AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0840210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840210 MtrunA17_Chr7g0249561 74.242 132 32 1 1 132 1 130 1.52e-66 197
Msa0840210 MtrunA17_Chr7g0249801 39.695 131 66 3 1 131 1 118 1.76e-26 95.9
Msa0840210 MtrunA17_Chr4g0076301 36.290 124 66 2 18 132 4 123 1.27e-17 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840210 AT1G65290.1 62.121 132 44 3 1 132 1 126 6.61e-49 153
Msa0840210 AT2G44620.1 43.939 132 62 5 1 131 1 121 1.11e-25 94.4
Msa0840210 AT5G47630.1 38.750 80 49 0 53 132 48 127 2.29e-13 62.8
Msa0840210 AT5G47630.2 38.750 80 49 0 53 132 48 127 2.29e-13 62.8
Msa0840210 AT5G47630.3 38.750 80 49 0 53 132 48 127 2.29e-13 62.8
Msa0840210 AT3G05020.1 35.955 89 57 0 37 125 41 129 3.53e-11 57.4

Find 54 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGCTCGGGGAAGCGGTTT+GGG 0.283525 5_4:+15808299 Msa0840210:CDS
GGCGGCTCGGGGAAGCGGTT+TGG 0.290380 5_4:+15808298 Msa0840210:CDS
TGACAATGTTGATCCTTCTA+AGG 0.294761 5_4:+15808496 Msa0840210:CDS
ATGGATCTTGATACAGAGTT+TGG 0.311699 5_4:+15809360 Msa0840210:CDS
AAGCATGAACCTCAACTCTT+AGG 0.355069 5_4:-15808334 None:intergenic
ATCAACAACAATGGCGGCTC+GGG 0.358776 5_4:+15808286 Msa0840210:exon
AACACACTTCAGGGATTTAG+AGG 0.382604 5_4:-15809603 None:intergenic
TCCACAGCGAAACACACTTC+AGG 0.404996 5_4:-15809613 None:intergenic
GAAATCAACAGCTTGATTAA+CGG 0.409809 5_4:-15809421 None:intergenic
ATGGAAGTGAAAACCTTAGA+AGG 0.418506 5_4:-15808509 None:intergenic
AAATTTACAACTAGCCTCAT+AGG 0.436317 5_4:-15809479 None:intergenic
TTTCTATGATGACCTTGGAT+TGG 0.444522 5_4:+15809311 Msa0840210:CDS
TCAGGGATTTAGAGGGGCGG+GGG 0.453801 5_4:-15809595 None:intergenic
AAGATCCATACAGATCTCTA+TGG 0.455448 5_4:-15809346 None:intergenic
CGCAGCTTTATGATAAAGAA+AGG 0.457123 5_4:-15809504 None:intergenic
GTCGCTTCAGCGAAGAGATC+AGG 0.457126 5_4:+15808414 Msa0840210:CDS
TTCAGGGATTTAGAGGGGCG+GGG 0.459574 5_4:-15809596 None:intergenic
ACAATGGCGGCTCGGGGAAG+CGG 0.466910 5_4:+15808293 Msa0840210:exon
GAGGAGTACGCAAAGGAGAT+TGG 0.466971 5_4:-15808370 None:intergenic
CAGCGACGAAGGAAGGAATT+CGG 0.467889 5_4:-15808392 None:intergenic
CAGGGATTTAGAGGGGCGGG+GGG 0.467914 5_4:-15809594 None:intergenic
CATCATAGAAATGAGCAGTT+GGG 0.468087 5_4:-15809299 None:intergenic
TGCTTATTTCATGTTATGCA+AGG 0.469956 5_4:+15809816 Msa0840210:three_prime_UTR
TTTCATTGCATCTCATCCTC+AGG 0.474149 5_4:+15809440 Msa0840210:CDS
GCTCATTTCTATGATGACCT+TGG 0.491539 5_4:+15809306 Msa0840210:CDS
CTTCAGGGATTTAGAGGGGC+GGG 0.493115 5_4:-15809597 None:intergenic
CCACAGCGAAACACACTTCA+GGG 0.495620 5_4:-15809612 None:intergenic
TATCAACAACAATGGCGGCT+CGG 0.524282 5_4:+15808285 Msa0840210:exon
ACACAGTGAACGAATAATGA+AGG 0.537121 5_4:-15808239 None:intergenic
