AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0369460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTGGTTAGAAAGTTTA+AGG 0.124303 3_1:+66729905 Msa0369460:CDS
AGGAGCTAATCCAATAATTT+TGG 0.191379 3_1:+66730268 Msa0369460:three_prime_UTR
CAAATGTCAACCAAAATTAT+TGG 0.196726 3_1:-66730278 None:intergenic
ATTCATTCTATGAGGATTAA+TGG 0.239446 3_1:-66729320 None:intergenic
AATCTTGAAAGTGGAGAAAA+TGG 0.259600 3_1:-66730381 None:intergenic
GCAGAGCCTGTGCTCATCTT+TGG 0.268706 3_1:+66729890 Msa0369460:intron
GGAAAACAATAATGAATTAA+TGG 0.269127 3_1:-66730360 None:intergenic
ACAAGAAAGTCTAGTATGAT+AGG 0.295764 3_1:+66730227 Msa0369460:three_prime_UTR
CGTATGTGGTGAGTTTATTA+CGG 0.300587 3_1:+66729996 Msa0369460:three_prime_UTR
ACATTTGCGATCAGTTTATA+AGG 0.301304 3_1:-66729260 None:intergenic
CTGGTCCACAAGTCCAAGTT+AGG 0.321632 3_1:-66729728 None:intergenic
GACATTTGTTAGTTGATAAT+AGG 0.325990 3_1:+66730293 Msa0369460:three_prime_UTR
GCTCTGATGCTTCCTTGATA+TGG 0.339315 3_1:+66730081 Msa0369460:three_prime_UTR
CCTCATGTTGTGAGAAGAAT+TGG 0.348376 3_1:-66729358 None:intergenic
AATGTGAAACACTAAATTCA+TGG 0.390497 3_1:-66730144 None:intergenic
GAATGGAAATATGATTTAGC+TGG 0.426580 3_1:-66730422 None:intergenic
ATCCAAAATTGCAAAAGTAT+TGG 0.430002 3_1:-66729524 None:intergenic
GTATGTGGTGAGTTTATTAC+GGG 0.430987 3_1:+66729997 Msa0369460:three_prime_UTR
CTGCAGAATGTTCCATATCA+AGG 0.443308 3_1:-66730093 None:intergenic
CTATATTCAAAGTATCAATA+AGG 0.448283 3_1:+66729406 Msa0369460:five_prime_UTR
TAGGAGTATCATACATGTAT+TGG 0.458596 3_1:+66729491 Msa0369460:five_prime_UTR
TGCGATCAGTTTATAAGGAA+AGG 0.461040 3_1:-66729255 None:intergenic
TCTTTGGTTAGAAAGTTTAA+GGG 0.462562 3_1:+66729906 Msa0369460:CDS
TCAGAAGTTCACAGGAGAAT+AGG 0.466329 3_1:-66729952 None:intergenic
CGTAATAAACTCACCACATA+CGG 0.469466 3_1:-66729995 None:intergenic
GTTCCGTTATTCATTCTATG+AGG 0.472399 3_1:-66729328 None:intergenic
AATGGAAATATGATTTAGCT+GGG 0.473438 3_1:-66730421 None:intergenic
CTGTGTTCTCCTAGGCAAGC+TGG 0.481533 3_1:-66729747 None:intergenic
ATAAGGTATTCAAAATGTAT+TGG 0.483690 3_1:+66729423 Msa0369460:five_prime_UTR
CACTTCTTGGATATGTATCT+AGG 0.490643 3_1:+66729472 Msa0369460:five_prime_UTR
GCGATTGTGAAGGTTGAGAA+TGG 0.490798 3_1:-66729617 None:intergenic
GAAAGAAAACAAGGAGGATG+AGG 0.501972 3_1:+66730184 Msa0369460:three_prime_UTR
GGTGAATCAAATCTTGAAAG+TGG 0.504371 3_1:-66730390 None:intergenic
CGATTGTGAAGGTTGAGAAT+GGG 0.513942 3_1:-66729616 None:intergenic
AAGAATACCTGTGTTCTCCT+AGG 0.519834 3_1:-66729755 None:intergenic
