AlfalfaGEDB | Msa0834920

M. sativa cultivar zhongmu-4 / Msa0834920

KEGG: K01869
GO: GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834920	KEH34036.1	76.522	115	4	1	1	92	1	115	1.02e-50	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834920	sp\|F4I116\|SYLC_ARATH	54.783	115	28	2	1	92	1	114	2.29e-33	125

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834920	A0A072UX73	76.522	115	4	1	1	92	1	115	4.87e-51	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0208090	Msa0834920	0.813398	2.612479e-51	-8.615850e-47
Msa0369460	Msa0834920	0.803415	3.523720e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834920	MtrunA17_Chr3g0101981	76.522	115	4	1	1	92	33	147	4.07e-53	178
Msa0834920	MtrunA17_Chr7g0214721	63.063	111	18	1	5	92	9	119	3.61e-41	144

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834920	AT1G09620.1	54.783	115	28	2	1	92	1	114	2.33e-34	125

Find 21 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGAGGCGGTTTGTTTC+CGG	0.204869	5_4:-8198271	None:intergenic
TAATCCTATTAAGGCTTCTT+TGG	0.213193	5_4:+8198434	Msa0834920:CDS
TTGTACTGGTATGCCTATTA+AGG	0.223988	5_4:+8198371	Msa0834920:CDS
GTTTCCAAAGAAGCCTTAAT+AGG	0.291760	5_4:-8198438	None:intergenic
TGTTGAGGCGGTTTGTTTCC+GGG	0.293644	5_4:-8198270	None:intergenic
TGTAAGTAACCGTTCATATA+AGG	0.331579	5_4:-8198324	None:intergenic
ACAGTTTGGTAATCCTATTA+AGG	0.335853	5_4:+8198425	Msa0834920:CDS
TTATCAGCAGAAGCCTTAAT+AGG	0.367730	5_4:-8198384	None:intergenic
TAACCGTTCATATAAGGAAA+AGG	0.416196	5_4:-8198318	None:intergenic
GCTAGGGAGATTCAACAGTT+TGG	0.421756	5_4:+8198411	Msa0834920:CDS
TCGAATCAAAAGTTCAGAAA+TGG	0.447842	5_4:+8198217	Msa0834920:CDS
ATTGTACTTGTATGATATTG+AGG	0.458271	5_4:+8198481	Msa0834920:CDS
AACCGTTCATATAAGGAAAA+GGG	0.486042	5_4:-8198317	None:intergenic
CTTCTGCTGATAAGCTTGCT+AGG	0.488309	5_4:+8198394	Msa0834920:CDS
ATGAACGGTTACTTACATCT+AGG	0.519049	5_4:+8198330	Msa0834920:CDS
AATCAAAAGTTCAGAAATGG+TGG	0.526513	5_4:+8198220	Msa0834920:CDS
TTCTGCTGATAAGCTTGCTA+GGG	0.564679	5_4:+8198395	Msa0834920:CDS
CAAGTCTTCAAATCTGAACC+CGG	0.581368	5_4:+8198252	Msa0834920:CDS
ATCAAAAGTTCAGAAATGGT+GGG	0.621795	5_4:+8198221	Msa0834920:CDS
AAGTTGCGGCGAAATGGCGT+CGG	0.657314	5_4:+8198152	None:intergenic
GGAAACAAACCGCCTCAACA+AGG	0.661763	5_4:+8198273	Msa0834920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTGTACTTGTATGATATTG+AGG	+	chr5_4:8198481-8198500	Msa0834920:CDS	25.0%
	TCGAATCAAAAGTTCAGAAA+TGG	+	chr5_4:8198217-8198236	Msa0834920:CDS	30.0%
	AATCAAAAGTTCAGAAATGG+TGG	+	chr5_4:8198220-8198239	Msa0834920:CDS	30.0%
