AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0840240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840240 MtrunA17_Chr5g0409071 97.682 302 4 1 1 299 1 302 0.0 596
Msa0840240 MtrunA17_Chr4g0006261 50.000 252 110 5 44 294 40 276 1.42e-56 184
Msa0840240 MtrunA17_Chr7g0242591 49.412 255 114 5 44 297 51 291 4.79e-51 170
Msa0840240 MtrunA17_Chr3g0123021 50.820 61 30 0 46 106 77 137 5.27e-14 71.6
Msa0840240 MtrunA17_Chr2g0290511 30.508 118 71 3 30 138 241 356 7.69e-13 68.2
Msa0840240 MtrunA17_Chr1g0201911 29.752 121 75 2 22 132 342 462 1.52e-12 67.8
Msa0840240 MtrunA17_Chr1g0201941 33.696 92 56 3 46 133 455 545 3.77e-11 63.5
Msa0840240 MtrunA17_Chr6g0463941 34.066 91 55 3 46 132 465 554 7.31e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840240 AT2G38250.1 42.960 277 141 5 26 299 26 288 3.22e-49 165
Msa0840240 AT5G01380.1 39.590 293 148 6 26 299 33 315 1.23e-40 144
Msa0840240 AT1G13450.4 40.449 89 52 1 46 133 87 175 2.61e-15 73.9
Msa0840240 AT1G13450.3 40.449 89 52 1 46 133 87 175 4.16e-15 74.3
Msa0840240 AT1G13450.2 40.449 89 52 1 46 133 87 175 4.16e-15 74.3
Msa0840240 AT1G13450.1 40.449 89 52 1 46 133 87 175 1.19e-14 74.3
Msa0840240 AT3G25990.1 39.785 93 48 2 46 132 55 145 1.84e-14 73.6
Msa0840240 AT1G76890.2 34.711 121 74 3 19 135 371 490 9.20e-14 72.0
Msa0840240 AT1G76880.1 34.579 107 60 4 30 132 398 498 2.52e-11 64.7

Find 99 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAATGAGTTTGTTTAAA+AGG 0.131414 5_4:+15847938 None:intergenic
ACGTCGTGTTGATTGAAATT+AGG 0.184389 5_4:+15850136 None:intergenic
CACTTGCATTTACACTGTTC+AGG 0.240715 5_4:+15849823 None:intergenic
GTTGATTGAAATTAGGGTTT+TGG 0.241418 5_4:+15850143 None:intergenic
GGGCATTGCTTTCCATGTTC+TGG 0.245787 5_4:+15848468 None:intergenic
CGATGCTCTGGCAGCTCGCC+TGG 0.246150 5_4:-15850070 Msa0840240:CDS
CGTCGTGTTGATTGAAATTA+GGG 0.264593 5_4:+15850137 None:intergenic
CCAAAGCGTCTTGTTCCTCT+TGG 0.348352 5_4:+15849891 None:intergenic
AGATGAGGGAGAGAGGGTAT+AGG 0.349714 5_4:-15849852 Msa0840240:CDS
AGTGAAGACGGCAATATATA+AGG 0.353056 5_4:+15847576 None:intergenic
CGAGAAGCAATACACGTAAA+AGG 0.365476 5_4:-15848254 Msa0840240:CDS
GGAGAGATTAATGATTGAAC+AGG 0.371512 5_4:-15848037 Msa0840240:CDS
CAAGAGGAACAAGACGCTTT+GGG 0.378761 5_4:-15849890 Msa0840240:CDS
GAGACTGAGGGAGGAGAATA+GGG 0.379742 5_4:-15847989 Msa0840240:CDS
GGAGACTGAGGGAGGAGAAT+AGG 0.381910 5_4:-15847990 Msa0840240:CDS
GGTGAATGAAGAAAGGAGTT+AGG 0.385767 5_4:-15847765 Msa0840240:three_prime_UTR
CCAAGAGGAACAAGACGCTT+TGG 0.398905 5_4:-15849891 Msa0840240:CDS
GAATCTTGTTAATCGCTATA+AGG 0.403188 5_4:-15849797 Msa0840240:intron
TTGCATGCTATGTGCTCTTT+CGG 0.404296 5_4:+15848416 None:intergenic
GATGCTCTGGCAGCTCGCCT+GGG 0.407206 5_4:-15850069 Msa0840240:CDS
TTATTTAGGGATACATCTCT+TGG 0.410510 5_4:-15847668 Msa0840240:three_prime_UTR
ATGAATGAGTTTGTTTAAAA+GGG 0.419814 5_4:+15847939 None:intergenic
CCGAAAATGTACGGCGGCGG+TGG 0.422205 5_4:-15850099 Msa0840240:exon
ACTTGCATTTACACTGTTCA+GGG 0.425547 5_4:+15849824 None:intergenic
ATCTCTTGGGCATCAAGTCC+AGG 0.426011 5_4:-15847654 Msa0840240:three_prime_UTR
GGAAAGGATTTGAATACTAT+AGG 0.446260 5_4:-15847744 Msa0840240:three_prime_UTR
CAAGAGAGTTCATAGCGATA+AGG 0.454890 5_4:-15849942 Msa0840240:CDS
GAACAAGACGCTTTGGGAAG+TGG 0.456312 5_4:-15849884 Msa0840240:CDS