TCATCATAGAAATGAGCAGT+TGG 0.537450 5_4:-15809300 None:intergenic
GAAGCGACAGCGACGAAGGA+AGG 0.545017 5_4:-15808399 None:intergenic
CAAAAGTAAAATACCACTCA+AGG 0.550074 5_4:-15809643 None:intergenic
CGCTGAAGCGACAGCGACGA+AGG 0.552195 5_4:-15808403 None:intergenic
GCTTATTTCATGTTATGCAA+GGG 0.561293 5_4:+15809817 Msa0840210:three_prime_UTR
AGACGCCATAGAGATCTGTA+TGG 0.564613 5_4:+15809341 Msa0840210:CDS
ACACACTTCAGGGATTTAGA+GGG 0.576013 5_4:-15809602 None:intergenic
TGTGGATGTGCGACCTTGAG+TGG 0.583478 5_4:+15809630 Msa0840210:three_prime_UTR
ATCATAGAAATGAGCAGTTG+GGG 0.586456 5_4:-15809298 None:intergenic
GATGAATTACCTAAGAGTTG+AGG 0.587013 5_4:+15808325 Msa0840210:CDS
ACTTCAGGGATTTAGAGGGG+CGG 0.587335 5_4:-15809598 None:intergenic
AGTTTAGAAGCAAACAAGCA+AGG 0.592842 5_4:-15809559 None:intergenic
TTTATGATTATCGAATCTTG+AGG 0.598752 5_4:+15809735 Msa0840210:three_prime_UTR
ACTGTGTTGCTGTGAGTGTG+AGG 0.598812 5_4:+15808255 Msa0840210:five_prime_UTR
CGTCTAAACTATCCAATCCA+AGG 0.606873 5_4:-15809323 None:intergenic
GAGTACGCAAAGGAGATTGG+TGG 0.606939 5_4:-15808367 None:intergenic
TTTACAACTAGCCTCATAGG+AGG 0.608898 5_4:-15809476 None:intergenic
AAGTAGTCTAACCTCCTATG+AGG 0.615125 5_4:+15809465 Msa0840210:exon
CACAGTGAACGAATAATGAA+GGG 0.617966 5_4:-15808238 None:intergenic
TCAACAACAATGGCGGCTCG+GGG 0.630083 5_4:+15808287 Msa0840210:exon
CCCTGAAGTGTGTTTCGCTG+TGG 0.644881 5_4:+15809612 Msa0840210:three_prime_UTR
CACACTTCAGGGATTTAGAG+GGG 0.657579 5_4:-15809601 None:intergenic
GAATTCGGAGGAGTACGCAA+AGG 0.668596 5_4:-15808377 None:intergenic
AGGTTAGACTACTTCGCCTG+AGG 0.672291 5_4:-15809456 None:intergenic
CGACGAAGGAAGGAATTCGG+AGG 0.686278 5_4:-15808389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAATAAATGAAGAAATT+AGG - chr5_4:15808541-15808560 None:intergenic 15.0%
!!! CTATTTTCAATCTTTAATTT+AGG + chr5_4:15808577-15808596 Msa0840210:intron 15.0%
!!! TATTTTACTTTTGAAGATAT+TGG + chr5_4:15809653-15809672 Msa0840210:three_prime_UTR 15.0%
!! TGAAAATAAATGAAGAAATT+AGG - chr5_4:15808541-15808560 None:intergenic 15.0%
!!! CTATTTTCAATCTTTAATTT+AGG + chr5_4:15808577-15808596 Msa0840210:intron 15.0%
!!! TATTTTACTTTTGAAGATAT+TGG + chr5_4:15809653-15809672 Msa0840210:three_prime_UTR 15.0%
!!! GTTTTTTTTATCAACAACAA+TGG + chr5_4:15808277-15808296 Msa0840210:exon 20.0%
!! TAAATGAAGAAATTAGGTTA+AGG - chr5_4:15808535-15808554 None:intergenic 20.0%
!! AAGCCAAAGATATAATATAA+CGG - chr5_4:15809225-15809244 None:intergenic 20.0%
!!! ATTTTTACTGACATGAAAAA+TGG - chr5_4:15809706-15809725 None:intergenic 20.0%
!!! GTTTTTTTTATCAACAACAA+TGG + chr5_4:15808277-15808296 Msa0840210:exon 20.0%
!! TAAATGAAGAAATTAGGTTA+AGG - chr5_4:15808535-15808554 None:intergenic 20.0%
!! AAGCCAAAGATATAATATAA+CGG - chr5_4:15809225-15809244 None:intergenic 20.0%
