CDS 50.0%
!! GGTTTTATCGCAGGTTGCAC+CGG + tig0023889:63917-63936 Msa1412370:CDS 50.0%
GGCAACTCTTCAGCCATGAT+AGG + tig0023889:63975-63994 Msa1412370:CDS 50.0%
ATGCCTCCACAAACTGCCAA+TGG - tig0023889:64064-64083 None:intergenic 50.0%
! GTTTGTGGAGGCATAGTTCG+AGG + tig0023889:64070-64089 Msa1412370:CDS 50.0%
!!! TATTTTCTGGCTCTGCCCCT+TGG + tig0023889:64139-64158 Msa1412370:CDS 50.0%
AAGGCCAAGACGAAGCTTGA+AGG - tig0023889:64176-64195 None:intergenic 50.0%
!! AAGCTTCGTCTTGGCCTTGT+TGG + tig0023889:64178-64197 Msa1412370:CDS 50.0%
!! GGCCTTGTTGGACTCTTCTT+TGG + tig0023889:64190-64209 Msa1412370:CDS 50.0%
GGTACTTCTGCGTCATCACA+GGG - tig0023889:64337-64356 None:intergenic 50.0%
TGGTACTTCTGCGTCATCAC+AGG - tig0023889:64338-64357 None:intergenic 50.0%
!! TGACGCAGAAGTACCAACTG+AGG + tig0023889:64342-64361 Msa1412370:CDS 50.0%
GTGGAGGAGGTGACATTGGT+TGG - tig0023889:62908-62927 None:intergenic 55.0%
TCTGAAAGCAGCGTCTTCGC+TGG - tig0023889:62960-62979 None:intergenic 55.0%
! AGCGAAGACGCTGCTTTCAG+AGG + tig0023889:62959-62978 Msa1412370:CDS 55.0%
CTAGGTGAGCTCAGCTTAGC+CGG + tig0023889:63077-63096 Msa1412370:CDS 55.0%
! GAATGGTCTCTGTGGTGCCA+TGG + tig0023889:63148-63167 Msa1412370:CDS 55.0%
GGAAACCCCTCTTAGACCCA+TGG - tig0023889:63507-63526 None:intergenic 55.0%
GGGTCTAAGAGGGGTTTCCA+TGG + tig0023889:63508-63527 Msa1412370:CDS 55.0%
GAGGGGTTTCCATGGCTGTT+TGG + tig0023889:63516-63535 Msa1412370:CDS 55.0%
TGGCGTGGAAAGAAGGAGGA+TGG + tig0023889:63606-63625 Msa1412370:CDS 55.0%
CGTGGAAAGAAGGAGGATGG+TGG + tig0023889:63609-63628 Msa1412370:CDS 55.0%
ATGCTTGCTTGGCGTTTGCG+AGG - tig0023889:63745-63764 None:intergenic 55.0%
CGCAAACGCCAAGCAAGCAT+TGG + tig0023889:63745-63764 Msa1412370:CDS 55.0%
GACACGGGTGGAAACACAAG+TGG - tig0023889:63831-63850 None:intergenic 55.0%
! AAGCTCGTTTGAGACACGGG+TGG - tig0023889:63843-63862 None:intergenic 55.0%
ACCCGTGTCTCAAACGAGCT+TGG + tig0023889:63842-63861 Msa1412370:CDS 55.0%
! GCCAAGCTCGTTTGAGACAC+GGG - tig0023889:63846-63865 None:intergenic 55.0%
! CGCCAAGCTCGTTTGAGACA+CGG - tig0023889:63847-63866 None:intergenic 55.0%
! CTAGAGACACACGTGCTGAC+CGG - tig0023889:63892-63911 None:intergenic 55.0%
!! CTTGGTGATGGTGGCTGCAA+GGG + tig0023889:63943-63962 Msa1412370:CDS 55.0%
!! TTGGTGATGGTGGCTGCAAG+GGG + tig0023889:63944-63963 Msa1412370:CDS 55.0%
TGGTGGCTGCAAGGGGAATT+TGG + tig0023889:63951-63970 Msa1412370:CDS 55.0%
GGTGGCTGCAAGGGGAATTT+GGG + tig0023889:63952-63971 Msa1412370:CDS 55.0%