AAGAGAGTTCATAGCGATAA+GGG 0.460403 5_4:-15849941 Msa0840240:CDS
AGTTCGAAGATGAGGGAGAG+AGG 0.463651 5_4:-15849859 Msa0840240:CDS
TCAGCCGCAGTGGAGTCAAC+AGG 0.465174 5_4:-15849968 Msa0840240:CDS
CATAGCGATAAGGGCGGAGC+TGG 0.469611 5_4:-15849932 Msa0840240:CDS
ATCTAACTAAATGAGTCAAC+TGG 0.473718 5_4:-15847914 Msa0840240:exon
ACAGGAATGGCGGCAGTCAA+TGG 0.476521 5_4:-15848073 Msa0840240:CDS
AAAATGTACGGCGGCGGTGG+AGG 0.483528 5_4:-15850096 Msa0840240:CDS
AGGAGAATAGGGCAGAGAGA+AGG 0.485185 5_4:-15847978 Msa0840240:CDS
GGAGAATAGGGCAGAGAGAA+GGG 0.485668 5_4:-15847977 Msa0840240:CDS
AGTGGTGAGTTCGAAGATGA+GGG 0.492563 5_4:-15849866 Msa0840240:CDS
GATGCAGTGGAGGGAGGTGA+TGG 0.493197 5_4:-15848127 Msa0840240:CDS
ACTAAATGAGTCAACTGGCT+TGG 0.493268 5_4:-15847909 Msa0840240:exon
TGAGGGAGAGAGGGTATAGG+AGG 0.495279 5_4:-15849849 Msa0840240:CDS
AAGTGGTGAGTTCGAAGATG+AGG 0.496550 5_4:-15849867 Msa0840240:CDS
GACGGCAATATATAAGGTAT+AGG 0.502066 5_4:+15847582 None:intergenic
TTGCAACAGAGAAGTCTTCA+AGG 0.502672 5_4:-15848218 Msa0840240:CDS
TATTTAGGGATACATCTCTT+GGG 0.504408 5_4:-15847667 Msa0840240:three_prime_UTR
CGGTGGAGGAGACGATGCTC+TGG 0.506430 5_4:-15850082 Msa0840240:CDS
TGACTCCACTGCGGCTGAGC+TGG 0.508087 5_4:+15849973 None:intergenic
AACATCGTCGTTGATGTCTC+CGG 0.509011 5_4:-15850019 Msa0840240:CDS
AGTATTTGGTGAATGAAGAA+AGG 0.513284 5_4:-15847772 Msa0840240:three_prime_UTR
AACAGTTGTTTGAACAGGAA+TGG 0.513571 5_4:-15848086 Msa0840240:CDS
CTCTCCTCTCTCATCGCCGC+CGG 0.513918 5_4:+15850000 None:intergenic
GATTAATGATTGAACAGGAA+TGG 0.519107 5_4:-15848032 Msa0840240:CDS
GAGGGAGGTGATGGAAAGGC+GGG 0.529349 5_4:-15848118 Msa0840240:CDS
GGAGTGAAGAAAATAAGAAG+TGG 0.531731 5_4:-15848348 Msa0840240:CDS
GGAGGGAAAGAGAAGAGCAT+AGG 0.544277 5_4:-15848011 Msa0840240:CDS
TAATGATTGAACAGGAATGG+AGG 0.545461 5_4:-15848029 Msa0840240:CDS
CATCAAGTCCAGGGATATGT+TGG 0.547748 5_4:-15847644 Msa0840240:three_prime_UTR
CGATAAGGGCGGAGCTGGAG+AGG 0.548497 5_4:-15849927 Msa0840240:CDS
TTACGTGTATTGCTTCTCGC+AGG 0.548915 5_4:+15848258 None:intergenic
AATACACGTAAAAGGAAAGC+TGG 0.551659 5_4:-15848246 Msa0840240:CDS
GGGTACCAGCTCAGCCGCAG+TGG 0.554507 5_4:-15849978 Msa0840240:CDS
GATAAGGGCGGAGCTGGAGA+GGG 0.555293 5_4:-15849926 Msa0840240:CDS
GTTCGAAGATGAGGGAGAGA+GGG 0.555989 5_4:-15849858 Msa0840240:CDS
AATGGAGATGCAGTGGAGGG+AGG 0.556920 5_4:-15848133 Msa0840240:CDS
CTTTACAACCAACATATCCC+TGG 0.557408 5_4:+15847636 None:intergenic
GGAGGGAGGTGATGGAAAGG+CGG 0.558316 5_4:-15848119 Msa0840240:CDS
ATGCTCTGGCAGCTCGCCTG+GGG 0.558467 5_4:-15850068 Msa0840240:CDS
GGAGACATCAACGACGATGT+TGG 0.560523 5_4:+15850021 None:intergenic
GTGACGGTGGTACTATCCCC+AGG 0.560653 5_4:+15850052 None:intergenic
AATGATTGAACAGGAATGGA+GGG 0.570661 5_4:-15848028 Msa0840240:CDS
CTGAACAGTGTAAATGCAAG+TGG 0.574316 5_4:-15849822 Msa0840240:CDS
AGTTGAAGATGACAGTGAAG+AGG 0.575058 5_4:-15848286 Msa0840240:CDS
TCTCTTGGGCATCAAGTCCA+GGG 0.577482 5_4:-15847653 Msa0840240:three_prime_UTR
CTGAGAATTCGTCAGAGGAT+CGG 0.577596 5_4:+15848322 None:intergenic
CGAATTCTCAGAAGATGATG+AGG 0.577887 5_4:-15848310 Msa0840240:CDS
ATGAAGAAAGGAGTTAGGAA+AGG 0.595085 5_4:-15847760 Msa0840240:three_prime_UTR
GAGACATCTGATCCAGAACA+TGG 0.596082 5_4:-15848480 Msa0840240:CDS
GAGAAGAGCATAGGAGACTG+AGG 0.597643 5_4:-15848002 Msa0840240:CDS