	ATCAAAAGTTCAGAAATGGT+GGG	+	chr5_4:8198221-8198240	Msa0834920:CDS	30.0%
!	AAGAATTTTTCACCTTGTTG+AGG	-	chr5_4:8198288-8198307	None:intergenic	30.0%
	CAACAAGGTGAAAAATTCTT+TGG	+	chr5_4:8198288-8198307	Msa0834920:CDS	30.0%
!	TTCCCTTTTCCTTATATGAA+CGG	+	chr5_4:8198315-8198334	Msa0834920:CDS	30.0%
	AACCGTTCATATAAGGAAAA+GGG	-	chr5_4:8198320-8198339	None:intergenic	30.0%
	TAACCGTTCATATAAGGAAA+AGG	-	chr5_4:8198321-8198340	None:intergenic	30.0%
	TGTAAGTAACCGTTCATATA+AGG	-	chr5_4:8198327-8198346	None:intergenic	30.0%
!!!	GCTTTTGCTTTTTATTGTAC+TGG	+	chr5_4:8198357-8198376	Msa0834920:CDS	30.0%
!	ACAGTTTGGTAATCCTATTA+AGG	+	chr5_4:8198425-8198444	Msa0834920:CDS	30.0%
	TAATCCTATTAAGGCTTCTT+TGG	+	chr5_4:8198434-8198453	Msa0834920:CDS	30.0%
!	AATTTTTCACCTTGTTGAGG+CGG	-	chr5_4:8198285-8198304	None:intergenic	35.0%
	ATGAACGGTTACTTACATCT+AGG	+	chr5_4:8198330-8198349	Msa0834920:CDS	35.0%
!	TTGTACTGGTATGCCTATTA+AGG	+	chr5_4:8198371-8198390	Msa0834920:CDS	35.0%
	TTATCAGCAGAAGCCTTAAT+AGG	-	chr5_4:8198387-8198406	None:intergenic	35.0%
	GTTTCCAAAGAAGCCTTAAT+AGG	-	chr5_4:8198441-8198460	None:intergenic	35.0%
	CAAGTCTTCAAATCTGAACC+CGG	+	chr5_4:8198252-8198271	Msa0834920:CDS	40.0%
!!	TTCTGCTGATAAGCTTGCTA+GGG	+	chr5_4:8198395-8198414	Msa0834920:CDS	40.0%
!	TTGTTGAGGCGGTTTGTTTC+CGG	-	chr5_4:8198274-8198293	None:intergenic	45.0%
!	CTTCTGCTGATAAGCTTGCT+AGG	+	chr5_4:8198394-8198413	Msa0834920:CDS	45.0%
	GCTAGGGAGATTCAACAGTT+TGG	+	chr5_4:8198411-8198430	Msa0834920:CDS	45.0%
!	TGTTGAGGCGGTTTGTTTCC+GGG	-	chr5_4:8198273-8198292	None:intergenic	50.0%
	GGAAACAAACCGCCTCAACA+AGG	+	chr5_4:8198273-8198292	Msa0834920:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	8198165	8198509	8198165	ID=Msa0834920;Name=Msa0834920
chr5_4	mRNA	8198165	8198509	8198165	ID=Msa0834920-mRNA-1;Parent=Msa0834920;Name=Msa0834920-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|114
chr5_4	exon	8198165	8198509	8198165	ID=Msa0834920-mRNA-1:exon:3646;Parent=Msa0834920-mRNA-1
chr5_4	CDS	8198165	8198509	8198165	ID=Msa0834920-mRNA-1:cds;Parent=Msa0834920-mRNA-1

Gene Sequence	>Msa0834920 ATGGCGTCGGAAACTTCAAGAAGCTTCGTTCGTCACGATTGTCTTCGCGAAATCGAATCAAAAGTTCAGAAATGGTGGGAAGAGAAACAAGTCTTCAAATCTGAACCCGGAAACAAACCGCCTCAACAAGGTGAAAAATTCTTTGGAAATTTCCCTTTTCCTTATATGAACGGTTACTTACATCTAGGTCATGCTTTTGCTTTTTATTGTACTGGTATGCCTATTAAGGCTTCTGCTGATAAGCTTGCTAGGGAGATTCAACAGTTTGGTAATCCTATTAAGGCTTCTTTGGAAACAAGTCATGCTTTTGCTTTTCATTGTACTTGTATGATATTGAGGAATTAG
Protein sequence	>Msa0834920 MASETSRSFVRHDCLREIESKVQKWWEEKQVFKSEPGNKPPQQGEKFFGNFPFPYMNGYLHLGHAFAFYCTGMPIKASADKLAREIQQFGNPIKASLETSHAFAFHCTCMILRN*