GTGGCTGCAAGGGGAATTTG+GGG + tig0023889:63953-63972 Msa1412370:CDS 55.0%
TGGCTGCAAGGGGAATTTGG+GGG + tig0023889:63954-63973 Msa1412370:CDS 55.0%
GTGGAGGAGGTGACATTGGT+TGG - tig0023889:62908-62927 None:intergenic 55.0%
TCTGAAAGCAGCGTCTTCGC+TGG - tig0023889:62960-62979 None:intergenic 55.0%
! AGCGAAGACGCTGCTTTCAG+AGG + tig0023889:62959-62978 Msa1412370:CDS 55.0%
CTAGGTGAGCTCAGCTTAGC+CGG + tig0023889:63077-63096 Msa1412370:CDS 55.0%
! GAATGGTCTCTGTGGTGCCA+TGG + tig0023889:63148-63167 Msa1412370:CDS 55.0%
GGAAACCCCTCTTAGACCCA+TGG - tig0023889:63507-63526 None:intergenic 55.0%
GGGTCTAAGAGGGGTTTCCA+TGG + tig0023889:63508-63527 Msa1412370:CDS 55.0%
GAGGGGTTTCCATGGCTGTT+TGG + tig0023889:63516-63535 Msa1412370:CDS 55.0%
TGGCGTGGAAAGAAGGAGGA+TGG + tig0023889:63606-63625 Msa1412370:CDS 55.0%
CGTGGAAAGAAGGAGGATGG+TGG + tig0023889:63609-63628 Msa1412370:CDS 55.0%
ATGCTTGCTTGGCGTTTGCG+AGG - tig0023889:63745-63764 None:intergenic 55.0%
CGCAAACGCCAAGCAAGCAT+TGG + tig0023889:63745-63764 Msa1412370:CDS 55.0%
GACACGGGTGGAAACACAAG+TGG - tig0023889:63831-63850 None:intergenic 55.0%
! AAGCTCGTTTGAGACACGGG+TGG - tig0023889:63843-63862 None:intergenic 55.0%
ACCCGTGTCTCAAACGAGCT+TGG + tig0023889:63842-63861 Msa1412370:CDS 55.0%
! GCCAAGCTCGTTTGAGACAC+GGG - tig0023889:63846-63865 None:intergenic 55.0%
! CGCCAAGCTCGTTTGAGACA+CGG - tig0023889:63847-63866 None:intergenic 55.0%
! CTAGAGACACACGTGCTGAC+CGG - tig0023889:63892-63911 None:intergenic 55.0%
!! CTTGGTGATGGTGGCTGCAA+GGG + tig0023889:63943-63962 Msa1412370:CDS 55.0%
!! TTGGTGATGGTGGCTGCAAG+GGG + tig0023889:63944-63963 Msa1412370:CDS 55.0%
TGGTGGCTGCAAGGGGAATT+TGG + tig0023889:63951-63970 Msa1412370:CDS 55.0%
GGTGGCTGCAAGGGGAATTT+GGG + tig0023889:63952-63971 Msa1412370:CDS 55.0%
GTGGCTGCAAGGGGAATTTG+GGG + tig0023889:63953-63972 Msa1412370:CDS 55.0%
TGGCTGCAAGGGGAATTTGG+GGG + tig0023889:63954-63973 Msa1412370:CDS 55.0%
! CGGGTCTGTAATGGTGGAGG+AGG - tig0023889:62921-62940 None:intergenic 60.0%
CTTGCTTGGCGTTTGCGAGG+TGG - tig0023889:63742-63761 None:intergenic 60.0%
CGGTCAGCACGTGTGTCTCT+AGG + tig0023889:63890-63909 Msa1412370:CDS 60.0%
! TTGCACCGGCAGCTTGGTGA+TGG + tig0023889:63931-63950 Msa1412370:CDS 60.0%
AGCCACCATCACCAAGCTGC+CGG - tig0023889:63939-63958 None:intergenic 60.0%
!! GCTTGGTGATGGTGGCTGCA+AGG + tig0023889:63942-63961 Msa1412370:CDS 60.0%
TTCGAGGGACAGCTCGACCT+TGG + tig0023889:64086-64105 Msa1412370:CDS 60.0%
AGGGACAGCTCGACCTTGGT+TGG + tig0023889:64090-64109 Msa1412370:CDS 60.0%