!!! ATTTTTACTGACATGAAAAA+TGG - chr5_4:15809706-15809725 None:intergenic 20.0%
!!! TTTTTTATCAACAACAATGG+CGG + chr5_4:15808280-15808299 Msa0840210:exon 25.0%
!! TTATCCTTTTATGTTGTGTT+TGG - chr5_4:15808758-15808777 None:intergenic 25.0%
!! ATAAGTTGTCTTTTCAAACA+TGG - chr5_4:15808918-15808937 None:intergenic 25.0%
! GTACCGTTATATTATATCTT+TGG + chr5_4:15809219-15809238 Msa0840210:intron 25.0%
! CAAAAAATGTCTTAAAGTTG+TGG - chr5_4:15809539-15809558 None:intergenic 25.0%
! TTTATGATTATCGAATCTTG+AGG + chr5_4:15809735-15809754 Msa0840210:three_prime_UTR 25.0%
!!! TTTTTTATCAACAACAATGG+CGG + chr5_4:15808280-15808299 Msa0840210:exon 25.0%
!! TTATCCTTTTATGTTGTGTT+TGG - chr5_4:15808758-15808777 None:intergenic 25.0%
!! ATAAGTTGTCTTTTCAAACA+TGG - chr5_4:15808918-15808937 None:intergenic 25.0%
! GTACCGTTATATTATATCTT+TGG + chr5_4:15809219-15809238 Msa0840210:intron 25.0%
! CAAAAAATGTCTTAAAGTTG+TGG - chr5_4:15809539-15809558 None:intergenic 25.0%
! TTTATGATTATCGAATCTTG+AGG + chr5_4:15809735-15809754 Msa0840210:three_prime_UTR 25.0%
!!! ATTGTCAAATTTTTTGACGC+AGG - chr5_4:15808483-15808502 None:intergenic 30.0%
TGAAGAAATTAGGTTAAGGA+TGG - chr5_4:15808531-15808550 None:intergenic 30.0%
! TGACCAATTCTTGTAAGTTT+TGG + chr5_4:15808622-15808641 Msa0840210:intron 30.0%
TGATCCAAACACAACATAAA+AGG + chr5_4:15808751-15808770 Msa0840210:intron 30.0%
! GCTAAGTGAAGACTATATTT+TGG + chr5_4:15808882-15808901 Msa0840210:intron 30.0%
!! AAGTGAAGACTATATTTTGG+AGG + chr5_4:15808885-15808904 Msa0840210:intron 30.0%
GTTTATCTGAGCTTATTTAC+TGG - chr5_4:15809108-15809127 None:intergenic 30.0%
TAAAGTAAAAGTCTACACTG+AGG - chr5_4:15809144-15809163 None:intergenic 30.0%
! ATCTTGTCTACTTCATTTTC+AGG - chr5_4:15809396-15809415 None:intergenic 30.0%
GAAATCAACAGCTTGATTAA+CGG - chr5_4:15809424-15809443 None:intergenic 30.0%
! AAATTTACAACTAGCCTCAT+AGG - chr5_4:15809482-15809501 None:intergenic 30.0%
CAAAAGTAAAATACCACTCA+AGG - chr5_4:15809646-15809665 None:intergenic 30.0%
TGCTTATTTCATGTTATGCA+AGG + chr5_4:15809816-15809835 Msa0840210:three_prime_UTR 30.0%
GCTTATTTCATGTTATGCAA+GGG + chr5_4:15809817-15809836 Msa0840210:three_prime_UTR 30.0%
!!! ATTGTCAAATTTTTTGACGC+AGG - chr5_4:15808483-15808502 None:intergenic 30.0%
TGAAGAAATTAGGTTAAGGA+TGG - chr5_4:15808531-15808550 None:intergenic 30.0%
! TGACCAATTCTTGTAAGTTT+TGG + chr5_4:15808622-15808641 Msa0840210:intron 30.0%
TGATCCAAACACAACATAAA+AGG + chr5_4:15808751-15808770 Msa0840210:intron 30.0%
! GCTAAGTGAAGACTATATTT+TGG + chr5_4:15808882-15808901 Msa0840210:intron 30.0%
!! AAGTGAAGACTATATTTTGG+AGG + chr5_4:15808885-15808904 Msa0840210:intron 30.0%
GTTTATCTGAGCTTATTTAC+TGG - chr5_4:15809108-15809127 None:intergenic 30.0%
TAAAGTAAAAGTCTACACTG+AGG - chr5_4:15809144-15809163 None:intergenic 30.0%