AAGCATCAGAGCTGACCACT+AGG 0.527920 3_1:-66730070 None:intergenic
CAAGAAAGTCTAGTATGATA+GGG 0.531313 3_1:+66730228 Msa0369460:three_prime_UTR
AAGTTTAAGGGGCCATCTGA+AGG 0.533709 3_1:+66729918 Msa0369460:CDS
TATCATACATGTATTGGTAT+CGG 0.536685 3_1:+66729497 Msa0369460:five_prime_UTR
ATGAAATCTCAGAAGTTCAC+AGG 0.546895 3_1:-66729960 None:intergenic
ACTTGTGGACCAGCTTGCCT+AGG 0.547054 3_1:+66729738 Msa0369460:CDS
GTGCTCCGGTGTCCTTCAGA+TGG 0.553243 3_1:-66729930 None:intergenic
AATCCTCATAGAATGAATAA+CGG 0.555478 3_1:+66729325 Msa0369460:five_prime_UTR
TCACAGGAGAATAGGTGCTC+CGG 0.561752 3_1:-66729944 None:intergenic
AATTGATTCTTGATTACACT+CGG 0.563941 3_1:+66729551 Msa0369460:five_prime_UTR
TGGAAATATGATTTAGCTGG+GGG 0.580777 3_1:-66730419 None:intergenic
TTCTAACCAAAGATGAGCAC+AGG 0.581085 3_1:-66729896 None:intergenic
TGAAACACTAAATTCATGGA+AGG 0.589903 3_1:-66730140 None:intergenic
CTTTGGTTAGAAAGTTTAAG+GGG 0.593385 3_1:+66729907 Msa0369460:CDS
TGGTCCACAAGTCCAAGTTA+GGG 0.595970 3_1:-66729727 None:intergenic
ATGGAAATATGATTTAGCTG+GGG 0.599703 3_1:-66730420 None:intergenic
TCACAACATGAGGCAGAACA+AGG 0.600167 3_1:+66729368 Msa0369460:five_prime_UTR
TCATCCCTAACTTGGACTTG+TGG 0.612182 3_1:+66729723 Msa0369460:CDS
CTCATGTTGTGAGAAGAATT+GGG 0.615864 3_1:-66729357 None:intergenic
CCAATTCTTCTCACAACATG+AGG 0.620378 3_1:+66729358 Msa0369460:five_prime_UTR
AGCATCAGAGCTGACCACTA+GGG 0.629812 3_1:-66730069 None:intergenic
TATCTCTCAGAAAGAAAACA+AGG 0.631789 3_1:+66730175 Msa0369460:three_prime_UTR
AGGGGCCATCTGAAGGACAC+CGG 0.658208 3_1:+66729925 Msa0369460:CDS
CAGCTTGCCTAGGAGAACAC+AGG 0.688410 3_1:+66729748 Msa0369460:CDS
GGTACTATACAACACAAACA+CGG 0.692316 3_1:+66729075 None:intergenic
CTGCATTTCAGATACCCTAG+TGG 0.720877 3_1:+66730055 Msa0369460:three_prime_UTR
CTCTCAGAAAGAAAACAAGG+AGG 0.722961 3_1:+66730178 Msa0369460:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTACAATATATTTTGACAT+TGG - chr3_1:66729299-66729318 None:intergenic 15.0%
!!! ATTACAATATATTTTGACAT+TGG - chr3_1:66729299-66729318 None:intergenic 15.0%
!!! GAAAGGAATATTTTTGATAT+GGG - chr3_1:66729241-66729260 None:intergenic 20.0%
!!! CTATATTCAAAGTATCAATA+AGG + chr3_1:66729406-66729425 Msa0369460:five_prime_UTR 20.0%
!!! ATAAGGTATTCAAAATGTAT+TGG + chr3_1:66729423-66729442 Msa0369460:five_prime_UTR 20.0%
!!! GTCGATATTTTCTTAAATTT+TGG + chr3_1:66729445-66729464 Msa0369460:five_prime_UTR 20.0%
!!! GATATTTTCTTAAATTTTGG+AGG + chr3_1:66729448-66729467 Msa0369460:five_prime_UTR 20.0%
!! GGAAAACAATAATGAATTAA+TGG - chr3_1:66730363-66730382 None:intergenic 20.0%