GGGACAGCTCGACCTTGGTT+GGG + tig0023889:64091-64110 Msa1412370:CDS 60.0%
GTGGGTGGAAGAAGCTGCCA+AGG + tig0023889:64273-64292 Msa1412370:CDS 60.0%
! CGGGTCTGTAATGGTGGAGG+AGG - tig0023889:62921-62940 None:intergenic 60.0%
CTTGCTTGGCGTTTGCGAGG+TGG - tig0023889:63742-63761 None:intergenic 60.0%
CGGTCAGCACGTGTGTCTCT+AGG + tig0023889:63890-63909 Msa1412370:CDS 60.0%
! TTGCACCGGCAGCTTGGTGA+TGG + tig0023889:63931-63950 Msa1412370:CDS 60.0%
AGCCACCATCACCAAGCTGC+CGG - tig0023889:63939-63958 None:intergenic 60.0%
!! GCTTGGTGATGGTGGCTGCA+AGG + tig0023889:63942-63961 Msa1412370:CDS 60.0%
TTCGAGGGACAGCTCGACCT+TGG + tig0023889:64086-64105 Msa1412370:CDS 60.0%
AGGGACAGCTCGACCTTGGT+TGG + tig0023889:64090-64109 Msa1412370:CDS 60.0%
GGGACAGCTCGACCTTGGTT+GGG + tig0023889:64091-64110 Msa1412370:CDS 60.0%
GTGGGTGGAAGAAGCTGCCA+AGG + tig0023889:64273-64292 Msa1412370:CDS 60.0%
GGTGAGCTCAGCTTAGCCGG+TGG + tig0023889:63080-63099 Msa1412370:CDS 65.0%
ACCAAGCCGACCGAGCGTAC+CGG + tig0023889:63870-63889 Msa1412370:CDS 65.0%
TGCTGACCGGTACGCTCGGT+CGG - tig0023889:63879-63898 None:intergenic 65.0%
! CACGTGCTGACCGGTACGCT+CGG - tig0023889:63883-63902 None:intergenic 65.0%
!! CACCGGCAGCTTGGTGATGG+TGG + tig0023889:63934-63953 Msa1412370:CDS 65.0%
GGTGAGCTCAGCTTAGCCGG+TGG + tig0023889:63080-63099 Msa1412370:CDS 65.0%
ACCAAGCCGACCGAGCGTAC+CGG + tig0023889:63870-63889 Msa1412370:CDS 65.0%
TGCTGACCGGTACGCTCGGT+CGG - tig0023889:63879-63898 None:intergenic 65.0%
! CACGTGCTGACCGGTACGCT+CGG - tig0023889:63883-63902 None:intergenic 65.0%
!! CACCGGCAGCTTGGTGATGG+TGG + tig0023889:63934-63953 Msa1412370:CDS 65.0%
AGCTTAGCCGGTGGCGCGCT+CGG + tig0023889:63089-63108 Msa1412370:CDS 70.0%
AGTGAACCCGAGCGCGCCAC+CGG - tig0023889:63099-63118 None:intergenic 70.0%
ACCGGTACGCTCGGTCGGCT+TGG - tig0023889:63874-63893 None:intergenic 70.0%
! CGCAGGTTGCACCGGCAGCT+TGG + tig0023889:63925-63944 Msa1412370:CDS 70.0%
AGCTTAGCCGGTGGCGCGCT+CGG + tig0023889:63089-63108 Msa1412370:CDS 70.0%
AGTGAACCCGAGCGCGCCAC+CGG - tig0023889:63099-63118 None:intergenic 70.0%
ACCGGTACGCTCGGTCGGCT+TGG - tig0023889:63874-63893 None:intergenic 70.0%
! CGCAGGTTGCACCGGCAGCT+TGG + tig0023889:63925-63944 Msa1412370:CDS 70.0%
GCTTAGCCGGTGGCGCGCTC+GGG + tig0023889:63090-63109 Msa1412370:CDS 75.0%
GCTTAGCCGGTGGCGCGCTC+GGG + tig0023889:63090-63109 Msa1412370:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0023889 gene 62852 64387 62852 ID=Msa1412370;Name=Msa1412370