! ATCTTGTCTACTTCATTTTC+AGG - chr5_4:15809396-15809415 None:intergenic 30.0%
GAAATCAACAGCTTGATTAA+CGG - chr5_4:15809424-15809443 None:intergenic 30.0%
! AAATTTACAACTAGCCTCAT+AGG - chr5_4:15809482-15809501 None:intergenic 30.0%
CAAAAGTAAAATACCACTCA+AGG - chr5_4:15809646-15809665 None:intergenic 30.0%
TGCTTATTTCATGTTATGCA+AGG + chr5_4:15809816-15809835 Msa0840210:three_prime_UTR 30.0%
GCTTATTTCATGTTATGCAA+GGG + chr5_4:15809817-15809836 Msa0840210:three_prime_UTR 30.0%
CACAGTGAACGAATAATGAA+GGG - chr5_4:15808241-15808260 None:intergenic 35.0%
ACACAGTGAACGAATAATGA+AGG - chr5_4:15808242-15808261 None:intergenic 35.0%
GATGAATTACCTAAGAGTTG+AGG + chr5_4:15808325-15808344 Msa0840210:CDS 35.0%
! GAGAACTTTTCTCGACAAAT+CGG + chr5_4:15808436-15808455 Msa0840210:CDS 35.0%
TGACAATGTTGATCCTTCTA+AGG + chr5_4:15808496-15808515 Msa0840210:CDS 35.0%
ATGGAAGTGAAAACCTTAGA+AGG - chr5_4:15808512-15808531 None:intergenic 35.0%
AGGCCAAAACTTACAAGAAT+TGG - chr5_4:15808628-15808647 None:intergenic 35.0%
! TTGTGTTTGGATCAGTGTTT+TGG - chr5_4:15808745-15808764 None:intergenic 35.0%
! AAGTTCTAGTCCTCGAAATA+GGG + chr5_4:15808804-15808823 Msa0840210:intron 35.0%
GTACAATAAACCCAAAAGTG+CGG + chr5_4:15808836-15808855 Msa0840210:intron 35.0%
! CTGTTTTCATAAGCTATCCT+AGG + chr5_4:15808990-15809009 Msa0840210:intron 35.0%
TTATTTCCGTAAGCTATCCT+AGG - chr5_4:15809010-15809029 None:intergenic 35.0%
TTAATGTGCTTGTGTCAGTT+TGG + chr5_4:15809167-15809186 Msa0840210:intron 35.0%
ATATAACGGTACACAAATGC+TGG - chr5_4:15809211-15809230 None:intergenic 35.0%
ATCATAGAAATGAGCAGTTG+GGG - chr5_4:15809301-15809320 None:intergenic 35.0%
CATCATAGAAATGAGCAGTT+GGG - chr5_4:15809302-15809321 None:intergenic 35.0%
TCATCATAGAAATGAGCAGT+TGG - chr5_4:15809303-15809322 None:intergenic 35.0%
TTTCTATGATGACCTTGGAT+TGG + chr5_4:15809311-15809330 Msa0840210:CDS 35.0%
AAGATCCATACAGATCTCTA+TGG - chr5_4:15809349-15809368 None:intergenic 35.0%
ATGGATCTTGATACAGAGTT+TGG + chr5_4:15809360-15809379 Msa0840210:CDS 35.0%
! CGCAGCTTTATGATAAAGAA+AGG - chr5_4:15809507-15809526 None:intergenic 35.0%
AGTTTAGAAGCAAACAAGCA+AGG - chr5_4:15809562-15809581 None:intergenic 35.0%
CACAGTGAACGAATAATGAA+GGG - chr5_4:15808241-15808260 None:intergenic 35.0%
ACACAGTGAACGAATAATGA+AGG - chr5_4:15808242-15808261 None:intergenic 35.0%
GATGAATTACCTAAGAGTTG+AGG + chr5_4:15808325-15808344 Msa0840210:CDS 35.0%
! GAGAACTTTTCTCGACAAAT+CGG + chr5_4:15808436-15808455 Msa0840210:CDS 35.0%
TGACAATGTTGATCCTTCTA+AGG + chr5_4:15808496-15808515 Msa0840210:CDS 35.0%
ATGGAAGTGAAAACCTTAGA+AGG - chr5_4:15808512-15808531 None:intergenic 35.0%
AGGCCAAAACTTACAAGAAT+TGG - chr5_4:15808628-15808647 None:intergenic 35.0%
! TTGTGTTTGGATCAGTGTTT+TGG - chr5_4:15808745-15808764 None:intergenic 35.0%