!!! GAAAGGAATATTTTTGATAT+GGG - chr3_1:66729241-66729260 None:intergenic 20.0%
!!! CTATATTCAAAGTATCAATA+AGG + chr3_1:66729406-66729425 Msa0369460:five_prime_UTR 20.0%
!!! ATAAGGTATTCAAAATGTAT+TGG + chr3_1:66729423-66729442 Msa0369460:five_prime_UTR 20.0%
!!! GTCGATATTTTCTTAAATTT+TGG + chr3_1:66729445-66729464 Msa0369460:five_prime_UTR 20.0%
!!! GATATTTTCTTAAATTTTGG+AGG + chr3_1:66729448-66729467 Msa0369460:five_prime_UTR 20.0%
!! GGAAAACAATAATGAATTAA+TGG - chr3_1:66730363-66730382 None:intergenic 20.0%
!! AATTTTGCATTAGCATATCT+AGG - chr3_1:66729142-66729161 None:intergenic 25.0%
!!! GGAAAGGAATATTTTTGATA+TGG - chr3_1:66729242-66729261 None:intergenic 25.0%
! ATTCATTCTATGAGGATTAA+TGG - chr3_1:66729323-66729342 None:intergenic 25.0%
! AATCCTCATAGAATGAATAA+CGG + chr3_1:66729325-66729344 Msa0369460:five_prime_UTR 25.0%
!! TATCATACATGTATTGGTAT+CGG + chr3_1:66729497-66729516 Msa0369460:five_prime_UTR 25.0%
!! AACCAATACTTTTGCAATTT+TGG + chr3_1:66729522-66729541 Msa0369460:five_prime_UTR 25.0%
!! ATCCAAAATTGCAAAAGTAT+TGG - chr3_1:66729527-66729546 None:intergenic 25.0%
!! AATTGATTCTTGATTACACT+CGG + chr3_1:66729551-66729570 Msa0369460:five_prime_UTR 25.0%
!! AATCTTTTGTGGGATTTTTT+TGG + chr3_1:66729659-66729678 Msa0369460:CDS 25.0%
!!! CAATTTCGGTATTTTTAATC+TGG + chr3_1:66729792-66729811 Msa0369460:intron 25.0%
!!! TATTTTTAATCTGGAAACAC+AGG + chr3_1:66729801-66729820 Msa0369460:intron 25.0%
!!! AATGCATTTTTAAGAAGGAT+GGG + chr3_1:66729825-66729844 Msa0369460:intron 25.0%
! ATCTTTGGTTAGAAAGTTTA+AGG + chr3_1:66729905-66729924 Msa0369460:CDS 25.0%
! TCTTTGGTTAGAAAGTTTAA+GGG + chr3_1:66729906-66729925 Msa0369460:CDS 25.0%
!!! TGAGATTTCATTTTTGATTC+TGG + chr3_1:66729972-66729991 Msa0369460:three_prime_UTR 25.0%
! AATGTGAAACACTAAATTCA+TGG - chr3_1:66730147-66730166 None:intergenic 25.0%
! CAAATGTCAACCAAAATTAT+TGG - chr3_1:66730281-66730300 None:intergenic 25.0%
!!! GACATTTGTTAGTTGATAAT+AGG + chr3_1:66730293-66730312 Msa0369460:three_prime_UTR 25.0%
!! AATTTTGCATTAGCATATCT+AGG - chr3_1:66729142-66729161 None:intergenic 25.0%
!!! GGAAAGGAATATTTTTGATA+TGG - chr3_1:66729242-66729261 None:intergenic 25.0%
! ATTCATTCTATGAGGATTAA+TGG - chr3_1:66729323-66729342 None:intergenic 25.0%
! AATCCTCATAGAATGAATAA+CGG + chr3_1:66729325-66729344 Msa0369460:five_prime_UTR 25.0%
!! TATCATACATGTATTGGTAT+CGG + chr3_1:66729497-66729516 Msa0369460:five_prime_UTR 25.0%
!! AACCAATACTTTTGCAATTT+TGG + chr3_1:66729522-66729541 Msa0369460:five_prime_UTR 25.0%
!! ATCCAAAATTGCAAAAGTAT+TGG - chr3_1:66729527-66729546 None:intergenic 25.0%