TGACAGAAACCGAAAATGTA+CGG 0.599687 5_4:-15850108 Msa0840240:exon
AGTCAATGGAAAAGCTTGAG+AGG 0.603425 5_4:-15848059 Msa0840240:CDS
GGCGGCGATGAGAGAGGAGA+GGG 0.609377 5_4:-15849998 Msa0840240:CDS
AGTGGAGGGAGGTGATGGAA+AGG 0.613399 5_4:-15848122 Msa0840240:CDS
GTCAATGGAAAAGCTTGAGA+GGG 0.615909 5_4:-15848058 Msa0840240:CDS
GTCTCCGGCGGCGATGAGAG+AGG 0.619988 5_4:-15850004 Msa0840240:CDS
TGCAACAGAGAAGTCTTCAA+GGG 0.632169 5_4:-15848217 Msa0840240:CDS
TAATATTCAAGAAATGCTCA+AGG 0.634081 5_4:-15848178 Msa0840240:CDS
AGAAGAGCATAGGAGACTGA+GGG 0.643488 5_4:-15848001 Msa0840240:CDS
ATCTTCTGAGAATTCGTCAG+AGG 0.649927 5_4:+15848317 None:intergenic
CGGCGGCGATGAGAGAGGAG+AGG 0.655250 5_4:-15849999 Msa0840240:CDS
AGAGCATAGGAGACTGAGGG+AGG 0.658874 5_4:-15847998 Msa0840240:CDS
GGGATTTCACTGCTTCCAAG+AGG 0.659081 5_4:-15849906 Msa0840240:CDS
ATCGTCGTTGATGTCTCCGG+CGG 0.659235 5_4:-15850016 Msa0840240:CDS
GATGTTGGAATCAGAAGTGA+CGG 0.659264 5_4:+15850036 None:intergenic
AGTTGTTTGAACAGGAATGG+CGG 0.675425 5_4:-15848083 Msa0840240:CDS
AAACCGAAAATGTACGGCGG+CGG 0.675954 5_4:-15850102 Msa0840240:exon
AGAGTTCATAGCGATAAGGG+CGG 0.691633 5_4:-15849938 Msa0840240:CDS
GTTGGAATCAGAAGTGACGG+TGG 0.741050 5_4:+15850039 None:intergenic
CAGAAACCGAAAATGTACGG+CGG 0.798682 5_4:-15850105 Msa0840240:exon
GTCTCCTGTTGACTCCACTG+CGG 0.804513 5_4:+15849964 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATATATGATTTTAAGTT+TGG - chr5_4:15849959-15849978 Msa0840240:CDS 10.0%
!! CAAAAAATGATATAATTGTA+AGG + chr5_4:15848368-15848387 None:intergenic 15.0%
!!! TTTGTAAGTTTTTTTTTGTT+TGG + chr5_4:15848528-15848547 None:intergenic 15.0%
!! ACTGAAAAATGAAAATGAAA+AGG + chr5_4:15847630-15847649 None:intergenic 20.0%
!!! TATCTTTCTCTTTATGTTTT+GGG - chr5_4:15848037-15848056 Msa0840240:CDS 20.0%
!! TGCATTGTTATAATTTCATT+TGG - chr5_4:15848270-15848289 Msa0840240:CDS 20.0%
!!! AAACAATTTTTGAATTTGCA+GGG - chr5_4:15848495-15848514 Msa0840240:intron 20.0%
!! AATTCAAAAACTAATGCTTT+CGG + chr5_4:15848553-15848572 None:intergenic 20.0%
!! ATGGTAATAAAAACAAATGT+TGG + chr5_4:15848581-15848600 None:intergenic 20.0%
!!! CATTTGTTTTTATTACCATT+TGG - chr5_4:15848582-15848601 Msa0840240:intron 20.0%
!! AAAAGCTATATCTTGAATAT+TGG - chr5_4:15849254-15849273 Msa0840240:intron 20.0%
!! AAAGCTATATCTTGAATATT+GGG - chr5_4:15849255-15849274 Msa0840240:intron 20.0%
!!! ATGAATGAGTTTGTTTAAAA+GGG + chr5_4:15849875-15849894 None:intergenic 20.0%
!!! AGCTTTTGTATTGATTATTT+AGG - chr5_4:15850129-15850148 Msa0840240:five_prime_UTR 20.0%
!!! GCTTTTGTATTGATTATTTA+GGG - chr5_4:15850130-15850149 Msa0840240:five_prime_UTR 20.0%
!! TTGATTGAAATTAGGGTTTT+GGG + chr5_4:15847670-15847689 None:intergenic 25.0%
!! GTATCTTTCTCTTTATGTTT+TGG - chr5_4:15848036-15848055 Msa0840240:CDS 25.0%
!!! TCTTTATGTTTTGGGTTTTT+AGG - chr5_4:15848045-15848064 Msa0840240:CDS 25.0%
!!! CTTTATGTTTTGGGTTTTTA+GGG - chr5_4:15848046-15848065 Msa0840240:CDS 25.0%
!! ACTAAGTTTTTGAATTTGCA+GGG + chr5_4:15848427-15848446 None:intergenic 25.0%
! AATTCAAAAACTTAGTCTCT+TGG - chr5_4:15848431-15848450 Msa0840240:CDS 25.0%
!! GAAACAATTTTTGAATTTGC+AGG - chr5_4:15848494-15848513 Msa0840240:intron 25.0%
! ATATATGAAATGCTGTGATT+TGG - chr5_4:15848650-15848669 Msa0840240:intron 25.0%
! TGAGGACTAAAAAGTTATAT+TGG + chr5_4:15848941-15848960 None:intergenic 25.0%