tig0023889 mRNA 62852 64387 62852 ID=Msa1412370-mRNA-1;Parent=Msa1412370;Name=Msa1412370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|511
tig0023889 exon 62852 64387 62852 ID=Msa1412370-mRNA-1:exon:20537;Parent=Msa1412370-mRNA-1
tig0023889 CDS 62852 64387 62852 ID=Msa1412370-mRNA-1:cds;Parent=Msa1412370-mRNA-1
Gene Sequence

>Msa1412370

ATGTCATCAACATGCAAATTAGGAGAAAACCTTAGCTCAAACCATAAAACTAACCAACCAATGTCACCTCCTCCACCATTACAGACCCGAAAATGTGACGCAAATCCAGCGAAGACGCTGCTTTCAGAGGAGCTAAGAGTTCAAGGAAGATTAGCTTTTCCAATGGTGTTGATGAATTTAGCTTGGTTTGCCAAGACAGCAATCACAACAGCATTTTTGGGTCGACTAGGTGAGCTCAGCTTAGCCGGTGGCGCGCTCGGGTTCACTTTCGCTAACGTTACTGGCTTCTCTGTCTTGAATGGTCTCTGTGGTGCCATGGAACCTATTTGTGGACAGGCTCATGGAGCTAAAAACGTGAGACTCCTTCACAAGACACTTCTCATGACAATTGTATTGTTGCTATTGGTAACAATTCCTATTACTTTCATGTGGCTTCATATTGACAAAATTTTGATTCATTTTGGCCAACAACAAGAAATTTCCACTGTTGCTGGTACTTATGTTTACTATCTCATACCTGATTTGTTTGTTATGTCACTCTTGTGTCCCTTAAAAGCTTACTTGAGCTCTCAAAGCATCACTCTTCCTACCATGTTTAGTTCTGGGGTAGCACTTGCTTTTCATGTTCCTGTTAACATACTACTCTCAAAAACCATGGGTCTAAGAGGGGTTTCCATGGCTGTTTGGATAACTGATCTTATTGTTGTTGTTCTTCTGGCCATTTATGTTTTAATTCTAGAGAATCGAAAGGTATTGGCGTGGAAAGAAGGAGGATGGTGGGATCAGAGTATTATGGATTGGATTAGGCTAATCAAGCTATCTGGATCTTGTTGTCTCAACACATGTATGGAGTGGTGGTGCTATGAAATTCTAGTCTTGCTTACTGGCCACCTCGCAAACGCCAAGCAAGCATTGGGAGTTTTAGCCATTGTGTTGAACTTTGACTATTTACTTTTCTCAGTGATGCTGTCATTGGCCACTTGTGTTTCCACCCGTGTCTCAAACGAGCTTGGCGCAAACCAAGCCGACCGAGCGTACCGGTCAGCACGTGTGTCTCTAGGAATAGGTTTTATCGCAGGTTGCACCGGCAGCTTGGTGATGGTGGCTGCAAGGGGAATTTGGGGGCAACTCTTCAGCCATGATAGGGGCACTATAAATGGAGTAAAAAAGACAATGTTGTTGATGGCTCTAGTGGAGTTGTTTAATTTTCCATTGGCAGTTTGTGGAGGCATAGTTCGAGGGACAGCTCGACCTTGGTTGGGTATGTATGCAAATCTAGGCGGGTTTTATTTTCTGGCTCTGCCCCTTGGTGTTGTTTTTGCCTTCAAGCTTCGTCTTGGCCTTGTTGGACTCTTCTTTGGACTTCTTACTGGTATTGTTGTTTGCTTGTCATTATTATTAGTATTCATTGCTAGGATAAAGTGGGTGGAAGAAGCTGCCAAGGCACAAATATTAGCAAGTAACGACCAAGTTAAAGAAGTTCCCTGTGATGACGCAGAAGTACCAACTGAGGCTCGTGAAAATGACAAAGTGTAA
Protein sequence

>Msa1412370

MSSTCKLGENLSSNHKTNQPMSPPPPLQTRKCDANPAKTLLSEELRVQGRLAFPMVLMNLAWFAKTAITTAFLGRLGELSLAGGALGFTFANVTGFSVLNGLCGAMEPICGQAHGAKNVRLLHKTLLMTIVLLLLVTIPITFMWLHIDKILIHFGQQQEISTVAGTYVYYLIPDLFVMSLLCPLKAYLSSQSITLPTMFSSGVALAFHVPVNILLSKTMGLRGVSMAVWITDLIVVVLLAIYVLILENRKVLAWKEGGWWDQSIMDWIRLIKLSGSCCLNTCMEWWCYEILVLLTGHLANAKQALGVLAIVLNFDYLLFSVMLSLATCVSTRVSNELGANQADRAYRSARVSLGIGFIAGCTGSLVMVAARGIWGQLFSHDRGTINGVKKTMLLMALVELFNFPLAVCGGIVRGTARPWLGMYANLGGFYFLALPLGVVFAFKLRLGLVGLFFGLLTGIVVCLSLLLVFIARIKWVEEAAKAQILASNDQVKEVPCDDAEVPTEARENDKV*