!! AATTGATTCTTGATTACACT+CGG + chr3_1:66729551-66729570 Msa0369460:five_prime_UTR 25.0%
!! AATCTTTTGTGGGATTTTTT+TGG + chr3_1:66729659-66729678 Msa0369460:CDS 25.0%
!!! CAATTTCGGTATTTTTAATC+TGG + chr3_1:66729792-66729811 Msa0369460:intron 25.0%
!!! TATTTTTAATCTGGAAACAC+AGG + chr3_1:66729801-66729820 Msa0369460:intron 25.0%
!!! AATGCATTTTTAAGAAGGAT+GGG + chr3_1:66729825-66729844 Msa0369460:intron 25.0%
! ATCTTTGGTTAGAAAGTTTA+AGG + chr3_1:66729905-66729924 Msa0369460:CDS 25.0%
! TCTTTGGTTAGAAAGTTTAA+GGG + chr3_1:66729906-66729925 Msa0369460:CDS 25.0%
!!! TGAGATTTCATTTTTGATTC+TGG + chr3_1:66729972-66729991 Msa0369460:three_prime_UTR 25.0%
! AATGTGAAACACTAAATTCA+TGG - chr3_1:66730147-66730166 None:intergenic 25.0%
! CAAATGTCAACCAAAATTAT+TGG - chr3_1:66730281-66730300 None:intergenic 25.0%
!!! GACATTTGTTAGTTGATAAT+AGG + chr3_1:66730293-66730312 Msa0369460:three_prime_UTR 25.0%
TGTGATCATAACAAAAAGTG+TGG + chr3_1:66729197-66729216 Msa0369460:five_prime_UTR 30.0%
ACATTTGCGATCAGTTTATA+AGG - chr3_1:66729263-66729282 None:intergenic 30.0%
!! TAGGAGTATCATACATGTAT+TGG + chr3_1:66729491-66729510 Msa0369460:five_prime_UTR 30.0%
!! CTTCATTAACCCTATTTTTC+AGG + chr3_1:66729700-66729719 Msa0369460:CDS 30.0%
!! ACCGAAATTGAAGCATTTTT+GGG - chr3_1:66729782-66729801 None:intergenic 30.0%
!! TACCGAAATTGAAGCATTTT+TGG - chr3_1:66729783-66729802 None:intergenic 30.0%
!! CAATGCATTTTTAAGAAGGA+TGG + chr3_1:66729824-66729843 Msa0369460:intron 30.0%
CTTTGGTTAGAAAGTTTAAG+GGG + chr3_1:66729907-66729926 Msa0369460:CDS 30.0%
TGAAACACTAAATTCATGGA+AGG - chr3_1:66730143-66730162 None:intergenic 30.0%
TATCTCTCAGAAAGAAAACA+AGG + chr3_1:66730175-66730194 Msa0369460:three_prime_UTR 30.0%
ACAAGAAAGTCTAGTATGAT+AGG + chr3_1:66730227-66730246 Msa0369460:three_prime_UTR 30.0%
CAAGAAAGTCTAGTATGATA+GGG + chr3_1:66730228-66730247 Msa0369460:three_prime_UTR 30.0%
! GGGAAAAAAATGCATTTTAC+AGG + chr3_1:66730248-66730267 Msa0369460:three_prime_UTR 30.0%
! AGGAGCTAATCCAATAATTT+TGG + chr3_1:66730268-66730287 Msa0369460:three_prime_UTR 30.0%
AATCTTGAAAGTGGAGAAAA+TGG - chr3_1:66730384-66730403 None:intergenic 30.0%
TGTGATCATAACAAAAAGTG+TGG + chr3_1:66729197-66729216 Msa0369460:five_prime_UTR 30.0%
ACATTTGCGATCAGTTTATA+AGG - chr3_1:66729263-66729282 None:intergenic 30.0%
!! TAGGAGTATCATACATGTAT+TGG + chr3_1:66729491-66729510 Msa0369460:five_prime_UTR 30.0%
!! CTTCATTAACCCTATTTTTC+AGG + chr3_1:66729700-66729719 Msa0369460:CDS 30.0%
!! ACCGAAATTGAAGCATTTTT+GGG - chr3_1:66729782-66729801 None:intergenic 30.0%