!!! ATATAACTTTTTAGTCCTCA+AGG - chr5_4:15848941-15848960 Msa0840240:intron 25.0%
!!! AACATGTATGATTTGGTATT+TGG - chr5_4:15849142-15849161 Msa0840240:intron 25.0%
! AGTAAATTTAATCATGTCGT+TGG - chr5_4:15849173-15849192 Msa0840240:intron 25.0%
! GTAAATTTAATCATGTCGTT+GGG - chr5_4:15849174-15849193 Msa0840240:intron 25.0%
! AAGCTATATCTTGAATATTG+GGG - chr5_4:15849256-15849275 Msa0840240:intron 25.0%
! CATGCAATTCTTCAAAAAAT+GGG + chr5_4:15849366-15849385 None:intergenic 25.0%
! TAATATTCAAGAAATGCTCA+AGG - chr5_4:15849633-15849652 Msa0840240:intron 25.0%
! TTTCCAACACCAATTAAAAA+TGG - chr5_4:15849660-15849679 Msa0840240:intron 25.0%
!! CATGAATGAGTTTGTTTAAA+AGG + chr5_4:15849876-15849895 None:intergenic 25.0%
!!! CTTTTGATTACAGAGAATTT+TGG - chr5_4:15850011-15850030 Msa0840240:CDS 25.0%
!!! TTTCTTTTGAAAGTTGATAC+AGG - chr5_4:15850103-15850122 Msa0840240:exon 25.0%
!!! AAATTAGGGTTTTGGGAAAT+TGG + chr5_4:15847663-15847682 None:intergenic 30.0%
! GTTGATTGAAATTAGGGTTT+TGG + chr5_4:15847671-15847690 None:intergenic 30.0%
GAATCTTGTTAATCGCTATA+AGG - chr5_4:15848014-15848033 Msa0840240:CDS 30.0%
GAAATATGCATTTGTAGTGT+TGG - chr5_4:15848104-15848123 Msa0840240:CDS 30.0%
! GACTAAGTTTTTGAATTTGC+AGG + chr5_4:15848428-15848447 None:intergenic 30.0%
! TTTTATTACCATTTGGACTG+TGG - chr5_4:15848589-15848608 Msa0840240:intron 30.0%
TTATTTATCCACAGTCCAAA+TGG + chr5_4:15848600-15848619 None:intergenic 30.0%
ATAAGTATGGACCATAATGT+TGG - chr5_4:15848616-15848635 Msa0840240:intron 30.0%
TAAGTATGGACCATAATGTT+GGG - chr5_4:15848617-15848636 Msa0840240:intron 30.0%
AACATTGAAGGAGTTTGAAA+AGG - chr5_4:15848987-15849006 Msa0840240:intron 30.0%
AACCTCTAACATGTATGATT+TGG - chr5_4:15849135-15849154 Msa0840240:intron 30.0%
TACCAAATCATACATGTTAG+AGG + chr5_4:15849140-15849159 None:intergenic 30.0%
GCATGCAATTCTTCAAAAAA+TGG + chr5_4:15849367-15849386 None:intergenic 30.0%
!! TCTCCATTTTTAATTGGTGT+TGG + chr5_4:15849666-15849685 None:intergenic 30.0%
!! ACTGCATCTCCATTTTTAAT+TGG + chr5_4:15849672-15849691 None:intergenic 30.0%
AATTAAAAATGGAGATGCAG+TGG - chr5_4:15849671-15849690 Msa0840240:intron 30.0%
TGAAAAACAGTTGTTTGAAC+AGG - chr5_4:15849720-15849739 Msa0840240:intron 30.0%
GATTAATGATTGAACAGGAA+TGG - chr5_4:15849779-15849798 Msa0840240:intron 30.0%
ATCTAACTAAATGAGTCAAC+TGG - chr5_4:15849897-15849916 Msa0840240:CDS 30.0%
!! GATTACAGAGAATTTTGGAA+AGG - chr5_4:15850016-15850035 Msa0840240:CDS 30.0%
!!! GAATTTTGGAAAGGAGTATT+TGG - chr5_4:15850025-15850044 Msa0840240:CDS 30.0%
! AGTATTTGGTGAATGAAGAA+AGG - chr5_4:15850039-15850058 Msa0840240:CDS 30.0%
GGAAAGGATTTGAATACTAT+AGG - chr5_4:15850067-15850086 Msa0840240:CDS 30.0%
TTATTTAGGGATACATCTCT+TGG - chr5_4:15850143-15850162 Msa0840240:five_prime_UTR 30.0%
TATTTAGGGATACATCTCTT+GGG - chr5_4:15850144-15850163 Msa0840240:five_prime_UTR 30.0%
!! CGTCGTGTTGATTGAAATTA+GGG + chr5_4:15847677-15847696 None:intergenic 35.0%
!! ACGTCGTGTTGATTGAAATT+AGG + chr5_4:15847678-15847697 None:intergenic 35.0%
TGACAGAAACCGAAAATGTA+CGG - chr5_4:15847703-15847722 Msa0840240:three_prime_UTR 35.0%
AAGAGAGTTCATAGCGATAA+GGG - chr5_4:15847870-15847889 Msa0840240:three_prime_UTR 35.0%
ACTTGCATTTACACTGTTCA+GGG + chr5_4:15847990-15848009 None:intergenic 35.0%
GGACTGTGGATAAATAAGTA+TGG - chr5_4:15848603-15848622 Msa0840240:intron 35.0%