! AAGTTCTAGTCCTCGAAATA+GGG + chr5_4:15808804-15808823 Msa0840210:intron 35.0%
GTACAATAAACCCAAAAGTG+CGG + chr5_4:15808836-15808855 Msa0840210:intron 35.0%
! CTGTTTTCATAAGCTATCCT+AGG + chr5_4:15808990-15809009 Msa0840210:intron 35.0%
TTATTTCCGTAAGCTATCCT+AGG - chr5_4:15809010-15809029 None:intergenic 35.0%
TTAATGTGCTTGTGTCAGTT+TGG + chr5_4:15809167-15809186 Msa0840210:intron 35.0%
ATATAACGGTACACAAATGC+TGG - chr5_4:15809211-15809230 None:intergenic 35.0%
ATCATAGAAATGAGCAGTTG+GGG - chr5_4:15809301-15809320 None:intergenic 35.0%
CATCATAGAAATGAGCAGTT+GGG - chr5_4:15809302-15809321 None:intergenic 35.0%
TCATCATAGAAATGAGCAGT+TGG - chr5_4:15809303-15809322 None:intergenic 35.0%
TTTCTATGATGACCTTGGAT+TGG + chr5_4:15809311-15809330 Msa0840210:CDS 35.0%
AAGATCCATACAGATCTCTA+TGG - chr5_4:15809349-15809368 None:intergenic 35.0%
ATGGATCTTGATACAGAGTT+TGG + chr5_4:15809360-15809379 Msa0840210:CDS 35.0%
! CGCAGCTTTATGATAAAGAA+AGG - chr5_4:15809507-15809526 None:intergenic 35.0%
AGTTTAGAAGCAAACAAGCA+AGG - chr5_4:15809562-15809581 None:intergenic 35.0%
AAGCATGAACCTCAACTCTT+AGG - chr5_4:15808337-15808356 None:intergenic 40.0%
! AACTTTTCTCGACAAATCGG+AGG + chr5_4:15808439-15808458 Msa0840210:CDS 40.0%
! CAAGTTCTAGTCCTCGAAAT+AGG + chr5_4:15808803-15808822 Msa0840210:intron 40.0%
! CATAGTCTTACCCTATTTCG+AGG - chr5_4:15808817-15808836 None:intergenic 40.0%
AGCTATCCTAGGATAGCTTA+CGG + chr5_4:15809001-15809020 Msa0840210:intron 40.0%
GGAAACAGCTTATGACATGT+AGG - chr5_4:15809046-15809065 None:intergenic 40.0%
! TGACAAAAGCAAAGAAGCAC+AGG - chr5_4:15809273-15809292 None:intergenic 40.0%
!! TGTGCTTCTTTGCTTTTGTC+AGG + chr5_4:15809272-15809291 Msa0840210:intron 40.0%
GCTCATTTCTATGATGACCT+TGG + chr5_4:15809306-15809325 Msa0840210:CDS 40.0%
CGTCTAAACTATCCAATCCA+AGG - chr5_4:15809326-15809345 None:intergenic 40.0%
TTTCATTGCATCTCATCCTC+AGG + chr5_4:15809440-15809459 Msa0840210:CDS 40.0%
AAGTAGTCTAACCTCCTATG+AGG + chr5_4:15809465-15809484 Msa0840210:exon 40.0%
! TTTACAACTAGCCTCATAGG+AGG - chr5_4:15809479-15809498 None:intergenic 40.0%
ACACACTTCAGGGATTTAGA+GGG - chr5_4:15809605-15809624 None:intergenic 40.0%
AACACACTTCAGGGATTTAG+AGG - chr5_4:15809606-15809625 None:intergenic 40.0%
AAGCATGAACCTCAACTCTT+AGG - chr5_4:15808337-15808356 None:intergenic 40.0%
! AACTTTTCTCGACAAATCGG+AGG + chr5_4:15808439-15808458 Msa0840210:CDS 40.0%
! CAAGTTCTAGTCCTCGAAAT+AGG + chr5_4:15808803-15808822 Msa0840210:intron 40.0%
! CATAGTCTTACCCTATTTCG+AGG - chr5_4:15808817-15808836 None:intergenic 40.0%
AGCTATCCTAGGATAGCTTA+CGG + chr5_4:15809001-15809020 Msa0840210:intron 40.0%
GGAAACAGCTTATGACATGT+AGG - chr5_4:15809046-15809065 None:intergenic 40.0%
! TGACAAAAGCAAAGAAGCAC+AGG - chr5_4:15809273-15809292 None:intergenic 40.0%