!! TACCGAAATTGAAGCATTTT+TGG - chr3_1:66729783-66729802 None:intergenic 30.0%
!! CAATGCATTTTTAAGAAGGA+TGG + chr3_1:66729824-66729843 Msa0369460:intron 30.0%
CTTTGGTTAGAAAGTTTAAG+GGG + chr3_1:66729907-66729926 Msa0369460:CDS 30.0%
TGAAACACTAAATTCATGGA+AGG - chr3_1:66730143-66730162 None:intergenic 30.0%
TATCTCTCAGAAAGAAAACA+AGG + chr3_1:66730175-66730194 Msa0369460:three_prime_UTR 30.0%
ACAAGAAAGTCTAGTATGAT+AGG + chr3_1:66730227-66730246 Msa0369460:three_prime_UTR 30.0%
CAAGAAAGTCTAGTATGATA+GGG + chr3_1:66730228-66730247 Msa0369460:three_prime_UTR 30.0%
! GGGAAAAAAATGCATTTTAC+AGG + chr3_1:66730248-66730267 Msa0369460:three_prime_UTR 30.0%
! AGGAGCTAATCCAATAATTT+TGG + chr3_1:66730268-66730287 Msa0369460:three_prime_UTR 30.0%
AATCTTGAAAGTGGAGAAAA+TGG - chr3_1:66730384-66730403 None:intergenic 30.0%
TGCGATCAGTTTATAAGGAA+AGG - chr3_1:66729258-66729277 None:intergenic 35.0%
GTTCCGTTATTCATTCTATG+AGG - chr3_1:66729331-66729350 None:intergenic 35.0%
CTCATGTTGTGAGAAGAATT+GGG - chr3_1:66729360-66729379 None:intergenic 35.0%
!! AAATTTTGGAGGACACTTCT+TGG + chr3_1:66729459-66729478 Msa0369460:five_prime_UTR 35.0%
CACTTCTTGGATATGTATCT+AGG + chr3_1:66729472-66729491 Msa0369460:five_prime_UTR 35.0%
! TGTGCTAAAGCAATCTTTTG+TGG + chr3_1:66729648-66729667 Msa0369460:CDS 35.0%
! GTGCTAAAGCAATCTTTTGT+GGG + chr3_1:66729649-66729668 Msa0369460:CDS 35.0%
TAGGGATGACCTGAAAAATA+GGG - chr3_1:66729712-66729731 None:intergenic 35.0%
TTAGGGATGACCTGAAAAAT+AGG - chr3_1:66729713-66729732 None:intergenic 35.0%
!! CCGAAATTGAAGCATTTTTG+GGG - chr3_1:66729781-66729800 None:intergenic 35.0%
CCCCAAAAATGCTTCAATTT+CGG + chr3_1:66729778-66729797 Msa0369460:intron 35.0%
!! CAGGCAATGCATTTTTAAGA+AGG + chr3_1:66729820-66729839 Msa0369460:intron 35.0%
ATGAAATCTCAGAAGTTCAC+AGG - chr3_1:66729963-66729982 None:intergenic 35.0%
CGTAATAAACTCACCACATA+CGG - chr3_1:66729998-66730017 None:intergenic 35.0%
CGTATGTGGTGAGTTTATTA+CGG + chr3_1:66729996-66730015 Msa0369460:three_prime_UTR 35.0%
GTATGTGGTGAGTTTATTAC+GGG + chr3_1:66729997-66730016 Msa0369460:three_prime_UTR 35.0%
GGTGAATCAAATCTTGAAAG+TGG - chr3_1:66730393-66730412 None:intergenic 35.0%
TGCGATCAGTTTATAAGGAA+AGG - chr3_1:66729258-66729277 None:intergenic 35.0%
GTTCCGTTATTCATTCTATG+AGG - chr3_1:66729331-66729350 None:intergenic 35.0%
CTCATGTTGTGAGAAGAATT+GGG - chr3_1:66729360-66729379 None:intergenic 35.0%
!! AAATTTTGGAGGACACTTCT+TGG + chr3_1:66729459-66729478 Msa0369460:five_prime_UTR 35.0%
CACTTCTTGGATATGTATCT+AGG + chr3_1:66729472-66729491 Msa0369460:five_prime_UTR 35.0%