ATGAACTCAACCCAACATTA+TGG + chr5_4:15848630-15848649 None:intergenic 35.0%
!! ATGAAATGCTGTGATTTGGT+TGG - chr5_4:15848654-15848673 Msa0840240:intron 35.0%
ATAATGAGGTCCAGTTCAAA+TGG + chr5_4:15848707-15848726 None:intergenic 35.0%
TCAAATGCTAACTTGAGCTT+CGG - chr5_4:15848882-15848901 Msa0840240:intron 35.0%
ATTGAAGGAGTTTGAAAAGG+AGG - chr5_4:15848990-15849009 Msa0840240:intron 35.0%
! AGGTTTTCTGCATATGCAAA+GGG + chr5_4:15849120-15849139 None:intergenic 35.0%
AAAGAAAACTGATGCACAGT+TGG + chr5_4:15849217-15849236 None:intergenic 35.0%
! TCACATGATGATGTTTATGC+AGG - chr5_4:15849303-15849322 Msa0840240:intron 35.0%
! CACATGATGATGTTTATGCA+GGG - chr5_4:15849304-15849323 Msa0840240:intron 35.0%
CGTTCAGCTCAAACAAAAAA+AGG - chr5_4:15849442-15849461 Msa0840240:intron 35.0%
GGAGTGAAGAAAATAAGAAG+TGG - chr5_4:15849463-15849482 Msa0840240:intron 35.0%
AATACACGTAAAAGGAAAGC+TGG - chr5_4:15849565-15849584 Msa0840240:intron 35.0%
AACAGTTGTTTGAACAGGAA+TGG - chr5_4:15849725-15849744 Msa0840240:intron 35.0%
GGAGAGATTAATGATTGAAC+AGG - chr5_4:15849774-15849793 Msa0840240:intron 35.0%
TAATGATTGAACAGGAATGG+AGG - chr5_4:15849782-15849801 Msa0840240:intron 35.0%
AATGATTGAACAGGAATGGA+GGG - chr5_4:15849783-15849802 Msa0840240:intron 35.0%
ATGAAGAAAGGAGTTAGGAA+AGG - chr5_4:15850051-15850070 Msa0840240:CDS 35.0%
GATGTTGGAATCAGAAGTGA+CGG + chr5_4:15847778-15847797 None:intergenic 40.0%
CAAGAGAGTTCATAGCGATA+AGG - chr5_4:15847869-15847888 Msa0840240:three_prime_UTR 40.0%
CACTTGCATTTACACTGTTC+AGG + chr5_4:15847991-15848010 None:intergenic 40.0%
CTGAACAGTGTAAATGCAAG+TGG - chr5_4:15847989-15848008 Msa0840240:CDS 40.0%
!! TAGTCTCTTGGTGTCATACA+TGG - chr5_4:15848443-15848462 Msa0840240:CDS 40.0%
GTCCTTAACGTCAGCTTATT+GGG + chr5_4:15848469-15848488 None:intergenic 40.0%
AGTCCTTAACGTCAGCTTAT+TGG + chr5_4:15848470-15848489 None:intergenic 40.0%
!! AAATGCTGTGATTTGGTTGG+AGG - chr5_4:15848657-15848676 Msa0840240:intron 40.0%
!! AATGCTGTGATTTGGTTGGA+GGG - chr5_4:15848658-15848677 Msa0840240:intron 40.0%
TTCTGAATCGCTCTAGTTAG+GGG + chr5_4:15848740-15848759 None:intergenic 40.0%
CTTCTGAATCGCTCTAGTTA+GGG + chr5_4:15848741-15848760 None:intergenic 40.0%
! TCTTGATGGGAAAGTTGGTT+GGG + chr5_4:15848792-15848811 None:intergenic 40.0%
! TTCTTGATGGGAAAGTTGGT+TGG + chr5_4:15848793-15848812 None:intergenic 40.0%
AGGTTTCTTGATGGGAAAGT+TGG + chr5_4:15848797-15848816 None:intergenic 40.0%
GCTTGACAAGGTTTCTTGAT+GGG + chr5_4:15848805-15848824 None:intergenic 40.0%
TCCTCAAGGTGTAGTTCAAT+TGG - chr5_4:15848955-15848974 Msa0840240:intron 40.0%
ACCAATTGAACTACACCTTG+AGG + chr5_4:15848959-15848978 None:intergenic 40.0%
TGGTTGAGCTGAAACATTGA+AGG - chr5_4:15848975-15848994 Msa0840240:intron 40.0%
! GGTTTTCTGCATATGCAAAG+GGG + chr5_4:15849119-15849138 None:intergenic 40.0%
! GAGGTTTTCTGCATATGCAA+AGG + chr5_4:15849121-15849140 None:intergenic 40.0%
TTGCATGCTATGTGCTCTTT+CGG + chr5_4:15849398-15849417 None:intergenic 40.0%
! ATCTTCTGAGAATTCGTCAG+AGG + chr5_4:15849497-15849516 None:intergenic 40.0%
CGAATTCTCAGAAGATGATG+AGG - chr5_4:15849501-15849520 Msa0840240:intron 40.0%
AGTTGAAGATGACAGTGAAG+AGG - chr5_4:15849525-15849544 Msa0840240:intron 40.0%
CGAGAAGCAATACACGTAAA+AGG - chr5_4:15849557-15849576 Msa0840240:intron 40.0%
TTGCAACAGAGAAGTCTTCA+AGG - chr5_4:15849593-15849612 Msa0840240:intron 40.0%