!! TGTGCTTCTTTGCTTTTGTC+AGG + chr5_4:15809272-15809291 Msa0840210:intron 40.0%
GCTCATTTCTATGATGACCT+TGG + chr5_4:15809306-15809325 Msa0840210:CDS 40.0%
CGTCTAAACTATCCAATCCA+AGG - chr5_4:15809326-15809345 None:intergenic 40.0%
TTTCATTGCATCTCATCCTC+AGG + chr5_4:15809440-15809459 Msa0840210:CDS 40.0%
AAGTAGTCTAACCTCCTATG+AGG + chr5_4:15809465-15809484 Msa0840210:exon 40.0%
! TTTACAACTAGCCTCATAGG+AGG - chr5_4:15809479-15809498 None:intergenic 40.0%
ACACACTTCAGGGATTTAGA+GGG - chr5_4:15809605-15809624 None:intergenic 40.0%
AACACACTTCAGGGATTTAG+AGG - chr5_4:15809606-15809625 None:intergenic 40.0%
TATCAACAACAATGGCGGCT+CGG + chr5_4:15808285-15808304 Msa0840210:exon 45.0%
!! TTGGCCTCTGTTTGTTTCTG+CGG + chr5_4:15808641-15808660 Msa0840210:intron 45.0%
TTTGCCGCAGAAACAAACAG+AGG - chr5_4:15808648-15808667 None:intergenic 45.0%
ATCAGCTCAAACGCACGTTT+GGG - chr5_4:15808676-15808695 None:intergenic 45.0%
AAGTTGTTGTCACCTGACTG+AGG + chr5_4:15808777-15808796 Msa0840210:intron 45.0%
TAGAACTTGTGACCTCAGTC+AGG - chr5_4:15808792-15808811 None:intergenic 45.0%
AGACTGTGTGATAGAGCTTG+TGG - chr5_4:15809067-15809086 None:intergenic 45.0%
AGACGCCATAGAGATCTGTA+TGG + chr5_4:15809341-15809360 Msa0840210:CDS 45.0%
CACACTTCAGGGATTTAGAG+GGG - chr5_4:15809604-15809623 None:intergenic 45.0%
TATCAACAACAATGGCGGCT+CGG + chr5_4:15808285-15808304 Msa0840210:exon 45.0%
!! TTGGCCTCTGTTTGTTTCTG+CGG + chr5_4:15808641-15808660 Msa0840210:intron 45.0%
TTTGCCGCAGAAACAAACAG+AGG - chr5_4:15808648-15808667 None:intergenic 45.0%
ATCAGCTCAAACGCACGTTT+GGG - chr5_4:15808676-15808695 None:intergenic 45.0%
AAGTTGTTGTCACCTGACTG+AGG + chr5_4:15808777-15808796 Msa0840210:intron 45.0%
TAGAACTTGTGACCTCAGTC+AGG - chr5_4:15808792-15808811 None:intergenic 45.0%
AGACTGTGTGATAGAGCTTG+TGG - chr5_4:15809067-15809086 None:intergenic 45.0%
AGACGCCATAGAGATCTGTA+TGG + chr5_4:15809341-15809360 Msa0840210:CDS 45.0%
CACACTTCAGGGATTTAGAG+GGG - chr5_4:15809604-15809623 None:intergenic 45.0%
! ACTGTGTTGCTGTGAGTGTG+AGG + chr5_4:15808255-15808274 Msa0840210:five_prime_UTR 50.0%
ATCAACAACAATGGCGGCTC+GGG + chr5_4:15808286-15808305 Msa0840210:exon 50.0%
GAGTACGCAAAGGAGATTGG+TGG - chr5_4:15808370-15808389 None:intergenic 50.0%
! GAGGAGTACGCAAAGGAGAT+TGG - chr5_4:15808373-15808392 None:intergenic 50.0%
! GAATTCGGAGGAGTACGCAA+AGG - chr5_4:15808380-15808399 None:intergenic 50.0%
CAGCGACGAAGGAAGGAATT+CGG - chr5_4:15808395-15808414 None:intergenic 50.0%
CATCAGCTCAAACGCACGTT+TGG - chr5_4:15808677-15808696 None:intergenic 50.0%
! ATAAACCCAAAAGTGCGGCG+CGG + chr5_4:15808841-15808860 Msa0840210:intron 50.0%
GTCTACACTGAGGCTCTGTT+TGG - chr5_4:15809134-15809153 None:intergenic 50.0%