! TGTGCTAAAGCAATCTTTTG+TGG + chr3_1:66729648-66729667 Msa0369460:CDS 35.0%
! GTGCTAAAGCAATCTTTTGT+GGG + chr3_1:66729649-66729668 Msa0369460:CDS 35.0%
TAGGGATGACCTGAAAAATA+GGG - chr3_1:66729712-66729731 None:intergenic 35.0%
TTAGGGATGACCTGAAAAAT+AGG - chr3_1:66729713-66729732 None:intergenic 35.0%
!! CCGAAATTGAAGCATTTTTG+GGG - chr3_1:66729781-66729800 None:intergenic 35.0%
CCCCAAAAATGCTTCAATTT+CGG + chr3_1:66729778-66729797 Msa0369460:intron 35.0%
!! CAGGCAATGCATTTTTAAGA+AGG + chr3_1:66729820-66729839 Msa0369460:intron 35.0%
ATGAAATCTCAGAAGTTCAC+AGG - chr3_1:66729963-66729982 None:intergenic 35.0%
CGTAATAAACTCACCACATA+CGG - chr3_1:66729998-66730017 None:intergenic 35.0%
CGTATGTGGTGAGTTTATTA+CGG + chr3_1:66729996-66730015 Msa0369460:three_prime_UTR 35.0%
GTATGTGGTGAGTTTATTAC+GGG + chr3_1:66729997-66730016 Msa0369460:three_prime_UTR 35.0%
GGTGAATCAAATCTTGAAAG+TGG - chr3_1:66730393-66730412 None:intergenic 35.0%
CCTCATGTTGTGAGAAGAAT+TGG - chr3_1:66729361-66729380 None:intergenic 40.0%
CCAATTCTTCTCACAACATG+AGG + chr3_1:66729358-66729377 Msa0369460:five_prime_UTR 40.0%
! CAGAACAAGGCTCATGTTTT+TGG + chr3_1:66729381-66729400 Msa0369460:five_prime_UTR 40.0%
CGATTGTGAAGGTTGAGAAT+GGG - chr3_1:66729619-66729638 None:intergenic 40.0%
CACTCAAAAAGCGATTGTGA+AGG - chr3_1:66729630-66729649 None:intergenic 40.0%
!!! GTGGGATTTTTTTGGCAAGT+AGG + chr3_1:66729667-66729686 Msa0369460:CDS 40.0%
!!! TTTTCAGGTCATCCCTAACT+TGG + chr3_1:66729715-66729734 Msa0369460:CDS 40.0%
AAGAATACCTGTGTTCTCCT+AGG - chr3_1:66729758-66729777 None:intergenic 40.0%
AAAGACACGGCATAGTACAA+AGG - chr3_1:66729870-66729889 None:intergenic 40.0%
TTCTAACCAAAGATGAGCAC+AGG - chr3_1:66729899-66729918 None:intergenic 40.0%
TCAGAAGTTCACAGGAGAAT+AGG - chr3_1:66729955-66729974 None:intergenic 40.0%
!! TTTTTGATTCTGGCCGTATG+TGG + chr3_1:66729982-66730001 Msa0369460:three_prime_UTR 40.0%
CTGCAGAATGTTCCATATCA+AGG - chr3_1:66730096-66730115 None:intergenic 40.0%
CTCTCAGAAAGAAAACAAGG+AGG + chr3_1:66730178-66730197 Msa0369460:three_prime_UTR 40.0%
GAAAGAAAACAAGGAGGATG+AGG + chr3_1:66730184-66730203 Msa0369460:three_prime_UTR 40.0%
TATCTCCGTGTTTGTGTTGT+AGG - chr3_1:66729083-66729102 None:intergenic 40.0%
CCTCATGTTGTGAGAAGAAT+TGG - chr3_1:66729361-66729380 None:intergenic 40.0%
CCAATTCTTCTCACAACATG+AGG + chr3_1:66729358-66729377 Msa0369460:five_prime_UTR 40.0%
! CAGAACAAGGCTCATGTTTT+TGG + chr3_1:66729381-66729400 Msa0369460:five_prime_UTR 40.0%