TGCAACAGAGAAGTCTTCAA+GGG - chr5_4:15849594-15849613 Msa0840240:intron 40.0%
TAAAAATGGAGATGCAGTGG+AGG - chr5_4:15849674-15849693 Msa0840240:intron 40.0%
AAAAATGGAGATGCAGTGGA+GGG - chr5_4:15849675-15849694 Msa0840240:intron 40.0%
AGTTGTTTGAACAGGAATGG+CGG - chr5_4:15849728-15849747 Msa0840240:intron 40.0%
AGTCAATGGAAAAGCTTGAG+AGG - chr5_4:15849752-15849771 Msa0840240:intron 40.0%
GTCAATGGAAAAGCTTGAGA+GGG - chr5_4:15849753-15849772 Msa0840240:intron 40.0%
ACTAAATGAGTCAACTGGCT+TGG - chr5_4:15849902-15849921 Msa0840240:CDS 40.0%
GGTGAATGAAGAAAGGAGTT+AGG - chr5_4:15850046-15850065 Msa0840240:CDS 40.0%
CTTTACAACCAACATATCCC+TGG + chr5_4:15850178-15850197 None:intergenic 40.0%
CAGAAACCGAAAATGTACGG+CGG - chr5_4:15847706-15847725 Msa0840240:three_prime_UTR 45.0%
AACATCGTCGTTGATGTCTC+CGG - chr5_4:15847792-15847811 Msa0840240:three_prime_UTR 45.0%
AGAGTTCATAGCGATAAGGG+CGG - chr5_4:15847873-15847892 Msa0840240:three_prime_UTR 45.0%
CAAGAGGAACAAGACGCTTT+GGG - chr5_4:15847921-15847940 Msa0840240:exon 45.0%
AAGTGGTGAGTTCGAAGATG+AGG - chr5_4:15847944-15847963 Msa0840240:CDS 45.0%
AGTGGTGAGTTCGAAGATGA+GGG - chr5_4:15847945-15847964 Msa0840240:CDS 45.0%
! TTTGGTTGGAGGGTGTGTTA+TGG - chr5_4:15848668-15848687 Msa0840240:intron 45.0%
! TTGGAGGGTGTGTTATGGAT+TGG - chr5_4:15848673-15848692 Msa0840240:intron 45.0%
! TGGAGGGTGTGTTATGGATT+GGG - chr5_4:15848674-15848693 Msa0840240:intron 45.0%
GCTTCTGAATCGCTCTAGTT+AGG + chr5_4:15848742-15848761 None:intergenic 45.0%
CACATAATGGTGCAGTGATG+TGG + chr5_4:15848768-15848787 None:intergenic 45.0%
! AAGTTGGTTGGGGCACATAA+TGG + chr5_4:15848781-15848800 None:intergenic 45.0%
! CTTGATGGGAAAGTTGGTTG+GGG + chr5_4:15848791-15848810 None:intergenic 45.0%
CGCTTGACAAGGTTTCTTGA+TGG + chr5_4:15848806-15848825 None:intergenic 45.0%
AAGAGTGATCTCGCTTGACA+AGG + chr5_4:15848817-15848836 None:intergenic 45.0%
GGAGTTTGAAAAGGAGGTCT+AGG - chr5_4:15848996-15849015 Msa0840240:intron 45.0%
GAGGTCAATATGCACTAGTG+AGG + chr5_4:15849097-15849116 None:intergenic 45.0%
TTTCTGCATATGCAAAGGGG+AGG + chr5_4:15849116-15849135 None:intergenic 45.0%
GAGACATCTGATCCAGAACA+TGG - chr5_4:15849331-15849350 Msa0840240:intron 45.0%
CTGAGAATTCGTCAGAGGAT+CGG + chr5_4:15849492-15849511 None:intergenic 45.0%
TTACGTGTATTGCTTCTCGC+AGG + chr5_4:15849556-15849575 None:intergenic 45.0%
! AGAAGAGCATAGGAGACTGA+GGG - chr5_4:15849810-15849829 Msa0840240:CDS 45.0%
CATCAAGTCCAGGGATATGT+TGG - chr5_4:15850167-15850186 Msa0840240:five_prime_UTR 45.0%
AAACCGAAAATGTACGGCGG+CGG - chr5_4:15847709-15847728 Msa0840240:three_prime_UTR 50.0%
GTTGGAATCAGAAGTGACGG+TGG + chr5_4:15847775-15847794 None:intergenic 50.0%
GGAGACATCAACGACGATGT+TGG + chr5_4:15847793-15847812 None:intergenic 50.0%
GGGATTTCACTGCTTCCAAG+AGG - chr5_4:15847905-15847924 Msa0840240:three_prime_UTR 50.0%
! CCAAAGCGTCTTGTTCCTCT+TGG + chr5_4:15847923-15847942 None:intergenic 50.0%
CCAAGAGGAACAAGACGCTT+TGG - chr5_4:15847920-15847939 Msa0840240:exon 50.0%
!! GAACAAGACGCTTTGGGAAG+TGG - chr5_4:15847927-15847946 Msa0840240:CDS 50.0%
AGTTCGAAGATGAGGGAGAG+AGG - chr5_4:15847952-15847971 Msa0840240:CDS 50.0%
GTTCGAAGATGAGGGAGAGA+GGG - chr5_4:15847953-15847972 Msa0840240:CDS 50.0%
! AGATGAGGGAGAGAGGGTAT+AGG - chr5_4:15847959-15847978 Msa0840240:CDS 50.0%