GGTACACAAATGCTGGCAGA+GGG - chr5_4:15809204-15809223 None:intergenic 50.0%
AGGTTAGACTACTTCGCCTG+AGG - chr5_4:15809459-15809478 None:intergenic 50.0%
ACTTCAGGGATTTAGAGGGG+CGG - chr5_4:15809601-15809620 None:intergenic 50.0%
CCACAGCGAAACACACTTCA+GGG - chr5_4:15809615-15809634 None:intergenic 50.0%
TCCACAGCGAAACACACTTC+AGG - chr5_4:15809616-15809635 None:intergenic 50.0%
! ACTGTGTTGCTGTGAGTGTG+AGG + chr5_4:15808255-15808274 Msa0840210:five_prime_UTR 50.0%
ATCAACAACAATGGCGGCTC+GGG + chr5_4:15808286-15808305 Msa0840210:exon 50.0%
GAGTACGCAAAGGAGATTGG+TGG - chr5_4:15808370-15808389 None:intergenic 50.0%
! GAGGAGTACGCAAAGGAGAT+TGG - chr5_4:15808373-15808392 None:intergenic 50.0%
! GAATTCGGAGGAGTACGCAA+AGG - chr5_4:15808380-15808399 None:intergenic 50.0%
CAGCGACGAAGGAAGGAATT+CGG - chr5_4:15808395-15808414 None:intergenic 50.0%
CATCAGCTCAAACGCACGTT+TGG - chr5_4:15808677-15808696 None:intergenic 50.0%
! ATAAACCCAAAAGTGCGGCG+CGG + chr5_4:15808841-15808860 Msa0840210:intron 50.0%
GTCTACACTGAGGCTCTGTT+TGG - chr5_4:15809134-15809153 None:intergenic 50.0%
GGTACACAAATGCTGGCAGA+GGG - chr5_4:15809204-15809223 None:intergenic 50.0%
AGGTTAGACTACTTCGCCTG+AGG - chr5_4:15809459-15809478 None:intergenic 50.0%
ACTTCAGGGATTTAGAGGGG+CGG - chr5_4:15809601-15809620 None:intergenic 50.0%
CCACAGCGAAACACACTTCA+GGG - chr5_4:15809615-15809634 None:intergenic 50.0%
TCCACAGCGAAACACACTTC+AGG - chr5_4:15809616-15809635 None:intergenic 50.0%
TCAACAACAATGGCGGCTCG+GGG + chr5_4:15808287-15808306 Msa0840210:exon 55.0%
CGACGAAGGAAGGAATTCGG+AGG - chr5_4:15808392-15808411 None:intergenic 55.0%
GTCGCTTCAGCGAAGAGATC+AGG + chr5_4:15808414-15808433 Msa0840210:CDS 55.0%
CGGTACACAAATGCTGGCAG+AGG - chr5_4:15809205-15809224 None:intergenic 55.0%
TTCAGGGATTTAGAGGGGCG+GGG - chr5_4:15809599-15809618 None:intergenic 55.0%
CTTCAGGGATTTAGAGGGGC+GGG - chr5_4:15809600-15809619 None:intergenic 55.0%
CCCTGAAGTGTGTTTCGCTG+TGG + chr5_4:15809612-15809631 Msa0840210:three_prime_UTR 55.0%
TGTGGATGTGCGACCTTGAG+TGG + chr5_4:15809630-15809649 Msa0840210:three_prime_UTR 55.0%
TCAACAACAATGGCGGCTCG+GGG + chr5_4:15808287-15808306 Msa0840210:exon 55.0%
CGACGAAGGAAGGAATTCGG+AGG - chr5_4:15808392-15808411 None:intergenic 55.0%
GTCGCTTCAGCGAAGAGATC+AGG + chr5_4:15808414-15808433 Msa0840210:CDS 55.0%
CGGTACACAAATGCTGGCAG+AGG - chr5_4:15809205-15809224 None:intergenic 55.0%
TTCAGGGATTTAGAGGGGCG+GGG - chr5_4:15809599-15809618 None:intergenic 55.0%
CTTCAGGGATTTAGAGGGGC+GGG - chr5_4:15809600-15809619 None:intergenic 55.0%
CCCTGAAGTGTGTTTCGCTG+TGG + chr5_4:15809612-15809631 Msa0840210:three_prime_UTR 55.0%
TGTGGATGTGCGACCTTGAG+TGG + chr5_4:15809630-15809649 Msa0840210:three_prime_UTR 55.0%
GAAGCGACAGCGACGAAGGA+AGG - chr5_4:15808402-15808421 None:intergenic 60.0%