CGATTGTGAAGGTTGAGAAT+GGG - chr3_1:66729619-66729638 None:intergenic 40.0%
CACTCAAAAAGCGATTGTGA+AGG - chr3_1:66729630-66729649 None:intergenic 40.0%
!!! GTGGGATTTTTTTGGCAAGT+AGG + chr3_1:66729667-66729686 Msa0369460:CDS 40.0%
!!! TTTTCAGGTCATCCCTAACT+TGG + chr3_1:66729715-66729734 Msa0369460:CDS 40.0%
AAGAATACCTGTGTTCTCCT+AGG - chr3_1:66729758-66729777 None:intergenic 40.0%
AAAGACACGGCATAGTACAA+AGG - chr3_1:66729870-66729889 None:intergenic 40.0%
TTCTAACCAAAGATGAGCAC+AGG - chr3_1:66729899-66729918 None:intergenic 40.0%
TCAGAAGTTCACAGGAGAAT+AGG - chr3_1:66729955-66729974 None:intergenic 40.0%
!! TTTTTGATTCTGGCCGTATG+TGG + chr3_1:66729982-66730001 Msa0369460:three_prime_UTR 40.0%
CTGCAGAATGTTCCATATCA+AGG - chr3_1:66730096-66730115 None:intergenic 40.0%
CTCTCAGAAAGAAAACAAGG+AGG + chr3_1:66730178-66730197 Msa0369460:three_prime_UTR 40.0%
GAAAGAAAACAAGGAGGATG+AGG + chr3_1:66730184-66730203 Msa0369460:three_prime_UTR 40.0%
TCACAACATGAGGCAGAACA+AGG + chr3_1:66729368-66729387 Msa0369460:five_prime_UTR 45.0%
GCGATTGTGAAGGTTGAGAA+TGG - chr3_1:66729620-66729639 None:intergenic 45.0%
TCATCCCTAACTTGGACTTG+TGG + chr3_1:66729723-66729742 Msa0369460:CDS 45.0%
TGGTCCACAAGTCCAAGTTA+GGG - chr3_1:66729730-66729749 None:intergenic 45.0%
AAGTTTAAGGGGCCATCTGA+AGG + chr3_1:66729918-66729937 Msa0369460:CDS 45.0%
CTGCATTTCAGATACCCTAG+TGG + chr3_1:66730055-66730074 Msa0369460:three_prime_UTR 45.0%
GCTCTGATGCTTCCTTGATA+TGG + chr3_1:66730081-66730100 Msa0369460:three_prime_UTR 45.0%
TGATTTAGCTGGGGGAGAAA+AGG - chr3_1:66730414-66730433 None:intergenic 45.0%
TCACAACATGAGGCAGAACA+AGG + chr3_1:66729368-66729387 Msa0369460:five_prime_UTR 45.0%
GCGATTGTGAAGGTTGAGAA+TGG - chr3_1:66729620-66729639 None:intergenic 45.0%
TCATCCCTAACTTGGACTTG+TGG + chr3_1:66729723-66729742 Msa0369460:CDS 45.0%
TGGTCCACAAGTCCAAGTTA+GGG - chr3_1:66729730-66729749 None:intergenic 45.0%
AAGTTTAAGGGGCCATCTGA+AGG + chr3_1:66729918-66729937 Msa0369460:CDS 45.0%
CTGCATTTCAGATACCCTAG+TGG + chr3_1:66730055-66730074 Msa0369460:three_prime_UTR 45.0%
GCTCTGATGCTTCCTTGATA+TGG + chr3_1:66730081-66730100 Msa0369460:three_prime_UTR 45.0%
TGATTTAGCTGGGGGAGAAA+AGG - chr3_1:66730414-66730433 None:intergenic 45.0%
CTGGTCCACAAGTCCAAGTT+AGG - chr3_1:66729731-66729750 None:intergenic 50.0%
TCACAGGAGAATAGGTGCTC+CGG - chr3_1:66729947-66729966 None:intergenic 50.0%
AGCATCAGAGCTGACCACTA+GGG - chr3_1:66730072-66730091 None:intergenic 50.0%
AAGCATCAGAGCTGACCACT+AGG - chr3_1:66730073-66730092 None:intergenic 50.0%
CTGGTCCACAAGTCCAAGTT+AGG - chr3_1:66729731-66729750 None:intergenic 50.0%