GGCCCAATAAGCTGACGTTA+AGG - chr5_4:15848464-15848483 Msa0840240:CDS 50.0%
!! GGTGTGTTATGGATTGGGTC+AGG - chr5_4:15848679-15848698 Msa0840240:intron 50.0%
GGGTCAGGATCCATTTGAAC+TGG - chr5_4:15848694-15848713 Msa0840240:intron 50.0%
GAATCGCTCTAGTTAGGGGT+GGG + chr5_4:15848736-15848755 None:intergenic 50.0%
TGAATCGCTCTAGTTAGGGG+TGG + chr5_4:15848737-15848756 None:intergenic 50.0%
AGCTCCTTCAACCTGCCAAA+CGG - chr5_4:15849071-15849090 Msa0840240:intron 50.0%
TATGCACTAGTGAGGCCGTT+TGG + chr5_4:15849089-15849108 None:intergenic 50.0%
! GGGCATTGCTTTCCATGTTC+TGG + chr5_4:15849346-15849365 None:intergenic 50.0%
! GGAGGGAAAGAGAAGAGCAT+AGG - chr5_4:15849800-15849819 Msa0840240:CDS 50.0%
! GAGAAGAGCATAGGAGACTG+AGG - chr5_4:15849809-15849828 Msa0840240:CDS 50.0%
GAGACTGAGGGAGGAGAATA+GGG - chr5_4:15849822-15849841 Msa0840240:CDS 50.0%
AGGAGAATAGGGCAGAGAGA+AGG - chr5_4:15849833-15849852 Msa0840240:CDS 50.0%
GGAGAATAGGGCAGAGAGAA+GGG - chr5_4:15849834-15849853 Msa0840240:CDS 50.0%
ATCTCTTGGGCATCAAGTCC+AGG - chr5_4:15850157-15850176 Msa0840240:five_prime_UTR 50.0%
TCTCTTGGGCATCAAGTCCA+GGG - chr5_4:15850158-15850177 Msa0840240:five_prime_UTR 50.0%
ATCGTCGTTGATGTCTCCGG+CGG - chr5_4:15847795-15847814 Msa0840240:three_prime_UTR 55.0%
!! GTCTCCTGTTGACTCCACTG+CGG + chr5_4:15847850-15847869 None:intergenic 55.0%
! TGAGGGAGAGAGGGTATAGG+AGG - chr5_4:15847962-15847981 Msa0840240:CDS 55.0%
ATGCAACCACCCCACTTCCT+TGG - chr5_4:15848183-15848202 Msa0840240:CDS 55.0%
AACCACCCCACTTCCTTGGT+GGG - chr5_4:15848187-15848206 Msa0840240:CDS 55.0%
CTTGTCCCACCAAGGAAGTG+GGG + chr5_4:15848195-15848214 None:intergenic 55.0%
CCTTGTCCCACCAAGGAAGT+GGG + chr5_4:15848196-15848215 None:intergenic 55.0%
! AACGAAGCCTTGTCCCACCA+AGG + chr5_4:15848203-15848222 None:intergenic 55.0%
! GGGGTGGGCTTCACATAATG+AGG + chr5_4:15848721-15848740 None:intergenic 55.0%
AATGGAGATGCAGTGGAGGG+AGG - chr5_4:15849678-15849697 Msa0840240:intron 55.0%
!! AGTGGAGGGAGGTGATGGAA+AGG - chr5_4:15849689-15849708 Msa0840240:intron 55.0%
ACAGGAATGGCGGCAGTCAA+TGG - chr5_4:15849738-15849757 Msa0840240:intron 55.0%
AGAGCATAGGAGACTGAGGG+AGG - chr5_4:15849813-15849832 Msa0840240:CDS 55.0%
GGAGACTGAGGGAGGAGAAT+AGG - chr5_4:15849821-15849840 Msa0840240:CDS 55.0%
! CCACCGCCGCCGTACATTTT+CGG + chr5_4:15847715-15847734 None:intergenic 60.0%
AAAATGTACGGCGGCGGTGG+AGG - chr5_4:15847715-15847734 Msa0840240:three_prime_UTR 60.0%
GTGACGGTGGTACTATCCCC+AGG + chr5_4:15847762-15847781 None:intergenic 60.0%
TCAGCCGCAGTGGAGTCAAC+AGG - chr5_4:15847843-15847862 Msa0840240:three_prime_UTR 60.0%
CATAGCGATAAGGGCGGAGC+TGG - chr5_4:15847879-15847898 Msa0840240:three_prime_UTR 60.0%
GATAAGGGCGGAGCTGGAGA+GGG - chr5_4:15847885-15847904 Msa0840240:three_prime_UTR 60.0%
CAACCACCCCACTTCCTTGG+TGG - chr5_4:15848186-15848205 Msa0840240:CDS 60.0%
CCCACTTCCTTGGTGGGACA+AGG - chr5_4:15848193-15848212 Msa0840240:CDS 60.0%
GCCTTGTCCCACCAAGGAAG+TGG + chr5_4:15848197-15848216 None:intergenic 60.0%
! GAGGCCGTTTGGCAGGTTGA+AGG + chr5_4:15849078-15849097 None:intergenic 60.0%
CACTAGTGAGGCCGTTTGGC+AGG + chr5_4:15849085-15849104 None:intergenic 60.0%
GATGCAGTGGAGGGAGGTGA+TGG - chr5_4:15849684-15849703 Msa0840240:intron 60.0%
!! GGAGGGAGGTGATGGAAAGG+CGG - chr5_4:15849692-15849711 Msa0840240:intron 60.0%