! AGCTACCGCGCCGCACTTTT+GGG - chr5_4:15808849-15808868 None:intergenic 60.0%
! AAGCTACCGCGCCGCACTTT+TGG - chr5_4:15808850-15808869 None:intergenic 60.0%
TCAGGGATTTAGAGGGGCGG+GGG - chr5_4:15809598-15809617 None:intergenic 60.0%
GAAGCGACAGCGACGAAGGA+AGG - chr5_4:15808402-15808421 None:intergenic 60.0%
! AGCTACCGCGCCGCACTTTT+GGG - chr5_4:15808849-15808868 None:intergenic 60.0%
! AAGCTACCGCGCCGCACTTT+TGG - chr5_4:15808850-15808869 None:intergenic 60.0%
TCAGGGATTTAGAGGGGCGG+GGG - chr5_4:15809598-15809617 None:intergenic 60.0%
! ACAATGGCGGCTCGGGGAAG+CGG + chr5_4:15808293-15808312 Msa0840210:exon 65.0%
CGCTGAAGCGACAGCGACGA+AGG - chr5_4:15808406-15808425 None:intergenic 65.0%
CAGGGATTTAGAGGGGCGGG+GGG - chr5_4:15809597-15809616 None:intergenic 65.0%
! ACAATGGCGGCTCGGGGAAG+CGG + chr5_4:15808293-15808312 Msa0840210:exon 65.0%
CGCTGAAGCGACAGCGACGA+AGG - chr5_4:15808406-15808425 None:intergenic 65.0%
CAGGGATTTAGAGGGGCGGG+GGG - chr5_4:15809597-15809616 None:intergenic 65.0%
! GCGGCTCGGGGAAGCGGTTT+GGG + chr5_4:15808299-15808318 Msa0840210:CDS 70.0%
! GCGGCTCGGGGAAGCGGTTT+GGG + chr5_4:15808299-15808318 Msa0840210:CDS 70.0%
! GGCGGCTCGGGGAAGCGGTT+TGG + chr5_4:15808298-15808317 Msa0840210:CDS 75.0%
! GGCGGCTCGGGGAAGCGGTT+TGG + chr5_4:15808298-15808317 Msa0840210:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 15808229 15809864 15808229 ID=Msa0840210;Name=Msa0840210
chr5_4 mRNA 15808229 15809864 15808229 ID=Msa0840210-mRNA-1;Parent=Msa0840210;Name=Msa0840210-mRNA-1;_AED=0.01;_eAED=0.01;_QI=67|1|1|1|1|1|2|394|132
chr5_4 exon 15808229 15808517 15808229 ID=Msa0840210-mRNA-1:exon:6769;Parent=Msa0840210-mRNA-1
chr5_4 exon 15809294 15809864 15809294 ID=Msa0840210-mRNA-1:exon:6770;Parent=Msa0840210-mRNA-1
chr5_4 five_prime_UTR 15808229 15808295 15808229 ID=Msa0840210-mRNA-1:five_prime_utr;Parent=Msa0840210-mRNA-1
chr5_4 CDS 15808296 15808517 15808296 ID=Msa0840210-mRNA-1:cds;Parent=Msa0840210-mRNA-1
chr5_4 CDS 15809294 15809470 15809294 ID=Msa0840210-mRNA-1:cds;Parent=Msa0840210-mRNA-1
chr5_4 three_prime_UTR 15809471 15809864 15809471 ID=Msa0840210-mRNA-1:three_prime_utr;Parent=Msa0840210-mRNA-1
Gene Sequence

>Msa0840210

ATGGCGGCTCGGGGAAGCGGTTTGGGTCTGATGAATTACCTAAGAGTTGAGGTTCATGCTTCTGCTCTCAACCACCAATCTCCTTTGCGTACTCCTCCGAATTCCTTCCTTCGTCGCTGTCGCTTCAGCGAAGAGATCAGGAGAACTTTTCTCGACAAATCGGAGGTCACTGATCGCGTCATTTCCTGCGTCAAAAAATTTGACAATGTTGATCCTTCTAAGGTTACCCCAACTGCTCATTTCTATGATGACCTTGGATTGGATAGTTTAGACGCCATAGAGATCTGTATGGATCTTGATACAGAGTTTGGATTTGAGATTCCTGAAAATGAAGTAGACAAGATCGTCTCCGTTAATCAAGCTGTTGATTTCATTGCATCTCATCCTCAGGCGAAGTAG
Protein sequence

>Msa0840210

MAARGSGLGLMNYLRVEVHASALNHQSPLRTPPNSFLRRCRFSEEIRRTFLDKSEVTDRVISCVKKFDNVDPSKVTPTAHFYDDLGLDSLDAIEICMDLDTEFGFEIPENEVDKIVSVNQAVDFIASHPQAK*