TCACAGGAGAATAGGTGCTC+CGG - chr3_1:66729947-66729966 None:intergenic 50.0%
AGCATCAGAGCTGACCACTA+GGG - chr3_1:66730072-66730091 None:intergenic 50.0%
AAGCATCAGAGCTGACCACT+AGG - chr3_1:66730073-66730092 None:intergenic 50.0%
ACTTGTGGACCAGCTTGCCT+AGG + chr3_1:66729738-66729757 Msa0369460:CDS 55.0%
CTGTGTTCTCCTAGGCAAGC+TGG - chr3_1:66729750-66729769 None:intergenic 55.0%
CAGCTTGCCTAGGAGAACAC+AGG + chr3_1:66729748-66729767 Msa0369460:CDS 55.0%
GCACAGGCTCTGCAAAGACA+CGG - chr3_1:66729883-66729902 None:intergenic 55.0%
GCAGAGCCTGTGCTCATCTT+TGG + chr3_1:66729890-66729909 Msa0369460:intron 55.0%
ACTTGTGGACCAGCTTGCCT+AGG + chr3_1:66729738-66729757 Msa0369460:CDS 55.0%
CTGTGTTCTCCTAGGCAAGC+TGG - chr3_1:66729750-66729769 None:intergenic 55.0%
CAGCTTGCCTAGGAGAACAC+AGG + chr3_1:66729748-66729767 Msa0369460:CDS 55.0%
GCACAGGCTCTGCAAAGACA+CGG - chr3_1:66729883-66729902 None:intergenic 55.0%
GCAGAGCCTGTGCTCATCTT+TGG + chr3_1:66729890-66729909 Msa0369460:intron 55.0%
AGGGGCCATCTGAAGGACAC+CGG + chr3_1:66729925-66729944 Msa0369460:CDS 60.0%
GTGCTCCGGTGTCCTTCAGA+TGG - chr3_1:66729933-66729952 None:intergenic 60.0%
AGGGGCCATCTGAAGGACAC+CGG + chr3_1:66729925-66729944 Msa0369460:CDS 60.0%
GTGCTCCGGTGTCCTTCAGA+TGG - chr3_1:66729933-66729952 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 66729082 66730440 66729082 ID=Msa0369460;Name=Msa0369460
chr3_1 mRNA 66729082 66730440 66729082 ID=Msa0369460-mRNA-1;Parent=Msa0369460;Name=Msa0369460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=527|1|1|1|1|1|2|474|77
chr3_1 exon 66729082 66729769 66729082 ID=Msa0369460-mRNA-1:exon:13596;Parent=Msa0369460-mRNA-1
chr3_1 exon 66729894 66730440 66729894 ID=Msa0369460-mRNA-1:exon:13597;Parent=Msa0369460-mRNA-1
chr3_1 five_prime_UTR 66729082 66729608 66729082 ID=Msa0369460-mRNA-1:five_prime_utr;Parent=Msa0369460-mRNA-1
chr3_1 CDS 66729609 66729769 66729609 ID=Msa0369460-mRNA-1:cds;Parent=Msa0369460-mRNA-1
chr3_1 CDS 66729894 66729966 66729894 ID=Msa0369460-mRNA-1:cds;Parent=Msa0369460-mRNA-1
chr3_1 three_prime_UTR 66729967 66730440 66729967 ID=Msa0369460-mRNA-1:three_prime_utr;Parent=Msa0369460-mRNA-1
Gene Sequence

>Msa0369460

ATGTTATCCCATTCTCAACCTTCACAATCGCTTTTTGAGTGTGCTAAAGCAATCTTTTGTGGGATTTTTTTGGCAAGTAGGACTGAGATTTCTTCATTAACCCTATTTTTCAGGTCATCCCTAACTTGGACTTGTGGACCAGCTTGCCTAGGAGAACACAGAGCCTGTGCTCATCTTTGGTTAGAAAGTTTAAGGGGCCATCTGAAGGACACCGGAGCACCTATTCTCCTGTGA
Protein sequence

>Msa0369460

MLSHSQPSQSLFECAKAIFCGIFLASRTEISSLTLFFRSSLTWTCGPACLGEHRACAHLWLESLRGHLKDTGAPILL*