!! GAGGGAGGTGATGGAAAGGC+GGG - chr5_4:15849693-15849712 Msa0840240:intron 60.0%
CCGAAAATGTACGGCGGCGG+TGG - chr5_4:15847712-15847731 Msa0840240:three_prime_UTR 65.0%
CGGTGGAGGAGACGATGCTC+TGG - chr5_4:15847729-15847748 Msa0840240:three_prime_UTR 65.0%
GATGCTCTGGCAGCTCGCCT+GGG - chr5_4:15847742-15847761 Msa0840240:three_prime_UTR 65.0%
ATGCTCTGGCAGCTCGCCTG+GGG - chr5_4:15847743-15847762 Msa0840240:three_prime_UTR 65.0%
CTCTCCTCTCTCATCGCCGC+CGG + chr5_4:15847814-15847833 None:intergenic 65.0%
GGCGGCGATGAGAGAGGAGA+GGG - chr5_4:15847813-15847832 Msa0840240:three_prime_UTR 65.0%
TGACTCCACTGCGGCTGAGC+TGG + chr5_4:15847841-15847860 None:intergenic 65.0%
CGATAAGGGCGGAGCTGGAG+AGG - chr5_4:15847884-15847903 Msa0840240:three_prime_UTR 65.0%
GTCCCACCAAGGAAGTGGGG+TGG + chr5_4:15848192-15848211 None:intergenic 65.0%
CGATGCTCTGGCAGCTCGCC+TGG - chr5_4:15847741-15847760 Msa0840240:three_prime_UTR 70.0%
GTCTCCGGCGGCGATGAGAG+AGG - chr5_4:15847807-15847826 Msa0840240:three_prime_UTR 70.0%
CGGCGGCGATGAGAGAGGAG+AGG - chr5_4:15847812-15847831 Msa0840240:three_prime_UTR 70.0%
GGGTACCAGCTCAGCCGCAG+TGG - chr5_4:15847833-15847852 Msa0840240:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 15847584 15850249 15847584 ID=Msa0840240;Name=Msa0840240
chr5_4 mRNA 15847584 15850249 15847584 ID=Msa0840240-mRNA-1;Parent=Msa0840240;Name=Msa0840240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=134|1|1|1|1|1|2|343|299
chr5_4 exon 15847584 15848508 15847584 ID=Msa0840240-mRNA-1:exon:6775;Parent=Msa0840240-mRNA-1
chr5_4 exon 15849798 15850249 15849798 ID=Msa0840240-mRNA-1:exon:6774;Parent=Msa0840240-mRNA-1
chr5_4 five_prime_UTR 15850116 15850249 15850116 ID=Msa0840240-mRNA-1:five_prime_utr;Parent=Msa0840240-mRNA-1
chr5_4 CDS 15849798 15850115 15849798 ID=Msa0840240-mRNA-1:cds;Parent=Msa0840240-mRNA-1
chr5_4 CDS 15847927 15848508 15847927 ID=Msa0840240-mRNA-1:cds;Parent=Msa0840240-mRNA-1
chr5_4 three_prime_UTR 15847584 15847926 15847584 ID=Msa0840240-mRNA-1:three_prime_utr;Parent=Msa0840240-mRNA-1
Gene Sequence

>Msa0840240

ATGTACGGCGGCGGTGGAGGAGACGATGCTCTGGCAGCTCGCCTGGGGATAGTACCACCGTCACTTCTGATTCCAACATCGTCGTTGATGTCTCCGGCGGCGATGAGAGAGGAGAGGGTACCAGCTCAGCCGCAGTGGAGTCAACAGGAGACAAGAGAGTTCATAGCGATAAGGGCGGAGCTGGAGAGGGATTTCACTGCTTCCAAGAGGAACAAGACGCTTTGGGAAGTGGTGAGTTCGAAGATGAGGGAGAGAGGGTATAGGAGGAGCCCTGAACAGTGTAAATGCAAGTGGAAGAATCTTGTTAATCGCTATAAGGGAAAAGAGACATCTGATCCAGAACATGGAAAGCAATGCCCATTTTTTGAAGAATTGCATGCAGTTTTTACCGAAAGAGCACATAGCATGCAACGCTTACTTCTTGAATCTGAAACTCGTTCAGCTCAAACAAAAAAAGGAGTGAAGAAAATAAGAAGTGGAGACCGATCCTCTGACGAATTCTCAGAAGATGATGAGGAAGTTGAAGATGACAGTGAAGAGGAGAGACCTGCGAGAAGCAATACACGTAAAAGGAAAGCTGGCAAAATTGCAACAGAGAAGTCTTCAAGGGCAAACAATAGTACTAGTAATATTCAAGAAATGCTCAAGGAATTTTTCCAACACCAATTAAAAATGGAGATGCAGTGGAGGGAGGTGATGGAAAGGCGGGCACATGAAAAACAGTTGTTTGAACAGGAATGGCGGCAGTCAATGGAAAAGCTTGAGAGGGAGAGATTAATGATTGAACAGGAATGGAGGGAAAGAGAAGAGCATAGGAGACTGAGGGAGGAGAATAGGGCAGAGAGAAGGGACGCTTTGTTGACGACCCTTTTAAACAAACTCATTCATGAATCTAACTAA
Protein sequence

>Msa0840240

MYGGGGGDDALAARLGIVPPSLLIPTSSLMSPAAMREERVPAQPQWSQQETREFIAIRAELERDFTASKRNKTLWEVVSSKMRERGYRRSPEQCKCKWKNLVNRYKGKETSDPEHGKQCPFFEELHAVFTERAHSMQRLLLESETRSAQTKKGVKKIRSGDRSSDEFSEDDEEVEDDSEEERPARSNTRKRKAGKIATEKSSRANNSTSNIQEMLKEFFQHQLKMEMQWREVMERRAHEKQLFEQEWRQSMEKLERERLMIEQEWREREEHRRLREENRAERRDALLTTLLNKLIHESN*